BLASTP 2.2.16 [Mar-25-2007]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BAB81731.1 cper0 CPE2025 GIB00075CH01 "conserved hypothetical
protein"
(434 letters)
Database: sws57.9x
510,076 sequences; 179,409,320 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
YQEV_BACSU RecName: Full=Putative methylthiotransferase yqeV; ... 363 e-102
Y474_AQUAE RecName: Full=Putative methylthiotransferase aq_474; ... 318 4e-89
Y16R_MYCIO RecName: Full=Putative methylthiotransferase in 16S R... 273 2e-75
MIAB_CLOTE RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 261 1e-71
MIAB_CLOBM RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 259 2e-71
MIAB_CLOBK RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 258 5e-71
Y830_THEMA RecName: Full=Putative methylthiotransferase TM_0830;... 258 9e-71
MIAB_THEP3 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 256 3e-70
MIAB_THEPX RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 255 6e-70
MIAB_PELTS RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 253 2e-69
MIAB_CLOBH RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 252 4e-69
MIAB_CLOB1 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 252 4e-69
MIAB_CLOBL RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 248 5e-68
MIAB_CLOB8 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 246 2e-67
MIAB_THETN RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 244 1e-66
MIAB_CLOTH RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 242 4e-66
MIAB_CLOBB RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 242 4e-66
MIAB_CLOBA RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 242 5e-66
MIAB_CLONN RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 241 1e-65
MIAB_ALKOO RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 239 3e-65
MIAB_CLOAB RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 238 6e-65
MIAB_NATTJ RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 237 1e-64
MIAB_CLOPH RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 233 2e-63
MIAB_CLOPE RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 231 7e-63
MIAB_CLOP1 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 231 7e-63
MIAB_CLOPS RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 231 9e-63
Y477_CHLPN RecName: Full=Putative methylthiotransferase CPn_0477... 229 4e-62
MIAB_AKKM8 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 228 1e-61
MIAB_FINM2 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 227 1e-61
MIAB_BACCN RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 226 3e-61
MIAB_CARHZ RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 225 5e-61
MIAB_BACWK RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 224 8e-61
MIAB_DESAP RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 224 1e-60
MIAB_STAAW RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 223 2e-60
MIAB_STAAS RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 223 2e-60
MIAB_STAAN RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 223 2e-60
MIAB_STAAM RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 223 2e-60
MIAB_STAA9 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 223 2e-60
MIAB_STAA2 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 223 2e-60
MIAB_STAA1 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 223 2e-60
MIAB_BACC4 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 223 2e-60
MIAB_STAS1 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 223 2e-60
MIAB_STAAT RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 223 2e-60
MIAB_STAAE RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 223 2e-60
MIAB_STAAC RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 223 2e-60
MIAB_STAA8 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 223 2e-60
MIAB_STAA3 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 223 2e-60
MIAB_SYMTH RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 222 5e-60
MIAB_STAAR RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 221 7e-60
MIAB_STAAB RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 221 7e-60
MIAB_BACCR RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 221 7e-60
MIAB_BACC2 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 221 7e-60
MIAB_CALS8 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 221 9e-60
MIAB_MYCPE RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 221 1e-59
MIAB_GEOKA RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 221 1e-59
MIAB_BACHK RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 221 1e-59
MIAB_BACCZ RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 221 1e-59
MIAB_BACC7 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 221 1e-59
MIAB_BACC1 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 221 1e-59
MIAB_BACC0 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 221 1e-59
MIAB_BACAH RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 221 1e-59
MIAB_DESRM RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 220 2e-59
MIAB_METI4 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 220 2e-59
MIAB_OCEIH RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 219 3e-59
MIAB_BACA2 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 219 4e-59
MIAB_AQUAE RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 219 4e-59
MIAB_BACP2 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 219 5e-59
MIAB_FUSNN RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 218 8e-59
MIAB_CLOD6 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 217 1e-58
MIAB_BACAN RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 217 1e-58
MIAB_SYNWW RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 216 4e-58
MIAB_RICBR RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 216 4e-58
MIAB_RICB8 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 215 5e-58
MIAB_GEOUR RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 215 5e-58
MIAB_THEYD RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 215 7e-58
MIAB_THEAB RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 215 7e-58
MIAB_CAUSK RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 215 7e-58
MIAB_ALKMQ RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 215 7e-58
MIAB_MOOTA RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 214 9e-58
MIAB_CYAP8 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 214 9e-58
MIAB_SULSY RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 214 1e-57
MIAB_THESQ RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 214 1e-57
MIAB_THEP1 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 214 1e-57
MIAB_PARUW RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 214 1e-57
MIAB_HYDS0 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 213 2e-57
MIAB_THEMA RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 213 3e-57
MIAB_RHIL3 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 213 3e-57
MIAB_GEOTN RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 213 3e-57
MIAB_BRASO RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 213 3e-57
MIAB_ANOFW RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 213 3e-57
MIAB_EHRRW RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 212 4e-57
MIAB_DESHY RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 212 4e-57
MIAB_STAHJ RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 212 6e-57
MIAB_EHRRG RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 212 6e-57
MIAB_RICFE RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 211 7e-57
MIAB_OCHA4 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 211 7e-57
MIAB_SYNJA RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 211 1e-56
MIAB_SYNAS RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 211 1e-56
MIAB_MARMM RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 211 1e-56
MIAB_UNCTG RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 210 2e-56
MIAB_THEM4 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 210 2e-56
MIAB_ANAVT RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 210 2e-56
MIAB_DICT6 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 209 3e-56
MIAB_RICRS RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 209 4e-56
MIAB_RICRO RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 209 4e-56
MIAB_PETMO RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 209 4e-56
MIAB_BACSK RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 209 4e-56
MIAB_ARCB4 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 209 5e-56
MIAB_SYNP2 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 208 6e-56
MIAB_SYNJB RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 208 6e-56
MIAB_SYNFM RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 208 6e-56
MIAB_SILST RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 208 6e-56
MIAB_ANAPZ RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 208 6e-56
MIAB_RICCN RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 208 8e-56
MIAB_BURPP RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 208 8e-56
MIAB_RHIME RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 207 1e-55
MIAB_EHRCJ RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 207 1e-55
MIAB_DESPS RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 207 1e-55
MIAB_DECAR RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 207 1e-55
MIAB_BUCBP RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 207 1e-55
MIAB_BURXL RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 207 2e-55
MIAB_RICAH RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 206 2e-55
MIAB_ROSDO RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 206 3e-55
MIAB_RICCK RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 206 3e-55
MIAB_FERNB RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 206 3e-55
MIAB_SALTO RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 206 4e-55
MIAB_BRASB RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 206 4e-55
MIAB_SINMW RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 205 5e-55
MIAB_PROMS RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 204 9e-55
MIAB_COPPD RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 204 9e-55
MIAB_THEFY RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 204 1e-54
MIAB_RHOP5 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 204 1e-54
MIAB_PROMM RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 204 1e-54
MIAB_PARL1 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 204 1e-54
MIAB_EHRCR RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 204 1e-54
MIAB_BURS3 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 204 1e-54
MIAB_BACSU RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 204 1e-54
MIAB_BACLD RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 204 1e-54
MIAB_RICM5 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 204 2e-54
MIAB_RHIEC RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 204 2e-54
MIAB_PROM3 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 204 2e-54
MIAB_METFK RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 204 2e-54
MIAB_DESOH RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 204 2e-54
MIAB_COREF RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 204 2e-54
MIAB_RHIE6 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 203 2e-54
MIAB_ALHEH RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 203 2e-54
MIAB_TRIEI RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 203 3e-54
MIAB_RALSO RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 203 3e-54
MIAB_LEPBP RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 203 3e-54
MIAB_LEPBA RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 203 3e-54
MIAB_DESDG RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 203 3e-54
MIAB_BURM1 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 202 3e-54
MIAB_CYAA5 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 202 4e-54
MIAB_CLAM3 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 202 4e-54
MIAB_BURTA RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 202 4e-54
MIAB_SILPO RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 202 6e-54
MIAB_BURA4 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 202 6e-54
MIAB_BARQU RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 202 6e-54
MIAB_ACTP7 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 202 6e-54
MIAB_ACTP2 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 202 6e-54
MIAB_SYNR3 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 201 8e-54
MIAB_RHOS1 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 201 8e-54
MIAB_BURCM RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 201 8e-54
MIAB_BRAJA RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 201 8e-54
MIAB_BART1 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 201 8e-54
MIAB_RHILW RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 201 1e-53
MIAB_HAES2 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 201 1e-53
MIAB_HAES1 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 201 1e-53
MIAB_CORDI RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 201 1e-53
MIAB_ACTPJ RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 201 1e-53
MIAB_METEP RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 200 2e-53
MIAB_BURP8 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 200 2e-53
MIAB_BURCJ RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 200 2e-53
MIAB_BURCH RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 200 2e-53
MIAB_BURCC RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 200 2e-53
MIAB_BURCA RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 200 2e-53
MIAB_BACHD RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 200 2e-53
MIAB_ANASP RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 200 2e-53
MIAB_SALPK RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 200 2e-53
MIAB_SALPA RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 200 2e-53
MIAB_SACEN RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 200 2e-53
MIAB_NEIM0 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 200 2e-53
MIAB_LYSSC RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 200 2e-53
MIAB_BURVG RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 200 2e-53
MIAB_BURPS RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 200 2e-53
MIAB_BURP6 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 200 2e-53
MIAB_BURP0 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 200 2e-53
MIAB_BURMS RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 200 2e-53
MIAB_BURMA RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 200 2e-53
MIAB_BURM9 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 200 2e-53
MIAB_BURM7 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 200 2e-53
MIAB_BRUSU RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 200 2e-53
MIAB_BRUO2 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 200 2e-53
MIAB_BRUC2 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 200 2e-53
MIAB_BRUAB RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 200 2e-53
MIAB_BRUA2 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 200 2e-53
MIAB_BRUA1 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 200 2e-53
MIAB_THELT RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 199 3e-53
MIAB_SALAR RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 199 3e-53
MIAB_PROMA RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 199 3e-53
MIAB_JANMA RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 199 3e-53
MIAB_GLOVI RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 199 3e-53
MIAB_CYAP7 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 199 3e-53
MIAB_CORGL RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 199 3e-53
MIAB_CHRVO RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 199 3e-53
MIAB_BRUSI RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 199 3e-53
MIAB_SALTY RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 199 4e-53
MIAB_SALTI RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 199 4e-53
MIAB_SALSV RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 199 4e-53
MIAB_SALPB RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 199 4e-53
MIAB_SALNS RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 199 4e-53
MIAB_SALHS RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 199 4e-53
MIAB_SALG2 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 199 4e-53
MIAB_SALEP RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 199 4e-53
MIAB_SALDC RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 199 4e-53
MIAB_SALCH RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 199 4e-53
MIAB_SALA4 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 199 4e-53
MIAB_NEIG2 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 199 4e-53
MIAB_HAEIN RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 199 4e-53
MIAB_HAEIG RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 199 4e-53
MIAB_CORJK RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 199 4e-53
MIAB_CORGB RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 199 4e-53
MIAB_BORA1 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 199 4e-53
MIAB_RHOS4 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 199 5e-53
MIAB_ORITB RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 199 5e-53
MIAB_ECO81 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 199 5e-53
MIAB_CLAMS RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 199 5e-53
MIAB_STAES RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 198 6e-53
MIAB_STAEQ RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 198 6e-53
MIAB_NITHX RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 198 6e-53
MIAB_NEIMF RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 198 6e-53
MIAB_NEIG1 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 198 6e-53
MIAB_BARBK RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 198 6e-53
MIAB_ARTAT RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 198 6e-53
MIAB_PROM0 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 198 8e-53
MIAB_NOSP7 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 198 8e-53
MIAB_MESSB RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 198 8e-53
MIAB_HAEIE RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 198 8e-53
MIAB_HAEI8 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 198 8e-53
MIAB_BURP1 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 198 8e-53
MIAB_BRUME RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 198 8e-53
MIAB_BARHE RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 198 8e-53
MIAB_SHISS RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 197 1e-52
MIAB_SHIBS RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 197 1e-52
MIAB_RICTY RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 197 1e-52
MIAB_NEIMB RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 197 1e-52
MIAB_HAEDU RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 197 1e-52
MIAB_CITK8 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 197 1e-52
MIAB_SHIFL RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 197 1e-52
MIAB_SHIF8 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 197 1e-52
MIAB_SHIDS RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 197 1e-52
MIAB_RHOPA RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 197 1e-52
MIAB_NITMU RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 197 1e-52
MIAB_KLEP3 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 197 1e-52
MIAB_ESCF3 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 197 1e-52
MIAB_ECOUT RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 197 1e-52
MIAB_ECOSM RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 197 1e-52
MIAB_ECOSE RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 197 1e-52
MIAB_ECOLU RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 197 1e-52
MIAB_ECOLI RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 197 1e-52
MIAB_ECOLC RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 197 1e-52
MIAB_ECOL6 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 197 1e-52
MIAB_ECOL5 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 197 1e-52
MIAB_ECOK1 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 197 1e-52
MIAB_ECOHS RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 197 1e-52
MIAB_ECODH RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 197 1e-52
MIAB_ECO8A RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 197 1e-52
MIAB_ECO7I RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 197 1e-52
MIAB_ECO5E RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 197 1e-52
MIAB_ECO57 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 197 1e-52
MIAB_ECO55 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 197 1e-52
MIAB_ECO45 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 197 1e-52
MIAB_ECO24 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 197 1e-52
MIAB_POLNS RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 197 2e-52
MIAB_PELCD RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 197 2e-52
MIAB_PROM2 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 196 2e-52
MIAB_PROAC RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 196 2e-52
MIAB_HERAR RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 196 2e-52
MIAB_DELAS RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 196 2e-52
MIAB_ACISJ RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 196 2e-52
MIAB_SULDN RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 196 3e-52
MIAB_POLSQ RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 196 3e-52
MIAB_JANSC RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 196 3e-52
MIAB_RICPR RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 196 4e-52
MIAB_NEIMA RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 196 4e-52
MIAB_MYCA9 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 196 4e-52
MIAB_KOCRD RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 196 4e-52
MIAB_KLEP7 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 196 4e-52
MIAB_ENT38 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 196 4e-52
MIAB_ANAMM RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 196 4e-52
MIAB_SHIB3 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 195 5e-52
MIAB_AGRT5 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 195 5e-52
MIAB_YERPN RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 195 7e-52
MIAB_YERPE RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 195 7e-52
MIAB_YERPA RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 195 7e-52
MIAB_RHOPT RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 195 7e-52
MIAB_PROM5 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 195 7e-52
MIAB_BIFAA RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 195 7e-52
MIAB_TROWT RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 194 9e-52
MIAB_THIDA RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 194 9e-52
MIAB_PASMU RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 194 1e-51
MIAB_ORITI RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 194 1e-51
MIAB_NOCSJ RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 194 1e-51
MIAB_ENTS8 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 194 1e-51
MIAB_YERPY RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 194 2e-51
MIAB_TROW8 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 194 2e-51
MIAB_DESVV RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 194 2e-51
MIAB_MYCGI RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 193 2e-51
MIAB_DESVH RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 193 2e-51
MIAB_YERPP RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 193 3e-51
MIAB_YERPG RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 193 3e-51
MIAB_WOLPP RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 193 3e-51
MIAB1_MYCMM RecName: Full=(Dimethylallyl)adenosine tRNA methylth... 193 3e-51
Y416_RICPR RecName: Full=Putative methylthiotransferase RP416; ... 192 4e-51
MIAB_YERPS RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 192 4e-51
MIAB_YERPB RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 192 4e-51
MIAB_YERP3 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 192 4e-51
MIAB_SPHAL RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 192 4e-51
MIAB_ACTSZ RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 192 4e-51
RIMO_DESHY RecName: Full=Ribosomal protein S12 methylthiotransfe... 192 5e-51
MIAB_STRCO RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 192 5e-51
MIAB_RHILO RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 192 5e-51
MIAB_PSEA6 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 192 5e-51
MIAB_NITSB RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 192 5e-51
MIAB_LEPBL RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 192 5e-51
MIAB_LEPBJ RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 192 5e-51
MIAB_XANCP RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 192 6e-51
MIAB_XANCB RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 192 6e-51
MIAB_XANC8 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 192 6e-51
MIAB_SHELP RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 192 6e-51
MIAB_RALPJ RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 192 6e-51
MIAB_MANSM RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 192 6e-51
MIAB_LEPCP RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 192 6e-51
MIAB_ACIBL RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 192 6e-51
MIAB_NITWN RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 191 8e-51
MIAB_AZOC5 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 191 8e-51
RIMO_CLOD6 RecName: Full=Ribosomal protein S12 methylthiotransfe... 191 1e-50
MIAB_ZYMMO RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 191 1e-50
MIAB_CORU7 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 191 1e-50
MIAB_XANP2 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 191 1e-50
MIAB_RHOPB RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 191 1e-50
MIAB_PARDP RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 191 1e-50
MIAB_MYCUA RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 191 1e-50
MIAB_MYCS2 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 191 1e-50
MIAB_GEOLS RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 191 1e-50
MIAB_GEOBB RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 191 1e-50
MIAB_ACAM1 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 191 1e-50
MIAB2_MYCMM RecName: Full=(Dimethylallyl)adenosine tRNA methylth... 191 1e-50
MIAB_YERE8 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 190 2e-50
MIAB_SHESH RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 190 2e-50
MIAB_SHEAM RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 190 2e-50
MIAB_RHOS5 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 190 2e-50
MIAB_PROMP RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 190 2e-50
MIAB_PHOLL RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 190 2e-50
MIAB_NITEC RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 190 2e-50
MIAB_COLP3 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 190 2e-50
MIAB_PSYWF RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 190 2e-50
MIAB_PARD8 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 190 2e-50
MIAB_SYNY3 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 189 3e-50
MIAB_LEPIN RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 189 3e-50
MIAB_LEPIC RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 189 3e-50
MIAB_GRAFK RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 189 3e-50
MIAB_RHOBA RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 189 4e-50
MIAB_LAWIP RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 189 4e-50
MIAB_STRAW RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 189 5e-50
MIAB_RENSM RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 189 5e-50
MIAB_CAMFF RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 189 5e-50
MIAB_PSESM RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 188 7e-50
MIAB_SERP5 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 188 9e-50
MIAB_SALAI RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 188 9e-50
MIAB_RALEH RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 188 9e-50
MIAB_MYCTU RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 188 9e-50
MIAB_MYCTA RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 188 9e-50
MIAB_MYCBP RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 188 9e-50
MIAB_MYCBO RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 188 9e-50
MIAB_MICAN RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 188 9e-50
MIAB_LEGPL RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 188 9e-50
MIAB_LEGPH RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 188 9e-50
MIAB_LEGPC RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 188 9e-50
MIAB_LEGPA RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 188 9e-50
MIAB_DINSH RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 188 9e-50
MIAB_CUPTR RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 188 9e-50
MIAB_SODGM RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 187 1e-49
MIAB_SHEWM RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 187 1e-49
MIAB_SHEHH RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 187 1e-49
MIAB_RHOFD RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 187 1e-49
MIAB_PSEU2 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 187 1e-49
MIAB_POLNA RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 187 1e-49
MIAB_NOCFA RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 187 1e-49
MIAB_WOLTR RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 187 1e-49
MIAB_MARMS RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 187 2e-49
MIAB_HYPNA RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 187 2e-49
MIAB_ERWCT RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 187 2e-49
MIAB_ACIAC RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 187 2e-49
MIAB_WOLSU RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 186 3e-49
MIAB_VIBVU RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 186 3e-49
MIAB_STRMK RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 186 3e-49
MIAB_SHESA RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 186 3e-49
MIAB_PSE14 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 186 3e-49
MIAB_PROMH RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 186 3e-49
MIAB_NEOSM RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 186 3e-49
MIAB_GEOMG RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 186 3e-49
MIAB_VIBVY RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 186 3e-49
MIAB_STRM5 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 186 3e-49
MIAB_SHEPA RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 186 3e-49
MIAB_METPP RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 186 3e-49
MIAB_HELP2 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 186 3e-49
MIAB_SHESR RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 186 4e-49
MIAB_ERYLH RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 186 4e-49
MIAB_WOLPM RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 185 6e-49
MIAB_PROM4 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 185 6e-49
MIAB_GLUDA RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 185 6e-49
MIAB_THET8 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 185 7e-49
MIAB_THET2 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 185 7e-49
MIAB_SHEFN RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 185 7e-49
MIAB_RHOPS RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 185 7e-49
MIAB_HELPJ RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 185 7e-49
MIAB_SHEON RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 184 1e-48
MIAB_POLSJ RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 184 1e-48
MIAB_ALTMD RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 184 1e-48
MIAB_SHESM RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 184 1e-48
MIAB_RHOP2 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 184 1e-48
MIAB_HELPH RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 184 1e-48
MIAB_STRGG RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 184 2e-48
MIAB_HELAH RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 184 2e-48
MIAB_AERS4 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 184 2e-48
MIAB_SHEDO RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 183 2e-48
MIAB_RALME RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 183 2e-48
MIAB_RALEJ RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 183 2e-48
MIAB_BUCAI RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 183 2e-48
MIAB_ACIC1 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 183 2e-48
RIMO_ALKMQ RecName: Full=Ribosomal protein S12 methylthiotransfe... 183 3e-48
MIAB_VIBCH RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 183 3e-48
MIAB_VIBC3 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 183 3e-48
MIAB_HELPY RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 183 3e-48
MIAB_ARTS2 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 183 3e-48
MIAB_VIBPA RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 182 4e-48
MIAB_SACD2 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 182 4e-48
MIAB_PROM9 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 182 4e-48
MIAB_PELUB RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 182 4e-48
MIAB_ERWT9 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 182 4e-48
MIAB_COXBU RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 182 4e-48
MIAB_COXBR RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 182 4e-48
MIAB_COXB2 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 182 4e-48
MIAB_COXB1 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 182 4e-48
MIAB_AERHH RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 182 4e-48
MIAB_PSEPF RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 182 5e-48
MIAB_LEIXX RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 182 5e-48
MIAB_HELPG RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 182 5e-48
MIAB_COXBN RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 182 5e-48
MIAB_BORPA RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 182 6e-48
MIAB_SYNS3 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 181 8e-48
MIAB_AZOSB RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 181 8e-48
RIMO_CLOPE RecName: Full=Ribosomal protein S12 methylthiotransfe... 181 1e-47
RIMO_CLOP1 RecName: Full=Ribosomal protein S12 methylthiotransfe... 181 1e-47
MIAB_PSEF5 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 181 1e-47
MIAB_NITOC RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 181 1e-47
MIAB_MYCSS RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 181 1e-47
MIAB_MYCSK RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 181 1e-47
MIAB_HERA2 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 181 1e-47
MIAB_GEOSL RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 181 1e-47
RIMO_CLOPS RecName: Full=Ribosomal protein S12 methylthiotransfe... 181 1e-47
MIAB_VIBHB RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 181 1e-47
MIAB_SHEB9 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 181 1e-47
MIAB_SHEB8 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 181 1e-47
MIAB_OLICO RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 181 1e-47
MIAB_MYCSJ RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 181 1e-47
MIAB_BAUCH RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 181 1e-47
MIAB_SULNB RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 180 2e-47
MIAB_SHEB5 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 180 2e-47
MIAB_NITEU RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 180 2e-47
MIAB_MYCVP RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 180 2e-47
RIMO_CLOPH RecName: Full=Ribosomal protein S12 methylthiotransfe... 180 2e-47
MIAB_HELPS RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 180 2e-47
MIAB_ACICJ RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 180 2e-47
MIAB_PSEMY RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 179 3e-47
MIAB_FRATW RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 179 3e-47
MIAB_FRATT RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 179 3e-47
MIAB_FRATN RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 179 3e-47
MIAB_FRATM RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 179 3e-47
MIAB_FRAT1 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 179 3e-47
RIMO_CLOK5 RecName: Full=Ribosomal protein S12 methylthiotransfe... 179 4e-47
MIAB_THICR RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 179 4e-47
MIAB_RHOSR RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 179 4e-47
MIAB_HAHCH RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 179 4e-47
MIAB_CAMC5 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 179 4e-47
RIMO_CLOTE RecName: Full=Ribosomal protein S12 methylthiotransfe... 179 5e-47
MIAB_SOLUE RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 179 5e-47
MIAB_SHESW RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 179 5e-47
MIAB_SHEPC RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 179 5e-47
MIAB_OPITP RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 179 5e-47
MIAB_FRAP2 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 179 5e-47
MIAB_AZOSE RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 179 5e-47
MIAB_VIBFM RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 178 7e-47
MIAB_VIBF1 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 178 7e-47
MIAB_CHRSD RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 178 7e-47
MIAB_PSEHT RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 178 9e-47
MIAB_PELPD RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 178 9e-47
MIAB_SYNPX RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 177 1e-46
MIAB_PSYCK RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 177 1e-46
MIAB_PSEU5 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 177 1e-46
MIAB_MYCLE RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 177 1e-46
RIMO_PROMS RecName: Full=Ribosomal protein S12 methylthiotransfe... 177 2e-46
RIMO_PROM2 RecName: Full=Ribosomal protein S12 methylthiotransfe... 177 2e-46
MIAB_PHOPR RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 177 2e-46
MIAB_PHEZH RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 177 2e-46
MIAB_PSYIN RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 177 2e-46
RIMO_PROM0 RecName: Full=Ribosomal protein S12 methylthiotransfe... 176 3e-46
MIAB_MAGSA RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 176 3e-46
MIAB_PSEAE RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 176 3e-46
MIAB_PSEAB RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 176 3e-46
MIAB_KINRD RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 176 3e-46
RIMO_CLOBA RecName: Full=Ribosomal protein S12 methylthiotransfe... 176 4e-46
MIAB_PSEA7 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 176 4e-46
MIAB_DEIRA RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 176 4e-46
MIAB_AZOPC RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 176 4e-46
MIAB_SYNP6 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 175 8e-46
MIAB_SYNE7 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 175 8e-46
MIAB_ALISL RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 175 8e-46
RIMO_SYNS9 RecName: Full=Ribosomal protein S12 methylthiotransfe... 174 1e-45
MIAB_SYNS9 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 174 1e-45
MIAB_HALHL RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 174 1e-45
MIAB_FRATO RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 174 1e-45
MIAB_FRATH RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 174 1e-45
MIAB_FRATF RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 174 1e-45
MIAB_CAMC1 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 174 1e-45
MIAB_ACIBS RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 174 1e-45
RIMO_THETN RecName: Full=Ribosomal protein S12 methylthiotransfe... 174 1e-45
RIMO_PROM4 RecName: Full=Ribosomal protein S12 methylthiotransfe... 174 2e-45
RIMO_BDEBA RecName: Full=Ribosomal protein S12 methylthiotransfe... 174 2e-45
MIAB_PSYA2 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 174 2e-45
MIAB_IDILO RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 174 2e-45
MIAB_BUCAP RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 174 2e-45
MIAB_BDEBA RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 174 2e-45
MIAB_ACIBY RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 174 2e-45
MIAB_ACIBC RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 174 2e-45
MIAB_ACIB5 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 174 2e-45
MIAB_ACIB3 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 174 2e-45
RIMO_THEPX RecName: Full=Ribosomal protein S12 methylthiotransfe... 173 2e-45
MIAB_GLUOX RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 173 2e-45
MIAB_BIFLI RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 173 2e-45
MIAB_ACIAD RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 173 2e-45
RIMO_PELTS RecName: Full=Ribosomal protein S12 methylthiotransfe... 173 3e-45
MIAB_XANOR RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 173 3e-45
MIAB_TREDE RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 173 3e-45
MIAB_CHLT3 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 173 3e-45
RIMO_PROMP RecName: Full=Ribosomal protein S12 methylthiotransfe... 172 4e-45
MIAB_XANC5 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 172 4e-45
MIAB_XANAC RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 172 4e-45
MIAB_SYNSC RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 172 4e-45
MIAB_METCA RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 172 4e-45
MIAB_CHLCH RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 172 4e-45
MIAB_BACTN RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 172 4e-45
RIMO_THEP3 RecName: Full=Ribosomal protein S12 methylthiotransfe... 172 5e-45
RIMO_PROM9 RecName: Full=Ribosomal protein S12 methylthiotransfe... 172 5e-45
MIAB_CAMJ8 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 172 5e-45
MIAB_BIFLO RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 172 5e-45
MIAB_BIFLD RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 172 5e-45
Y867_METJA RecName: Full=Putative methylthiotransferase MJ0867; ... 172 6e-45
MIAB_ROSS1 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 172 6e-45
MIAB_BEII9 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 172 6e-45
MIAB_ACIF5 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 172 6e-45
MIAB_ACIF2 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 172 6e-45
RIMO_GEOSL RecName: Full=Ribosomal protein S12 methylthiotransfe... 171 8e-45
RIMO_CLONN RecName: Full=Ribosomal protein S12 methylthiotransfe... 171 8e-45
MIAB_CELJU RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 171 8e-45
Y285_HELPY RecName: Full=Putative methylthiotransferase HP_0285;... 171 1e-44
MIAB_XANOP RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 171 1e-44
MIAB_XANOM RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 171 1e-44
MIAB_THEEB RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 171 1e-44
MIAB_SPHWW RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 171 1e-44
MIAB_GRABC RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 171 1e-44
MIAB_FRAAA RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 171 1e-44
MIAB_CAMJJ RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 171 1e-44
MIAB_CAMJE RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 171 1e-44
Y285_HELPJ RecName: Full=Putative methylthiotransferase jhp_0270... 171 1e-44
RIMO_TRIEI RecName: Full=Ribosomal protein S12 methylthiotransfe... 171 1e-44
RIMO_DESRM RecName: Full=Ribosomal protein S12 methylthiotransfe... 171 1e-44
RIMO_ALKOO RecName: Full=Ribosomal protein S12 methylthiotransfe... 171 1e-44
MIAB_VEREI RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 171 1e-44
MIAB_CAMJD RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 171 1e-44
MIAB_METS4 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 170 2e-44
MIAB_METPB RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 170 2e-44
MIAB_BACV8 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 170 2e-44
MIAB_BACFR RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 170 2e-44
MIAB_BACFN RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 170 2e-44
MIAB_ANADF RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 170 2e-44
RIMO_CLOTH RecName: Full=Ribosomal protein S12 methylthiotransfe... 170 2e-44
RIMO_CLOBB RecName: Full=Ribosomal protein S12 methylthiotransfe... 170 2e-44
MIAB_PROM1 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 170 2e-44
MIAB_METC4 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 170 2e-44
MIAB_FLAJ1 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 170 2e-44
MIAB_CHLTE RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 170 2e-44
MIAB_NOVAD RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 169 3e-44
MIAB_MYCA1 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 169 3e-44
MIAB_MAGSM RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 169 3e-44
MIAB_FRASC RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 169 3e-44
MIAB_FLAPJ RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 169 3e-44
MIAB_CAMJR RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 169 3e-44
MIAB_BORBR RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 169 3e-44
MIAB_SYNPW RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 169 4e-44
RIMO_RHOBA RecName: Full=Ribosomal protein S12 methylthiotransfe... 168 7e-44
MIAB_MYCPA RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 168 7e-44
MIAB_DEHE1 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 168 7e-44
MIAB_BORPE RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 168 7e-44
Y1729_PYRAB RecName: Full=Putative methylthiotransferase PYRAB17... 168 9e-44
MIAB_PROMT RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 168 9e-44
MIAB_FRASN RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 168 9e-44
RIMO_THELT RecName: Full=Ribosomal protein S12 methylthiotransfe... 167 1e-43
RIMO_SYNPW RecName: Full=Ribosomal protein S12 methylthiotransfe... 167 2e-43
MIAB_MYXXD RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 167 2e-43
RIMO_ANAVT RecName: Full=Ribosomal protein S12 methylthiotransfe... 167 2e-43
RIMO_SYNPX RecName: Full=Ribosomal protein S12 methylthiotransfe... 166 3e-43
MIAB_RHOCS RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 166 3e-43
MIAB_CHLP8 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 166 3e-43
MIAB_CYTH3 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 166 4e-43
MIAB_ANASK RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 166 4e-43
RIMO_GEOUR RecName: Full=Ribosomal protein S12 methylthiotransfe... 166 5e-43
RIMO_PROM5 RecName: Full=Ribosomal protein S12 methylthiotransfe... 165 6e-43
RIMO_PROM3 RecName: Full=Ribosomal protein S12 methylthiotransfe... 165 6e-43
RIMO_CALS8 RecName: Full=Ribosomal protein S12 methylthiotransfe... 165 6e-43
MIAB_PROVI RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 165 6e-43
MIAB_MARAV RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 165 6e-43
RIMO_ANASP RecName: Full=Ribosomal protein S12 methylthiotransfe... 165 8e-43
MIAB_DICNV RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 165 8e-43
MIAB_DEHSB RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 165 8e-43
RIMO_SYNSC RecName: Full=Ribosomal protein S12 methylthiotransfe... 164 1e-42
RIMO_MOOTA RecName: Full=Ribosomal protein S12 methylthiotransfe... 164 1e-42
MIAB_DEHSC RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 164 1e-42
RIMO_CLOAB RecName: Full=Ribosomal protein S12 methylthiotransfe... 164 2e-42
MIAB_PORGI RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 164 2e-42
MIAB_METRJ RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 164 2e-42
MIAB_BORPD RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 164 2e-42
RIMO_MICAN RecName: Full=Ribosomal protein S12 methylthiotransfe... 163 3e-42
RIMO_GEOMG RecName: Full=Ribosomal protein S12 methylthiotransfe... 163 3e-42
RIMO_DICT6 RecName: Full=Ribosomal protein S12 methylthiotransfe... 163 3e-42
RIMO_CYAP7 RecName: Full=Ribosomal protein S12 methylthiotransfe... 163 3e-42
RIMO_SYNY3 RecName: Full=Ribosomal protein S12 methylthiotransfe... 162 5e-42
RIMO_SYNS3 RecName: Full=Ribosomal protein S12 methylthiotransfe... 162 5e-42
RIMO_NATTJ RecName: Full=Ribosomal protein S12 methylthiotransfe... 162 7e-42
MIAB_DEIGD RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 162 7e-42
MIAB_TREPS RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 161 9e-42
MIAB_TREPA RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 161 9e-42
RIMO_PROMM RecName: Full=Ribosomal protein S12 methylthiotransfe... 161 1e-41
RIMO_CLOB8 RecName: Full=Ribosomal protein S12 methylthiotransfe... 161 1e-41
MIAB_PORG3 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 161 1e-41
MIAB_CAMHC RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 161 1e-41
MIAB_PELPB RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 160 1e-41
MIAB_ANADE RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 160 2e-41
RIMO_SYNP2 RecName: Full=Ribosomal protein S12 methylthiotransfe... 160 3e-41
MIAB_SALRD RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 160 3e-41
RIMO_SACEN RecName: Full=Ribosomal protein S12 methylthiotransfe... 159 4e-41
RIMO_NOSP7 RecName: Full=Ribosomal protein S12 methylthiotransfe... 159 4e-41
RIMO_SYMTH RecName: Full=Ribosomal protein S12 methylthiotransfe... 159 6e-41
RIMO_CYAA5 RecName: Full=Ribosomal protein S12 methylthiotransfe... 158 7e-41
MIAB_RHORT RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 158 7e-41
RIMO_SYNE7 RecName: Full=Ribosomal protein S12 methylthiotransfe... 158 1e-40
MIAB_CHLAA RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 158 1e-40
RIMO_SYNP6 RecName: Full=Ribosomal protein S12 methylthiotransfe... 157 1e-40
RIMO_GEOBB RecName: Full=Ribosomal protein S12 methylthiotransfe... 157 1e-40
MIAB_VESOH RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 157 1e-40
MIAB_AMOA5 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 157 1e-40
RIMO_CYAP8 RecName: Full=Ribosomal protein S12 methylthiotransfe... 157 2e-40
RIMO_CARHZ RecName: Full=Ribosomal protein S12 methylthiotransfe... 157 2e-40
RIMO_PROMA RecName: Full=Ribosomal protein S12 methylthiotransfe... 157 2e-40
RIMO_LEPBP RecName: Full=Ribosomal protein S12 methylthiotransfe... 157 2e-40
RIMO_LEPBA RecName: Full=Ribosomal protein S12 methylthiotransfe... 157 2e-40
RIMO_SYNJB RecName: Full=Ribosomal protein S12 methylthiotransfe... 156 4e-40
RIMO_HELMI RecName: Full=Ribosomal protein S12 methylthiotransfe... 156 4e-40
RIMO_CLOBM RecName: Full=Ribosomal protein S12 methylthiotransfe... 156 4e-40
RIMO_MAGSM RecName: Full=Ribosomal protein S12 methylthiotransfe... 155 5e-40
RIMO_CLOBL RecName: Full=Ribosomal protein S12 methylthiotransfe... 155 5e-40
RIMO_LEPBL RecName: Full=Ribosomal protein S12 methylthiotransfe... 155 6e-40
MIAB_XYLFT RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 155 6e-40
MIAB_XYLF2 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 155 6e-40
RIMO_CLOBH RecName: Full=Ribosomal protein S12 methylthiotransfe... 155 8e-40
RIMO_CLOB1 RecName: Full=Ribosomal protein S12 methylthiotransfe... 155 8e-40
MIAB_CHLPD RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 155 8e-40
MIAB_CHLPB RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 155 8e-40
Y1875_PYRHO RecName: Full=Putative methylthiotransferase PH1875;... 154 1e-39
RIMO_HAHCH RecName: Full=Ribosomal protein S12 methylthiotransfe... 154 1e-39
RIMO_CLOBK RecName: Full=Ribosomal protein S12 methylthiotransfe... 154 1e-39
RIMO_AQUAE RecName: Full=Ribosomal protein S12 methylthiotransfe... 154 1e-39
MIAB_XYLFM RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 154 1e-39
MIAB_PROA2 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 154 1e-39
RIMO_GEOLS RecName: Full=Ribosomal protein S12 methylthiotransfe... 154 1e-39
RIMO_PELCD RecName: Full=Ribosomal protein S12 methylthiotransfe... 154 2e-39
RIMO_LEPIN RecName: Full=Ribosomal protein S12 methylthiotransfe... 154 2e-39
RIMO_CHLAA RecName: Full=Ribosomal protein S12 methylthiotransfe... 154 2e-39
RIMO_ACAM1 RecName: Full=Ribosomal protein S12 methylthiotransfe... 153 2e-39
RIMO_KLEP7 RecName: Full=Ribosomal protein S12 methylthiotransfe... 153 3e-39
RIMO_KLEP3 RecName: Full=Ribosomal protein S12 methylthiotransfe... 153 3e-39
RIMO_LEPBJ RecName: Full=Ribosomal protein S12 methylthiotransfe... 152 4e-39
MIAB_PELLD RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 152 4e-39
RIMO_SYNR3 RecName: Full=Ribosomal protein S12 methylthiotransfe... 152 5e-39
RIMO_GLOVI RecName: Full=Ribosomal protein S12 methylthiotransfe... 152 5e-39
MIAB_CHLL2 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 152 5e-39
RIMO_LEPIC RecName: Full=Ribosomal protein S12 methylthiotransfe... 152 7e-39
RIMO_FERNB RecName: Full=Ribosomal protein S12 methylthiotransfe... 151 9e-39
RIMO_CYTH3 RecName: Full=Ribosomal protein S12 methylthiotransfe... 151 9e-39
RIMO_AERS4 RecName: Full=Ribosomal protein S12 methylthiotransfe... 151 1e-38
MIAB_XYLFA RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 151 1e-38
CDKAL_XENTR RecName: Full=CDK5 regulatory subunit-associated pro... 151 1e-38
RIMO_SYNJA RecName: Full=Ribosomal protein S12 methylthiotransfe... 150 2e-38
Y826_METTH RecName: Full=Putative methylthiotransferase MTH_826;... 149 3e-38
RIMO_PROMH RecName: Full=Ribosomal protein S12 methylthiotransfe... 149 3e-38
RIMO_CITK8 RecName: Full=Ribosomal protein S12 methylthiotransfe... 149 3e-38
CDKAL_DANRE RecName: Full=CDK5 regulatory subunit-associated pro... 149 3e-38
RIMO_SYNWW RecName: Full=Ribosomal protein S12 methylthiotransfe... 149 4e-38
RIMO_AERHH RecName: Full=Ribosomal protein S12 methylthiotransfe... 149 4e-38
MIAB_RUTMC RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 149 4e-38
MIAB_ALCBS RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 149 4e-38
CDKAL_XENLA RecName: Full=CDK5 regulatory subunit-associated pro... 149 4e-38
RIMO_PARUW RecName: Full=Ribosomal protein S12 methylthiotransfe... 149 6e-38
RIMO_THESQ RecName: Full=Ribosomal protein S12 methylthiotransfe... 148 8e-38
RIMO_THEMA RecName: Full=Ribosomal protein S12 methylthiotransfe... 148 8e-38
RIMO_THEP1 RecName: Full=Ribosomal protein S12 methylthiotransfe... 148 1e-37
RIMO_VIBVY RecName: Full=Ribosomal protein S12 methylthiotransfe... 147 1e-37
RIMO_THEYD RecName: Full=Ribosomal protein S12 methylthiotransfe... 147 1e-37
RIMO_PELPD RecName: Full=Ribosomal protein S12 methylthiotransfe... 147 1e-37
RIMO_ESCF3 RecName: Full=Ribosomal protein S12 methylthiotransfe... 147 2e-37
RIMO_PETMO RecName: Full=Ribosomal protein S12 methylthiotransfe... 147 2e-37
CDKAL_DROPS RecName: Full=CDKAL1-like protein; 147 2e-37
RIMO_THEM4 RecName: Full=Ribosomal protein S12 methylthiotransfe... 146 3e-37
RIMO_HERA2 RecName: Full=Ribosomal protein S12 methylthiotransfe... 146 3e-37
RIMO_BACFR RecName: Full=Ribosomal protein S12 methylthiotransfe... 146 3e-37
RIMO_BACFN RecName: Full=Ribosomal protein S12 methylthiotransfe... 146 3e-37
RIMO_STRCO RecName: Full=Ribosomal protein S12 methylthiotransfe... 146 4e-37
RIMO_OPITP RecName: Full=Ribosomal protein S12 methylthiotransfe... 146 4e-37
RIMO_WOLSU RecName: Full=Ribosomal protein S12 methylthiotransfe... 145 5e-37
RIMO_VIBVU RecName: Full=Ribosomal protein S12 methylthiotransfe... 145 5e-37
RIMO_GRAFK RecName: Full=Ribosomal protein S12 methylthiotransfe... 145 5e-37
RIMO_FLAPJ RecName: Full=Ribosomal protein S12 methylthiotransfe... 145 5e-37
CDKAL_HUMAN RecName: Full=CDK5 regulatory subunit-associated pro... 145 5e-37
RIMO_SALPB RecName: Full=Ribosomal protein S12 methylthiotransfe... 145 6e-37
CK5P1_DICDI RecName: Full=CDK5RAP1-like protein; 145 6e-37
CDKAL_DROME RecName: Full=CDKAL1-like protein; 145 6e-37
RIMO_THEEB RecName: Full=Ribosomal protein S12 methylthiotransfe... 144 1e-36
RIMO_SALTI RecName: Full=Ribosomal protein S12 methylthiotransfe... 144 1e-36
RIMO_ERWCT RecName: Full=Ribosomal protein S12 methylthiotransfe... 144 1e-36
Y1247_ARCFU RecName: Full=Putative methylthiotransferase AF_1247... 144 1e-36
RIMO_SHIBS RecName: Full=Ribosomal protein S12 methylthiotransfe... 144 1e-36
RIMO_SHIB3 RecName: Full=Ribosomal protein S12 methylthiotransfe... 144 1e-36
RIMO_ECOSM RecName: Full=Ribosomal protein S12 methylthiotransfe... 144 1e-36
RIMO_ECOSE RecName: Full=Ribosomal protein S12 methylthiotransfe... 144 1e-36
RIMO_ECOLU RecName: Full=Ribosomal protein S12 methylthiotransfe... 144 1e-36
RIMO_ECOLI RecName: Full=Ribosomal protein S12 methylthiotransfe... 144 1e-36
RIMO_ECOLC RecName: Full=Ribosomal protein S12 methylthiotransfe... 144 1e-36
RIMO_ECOHS RecName: Full=Ribosomal protein S12 methylthiotransfe... 144 1e-36
RIMO_ECODH RecName: Full=Ribosomal protein S12 methylthiotransfe... 144 1e-36
RIMO_ECO8A RecName: Full=Ribosomal protein S12 methylthiotransfe... 144 1e-36
RIMO_ECO7I RecName: Full=Ribosomal protein S12 methylthiotransfe... 144 1e-36
RIMO_ECO5E RecName: Full=Ribosomal protein S12 methylthiotransfe... 144 1e-36
RIMO_ECO57 RecName: Full=Ribosomal protein S12 methylthiotransfe... 144 1e-36
RIMO_ECO55 RecName: Full=Ribosomal protein S12 methylthiotransfe... 144 1e-36
RIMO_ECO24 RecName: Full=Ribosomal protein S12 methylthiotransfe... 144 1e-36
RIMO_SALTY RecName: Full=Ribosomal protein S12 methylthiotransfe... 144 2e-36
RIMO_SALSV RecName: Full=Ribosomal protein S12 methylthiotransfe... 144 2e-36
RIMO_SALPK RecName: Full=Ribosomal protein S12 methylthiotransfe... 144 2e-36
RIMO_SALPA RecName: Full=Ribosomal protein S12 methylthiotransfe... 144 2e-36
RIMO_SALNS RecName: Full=Ribosomal protein S12 methylthiotransfe... 144 2e-36
RIMO_SALEP RecName: Full=Ribosomal protein S12 methylthiotransfe... 144 2e-36
RIMO_SALDC RecName: Full=Ribosomal protein S12 methylthiotransfe... 144 2e-36
RIMO_SALCH RecName: Full=Ribosomal protein S12 methylthiotransfe... 144 2e-36
RIMO_SALAR RecName: Full=Ribosomal protein S12 methylthiotransfe... 144 2e-36
RIMO_SALA4 RecName: Full=Ribosomal protein S12 methylthiotransfe... 144 2e-36
RIMO_PSEA6 RecName: Full=Ribosomal protein S12 methylthiotransfe... 144 2e-36
RIMO_PROAC RecName: Full=Ribosomal protein S12 methylthiotransfe... 144 2e-36
RIMO_THICR RecName: Full=Ribosomal protein S12 methylthiotransfe... 143 2e-36
RIMO_ENTS8 RecName: Full=Ribosomal protein S12 methylthiotransfe... 143 2e-36
CDKAL_MOUSE RecName: Full=CDK5 regulatory subunit-associated pro... 143 2e-36
RIMO_SALHS RecName: Full=Ribosomal protein S12 methylthiotransfe... 143 3e-36
RIMO_SALG2 RecName: Full=Ribosomal protein S12 methylthiotransfe... 143 3e-36
RIMO_ECOUT RecName: Full=Ribosomal protein S12 methylthiotransfe... 143 3e-36
RIMO_ECOL6 RecName: Full=Ribosomal protein S12 methylthiotransfe... 143 3e-36
RIMO_ECOL5 RecName: Full=Ribosomal protein S12 methylthiotransfe... 143 3e-36
RIMO_ECOK1 RecName: Full=Ribosomal protein S12 methylthiotransfe... 143 3e-36
RIMO_ECO81 RecName: Full=Ribosomal protein S12 methylthiotransfe... 143 3e-36
RIMO_ECO45 RecName: Full=Ribosomal protein S12 methylthiotransfe... 143 3e-36
RIMO_VIBCH RecName: Full=Ribosomal protein S12 methylthiotransfe... 142 5e-36
RIMO_SHIFL RecName: Full=Ribosomal protein S12 methylthiotransfe... 142 5e-36
RIMO_SHISS RecName: Full=Ribosomal protein S12 methylthiotransfe... 142 7e-36
RIMO_SHIF8 RecName: Full=Ribosomal protein S12 methylthiotransfe... 142 7e-36
RIMO_BACTN RecName: Full=Ribosomal protein S12 methylthiotransfe... 141 9e-36
RIMO_VIBC3 RecName: Full=Ribosomal protein S12 methylthiotransfe... 141 1e-35
RIMO_THIDA RecName: Full=Ribosomal protein S12 methylthiotransfe... 141 1e-35
RIMO_METI4 RecName: Full=Ribosomal protein S12 methylthiotransfe... 141 1e-35
RIMO_FRAP2 RecName: Full=Ribosomal protein S12 methylthiotransfe... 141 1e-35
RIMO_CHLCV RecName: Full=Ribosomal protein S12 methylthiotransfe... 141 1e-35
RIMO_ACIBL RecName: Full=Ribosomal protein S12 methylthiotransfe... 141 1e-35
RIMO_CHLAB RecName: Full=Ribosomal protein S12 methylthiotransfe... 140 2e-35
RIMO_SULNB RecName: Full=Ribosomal protein S12 methylthiotransfe... 140 2e-35
RIMO_DESPS RecName: Full=Ribosomal protein S12 methylthiotransfe... 140 3e-35
RIMO_ACIBT RecName: Full=Ribosomal protein S12 methylthiotransfe... 140 3e-35
RIMO_ACIBS RecName: Full=Ribosomal protein S12 methylthiotransfe... 140 3e-35
RIMO_ACIBC RecName: Full=Ribosomal protein S12 methylthiotransfe... 140 3e-35
RIMO_ACIAD RecName: Full=Ribosomal protein S12 methylthiotransfe... 140 3e-35
RIMO_SULSY RecName: Full=Ribosomal protein S12 methylthiotransfe... 139 4e-35
RIMO_PSEE4 RecName: Full=Ribosomal protein S12 methylthiotransfe... 139 4e-35
RIMO_DESAP RecName: Full=Ribosomal protein S12 methylthiotransfe... 139 4e-35
CK5P1_RAT RecName: Full=CDK5 regulatory subunit-associated prote... 139 4e-35
RIMO_THET8 RecName: Full=Ribosomal protein S12 methylthiotransfe... 139 5e-35
RIMO_THEAB RecName: Full=Ribosomal protein S12 methylthiotransfe... 139 5e-35
RIMO_NOCSJ RecName: Full=Ribosomal protein S12 methylthiotransfe... 139 5e-35
MIAB_HELHP RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 139 5e-35
CK5P1_MOUSE RecName: Full=CDK5 regulatory subunit-associated pro... 139 5e-35
RIMO_PSEPK RecName: Full=Ribosomal protein S12 methylthiotransfe... 139 6e-35
RIMO_PSEP1 RecName: Full=Ribosomal protein S12 methylthiotransfe... 139 6e-35
RIMO_FRATN RecName: Full=Ribosomal protein S12 methylthiotransfe... 139 6e-35
RIMO_CHLFF RecName: Full=Ribosomal protein S12 methylthiotransfe... 139 6e-35
RIMO_CAMJJ RecName: Full=Ribosomal protein S12 methylthiotransfe... 139 6e-35
RIMO_ANASK RecName: Full=Ribosomal protein S12 methylthiotransfe... 139 6e-35
RIMO_PSEPW RecName: Full=Ribosomal protein S12 methylthiotransfe... 138 8e-35
RIMO_CAMJR RecName: Full=Ribosomal protein S12 methylthiotransfe... 138 8e-35
RIMO_CAMJ8 RecName: Full=Ribosomal protein S12 methylthiotransfe... 138 8e-35
CK5P1_CAEEL RecName: Full=CDK5RAP1-like protein; 138 8e-35
RIMO_SYNAS RecName: Full=Ribosomal protein S12 methylthiotransfe... 138 1e-34
RIMO_FLAJ1 RecName: Full=Ribosomal protein S12 methylthiotransfe... 138 1e-34
RIMO_CAMHC RecName: Full=Ribosomal protein S12 methylthiotransfe... 138 1e-34
RIMO_ACIBY RecName: Full=Ribosomal protein S12 methylthiotransfe... 138 1e-34
RIMO_ACIB3 RecName: Full=Ribosomal protein S12 methylthiotransfe... 138 1e-34
RIMO_PSEPG RecName: Full=Ribosomal protein S12 methylthiotransfe... 137 1e-34
RIMO_CAMJD RecName: Full=Ribosomal protein S12 methylthiotransfe... 137 1e-34
RIMO_ELUMP RecName: Full=Ribosomal protein S12 methylthiotransfe... 137 2e-34
RIMO_CAMJE RecName: Full=Ribosomal protein S12 methylthiotransfe... 137 2e-34
RIMO_STRAW RecName: Full=Ribosomal protein S12 methylthiotransfe... 137 2e-34
RIMO_PASMU RecName: Full=Ribosomal protein S12 methylthiotransfe... 137 2e-34
RIMO_PARD8 RecName: Full=Ribosomal protein S12 methylthiotransfe... 137 2e-34
RIMO_HELHP RecName: Full=Ribosomal protein S12 methylthiotransfe... 137 2e-34
RIMO_ACIB5 RecName: Full=Ribosomal protein S12 methylthiotransfe... 137 2e-34
RIMO_ACTP7 RecName: Full=Ribosomal protein S12 methylthiotransfe... 136 3e-34
MIAB_SULMW RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 136 3e-34
RIMO_ACTPJ RecName: Full=Ribosomal protein S12 methylthiotransfe... 136 4e-34
CK5P1_ORYSJ RecName: Full=CDK5RAP1-like protein; 136 4e-34
RIMO_SORC5 RecName: Full=Ribosomal protein S12 methylthiotransfe... 135 5e-34
RIMO_FRATO RecName: Full=Ribosomal protein S12 methylthiotransfe... 135 5e-34
RIMO_FRATF RecName: Full=Ribosomal protein S12 methylthiotransfe... 135 5e-34
CK5P1_DROME RecName: Full=CDK5RAP1-like protein; 135 5e-34
CK5P1_ARATH RecName: Full=CDK5RAP1-like protein; 135 7e-34
RIMO_ACTP2 RecName: Full=Ribosomal protein S12 methylthiotransfe... 135 9e-34
Y269_TREPA RecName: Full=Putative methylthiotransferase TP_0269;... 134 1e-33
RIMO_SOLUE RecName: Full=Ribosomal protein S12 methylthiotransfe... 134 1e-33
RIMO_HAES2 RecName: Full=Ribosomal protein S12 methylthiotransfe... 134 1e-33
RIMO_HAES1 RecName: Full=Ribosomal protein S12 methylthiotransfe... 134 1e-33
RIMO_THET2 RecName: Full=Ribosomal protein S12 methylthiotransfe... 134 1e-33
RIMO_SHEPC RecName: Full=Ribosomal protein S12 methylthiotransfe... 133 3e-33
RIMO_PSYA2 RecName: Full=Ribosomal protein S12 methylthiotransfe... 133 3e-33
RIMO_MARAV RecName: Full=Ribosomal protein S12 methylthiotransfe... 133 3e-33
RIMO_CHRVO RecName: Full=Ribosomal protein S12 methylthiotransfe... 133 3e-33
RIMO_CAMFF RecName: Full=Ribosomal protein S12 methylthiotransfe... 133 3e-33
CK5P1_HUMAN RecName: Full=CDK5 regulatory subunit-associated pro... 133 3e-33
RIMO_SHESW RecName: Full=Ribosomal protein S12 methylthiotransfe... 133 3e-33
RIMO_NITSB RecName: Full=Ribosomal protein S12 methylthiotransfe... 133 3e-33
RIMO_BACV8 RecName: Full=Ribosomal protein S12 methylthiotransfe... 133 3e-33
RIMO_PSYWF RecName: Full=Ribosomal protein S12 methylthiotransfe... 132 4e-33
RIMO_PSEPF RecName: Full=Ribosomal protein S12 methylthiotransfe... 132 4e-33
RIMO_KINRD RecName: Full=Ribosomal protein S12 methylthiotransfe... 132 4e-33
RIMO_BURPP RecName: Full=Ribosomal protein S12 methylthiotransfe... 132 4e-33
RIMO_AZOSE RecName: Full=Ribosomal protein S12 methylthiotransfe... 132 4e-33
RIMO_PSEU5 RecName: Full=Ribosomal protein S12 methylthiotransfe... 132 6e-33
RIMO_PSEAB RecName: Full=Ribosomal protein S12 methylthiotransfe... 132 6e-33
RIMO_ACTSZ RecName: Full=Ribosomal protein S12 methylthiotransfe... 132 6e-33
RIMO_RHILO RecName: Full=Ribosomal protein S12 methylthiotransfe... 132 7e-33
RIMO_PSEF5 RecName: Full=Ribosomal protein S12 methylthiotransfe... 132 7e-33
RIMO_CHLL2 RecName: Full=Ribosomal protein S12 methylthiotransfe... 132 7e-33
RIMO_SYNFM RecName: Full=Ribosomal protein S12 methylthiotransfe... 131 1e-32
RIMO_PSYCK RecName: Full=Ribosomal protein S12 methylthiotransfe... 131 1e-32
RIMO_PSEU2 RecName: Full=Ribosomal protein S12 methylthiotransfe... 131 1e-32
RIMO_PSEMY RecName: Full=Ribosomal protein S12 methylthiotransfe... 131 1e-32
RIMO_MANSM RecName: Full=Ribosomal protein S12 methylthiotransfe... 131 1e-32
RIMO_CHLPN RecName: Full=Ribosomal protein S12 methylthiotransfe... 131 1e-32
RIMO_RHOPS RecName: Full=Ribosomal protein S12 methylthiotransfe... 131 1e-32
RIMO_RALME RecName: Full=Ribosomal protein S12 methylthiotransfe... 131 1e-32
RIMO_BURXL RecName: Full=Ribosomal protein S12 methylthiotransfe... 131 1e-32
RIMO_STRGG RecName: Full=Ribosomal protein S12 methylthiotransfe... 130 2e-32
RIMO_PSE14 RecName: Full=Ribosomal protein S12 methylthiotransfe... 130 2e-32
RIMO_SHEB8 RecName: Full=Ribosomal protein S12 methylthiotransfe... 130 2e-32
RIMO_PSEAE RecName: Full=Ribosomal protein S12 methylthiotransfe... 130 2e-32
RIMO_MAGSA RecName: Full=Ribosomal protein S12 methylthiotransfe... 130 2e-32
RIMO_LEGPL RecName: Full=Ribosomal protein S12 methylthiotransfe... 130 2e-32
RIMO_LEGPC RecName: Full=Ribosomal protein S12 methylthiotransfe... 130 2e-32
RIMO_LEGPA RecName: Full=Ribosomal protein S12 methylthiotransfe... 130 2e-32
RIMO_BURTA RecName: Full=Ribosomal protein S12 methylthiotransfe... 130 2e-32
RIMO_SHESR RecName: Full=Ribosomal protein S12 methylthiotransfe... 130 3e-32
RIMO_SHESM RecName: Full=Ribosomal protein S12 methylthiotransfe... 130 3e-32
RIMO_SHESA RecName: Full=Ribosomal protein S12 methylthiotransfe... 130 3e-32
RIMO_SHEB9 RecName: Full=Ribosomal protein S12 methylthiotransfe... 130 3e-32
RIMO_PORG3 RecName: Full=Ribosomal protein S12 methylthiotransfe... 130 3e-32
RIMO_HAEDU RecName: Full=Ribosomal protein S12 methylthiotransfe... 130 3e-32
RIMO_BURCH RecName: Full=Ribosomal protein S12 methylthiotransfe... 130 3e-32
RIMO_BURCA RecName: Full=Ribosomal protein S12 methylthiotransfe... 130 3e-32
RIMO_DEIGD RecName: Full=Ribosomal protein S12 methylthiotransfe... 129 4e-32
RIMO_BURCJ RecName: Full=Ribosomal protein S12 methylthiotransfe... 129 4e-32
RIMO_AZOSB RecName: Full=Ribosomal protein S12 methylthiotransfe... 129 4e-32
RIMO_ANADE RecName: Full=Ribosomal protein S12 methylthiotransfe... 129 4e-32
MIAB_SORC5 RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 129 4e-32
RIMO_UNCTG RecName: Full=Ribosomal protein S12 methylthiotransfe... 129 5e-32
RIMO_SPHAL RecName: Full=Ribosomal protein S12 methylthiotransfe... 129 5e-32
RIMO_SHEON RecName: Full=Ribosomal protein S12 methylthiotransfe... 129 5e-32
RIMO_SHEB5 RecName: Full=Ribosomal protein S12 methylthiotransfe... 129 5e-32
RIMO_RHOPA RecName: Full=Ribosomal protein S12 methylthiotransfe... 129 5e-32
RIMO_RHOP2 RecName: Full=Ribosomal protein S12 methylthiotransfe... 129 5e-32
RIMO_RHOFD RecName: Full=Ribosomal protein S12 methylthiotransfe... 129 5e-32
RIMO_RALPJ RecName: Full=Ribosomal protein S12 methylthiotransfe... 129 5e-32
RIMO_PSEA7 RecName: Full=Ribosomal protein S12 methylthiotransfe... 129 5e-32
RIMO_MYXXD RecName: Full=Ribosomal protein S12 methylthiotransfe... 129 5e-32
RIMO_LEGPH RecName: Full=Ribosomal protein S12 methylthiotransfe... 129 5e-32
RIMO_BURS3 RecName: Full=Ribosomal protein S12 methylthiotransfe... 129 5e-32
RIMO_BURCC RecName: Full=Ribosomal protein S12 methylthiotransfe... 129 5e-32
RIMO_BORPE RecName: Full=Ribosomal protein S12 methylthiotransfe... 129 5e-32
RIMO_BORPA RecName: Full=Ribosomal protein S12 methylthiotransfe... 129 5e-32
RIMO_ACISJ RecName: Full=Ribosomal protein S12 methylthiotransfe... 129 5e-32
RIMO_RHOPT RecName: Full=Ribosomal protein S12 methylthiotransfe... 129 6e-32
RIMO_PORGI RecName: Full=Ribosomal protein S12 methylthiotransfe... 129 6e-32
RIMO_RALSO RecName: Full=Ribosomal protein S12 methylthiotransfe... 128 8e-32
RIMO_ACIC1 RecName: Full=Ribosomal protein S12 methylthiotransfe... 128 8e-32
RIMO_RHOCS RecName: Full=Ribosomal protein S12 methylthiotransfe... 128 1e-31
RIMO_BURVG RecName: Full=Ribosomal protein S12 methylthiotransfe... 128 1e-31
RIMO_BURM1 RecName: Full=Ribosomal protein S12 methylthiotransfe... 128 1e-31
RIMO_ZYMMO RecName: Full=Ribosomal protein S12 methylthiotransfe... 127 1e-31
RIMO_PSESM RecName: Full=Ribosomal protein S12 methylthiotransfe... 127 1e-31
RIMO_SACD2 RecName: Full=Ribosomal protein S12 methylthiotransfe... 127 2e-31
RIMO_CELJU RecName: Full=Ribosomal protein S12 methylthiotransfe... 127 2e-31
RIMO_BURMS RecName: Full=Ribosomal protein S12 methylthiotransfe... 127 2e-31
RIMO_BURMA RecName: Full=Ribosomal protein S12 methylthiotransfe... 127 2e-31
RIMO_BURM9 RecName: Full=Ribosomal protein S12 methylthiotransfe... 127 2e-31
RIMO_BURM7 RecName: Full=Ribosomal protein S12 methylthiotransfe... 127 2e-31
RIMO_BURCM RecName: Full=Ribosomal protein S12 methylthiotransfe... 127 2e-31
MIAB_BUCCC RecName: Full=(Dimethylallyl)adenosine tRNA methylthi... 127 2e-31
RIMO_SHEAM RecName: Full=Ribosomal protein S12 methylthiotransfe... 127 2e-31
RIMO_DESOH RecName: Full=Ribosomal protein S12 methylthiotransfe... 127 2e-31
RIMO_BURPS RecName: Full=Ribosomal protein S12 methylthiotransfe... 127 2e-31
RIMO_BURP1 RecName: Full=Ribosomal protein S12 methylthiotransfe... 127 2e-31
RIMO_BURP0 RecName: Full=Ribosomal protein S12 methylthiotransfe... 127 2e-31
RIMO_BORPD RecName: Full=Ribosomal protein S12 methylthiotransfe... 127 2e-31
RIMO_ANADF RecName: Full=Ribosomal protein S12 methylthiotransfe... 127 2e-31
RIMO_POLSQ RecName: Full=Ribosomal protein S12 methylthiotransfe... 126 3e-31
RIMO_AZOC5 RecName: Full=Ribosomal protein S12 methylthiotransfe... 126 3e-31
RIMO_POLNS RecName: Full=Ribosomal protein S12 methylthiotransfe... 126 4e-31
RIMO_CAMC1 RecName: Full=Ribosomal protein S12 methylthiotransfe... 126 4e-31
RIMO_METS4 RecName: Full=Ribosomal protein S12 methylthiotransfe... 125 5e-31
RIMO_METPB RecName: Full=Ribosomal protein S12 methylthiotransfe... 125 5e-31
RIMO_METEP RecName: Full=Ribosomal protein S12 methylthiotransfe... 125 5e-31
RIMO_DECAR RecName: Full=Ribosomal protein S12 methylthiotransfe... 125 5e-31
RIMO_ACIF2 RecName: Full=Ribosomal protein S12 methylthiotransfe... 125 5e-31
RIMO_METC4 RecName: Full=Ribosomal protein S12 methylthiotransfe... 125 7e-31
RIMO_BURA4 RecName: Full=Ribosomal protein S12 methylthiotransfe... 125 7e-31
RIMO_BURP8 RecName: Full=Ribosomal protein S12 methylthiotransfe... 125 9e-31
RIMO_THEFY RecName: Full=Ribosomal protein S12 methylthiotransfe... 124 1e-30
RIMO_SPHWW RecName: Full=Ribosomal protein S12 methylthiotransfe... 124 1e-30
RIMO_RALEJ RecName: Full=Ribosomal protein S12 methylthiotransfe... 124 1e-30
RIMO_HYDS0 RecName: Full=Ribosomal protein S12 methylthiotransfe... 124 1e-30
RIMO_CHLTE RecName: Full=Ribosomal protein S12 methylthiotransfe... 124 1e-30
RIMO_BORBR RecName: Full=Ribosomal protein S12 methylthiotransfe... 124 1e-30
RIMO_XANCP RecName: Full=Ribosomal protein S12 methylthiotransfe... 124 2e-30
RIMO_XANC8 RecName: Full=Ribosomal protein S12 methylthiotransfe... 124 2e-30
RIMO_XANAC RecName: Full=Ribosomal protein S12 methylthiotransfe... 124 2e-30
RIMO_RHOP5 RecName: Full=Ribosomal protein S12 methylthiotransfe... 124 2e-30
RIMO_BURP6 RecName: Full=Ribosomal protein S12 methylthiotransfe... 124 2e-30
RIMO_BRASB RecName: Full=Ribosomal protein S12 methylthiotransfe... 124 2e-30
RIMO_XANCB RecName: Full=Ribosomal protein S12 methylthiotransfe... 124 2e-30
RIMO_JANMA RecName: Full=Ribosomal protein S12 methylthiotransfe... 123 3e-30
RIMO_METRJ RecName: Full=Ribosomal protein S12 methylthiotransfe... 123 3e-30
RIMO_CUPTR RecName: Full=Ribosomal protein S12 methylthiotransfe... 123 3e-30
RIMO_ROSS1 RecName: Full=Ribosomal protein S12 methylthiotransfe... 122 4e-30
RIMO_RHOPB RecName: Full=Ribosomal protein S12 methylthiotransfe... 122 4e-30
RIMO_METFK RecName: Full=Ribosomal protein S12 methylthiotransfe... 122 4e-30
RIMO_COLP3 RecName: Full=Ribosomal protein S12 methylthiotransfe... 122 4e-30
RIMO_XANC5 RecName: Full=Ribosomal protein S12 methylthiotransfe... 122 6e-30
RIMO_RHORT RecName: Full=Ribosomal protein S12 methylthiotransfe... 122 6e-30
RIMO_STRMK RecName: Full=Ribosomal protein S12 methylthiotransfe... 122 8e-30
RIMO_STRM5 RecName: Full=Ribosomal protein S12 methylthiotransfe... 122 8e-30
RIMO_RALEH RecName: Full=Ribosomal protein S12 methylthiotransfe... 122 8e-30
RIMO_BRASO RecName: Full=Ribosomal protein S12 methylthiotransfe... 122 8e-30
RIMO_POLNA RecName: Full=Ribosomal protein S12 methylthiotransfe... 121 1e-29
RIMO_CHLP8 RecName: Full=Ribosomal protein S12 methylthiotransfe... 121 1e-29
RIMO_NITEU RecName: Full=Ribosomal protein S12 methylthiotransfe... 120 2e-29
RIMO_HELPG RecName: Full=Ribosomal protein S12 methylthiotransfe... 120 2e-29
RIMO_ACIF5 RecName: Full=Ribosomal protein S12 methylthiotransfe... 120 2e-29
RIMO_HERAR RecName: Full=Ribosomal protein S12 methylthiotransfe... 120 2e-29
RIMO_ERYLH RecName: Full=Ribosomal protein S12 methylthiotransfe... 120 2e-29
RIMO_ALTMD RecName: Full=Ribosomal protein S12 methylthiotransfe... 120 2e-29
RIMO_HELPH RecName: Full=Ribosomal protein S12 methylthiotransfe... 120 3e-29
RIMO_CHLPB RecName: Full=Ribosomal protein S12 methylthiotransfe... 120 3e-29
RIMO_BRAJA RecName: Full=Ribosomal protein S12 methylthiotransfe... 120 3e-29
RIMO_BRUO2 RecName: Full=Ribosomal protein S12 methylthiotransfe... 119 4e-29
RIMO_XANOR RecName: Full=Ribosomal protein S12 methylthiotransfe... 119 5e-29
RIMO_XANOP RecName: Full=Ribosomal protein S12 methylthiotransfe... 119 5e-29
RIMO_XANOM RecName: Full=Ribosomal protein S12 methylthiotransfe... 119 5e-29
RIMO_HELP2 RecName: Full=Ribosomal protein S12 methylthiotransfe... 119 5e-29
RIMO_POLSJ RecName: Full=Ribosomal protein S12 methylthiotransfe... 119 6e-29
RIMO_OCHA4 RecName: Full=Ribosomal protein S12 methylthiotransfe... 119 6e-29
RIMO_BRUSU RecName: Full=Ribosomal protein S12 methylthiotransfe... 119 6e-29
RIMO_BRUSI RecName: Full=Ribosomal protein S12 methylthiotransfe... 119 6e-29
RIMO_BRUME RecName: Full=Ribosomal protein S12 methylthiotransfe... 119 6e-29
RIMO_BRUC2 RecName: Full=Ribosomal protein S12 methylthiotransfe... 119 6e-29
RIMO_BRUAB RecName: Full=Ribosomal protein S12 methylthiotransfe... 119 6e-29
RIMO_BRUA2 RecName: Full=Ribosomal protein S12 methylthiotransfe... 119 6e-29
RIMO_BRUA1 RecName: Full=Ribosomal protein S12 methylthiotransfe... 119 6e-29
RIMO_TREDE RecName: Full=Ribosomal protein S12 methylthiotransfe... 118 8e-29
RIMO_OLICO RecName: Full=Ribosomal protein S12 methylthiotransfe... 118 8e-29
RIMO_DELAS RecName: Full=Ribosomal protein S12 methylthiotransfe... 117 1e-28
RIMO_VEREI RecName: Full=Ribosomal protein S12 methylthiotransfe... 117 2e-28
RIMO_HELPJ RecName: Full=Ribosomal protein S12 methylthiotransfe... 117 2e-28
RIMO_HELAH RecName: Full=Ribosomal protein S12 methylthiotransfe... 117 2e-28
RIMO_DEIRA RecName: Full=Ribosomal protein S12 methylthiotransfe... 117 2e-28
RIMO_AKKM8 RecName: Full=Ribosomal protein S12 methylthiotransfe... 117 2e-28
RIMO_BART1 RecName: Full=Ribosomal protein S12 methylthiotransfe... 117 2e-28
RIMO_HELPS RecName: Full=Ribosomal protein S12 methylthiotransfe... 116 3e-28
RIMO_CHLCH RecName: Full=Ribosomal protein S12 methylthiotransfe... 116 3e-28
RIMO_BARHE RecName: Full=Ribosomal protein S12 methylthiotransfe... 116 3e-28
RIMO_RHOS1 RecName: Full=Ribosomal protein S12 methylthiotransfe... 115 5e-28
RIMO_PELPB RecName: Full=Ribosomal protein S12 methylthiotransfe... 115 5e-28
RIMO_CHLPD RecName: Full=Ribosomal protein S12 methylthiotransfe... 115 7e-28
RIMO_NITMU RecName: Full=Ribosomal protein S12 methylthiotransfe... 115 9e-28
RIMO_RHOS4 RecName: Full=Ribosomal protein S12 methylthiotransfe... 114 1e-27
RIMO_HELPY RecName: Full=Ribosomal protein S12 methylthiotransfe... 114 1e-27
RIMO_LEPCP RecName: Full=Ribosomal protein S12 methylthiotransfe... 114 2e-27
RIMO_METCA RecName: Full=Ribosomal protein S12 methylthiotransfe... 114 2e-27
RIMO_CHLT3 RecName: Full=Ribosomal protein S12 methylthiotransfe... 113 4e-27
RIMO_RHOS5 RecName: Full=Ribosomal protein S12 methylthiotransfe... 112 6e-27
RIMO_PELLD RecName: Full=Ribosomal protein S12 methylthiotransfe... 111 1e-26
RIMO_METPP RecName: Full=Ribosomal protein S12 methylthiotransfe... 111 1e-26
RIMO_NOVAD RecName: Full=Ribosomal protein S12 methylthiotransfe... 111 1e-26
RIMO_NITEC RecName: Full=Ribosomal protein S12 methylthiotransfe... 111 1e-26
RIMO_XANP2 RecName: Full=Ribosomal protein S12 methylthiotransfe... 110 2e-26
RIMO_SALTO RecName: Full=Ribosomal protein S12 methylthiotransfe... 110 2e-26
RIMO_FRASC RecName: Full=Ribosomal protein S12 methylthiotransfe... 110 2e-26
RIMO_ARCB4 RecName: Full=Ribosomal protein S12 methylthiotransfe... 110 2e-26
RIMO_PROVI RecName: Full=Ribosomal protein S12 methylthiotransfe... 110 3e-26
RIMO_PARDP RecName: Full=Ribosomal protein S12 methylthiotransfe... 109 4e-26
RIMO_ALHEH RecName: Full=Ribosomal protein S12 methylthiotransfe... 109 4e-26
RIMO_LAWIP RecName: Full=Ribosomal protein S12 methylthiotransfe... 108 7e-26
RIMO_SULDN RecName: Full=Ribosomal protein S12 methylthiotransfe... 108 9e-26
RIMO_BEII9 RecName: Full=Ribosomal protein S12 methylthiotransfe... 108 9e-26
RIMO_SALAI RecName: Full=Ribosomal protein S12 methylthiotransfe... 107 1e-25
RIMO_ACIAC RecName: Full=Ribosomal protein S12 methylthiotransfe... 107 2e-25
RIMO_PROA2 RecName: Full=Ribosomal protein S12 methylthiotransfe... 107 3e-25
RIMO_ROSCS RecName: Full=Ribosomal protein S12 methylthiotransfe... 106 3e-25
RIMO_JANSC RecName: Full=Ribosomal protein S12 methylthiotransfe... 106 3e-25
RIMO_SILPO RecName: Full=Ribosomal protein S12 methylthiotransfe... 104 1e-24
RIMO_DINSH RecName: Full=Ribosomal protein S12 methylthiotransfe... 103 3e-24
RIMO_ROSDO RecName: Full=Ribosomal protein S12 methylthiotransfe... 102 6e-24
RIMO_SILST RecName: Full=Ribosomal protein S12 methylthiotransfe... 100 2e-23
RIMO_DESDG RecName: Full=Ribosomal protein S12 methylthiotransfe... 100 2e-23
RIMO_HYPNA RecName: Full=Ribosomal protein S12 methylthiotransfe... 99 5e-23
CDKAL_CAEEL RecName: Full=CDKAL1-like protein; 99 7e-23
RIMO_DESVV RecName: Full=Ribosomal protein S12 methylthiotransfe... 97 3e-22
RIMO_DESVH RecName: Full=Ribosomal protein S12 methylthiotransfe... 97 3e-22
RIMO_FRAAA RecName: Full=Ribosomal protein S12 methylthiotransfe... 93 4e-21
Y1620_METJA RecName: Full=Uncharacterized protein MJ1620; 46 7e-07
KAMA_BACSU RecName: Full=L-lysine 2,3-aminomutase; Short... 40 5e-05
LIPA_ALKMQ RecName: Full=Lipoyl synthase; EC=2.8.1.8;Alt... 37 2e-04
BCHE_RHOS4 RecName: Full=Anaerobic magnesium-protoporphyrin IX m... 37 2e-04
BCHE_RHOGE RecName: Full=Anaerobic magnesium-protoporphyrin IX m... 36 7e-04
>YQEV_BACSU RecName: Full=Putative methylthiotransferase yqeV;
EC=2.-.-.-;
Length = 451
Score = 363 bits (933), Expect = e-102
Identities = 185/435 (42%), Positives = 285/435 (65%), Gaps = 6/435 (1%)
Query: 3 VAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIGR 62
VAF TLGC+VN YE+EA+ + F GYE DF + ADVYV+NTC+VTN GDKKSRQ+I R
Sbjct: 4 VAFHTLGCKVNHYETEAIWQLFKEAGYERRDFEQTADVYVINTCTVTNTGDKKSRQVIRR 63
Query: 63 ARRQNPEAIIAVVGCYAQIAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVKVG 122
A RQNP+ +I V GCYAQ +P E+ I GVD+V+G++++ +++ Y+++ R+E + V
Sbjct: 64 AIRQNPDGVICVTGCYAQTSPAEIMAIPGVDIVVGTQDREKMLGYIDQYREERQPINGVS 123
Query: 123 AVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDEIRS 182
++K +V+E+L + + ++TRA LKIQ+GCN FC +C+IP+ RG + S+DP++V+ + +
Sbjct: 124 NIMKARVYEELDVPAFTDRTRASLKIQEGCNNFCTFCIIPWARGLLRSRDPEEVIKQAQQ 183
Query: 183 LAEHGFKEIILSGIHTASYGVDLDEKVTLVDLLEEIE-KIDGIERVRIGSIDPTFFTEDV 241
L + G+KEI+L+GIHT YG D+ + LL E++ +++G++R+RI SI+ + T++V
Sbjct: 184 LVDAGYKEIVLTGIHTGGYGEDMKD-YNFAKLLSELDTRVEGVKRIRISSIEASQITDEV 242
Query: 242 VRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITTDVIV 301
+ + K+ H H+ +QSG + LKRM R+YT + + D +N L+ + +++T+DVIV
Sbjct: 243 IEVLDRSDKIVNHLHIPIQSGSNTVLKRMRRKYTMEFFADRLNKLKKALPGLAVTSDVIV 302
Query: 302 GFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSKKLIEL 361
GFPG +K S+ H+F YS R GT A ME+QVD N K+ER +LI L
Sbjct: 303 GFPGETEEEFMETYNFIKEHKFSELHVFPYSKRTGTPAARMEDQVDENVKNERVHRLIAL 362
Query: 362 NRINEKAFAEKYIGEVIDVLFEE---EVELGSGVYTGYTRNYIKVNAKADCNVSGKILNV 418
+ K +A +Y EV++++ EE E E ++ GYT NY+KV K ++ GKI+ V
Sbjct: 363 SDQLAKEYASQYENEVLEIIPEEAFKETE-EENMFVGYTDNYMKVVFKGTEDMIGKIVKV 421
Query: 419 KINSFEGEVAEGEIV 433
KI EG+ V
Sbjct: 422 KILKAGYPYNEGQFV 436
>Y474_AQUAE RecName: Full=Putative methylthiotransferase aq_474;
EC=2.-.-.-;
Length = 410
Score = 318 bits (816), Expect = 4e-89
Identities = 170/420 (40%), Positives = 263/420 (62%), Gaps = 10/420 (2%)
Query: 1 MKVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
MKVAF TLGCR+N ++++ + KF+++GYEVV F +MADVYV+NTC+VT GD+ SRQ I
Sbjct: 1 MKVAFETLGCRMNQFDTDLLKNKFIQKGYEVVSFEDMADVYVINTCTVTVGGDRSSRQAI 60
Query: 61 GRARRQNPEAIIAVVGCYAQIAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVK 120
+A+R+NP+AI+ GCYAQ+ P E++ ++ VD+V+G+ +K E++ + + + +++V
Sbjct: 61 YQAKRRNPKAIVVATGCYAQVNPQELAKLKEVDLVVGNTHKSELLKILEEYLERREKKVV 120
Query: 121 VGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDEI 180
VG + + K + Y R FLK+Q+GCN+FC +C+IPY RG V S D +K++ ++
Sbjct: 121 VGEIFREKEVRNFDTVLYFEGVRPFLKVQEGCNKFCTFCVIPYARGKVRSVDLEKIVHQV 180
Query: 181 RSLAEHGFKEIILSGIHTASYGVDLDEKVTLVDLLEEIEKIDGIERVRIGSIDPTFFTED 240
+ LA+ GFKE++L+G + YG D+ L LL E+ KI+GIE +R+ S+ ++
Sbjct: 181 KLLAQKGFKEVVLTGTQLSQYG--WDKGYNLYTLLTELIKIEGIELIRLSSMHIKEMDKE 238
Query: 241 VVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITTDVI 300
+++ I++ +K+ PHFHLSLQSG + L+ M+R YT +EYE++VN + + SI TDVI
Sbjct: 239 LLKLIVSEEKIAPHFHLSLQSGSNRILELMDRGYTREEYEEVVNFIVENRPISSIGTDVI 298
Query: 301 VGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSKKLIE 360
VGFP LKRI +S HIF YS R T+A ++ ++ K ER + L E
Sbjct: 299 VGFPTESEEDFQETYEFLKRIPISYMHIFPYSDRPFTKASKLKPKLPERIKKERVRILKE 358
Query: 361 LNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSGKILNVKI 420
L++ + F EK G+ + L EE L T NYI + + V GK++ V I
Sbjct: 359 LDQKKRQEFYEKNKGKELRALVIEENRL-------LTENYIDIKREGYKEV-GKLVRVLI 410
>Y16R_MYCIO RecName: Full=Putative methylthiotransferase in 16S RNA
5'region; EC=2.-.-.-;
Length = 438
Score = 273 bits (698), Expect = 2e-75
Identities = 157/433 (36%), Positives = 245/433 (56%), Gaps = 9/433 (2%)
Query: 4 AFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIGRA 63
A TLGC+VN +ES ++ + G V F+ ADVY++NTC+VTN D KSR I RA
Sbjct: 11 AIHTLGCKVNLFESNSIKNDLIMNGLVEVPFDSKADVYIINTCTVTNKADAKSRLYIKRA 70
Query: 64 RRQNPEAIIAVVGCYAQIAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVKVGA 123
QN +AII V GC +Q+ + ++ + + +G++ K + +N+ + ++ +V
Sbjct: 71 HVQNKDAIIIVAGCMSQVNKDLMDKLK-ISIQIGNKYKNSVFDLINEYLKKRERIYRVEN 129
Query: 124 VLKNKVFEDLKIEE-YNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDEIRS 182
+L K FE + + TRAF+KIQDGCN C+YC+IP++RG S+ + +L++I++
Sbjct: 130 ILAEKKFEQTTQDFIFLENTRAFIKIQDGCNFMCSYCIIPFSRGRQRSQKMESILEKIKT 189
Query: 183 LAEHGFKEIILSGIHTASYGVDLDEKV-TLVDLLEEIEKIDGIERVRIGSIDPTFFTEDV 241
L FKEI+L+G++TA Y LDE T DLL I K+DG RVRI S++P +++
Sbjct: 190 LVSKXFKEIVLTGVNTAGY---LDENNNTFFDLLNNINKLDGDFRVRISSLEPFQINDEI 246
Query: 242 VRRILALKK-LCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITTDVI 300
+ + + KK C H+H+ LQSG D LK+MNR+YT E+ ++ + +K + TTD I
Sbjct: 247 IDLVTSNKKRFCQHWHICLQSGSDDVLKKMNRKYTTDEFYKLMQKILNKSPLTNFTTDYI 306
Query: 301 VGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSKKLIE 360
VGFP L +I L HIF YS R TR+ + ++ D KKD R K++
Sbjct: 307 VGFPTETDEDQKKSIDFLNKIKLYDMHIFPYSKRNNTRSSHYKDINDSTKKD-RVKEITX 365
Query: 361 LNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSGKILNVKI 420
IG+ +VLFE++ E +++GY+ Y +V ++ N+ K+L V+I
Sbjct: 366 XXXXXXXXXXXXXIGKTCEVLFEKKKE-DEKMWSGYSNEYCRVMVESGDNLENKMLKVRI 424
Query: 421 NSFEGEVAEGEIV 433
+ GEI+
Sbjct: 425 KKIFFDSLVGEII 437
>MIAB_CLOTE RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 453
Score = 261 bits (666), Expect = 1e-71
Identities = 153/433 (35%), Positives = 235/433 (54%), Gaps = 8/433 (1%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIGRAR-- 64
T GC++N +SE ++ GY+ + AD+ + NTC V + K +G +
Sbjct: 23 TWGCQMNEEDSEKLSGMLKNIGYKNAEDKNQADIIIFNTCCVRENAELKVYGNLGALKGL 82
Query: 65 -RQNPEAIIAVVGCYAQ---IAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVK 120
+NP IIAV GC Q +A + VD++ G+ N + Y+N+A+ EGK ++
Sbjct: 83 KSKNPNLIIAVCGCMMQQEGMAEAIIKKYPFVDIIFGTHNSYKFPEYLNRAKQEGKSIIE 142
Query: 121 VGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDEI 180
V + ++ E + ++ + T+AF+ I GCN FC YC++PY RG S++ + EI
Sbjct: 143 VWDK-EEEIVEGIPVDR-KSSTKAFVTIMYGCNNFCTYCIVPYVRGRERSREVSDIEKEI 200
Query: 181 RSLAEHGFKEIILSGIHTASYGVDLDEKVTLVDLLEEIEKIDGIERVRIGSIDPTFFTED 240
+ L + G+KEI L G + SYG DL+ KV+ +LL + +I+GIERVR + P TED
Sbjct: 201 KELVKSGYKEITLLGQNVNSYGKDLEPKVSFAELLRHVNEIEGIERVRFMTSHPKDLTED 260
Query: 241 VVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITTDVI 300
V+ I KLC H HL +QSG LK+MNR Y ++Y ++VN ++++I +V+ITTD+I
Sbjct: 261 VIYAIKECDKLCNHIHLPVQSGSSRILKKMNRYYNREDYLNLVNKIKEEIPNVAITTDII 320
Query: 301 VGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSKKLIE 360
VGFPG +K + F YS RKGT A ++E QV + K +R KKL+E
Sbjct: 321 VGFPGETEEDFNETLELVKEVEYDSAFTFLYSKRKGTPAYDIEEQVPEDVKHDRFKKLVE 380
Query: 361 LNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSGKILNVKI 420
+ + + +KY ++ VL E E + +G T VN + + GKI++VKI
Sbjct: 381 VVNKSCEKNNKKYQDRIVKVLVEGESKNDKNKLSGRTDTAKLVNFIGNKDNIGKIVDVKI 440
Query: 421 NSFEGEVAEGEIV 433
EGE V
Sbjct: 441 TKTLSFSLEGEEV 453
>MIAB_CLOBM RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 450
Score = 259 bits (663), Expect = 2e-71
Identities = 156/427 (36%), Positives = 232/427 (54%), Gaps = 13/427 (3%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKK---SRQIIGRA 63
T GC++N +SE ++ +EGY + E ADV + NTC V + K + I+
Sbjct: 20 TWGCQMNEEDSEKLSGMLKKEGYIRTEERENADVIIFNTCCVRENAELKVYGNLGILKGL 79
Query: 64 RRQNPEAIIAVVGCYAQ---IAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVK 120
+ +NP IIAV GC Q +A T VD+++G+ N Y+N+ + + +K
Sbjct: 80 KSKNPNLIIAVTGCMMQQKGMAETIKKKFPFVDIIIGTHNLHNFPNYLNEVKKKDTSILK 139
Query: 121 VGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDEI 180
+ ++ + E++ I+ N +AF+ I GCN FC YC++PY RG S+ P+ + DEI
Sbjct: 140 IQEK-EDSIIENMPIDR-KNSMKAFVTIMYGCNNFCTYCIVPYVRGRERSRTPENIEDEI 197
Query: 181 RSLAEHGFKEIILSGIHTASYGVDLDEKVTLVDLLEEIEKIDGIERVRIGSIDPTFFTED 240
+ L G+KEI L G + SYG DL+ KVT DLLE + IDG+ERVR + P T+D
Sbjct: 198 KKLISEGYKEITLLGQNVNSYGKDLEPKVTFADLLERVNTIDGLERVRFMTSHPKDLTDD 257
Query: 241 VVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITTDVI 300
V+ I KLC HL +QSG LK+MNR Y ++Y D+V+ ++ I +V+++TD+I
Sbjct: 258 VIEAIAKCDKLCEQIHLPVQSGSSEILKKMNRHYDREKYLDVVSKIKKLIPNVALSTDII 317
Query: 301 VGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSKKLIE 360
VGFPG +K + F YS RKGT A E+QV + K +R +L+E
Sbjct: 318 VGFPGETEKDFEETLSLVKEVEYDSAFTFLYSIRKGTPAAKFEDQVPEDVKHKRFNRLVE 377
Query: 361 -LNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSGKILNVK 419
+N I+ K + Y G++ +VL E + G TR VN D + GK++NVK
Sbjct: 378 VVNEISAKK-NKAYEGKIEEVLVEGTSKNDENKLMGRTRTGKLVNFIGDKDSIGKLVNVK 436
Query: 420 I---NSF 423
I NSF
Sbjct: 437 IIKANSF 443
>MIAB_CLOBK RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 450
Score = 258 bits (660), Expect = 5e-71
Identities = 155/427 (36%), Positives = 232/427 (54%), Gaps = 13/427 (3%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKK---SRQIIGRA 63
T GC++N +SE ++ +EGY + E ADV + NTC V + K + I+
Sbjct: 20 TWGCQMNEEDSEKLSGMLKKEGYIRTEERENADVIIFNTCCVRENAELKVYGNLGILKGL 79
Query: 64 RRQNPEAIIAVVGCYAQ---IAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVK 120
+ +NP IIAV GC Q +A T VD+++G+ N Y+N+ + + +K
Sbjct: 80 KSKNPNLIIAVTGCMMQQKGMAETIKKKFPFVDIIIGTHNLHNFPNYLNEVKKKDTSVLK 139
Query: 121 VGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDEI 180
+ ++ + E++ I+ N +AF+ I GCN FC YC++PY RG S+ P+ + DEI
Sbjct: 140 IQEK-EDSIIENMPIDR-KNSMKAFVTIMYGCNNFCTYCIVPYVRGRERSRTPENIEDEI 197
Query: 181 RSLAEHGFKEIILSGIHTASYGVDLDEKVTLVDLLEEIEKIDGIERVRIGSIDPTFFTED 240
+ L G+KEI L G + SYG DL+ KVT +LLE + IDG+ERVR + P T+D
Sbjct: 198 KKLISEGYKEITLLGQNVNSYGKDLEPKVTFAELLERVNTIDGLERVRFMTSHPKDLTDD 257
Query: 241 VVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITTDVI 300
V+ I KLC HL +QSG LK+MNR Y ++Y D+V+ ++ I +V+++TD+I
Sbjct: 258 VIEAIAKCDKLCEQIHLPVQSGSSEILKKMNRHYDREKYLDVVSKIKKLIPNVALSTDII 317
Query: 301 VGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSKKLIE 360
VGFPG +K + F YS RKGT A E+QV + K +R +L+E
Sbjct: 318 VGFPGETEKDFEETLSLVKEVEYDSAFTFLYSIRKGTPAAKFEDQVPEDVKHKRFNRLVE 377
Query: 361 -LNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSGKILNVK 419
+N I+ K + Y G++ +VL E + G TR VN D + GK++NVK
Sbjct: 378 VVNEISAKK-NKAYEGKIEEVLVEGTSKNDENKLMGRTRTGKLVNFIGDKDSIGKLVNVK 436
Query: 420 I---NSF 423
I NSF
Sbjct: 437 IIKANSF 443
>Y830_THEMA RecName: Full=Putative methylthiotransferase TM_0830;
EC=2.-.-.-;
Length = 434
Score = 258 bits (658), Expect = 9e-71
Identities = 155/425 (36%), Positives = 237/425 (55%), Gaps = 13/425 (3%)
Query: 3 VAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIGR 62
V T GC+VN YESE M E+ + GY V+ A Y+VN+C+VT +KK +++I
Sbjct: 4 VRIETFGCKVNQYESEYMAEQLEKAGYVVLPDGNAA-YYIVNSCAVTKEVEKKVKRLIKS 62
Query: 63 ARRQNPEAIIAVVGCYAQIAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVKVG 122
R +N A I + GC+AQ++P E + VD+VLG K IV ++N GKQQV V
Sbjct: 63 IRNRNKNAKIILTGCFAQLSPDEAKNLP-VDMVLGIDEKKHIVDHINSLN--GKQQVVVS 119
Query: 123 AVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVL-DEIR 181
+ V+E +K + ++TR+++K++DGC+ C YC I RG+ P ++ +E
Sbjct: 120 EPGR-PVYEKVK-GSFEDRTRSYIKVEDGCDNTCTYCAIRLARGTRIRSKPLEIFKEEFA 177
Query: 182 SLAEHGFKEIILSGIHTASYGVDLDEKVTLVDLLEEIEKIDGIERVRIGSIDPTFFTEDV 241
+ G+KEI+++G++ YG D+ +L +LL+ IEK+ G RVR+ SI+ +++
Sbjct: 178 EMVMKGYKEIVITGVNLGKYGKDMGS--SLAELLKVIEKVPGDYRVRLSSINVEDVNDEI 235
Query: 242 VRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITTDVIV 301
V+ +LCPH H+S+QSG D LKRM R+Y ++ +V+ LR D SITTD+IV
Sbjct: 236 VKAFKRNPRLCPHLHISVQSGSDDVLKRMGRKYKISDFMRVVDKLRSIDPDFSITTDIIV 295
Query: 302 GFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSKKLIEL 361
GFPG ++++ S+ HIF++SPR GT A ME V +KK ER L E
Sbjct: 296 GFPGETDADFQRTLELVEKVEFSRVHIFRFSPRPGTPASRMEGGVPESKKKERLDVLKEK 355
Query: 362 NRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSGKILNVKIN 421
+ + ++ IG+ VL E V GV +GY Y+K N G+ +V++
Sbjct: 356 AKDVSIRYRKRIIGKERKVLAEWYVM--KGVLSGYDEYYVKHEFVG--NRVGEFHSVRVK 411
Query: 422 SFEGE 426
S E
Sbjct: 412 SLSEE 416
>MIAB_THEP3 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 471
Score = 256 bits (654), Expect = 3e-70
Identities = 165/437 (37%), Positives = 254/437 (58%), Gaps = 16/437 (3%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIGRA--- 63
T GC++N ++SE + + GY+ + E ADV + NTC+V + + ++GR
Sbjct: 37 TYGCQMNVHDSEKLAGMLEKMGYKYTENLEQADVLLFNTCAVREHAEIR---VLGRVSQM 93
Query: 64 ---RRQNPEAIIAVVGCYAQ---IAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGKQ 117
+ +NP II V GC Q + +D+V G+ N + + +A +
Sbjct: 94 KELKARNPNLIIGVSGCMMQEKNVVEAIKEKYSYIDIVFGTHNIYKFPQLLWEALNSQDI 153
Query: 118 QVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVL 177
+ + KN V E+L ++ +N +A++ I GCN FC YC++PYTRG S+ P+ ++
Sbjct: 154 VIDIIEDTKN-VIEELPVKRDSN-LKAWVNIIYGCNNFCTYCIVPYTRGREKSRKPEDII 211
Query: 178 DEIRSLAEHGFKEIILSGIHTASYGVDLDEKVTLVDLLEEIEKIDGIERVRIGSIDPTFF 237
E++ LA+ G+KEI L G + SYG DLDE +T LL ++ I+GIER+R + P
Sbjct: 212 AEVKELAQKGYKEITLLGQNVNSYGKDLDEDITFAKLLYKLNDIEGIERIRFMTSHPKDI 271
Query: 238 TEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITT 297
+++++ I L K+C H HL +Q+G + LK+MNR+YT + Y +I++ +R I D++ITT
Sbjct: 272 SDELIYAIRDLDKVCEHLHLPVQAGSNKILKKMNRKYTKEHYLEIIDKVRSNIPDIAITT 331
Query: 298 DVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSKK 357
D+IVGFPG R+ + F YS R GT A NM +QVD K ER ++
Sbjct: 332 DIIVGFPGXXXXXXXXXXXXXXRVRFDAAYTFIYSKRAGTVAANMPDQVDDAVKHERLER 391
Query: 358 LIEL-NRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSGKIL 416
LIEL N+I+ + AE G++++VL E + S T TR V+ D ++ GK+
Sbjct: 392 LIELQNKISLEKSAE-LRGKIVEVLIEGISKRDSNKLTSRTRTNKVVHFVGDESLIGKLA 450
Query: 417 NVKINSFEGEVAEGEIV 433
NVKI + +GE+V
Sbjct: 451 NVKITETKAWTMQGELV 467
>MIAB_THEPX RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 471
Score = 255 bits (651), Expect = 6e-70
Identities = 164/437 (37%), Positives = 254/437 (58%), Gaps = 16/437 (3%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIGRA--- 63
T GC++N ++SE + + GY+ + E ADV + NTC+V + + ++GR
Sbjct: 37 TYGCQMNVHDSEKLAGMLEKMGYKYTENLEQADVLLFNTCAVREHAEIR---VLGRVSQM 93
Query: 64 ---RRQNPEAIIAVVGCYAQ---IAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGKQ 117
+ +NP II V GC Q + +D+V G+ N + + +A +
Sbjct: 94 KELKARNPNLIIGVSGCMMQEKNVVEAIKEKYSYIDIVFGTHNIYKFPQLLWEALNSQDI 153
Query: 118 QVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVL 177
+ + KN V E+L ++ +N +A++ I GCN FC YC++PYTRG S+ P+ ++
Sbjct: 154 VIDIIEDTKN-VIEELPVKRDSN-LKAWVNIIYGCNNFCTYCIVPYTRGREKSRKPEDII 211
Query: 178 DEIRSLAEHGFKEIILSGIHTASYGVDLDEKVTLVDLLEEIEKIDGIERVRIGSIDPTFF 237
E++ LA+ G+KEI L G + SYG DLDE +T LL ++ I+GIER+R + P
Sbjct: 212 AEVKELAQKGYKEITLLGQNVNSYGKDLDEDITFAKLLYKLNDIEGIERIRFMTSHPKDI 271
Query: 238 TEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITT 297
+++++ + L K+C H HL +Q+G + LK+MNR+YT + Y +I++ +R I D++ITT
Sbjct: 272 SDELIYAMRDLDKVCEHLHLPVQAGSNKILKKMNRKYTKEHYLEIIDKVRSNIPDIAITT 331
Query: 298 DVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSKK 357
D+IVGFPG R+ + F YS R GT A NM +QVD K ER ++
Sbjct: 332 DIIVGFPGXXXXXXXXXXXXXXRVRFDAAYTFIYSKRAGTVAANMPDQVDDAVKHERLER 391
Query: 358 LIEL-NRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSGKIL 416
LIEL N+I+ + AE G++++VL E + S T TR V+ D ++ GK+
Sbjct: 392 LIELQNKISLEKSAE-LRGKIVEVLIEGISKRDSNKLTSRTRTNKVVHFVGDESLIGKLA 450
Query: 417 NVKINSFEGEVAEGEIV 433
NVKI + +GE+V
Sbjct: 451 NVKITETKAWTMQGELV 467
>MIAB_PELTS RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 448
Score = 253 bits (646), Expect = 2e-69
Identities = 147/425 (34%), Positives = 222/425 (52%), Gaps = 7/425 (1%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIG 61
K GC++N ++SE + GY +E D+ ++NTC V + K ++G
Sbjct: 3 KYRIIVFGCQMNEHDSEVLAGILESMGYCQAGNSEDPDIILINTCCVRKTAENKVFSLLG 62
Query: 62 RARRQ---NPEAIIAVVGCYAQ---IAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEG 115
R RRQ NP II V GC Q +A VD++ G+ N ++ + K +
Sbjct: 63 RLRRQKAQNPNLIIGVCGCMPQQEGMAERIKQLFPHVDLIFGTHNVHQLPELIGKVIEGQ 122
Query: 116 KQQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKK 175
KQ +++ ++ E+L ++ RA++ I GCN FC YC++PY RG S+ P+
Sbjct: 123 KQVLEIWPGYGGELREELPVKRKEG-VRAWVTIMYGCNNFCTYCIVPYVRGREKSRSPEA 181
Query: 176 VLDEIRSLAEHGFKEIILSGIHTASYGVDLDEKVTLVDLLEEIEKIDGIERVRIGSIDPT 235
V +E+ LA GFKE+IL G + SYG DL K LLE +E IDGI+R+R + P
Sbjct: 182 VYEEVARLAGEGFKEVILLGQNVNSYGKDLGVKTDFASLLESLENIDGIDRIRYMTSHPR 241
Query: 236 FFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSI 295
F+ +V I A KK+C HFHL +Q+G + LK+MNR YT +EY D++ ++ I ++
Sbjct: 242 DFSLRLVEAIAASKKVCEHFHLPVQAGSNRILKKMNRGYTREEYVDLIRYIKSLIPHATV 301
Query: 296 TTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERS 355
TTD++VGFPG ++ I + F Y+ R GT A M +QV N K ER
Sbjct: 302 TTDIMVGFPGETDEDFNDTLDLVREIRFDSAYTFVYNIRPGTPAAEMPDQVAENVKKERI 361
Query: 356 KKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSGKI 415
+ LI+L E+ +G+ +VL E E + GSG G R V D ++ GK+
Sbjct: 362 QALIKLQNKISLERNEEEVGQTQEVLVEGEKDRGSGFIYGRNRGNKTVIFSGDPSLVGKV 421
Query: 416 LNVKI 420
+ V +
Sbjct: 422 VPVTV 426
>MIAB_CLOBH RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 450
Score = 252 bits (644), Expect = 4e-69
Identities = 152/427 (35%), Positives = 230/427 (53%), Gaps = 13/427 (3%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKK---SRQIIGRA 63
T GC++N +SE ++ +EGY + E ADV + NTC V + K + I+
Sbjct: 20 TWGCQMNEEDSEKLSGMLKKEGYIRTEERENADVIIFNTCCVRENAELKVYGNLGILKGL 79
Query: 64 RRQNPEAIIAVVGCYAQ---IAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVK 120
+ +NP IIAV GC Q +A T VD+++G+ N Y+N+ + + +K
Sbjct: 80 KSKNPNLIIAVTGCMMQQKGMAETIKKKFPFVDIIIGTHNLHNFPNYLNEVKKKDTSVLK 139
Query: 121 VGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDEI 180
+ +N + E++ I+ N +AF+ I GCN FC YC++PY RG S+ P+ + EI
Sbjct: 140 IQEK-ENSIIENMPIDR-KNSMKAFVTIMYGCNNFCTYCIVPYVRGRERSRTPENIEAEI 197
Query: 181 RSLAEHGFKEIILSGIHTASYGVDLDEKVTLVDLLEEIEKIDGIERVRIGSIDPTFFTED 240
+ L G+KEI L G + SYG DL+ VT +LL+ + I+G+ERVR + P T+D
Sbjct: 198 KKLISEGYKEITLLGQNVNSYGKDLEPNVTFAELLKRVNNIEGLERVRFMTSHPKDLTDD 257
Query: 241 VVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITTDVI 300
V+ I KLC HL +QSG LK+MNR Y ++Y D+V+ ++ I +V+++TD+I
Sbjct: 258 VIEAIAKCDKLCEQIHLPVQSGSSEILKKMNRHYDREKYLDVVSKIKKLIPNVALSTDII 317
Query: 301 VGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSKKLIE 360
VGFPG +K + F YS RKGT A E+QV + K +R +L+E
Sbjct: 318 VGFPGETEKDFEETLSLVKEVEYDSAFTFLYSIRKGTPAAKFEDQVPEDVKHKRFNRLVE 377
Query: 361 -LNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSGKILNVK 419
LN I+ K + Y G++ +VL E + G TR VN D + G+++NVK
Sbjct: 378 VLNEISAKK-NKAYEGKIEEVLVEGTSKNDENKLMGRTRTGKLVNFIGDKDSIGELVNVK 436
Query: 420 I---NSF 423
I NSF
Sbjct: 437 IIKANSF 443
>MIAB_CLOB1 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 450
Score = 252 bits (644), Expect = 4e-69
Identities = 152/427 (35%), Positives = 230/427 (53%), Gaps = 13/427 (3%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKK---SRQIIGRA 63
T GC++N +SE ++ +EGY + E ADV + NTC V + K + I+
Sbjct: 20 TWGCQMNEEDSEKLSGMLKKEGYIRTEERENADVIIFNTCCVRENAELKVYGNLGILKGL 79
Query: 64 RRQNPEAIIAVVGCYAQ---IAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVK 120
+ +NP IIAV GC Q +A T VD+++G+ N Y+N+ + + +K
Sbjct: 80 KSKNPNLIIAVTGCMMQQKGMAETIKKKFPFVDIIIGTHNLHNFPNYLNEVKKKDTSVLK 139
Query: 121 VGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDEI 180
+ +N + E++ I+ N +AF+ I GCN FC YC++PY RG S+ P+ + EI
Sbjct: 140 IQEK-ENSIIENMPIDR-KNSMKAFVTIMYGCNNFCTYCIVPYVRGRERSRTPENIEAEI 197
Query: 181 RSLAEHGFKEIILSGIHTASYGVDLDEKVTLVDLLEEIEKIDGIERVRIGSIDPTFFTED 240
+ L G+KEI L G + SYG DL+ VT +LL+ + I+G+ERVR + P T+D
Sbjct: 198 KKLISEGYKEITLLGQNVNSYGKDLEPNVTFAELLKRVNNIEGLERVRFMTSHPKDLTDD 257
Query: 241 VVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITTDVI 300
V+ I KLC HL +QSG LK+MNR Y ++Y D+V+ ++ I +V+++TD+I
Sbjct: 258 VIEAIAKCDKLCEQIHLPVQSGSSEILKKMNRHYDREKYLDVVSKIKKLIPNVALSTDII 317
Query: 301 VGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSKKLIE 360
VGFPG +K + F YS RKGT A E+QV + K +R +L+E
Sbjct: 318 VGFPGETEKDFEETLSLVKEVEYDSAFTFLYSIRKGTPAAKFEDQVPEDVKHKRFNRLVE 377
Query: 361 -LNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSGKILNVK 419
LN I+ K + Y G++ +VL E + G TR VN D + G+++NVK
Sbjct: 378 VLNEISAKK-NKAYEGKIEEVLVEGTSKNDENKLMGRTRTGKLVNFIGDKDSIGELVNVK 436
Query: 420 I---NSF 423
I NSF
Sbjct: 437 IIKANSF 443
>MIAB_CLOBL RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 450
Score = 248 bits (634), Expect = 5e-68
Identities = 150/427 (35%), Positives = 229/427 (53%), Gaps = 13/427 (3%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKK---SRQIIGRA 63
T GC++N +SE ++ +EGY + E ADV + NTC V + K + I+
Sbjct: 20 TWGCQMNEEDSEKLSGMLKKEGYIRTEERENADVIIFNTCCVRENAELKVYGNLGILKGL 79
Query: 64 RRQNPEAIIAVVGCYAQ---IAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVK 120
+ +NP IIAV GC Q +A T VD+++G+ N Y+N+ + + +K
Sbjct: 80 KSKNPNLIIAVTGCMMQQKGMAETIKKKFPFVDIIIGTHNLHNFPNYLNEVKKKDTSVLK 139
Query: 121 VGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDEI 180
+ ++ + E++ I+ N +AF+ I GCN FC YC++PY RG S+ P+ + EI
Sbjct: 140 IQEK-EDSIIENMPIDR-KNSMKAFVTIMYGCNNFCTYCIVPYVRGRERSRTPENIEAEI 197
Query: 181 RSLAEHGFKEIILSGIHTASYGVDLDEKVTLVDLLEEIEKIDGIERVRIGSIDPTFFTED 240
+ L G+KEI L G + SYG DL+ VT +LL+ + I+G+ERVR + P T+D
Sbjct: 198 KKLISEGYKEITLLGQNVNSYGKDLEPNVTFAELLKRVNNIEGLERVRFMTSHPKDLTDD 257
Query: 241 VVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITTDVI 300
V+ I KLC HL +QSG LK+MNR Y ++Y D+V+ ++ I +V+++TD+I
Sbjct: 258 VIEAIAKCDKLCEQIHLPVQSGSSEILKKMNRHYDREKYLDVVSKIKKLIPNVALSTDII 317
Query: 301 VGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSKKLIE 360
VGFPG +K + F YS RKGT A E+QV + K +R +L+E
Sbjct: 318 VGFPGETEKDFEETLSLVKEVEYDSAFTFLYSIRKGTPAAKFEDQVPEDVKHKRFNRLVE 377
Query: 361 -LNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSGKILNVK 419
+N I+ K + Y G++ +VL E + G TR VN D + G+ +NVK
Sbjct: 378 VVNEISAKK-NKAYEGKIEEVLVEGTSKNDENKLMGRTRTGKLVNFIGDKDSIGEFVNVK 436
Query: 420 I---NSF 423
I NSF
Sbjct: 437 IIKANSF 443
>MIAB_CLOB8 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 455
Score = 246 bits (629), Expect = 2e-67
Identities = 145/432 (33%), Positives = 228/432 (52%), Gaps = 13/432 (3%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIGRARRQ 66
T GC++N +SE ++ R GYE + + A + + NTC V + K +G ++Q
Sbjct: 24 TYGCQMNEEDSEKLSGMLKRMGYENTENRDEASIIIFNTCCVRENAENKVFGNLGALKKQ 83
Query: 67 ---NPEAIIAVVGCYAQ---IAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVK 120
NP+ +I + GC Q +A + V+++ G+ N + Y+N+ + EG Q +K
Sbjct: 84 KEKNPDLVIGICGCMMQQKGMADDILKRFPYVNIIFGTHNSYKFPEYLNRVKTEGVQ-IK 142
Query: 121 VGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDEI 180
+ ++ E + I+ ++ + F+ I GCN FC YC++PY RG S+ P+ +++EI
Sbjct: 143 EIIDKETEIVEGIPIDRKSD-IKGFVTIMYGCNNFCTYCIVPYVRGRERSRKPEDIVNEI 201
Query: 181 RSLAEHGFKEIILSGIHTASYGVDLDEKVTLVDLLEEIEKIDGIERVRIGSIDPTFFTED 240
+ + G+KE+ L G + SYG L+E +T DLL ++ +I+G+ER+R + P T D
Sbjct: 202 KDMVTRGYKEVTLLGQNVNSYGKGLEENITFADLLRKVNEIEGLERIRFMTSHPKDLTLD 261
Query: 241 VVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITTDVI 300
VV I K+C HL +QSG D LK MNR YT ++Y + +R +I DV+ +TD+I
Sbjct: 262 VVYAIRDCDKVCEQIHLPVQSGSDRILKEMNRHYTKEQYITLAKKIRAEIPDVTFSTDII 321
Query: 301 VGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSKKLIE 360
VGFPG K + F YS R T A+ MENQ+ K ER +L+E
Sbjct: 322 VGFPGETEEDFSETLELAKEVRYDAAFTFIYSRRNHTPADKMENQIPDEIKHERFNRLVE 381
Query: 361 LNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSGKILNVKI 420
+ + G++ +VL E + TG TRN VN + ++ GK++NVKI
Sbjct: 382 IVNTGIAKGNKDAEGKIYEVLVEGYSKNDEAKLTGRTRNGRLVNFEGGEDLIGKLVNVKI 441
Query: 421 ---NSFE--GEV 427
NSF GEV
Sbjct: 442 IKANSFSLIGEV 453
>MIAB_THETN RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 471
Score = 244 bits (623), Expect = 1e-66
Identities = 153/437 (35%), Positives = 235/437 (53%), Gaps = 16/437 (3%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIGRA--- 63
T GC++N ++SE + GY+ + E ADV + NTC+V + + ++GR
Sbjct: 37 TYGCQMNVHDSEKLAGMLEEMGYKYTEDLEKADVLLFNTCAVREHAEVR---VLGRVSQI 93
Query: 64 ---RRQNPEAIIAVVGCYAQ---IAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGKQ 117
+ +NP II + GC Q I VD+V G+ N + + +A + Q
Sbjct: 94 KELKNRNPNLIIGISGCMMQEKHIVEAIREKYPHVDIVFGTHNIYKFPELLWQALNSRVQ 153
Query: 118 QVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVL 177
+ V +N V E L I +N +A++ I GCN FC YC++PYTRG S+ P+ ++
Sbjct: 154 VIDVIENTQN-VIEGLPIRRDSN-LKAWVNIIYGCNNFCTYCIVPYTRGREKSRRPEDII 211
Query: 178 DEIRSLAEHGFKEIILSGIHTASYGVDLDEKVTLVDLLEEIEKIDGIERVRIGSIDPTFF 237
E++ LAE G+KEI L G + SYG DL + LL + I GIER+R + P
Sbjct: 212 AEVKELAEKGYKEITLLGQNVNSYGKDLGGDINFAKLLYMLNDIKGIERIRFMTSHPKDI 271
Query: 238 TEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITT 297
+++++ + L K+C H HL +Q+G + LKRMNRRYT + Y +I++ +R I D++ITT
Sbjct: 272 SDELIYAMRDLDKVCEHLHLPVQAGSNKILKRMNRRYTREHYLEIIDKVRANIPDIAITT 331
Query: 298 DVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSKK 357
D+IVGFPG ++R+ + F YS R GT A +M +QVD K +R +
Sbjct: 332 DIIVGFPGETEEDFFQTLDLVERVRFDGAYTFMYSKRAGTPAASMPDQVDDEVKKDRLIR 391
Query: 358 LIEL-NRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSGKIL 416
LIEL N+I+ + AE G+V++VL E + T TR V+ D ++ GK+
Sbjct: 392 LIELQNKISLEKSAE-LKGKVVEVLIEGVSKRDPNKLTSRTRTNKVVHFIGDESLIGKLA 450
Query: 417 NVKINSFEGEVAEGEIV 433
NVKI + +GE+V
Sbjct: 451 NVKITETKAWTMQGELV 467
>MIAB_CLOTH RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 480
Score = 242 bits (618), Expect = 4e-66
Identities = 140/434 (32%), Positives = 223/434 (51%), Gaps = 10/434 (2%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIGRAR-- 64
T GC++N ++SE + GY D +D+ + NTC V + K +G +
Sbjct: 49 TFGCQMNEHDSEKLAGMLAEMGYAETDNVNESDLVIYNTCCVRENAELKVYGHLGMLKPL 108
Query: 65 -RQNPEAIIAVVGCYAQIAPTEVSGIEG----VDVVLGSRNKGEIVYYVNKARDEGKQQV 119
Q P+ +IAV GC Q P V I+ VD++ G+ N + + A D V
Sbjct: 109 KNQKPDLVIAVCGCMMQ-QPEVVEHIKKTYSHVDLIFGTHNLYKFPELLYSAMDSQTTVV 167
Query: 120 KVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDE 179
V ++ E++ IE + +A++ + GCN FC YC++PY RG S+ +L+E
Sbjct: 168 DVWDC-DGQIAENVAIERKDG-VKAWVTVMYGCNNFCTYCIVPYVRGRERSRSMDDILEE 225
Query: 180 IRSLAEHGFKEIILSGIHTASYGVDLDEKVTLVDLLEEIEKIDGIERVRIGSIDPTFFTE 239
+R L GFKEI L G + SYG D+ + + +L+ E+ KI GIER+R + P ++
Sbjct: 226 VRMLGRQGFKEITLLGQNVNSYGKDIGDGTSFAELIREVNKIPGIERIRFTTSHPKDLSD 285
Query: 240 DVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITTDV 299
D++ + +K+C H HL Q+G LK MNR+YT ++Y ++V +++ I D+++TTD+
Sbjct: 286 DLIYAMRDCEKVCEHLHLPFQAGSTRILKLMNRKYTKEDYINLVAKIKENIPDIALTTDI 345
Query: 300 IVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSKKLI 359
IVGFPG L+++ + F YS R GT A ME+QV K ER ++L+
Sbjct: 346 IVGFPGETEEDFSDTLDILEKVRFDNAYTFLYSKRTGTPAAKMEDQVPEEVKKERFQRLL 405
Query: 360 ELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSGKILNVK 419
E K + + ++TG TR VN +AD ++ GK++NV+
Sbjct: 406 ETQNRISKEINDTFXXXXXXXXXXXXXXTNDKIFTGRTRGNKVVNFEADASLIGKLVNVR 465
Query: 420 INSFEGEVAEGEIV 433
IN+ + EG IV
Sbjct: 466 INTVKTWSLEGSIV 479
>MIAB_CLOBB RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 456
Score = 242 bits (618), Expect = 4e-66
Identities = 146/433 (33%), Positives = 226/433 (52%), Gaps = 8/433 (1%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIGRARRQ 66
T GC++N +SE ++ +GYE + + A + + NTC V + K +GR + Q
Sbjct: 25 TWGCQMNEEDSEKLSGMLKSQGYEETENRDEASIVIFNTCCVRENAENKVFGNLGRLKNQ 84
Query: 67 ---NPEAIIAVVGCYAQ---IAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVK 120
NP IIA+ GC Q +A +S VD++ G+ N + Y+++ + EG Q VK
Sbjct: 85 KEKNPNLIIALCGCMMQQKGMADEILSRFPYVDIIFGTHNAYKFPEYLHRVQVEGVQ-VK 143
Query: 121 VGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDEI 180
+ ++ E + I+ +N +AF+ I GCN FC YC++PY RG S+ P+ + +EI
Sbjct: 144 EIFDKETEIVEGVPIDRKSN-VKAFVTIMYGCNNFCTYCVVPYVRGRERSRRPEDIENEI 202
Query: 181 RSLAEHGFKEIILSGIHTASYGVDLDEKVTLVDLLEEIEKIDGIERVRIGSIDPTFFTED 240
+ L G+KEI L G + SYG L+E++T LL I +IDG+ER+R + P T D
Sbjct: 203 KELVSSGYKEITLLGQNVNSYGKGLEEEITFAQLLRRINEIDGLERLRFMTSHPKDLTLD 262
Query: 241 VVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITTDVI 300
VV I KLC HL +QSG + L++MNR Y ++Y ++ +R++I DV+ +TD+I
Sbjct: 263 VVYAIRDCDKLCEQIHLPVQSGSNEILQKMNRHYNKEQYLELAKKIREEIPDVTFSTDII 322
Query: 301 VGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSKKLIE 360
VGFPG +K + F YS R T A+ MENQ+ + K +R +L+
Sbjct: 323 VGFPGETEEDFEETINLVKEVRYDAAFTFIYSRRNHTPADKMENQIPDDVKHDRFNRLVA 382
Query: 361 LNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSGKILNVKI 420
+ G++ +VL E + TG TRN VN + GK++ VKI
Sbjct: 383 AVNEGIVVGNKAAEGKIYEVLVEGTSKNNENKLTGRTRNAKLVNFDGCKEMIGKLVKVKI 442
Query: 421 NSFEGEVAEGEIV 433
+ GE+V
Sbjct: 443 IEAKSFSLVGEVV 455
>MIAB_CLOBA RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 456
Score = 242 bits (617), Expect = 5e-66
Identities = 145/433 (33%), Positives = 227/433 (52%), Gaps = 8/433 (1%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIGRARRQ 66
T GC++N +SE ++ +GYE + + A + + NTC V + K +GR + Q
Sbjct: 24 TFGCQMNEEDSEKLSGMLKSQGYEETENRDEASIVIFNTCCVRENAENKVFGNLGRLKNQ 83
Query: 67 ---NPEAIIAVVGCYAQ---IAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVK 120
NP IIA+ GC Q +A +S VD++ G+ N + Y+++ + EG Q VK
Sbjct: 84 KEKNPNLIIALCGCMMQQKGMADEILSRFPYVDIIFGTHNAYKFPEYLHRVQVEGVQ-VK 142
Query: 121 VGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDEI 180
+ ++ E + I+ +N +AF+ I GCN FC YC++PY RG S+ P+ + +EI
Sbjct: 143 EIFDKETEIVEGVPIDRKSN-VKAFVTIMYGCNNFCTYCVVPYVRGRERSRRPEDIENEI 201
Query: 181 RSLAEHGFKEIILSGIHTASYGVDLDEKVTLVDLLEEIEKIDGIERVRIGSIDPTFFTED 240
+ L +G+KEI L G + SYG L+E++T LL I +IDG+ER+R + P T D
Sbjct: 202 KELVSNGYKEITLLGQNVNSYGKGLEEEITFAQLLRRINEIDGLERLRFMTSHPKDLTLD 261
Query: 241 VVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITTDVI 300
VV I KLC HL +QSG + L++MNR Y ++Y ++ +R++I DV+ +TD+I
Sbjct: 262 VVYAIRDCDKLCEQIHLPVQSGSNEILQKMNRHYNKEQYLELAKKIREEIPDVTFSTDII 321
Query: 301 VGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSKKLIE 360
VGFPG +K + F YS R T A+ ME+Q+ + K +R +L+
Sbjct: 322 VGFPGETEEDFEETINLVKEVRYDAAFTFIYSRRNHTPADKMEDQIPDDVKHDRFNRLVA 381
Query: 361 LNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSGKILNVKI 420
+ G++ +VL E + TG TRN VN + GK++ VKI
Sbjct: 382 AVNEGIVVGNKAAEGKIYEVLVEGTSKNNENKLTGRTRNAKLVNFDGCKEMIGKLVKVKI 441
Query: 421 NSFEGEVAEGEIV 433
+ GE+V
Sbjct: 442 IEAKSFSLVGEVV 454
>MIAB_CLONN RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 452
Score = 241 bits (614), Expect = 1e-65
Identities = 139/435 (31%), Positives = 231/435 (53%), Gaps = 10/435 (2%)
Query: 6 ATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIGRAR- 64
+T GC++N +SE ++ GY D + ADV + NTC V ++K +G +
Sbjct: 21 STWGCQMNEEDSEKISGLLKGIGYTRTDIRDEADVVIFNTCCVRENAEQKVYGHLGELKA 80
Query: 65 --RQNPEAIIAVVGCYAQ---IAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQV 119
R+NP I+ V GC Q + + VD++ G+ N ++ Y+ + + EG +
Sbjct: 81 LKRKNPNLILIVTGCMMQQKGMPEKVMEKFPHVDIIAGTYNSYKLPEYIERVKTEGNSII 140
Query: 120 KVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDE 179
++ K + E L ++ ++ +AF+ I GCN FC+YC++PY RG S+DP+ ++DE
Sbjct: 141 EIWDKEKG-IVEGLPVDRKSD-IKAFVTIMYGCNNFCSYCIVPYVRGRERSRDPQNIIDE 198
Query: 180 IRSLAEHGFKEIILSGIHTASYGVDLDEKVTLVDLLEEIEKIDGIERVRIGSIDPTFFTE 239
I+ L G+KEI L G + SYG L+ ++ LL + KIDG+ER+R + P ++
Sbjct: 199 IKDLVSKGYKEITLLGQNVNSYGKGLEPEINFATLLRMVNKIDGLERIRFMTSHPKDVSD 258
Query: 240 DVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITTDV 299
++++ + +K+C H +LQSG L++MNR+YT ++Y +V LR + +V I+TD+
Sbjct: 259 ELIKAMAECEKVCEQGHFALQSGSTEILQKMNRKYTREDYLTLVKKLRKAMPNVGISTDI 318
Query: 300 IVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSKKLI 359
I+G+PG +K I F YS R+GT A +E+QV + K R KL+
Sbjct: 319 IIGYPGETEKDFEDTLSIVKEIEFDSAFTFIYSKREGTPAAKLEDQVPEDVKHTRFNKLV 378
Query: 360 E-LNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSGKILNV 418
E +N I + E + G+ ++VL E + G TR+ VN + GK++N+
Sbjct: 379 EAVNEIMARKNKE-FEGKTVEVLVEGPSKNDDTKLMGRTRSGKLVNFNGCLDQVGKLVNI 437
Query: 419 KINSFEGEVAEGEIV 433
KI GEI+
Sbjct: 438 KITKANSFSLTGEII 452
>MIAB_ALKOO RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 471
Score = 239 bits (610), Expect = 3e-65
Identities = 147/441 (33%), Positives = 226/441 (51%), Gaps = 14/441 (3%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIG 61
K T GC++N ++SE ++ GY + E A++ + NTC V + K IG
Sbjct: 34 KYMITTYGCQMNEHDSETLSGMLENMGYSITTNKEEANLIIYNTCCVRENAELKVYGNIG 93
Query: 62 RAR---RQNPEAIIAVVGCYAQIAPTEVSGIEG----VDVVLGSRNKGEIVYYVNKARDE 114
+ ++N + IIAV GC Q P V I+ VD+V G+ N ++++ +
Sbjct: 94 ALKALKKKNEDLIIAVCGCMMQ-QPQVVKEIKRKYRHVDLVFGTHNLYRFPELLSRSMET 152
Query: 115 GKQQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPK 174
++V + + E L + + F+ I GCN FC YC++PYTRG S++
Sbjct: 153 EGMFIEVWDE-ETGIVEGLPANRKYD-LKGFINIMYGCNNFCTYCIVPYTRGRERSREVA 210
Query: 175 KVLDEIRSLAEHGFKEIILSGIHTASYGVDLDEKVTLVDLLEEIEKIDGIERVRIGSIDP 234
++ E LA +G KEI L G + SYG L+ + DLL + KIDGIER+R + P
Sbjct: 211 DIIREATDLANNGTKEITLLGQNVNSYGKTLEHPIDFADLLRALNKIDGIERIRFMTSHP 270
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
+E ++ I K+C HFHL QSG + LK MNR+YT + Y IV L+D+I ++
Sbjct: 271 KDLSERLIDAIAECDKVCEHFHLPFQSGSNQILKAMNRKYTKENYLSIVKKLKDRIPNIG 330
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
+TTD+IVGFPG ++ + F YS R+GT A M+NQ+D K ER
Sbjct: 331 LTTDIIVGFPGETEEDFQDTLDIVQEARYDSAYTFLYSIREGTPAAKMQNQIDEKVKQER 390
Query: 355 SKKLIELNRINE--KAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVS 412
+L L+++NE + Y+ +V++VL E + S G TR VN D ++
Sbjct: 391 FSRL--LDKVNEISAEINQSYLNKVVEVLVEGPSKTDSNKLMGRTRQNKLVNFSGDESLI 448
Query: 413 GKILNVKINSFEGEVAEGEIV 433
GK++NV+I GE++
Sbjct: 449 GKLVNVRIVECRTFSLNGEVI 469
>MIAB_CLOAB RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 441
Score = 238 bits (608), Expect = 6e-65
Identities = 149/435 (34%), Positives = 225/435 (51%), Gaps = 12/435 (2%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIGRARR- 65
T GC++N +SE + YE D E AD+ + NTC V + K +G ++
Sbjct: 11 TWGCQMNAEDSEKLAGMLKEMKYEATDNREDADLIIFNTCCVRENAELKVYGNLGTLKKL 70
Query: 66 --QNPEAIIAVVGCYAQ---IAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVK 120
+ P II V GC Q +A VD+V+G+ N Y+ K +E V+
Sbjct: 71 KDKKPNLIITVCGCMMQQRDMAEHIKKRFPFVDIVMGTHNTQMFPQYLKKVENERTSVVE 130
Query: 121 VGAVLKNKVFEDLKI-EEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDE 179
+ + + E + I Y+ K AF+ I GCN FC YC++PY RG S+ P+ + +E
Sbjct: 131 IWDK-EEGIVEGMPIYRSYDMK--AFVTIMYGCNNFCTYCIVPYVRGRERSRKPEDIENE 187
Query: 180 IRSLAEHGFKEIILSGIHTASYGVDLDEKVTLVDLLEEIEKIDGIERVRIGSIDPTFFTE 239
I+ L + G+KEI L G + SYG LDE++ LL + I+G+ R+R + P T+
Sbjct: 188 IKELVKKGYKEITLLGQNVNSYGKSLDEEMNFALLLRRLNNIEGLLRIRFMTSHPKDLTD 247
Query: 240 DVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITTDV 299
DV+ I K+C H HL +QSG L +MNR YT ++Y ++VN ++ I++V+ITTD+
Sbjct: 248 DVIAAIADCDKVCEHIHLPVQSGSTTILNKMNRNYTREDYINLVNKIKSGIKNVAITTDI 307
Query: 300 IVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSKKLI 359
IVGFPG +K + F YS R+GT A ENQ+ K +R KL+
Sbjct: 308 IVGFPGETEEDFEDTLSLVKEVEYDSAFTFLYSIREGTPAAKYENQIPDEVKHKRFNKLL 367
Query: 360 E-LNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSGKILNV 418
E +N I+EK E Y G+++++L E + + G TR VN + + GK++NV
Sbjct: 368 EAVNLISEKKNKE-YEGKIVEILVEGKSKNDGSKLMGRTRTGKLVNFEGLESSIGKLINV 426
Query: 419 KINSFEGEVAEGEIV 433
KI + GE V
Sbjct: 427 KITKAQAFSLVGEEV 441
>MIAB_NATTJ RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 451
Score = 237 bits (605), Expect = 1e-64
Identities = 141/432 (32%), Positives = 225/432 (52%), Gaps = 8/432 (1%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIGRAR-- 64
T GC++N ++SE + + G+E E AD+ ++NTC+V ++K IG R
Sbjct: 16 TFGCQMNEHDSEVLAGMLDQMGFEKAASEEEADLLIINTCAVREKAEQKVLGKIGTLRYL 75
Query: 65 -RQNPEAIIAVVGCYAQ---IAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVK 120
P+ IA+ GC Q +A VD++ G+ N + +GK+ VK
Sbjct: 76 KENKPDMKIAIGGCMVQQEHVANKIYRDFTHVDIIFGTHNINRFPQLLEHVMQKGKR-VK 134
Query: 121 VGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDEI 180
+ ++VFE+L + + +A++ I GC+ +C YC++PY RG S+DP+ + E+
Sbjct: 135 EISQDDSQVFENLP-HKREDSIKAWVVISYGCDNYCKYCIVPYVRGQQRSRDPEHIKYEV 193
Query: 181 RSLAEHGFKEIILSGIHTASYGVDLDEKVTLVDLLEEIEKIDGIERVRIGSIDPTFFTED 240
LA+ G KEI L G + SYG DLD+ ++ +LLEE+ KI+GIER+R + P F ++
Sbjct: 194 EKLAKEGLKEITLLGQNVNSYGKDLDQNISFTNLLEELSKIEGIERIRFMTSHPKDFDKE 253
Query: 241 VVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITTDVI 300
++ + K+C HFHL +Q+G + LK+M R YT + Y DIVN +R ++ + SITTD+I
Sbjct: 254 LITTLKESNKICEHFHLPVQAGSNKILKKMGRGYTREHYVDIVNDIRAELPNASITTDII 313
Query: 301 VGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSKKLIE 360
VG+PG ++ + F YS R GT A M QVD K R +KLI
Sbjct: 314 VGYPGEEEEDFQETLDLVQNVKFDSAFTFVYSKRSGTPAAEMAEQVDEQTKKGRIQKLIS 373
Query: 361 LNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSGKILNVKI 420
+ + + + V +L E + + +G TR V+ D + G++++VKI
Sbjct: 374 VQQEISEQRNKDLENTVQRILVEGVSKNNEDMLSGRTRTDKLVHFPGDKELIGELVDVKI 433
Query: 421 NSFEGEVAEGEI 432
GEI
Sbjct: 434 TRGHSWNLYGEI 445
>MIAB_CLOPH RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 456
Score = 233 bits (594), Expect = 2e-63
Identities = 139/436 (31%), Positives = 233/436 (53%), Gaps = 15/436 (3%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQ---IIGRA 63
T GC++N +SE + GY D +E AD V NTC+V + + +G
Sbjct: 25 TFGCQMNAKDSEKLAGILETIGYVESD-SEEADFVVYNTCTVRENANTRVYGRIGFLGNL 83
Query: 64 RRQNPEAIIAVVGCYAQ---IAPTEVSGIEGVDVVLGSRN---KGEIVYYVNKARDEGKQ 117
+++NP IA+ GC Q + VD+V G+ N E++Y AR K+
Sbjct: 84 KKKNPHMRIALCGCMMQESHVVEKIKKSYRFVDIVFGTHNIFKLAELIY----ARHTTKK 139
Query: 118 QVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVL 177
V +K+ E+L E+ K +A + I GCN FC+YC++PY RG S++P+ ++
Sbjct: 140 MVIDIWKETDKIVEELPSEQ-KYKFKAGVNIMYGCNNFCSYCIVPYVRGRERSRNPEDII 198
Query: 178 DEIRSLAEHGFKEIILSGIHTASYGVDLDEKVTLVDLLEEIEKIDGIERVRIGSIDPTFF 237
EI+ L G E++L G + SYG LDE V+ LL+ +E+++G++R+R + P
Sbjct: 199 KEIKQLVSKGVVEVMLLGQNVNSYGKTLDEPVSFAQLLQMVEQVEGLKRIRFMTPHPKDL 258
Query: 238 TEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITT 297
+ DV+ + KK+C H HL +QSG L +MNR+YT ++Y ++V+ ++ + ++S+TT
Sbjct: 259 SNDVIEVMKNSKKICNHIHLPVQSGSTELLMKMNRKYTKEDYLNLVDRIKMAMPNISLTT 318
Query: 298 DVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSKK 357
D+IVGFPG ++++ + F YS R GT A MENQ+ +ER ++
Sbjct: 319 DIIVGFPGETEEDFLDTLDVVRKVGYDSAYTFIYSKRSGTPAATMENQIPEEVANERFQR 378
Query: 358 LIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSGKILN 417
L+ R + ++ G++ +VL EE E + + TG N + V+ K + G I++
Sbjct: 379 LLTTIRESSSKISKDDEGKIAEVLVEEVNEQDNSLMTGRLSNNVLVHFKGTKELIGNIVS 438
Query: 418 VKINSFEGEVAEGEIV 433
VK++ +G GE++
Sbjct: 439 VKLSECKGFYYMGEMM 454
>MIAB_CLOPE RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 447
Score = 231 bits (590), Expect = 7e-63
Identities = 142/436 (32%), Positives = 221/436 (50%), Gaps = 12/436 (2%)
Query: 6 ATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKK---SRQIIGR 62
+T GC++N +SE ++ +GYE + E A + + NTC V + K
Sbjct: 15 STYGCQMNEEDSEKLSGMLKSQGYERTENKEEASIIIFNTCCVRENAENKVFXXXXXXXX 74
Query: 63 ARRQNPEAIIAVVGCYAQ---IAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQV 119
+IA+ GC Q +A + VD++ G+ N + Y+++ EG Q V
Sbjct: 75 XXXXXXXXVIAICGCMMQQVGMADKVLKTFPYVDIIFGTHNAHKFPEYLHRVLQEGVQ-V 133
Query: 120 KVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDE 179
K + + E L I+ ++ +AF+ I GCN FC YC++PY RG S+ + ++ E
Sbjct: 134 KEILNKEEGIVEGLPIDRKSD-VKAFVTIMYGCNNFCTYCIVPYVRGRERSRKSEDIIKE 192
Query: 180 IRSLAEHGFKEIILSGIHTASYGVDLDEKVTLVDLLEEIEKIDGIERVRIGSIDPTFFTE 239
I L G+KEI L G + SYG L+E + LL ++ ++ G+ERVR + P ++
Sbjct: 193 IEELVSQGYKEITLLGQNVNSYGKGLEEDIDFAGLLRKVNEVKGLERVRFMTSHPKDLSD 252
Query: 240 DVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITTDV 299
DV+ I KLC HL +QSG LK MNR Y + Y D+V ++ +I DV++TTD+
Sbjct: 253 DVIMAIKECDKLCEQVHLPVQSGSSRILKEMNRHYDREYYLDLVKKIKSEIPDVTLTTDI 312
Query: 300 IVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSKKLI 359
I+GFPG + + F YS R T A+ MENQ+ + K +R +L+
Sbjct: 313 IIGFPGETEEDFLDTLSLCEEVGYDSAFTFIYSRRNHTPADKMENQIPDDIKHDRFNRLV 372
Query: 360 ELNRINEKAFA--EKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSGKILN 417
E IN+K ++Y G+V++VL E + TG TRN VN D + G+++N
Sbjct: 373 E--AINKKVVIKNKEYEGKVVEVLVEGPSKNDETKLTGRTRNGKLVNFAGDEKLVGELVN 430
Query: 418 VKINSFEGEVAEGEIV 433
+KI + GEIV
Sbjct: 431 LKIVRAQPFSLIGEIV 446
>MIAB_CLOP1 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 447
Score = 231 bits (590), Expect = 7e-63
Identities = 142/436 (32%), Positives = 221/436 (50%), Gaps = 12/436 (2%)
Query: 6 ATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKK---SRQIIGR 62
+T GC++N +SE ++ +GYE + E A + + NTC V + K
Sbjct: 15 STYGCQMNEEDSEKLSGMLKSQGYERTENKEEASIIIFNTCCVRENAENKVFXXXXXXXX 74
Query: 63 ARRQNPEAIIAVVGCYAQ---IAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQV 119
+IA+ GC Q +A + VD++ G+ N + Y+++ EG Q V
Sbjct: 75 XXXXXXXXVIAICGCMMQQVGMADKVLKTFPYVDIIFGTHNAHKFPEYLHRVLQEGVQ-V 133
Query: 120 KVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDE 179
K + + E L I+ ++ +AF+ I GCN FC YC++PY RG S+ + ++ E
Sbjct: 134 KEILNKEEGIVEGLPIDRKSD-VKAFVTIMYGCNNFCTYCIVPYVRGRERSRKSEDIIKE 192
Query: 180 IRSLAEHGFKEIILSGIHTASYGVDLDEKVTLVDLLEEIEKIDGIERVRIGSIDPTFFTE 239
I L G+KEI L G + SYG L+E + LL ++ ++ G+ERVR + P ++
Sbjct: 193 IEELVSQGYKEITLLGQNVNSYGKGLEEDIDFAGLLRKVNEVKGLERVRFMTSHPKDLSD 252
Query: 240 DVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITTDV 299
DV+ I KLC HL +QSG LK MNR Y + Y D+V ++ +I DV++TTD+
Sbjct: 253 DVIMAIKECDKLCEQVHLPVQSGSSRILKEMNRHYDREYYLDLVKKIKSEIPDVTLTTDI 312
Query: 300 IVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSKKLI 359
I+GFPG + + F YS R T A+ MENQ+ + K +R +L+
Sbjct: 313 IIGFPGETEEDFLDTLSLCEEVGYDSAFTFIYSRRNHTPADKMENQIPDDIKHDRFNRLV 372
Query: 360 ELNRINEKAFA--EKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSGKILN 417
E IN+K ++Y G+V++VL E + TG TRN VN D + G+++N
Sbjct: 373 E--AINKKVVIKNKEYEGKVVEVLVEGPSKNDETKLTGRTRNGKLVNFAGDEKLVGELVN 430
Query: 418 VKINSFEGEVAEGEIV 433
+KI + GEIV
Sbjct: 431 LKIVRAQPFSLIGEIV 446
>MIAB_CLOPS RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 447
Score = 231 bits (589), Expect = 9e-63
Identities = 142/436 (32%), Positives = 221/436 (50%), Gaps = 12/436 (2%)
Query: 6 ATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKK---SRQIIGR 62
+T GC++N +SE ++ +GYE + E A + + NTC V + K
Sbjct: 15 STYGCQMNEEDSEKLSGMLKSQGYERTENKEEASIIIFNTCCVRENAENKVFXXXXXXXX 74
Query: 63 ARRQNPEAIIAVVGCYAQ---IAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQV 119
+IA+ GC Q +A + VD++ G+ N + Y+++ EG Q V
Sbjct: 75 XXXXXXXXVIAICGCMMQQVGMADKVLKTFPYVDIIFGTHNAHKFPEYLHRVLQEGVQ-V 133
Query: 120 KVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDE 179
K + + E L I+ ++ +AF+ I GCN FC YC++PY RG S+ + ++ E
Sbjct: 134 KEILNKEEGIVEGLPIDRKSD-VKAFVTIMYGCNNFCTYCIVPYVRGRERSRKSEDIIKE 192
Query: 180 IRSLAEHGFKEIILSGIHTASYGVDLDEKVTLVDLLEEIEKIDGIERVRIGSIDPTFFTE 239
I L G+KEI L G + SYG L+E + LL ++ ++ G+ERVR + P ++
Sbjct: 193 IEELVSKGYKEITLLGQNVNSYGKGLEEDIDFAGLLRKVNEVKGLERVRFMTSHPKDLSD 252
Query: 240 DVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITTDV 299
DV+ I KLC HL +QSG LK MNR Y + Y D+V ++ +I DV++TTD+
Sbjct: 253 DVIMAIKECDKLCEQVHLPVQSGSSRILKEMNRHYDREYYLDLVKKIKSEIPDVTLTTDI 312
Query: 300 IVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSKKLI 359
I+GFPG + + F YS R T A+ MENQ+ + K +R +L+
Sbjct: 313 IIGFPGETEEDFLDTLSLCEEVGYDSAFTFIYSRRNHTPADKMENQIPDDIKHDRFNRLV 372
Query: 360 ELNRINEKAFA--EKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSGKILN 417
E IN+K ++Y G+V++VL E + TG TRN VN D + G+++N
Sbjct: 373 E--AINKKVVIKNKEYEGKVVEVLVEGPSKNDETKLTGRTRNGKLVNFAGDEKLVGELVN 430
Query: 418 VKINSFEGEVAEGEIV 433
+KI + GEIV
Sbjct: 431 LKIVRAQPFSLIGEIV 446
>Y477_CHLPN RecName: Full=Putative methylthiotransferase
CPn_0477/CP_0277/CPj0477/CpB0496; EC=2.-.-.-;
Length = 421
Score = 229 bits (583), Expect = 4e-62
Identities = 138/429 (32%), Positives = 223/429 (51%), Gaps = 25/429 (5%)
Query: 8 LGCRVNTYESEAMTEKFVREGY-EVVDFNEMADVYVVNTCSVTNMGDKKSRQIIGRARRQ 66
LGCRVN YE +A ++ GY EV+D AD+ ++NTC+VT + R + + RQ
Sbjct: 15 LGCRVNQYEVQAYRDQLTILGYQEVLDSEIPADLCIINTCAVTASAESSGRHAVRQLCRQ 74
Query: 67 NPEAIIAVVGCYAQIAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVKVGAVLK 126
NP A I V GC + + ++ ++ ++ K ++ +
Sbjct: 75 NPTAHIVVTGCLGESDKEFFASLDRQCTLVSNKEKSRLIEKIFSY--------------- 119
Query: 127 NKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDEIRSLAEH 186
+ F + KI + K+RAF+K+QDGCN FC+YC+IPY RG S+ +K+L EI + +
Sbjct: 120 DTTFPEFKIHSFEGKSRAFIKVQDGCNSFCSYCIIPYLRGRSVSRPAEKILAEIAGVVDQ 179
Query: 187 GFKEIILSGIHTASYGVDLDEKVTLVDLLEEIEKIDGIERVRIGSIDPTFFTEDVVRRIL 246
G++E++++GI+ Y D + +L L+E++++I G TED+ R I
Sbjct: 180 GYREVVIAGINVGDY---CDGERSLASLIEQVDQIPGXXXXXXXXXXXXXXTEDLHRAIT 236
Query: 247 ALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITTDVIVGFPGX 306
+ + CP HL LQSG ++ LKRMNR+Y+ ++ D V R + TTDVIVGFPG
Sbjct: 237 SSRHTCPSSHLVLQSGSNSILKRMNRKYSRGDFLDCVEKFRASDPRYAFTTDVIVGFPGE 296
Query: 307 XXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSKKLIEL-NRIN 365
++ + K H F +S R+ T+A +NQ+ ER K L E+ R+
Sbjct: 297 SDQDFEDTLRIIEDVGFIKVHSFPFSARRRTKAYTFDNQIPNQVIYERKKYLAEVAKRVG 356
Query: 366 EKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVS-GKILNVKINSFE 424
+K K +GE +VL E ++ V TG++ + KV+ V+ +++V+++ E
Sbjct: 357 QKEMM-KRLGETTEVLVE---KVTGQVATGHSPYFEKVSFPVVGTVAINTLVSVRLDRVE 412
Query: 425 GEVAEGEIV 433
E GEIV
Sbjct: 413 EEGLIGEIV 421
>MIAB_AKKM8 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 458
Score = 228 bits (580), Expect = 1e-61
Identities = 139/446 (31%), Positives = 232/446 (52%), Gaps = 24/446 (5%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKS---RQ 58
K+ T GC++N +SE + FV++GY + D + ADV + N+CS+ ++K+
Sbjct: 3 KLYIKTYGCQMNERDSEQVARMFVQKGYTMTDREDEADVILFNSCSIREQAEQKALGKMG 62
Query: 59 IIGRARRQNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVN---KARDE 114
++ + +R P + ++GC AQ E+ + +D+V+G++ + +V+ +AR E
Sbjct: 63 LLAKQQRHRPHVVYGMMGCMAQSKKEELFKELPRLDLVVGTQKYHRVFEHVDGILRARQE 122
Query: 115 GKQQVKVGAVLKNKVFEDLKIEEYNNKTR----------AFLKIQDGCNRFCAYCLIPYT 164
+ A V + + + N+ R A++ I GC CAYC++PYT
Sbjct: 123 RRMDELQTAFSGTHVCDVAEEADSQNRIRDHLNPGVRSTAYVSIMQGCEMKCAYCIVPYT 182
Query: 165 RGSVCSKDPKKVLDEIRSLAEHGFKEIILSGIHTASYGVDLDE---KVTLVDLLEEIEKI 221
RG+ S+ + V+DE++ LA+ G KE+ L G YG ++ K V LLE + ++
Sbjct: 183 RGAERSRPIRDVVDEVKMLADAGVKEVTLLGQIVNRYGRQMETAGGKGGFVQLLEAVHEV 242
Query: 222 DGIERVRIGSIDPTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYED 281
+GI R+R S P F +D+V+ L KLC H H +QSG D LK M R Y + Y D
Sbjct: 243 EGIRRIRFVSPHPIGFRQDLVQAFTYLPKLCSHIHFPMQSGSDRILKMMRRPYRNETYLD 302
Query: 282 IVNLLRDKIEDVSITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAEN 341
+ + ++ D+SITTD+IVGFPG ++++ IF+YSPR+GT A
Sbjct: 303 LCSRMKQARPDLSITTDIIVGFPGETEEDYLLTRQAVEQVQFDNAFIFRYSPRRGTPAAV 362
Query: 342 MENQVDGNKKDERSKKLIELNRINEKAFAEK--YIGEVIDVLFEEEVELGSGVYTGYTRN 399
MENQ+ K+ R++ L+ + +NE A + +G V +VL E + + +G T
Sbjct: 363 MENQIPEEVKEARNQDLLAV--VNEIAIRKNRDLVGTVQEVLLEGPSKTNAARLSGRTSQ 420
Query: 400 YIKVNAKADCNVSGKILNVKINSFEG 425
V A +++G+IL ++I G
Sbjct: 421 NKPVMVDAAPDLAGEILPIRIEESTG 446
>MIAB_FINM2 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 461
Score = 227 bits (579), Expect = 1e-61
Identities = 134/436 (30%), Positives = 218/436 (50%), Gaps = 15/436 (3%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIG---RA 63
T GC++N ++SE + G+E D AD + NTC V + K +G
Sbjct: 31 THGCQMNEHDSEKIKTLLENMGFEKSDEKLDADFIIFNTCLVRENAEMKVYGQLGALKNL 90
Query: 64 RRQNPEAIIAVVGCYAQIAPTEVSGIEG---VDVVLGSRNKGEIVYYVNKARDEGKQQVK 120
+R+NP+ +IAV GC Q P E VD+V G N + Y +++ GK V
Sbjct: 91 KRENPDMLIAVCGCMMQTGPARDIIREKYPQVDIVFGVNNINSLPYLIDRHLSSGKLVVX 150
Query: 121 VGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDEI 180
N+ ++ I GCN FC YC++PY RG S+ + +L E+
Sbjct: 151 XXXXXXXXXXXXXXXX---NEYVGYVNIMTGCNNFCTYCIVPYARGREQSRSVESILSEV 207
Query: 181 RSLAEHGFKEIILSGIHTASYGVDLDEKVTLVDLLEEIEKIDGIERVRIGSIDPTFFTED 240
+ + + G+K+I L G + SYG L+ VT +LL ++ ++G+ER+R + P +++
Sbjct: 208 KRMVDQGYKDITLLGQNVNSYGKTLENPVTFTELLTKVNDVEGLERLRFLTSHPKDISDE 267
Query: 241 VVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITTDVI 300
++ + L K+C + HL Q+G ++ L+RM+RRYT ++ +TD+I
Sbjct: 268 LIEAMGKLDKVCENIHLPFQAGSNSVLERMHRRYTXXXXXXXXXXXXXXXXGITFSTDII 327
Query: 301 VGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSKKLIE 360
VGFPG ++++ + FKY+ R GT+A+ E+QVD + K +R ++L++
Sbjct: 328 VGFPGETEEDFQDTLDVVRKVGYEQAFTFKYNRRPGTKADLFEDQVDEDVKQDRLERLLD 387
Query: 361 LNRINEKAFAEK---YIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSGKILN 417
+ F EK Y+G + +VL E E + V TG TR + VN K D + GK++N
Sbjct: 388 ---VAYPIFYEKNKSYLGTIQEVLIEGESKNNPEVMTGRTRTFKLVNVKCDKSYIGKLVN 444
Query: 418 VKINSFEGEVAEGEIV 433
KI F GE+V
Sbjct: 445 TKIVDFNSFALTGEMV 460
>MIAB_BACCN RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 509
Score = 226 bits (576), Expect = 3e-61
Identities = 142/439 (32%), Positives = 230/439 (52%), Gaps = 10/439 (2%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIG 61
K T GC++N +++E M F GYE E ADV ++NTC++ + K +G
Sbjct: 67 KFYIRTYGCQMNEHDTEVMAGIFTTLGYEPTFTTEDADVILLNTCAIRENAENKVFGELG 126
Query: 62 RAR---RQNPEAIIAVVGCYAQ---IAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEG 115
+ ++NP+ +I V GC +Q + + + VD+V G+ N + Y + A
Sbjct: 127 HLKPLKQKNPDLLIGVCGCMSQEESVVNKIMQKHQHVDMVFGTHNIHRLPYILKDAMFSK 186
Query: 116 KQQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKK 175
V+V + + V E+L + +A++ I GC++FC YC++PYTRG S+ P+
Sbjct: 187 ATVVEVWSK-EGDVIENLP-KVRRGDIKAWVNIMYGCDKFCTYCIVPYTRGKERSRRPED 244
Query: 176 VLDEIRSLAEHGFKEIILSGIHTASYGVDLDE-KVTLVDLLEEIEKIDGIERVRIGSIDP 234
++ E+R LA +G+KEI L G + +YG D D+ + L DL++E+ KID I R+R + P
Sbjct: 245 IIKEVRHLAANGYKEITLLGQNVNAYGKDFDDLEYGLGDLMDELRKID-IARIRFTTSHP 303
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
F + ++ + L H HL +QSG LK M R+YT ++Y ++V ++ I +V
Sbjct: 304 RDFDDHLIEVLGKGGNLVEHIHLPVQSGSTDMLKIMARKYTREQYLELVRKIKKTIPNVV 363
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
+TTD+IVGFP + + F YSPR+GT A M++ + K ER
Sbjct: 364 LTTDIIVGFPNETDEQFEETLSLYREVEFDSAFTFIYSPREGTPAAKMKDNIPMEVKKER 423
Query: 355 SKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSGK 414
++L EL +KY G++++VL + E + V GYTR VN A +V G+
Sbjct: 424 LQRLNELVNEFSAKKNKKYEGQIVEVLVDGESKNNPDVLAGYTRTNKLVNFVAPKSVIGQ 483
Query: 415 ILNVKINSFEGEVAEGEIV 433
++ VKI + GE+V
Sbjct: 484 LVKVKITEAKTWSLNGELV 502
>MIAB_CARHZ RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 440
Score = 225 bits (574), Expect = 5e-61
Identities = 138/434 (31%), Positives = 226/434 (52%), Gaps = 10/434 (2%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIG---RA 63
T GC++N ++SE + G+ + AD+ ++NTCSV + K IG +
Sbjct: 10 THGCQMNVHDSETIAGILESMGFVPSPEEKTADLIIINTCSVRETAENKVFTKIGELKKL 69
Query: 64 RRQNPEAIIAVVGCYAQ---IAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVK 120
+R+NP+ +I V GC Q +A + +D + G+ N E+ + + ++ ++ ++
Sbjct: 70 KRENPDLVIGVGGCIPQQEKVAKRLLERFPHLDFIFGTHNLPELPKILERVFEKHERVLE 129
Query: 121 VGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDEI 180
V + ++ E + ++ RA++ I GCN FC YC++PY RG S+ + +L EI
Sbjct: 130 VWQS-EGQIVEGIPVKREPG-VRAWVTIMYGCNNFCTYCIVPYVRGRERSRKKEDILQEI 187
Query: 181 RSLAEHGFKEIILSGIHTASYGVDLDEKVTLVDLLEEIEKIDGIERVRIGSIDPTFFTED 240
R L G++E+ L G + SYG DL K +LL +IEKIDG+ RVR + P T+D
Sbjct: 188 RQLVAEGYREVTLLGQNVNSYGKDLKGKPMFAELLADIEKIDGLWRVRFTTSHPRDLTDD 247
Query: 241 VVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITTDVI 300
V+ + + +K+C H HL +Q+G + LK M+R YT + Y ++V +R KI VS TTD+I
Sbjct: 248 VIEVMASSRKICEHLHLPVQAGSNKILKAMHRGYTREYYLNLVEKIRAKIPKVSFTTDII 307
Query: 301 VGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSKKLIE 360
VGFPG ++++ F Y+ R GT A M++QV K R ++LIE
Sbjct: 308 VGFPGETEEDFEQTLDLVRKVRYDSAFTFVYNKRTGTPAAEMKDQVPEEVKSRRIQELIE 367
Query: 361 LNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSGKILNVKI 420
L + G + ++L E + + G TR V + ++ GK++ VKI
Sbjct: 368 LQNGISLELNKNEEGNIHEILVEGKSKTDETKLAGRTRTNKLVVFNGNEDLVGKLVKVKI 427
Query: 421 NSFEGEVAEGEIVL 434
EG++ E VL
Sbjct: 428 T--EGKLFHLEGVL 439
>MIAB_BACWK RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 509
Score = 224 bits (572), Expect = 8e-61
Identities = 144/441 (32%), Positives = 233/441 (52%), Gaps = 14/441 (3%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIG 61
K T GC++N +++E M F GYE E ADV ++NTC++ + K +G
Sbjct: 67 KFYIRTYGCQMNEHDTEVMAGIFTTLGYEPTFSTEEADVILLNTCAIRENAENKVFGELG 126
Query: 62 RAR---RQNPEAIIAVVGCYAQ---IAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEG 115
+ R+NP+ II V GC +Q + + + VD+V G+ N + Y + A
Sbjct: 127 HLKSLKRRNPDLIIGVCGCMSQEESVVNKIMQKNQHVDMVFGTHNIHRLPYILKDAMFSK 186
Query: 116 KQQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKK 175
+ V+V + + V E+L + +A++ I GC++FC YC++PYTRG S+ P+
Sbjct: 187 ETVVEVWSK-EGDVIENLP-KVRRGDIKAWVNIMYGCDKFCTYCIVPYTRGKERSRRPED 244
Query: 176 VLDEIRSLAEHGFKEIILSGIHTASYGVDLDE-KVTLVDLLEEIEKIDGIERVRIGSIDP 234
++ EIR LA +G+KEI L G + +YG D ++ + L DL++E+ KID I R+R + P
Sbjct: 245 IIKEIRHLAANGYKEITLLGQNVNAYGKDFEDIQYGLGDLMDELRKID-IARIRFTTSHP 303
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
F + ++ + L H HL +QSG LK M R+Y+ + Y ++V +++ I +
Sbjct: 304 RDFDDHLIDVLGKGGNLVEHIHLPVQSGSTDMLKIMARKYSREHYLELVRKIKETIPNAV 363
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
+TTD+IVGFP + + F YSPR+GT A M++ V K ER
Sbjct: 364 LTTDIIVGFPNETDEQFEETMSLYREVGFDTAFTFIYSPREGTPAAKMQDNVPMEVKKER 423
Query: 355 SKKLIELNRINEKAF--AEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVS 412
++L L +NE ++YIG++++VL E E + V GYTR VN A ++
Sbjct: 424 LQRLNTL--VNEYGVNKNKRYIGQIVEVLVEGESKNNPEVLAGYTRTNKLVNFVASKSLI 481
Query: 413 GKILNVKINSFEGEVAEGEIV 433
G+++ VKI + GE+V
Sbjct: 482 GQLVKVKITEAKTWSLNGELV 502
>MIAB_DESAP RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 443
Score = 224 bits (570), Expect = 1e-60
Identities = 125/363 (34%), Positives = 198/363 (54%), Gaps = 8/363 (2%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIG---RA 63
T GC++N ++SE MT GYE AD+ ++NTC V + + ++G R
Sbjct: 12 TFGCQMNEFDSELMTGLLEGMGYEPAKSLREADLVLINTCCVRESAENRVWGLLGSLKRY 71
Query: 64 RRQNPEAIIAVVGCYAQIAPTEVSGIEG---VDVVLGSRNKGEIVYYVNKARDEGKQQVK 120
+R PE I+AV GC Q T I VD+VLG+ N+ E+ + + R G++ V
Sbjct: 72 KRDKPELIVAVSGCLPQQEGTAEEIIRRFPVVDLVLGTHNRHELPGLIEEVR-AGRRPVL 130
Query: 121 VGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDEI 180
+ V E L + + RA++ + GCN FC YC++PY RG CS+ P V+DE+
Sbjct: 131 GVRQPDSAVPEGLPVRRKSG-LRAWVPVIHGCNNFCTYCVVPYVRGRECSRRPDAVVDEV 189
Query: 181 RSLAEHGFKEIILSGIHTASYGVDLDEKVTLVDLLEEIEKIDGIERVRIGSIDPTFFTED 240
LA G++E+ L G + SYG DL E + LL ++ ++G+ R+R + P FT+
Sbjct: 190 CGLAAAGYREVTLLGQNVNSYGRDLGEGIDFAALLARLDGVEGLWRIRFTTSHPRDFTDR 249
Query: 241 VVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITTDVI 300
++ + K+C H HL Q+G + L+RMNR YT ++Y D+V +R + DVS+TTD++
Sbjct: 250 LIEVVARAAKVCEHIHLPAQAGSNRVLQRMNRGYTREDYLDLVARIRAAVPDVSLTTDLM 309
Query: 301 VGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSKKLIE 360
VGFPG ++R+ + F Y+PR+GT A +QV + K R ++LI+
Sbjct: 310 VGFPGETEEDFADTLDLVRRVGYDQAFTFVYNPRRGTPAAGWPDQVPEDVKSRRIQELIQ 369
Query: 361 LNR 363
+ +
Sbjct: 370 VQK 372
>MIAB_STAAW RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 514
Score = 223 bits (569), Expect = 2e-60
Identities = 142/435 (32%), Positives = 226/435 (51%), Gaps = 12/435 (2%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIGRAR-- 64
T GC++N +++E + GY+ ADV ++NTC++ + K IG +
Sbjct: 74 TYGCQMNAHDTEVIAGILEALGYQATTDINTADVILINTCAIRENAENKVFSEIGNLKHL 133
Query: 65 -RQNPEAIIAVVGCYAQ---IAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVK 120
++ P+ +I V GC +Q + + + VD++ G+ N + + +A V+
Sbjct: 134 KKERPDILIGVCGCMSQEESVVNKILKSYQNVDMIFGTHNIHHLPEILEEAYLSKAMVVE 193
Query: 121 VGAVLKNKVFEDL-KIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDE 179
V + + V E+L K+ E N K A++ I GC++FC YC++P+TRG S+ P+ ++DE
Sbjct: 194 VWSK-EGDVIENLPKVREGNIK--AWVNIMYGCDKFCTYCIVPFTRGKERSRRPEDIIDE 250
Query: 180 IRSLAEHGFKEIILSGIHTASYGVDL-DEKVTLVDLLEEIEKIDGIERVRIGSIDPTFFT 238
+R LA G+KEI L G + SYG DL D + L DLL+ I KI I RVR + P FT
Sbjct: 251 VRELAREGYKEITLLGQNVNSYGKDLQDIEYDLGDLLQAISKI-AIPRVRFTTSHPWDFT 309
Query: 239 EDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITTD 298
+ ++ I + PH HL +QSG +A LK M R+YT + Y D+V ++D+I +V++TTD
Sbjct: 310 DHMIDVISEGGNIVPHIHLPVQSGNNAVLKIMGRKYTRESYLDLVKRIKDRIPNVALTTD 369
Query: 299 VIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSKKL 358
+IVG+P + + + YS R GT A M++ V N K ER ++L
Sbjct: 370 IIVGYPNESEEQFEETLTLYDEVGFEHAYTYLYSQRDGTPAAKMKDNVPLNVKKERLQRL 429
Query: 359 IELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSGKILNV 418
+ + KY G+ + VL E + V GYT VN KA + GK++ V
Sbjct: 430 NKKVGHYSQIAMSKYEGQTVTVLCEGSSKKDDQVLAGYTDKNKLVNFKAPKEMIGKLVEV 489
Query: 419 KINSFEGEVAEGEIV 433
+I+ + G V
Sbjct: 490 RIDEAKQYSLNGSFV 504
>MIAB_STAAS RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 514
Score = 223 bits (569), Expect = 2e-60
Identities = 142/435 (32%), Positives = 226/435 (51%), Gaps = 12/435 (2%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIGRAR-- 64
T GC++N +++E + GY+ ADV ++NTC++ + K IG +
Sbjct: 74 TYGCQMNAHDTEVIAGILEALGYQATTDINTADVILINTCAIRENAENKVFSEIGNLKHL 133
Query: 65 -RQNPEAIIAVVGCYAQ---IAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVK 120
++ P+ +I V GC +Q + + + VD++ G+ N + + +A V+
Sbjct: 134 KKERPDILIGVCGCMSQEESVVNKILKSYQNVDMIFGTHNIHHLPEILEEAYLSKAMVVE 193
Query: 121 VGAVLKNKVFEDL-KIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDE 179
V + + V E+L K+ E N K A++ I GC++FC YC++P+TRG S+ P+ ++DE
Sbjct: 194 VWSK-EGDVIENLPKVREGNIK--AWVNIMYGCDKFCTYCIVPFTRGKERSRRPEDIIDE 250
Query: 180 IRSLAEHGFKEIILSGIHTASYGVDL-DEKVTLVDLLEEIEKIDGIERVRIGSIDPTFFT 238
+R LA G+KEI L G + SYG DL D + L DLL+ I KI I RVR + P FT
Sbjct: 251 VRELAREGYKEITLLGQNVNSYGKDLQDIEYDLGDLLQAISKI-AIPRVRFTTSHPWDFT 309
Query: 239 EDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITTD 298
+ ++ I + PH HL +QSG +A LK M R+YT + Y D+V ++D+I +V++TTD
Sbjct: 310 DHMIDVISEGGNIVPHIHLPVQSGNNAVLKIMGRKYTRESYLDLVKRIKDRIPNVALTTD 369
Query: 299 VIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSKKL 358
+IVG+P + + + YS R GT A M++ V N K ER ++L
Sbjct: 370 IIVGYPNESEEQFEETLTLYDEVGFEHAYTYLYSQRDGTPAAKMKDNVPLNVKKERLQRL 429
Query: 359 IELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSGKILNV 418
+ + KY G+ + VL E + V GYT VN KA + GK++ V
Sbjct: 430 NKKVGHYSQIAMSKYEGQTVTVLCEGSSKKDDQVLAGYTDKNKLVNFKAPKEMIGKLVEV 489
Query: 419 KINSFEGEVAEGEIV 433
+I+ + G V
Sbjct: 490 RIDEAKQYSLNGSFV 504
>MIAB_STAAN RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 514
Score = 223 bits (569), Expect = 2e-60
Identities = 142/435 (32%), Positives = 226/435 (51%), Gaps = 12/435 (2%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIGRAR-- 64
T GC++N +++E + GY+ ADV ++NTC++ + K IG +
Sbjct: 74 TYGCQMNAHDTEVIAGILEALGYQATTDINTADVILINTCAIRENAENKVFSEIGNLKHL 133
Query: 65 -RQNPEAIIAVVGCYAQ---IAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVK 120
++ P+ +I V GC +Q + + + VD++ G+ N + + +A V+
Sbjct: 134 KKERPDILIGVCGCMSQEESVVNKILKSYQNVDMIFGTHNIHHLPEILEEAYLSKAMVVE 193
Query: 121 VGAVLKNKVFEDL-KIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDE 179
V + + V E+L K+ E N K A++ I GC++FC YC++P+TRG S+ P+ ++DE
Sbjct: 194 VWSK-EGDVIENLPKVREGNIK--AWVNIMYGCDKFCTYCIVPFTRGKERSRRPEDIIDE 250
Query: 180 IRSLAEHGFKEIILSGIHTASYGVDL-DEKVTLVDLLEEIEKIDGIERVRIGSIDPTFFT 238
+R LA G+KEI L G + SYG DL D + L DLL+ I KI I RVR + P FT
Sbjct: 251 VRELAREGYKEITLLGQNVNSYGKDLQDIEYDLGDLLQAISKI-AIPRVRFTTSHPWDFT 309
Query: 239 EDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITTD 298
+ ++ I + PH HL +QSG +A LK M R+YT + Y D+V ++D+I +V++TTD
Sbjct: 310 DHMIDVISEGGNIVPHIHLPVQSGNNAVLKIMGRKYTRESYLDLVKRIKDRIPNVALTTD 369
Query: 299 VIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSKKL 358
+IVG+P + + + YS R GT A M++ V N K ER ++L
Sbjct: 370 IIVGYPNESEEQFEETLTLYDEVGFEHAYTYLYSQRDGTPAAKMKDNVPLNVKKERLQRL 429
Query: 359 IELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSGKILNV 418
+ + KY G+ + VL E + V GYT VN KA + GK++ V
Sbjct: 430 NKKVGHYSQIAMSKYEGQTVTVLCEGSSKKDDQVLAGYTDKNKLVNFKAPKEMIGKLVEV 489
Query: 419 KINSFEGEVAEGEIV 433
+I+ + G V
Sbjct: 490 RIDEAKQYSLNGSFV 504
>MIAB_STAAM RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 514
Score = 223 bits (569), Expect = 2e-60
Identities = 142/435 (32%), Positives = 226/435 (51%), Gaps = 12/435 (2%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIGRAR-- 64
T GC++N +++E + GY+ ADV ++NTC++ + K IG +
Sbjct: 74 TYGCQMNAHDTEVIAGILEALGYQATTDINTADVILINTCAIRENAENKVFSEIGNLKHL 133
Query: 65 -RQNPEAIIAVVGCYAQ---IAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVK 120
++ P+ +I V GC +Q + + + VD++ G+ N + + +A V+
Sbjct: 134 KKERPDILIGVCGCMSQEESVVNKILKSYQNVDMIFGTHNIHHLPEILEEAYLSKAMVVE 193
Query: 121 VGAVLKNKVFEDL-KIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDE 179
V + + V E+L K+ E N K A++ I GC++FC YC++P+TRG S+ P+ ++DE
Sbjct: 194 VWSK-EGDVIENLPKVREGNIK--AWVNIMYGCDKFCTYCIVPFTRGKERSRRPEDIIDE 250
Query: 180 IRSLAEHGFKEIILSGIHTASYGVDL-DEKVTLVDLLEEIEKIDGIERVRIGSIDPTFFT 238
+R LA G+KEI L G + SYG DL D + L DLL+ I KI I RVR + P FT
Sbjct: 251 VRELAREGYKEITLLGQNVNSYGKDLQDIEYDLGDLLQAISKI-AIPRVRFTTSHPWDFT 309
Query: 239 EDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITTD 298
+ ++ I + PH HL +QSG +A LK M R+YT + Y D+V ++D+I +V++TTD
Sbjct: 310 DHMIDVISEGGNIVPHIHLPVQSGNNAVLKIMGRKYTRESYLDLVKRIKDRIPNVALTTD 369
Query: 299 VIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSKKL 358
+IVG+P + + + YS R GT A M++ V N K ER ++L
Sbjct: 370 IIVGYPNESEEQFEETLTLYDEVGFEHAYTYLYSQRDGTPAAKMKDNVPLNVKKERLQRL 429
Query: 359 IELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSGKILNV 418
+ + KY G+ + VL E + V GYT VN KA + GK++ V
Sbjct: 430 NKKVGHYSQIAMSKYEGQTVTVLCEGSSKKDDQVLAGYTDKNKLVNFKAPKEMIGKLVEV 489
Query: 419 KINSFEGEVAEGEIV 433
+I+ + G V
Sbjct: 490 RIDEAKQYSLNGSFV 504
>MIAB_STAA9 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 514
Score = 223 bits (569), Expect = 2e-60
Identities = 142/435 (32%), Positives = 226/435 (51%), Gaps = 12/435 (2%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIGRAR-- 64
T GC++N +++E + GY+ ADV ++NTC++ + K IG +
Sbjct: 74 TYGCQMNAHDTEVIAGILEALGYQATTDINTADVILINTCAIRENAENKVFSEIGNLKHL 133
Query: 65 -RQNPEAIIAVVGCYAQ---IAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVK 120
++ P+ +I V GC +Q + + + VD++ G+ N + + +A V+
Sbjct: 134 KKERPDILIGVCGCMSQEESVVNKILKSYQNVDMIFGTHNIHHLPEILEEAYLSKAMVVE 193
Query: 121 VGAVLKNKVFEDL-KIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDE 179
V + + V E+L K+ E N K A++ I GC++FC YC++P+TRG S+ P+ ++DE
Sbjct: 194 VWSK-EGDVIENLPKVREGNIK--AWVNIMYGCDKFCTYCIVPFTRGKERSRRPEDIIDE 250
Query: 180 IRSLAEHGFKEIILSGIHTASYGVDL-DEKVTLVDLLEEIEKIDGIERVRIGSIDPTFFT 238
+R LA G+KEI L G + SYG DL D + L DLL+ I KI I RVR + P FT
Sbjct: 251 VRELAREGYKEITLLGQNVNSYGKDLQDIEYDLGDLLQAISKI-AIPRVRFTTSHPWDFT 309
Query: 239 EDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITTD 298
+ ++ I + PH HL +QSG +A LK M R+YT + Y D+V ++D+I +V++TTD
Sbjct: 310 DHMIDVISEGGNIVPHIHLPVQSGNNAVLKIMGRKYTRESYLDLVKRIKDRIPNVALTTD 369
Query: 299 VIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSKKL 358
+IVG+P + + + YS R GT A M++ V N K ER ++L
Sbjct: 370 IIVGYPNESEEQFEETLTLYDEVGFEHAYTYLYSQRDGTPAAKMKDNVPLNVKKERLQRL 429
Query: 359 IELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSGKILNV 418
+ + KY G+ + VL E + V GYT VN KA + GK++ V
Sbjct: 430 NKKVGHYSQIAMSKYEGQTVTVLCEGSSKKDDQVLAGYTDKNKLVNFKAPKEMIGKLVEV 489
Query: 419 KINSFEGEVAEGEIV 433
+I+ + G V
Sbjct: 490 RIDEAKQYSLNGSFV 504
>MIAB_STAA2 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 514
Score = 223 bits (569), Expect = 2e-60
Identities = 142/435 (32%), Positives = 226/435 (51%), Gaps = 12/435 (2%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIGRAR-- 64
T GC++N +++E + GY+ ADV ++NTC++ + K IG +
Sbjct: 74 TYGCQMNAHDTEVIAGILEALGYQATTDINTADVILINTCAIRENAENKVFSEIGNLKHL 133
Query: 65 -RQNPEAIIAVVGCYAQ---IAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVK 120
++ P+ +I V GC +Q + + + VD++ G+ N + + +A V+
Sbjct: 134 KKERPDILIGVCGCMSQEESVVNKILKSYQNVDMIFGTHNIHHLPEILEEAYLSKAMVVE 193
Query: 121 VGAVLKNKVFEDL-KIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDE 179
V + + V E+L K+ E N K A++ I GC++FC YC++P+TRG S+ P+ ++DE
Sbjct: 194 VWSK-EGDVIENLPKVREGNIK--AWVNIMYGCDKFCTYCIVPFTRGKERSRRPEDIIDE 250
Query: 180 IRSLAEHGFKEIILSGIHTASYGVDL-DEKVTLVDLLEEIEKIDGIERVRIGSIDPTFFT 238
+R LA G+KEI L G + SYG DL D + L DLL+ I KI I RVR + P FT
Sbjct: 251 VRELAREGYKEITLLGQNVNSYGKDLQDIEYDLGDLLQAISKI-AIPRVRFTTSHPWDFT 309
Query: 239 EDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITTD 298
+ ++ I + PH HL +QSG +A LK M R+YT + Y D+V ++D+I +V++TTD
Sbjct: 310 DHMIDVISEGGNIVPHIHLPVQSGNNAVLKIMGRKYTRESYLDLVKRIKDRIPNVALTTD 369
Query: 299 VIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSKKL 358
+IVG+P + + + YS R GT A M++ V N K ER ++L
Sbjct: 370 IIVGYPNESEEQFEETLTLYDEVGFEHAYTYLYSQRDGTPAAKMKDNVPLNVKKERLQRL 429
Query: 359 IELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSGKILNV 418
+ + KY G+ + VL E + V GYT VN KA + GK++ V
Sbjct: 430 NKKVGHYSQIAMSKYEGQTVTVLCEGSSKKDDQVLAGYTDKNKLVNFKAPKEMIGKLVEV 489
Query: 419 KINSFEGEVAEGEIV 433
+I+ + G V
Sbjct: 490 RIDEAKQYSLNGSFV 504
>MIAB_STAA1 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 514
Score = 223 bits (569), Expect = 2e-60
Identities = 142/435 (32%), Positives = 226/435 (51%), Gaps = 12/435 (2%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIGRAR-- 64
T GC++N +++E + GY+ ADV ++NTC++ + K IG +
Sbjct: 74 TYGCQMNAHDTEVIAGILEALGYQATTDINTADVILINTCAIRENAENKVFSEIGNLKHL 133
Query: 65 -RQNPEAIIAVVGCYAQ---IAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVK 120
++ P+ +I V GC +Q + + + VD++ G+ N + + +A V+
Sbjct: 134 KKERPDILIGVCGCMSQEESVVNKILKSYQNVDMIFGTHNIHHLPEILEEAYLSKAMVVE 193
Query: 121 VGAVLKNKVFEDL-KIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDE 179
V + + V E+L K+ E N K A++ I GC++FC YC++P+TRG S+ P+ ++DE
Sbjct: 194 VWSK-EGDVIENLPKVREGNIK--AWVNIMYGCDKFCTYCIVPFTRGKERSRRPEDIIDE 250
Query: 180 IRSLAEHGFKEIILSGIHTASYGVDL-DEKVTLVDLLEEIEKIDGIERVRIGSIDPTFFT 238
+R LA G+KEI L G + SYG DL D + L DLL+ I KI I RVR + P FT
Sbjct: 251 VRELAREGYKEITLLGQNVNSYGKDLQDIEYDLGDLLQAISKI-AIPRVRFTTSHPWDFT 309
Query: 239 EDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITTD 298
+ ++ I + PH HL +QSG +A LK M R+YT + Y D+V ++D+I +V++TTD
Sbjct: 310 DHMIDVISEGGNIVPHIHLPVQSGNNAVLKIMGRKYTRESYLDLVKRIKDRIPNVALTTD 369
Query: 299 VIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSKKL 358
+IVG+P + + + YS R GT A M++ V N K ER ++L
Sbjct: 370 IIVGYPNESEEQFEETLTLYDEVGFEHAYTYLYSQRDGTPAAKMKDNVPLNVKKERLQRL 429
Query: 359 IELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSGKILNV 418
+ + KY G+ + VL E + V GYT VN KA + GK++ V
Sbjct: 430 NKKVGHYSQIAMSKYEGQTVTVLCEGSSKKDDQVLAGYTDKNKLVNFKAPKEMIGKLVEV 489
Query: 419 KINSFEGEVAEGEIV 433
+I+ + G V
Sbjct: 490 RIDEAKQYSLNGSFV 504
>MIAB_BACC4 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 509
Score = 223 bits (569), Expect = 2e-60
Identities = 142/441 (32%), Positives = 233/441 (52%), Gaps = 14/441 (3%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIG 61
K T GC++N +++E M F GYE E ADV ++NTC++ + K +G
Sbjct: 67 KFYIRTYGCQMNEHDTEVMAGIFTALGYEPTFSTEDADVVLLNTCAIRENAENKVFGELG 126
Query: 62 RAR---RQNPEAIIAVVGCYAQ---IAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEG 115
+ R+NP+ +I V GC +Q + + + VD+V G+ N + Y + A
Sbjct: 127 HLKSLKRRNPDLLIGVCGCMSQEESVVNKIMQKNQHVDMVFGTHNIHRLPYILKDAMFSK 186
Query: 116 KQQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKK 175
+ V+V + + V E+L + +A++ I GC++FC YC++PYTRG S+ P+
Sbjct: 187 ETVVEVWSK-EGDVIENLP-KVRRGDIKAWVNIMYGCDKFCTYCIVPYTRGKERSRRPED 244
Query: 176 VLDEIRSLAEHGFKEIILSGIHTASYGVDLDE-KVTLVDLLEEIEKIDGIERVRIGSIDP 234
++ EIR LA +G+KEI L G + +YG D ++ + L DL++E+ K+D I R+R + P
Sbjct: 245 IIQEIRHLAANGYKEITLLGQNVNAYGKDFEDIEYGLGDLMDELRKVD-IARIRFTTSHP 303
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
F + ++ + L H HL +QSG LK M R+Y+ + Y ++V +++ I DV
Sbjct: 304 RDFDDHLIEVLGKGGNLVEHIHLPVQSGSTDMLKIMARKYSREHYLELVRKIKEAIPDVV 363
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
+TTD+IVGFP + + F YSPR+GT A M++ V K ER
Sbjct: 364 LTTDIIVGFPNETDEQFEETMSLYREVGFDTAFTFIYSPREGTPAAKMKDNVPMEVKKER 423
Query: 355 SKKLIELNRINEKAFAE--KYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVS 412
++L L +N A + +Y G++++VL + E + V GYTR VN A ++
Sbjct: 424 LQRLNTL--VNTLAIEKNSRYKGQIVEVLVDGESKNNPEVLAGYTRTNKLVNFVASKSLI 481
Query: 413 GKILNVKINSFEGEVAEGEIV 433
G+++ VKI + GE+V
Sbjct: 482 GQLVKVKITEAKTWSLNGELV 502
>MIAB_STAS1 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 513
Score = 223 bits (568), Expect = 2e-60
Identities = 141/423 (33%), Positives = 225/423 (53%), Gaps = 12/423 (2%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIGRAR-- 64
T GC++N +++E M GY + ADV ++NTC++ + K IG +
Sbjct: 73 TYGCQMNAHDTEVMAGILGALGYTPTEDINHADVILINTCAIRENAENKVFSEIGNLKHL 132
Query: 65 -RQNPEAIIAVVGCYAQ---IAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVK 120
++ PE +I V GC +Q + + + VD++ G+ N + + +A V+
Sbjct: 133 KKEKPETVIGVCGCMSQEESVVNKILKSYQNVDMIFGTHNIHRLPEILEEAYLSKAMVVE 192
Query: 121 VGAVLKNKVFEDL-KIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDE 179
V + + V E+L K+ E N K A++ I GC++FC YC++P+TRG S+ P+ ++DE
Sbjct: 193 VWSK-EGDVIENLPKVREGNIK--AWVNIMYGCDKFCTYCIVPFTRGKERSRRPEDIIDE 249
Query: 180 IRSLAEHGFKEIILSGIHTASYGVDLDEKVT-LVDLLEEIEKIDGIERVRIGSIDPTFFT 238
+R LA G+KEI L G + +YG D+D L DLLE+I KID I RVR + P FT
Sbjct: 250 VRDLARQGYKEITLLGQNVNAYGKDIDGLAYGLGDLLEDISKID-IPRVRFTTSHPWDFT 308
Query: 239 EDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITTD 298
+ ++ I + PH HL +QSG +A LK M R+YT + Y D+VN ++ I +V++TTD
Sbjct: 309 DRMIEVIANGGNIVPHVHLPVQSGNNAVLKIMGRKYTRESYLDLVNRIKTHIPNVALTTD 368
Query: 299 VIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSKKL 358
+IVG+P + + + YS R GT A M + V + K +R ++L
Sbjct: 369 IIVGYPNETDEQFEETLTLYDEVEFEHAYTYIYSQRDGTPAAKMNDNVPLDVKKDRLQQL 428
Query: 359 IELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSGKILNV 418
+ + ++Y G+ + VL E + V +GYT VN KA ++ GKI+NV
Sbjct: 429 NKKVACYSERAMQQYEGQTVQVLCEGVSKKDDTVLSGYTSKNKLVNFKAPKSMIGKIVNV 488
Query: 419 KIN 421
I+
Sbjct: 489 YID 491
>MIAB_STAAT RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 514
Score = 223 bits (568), Expect = 2e-60
Identities = 141/435 (32%), Positives = 226/435 (51%), Gaps = 12/435 (2%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIGRAR-- 64
T GC++N +++E + GY+ ADV ++NTC++ + K IG +
Sbjct: 74 TYGCQMNAHDTEVIAGILEALGYQATTDINTADVILINTCAIRENAENKVFSEIGNLKHL 133
Query: 65 -RQNPEAIIAVVGCYAQ---IAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVK 120
++ P+ +I V GC +Q + + + VD++ G+ N + + +A V+
Sbjct: 134 KKERPDILIGVCGCMSQEESVVNKILKSYQNVDMIFGTHNIHHLPEILEEAYLSKAMVVE 193
Query: 121 VGAVLKNKVFEDL-KIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDE 179
V + + V E+L K+ E N K A++ I GC++FC YC++P+TRG S+ P+ ++DE
Sbjct: 194 VWSK-EGDVIENLPKVREGNIK--AWVNIMYGCDKFCTYCIVPFTRGKERSRRPEDIIDE 250
Query: 180 IRSLAEHGFKEIILSGIHTASYGVDL-DEKVTLVDLLEEIEKIDGIERVRIGSIDPTFFT 238
+R LA G+KEI L G + SYG DL D + L DLL+ I KI I RVR + P FT
Sbjct: 251 VRELAREGYKEITLLGQNVNSYGKDLQDIEYDLGDLLQAISKI-AIPRVRFTTSHPWDFT 309
Query: 239 EDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITTD 298
+ ++ I + PH HL +QSG +A LK M R+YT + Y D+V ++D+I +V++TTD
Sbjct: 310 DHMIDVISEGGNIVPHIHLPVQSGNNAVLKIMGRKYTRESYLDLVKRIKDRIPNVALTTD 369
Query: 299 VIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSKKL 358
+IVG+P + + + YS R GT A M++ V N K ER ++L
Sbjct: 370 IIVGYPNESEEQFEETLTLYDEVGFEHAYTYLYSQRDGTPAAKMKDNVPLNVKKERLQRL 429
Query: 359 IELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSGKILNV 418
+ + KY G+ + VL E + V GYT VN KA + GK++ V
Sbjct: 430 NKKVGHYSQIAMSKYEGQTVTVLCEGSSKKDDQVLAGYTDKNKLVNFKAPKEMIGKLVEV 489
Query: 419 KINSFEGEVAEGEIV 433
+I+ + G +
Sbjct: 490 RIDEAKQYSLNGSFI 504
>MIAB_STAAE RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 514
Score = 223 bits (568), Expect = 2e-60
Identities = 141/435 (32%), Positives = 226/435 (51%), Gaps = 12/435 (2%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIGRAR-- 64
T GC++N +++E + GY+ ADV ++NTC++ + K IG +
Sbjct: 74 TYGCQMNAHDTEVIAGILEALGYQATTDINTADVILINTCAIRENAENKVFSEIGNLKHL 133
Query: 65 -RQNPEAIIAVVGCYAQ---IAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVK 120
++ P+ +I V GC +Q + + + VD++ G+ N + + +A V+
Sbjct: 134 KKERPDILIGVCGCMSQEESVVNKILKSYQNVDMIFGTHNIHHLPEILEEAYLSKAMVVE 193
Query: 121 VGAVLKNKVFEDL-KIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDE 179
V + + V E+L K+ E N K A++ I GC++FC YC++P+TRG S+ P+ ++DE
Sbjct: 194 VWSK-EGDVIENLPKVREGNIK--AWVNIMYGCDKFCTYCIVPFTRGKERSRRPEDIIDE 250
Query: 180 IRSLAEHGFKEIILSGIHTASYGVDL-DEKVTLVDLLEEIEKIDGIERVRIGSIDPTFFT 238
+R LA G+KEI L G + SYG DL D + L DLL+ I KI I RVR + P FT
Sbjct: 251 VRELAREGYKEITLLGQNVNSYGKDLQDIEYDLGDLLQAISKI-AIPRVRFTTSHPWDFT 309
Query: 239 EDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITTD 298
+ ++ I + PH HL +QSG +A LK M R+YT + Y D+V ++D+I +V++TTD
Sbjct: 310 DHMIDVISEGGNIVPHIHLPVQSGNNAVLKIMGRKYTRESYLDLVKRIKDRIPNVALTTD 369
Query: 299 VIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSKKL 358
+IVG+P + + + YS R GT A M++ V N K ER ++L
Sbjct: 370 IIVGYPNESEEQFEETLTLYDEVGFEHAYTYLYSQRDGTPAAKMKDNVPLNVKKERLQRL 429
Query: 359 IELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSGKILNV 418
+ + KY G+ + VL E + V GYT VN KA + GK++ V
Sbjct: 430 NKKVGHYSQIAMSKYEGQTVTVLCEGSSKKDDQVLAGYTDKNKLVNFKAPKEMIGKLVEV 489
Query: 419 KINSFEGEVAEGEIV 433
+I+ + G +
Sbjct: 490 RIDEAKQYSLNGSFI 504
>MIAB_STAAC RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 514
Score = 223 bits (568), Expect = 2e-60
Identities = 141/435 (32%), Positives = 226/435 (51%), Gaps = 12/435 (2%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIGRAR-- 64
T GC++N +++E + GY+ ADV ++NTC++ + K IG +
Sbjct: 74 TYGCQMNAHDTEVIAGILEALGYQATTDINTADVILINTCAIRENAENKVFSEIGNLKHL 133
Query: 65 -RQNPEAIIAVVGCYAQ---IAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVK 120
++ P+ +I V GC +Q + + + VD++ G+ N + + +A V+
Sbjct: 134 KKERPDILIGVCGCMSQEESVVNKILKSYQNVDMIFGTHNIHHLPEILEEAYLSKAMVVE 193
Query: 121 VGAVLKNKVFEDL-KIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDE 179
V + + V E+L K+ E N K A++ I GC++FC YC++P+TRG S+ P+ ++DE
Sbjct: 194 VWSK-EGDVIENLPKVREGNIK--AWVNIMYGCDKFCTYCIVPFTRGKERSRRPEDIIDE 250
Query: 180 IRSLAEHGFKEIILSGIHTASYGVDL-DEKVTLVDLLEEIEKIDGIERVRIGSIDPTFFT 238
+R LA G+KEI L G + SYG DL D + L DLL+ I KI I RVR + P FT
Sbjct: 251 VRELAREGYKEITLLGQNVNSYGKDLQDIEYDLGDLLQAISKI-AIPRVRFTTSHPWDFT 309
Query: 239 EDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITTD 298
+ ++ I + PH HL +QSG +A LK M R+YT + Y D+V ++D+I +V++TTD
Sbjct: 310 DHMIDVISEGGNIVPHIHLPVQSGNNAVLKIMGRKYTRESYLDLVKRIKDRIPNVALTTD 369
Query: 299 VIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSKKL 358
+IVG+P + + + YS R GT A M++ V N K ER ++L
Sbjct: 370 IIVGYPNESEEQFEETLTLYDEVGFEHAYTYLYSQRDGTPAAKMKDNVPLNVKKERLQRL 429
Query: 359 IELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSGKILNV 418
+ + KY G+ + VL E + V GYT VN KA + GK++ V
Sbjct: 430 NKKVGHYSQIAMSKYEGQTVTVLCEGSSKKDDQVLAGYTDKNKLVNFKAPKEMIGKLVEV 489
Query: 419 KINSFEGEVAEGEIV 433
+I+ + G +
Sbjct: 490 RIDEAKQYSLNGSFI 504
>MIAB_STAA8 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 514
Score = 223 bits (568), Expect = 2e-60
Identities = 141/435 (32%), Positives = 226/435 (51%), Gaps = 12/435 (2%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIGRAR-- 64
T GC++N +++E + GY+ ADV ++NTC++ + K IG +
Sbjct: 74 TYGCQMNAHDTEVIAGILEALGYQATTDINTADVILINTCAIRENAENKVFSEIGNLKHL 133
Query: 65 -RQNPEAIIAVVGCYAQ---IAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVK 120
++ P+ +I V GC +Q + + + VD++ G+ N + + +A V+
Sbjct: 134 KKERPDILIGVCGCMSQEESVVNKILKSYQNVDMIFGTHNIHHLPEILEEAYLSKAMVVE 193
Query: 121 VGAVLKNKVFEDL-KIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDE 179
V + + V E+L K+ E N K A++ I GC++FC YC++P+TRG S+ P+ ++DE
Sbjct: 194 VWSK-EGDVIENLPKVREGNIK--AWVNIMYGCDKFCTYCIVPFTRGKERSRRPEDIIDE 250
Query: 180 IRSLAEHGFKEIILSGIHTASYGVDL-DEKVTLVDLLEEIEKIDGIERVRIGSIDPTFFT 238
+R LA G+KEI L G + SYG DL D + L DLL+ I KI I RVR + P FT
Sbjct: 251 VRELAREGYKEITLLGQNVNSYGKDLQDIEYDLGDLLQAISKI-AIPRVRFTTSHPWDFT 309
Query: 239 EDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITTD 298
+ ++ I + PH HL +QSG +A LK M R+YT + Y D+V ++D+I +V++TTD
Sbjct: 310 DHMIDVISEGGNIVPHIHLPVQSGNNAVLKIMGRKYTRESYLDLVKRIKDRIPNVALTTD 369
Query: 299 VIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSKKL 358
+IVG+P + + + YS R GT A M++ V N K ER ++L
Sbjct: 370 IIVGYPNESEEQFEETLTLYDEVGFEHAYTYLYSQRDGTPAAKMKDNVPLNVKKERLQRL 429
Query: 359 IELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSGKILNV 418
+ + KY G+ + VL E + V GYT VN KA + GK++ V
Sbjct: 430 NKKVGHYSQIAMSKYEGQTVTVLCEGSSKKDDQVLAGYTDKNKLVNFKAPKEMIGKLVEV 489
Query: 419 KINSFEGEVAEGEIV 433
+I+ + G +
Sbjct: 490 RIDEAKQYSLNGSFI 504
>MIAB_STAA3 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 514
Score = 223 bits (568), Expect = 2e-60
Identities = 141/435 (32%), Positives = 226/435 (51%), Gaps = 12/435 (2%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIGRAR-- 64
T GC++N +++E + GY+ ADV ++NTC++ + K IG +
Sbjct: 74 TYGCQMNAHDTEVIAGILEALGYQATTDINTADVILINTCAIRENAENKVFSEIGNLKHL 133
Query: 65 -RQNPEAIIAVVGCYAQ---IAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVK 120
++ P+ +I V GC +Q + + + VD++ G+ N + + +A V+
Sbjct: 134 KKERPDILIGVCGCMSQEESVVNKILKSYQNVDMIFGTHNIHHLPEILEEAYLSKAMVVE 193
Query: 121 VGAVLKNKVFEDL-KIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDE 179
V + + V E+L K+ E N K A++ I GC++FC YC++P+TRG S+ P+ ++DE
Sbjct: 194 VWSK-EGDVIENLPKVREGNIK--AWVNIMYGCDKFCTYCIVPFTRGKERSRRPEDIIDE 250
Query: 180 IRSLAEHGFKEIILSGIHTASYGVDL-DEKVTLVDLLEEIEKIDGIERVRIGSIDPTFFT 238
+R LA G+KEI L G + SYG DL D + L DLL+ I KI I RVR + P FT
Sbjct: 251 VRELAREGYKEITLLGQNVNSYGKDLQDIEYDLGDLLQAISKI-AIPRVRFTTSHPWDFT 309
Query: 239 EDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITTD 298
+ ++ I + PH HL +QSG +A LK M R+YT + Y D+V ++D+I +V++TTD
Sbjct: 310 DHMIDVISEGGNIVPHIHLPVQSGNNAVLKIMGRKYTRESYLDLVKRIKDRIPNVALTTD 369
Query: 299 VIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSKKL 358
+IVG+P + + + YS R GT A M++ V N K ER ++L
Sbjct: 370 IIVGYPNESEEQFEETLTLYDEVGFEHAYTYLYSQRDGTPAAKMKDNVPLNVKKERLQRL 429
Query: 359 IELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSGKILNV 418
+ + KY G+ + VL E + V GYT VN KA + GK++ V
Sbjct: 430 NKKVGHYSQIAMSKYEGQTVTVLCEGSSKKDDQVLAGYTDKNKLVNFKAPKEMIGKLVEV 489
Query: 419 KINSFEGEVAEGEIV 433
+I+ + G +
Sbjct: 490 RIDEAKQYSLNGSFI 504
>MIAB_SYMTH RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 470
Score = 222 bits (565), Expect = 5e-60
Identities = 147/451 (32%), Positives = 233/451 (51%), Gaps = 20/451 (4%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIG 61
+V T GC++N ++SE M + GY + AD+ + NTC+V + + IG
Sbjct: 21 RVHIETFGCQMNEHDSEIMYGILAQMGYVKAQGPDDADLLLFNTCAVRESAVEHAFGRIG 80
Query: 62 RARR---QNPEAIIAVVGCYAQIAPTEVSGIEGV----DVVLGSRNKGEIVYYVNKARDE 114
+ + NP+ II V GC Q+ +V I+ + D++ G+ N + V +AR E
Sbjct: 81 QLKPLKYTNPDLIIGVCGCVPQVEG-QVERIKRMFPYLDLIFGTHNIHRLPELVERARSE 139
Query: 115 GKQQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPK 174
+ V V + + F D+ +A++ I GC++ C YC++P TRG S+ +
Sbjct: 140 RETVVDVWESMGDD-FPDILPAAREGDLKAWVTIMYGCDKHCTYCIVPTTRGKERSRPYE 198
Query: 175 KVLDEIRSLAEHGFKEIILSGIHTASYGVDL-----DEKVTLVDLLEEIEKID-GIERVR 228
+L E++ LA GFKEI L G + +YG DL + DL+E I++ GIER+R
Sbjct: 199 VILAEVQELARQGFKEITLLGQNVNAYGKDLYGRHGEGAFDFGDLIELIDRNSPGIERIR 258
Query: 229 IGSIDPTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRD 288
+ P FT +V +I +K+C FHL +QSG D+ L+RM R Y K+Y +V +R+
Sbjct: 259 FTTNHPKDFTRKMVEQIARAEKVCEWFHLPVQSGSDSVLRRMKRSYNRKQYLRLVGWIRE 318
Query: 289 KIEDVSITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDG 348
I D ITTD+IVGFPG ++ + +F YS R GT A ME+++
Sbjct: 319 LIPDAVITTDIIVGFPGETEEEFQETLSLVEEVQYDAAFMFMYSERAGTPAAQMEDRLSV 378
Query: 349 NKKDERSKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKAD 408
+K ER ++L+E+ +A E +G+V D+L E + V G+TR I V D
Sbjct: 379 PEKKERLQRLMEVQNRIARAKNEARVGKVYDILVEGLDKGKPDVVFGWTRGNILVTFPGD 438
Query: 409 CNVSGKILNVKIN-----SFEGEVAEGEIVL 434
++ G+I+ V+I + EGE+ E + L
Sbjct: 439 ESLRGRIVPVRITRAGTWTLEGELVESPVTL 469
>MIAB_STAAR RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 514
Score = 221 bits (564), Expect = 7e-60
Identities = 141/435 (32%), Positives = 226/435 (51%), Gaps = 12/435 (2%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIGRAR-- 64
T GC++N +++E + GY+ ADV ++NTC++ + K IG +
Sbjct: 74 TYGCQMNAHDTEVIAGILEALGYQATTDINTADVILINTCAIRENAENKVFSEIGNLKHL 133
Query: 65 -RQNPEAIIAVVGCYAQ---IAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVK 120
++ P+ +I V GC +Q + + + VD++ G+ N + + +A V+
Sbjct: 134 KKERPDILIGVCGCMSQEESVVNKILKSYQNVDMIFGTHNIHHLPEILEEAYLSKAMVVE 193
Query: 121 VGAVLKNKVFEDL-KIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDE 179
V + + V E+L K+ E N K A++ I GC++FC YC++P+TRG S+ P+ ++DE
Sbjct: 194 VWSK-EGDVIENLPKVREGNIK--AWVNIMYGCDKFCTYCIVPFTRGKERSRRPEDIIDE 250
Query: 180 IRSLAEHGFKEIILSGIHTASYGVDL-DEKVTLVDLLEEIEKIDGIERVRIGSIDPTFFT 238
+R LA G+KEI L G + SYG DL D + L DLL+ I KI I RVR + P FT
Sbjct: 251 VRELAREGYKEITLLGQNVNSYGKDLQDIEYDLGDLLQAISKI-AIPRVRFTTSHPWDFT 309
Query: 239 EDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITTD 298
+ ++ I + PH HL +QSG +A LK M R+YT + Y D+V ++D+I +V++TTD
Sbjct: 310 DHMIDVISEGGNIVPHIHLPVQSGNNAVLKIMGRKYTRESYLDLVKRIKDRIPNVALTTD 369
Query: 299 VIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSKKL 358
+IVG+P + + + YS R GT A M++ V + K ER ++L
Sbjct: 370 IIVGYPNESEEQFEETLTLYDEVGFEHAYTYLYSQRDGTPAAKMKDNVPLDVKKERLQRL 429
Query: 359 IELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSGKILNV 418
+ + KY G+ + VL E + V GYT VN KA + GK++ V
Sbjct: 430 NKKVGHYSQIAMSKYEGQTVTVLCEGSSKKDDQVLAGYTDKNKLVNFKAPKEMIGKLVEV 489
Query: 419 KINSFEGEVAEGEIV 433
+I+ + G V
Sbjct: 490 RIDEAKQYSLNGSFV 504
>MIAB_STAAB RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 514
Score = 221 bits (564), Expect = 7e-60
Identities = 141/435 (32%), Positives = 226/435 (51%), Gaps = 12/435 (2%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIGRAR-- 64
T GC++N +++E + GY+ ADV ++NTC++ + K IG +
Sbjct: 74 TYGCQMNAHDTEVIAGILEALGYQATTDINTADVILINTCAIRENAENKVFSEIGNLKHL 133
Query: 65 -RQNPEAIIAVVGCYAQ---IAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVK 120
++ P+ +I V GC +Q + + + VD++ G+ N + + +A V+
Sbjct: 134 KKERPDILIGVCGCMSQEESVVNKILKSYQNVDMIFGTHNIHHLPEILEEAYLSKAMVVE 193
Query: 121 VGAVLKNKVFEDL-KIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDE 179
V + + V E+L K+ E N K A++ I GC++FC YC++P+TRG S+ P+ ++DE
Sbjct: 194 VWSK-EGDVIENLPKVREGNIK--AWVNIMYGCDKFCTYCIVPFTRGKERSRRPEDIIDE 250
Query: 180 IRSLAEHGFKEIILSGIHTASYGVDL-DEKVTLVDLLEEIEKIDGIERVRIGSIDPTFFT 238
+R LA G+KEI L G + SYG DL D + L DLL+ I KI I RVR + P FT
Sbjct: 251 VRELAREGYKEITLLGQNVNSYGKDLQDIEYDLGDLLQAISKI-AIPRVRFTTSHPWDFT 309
Query: 239 EDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITTD 298
+ ++ I + PH HL +QSG +A LK M R+YT + Y D+V ++D+I +V++TTD
Sbjct: 310 DHMIDVISEGGNIVPHIHLPVQSGNNAVLKIMGRKYTRESYLDLVKRIKDRIPNVALTTD 369
Query: 299 VIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSKKL 358
+IVG+P + + + YS R GT A M++ V + K ER ++L
Sbjct: 370 IIVGYPNESEEQFEETLTLYDEVGFEHAYTYLYSQRDGTPAAKMKDNVPLDVKKERLQRL 429
Query: 359 IELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSGKILNV 418
+ + KY G+ + VL E + V GYT VN KA + GK++ V
Sbjct: 430 NKKVGHYSQIAMSKYEGQTVTVLCEGSSKKDDQVLAGYTDKNKLVNFKAPKEMIGKLVEV 489
Query: 419 KINSFEGEVAEGEIV 433
+I+ + G V
Sbjct: 490 RIDEAKQYSLNGSFV 504
>MIAB_BACCR RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 509
Score = 221 bits (564), Expect = 7e-60
Identities = 141/441 (31%), Positives = 232/441 (52%), Gaps = 14/441 (3%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIG 61
K T GC++N +++E M F GYE E ADV ++NTC++ + K +G
Sbjct: 67 KFYIRTYGCQMNEHDTEVMAGIFTALGYEPTFSTEDADVVLLNTCAIRENAENKVFGELG 126
Query: 62 RAR---RQNPEAIIAVVGCYAQ---IAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEG 115
+ R+NP+ +I V GC +Q + + + VD+V G+ N + Y + A
Sbjct: 127 HLKSLKRRNPDFLIGVCGCMSQEESVVNKIMQKNQHVDMVFGTHNIHRLPYILKDAMFSK 186
Query: 116 KQQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKK 175
+ V+V + + V E+L + +A++ I GC++FC YC++PYTRG S+ P+
Sbjct: 187 ETVVEVWSK-EGDVIENLP-KVRRGDIKAWVNIMYGCDKFCTYCIVPYTRGKERSRRPED 244
Query: 176 VLDEIRSLAEHGFKEIILSGIHTASYGVDLDE-KVTLVDLLEEIEKIDGIERVRIGSIDP 234
++ EIR LA +G+KEI L G + +YG D ++ + L DL++E+ K+D I R+R + P
Sbjct: 245 IIQEIRHLAANGYKEITLLGQNVNAYGKDFEDIEYGLGDLMDELRKVD-IARIRFTTSHP 303
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
F + ++ + L H HL +QSG LK M R+Y+ + Y ++V +++ I D
Sbjct: 304 RDFDDHLIEVLGKGGNLVEHIHLPVQSGSTDMLKIMARKYSREHYLELVRKIKEAIPDAV 363
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
+TTD+IVGFP + + F YSPR+GT A M++ V K ER
Sbjct: 364 LTTDIIVGFPNETDEQFEETMSLYREVGFDTAFTFIYSPREGTPAAKMKDNVPMEVKKER 423
Query: 355 SKKLIELNRINEKAFAE--KYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVS 412
++L L +N A + +Y G++++VL + E + V GYTR VN A ++
Sbjct: 424 LQRLNTL--VNTLAIEKNSRYKGQIVEVLVDGESKNNPEVLAGYTRTNKLVNFVASKSLI 481
Query: 413 GKILNVKINSFEGEVAEGEIV 433
G+++ VKI + GE+V
Sbjct: 482 GQLVKVKITEAKTWSLNGELV 502
>MIAB_BACC2 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 509
Score = 221 bits (564), Expect = 7e-60
Identities = 140/441 (31%), Positives = 232/441 (52%), Gaps = 14/441 (3%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIG 61
K T GC++N +++E M F GYE E ADV ++NTC++ + K +G
Sbjct: 67 KFYIRTYGCQMNEHDTEVMAGIFTTLGYEPTFSTEDADVVLLNTCAIRENAENKVFGELG 126
Query: 62 RAR---RQNPEAIIAVVGCYAQ---IAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEG 115
+ R+NP+ +I V GC +Q + + + VD+V G+ N + Y + A
Sbjct: 127 HLKSLKRRNPDLLIGVCGCMSQEESVVNKIMQKNQHVDMVFGTHNIHRLPYILKDAMFSK 186
Query: 116 KQQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKK 175
+ V+V + + V E+L + +A++ I GC++FC YC++PYTRG S+ P+
Sbjct: 187 ETVVEVWSK-EGDVIENLP-KVRRGDIKAWVNIMYGCDKFCTYCIVPYTRGKERSRRPED 244
Query: 176 VLDEIRSLAEHGFKEIILSGIHTASYGVDLDE-KVTLVDLLEEIEKIDGIERVRIGSIDP 234
++ EIR LA +G+KEI L G + +YG D ++ + L DL++E+ K+D I R+R + P
Sbjct: 245 IIQEIRHLAANGYKEITLLGQNVNAYGKDFEDVEYGLGDLMDELRKVD-IARIRFTTSHP 303
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
F + ++ + L H HL +QSG LK M R+Y+ + Y ++V +++ I D
Sbjct: 304 RDFDDHLIEVLGKGGNLVEHIHLPVQSGSTDMLKIMARKYSREHYLELVRKIKEAIPDAV 363
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
+TTD+IVGFP + + F YSPR+GT A M++ V K ER
Sbjct: 364 LTTDIIVGFPNETDEQFEETMSLYREVGFDTAFTFIYSPREGTPAAKMKDNVPMEVKKER 423
Query: 355 SKKLIELNRINEKAFAE--KYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVS 412
++L L +N A + +Y G++++VL + E + V GYTR VN A ++
Sbjct: 424 LQRLNTL--VNTLAIEKNSRYKGQIVEVLVDGESKNNPEVLAGYTRTNKLVNFVASKSLI 481
Query: 413 GKILNVKINSFEGEVAEGEIV 433
G+++ VK+ + GE+V
Sbjct: 482 GQLVKVKVTEAKTWSLNGELV 502
>MIAB_CALS8 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 471
Score = 221 bits (563), Expect = 9e-60
Identities = 135/433 (31%), Positives = 217/433 (50%), Gaps = 9/433 (2%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIGRARR- 65
T GC++N ++SE + GY E AD+ + NTC+V + + IG +R
Sbjct: 42 TYGCQMNVHDSEKLAGMLNAMGYVETQNLEEADLIIFNTCAVREHAESRVYGNIGPLKRL 101
Query: 66 --QNPEAIIAVVGCYAQ---IAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVK 120
+ PE II V GC Q +A +D++ G+++ + + KA K
Sbjct: 102 KDKKPELIIGVCGCMPQQLEVAQKLAKVFPFLDIIFGTKSLHKFPQLLYKAITTKKTXXX 161
Query: 121 VGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDEI 180
AF+ I GCN FC+YC++PY RG S+ P++++ EI
Sbjct: 162 XXXXXXXXXXGIPTARREG--VSAFVNIIYGCNNFCSYCIVPYVRGRERSRRPEEIIFEI 219
Query: 181 RSLAEHGFKEIILSGIHTASYGVDLDEKVTLVDLLEEIEKIDGIERVRIGSIDPTFFTED 240
+ LA +G KE+ L G + SYG DL + + LLE++ I+GIER+R + P +++
Sbjct: 220 QQLAANGVKEVTLLGQNVNSYGKDLPDGIPFYKLLEKVNAIEGIERIRFVTSHPKDLSDE 279
Query: 241 VVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITTDVI 300
++ + L+K+C H HL +QSG L++MNR YT ++Y +V L++ I D++ITTD+I
Sbjct: 280 LIFAMRDLEKVCEHIHLPVQSGSTRILRQMNRHYTKEDYLRLVEKLKENIPDIAITTDII 339
Query: 301 VGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSKKLIE 360
VGFPG ++++ + F YS RKGT+A M NQV K ER ++L++
Sbjct: 340 VGFPGETEEDFEDTLDVVRKVEFDSAYTFIYSKRKGTKAAQMPNQVPDEVKHERFQRLVK 399
Query: 361 LNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSGKILNVKI 420
L ++ +G+V ++L + + + G TR VN K K +NVKI
Sbjct: 400 LVEEIALKKNQQMLGKVCEILI-DGYSKRNNLLEGRTRTNKVVNVKCSDEFMYKFVNVKI 458
Query: 421 NSFEGEVAEGEIV 433
GE++
Sbjct: 459 LEASRHWLYGEVI 471
>MIAB_MYCPE RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 491
Score = 221 bits (562), Expect = 1e-59
Identities = 142/435 (32%), Positives = 222/435 (51%), Gaps = 12/435 (2%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIG---RA 63
T GC+ N ++E M GYE + +D+ ++NTC+V + K IG R
Sbjct: 60 TFGCQSNLRDTEVMMGMLELIGYEYNEDVNTSDLVLLNTCAVREHAESKVFADIGILDRI 119
Query: 64 RRQNPEAIIAVVGCYAQ---IAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVK 120
++ NP I V GC AQ + + VD + G+ N I+ + + E V+
Sbjct: 120 KKSNPNFIFGVCGCMAQEEAVVNRILKSNFNVDFIFGTHNVHRILNLLEQVIFEKNLVVE 179
Query: 121 VGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDEI 180
V + N V E+L + NN + F+ + GC++FC YC++P TRG + S+ + +LDE+
Sbjct: 180 VWSHEGN-VIENLPSKRTNN-LKGFVNVMYGCDKFCTYCIVPMTRGKIRSRRKEDILDEV 237
Query: 181 RSLAEHGFKEIILSGIHTASYGVDLD--EKVTLVDLLEEIEKIDGIERVRIGSIDPTFFT 238
+ G+KE+ L G + SYG+D D E +LLE++ K GIERVR + +P FT
Sbjct: 238 HQMISEGYKEVTLIGQNVNSYGIDFDNGENYLFNNLLEDVAKT-GIERVRFTTSNPWNFT 296
Query: 239 EDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITTD 298
+V + + PH HL +QSG + LK+MNR +Y D+V+ +R I + SITTD
Sbjct: 297 RSIVDTMKKYPNIMPHIHLPIQSGDETILKKMNRPMKIGDYIDLVDYIRANIPNCSITTD 356
Query: 299 VIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSKKL 358
+IVGFP KRI F YS R GT A + +++ ++K ER ++L
Sbjct: 357 LIVGFPNETKEQFNKTLELYKRIEFDNAFTFIYSKRDGTVAAIIPDEIPLSEKKERLQEL 416
Query: 359 IELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSGKILNV 418
E+ + K EKY+ +V+DVL + + V +GY+ + VN SG+I+ V
Sbjct: 417 NEMVKTFSKKNNEKYVNKVLDVLVDGPSKKDKTVISGYSPQWKVVNFTGSAK-SGEIVKV 475
Query: 419 KINSFEGEVAEGEIV 433
I S G+++
Sbjct: 476 LITSASRFTLNGKMI 490
>MIAB_GEOKA RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 531
Score = 221 bits (562), Expect = 1e-59
Identities = 137/439 (31%), Positives = 227/439 (51%), Gaps = 10/439 (2%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIG 61
K T GC++N +++E M F+ GYE D E A+V ++NTC++ + K +G
Sbjct: 81 KFYIRTYGCQMNEHDTEVMAGIFMALGYEPTDRPEEANVILLNTCAIRENAENKVFGELG 140
Query: 62 RAR---RQNPEAIIAVVGCYAQ---IAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEG 115
+ NP+ ++ V GC +Q + + + VD++ G+ N + Y +++A
Sbjct: 141 YLKPLKTTNPDLLLGVCGCMSQEESVVKKILKQYQYVDLIFGTHNIHRLPYILHEAYMSK 200
Query: 116 KQQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKK 175
+ V+V + + V E+L + K +A++ I GC++FC YC++PYTRG S+ P+
Sbjct: 201 EMVVEVWSK-EGDVVENLP-KVRKGKIKAWVNIMYGCDKFCTYCIVPYTRGKERSRRPED 258
Query: 176 VLDEIRSLAEHGFKEIILSGIHTASYGVDL-DEKVTLVDLLEEIEKIDGIERVRIGSIDP 234
++ E+R LA G+KEI L G + +YG D D + L DL++E+ KID I R+R + P
Sbjct: 259 IIQEVRQLAAQGYKEITLLGQNVNAYGKDFTDIQYGLGDLMDELRKID-IARIRFTTSHP 317
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
F + ++ + L H HL +QSG LK M R+YT +EY ++V ++ I DV+
Sbjct: 318 RDFDDRLIEVLAKRGNLVEHIHLPVQSGSTEILKMMGRKYTREEYLELVRKIKAAIPDVA 377
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
+TTD+IVGFP + + + F YSPR+GT A NM++ V K ER
Sbjct: 378 LTTDIIVGFPNETDEQFEETLSLYREVEFDSAYTFIYSPREGTPAANMKDNVPMEVKKER 437
Query: 355 SKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSGK 414
K+L +L + ++Y + V GYTR V+ ++ G+
Sbjct: 438 LKRLNDLVQEIAAKKMKQYXXXXXXXXXXXXSKTNPDVLAGYTRKNKLVHFVGPKSLIGQ 497
Query: 415 ILNVKINSFEGEVAEGEIV 433
++NV+I + GE+V
Sbjct: 498 LVNVRITQAKTWTLTGELV 516
>MIAB_BACHK RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 509
Score = 221 bits (562), Expect = 1e-59
Identities = 139/441 (31%), Positives = 233/441 (52%), Gaps = 14/441 (3%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIG 61
K T GC++N +++E M F GYE E ADV ++NTC++ + K +G
Sbjct: 67 KFYIRTYGCQMNEHDTEVMAGIFTALGYEPTFSTEDADVVLLNTCAIRENAENKVFGELG 126
Query: 62 RAR---RQNPEAIIAVVGCYAQ---IAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEG 115
+ R+NP+ +I V GC +Q + + + VD+V G+ N + Y + A
Sbjct: 127 HLKALKRRNPDLLIGVCGCMSQEESVVNKIMQKNQHVDMVFGTHNIHRLPYILKDAMFSK 186
Query: 116 KQQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKK 175
+ V+V + + V E+L + +A++ I GC++FC YC++PYTRG S+ P+
Sbjct: 187 ETVVEVWSK-EGDVIENLP-KVRRGDIKAWVNIMYGCDKFCTYCIVPYTRGKERSRRPED 244
Query: 176 VLDEIRSLAEHGFKEIILSGIHTASYGVDLDE-KVTLVDLLEEIEKIDGIERVRIGSIDP 234
++ EIR LA +G+KEI L G + +YG D ++ + L DL++E+ K+D I R+R + P
Sbjct: 245 IIQEIRHLAANGYKEITLLGQNVNAYGKDFEDIEYGLGDLMDELRKVD-IARIRFTTSHP 303
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
F + ++ + L H HL +QSG LK M R+Y+ + Y ++V +++ I +
Sbjct: 304 RDFDDHLIEVLGKGGNLVEHIHLPVQSGSTEMLKIMARKYSREHYLELVRKIKEAIPNAV 363
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
+TTD+IVGFP + + F YSPR+GT A M++ V K ER
Sbjct: 364 LTTDIIVGFPNETDEQFEETMSLYREVGFDTAFTFIYSPREGTPAAKMKDNVPMEVKKER 423
Query: 355 SKKLIELNRINEKAFA--EKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVS 412
++L L +N+ A ++Y G++++VL + E + V GYTR VN A ++
Sbjct: 424 LQRLNAL--VNKLAIEKNDRYKGQIVEVLVDGESKNNPEVLAGYTRTNKLVNFVAPKSLI 481
Query: 413 GKILNVKINSFEGEVAEGEIV 433
G+++ VK+ + GE+V
Sbjct: 482 GQLVKVKVTDAKTWSLNGELV 502
>MIAB_BACCZ RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 509
Score = 221 bits (562), Expect = 1e-59
Identities = 139/441 (31%), Positives = 233/441 (52%), Gaps = 14/441 (3%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIG 61
K T GC++N +++E M F GYE E ADV ++NTC++ + K +G
Sbjct: 67 KFYIRTYGCQMNEHDTEVMAGIFTALGYEPTFSTEDADVVLLNTCAIRENAENKVFGELG 126
Query: 62 RAR---RQNPEAIIAVVGCYAQ---IAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEG 115
+ R+NP+ +I V GC +Q + + + VD+V G+ N + Y + A
Sbjct: 127 HLKALKRRNPDLLIGVCGCMSQEESVVNKIMQKNQHVDMVFGTHNIHRLPYILKDAMFSK 186
Query: 116 KQQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKK 175
+ V+V + + V E+L + +A++ I GC++FC YC++PYTRG S+ P+
Sbjct: 187 ETVVEVWSK-EGDVIENLP-KVRRGDIKAWVNIMYGCDKFCTYCIVPYTRGKERSRRPED 244
Query: 176 VLDEIRSLAEHGFKEIILSGIHTASYGVDLDE-KVTLVDLLEEIEKIDGIERVRIGSIDP 234
++ EIR LA +G+KEI L G + +YG D ++ + L DL++E+ K+D I R+R + P
Sbjct: 245 IIQEIRHLAANGYKEITLLGQNVNAYGKDFEDIEYGLGDLMDELRKVD-IARIRFTTSHP 303
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
F + ++ + L H HL +QSG LK M R+Y+ + Y ++V +++ I +
Sbjct: 304 RDFDDHLIEVLGKGGNLVEHIHLPVQSGSTEMLKIMARKYSREHYLELVRKIKEAIPNAV 363
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
+TTD+IVGFP + + F YSPR+GT A M++ V K ER
Sbjct: 364 LTTDIIVGFPNETDEQFEETMSLYREVGFDTAFTFIYSPREGTPAAKMKDNVPMEVKKER 423
Query: 355 SKKLIELNRINEKAFA--EKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVS 412
++L L +N+ A ++Y G++++VL + E + V GYTR VN A ++
Sbjct: 424 LQRLNAL--VNKLAIEKNDRYKGQIVEVLVDGESKNNPEVLAGYTRTNKLVNFVAPKSLI 481
Query: 413 GKILNVKINSFEGEVAEGEIV 433
G+++ VK+ + GE+V
Sbjct: 482 GQLVKVKVTDAKTWSLNGELV 502
>MIAB_BACC7 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 509
Score = 221 bits (562), Expect = 1e-59
Identities = 139/441 (31%), Positives = 233/441 (52%), Gaps = 14/441 (3%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIG 61
K T GC++N +++E M F GYE E ADV ++NTC++ + K +G
Sbjct: 67 KFYIRTYGCQMNEHDTEVMAGIFTALGYEPTFSTEEADVVLLNTCAIRENAENKVFGELG 126
Query: 62 RAR---RQNPEAIIAVVGCYAQ---IAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEG 115
+ R+NP+ +I V GC +Q + + + VD+V G+ N + Y + A
Sbjct: 127 HLKALKRRNPDLLIGVCGCMSQEESVVNKIMQKNQHVDMVFGTHNIHRLPYILKDAMFSK 186
Query: 116 KQQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKK 175
+ V+V + + V E+L + +A++ I GC++FC YC++PYTRG S+ P+
Sbjct: 187 ETVVEVWSK-EGDVIENLP-KVRRGDIKAWVNIMYGCDKFCTYCIVPYTRGKERSRRPED 244
Query: 176 VLDEIRSLAEHGFKEIILSGIHTASYGVDLDE-KVTLVDLLEEIEKIDGIERVRIGSIDP 234
++ EIR LA +G+KEI L G + +YG D ++ + L DL++E+ K+D I R+R + P
Sbjct: 245 IIQEIRHLAANGYKEITLLGQNVNAYGKDFEDIEYGLGDLMDELRKVD-IARIRFTTSHP 303
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
F + ++ + L H HL +QSG LK M R+Y+ + Y ++V +++ I +
Sbjct: 304 RDFDDHLIEVLGKGGNLVEHIHLPVQSGSTEMLKIMARKYSREHYLELVRKIKEAIPNAV 363
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
+TTD+IVGFP + + F YSPR+GT A M++ V K ER
Sbjct: 364 LTTDIIVGFPNETDEQFEETMSLYREVGFDTAFTFIYSPREGTPAAKMKDNVPMEVKKER 423
Query: 355 SKKLIELNRINEKAFAE--KYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVS 412
++L L +N+ A + +Y G++++VL + E + V GYTR VN A ++
Sbjct: 424 LQRLNAL--VNKLAIEKNNRYKGQIVEVLVDGESKNNPEVLAGYTRTNKLVNFVAPKSLI 481
Query: 413 GKILNVKINSFEGEVAEGEIV 433
G+++ VK+ + GE+V
Sbjct: 482 GQLVKVKVTDAKTWSLNGELV 502
>MIAB_BACC1 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 509
Score = 221 bits (562), Expect = 1e-59
Identities = 139/441 (31%), Positives = 233/441 (52%), Gaps = 14/441 (3%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIG 61
K T GC++N +++E M F GYE E ADV ++NTC++ + K +G
Sbjct: 67 KFYIRTYGCQMNEHDTEVMAGIFTALGYEPTFSTEEADVVLLNTCAIRENAENKVFGELG 126
Query: 62 RAR---RQNPEAIIAVVGCYAQ---IAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEG 115
+ R+NP+ +I V GC +Q + + + VD+V G+ N + Y + A
Sbjct: 127 HLKALKRRNPDLLIGVCGCMSQEESVVNKIMQKNQHVDMVFGTHNIHRLPYILKDAMFSK 186
Query: 116 KQQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKK 175
+ V+V + + V E+L + +A++ I GC++FC YC++PYTRG S+ P+
Sbjct: 187 ETVVEVWSK-EGDVIENLP-KVRRGDIKAWVNIMYGCDKFCTYCIVPYTRGKERSRRPED 244
Query: 176 VLDEIRSLAEHGFKEIILSGIHTASYGVDLDE-KVTLVDLLEEIEKIDGIERVRIGSIDP 234
++ EIR LA +G+KEI L G + +YG D ++ + L DL++E+ K+D I R+R + P
Sbjct: 245 IIQEIRHLAANGYKEITLLGQNVNAYGKDFEDIEYGLGDLMDELRKVD-IARIRFTTSHP 303
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
F + ++ + L H HL +QSG LK M R+Y+ + Y ++V +++ I +
Sbjct: 304 RDFDDHLIEVLGKGGNLVEHIHLPVQSGSTEMLKIMARKYSREHYLELVRKIKEAIPNAV 363
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
+TTD+IVGFP + + F YSPR+GT A M++ V K ER
Sbjct: 364 LTTDIIVGFPNETDEQFEETMSLYREVGFDTAFTFIYSPREGTPAAKMKDNVPMEVKKER 423
Query: 355 SKKLIELNRINEKAFAE--KYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVS 412
++L L +N+ A + +Y G++++VL + E + V GYTR VN A ++
Sbjct: 424 LQRLNAL--VNKLAIEKNNRYKGQIVEVLVDGESKNNPEVLAGYTRTNKLVNFVAPKSLI 481
Query: 413 GKILNVKINSFEGEVAEGEIV 433
G+++ VK+ + GE+V
Sbjct: 482 GQLVKVKVTDAKTWSLNGELV 502
>MIAB_BACC0 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 509
Score = 221 bits (562), Expect = 1e-59
Identities = 139/441 (31%), Positives = 233/441 (52%), Gaps = 14/441 (3%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIG 61
K T GC++N +++E M F GYE E ADV ++NTC++ + K +G
Sbjct: 67 KFYIRTYGCQMNEHDTEVMAGIFTALGYEPTFSTEDADVVLLNTCAIRENAENKVFGELG 126
Query: 62 RAR---RQNPEAIIAVVGCYAQ---IAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEG 115
+ R+NP+ +I V GC +Q + + + VD+V G+ N + Y + A
Sbjct: 127 HLKSLKRRNPDLLIGVCGCMSQEESVVNKIMQKNQHVDMVFGTHNIHRLPYILKDAMFSK 186
Query: 116 KQQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKK 175
+ V+V + + V E+L + +A++ I GC++FC YC++PYTRG S+ P+
Sbjct: 187 ETVVEVWSK-EGDVIENLP-KVRRGDIKAWVNIMYGCDKFCTYCIVPYTRGKERSRRPED 244
Query: 176 VLDEIRSLAEHGFKEIILSGIHTASYGVDLDE-KVTLVDLLEEIEKIDGIERVRIGSIDP 234
++ EIR LA +G+KEI L G + +YG D ++ + L DL++E+ K+D I R+R + P
Sbjct: 245 IIQEIRHLAANGYKEITLLGQNVNAYGKDFEDIEYGLGDLMDELRKVD-IARIRFTTSHP 303
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
F + ++ + L H HL +QSG LK M R+Y+ + Y ++V +++ I +
Sbjct: 304 RDFDDHLIEVLGKGGNLVEHIHLPVQSGSTEMLKIMARKYSREHYLELVRKIKEAIPNAV 363
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
+TTD+IVGFP + + F YSPR+GT A M++ V K ER
Sbjct: 364 LTTDIIVGFPNETDEQFEETMSLYREVGFDTAFTFIYSPREGTPAAKMKDNVPMEVKKER 423
Query: 355 SKKLIELNRINEKAFA--EKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVS 412
++L L +N+ A ++Y G++++VL + E + V GYTR VN A ++
Sbjct: 424 LQRLNAL--VNKLAIEKNDRYKGQIVEVLVDGESKNNPEVLAGYTRTNKLVNFVAPKSLI 481
Query: 413 GKILNVKINSFEGEVAEGEIV 433
G+++ VK+ + GE+V
Sbjct: 482 GQLVKVKVTDAKTWSLNGELV 502
>MIAB_BACAH RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 509
Score = 221 bits (562), Expect = 1e-59
Identities = 139/441 (31%), Positives = 233/441 (52%), Gaps = 14/441 (3%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIG 61
K T GC++N +++E M F GYE E ADV ++NTC++ + K +G
Sbjct: 67 KFYIRTYGCQMNEHDTEVMAGIFTALGYEPTFSTEDADVVLLNTCAIRENAENKVFGELG 126
Query: 62 RAR---RQNPEAIIAVVGCYAQ---IAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEG 115
+ R+NP+ +I V GC +Q + + + VD+V G+ N + Y + A
Sbjct: 127 HLKSLKRRNPDLLIGVCGCMSQEESVVNKIMQKNQHVDMVFGTHNIHRLPYILKDAMFSK 186
Query: 116 KQQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKK 175
+ V+V + + V E+L + +A++ I GC++FC YC++PYTRG S+ P+
Sbjct: 187 ETVVEVWSK-EGDVIENLP-KVRRGDIKAWVNIMYGCDKFCTYCIVPYTRGKERSRRPED 244
Query: 176 VLDEIRSLAEHGFKEIILSGIHTASYGVDLDE-KVTLVDLLEEIEKIDGIERVRIGSIDP 234
++ EIR LA +G+KEI L G + +YG D ++ + L DL++E+ K+D I R+R + P
Sbjct: 245 IIQEIRHLAANGYKEITLLGQNVNAYGKDFEDIEYGLGDLMDELRKVD-IARIRFTTSHP 303
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
F + ++ + L H HL +QSG LK M R+Y+ + Y ++V +++ I +
Sbjct: 304 RDFDDHLIEVLGKGGNLVEHIHLPVQSGSTEMLKIMARKYSREHYLELVRKIKEAIPNAV 363
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
+TTD+IVGFP + + F YSPR+GT A M++ V K ER
Sbjct: 364 LTTDIIVGFPNETDEQFEETMSLYREVGFDTAFTFIYSPREGTPAAKMKDNVPMEVKKER 423
Query: 355 SKKLIELNRINEKAFA--EKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVS 412
++L L +N+ A ++Y G++++VL + E + V GYTR VN A ++
Sbjct: 424 LQRLNAL--VNKLAIEKNDRYKGQIVEVLVDGESKNNPEVLAGYTRTNKLVNFVAPKSLI 481
Query: 413 GKILNVKINSFEGEVAEGEIV 433
G+++ VK+ + GE+V
Sbjct: 482 GQLVKVKVTDAKTWSLNGELV 502
>MIAB_DESRM RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 456
Score = 220 bits (561), Expect = 2e-59
Identities = 126/433 (29%), Positives = 219/433 (50%), Gaps = 9/433 (2%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIGRARR- 65
+ GC++N ++E++ GY E AD+ ++NTC V + K ++GR R+
Sbjct: 23 SFGCQMNERDAESLAGMLEDLGYCPTSAQEEADIILLNTCCVRETAESKVFGLLGRLRKL 82
Query: 66 --QNPEAIIAVVGCYAQ---IAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVK 120
P+ I+ V GC +Q A VD++ G+ N E+ +++ ++ + ++
Sbjct: 83 KVAKPDLILGVCGCMSQQEDAAKRIRRSFPFVDLIFGTHNIHELPRMIHQVQENHEAVLE 142
Query: 121 VGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDEI 180
V A K + E + ++ +K +A++ I GCN FC YC++PY RG S+ P+ ++DEI
Sbjct: 143 VWATEKG-ITESIPVKR-KDKLKAWVTIMYGCNNFCTYCIVPYVRGRERSRQPEDIIDEI 200
Query: 181 RSLAEHGFKEIILSGIHTASYGVDLDEKVTLVDLLEEIEKIDGIERVRIGSIDPTFFTED 240
+ L + G+KE+ L G + SYG D DLL I+ I G+ERVR + P F +
Sbjct: 201 KELVQEGYKEVTLLGQNVNSYGKDFKNNYRFADLLMAIDDITGLERVRFMTSHPRDFDQR 260
Query: 241 VVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITTDVI 300
++ + + KK+C H+HL Q+G + LK MNR YT + Y +++ +++++ + SIT D++
Sbjct: 261 LIEVVASAKKVCEHYHLPAQAGSNRVLKMMNRGYTREHYLELIRKIKERVPNASITADLM 320
Query: 301 VGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSKKLIE 360
VGFPG +K++ F Y+ R GT A +E QV K ER ++LIE
Sbjct: 321 VGFPGETEEDFQETLDLVKQVRYDSAFTFVYNIRSGTPAAKLE-QVSEEVKSERIQRLIE 379
Query: 361 LNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSGKILNVKI 420
L + + + + G TR V + ++ G+++ ++I
Sbjct: 380 LQNLISLENNQXXXXXXXXXXXXXXTKTNPDLLAGRTRTNKLVVFQGSGHLPGQLVQIRI 439
Query: 421 NSFEGEVAEGEIV 433
+ EGE+V
Sbjct: 440 TKGRPNLLEGEVV 452
>MIAB_METI4 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 467
Score = 220 bits (560), Expect = 2e-59
Identities = 127/438 (28%), Positives = 226/438 (51%), Gaps = 10/438 (2%)
Query: 3 VAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIGR 62
V T GC++N +SE + + F+ GY++ + AD+ ++NTCSV M ++K+ +
Sbjct: 4 VFIKTFGCQMNVRDSEQVLQDFIERGYQIASSEKWADIILINTCSVRAMAEEKAIDKLAS 63
Query: 63 ---ARRQNPEAIIAVVGCYAQIAPTEVSG-IEGVDVVLGSRNKGEIVYYVNKARDEGKQQ 118
A+++NP ++ ++GC AQ E++ VD+VLG++ ++ + +
Sbjct: 64 LKTAKKKNPNLVLGIIGCMAQNRGREIAEKYRFVDLVLGTQKFHKVAEIADNLLKNPDRS 123
Query: 119 VKVGAVLKNKVFEDLKIEEYNNKTR--AFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
+ K + + + + K + A++ I GC+ C++C++P TRG S+ ++
Sbjct: 124 SSYVDLSKEEAAHNAINKHLSTKAQPIAYVSIMQGCSMHCSFCIVPTTRGEERSRPIDEI 183
Query: 177 LDEIRSLAEHGFKEIILSGIHTASYGVD----LDEKVTLVDLLEEIEKIDGIERVRIGSI 232
+E++ LAE KEI+L G YG + K V LLE++ I+ I+R+R S
Sbjct: 184 FEEVKRLAETSVKEIVLLGQIVNRYGAKEFPWVKGKSPFVQLLEKLSTIEEIKRIRFTSP 243
Query: 233 DPTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIED 292
P F ED++ + + +LC H HL +QSG D LK M R Y+ ++ +V+ LR I
Sbjct: 244 HPLGFKEDLIAALRDIPQLCEHVHLPVQSGSDKILKAMRRGYSRSKFLSLVDKLRKAIPQ 303
Query: 293 VSITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKD 352
++++TD+IVG+PG L + IF+YS R+GT A ++ +Q+ K
Sbjct: 304 LALSTDIIVGYPGETEEDFQQTCSLLNEVRFDNAFIFRYSAREGTTAASLGDQLSEEVKF 363
Query: 353 ERSKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVS 412
ER+ +L+E+ A+K++G+V+++L E E + + + G TR V +
Sbjct: 364 ERNYRLLEIQNKITMEKAQKWVGQVVEILVEGESKKNASKFQGRTRTNHLVIIPKNERWR 423
Query: 413 GKILNVKINSFEGEVAEG 430
G+ L V+I G G
Sbjct: 424 GEFLPVRIVETTGHTFYG 441
>MIAB_OCEIH RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 519
Score = 219 bits (559), Expect = 3e-59
Identities = 134/426 (31%), Positives = 219/426 (51%), Gaps = 10/426 (2%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIG 61
K T GC++N +++E M GYE E AD+ ++NTC++ + K IG
Sbjct: 77 KFLIRTYGCQMNEHDTEVMAGILSEMGYESTTVTEEADIILLNTCAIRENAENKVFGEIG 136
Query: 62 RARR---QNPEAIIAVVGCYAQ---IAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEG 115
+ +NP+ II V GC +Q + + + +D++ G+ N + + V +A G
Sbjct: 137 HLKPLKLENPDLIIGVCGCMSQEESVVDRILQKHQHIDLIFGTHNIHRLPHLVKEALF-G 195
Query: 116 KQQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKK 175
K+ + + + E+L + K +A++ I GC++FC YC++P TRG S+ PK
Sbjct: 196 KEMIVEVWSKEGDIIENLP-KARKGKIKAWVNIMYGCDKFCTYCIVPMTRGKERSRRPKD 254
Query: 176 VLDEIRSLAEHGFKEIILSGIHTASYGVDLDE-KVTLVDLLEEIEKIDGIERVRIGSIDP 234
++ E+R L G++E+ L G + +YG D ++ + L DL+ +I KID I RVR + P
Sbjct: 255 IIQEVRHLVAQGYQEVTLLGQNVNAYGKDFEDIEYGLGDLMNDIHKID-IPRVRFTTSHP 313
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
F + ++ + L H HL +QSG LK+MNR+YT +EY ++V +R + + +
Sbjct: 314 RDFDDRLIEVLAQGGNLLDHIHLPVQSGSSEILKKMNRKYTREEYLELVRKIRIAMPNAT 373
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
+TTD+IVGFP ++ + + F YSPR+GT A ++ V K +R
Sbjct: 374 LTTDIIVGFPNETEEQFEETMTLVEEVGFEAAYTFIYSPREGTPAARKKDDVPEEVKKQR 433
Query: 355 SKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSGK 414
+L EL A + Y G+ + VL E E + V GYT VN K + GK
Sbjct: 434 LYRLNELVNKQSAASMKDYAGKKVKVLVEGESKKDPEVLAGYTEKNKLVNFKGPKSSIGK 493
Query: 415 ILNVKI 420
I+ V+I
Sbjct: 494 IVEVEI 499
>MIAB_BACA2 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 509
Score = 219 bits (558), Expect = 4e-59
Identities = 132/426 (30%), Positives = 224/426 (52%), Gaps = 10/426 (2%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIG 61
K T GC++N +++E M F+ GYE + + A+V ++NTC++ + K +G
Sbjct: 67 KFYIRTYGCQMNEHDTEVMAGIFMALGYEATNSVDDANVILLNTCAIRENAENKVFGELG 126
Query: 62 RAR---RQNPEAIIAVVGCYAQ---IAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEG 115
+ + NP+ I+ V GC +Q + + VD++ G+ N + +++A
Sbjct: 127 HLKALKKNNPDLILGVCGCMSQEESVVNRILKKHPFVDMIFGTHNIHRLPELLSEAYLSK 186
Query: 116 KQQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKK 175
+ ++V + + V E+L + N K + ++ I GC++FC YC++PYTRG S+ P++
Sbjct: 187 EMVIEVWSK-EGDVIENLP-KVRNGKIKGWVNIMYGCDKFCTYCIVPYTRGKERSRRPEE 244
Query: 176 VLDEIRSLAEHGFKEIILSGIHTASYGVDLDEKVT-LVDLLEEIEKIDGIERVRIGSIDP 234
++ E+R LA G+KEI L G + +YG D ++ L DL++E+ KID I R+R + P
Sbjct: 245 IIQEVRRLASEGYKEITLLGQNVNAYGKDFEDMTYGLGDLMDELRKID-IPRIRFTTSHP 303
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
F + ++ + L H HL +QSG A LK M R+Y + Y ++V ++ + + S
Sbjct: 304 RDFDDHLIEVLAKGGNLLDHIHLPVQSGSSAMLKMMARKYDRERYLELVGKIKAAMPNAS 363
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
+TTD+IVGFP + + + F YSPR+GT A M++ V K ER
Sbjct: 364 LTTDIIVGFPNETDEQFEETLSLYREVEFDSAYTFIYSPREGTPAAKMKDNVPMRVKKER 423
Query: 355 SKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSGK 414
++L +L + ++Y G ++VL E E + + GYT VN K + GK
Sbjct: 424 LQRLNDLVKEISAKKMKEYEGRTVEVLVEGESKNNPDILAGYTEKSKLVNFKGPKDAIGK 483
Query: 415 ILNVKI 420
I+ VKI
Sbjct: 484 IVRVKI 489
>MIAB_AQUAE RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 440
Score = 219 bits (558), Expect = 4e-59
Identities = 140/441 (31%), Positives = 225/441 (51%), Gaps = 20/441 (4%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIG 61
K T GC++N +SE + GYE D E AD+ ++NTC++ D+K +G
Sbjct: 4 KFFIKTFGCQMNFNDSERIRGLLKTIGYEQTDNWEEADLIILNTCTIREKPDQKVLSHLG 63
Query: 62 ---RARRQNPEAIIAVVGCYAQIAPTE-VSGIEGVDVVLGSRNKGEIVYYVNKARDEGKQ 117
+ + +NP+A+IAV GC AQ E V +D++ S N ++ +N+A+
Sbjct: 64 EYKKIKEKNPKALIAVAGCLAQRTGWELVKKAPVIDIMFSSFNMHQLPELINQAQ----A 119
Query: 118 QVKVGAVLKNKVFEDLKIEEY----NNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDP 173
K A+L ++ KI EY +NK A++ I GC++ C YC++P TRG S+
Sbjct: 120 GYKAIAILDELPQDEDKIWEYPVERDNKYCAYVTIIKGCDKNCTYCVVPRTRGKERSRAL 179
Query: 174 KKVLDEIRSLAEHGFKEIILSGIHTASYGVDLDEKVTLVDLLEEIEKIDGIERVRIGSID 233
+LDE++ L + G +EI L G + ++G D ++ + +LL ++ KIDG+ER+R +
Sbjct: 180 HSILDEVKRLVDDGVREIHLLGQNVTAWGKDFEKPIPFSELLYQVSKIDGVERIRFTTGH 239
Query: 234 PTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDV 293
P T+D++ + + ++C HL Q+G + L M+R YT ++D+
Sbjct: 240 PRDLTDDIIEAMADIPQVCNALHLPFQAGSNRILALMDRGYTXXXXXXXXXXXXXXVKDI 299
Query: 294 SITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDE 353
+++TDVIVGFP LK++ + FK+SPR GT A ME Q+ + K E
Sbjct: 300 AMSTDVIVGFPTETEEDFEHTLDVLKKVRFEQVFSFKFSPRPGTPAAEMEGQIPDDVKTE 359
Query: 354 RSKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSG 413
R ++L+EL + A+KY G V +VL EEE +G G T + K D ++ G
Sbjct: 360 RMRRLLELQKSILSEIAKKYEGTVQEVLVEEE---KNGELIGRTTTNKWASFKGDPSLLG 416
Query: 414 KILNVKIN-----SFEGEVAE 429
S EGE+ E
Sbjct: 417 XXXXXXXXXSSPFSLEGELLE 437
>MIAB_BACP2 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 508
Score = 219 bits (557), Expect = 5e-59
Identities = 137/440 (31%), Positives = 229/440 (52%), Gaps = 12/440 (2%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIG 61
K T GC++N +++E M F+ GYE + E A+V ++NTC++ + K +G
Sbjct: 66 KFYIRTYGCQMNEHDTEVMAGIFMALGYEPTNSTEDANVILLNTCAIRENAENKVFGELG 125
Query: 62 RAR---RQNPEAIIAVVGCYAQ---IAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEG 115
+ R+ P+ I+ V GC +Q + + VD++ G+ N + +++
Sbjct: 126 HLKALKREKPDLILGVCGCMSQEESVVNRILKKHPFVDLIFGTHNIHRLPELLSECYLSK 185
Query: 116 KQQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKK 175
+ ++V + + V E+L K + ++ I GC++FC YC++PYTRG S+ P +
Sbjct: 186 EMVIEVWSK-EGDVIENLP-RARQGKIKGWVNIMYGCDKFCTYCIVPYTRGKERSRRPDE 243
Query: 176 VLDEIRSLAEHGFKEIILSGIHTASYGVDLDE-KVTLVDLLEEIEKIDGIERVRIGSIDP 234
++ E+R LA G+KEI L G + +YG D ++ + L L++E+ KID I R+R + P
Sbjct: 244 IIQEVRRLAAEGYKEITLLGQNVNAYGKDFEDMEYGLGHLMDELRKID-IPRIRFTTSHP 302
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
F + ++ + L H HL +QSG + LK M R+Y + Y D+V +++ + + S
Sbjct: 303 RDFDDHLIEVLAKGGNLLDHIHLPVQSGSSSVLKLMARKYDRERYLDLVRKIKEAMPNAS 362
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
+TTD+IVGFP + + + F YSPR+GT A M++ V + K ER
Sbjct: 363 LTTDIIVGFPNETDEQFEETLSLYREVEFDAAYTFIYSPREGTPAAKMQDNVPMHVKKER 422
Query: 355 SKKLIEL-NRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSG 413
++L L N I+ K E Y G+ ++VL E E + + GYT VN KA G
Sbjct: 423 LQRLNALVNEISAKKMKE-YEGQTVEVLVEGESKNNPEILAGYTSKSKLVNFKAPKEAIG 481
Query: 414 KILNVKINSFEGEVAEGEIV 433
I+ VKI + +GE+V
Sbjct: 482 NIVKVKITQAKTWSLDGEMV 501
>MIAB_FUSNN RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 435
Score = 218 bits (555), Expect = 8e-59
Identities = 143/447 (31%), Positives = 227/447 (50%), Gaps = 29/447 (6%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIG 61
K + T GC++N ES + + F GY+V + + AD +NTC+V + + QI
Sbjct: 3 KASIITYGCQMNVNESAKIKKIFQNLGYDVTEEIDNADAVFLNTCTVR---EGAATQIFX 59
Query: 62 RARRQNPE-----AIIAVVGCYAQIAPTE-VSGIEGVDVVLGSRNKGEIVYYVNKARDEG 115
II V GC+AQ E V +D+V+G++N G I + K +
Sbjct: 60 XXXXXXXXXXXRGTIIGVTGCFAQEQGEELVKKFPIIDIVMGNQNIGRIPQAIEKIENNE 119
Query: 116 KQQVKVGAVLKNKVFEDLKIE-------EYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSV 168
++V+ D + E E+ + A + I GCN FC +C++PY RG
Sbjct: 120 ST---------HEVYTDNEDELPPRLDAEFGSDQTASISITYGCNNFCTFCIVPYVRGRE 170
Query: 169 CSKDPKKVLDEIRSLAEHGFKEIILSGIHTASYGVDLDEKVTLVDLLEEIEKIDGIERVR 228
S ++++ ++ + G KEI+L G + SYG D LL+EI K++G VR
Sbjct: 171 RSVPLEEIVKDVEQYVKKGAKEIVLLGQNVNSYGKDFKNGDNFAKLLDEICKVEGDYIVR 230
Query: 229 IGSIDPTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRD 288
S P FT+DV+ I KK+ HL LQSG LK+M R YT ++Y +V+ ++
Sbjct: 231 FVSPHPRDFTDDVIEVIAKNKKISKCLHLPLQSGSSQILKKMRRGYTKEKYLALVDKIKS 290
Query: 289 KIEDVSITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDG 348
KI V++T D+IVGFPG +++++ +++F YS RKGT+A M+NQ++
Sbjct: 291 KIPGVALTADIIVGFPGETEEDFLDTIDVVQKVSFDNSYMFMYSIRKGTKAATMDNQIEE 350
Query: 349 NKKDERSKKLIELNRINEKAFAE--KYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAK 406
+ K ER ++L+E+ N+ +F E KY G ++ VL E + V +G T V +
Sbjct: 351 SVKKERLQRLMEVQ--NKCSFYESSKYKGRIVKVLVEGPSKKNKEVLSGRTSTNKIVLFR 408
Query: 407 ADCNVSGKILNVKINSFEGEVAEGEIV 433
+ + G+ +NVKIN + GEIV
Sbjct: 409 GNLALKGQFINVKINECKTWTLYGEIV 435
>MIAB_CLOD6 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 478
Score = 217 bits (553), Expect = 1e-58
Identities = 132/423 (31%), Positives = 227/423 (53%), Gaps = 13/423 (3%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIGRARR- 65
T GC++N ++SE + GY++ E +D+ + NTC+V + K +G+ +
Sbjct: 45 TFGCQMNEHDSEKLCSMLEEMGYQMSMMVEESDLIIYNTCAVRENAELKVYGNLGQLKHL 104
Query: 66 --QNPEAIIAVVGCYAQIAPTEVSGI----EGVDVVLGSRNKGEIVYYVNKARDEGKQQV 119
+NP+ IAV GC Q P V + + VD++ G+ N + + ++ + K V
Sbjct: 105 KGKNPDMKIAVCGCMMQ-QPHVVEELRKKYKHVDLIFGTHNLYKFPQLLTESINSDKMLV 163
Query: 120 KVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDE 179
V V +V E L+ + +AF+ I GCN FC YC++PYTRG S+ P+ +++E
Sbjct: 164 DVWDV-DGEVIEGLRSNR-KFELKAFVNIMYGCNNFCTYCIVPYTRGRERSRTPEDIINE 221
Query: 180 IRSLAEHGFKEIILSGIHTASYGVDLDEKVTLVDLLEEIEKIDGIERVRIGSIDPTFFTE 239
I+ L +G KEI L G + SYG L+ V+ +LL ++ I+GIERVR + P ++
Sbjct: 222 IKELVANGTKEITLLGQNVDSYGKTLENPVSFSELLRKVNDIEGIERVRFMTSHPKDISD 281
Query: 240 DVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITTDV 299
+V+ I K+C HL +Q G + LK+MNR YT + Y +I+ + ++ ++ +TD+
Sbjct: 282 EVIYAIRDCDKVCEFLHLPIQCGSSSLLKKMNRHYTKEYYLEIIEKAKKEVPGIAFSTDL 341
Query: 300 IVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSKKLI 359
++GFPG ++++ F YS R+GT A MENQ+ + K +R +++
Sbjct: 342 MIGFPGETEEDLLDTLDVVEKVRYDSAFTFIYSKRQGTPAAKMENQIPEDIKHDRFNRVL 401
Query: 360 E-LNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAK-ADCNVSGKILN 417
E +NRI+ + + Y +++VL E + + G TR VN + + ++ GK++
Sbjct: 402 EAVNRISAE-INDGYKDRIVEVLVEGRSKNNENKFAGRTRQNKLVNFEGGNDDLIGKLVM 460
Query: 418 VKI 420
VKI
Sbjct: 461 VKI 463
>MIAB_BACAN RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 509
Score = 217 bits (553), Expect = 1e-58
Identities = 137/441 (31%), Positives = 232/441 (52%), Gaps = 14/441 (3%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIG 61
K T GC++N +++E M F GYE E ADV ++NTC++ + K +G
Sbjct: 67 KFYIRTYGCQMNEHDTEVMAGIFTALGYEPTFSTEDADVVLLNTCAIRENAENKVFGELG 126
Query: 62 RAR---RQNPEAIIAVVGCYAQ---IAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEG 115
+ R+N + +I V GC +Q + + + VD+V G+ N + Y + A
Sbjct: 127 HLKSLKRRNSDLLIGVCGCMSQEESVVNKIMQKNQHVDMVFGTHNIHRLPYILKDAMFSK 186
Query: 116 KQQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKK 175
+ V+V + + V E+L + +A++ I GC++FC YC++PYTRG S+ P+
Sbjct: 187 ETVVEVWSK-EGDVIENLP-KVRRGDIKAWVNIMYGCDKFCTYCIVPYTRGKERSRRPED 244
Query: 176 VLDEIRSLAEHGFKEIILSGIHTASYGVDLDE-KVTLVDLLEEIEKIDGIERVRIGSIDP 234
++ EIR LA +G+KEI L G + +YG D ++ + L DL++E+ K+D I R+R + P
Sbjct: 245 IIQEIRHLAANGYKEITLLGQNVNAYGKDFEDIEYGLGDLMDELRKVD-IARIRFTTSHP 303
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
F + ++ + L H HL +QSG LK M R+Y+ + Y ++V +++ I +
Sbjct: 304 RDFDDHLIEVLGKGGNLVEHIHLPVQSGSTEMLKIMARKYSREHYLELVRKIKEAIPNAV 363
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
+TTD+IVGFP + + F YSPR+GT A M++ V K ER
Sbjct: 364 LTTDIIVGFPNETDEQFEETMSLYREVGFDTAFTFIYSPREGTPAAKMKDNVPMEVKKER 423
Query: 355 SKKLIELNRINEKAFA--EKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVS 412
++L L +N+ A ++Y G++++VL + E + V GYTR VN A ++
Sbjct: 424 LQRLNAL--VNKLAIEKNDRYKGQIVEVLVDGESKNNPEVLAGYTRTNKLVNFVAPKSLI 481
Query: 413 GKILNVKINSFEGEVAEGEIV 433
G+++ +K+ + GE+V
Sbjct: 482 GQLVKIKVTDAKTWSLNGELV 502
>MIAB_SYNWW RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 445
Score = 216 bits (549), Expect = 4e-58
Identities = 136/436 (31%), Positives = 215/436 (49%), Gaps = 6/436 (1%)
Query: 1 MKVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
+K T GC++N +SE + G+ + AD+ V NTCSV + + K +
Sbjct: 9 LKYRILTYGCQMNVRDSETIAGLLEGSGFNQAEDLSEADLIVFNTCSVRHSAENKVYGKL 68
Query: 61 GRA---RRQNPEAIIAVVGCYAQIAPTEVS-GIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
G +++ PE +IA GC AQ+ GVDVV G+ N E+ Y + +A+++
Sbjct: 69 GEIASLKKKRPELLIAFGGCMAQLPEVRQKLKKRGVDVVFGTHNIHELPYLIARAKEKRS 128
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
+V + + E L + AF+ I GCN FC+YC++PYTRG S+ +
Sbjct: 129 PVFEVWEK-EGSIVEPLPSCRKPGLS-AFVNIMFGCNNFCSYCIVPYTRGRERSRKADDI 186
Query: 177 LDEIRSLAEHGFKEIILSGIHTASYGVDLDEKVTLVDLLEEIEKIDGIERVRIGSIDPTF 236
+ E+ LA G+KE+ L G + SYG L EK+ DLL + GIER+R + P
Sbjct: 187 IRELEELAAAGYKEVTLLGQNVNSYGRGLGEKIEFADLLYRANSVAGIERIRFTTSHPKD 246
Query: 237 FTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSIT 296
++ +++ I +KLC H H LQ+G + L+RMNR Y+ + Y +V +R + VSIT
Sbjct: 247 VSDRLLQAIAECEKLCEHIHAPLQAGSNRILQRMNRNYSREHYLKLVERMRHYVPGVSIT 306
Query: 297 TDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSK 356
+D+IVGFPG ++R+ F YS R GTRA + Q+ +K +R +
Sbjct: 307 SDLIVGFPGETEEDFLETLDMVERVRFDAAFTFLYSQRSGTRAAELAEQIPLEEKKQRLE 366
Query: 357 KLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSGKIL 416
+L ++ G + +VL E + T TR+ V ++ G+++
Sbjct: 367 RLNRRQYQIATEINQELQGSIQEVLVEGPSKTNPQKLTSRTRSNRIVIFSGGKDLIGRLI 426
Query: 417 NVKINSFEGEVAEGEI 432
NVKI + GEI
Sbjct: 427 NVKITEAKTFSLFGEI 442
>MIAB_RICBR RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 446
Score = 216 bits (549), Expect = 4e-58
Identities = 142/444 (31%), Positives = 227/444 (51%), Gaps = 21/444 (4%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIG 61
K+ T GC++N Y+S M + GYE + E ADV ++NTC + +K+ +G
Sbjct: 4 KLYIKTYGCQMNVYDSVKMQDLLYPYGYEPTENIEEADVIILNTCHIREKAAEKTYSELG 63
Query: 62 RARRQNP--------EAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKAR 112
R ++ AII V GC AQ E+ + VD+V+G ++ + ++K
Sbjct: 64 RIKKLQDARKKQGLNSAIIVVAGCVAQAEGEEIFTRTPYVDIVVGPQSYYNLPELISKVV 123
Query: 113 DEGKQQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKD 172
K + + V + K F+ L + Y +F+ +Q+GC++FC +C++PYTRG+ S++
Sbjct: 124 RHEKHLIDLDFVEEAK-FDQLPEQLYPQGASSFISVQEGCDKFCTFCVVPYTRGAEFSRN 182
Query: 173 PKKVLDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKV-TLVDLLEEIEKIDGIERVRIG 230
++V E + G KEI+L G + +Y G D+KV TL DL+ + KI +ER+R
Sbjct: 183 VEQVYREALKIVSSGAKEIMLLGQNVNAYHGKTSDDKVFTLADLIRHLVKIPNLERLRYT 242
Query: 231 SIDPTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKI 290
+ P T+D++ KL P HL +QSG + TLK MNR++ Y DI++ LR
Sbjct: 243 TSHPIDMTDDLISLHGLEPKLMPFLHLPVQSGSNKTLKAMNRKHDRDYYFDIIDRLRKAR 302
Query: 291 EDVSITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNK 350
D+ +++D IVGFPG ++++ + + FKYSPR GT +QV +
Sbjct: 303 ADIVLSSDFIVGFPGETDEDFADTLDLVRKVKYGQCYSFKYSPRPGTPGATRTDQVPEHI 362
Query: 351 KDERSKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVY--TGYTRNYIKVNAKAD 408
K ER L + + AF E IG + VLF+ + + + T Y ++ N D
Sbjct: 363 KSERLTILQKELAAQQLAFNESCIGSTMKVLFDRDGKFDDQIIGKTPYMQSVYIKNPNKD 422
Query: 409 CNVSGKILNVKI-----NSFEGEV 427
+ GKI+ VKI NS GE+
Sbjct: 423 --LLGKIIEVKITKAALNSLSGEI 444
>MIAB_RICB8 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 446
Score = 215 bits (548), Expect = 5e-58
Identities = 142/444 (31%), Positives = 227/444 (51%), Gaps = 21/444 (4%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIG 61
K+ T GC++N Y+S M + GYE + E ADV ++NTC + +K+ +G
Sbjct: 4 KLYIKTYGCQMNVYDSVKMQDLLYPYGYEPTENIEEADVIILNTCHIREKAAEKTYSELG 63
Query: 62 RARRQNP--------EAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKAR 112
R ++ AII V GC AQ E+ + VD+V+G ++ + ++K
Sbjct: 64 RIKKLQDARKKQGLNSAIIVVAGCVAQAEGEEIFTRTPYVDIVVGPQSYYNLPELISKVV 123
Query: 113 DEGKQQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKD 172
K + + V + K F+ L + Y +F+ +Q+GC++FC +C++PYTRG+ S++
Sbjct: 124 RHEKHLIDLDFVEEAK-FDQLPEQLYPQGASSFISVQEGCDKFCTFCVVPYTRGAEFSRN 182
Query: 173 PKKVLDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKV-TLVDLLEEIEKIDGIERVRIG 230
++V E + G KEI+L G + +Y G D+KV TL DL+ + KI +ER+R
Sbjct: 183 VEQVYREALKVVSSGAKEIMLLGQNVNAYHGKTSDDKVFTLADLIRHLVKIPNLERLRYT 242
Query: 231 SIDPTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKI 290
+ P T+D++ KL P HL +QSG + TLK MNR++ Y DI++ LR
Sbjct: 243 TSHPIDMTDDLISLHGLEPKLMPFLHLPVQSGSNKTLKAMNRKHDRDYYFDIIDRLRKAR 302
Query: 291 EDVSITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNK 350
D+ +++D IVGFPG ++++ + + FKYSPR GT +QV +
Sbjct: 303 ADIVLSSDFIVGFPGETDEDFADTLDLVRKVKYGQCYSFKYSPRPGTPGATRTDQVPEHI 362
Query: 351 KDERSKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVY--TGYTRNYIKVNAKAD 408
K ER L + + AF E IG + VLF+ + + + T Y ++ N D
Sbjct: 363 KSERLTILQKELAAQQLAFNESCIGSTMKVLFDRDGKFDDQIIGKTPYMQSVYIKNPNKD 422
Query: 409 CNVSGKILNVKI-----NSFEGEV 427
+ GKI+ VKI NS GE+
Sbjct: 423 --LLGKIIEVKITKAALNSLSGEI 444
>MIAB_GEOUR RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 440
Score = 215 bits (548), Expect = 5e-58
Identities = 132/430 (30%), Positives = 217/430 (50%), Gaps = 11/430 (2%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIGR---A 63
T GC++N +SE + GY + AD+ ++NTCSV ++K + +
Sbjct: 11 TFGCQMNVSDSEKIAALLKGIGYFPTQDSSQADLVILNTCSVRAKAEEKVYNHLVQYKGL 70
Query: 64 RRQNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVKVG 122
+R+ P I+ V GC AQ + + + +D+V G+ N + V +A ++G++ +VG
Sbjct: 71 KRKKPGIILGVGGCVAQQEGERLLANVPHLDIVFGTHNLHLLPELV-RAAEKGERLAEVG 129
Query: 123 AVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDEIRS 182
+ + ++E + F+ + GC FC+YC++PY RG S+ +L E+R
Sbjct: 130 FIDNETRLDLFPVDERTDGVSRFVTVMQGCENFCSYCIVPYVRGREISRRSADILGEVRG 189
Query: 183 LAEHGFKEIILSGIHTASYGVDLDEKVTLVDLLEEIEKIDGIERVRIGSIDPTFFTEDVV 242
+A +G KE+ L G + SYG+ +++ + LL E+ I GIER+R + P F++ ++
Sbjct: 190 MAGNGVKEVTLLGQNVNSYGLKSSGEMSFIALLREVSLIPGIERIRFTTSHPKDFSQPLI 249
Query: 243 RRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITTDVIVG 302
+ KLC H HL QSG +A L MNR YT +EY + L+ + IT D+IVG
Sbjct: 250 DCFAEIPKLCRHIHLPAQSGSNAVLAAMNRGYTREEYLGSIARLKAACPSIQITGDIIVG 309
Query: 303 FPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSKKLIELN 362
FPG ++ + + F YS R T+A ++V ++K R +L++L
Sbjct: 310 FPGETEEDFQATLSLMEEVRYTDVFSFIYSKRPETKAAGYADEVGQDEKQGRLSRLLDLQ 369
Query: 363 RINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSGKILNVKI-- 420
R + ++G V VL E E G G + G T VN AD ++ G I+NV I
Sbjct: 370 RRITLETNKSFVGTVQQVLIEGESRRG-GQFYGRTSGNRVVNLAADVSLVGSIVNVMITR 428
Query: 421 ---NSFEGEV 427
NS +GE+
Sbjct: 429 GDQNSLQGEL 438
>MIAB_THEYD RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 431
Score = 215 bits (547), Expect = 7e-58
Identities = 139/441 (31%), Positives = 227/441 (51%), Gaps = 27/441 (6%)
Query: 3 VAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIGR 62
V T GC++N ++SE M +G+ VD + AD+ + NTC++ + ++K +GR
Sbjct: 6 VYIKTFGCQMNEHDSERMLGILGTKGFIEVDEPKKADIVIFNTCAIRHKAEQKFFSSLGR 65
Query: 63 AR---RQNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKA------R 112
+ ++NP+ I V GC AQ+ ++ + + +D ++G N I +
Sbjct: 66 VKHLKKKNPQLKIIVAGCSAQLQGEKLLNKLPYIDYIIGPDNLHVIENIIENQVSHRIFT 125
Query: 113 DEGKQQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKD 172
DE + + +K K + +A++ I GCN +C YC++PYTRG S+
Sbjct: 126 DENPEVANINLPVKRK-----------DCVKAWVNIIYGCNNYCTYCVVPYTRGKERSRP 174
Query: 173 PKKVLDEIRSLAEHGFKEIILSGIHTASYGVDLDEKVTLVDLLEEIEKIDGIERVRIGSI 232
++ EI LAE G+KE+ L G + SY D LLE++EKI+GI+R+R +
Sbjct: 175 VDDIIKEISLLAEQGYKEVTLLGQNVNSYK---DGNTNFPLLLEKVEKIEGIKRIRFITS 231
Query: 233 DPTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIED 292
P ++++V + KK+C H HL LQ+G + LK MNR+YT +EY + + LR+ I D
Sbjct: 232 HPKDLSKELVDVMKDYKKICEHIHLPLQAGSNKILKLMNRKYTYEEYFEKICWLREAIPD 291
Query: 293 VSITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKD 352
++IT+D+IVGFP LK I FK+SPR GT A ++ + K
Sbjct: 292 IAITSDIIVGFPQEQHEDFEKTINALKEIRFDGIFAFKFSPRLGTAAAKLDGHISEEVKA 351
Query: 353 ERSKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVS 412
R ++++L + ++ G++ +VL E + E G TG TR V +D +
Sbjct: 352 ARLIEVLKLQDEITERKNKRLEGKIQEVLVEGKDE--EGFTTGKTRTNKVVKIYSDIK-A 408
Query: 413 GKILNVKINSFEGEVAEGEIV 433
G+I+NVKI EG+I+
Sbjct: 409 GEIVNVKIAKTHRHSLEGDII 429
>MIAB_THEAB RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 429
Score = 215 bits (547), Expect = 7e-58
Identities = 134/398 (33%), Positives = 217/398 (54%), Gaps = 13/398 (3%)
Query: 1 MKVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
MK T GC++N +SE +EGYE + AD+ ++NTC V + K I
Sbjct: 1 MKFFIKTYGCQMNENDSEVARYYLEQEGYESAENENEADIVILNTCVVRKKAEDKFLSTI 60
Query: 61 GRARRQNPEAIIAVVGCYAQIAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVK 120
G R++N + I V+GC A+ ++ GV+ V+G+R I V E + K
Sbjct: 61 GELRKKNKK--IGVMGCGAEKLKEDLFK-RGVNFVIGTRAISRIPEAV-----ELSIKGK 112
Query: 121 VGAVLKNKVFE-DLK-IEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLD 178
A+ +K+ E D + I + N+K A++ I GCNRFC YC++PYTRG S+ +L
Sbjct: 113 KAAIFDDKLDEIDYRNILKRNSKHHAWITIIYGCNRFCTYCIVPYTRGREKSRKMDDILR 172
Query: 179 EIRSLAEHGFKEIILSGIHTASYGVDLDEKVTLVDLLEEIEKIDGIERVRIGSIDPTFFT 238
E+++L+ +G +EI G + +YG DL++ +L LL E +KI+ IER+ + PT F+
Sbjct: 173 EVKNLSLNGVREITYLGQNVDAYGKDLNDGTSLAKLLNETKKIENIERIWFLTSYPTDFS 232
Query: 239 EDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITTD 298
D+ R I + +K+ HL +Q G + LK+MNRRYT +EY +++ +R+ + D SI++D
Sbjct: 233 LDIAREIASSEKIAKSIHLPVQHGSNKILKKMNRRYTIEEYYELIKSIREIVPDASISSD 292
Query: 299 VIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRA-ENMENQVDGNKKDERSKK 357
+IVGFP ++ I + ++ YSPR+GT A + E+ V K R
Sbjct: 293 IIVGFPDETEDDFQQTVKLVEEIKFERLNLAIYSPREGTIAWKYFEDNVPRAIKTRRMAY 352
Query: 358 LIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTG 395
L+ L + K E Y+ + ++V+ EE + SG++ G
Sbjct: 353 LLNLQKEINKMLNESYLDKTVEVIVEERAK--SGLFYG 388
>MIAB_CAUSK RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 450
Score = 215 bits (547), Expect = 7e-58
Identities = 134/424 (31%), Positives = 219/424 (51%), Gaps = 13/424 (3%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKS-------RQI 59
T GC++N Y+SE M + GY +VD E AD+ V+NTC + +K +Q+
Sbjct: 13 TYGCQMNVYDSERMADVLRPLGYGIVDEPEGADLVVLNTCHIREKATEKVYSELGYIKQM 72
Query: 60 IGRARRQNPEAIIAVVGCYAQIAPTEVSGIE-GVDVVLGSRNKGEIVYYVNKARDEGKQQ 118
GR + +AV GC AQ E+ + VD+V+G + ++ + +A ++
Sbjct: 73 KGRKAEAGGQMTVAVAGCVAQAEGQEIMRRQPAVDLVVGPQAYHQLPELIARAHRATGER 132
Query: 119 VKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLD 178
+ K F+ L E + AFL +Q+GC++FC +C++PYTRG S+ P+++ D
Sbjct: 133 LSADFAADEK-FDALPAERQVSGVSAFLTVQEGCDKFCTFCVVPYTRGGEWSRPPEQIED 191
Query: 179 EIRSLAEHGFKEIILSGIHTASYGVDLDEKVTLVDLLEEIEKIDGIERVRIGSIDPTFFT 238
E R LA+ G +E+ L G + +Y TL L+ + KI G++R+R + P
Sbjct: 192 EARRLADQGVREVTLLGQNVNAYD---GGGYTLARLVRRLAKIPGLDRIRYTTSHPRDMG 248
Query: 239 EDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITTD 298
+D++ L +L P+ HL +Q+G D LK MNR +TA+ Y ++ +R D++++ D
Sbjct: 249 DDLIEAHGELPELMPYLHLPVQAGSDKILKAMNRDHTAESYIRLIEKIRQARPDIAMSGD 308
Query: 299 VIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSKKL 358
IVGFPG ++ + + FKYS R GT A M QVD K ER ++L
Sbjct: 309 FIVGFPGERDGDFEKTLDLVREVGFASAFSFKYSRRPGTPASAMPGQVDEAVKAERLERL 368
Query: 359 IELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSGKILNV 418
+L +KAF +G+ + VLFE++ G G + V+A ++ G+I+ V
Sbjct: 369 NQLLDEQQKAFNILQVGKTMPVLFEKKGR-NPGQIVGRSPYLQAVHAVGSEDLLGQIVPV 427
Query: 419 KINS 422
+I S
Sbjct: 428 RIES 431
>MIAB_ALKMQ RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 476
Score = 215 bits (547), Expect = 7e-58
Identities = 141/437 (32%), Positives = 231/437 (52%), Gaps = 16/437 (3%)
Query: 7 TLGCRVNTYESEAMTEKFVREGY-EVVDFNEMADVYVVNTCSVTNMGDKKSRQIIG---R 62
T GC++N ++SE + GY E D NE A++ + NTC V + K IG
Sbjct: 45 TYGCQMNEHDSEKLAGILQNIGYIETNDKNE-ANLIIYNTCCVRENAELKVYGNIGSLKN 103
Query: 63 ARRQNPEAIIAVVGCYAQIAPTEVSGIEG----VDVVLGSRNKGEIVYYVNKARDEGKQQ 118
+++ + IAV GC Q P V I+ VD+V G+ N + ++ + +
Sbjct: 104 LKKEKHDLTIAVCGCMMQ-QPHVVKEIKSKYRHVDLVFGTHNLHKFPELLSTSMESDNMI 162
Query: 119 VKVGAVLKNKVFEDLKI-EEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVL 177
+ V + + E L + +Y K AF+ I GCN FC YC++P+TRG S++ ++++
Sbjct: 163 IDVWDK-EGDIVEGLPVTRKYGLK--AFINIMFGCNNFCTYCIVPHTRGRERSREVQEII 219
Query: 178 DEIRSLAEHGFKEIILSGIHTASYGVDLDEKVTLVDLLEEIEKIDGIERVRIGSIDPTFF 237
DE+ LA++G KEI L G + SYG L+E+ +LL+ + KI+GIER+R + P
Sbjct: 220 DEVEELAKNGTKEITLLGQNVNSYGKTLEEETDFGNLLKVLNKIEGIERIRFMTSHPKDL 279
Query: 238 TEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITT 297
++ ++ + K+C H HL Q+G + LK MNR+YT + Y ++V +R K+ +V++TT
Sbjct: 280 SDSLISAMTVCTKVCAHLHLPFQAGSNDILKAMNRKYTKEAYLELVEKVRSKVPNVALTT 339
Query: 298 DVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSKK 357
D+IVGFPG +++ + + + YS R+ T A MEN V + K ER +
Sbjct: 340 DIIVGFPGETEDDFKETLNVVEKARFNSAYTYLYSLRQETPAAKMENHVPDDVKHERFNR 399
Query: 358 LIE-LNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSGKIL 416
LIE +NRI+ + + Y+ +V++VL E + G T VN + G+I+
Sbjct: 400 LIEAVNRISGE-INKAYLNKVVEVLVEGTSKTDDAKLMGRTPQSKLVNFAGNKEDIGQIV 458
Query: 417 NVKINSFEGEVAEGEIV 433
VKI + GE++
Sbjct: 459 RVKITEPKTFSLNGELL 475
>MIAB_MOOTA RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 444
Score = 214 bits (546), Expect = 9e-58
Identities = 124/361 (34%), Positives = 191/361 (52%), Gaps = 8/361 (2%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIGRARR- 65
T GC++N +SE M + GYE V E A V +++TC V + K +G+ +
Sbjct: 12 TYGCQMNQRDSEMMADLLQDAGYEPVAREEEAGVIILDTCCVREKAENKVYGKLGQIEKL 71
Query: 66 --QNPEAIIAVVGCYAQ---IAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVK 120
NP+ +IAV GC Q +A VD++LG+ N E+ + + + + ++
Sbjct: 72 KSANPDLVIAVAGCMVQQPGVAEKIRQQAPYVDLLLGTGNLQELPQLIEEIKAMHRPRIV 131
Query: 121 VGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDEI 180
VG + V EDL +AF+ I GCN FC YC++PY RG S+ P+ ++ E+
Sbjct: 132 VGEQ-EGPVVEDLPRRRARG-AQAFVTITYGCNNFCTYCIVPYVRGRERSRRPENIIKEV 189
Query: 181 RSLAEHGFKEIILSGIHTASYGVDLDEKVTLVDLLEEIEKIDGIERVRIGSIDPTFFTED 240
+ L + G E+ L G + SYG DL + + LLE + ++G++R+R + P FT +
Sbjct: 190 KELVDQGVIEVTLLGQNVNSYGRDLRDGINFAGLLERVNAVEGLKRIRYVTSHPRDFTPE 249
Query: 241 VVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITTDVI 300
+V I L K+C H HL +Q+G + L+ M+R YT + Y ++V LR I +S+TTD+I
Sbjct: 250 LVTTISRLDKVCEHVHLPVQAGSNRILELMHRGYTREHYLELVADLRRHIPGISLTTDLI 309
Query: 301 VGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSKKLIE 360
VGFPG + R+ F YSPR+GT A M Q+ K ER K+L+E
Sbjct: 310 VGFPGETEADFEDTLDLVARVQFDNAFTFMYSPRRGTEAATMPGQLPTAIKKERLKRLME 369
Query: 361 L 361
L
Sbjct: 370 L 370
>MIAB_CYAP8 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 451
Score = 214 bits (546), Expect = 9e-58
Identities = 133/439 (30%), Positives = 220/439 (50%), Gaps = 16/439 (3%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIGRA--- 63
T GC++N +SE M G+ + AD+ + NTC++ + ++K +GR
Sbjct: 17 TFGCQMNKADSERMAGILENMGFIWSEDPNQADLILYNTCTIRDNAEQKVYSYLGRQAKR 76
Query: 64 RRQNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVKVG 122
+ +NP+ + V GC AQ ++ + +D+V+G ++ + + + D G Q V
Sbjct: 77 KHENPDLTLIVAGCVAQQEGEQILRRVPELDLVMGPQHANRLEDLLQQVFD-GNQVVATE 135
Query: 123 AVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDEIRS 182
+ + ED+ ++ A++ + GCN C+YC++P RG S+ P+ +L E+
Sbjct: 136 PI---HIIEDITKPRRDSTITAWVNVIYGCNERCSYCVVPNVRGVEQSRTPEAILAEMEL 192
Query: 183 LAEHGFKEIILSGIHTASYGVDLD-------EKVTLVDLLEEIEKIDGIERVRIGSIDPT 235
L + G+KE+ L G + +YG DL + TL DLL + + GIER+R + P
Sbjct: 193 LGKQGYKEVTLLGQNIDAYGRDLPGVTESGRHQHTLTDLLYTVHDVVGIERIRFATSHPR 252
Query: 236 FFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSI 295
+FTE ++ L K+C HFH+ QSG + LK M R YT ++Y I++ +RD + D SI
Sbjct: 253 YFTERLIAACQELSKVCEHFHIPFQSGDNDILKAMKRGYTHEKYRQIIDKIRDYMPDASI 312
Query: 296 TTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERS 355
+ D IVGFPG ++ I + + YSPR GT A +NQ+ K +R
Sbjct: 313 SADAIVGFPGETEEQFENTLKLVEDIGFDQLNTAAYSPRPGTPAALWDNQLSEEVKSDRL 372
Query: 356 KKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCN-VSGK 414
++L L +++Y+G + +VL E++ S G TR K D N + G+
Sbjct: 373 QRLNHLVAQKAAERSQRYLGRIEEVLVEDQNPKDSTQVMGRTRGNRLTFFKGDINQLKGR 432
Query: 415 ILNVKINSFEGEVAEGEIV 433
++ VKI GEIV
Sbjct: 433 LIKVKITEVRAFSLTGEIV 451
>MIAB_SULSY RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 437
Score = 214 bits (545), Expect = 1e-57
Identities = 138/441 (31%), Positives = 217/441 (49%), Gaps = 15/441 (3%)
Query: 1 MKVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
MK T GC++N +SE M GY + E ADV +VNTCSV D+K +
Sbjct: 1 MKFYIKTFGCQMNVNDSEKMAGILQTLGYTPTENWEEADVILVNTCSVREKPDQKVLSAL 60
Query: 61 G---RARRQNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
G + ++ NP A+I V GC AQ A E+ +D+V G+ N ++++ +E K
Sbjct: 61 GEFKKVKKHNPNAVIGVCGCLAQRAGYEIYQKAPFIDIVFGTTN----IHHLPNLLEEAK 116
Query: 117 QQVKVGAVLKNKVFEDLKIEEY----NNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKD 172
K ++ NK AF+ + GC++ C YC++P TRG S+
Sbjct: 117 SGNKAXXXXXXXXXXXXXXXQFPTVRENKYTAFVTVIRGCDKKCTYCIVPTTRGRERSRR 176
Query: 173 PKKVLDEIRSLAEHGFKEIILSGIHTASYGVDLDEKVTLVDLLEEIEKIDGIERVRIGSI 232
+L E++ L E G KEI L G + +YG D + V +LL+ + ++DG+ER+R +
Sbjct: 177 IGDILREVQYLVEDGVKEIHLIGQNVTAYGKDFGD-VKFWELLKAVAEVDGVERIRFTTG 235
Query: 233 DPTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIED 292
P ED ++ + L ++C HL +Q+G D L+ M+R YT KEY + LL+ I +
Sbjct: 236 HPRDLDEDTIKVMADLPQICEALHLPIQAGSDRILQAMDRGYTQKEYLQKIELLKKYIPN 295
Query: 293 VSITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKD 352
++++TD+IVGFPG +K + + FKYSPR GT A ++ +
Sbjct: 296 IALSTDIIVGFPGETYEDYLETVKVIKEVEYDQVFAFKYSPRPGTPAADLPMTESPEELS 355
Query: 353 ERSKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVS 412
+R LI L + +Y +++++L EE + G TR V A+
Sbjct: 356 KRLNDLINLQKDITFKKNLEYQDKIVEILVEEINQ--ENKLVGRTRTNKLVYAEGSPEYL 413
Query: 413 GKILNVKINSFEGEVAEGEIV 433
GK++NVKI EG I+
Sbjct: 414 GKLVNVKIEKVNRFSLEGSII 434
>MIAB_THESQ RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 443
Score = 214 bits (544), Expect = 1e-57
Identities = 136/444 (30%), Positives = 237/444 (53%), Gaps = 25/444 (5%)
Query: 1 MKVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
M+ T GC++N +SE M ++EG+ E ADV ++NTC+V ++K+ +
Sbjct: 1 MRFYIKTFGCQMNENDSETMAGLLMKEGFTPASAPEEADVVIINTCAVRRKSEEKAYSEL 60
Query: 61 GRARR--QNPEAIIAVVGCYAQIAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQ 118
G+ + + + ++ V GC A+ ++ G D VLG+R V V +A Q
Sbjct: 61 GQMLKIKRKRKLVVGVAGCVAEKEREKLLE-RGADFVLGTR----AVLKVTEAVKRALQG 115
Query: 119 VKVGAVLKNKVFEDLKIEEY--------NNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCS 170
KV +FED ++EY ++K A++ I GC+RFC YC++PYTRG S
Sbjct: 116 EKVA------LFED-HLDEYTHELPRIRSSKHHAWVTIIFGCDRFCTYCIVPYTRGREKS 168
Query: 171 KDPKKVLDEIRSLAEHGFKEIILSGIHTASYGVDLDEKVTLVDLLEEIEKIDGIERVRIG 230
+ + +L+E+R LA+ G++E+ G + +YG DL + +L LLEE KI+GIER+
Sbjct: 169 RPMEDILEEVRELAKQGYREVTFLGQNVDAYGKDLKDGSSLAKLLEEASKIEGIERIWFL 228
Query: 231 SIDPTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKI 290
+ PT F+++++ I K+ HL +QSG + LK MNR YT +EY ++ +R K+
Sbjct: 229 TSYPTDFSDELIEVIARNPKVAKSVHLPVQSGSNRILKLMNRSYTKEEYLALLERIRSKV 288
Query: 291 EDVSITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRA-ENMENQVDGN 349
DV+I++D+IVGFP +++ + ++ YSPRKGT A ++ +++V
Sbjct: 289 PDVAISSDIIVGFPTETEEDFMETIDLVEKAQFERLNLAIYSPRKGTVAWKHYKDEVPYE 348
Query: 350 KKDERSKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADC 409
+K R + L+ L + + E+Y G+ + V+ E + + +G++ G + + +
Sbjct: 349 EKVRRMQFLMNLQKRINRKLNERYKGKTVRVIVEAQAK--NGLFYGRDIRNKIIAFEGEE 406
Query: 410 NVSGKILNVKINSFEGEVAEGEIV 433
+ G+ +VKI G++V
Sbjct: 407 WMIGRFADVKIEKITAGPLYGKVV 430
>MIAB_THEP1 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 443
Score = 214 bits (544), Expect = 1e-57
Identities = 136/444 (30%), Positives = 237/444 (53%), Gaps = 25/444 (5%)
Query: 1 MKVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
M+ T GC++N +SE M ++EG+ E ADV ++NTC+V ++K+ +
Sbjct: 1 MRFYIKTFGCQMNENDSETMAGLLMKEGFTPASAPEEADVVIINTCAVRRKSEEKAYSEL 60
Query: 61 GRARR--QNPEAIIAVVGCYAQIAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQ 118
G+ + + + ++ V GC A+ ++ G D VLG+R V V +A Q
Sbjct: 61 GQMLKIKRKRKLVVGVAGCVAEKEREKLLE-RGADFVLGTR----AVLKVTEAVKRALQG 115
Query: 119 VKVGAVLKNKVFEDLKIEEY--------NNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCS 170
KV +FED ++EY ++K A++ I GC+RFC YC++PYTRG S
Sbjct: 116 EKVA------LFED-HLDEYTHELPRIRSSKHHAWVTIIFGCDRFCTYCIVPYTRGREKS 168
Query: 171 KDPKKVLDEIRSLAEHGFKEIILSGIHTASYGVDLDEKVTLVDLLEEIEKIDGIERVRIG 230
+ + +L+E+R LA+ G++E+ G + +YG DL + +L LLEE KI+GIER+
Sbjct: 169 RPMEDILEEVRELAKQGYREVTFLGQNVDAYGKDLKDGSSLAKLLEEASKIEGIERIWFL 228
Query: 231 SIDPTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKI 290
+ PT F+++++ I K+ HL +QSG + LK MNR YT +EY ++ +R K+
Sbjct: 229 TSYPTDFSDELIEVIARNPKVAKSVHLPVQSGSNRILKLMNRSYTKEEYLALLERIRSKV 288
Query: 291 EDVSITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRA-ENMENQVDGN 349
DV+I++D+IVGFP +++ + ++ YSPRKGT A ++ +++V
Sbjct: 289 PDVAISSDIIVGFPTETEEDFMETIDLVEKAQFERLNLAIYSPRKGTVAWKHYKDEVPYE 348
Query: 350 KKDERSKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADC 409
+K R + L+ L + + E+Y G+ + V+ E + + +G++ G + + +
Sbjct: 349 EKVRRMQFLMNLQKRINRKLNERYKGKTVRVIVEAQAK--NGLFYGRDIRNKIIAFEGEE 406
Query: 410 NVSGKILNVKINSFEGEVAEGEIV 433
+ G+ +VKI G++V
Sbjct: 407 WMIGRFADVKIEKITAGPLYGKVV 430
>MIAB_PARUW RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 450
Score = 214 bits (544), Expect = 1e-57
Identities = 138/429 (32%), Positives = 227/429 (52%), Gaps = 5/429 (1%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIGR-ARR 65
T GC++N +SE M + G AD+ + NTCS+ ++ ++K +G+
Sbjct: 23 TYGCQMNELDSEIMIGQLENRGLTRSHDENDADLLIFNTCSIRDLAERKVMGKLGKLGLT 82
Query: 66 QNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVKVGAV 124
+ +AII V GC A + + +D VLG+ N ++ + +++ GKQ ++
Sbjct: 83 KQSQAIIGVTGCMANAKKDSLFQKLPHIDFVLGTNNIHDLNHVLDEVLASGKQSIRTDDH 142
Query: 125 LKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDEIRSLA 184
+ ++ D + ++ +A++ I GC++FC YC++PYTRGS S+ P+ +L+E R L
Sbjct: 143 FEFEL--DYLNAKREDQIKAYVSIIRGCDKFCTYCVVPYTRGSEVSRAPENILEECRHLV 200
Query: 185 EHGFKEIILSGIHTASYGVD-LDEKVTLVDLLEEIEKIDGIERVRIGSIDPTFFTEDVVR 243
G+KEI L G + SYG D L+ K DLL +++KI G+ERVR + P +++++
Sbjct: 201 NQGYKEITLLGQNVNSYGKDKLEWKCLFHDLLYQLDKIPGLERVRFMTSHPVDISKELME 260
Query: 244 RILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITTDVIVGF 303
I LK LC H LQ+G + LK+M+R YT ++Y + V +L++ + +V++ TD+IVGF
Sbjct: 261 AIRDLKTLCEFVHFPLQAGSNRVLKKMHRIYTVEQYLEKVQMLKEIVPNVALGTDIIVGF 320
Query: 304 PGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSKKLIELNR 363
P LK I S +F YSPRKGT A + V K
Sbjct: 321 PXXXXXXXXXXYRLLKEIEYSVAFLFSYSPRKGTPAMRWRDDVPEEVKXXXXXXXXXXXX 380
Query: 364 INEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSGKILNVKINSF 423
+ ++G+ ++VL E + G TR + V + G + VKI+ +
Sbjct: 381 TIYMKHRQAFLGQTVEVLVERRNFKDDRLVKGRTRCWKNVLFTGGDELVGTMQQVKIHGY 440
Query: 424 EGEVAEGEI 432
+ G++
Sbjct: 441 SHQTLLGDL 449
>MIAB_HYDS0 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 435
Score = 213 bits (543), Expect = 2e-57
Identities = 129/428 (30%), Positives = 221/428 (51%), Gaps = 17/428 (3%)
Query: 1 MKVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
MK T GC++N +SE + +GYE E AD+ ++NTC++ D+K +
Sbjct: 1 MKYFIKTYGCQMNINDSEKIKGILQTQGYEPATREEDADLVILNTCTIREKPDQKVWSHL 60
Query: 61 GRARRQ---NPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
G ++ NP I V GC AQ A E+ S + +D+V G++N ++++ K ++ K
Sbjct: 61 GEIKKLKSINPNIKIGVCGCMAQRAGYEIASKMPFIDLVFGTKN----IHHMPKLLEDVK 116
Query: 117 QQVKVGAVLKNKVFEDLKIEEY----NNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKD 172
+ +L+ + + ++ Y +N A++ I GC++ C YC++P+TRG S++
Sbjct: 117 VGNRAIEILEEEDPSENILDSYPTVRDNSYCAYVTIMRGCDKECTYCVVPFTRGKERSRN 176
Query: 173 PKKVLDEIRSLAEHGFKEIILSGIHTASYGVDLDEKVTLVDLLEEIEKIDGIERVRIGSI 232
P+ +LDE++SL + G EI L G + +YG D+D LLE I KI+G++R+R +
Sbjct: 177 PQSILDEVKSLVDSGVMEIHLIGQNVTAYGKDID--YPFYKLLENISKIEGVKRIRFTTG 234
Query: 233 DPTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIED 292
P T+D++ + L + H HL Q+G D L+ M R YT Y + + L+ KI+D
Sbjct: 235 HPIDMTDDIIETMANLPNMVNHLHLPFQAGSDRILELMKRNYTKAYYLNRIEKLKAKIKD 294
Query: 293 VSITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKD 352
++ +TD+I+GFP ++ + + FKYS R T A ME+ + K
Sbjct: 295 ITFSTDIIIGFPTETEEDFQHTLDVVQTVEFEQVFSFKYSKRPNTPAYYMEDDLTDEIKT 354
Query: 353 ERSKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVS 412
+R K+L+E+ + ++Y V VL E++ Y G T + N + ++
Sbjct: 355 DRIKRLLEIQKAITSKLMQRYKNTVQKVLVEDK---KGNTYIGRTTTNVWCNITSSQDIL 411
Query: 413 GKILNVKI 420
GK + V I
Sbjct: 412 GKEVEVLI 419
>MIAB_THEMA RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 443
Score = 213 bits (541), Expect = 3e-57
Identities = 139/444 (31%), Positives = 231/444 (52%), Gaps = 25/444 (5%)
Query: 1 MKVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
M+ T GC++N +SEAM V+EG+ E ADV ++NTC+V ++K+ +
Sbjct: 1 MRFYIKTFGCQMNENDSEAMAGLLVKEGFTPASSPEEADVVIINTCAVRRKSEEKAYSEL 60
Query: 61 GRARRQNPEA--IIAVVGCYAQIAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQ 118
G+ ++ V GC A+ E +G D VLG+R + V KA EG+
Sbjct: 61 GQVXXXXXXXXIVVGVAGCVAE-KEREKFLEKGADFVLGTRAVPRVTEAVKKAL-EGE-- 116
Query: 119 VKVGAVLKNKVFEDLKIEEYNNKT--------RAFLKIQDGCNRFCAYCLIPYTRGSVCS 170
K +FED ++EY ++ A++ I GC+RFC YC++PYTRG S
Sbjct: 117 -------KVALFED-HLDEYTHELPRIRTSRHHAWVTIIHGCDRFCTYCIVPYTRGRERS 168
Query: 171 KDPKKVLDEIRSLAEHGFKEIILSGIHTASYGVDLDEKVTLVDLLEEIEKIDGIERVRIG 230
+ +L+E++ LAE G++E+ G + +YG DL + +L LLEE KI+GIER+
Sbjct: 169 RPMADILEEVKKLAEQGYREVTFLGQNVDAYGKDLKDGSSLAKLLEEASKIEGIERIWFL 228
Query: 231 SIDPTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKI 290
+ PT F+++++ I K+ HL +QSG + LK MNRRYT +EY ++ +R K+
Sbjct: 229 TSYPTDFSDELIEVIAKNPKVAKSVHLPVQSGSNRILKLMNRRYTKEEYLALLEKIRSKV 288
Query: 291 EDVSITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRA-ENMENQVDGN 349
+V+I++D+IVGFP +++ + ++ YSPR+GT A + ++ V
Sbjct: 289 PEVAISSDIIVGFPTETEEDFMETVDLVEKAQFERLNLAIYSPREGTVAWKYYKDDVPYE 348
Query: 350 KKDERSKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADC 409
+K R + L+ L + + E+Y G+ + ++ E + + G Y RN I D
Sbjct: 349 EKVRRMQFLMNLQKRINRKLNERYRGKTVRIIVEAQAKNGL-FYGRDIRNKIIAFEGEDW 407
Query: 410 NVSGKILNVKINSFEGEVAEGEIV 433
+ G+ +VK+ G++V
Sbjct: 408 MI-GRFADVKVEKITAGPLYGKVV 430
>MIAB_RHIL3 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 473
Score = 213 bits (541), Expect = 3e-57
Identities = 141/450 (31%), Positives = 228/450 (50%), Gaps = 22/450 (4%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIG 61
KV T GC++N Y+S M++ R+GYE + E+AD+ ++NTC + +K +G
Sbjct: 27 KVFIKTYGCQMNVYDSMRMSDALARDGYEPTEDMEVADLVLLNTCHIREKAAEKVYSALG 86
Query: 62 RARRQNP-------EAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARD 113
R R E +I V GC AQ E+ VDVV+G + + + +A+
Sbjct: 87 RLREMKKKKAADGREMMIGVAGCVAQAEGEEILRRAPAVDVVIGPQTYHRLPEALRRAK- 145
Query: 114 EGKQQVKVGAVLKNKVFEDLKIEEYNN----KTRAFLKIQDGCNRFCAYCLIPYTRGSVC 169
+G++ V +++K FE L I E AFL +Q+GC++FC +C++PYTRGS
Sbjct: 146 QGQRVVDTEYAIEDK-FEHLPIAESRRIRARGVTAFLTVQEGCDKFCTFCVVPYTRGSEV 204
Query: 170 SKDPKKVLDEIRSLAEHGFKEIILSGIHTASY--GVDLDEKVTLVDLLEEIEKIDGIERV 227
S+ ++++E LA+ G +EI L G + ++ E +L DLL + +I G+ R+
Sbjct: 205 SRSVSQIVEEAEKLADSGVREITLLGQNVNAWHGAGSQGEAWSLGDLLYRLAEIPGLARL 264
Query: 228 RIGSIDPTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLR 287
R + P + ++ L L P+ HL +QSG D LK MNRR+TA EY ++ +R
Sbjct: 265 RYTTSHPRDMDDRLINAHRDLSALMPYLHLPVQSGSDRILKAMNRRHTAAEYLALIERIR 324
Query: 288 DKIEDVSITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVD 347
D++++ D I GFPG ++ + ++ FKYS R GT +++QV
Sbjct: 325 TVRPDIALSGDFITGFPGETDEDFKDTLRLVEEVRYAQAFSFKYSTRPGTPGAELKDQVP 384
Query: 348 GNKKDERSKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKA 407
K ER ++L L ++ FAE +G+ ID+L E+ + + G + VN A
Sbjct: 385 EEIKAERLERLQMLLLKQQQEFAESCVGKEIDLLLEKPGRMPEQL-IGRSPWLQSVNVDA 443
Query: 408 DCNVSGKILNVKI-----NSFEGEVAEGEI 432
+ G I+ V+I NS E AE +
Sbjct: 444 KASQIGDIIKVRITGTGTNSLFAEHAEAAV 473
>MIAB_GEOTN RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 523
Score = 213 bits (541), Expect = 3e-57
Identities = 135/439 (30%), Positives = 226/439 (51%), Gaps = 12/439 (2%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIG 61
K T GC++N +++E M F+ GYE D E A+V ++NTC++ + K +G
Sbjct: 81 KFYIRTYGCQMNEHDTEVMAGIFMALGYEPTDRPEDANVILLNTCAIRENAENKVFGELG 140
Query: 62 RAR---RQNPEAIIAVVGCYAQ---IAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEG 115
+ NP+ ++ V GC +Q + + + VD++ G+ N + Y +++A
Sbjct: 141 YLKPLKTTNPDLLLGVCGCMSQEEAVVNKILKQYQYVDLIFGTHNIHRLPYILHEAYMSK 200
Query: 116 KQQVKVGAVLKNKVFEDL-KIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPK 174
+ V+V + + V E+L K+ + N K A++ I GC++FC YC++PYTRG S+ P+
Sbjct: 201 EMVVEVWSK-EGDVVENLPKVRKGNIK--AWVNIMYGCDKFCTYCIVPYTRGKERSRRPE 257
Query: 175 KVLDEIRSLAEHGFKEIILSGIHTASYGVDLDE-KVTLVDLLEEIEKIDGIERVRIGSID 233
++ E+R LA G+KEI L G + +YG D ++ + L DL++E+ KID I R+R +
Sbjct: 258 DIIQEVRQLAAQGYKEITLLGQNVNAYGKDFNDIQYGLGDLMDELRKID-IARIRFTTSH 316
Query: 234 PTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDV 293
P F + ++ + L H HL +QSG LK M R+YT +EY ++V ++ I DV
Sbjct: 317 PRDFDDRLIEVLAKRGNLVEHIHLPVQSGSTEILKMMGRKYTREEYLELVRKIKAAIPDV 376
Query: 294 SITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDE 353
++TTD+IVGFP + + + F YSPR+GT A M + V K E
Sbjct: 377 ALTTDIIVGFPNETDEQFEETLSLYREVEFDSAYTFIYSPREGTPAAKMNDNVPMEVKKE 436
Query: 354 RSKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSG 413
R ++L L + ++Y + V GYTR V+ ++ G
Sbjct: 437 RLQRLNALVQEIAAKKMKQYXXXXXXXXXXXXSKTNPDVLAGYTRKNKLVHFVGPKSLIG 496
Query: 414 KILNVKINSFEGEVAEGEI 432
+++NV+I + GE+
Sbjct: 497 QLVNVRITQAKTWTLTGEL 515
>MIAB_BRASO RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 467
Score = 213 bits (541), Expect = 3e-57
Identities = 139/446 (31%), Positives = 229/446 (51%), Gaps = 17/446 (3%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVREGY-EVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ + GC++N Y+++ M + EG+ E D + AD+ ++NTC + +K +
Sbjct: 6 KLHIKSYGCQMNVYDAQRMVDTLGAEGFVETADAGD-ADLVILNTCHIREKASEKVYSEL 64
Query: 61 GRARRQNPEAI-------IAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKAR 112
GR R EA IAV GC AQ E+ S VDVV+G ++ + + +A
Sbjct: 65 GRLRVAKEEAARSGRAMQIAVAGCVAQAEGVEIISRAPTVDVVVGPQSYHHLPQLLAQA- 123
Query: 113 DEGKQQVKVGAVLKNKVFEDLKIEEYNNKTR---AFLKIQDGCNRFCAYCLIPYTRGSVC 169
G++ ++ ++K K + R AF+ +Q+GC++FC +C++PYTRG+
Sbjct: 124 SRGERAIETEFPAEDKFGFLAKPSREAIRARGVSAFVTVQEGCDKFCTFCVVPYTRGAEM 183
Query: 170 SKDPKKVLDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKV-TLVDLLEEIEKIDGIERV 227
S+ +++D++ L + G +EI L G + +Y G D + TL LL I +I G+ R+
Sbjct: 184 SRPVARIVDDVMQLTDSGVREITLIGQNVNAYHGEGPDGRTWTLGRLLYRIAEIPGVARI 243
Query: 228 RIGSIDPTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLR 287
R + P + ++ L + P HL +QSG D L MNR+++A +Y +V+ R
Sbjct: 244 RYSTSHPNDVDDGLIAAHRDLTAVMPFVHLPVQSGSDRILAAMNRKHSAADYRRVVDRFR 303
Query: 288 DKIEDVSITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVD 347
+D++ ++D IVGFPG + +I + + FKYSPR GT A +M+ V
Sbjct: 304 GARDDIAFSSDFIVGFPGETEEDFRATLALIDQIGYAAAYSFKYSPRPGTPAADMQEMVS 363
Query: 348 GNKKDERSKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKA 407
+ DER ++L L + AF + IG V+DVLFE E G G T + A
Sbjct: 364 ATEMDERLERLQSLIDSQQAAFNKAAIGSVVDVLFEREAR-KPGQLVGRTAYLQPAHVMA 422
Query: 408 DCNVSGKILNVKINSFEGEVAEGEIV 433
++ G++L V+I+S E GE+V
Sbjct: 423 SSDIIGQVLPVRIDSLERYSLLGELV 448
>MIAB_ANOFW RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 527
Score = 213 bits (541), Expect = 3e-57
Identities = 140/441 (31%), Positives = 225/441 (51%), Gaps = 14/441 (3%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIG 61
K T GC++N +++E M F+ GYE D E A+V ++NTC++ + K IG
Sbjct: 85 KFYIRTYGCQMNEHDTEVMAGIFMALGYEPTDRPEDANVILLNTCAIRENAENKVFGEIG 144
Query: 62 RAR---RQNPEAIIAVVGCYAQ---IAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEG 115
+ + NP+ ++ V GC +Q + + + VD++ G+ N + Y + +A
Sbjct: 145 HLKPLKQNNPDLLLGVCGCMSQEESVVNKILKQFQYVDMIFGTHNIHRLPYILKEAYMSK 204
Query: 116 KQQVKVGAVLKNKVFEDL-KIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPK 174
+ V+V + + V E+L K+ + N K A++ I GC++FC YC++PYTRG S+ P+
Sbjct: 205 EMVVEVWSK-EGDVIENLPKVRKGNIK--AWVNIMYGCDKFCTYCIVPYTRGKERSRRPE 261
Query: 175 KVLDEIRSLAEHGFKEIILSGIHTASYGVDL-DEKVTLVDLLEEIEKIDGIERVRIGSID 233
++ E+R LA G+KEI L G + +YG D D K L DL++E+ KID I R+R +
Sbjct: 262 DIIQEVRHLAAQGYKEITLLGQNVNAYGKDFTDMKYGLGDLMDELRKID-IPRIRFTTSH 320
Query: 234 PTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDV 293
P F + ++ + L H HL +QSG LK M R+YT ++Y ++V +++ I V
Sbjct: 321 PRDFDDRLIEVLAKGGNLVEHIHLPVQSGSSEVLKMMARKYTREQYLELVRKIKEAIPGV 380
Query: 294 SITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDE 353
++TTD+IVGFP + + F YSPR+GT A M + V K E
Sbjct: 381 ALTTDIIVGFPNETDEQFEETLSLYREVEFDSAFTFIYSPREGTPAAKMVDNVPMEVKKE 440
Query: 354 RSKKLIEL-NRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVS 412
R ++L L N I+ K + + V GYTR VN +
Sbjct: 441 RLQRLNALVNEISAKKM-KXXXXXXXXXXXXXXSKNNPDVLAGYTRKNKLVNFVGPKSAI 499
Query: 413 GKILNVKINSFEGEVAEGEIV 433
G+++NV+I + GE+V
Sbjct: 500 GQLVNVRITEAKTWTLNGEMV 520
>MIAB_EHRRW RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 445
Score = 212 bits (540), Expect = 4e-57
Identities = 128/380 (33%), Positives = 202/380 (53%), Gaps = 7/380 (1%)
Query: 9 GCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIGRARR--Q 66
GC++N Y+S + G+ +V+ AD+ ++NTC + +K +GR R+ +
Sbjct: 10 GCQMNVYDSLIIENIIKPLGFSIVNEPSEADIVILNTCHIREKAAEKLYSELGRIRKIQE 69
Query: 67 NPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVKVGAVL 125
I V GC AQ TE+ + VD+V+G ++ + + KAR KQ + + +
Sbjct: 70 TKNLTIVVAGCVAQAEGTEIFTRAPFVDIVVGPQSIHTLPELIVKARKIKKQIINIDFPV 129
Query: 126 KNKVFEDLKIEEY--NNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDEIRSL 183
+K F+ + +EEY N K AF+ +Q+GCN+FC++C++PYTRG S+ + + E L
Sbjct: 130 ISK-FDAIAVEEYTKNQKVSAFISVQEGCNKFCSFCVVPYTRGEEYSRTVEAIFKEALIL 188
Query: 184 AEHGFKEIILSGIHTASY-GVDLDEKVTLVDLLEEIEKIDGIERVRIGSIDPTFFTEDVV 242
A+ G KEI L G + +Y G + L L++ I KI IER+ + P E +
Sbjct: 189 ADSGIKEITLIGQNVNAYHGTYKGNEWDLGRLIQHIAKISSIERIYYTTSHPRDMHESLY 248
Query: 243 RRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITTDVIVG 302
KKL P HL +QSG + L++MNR++TA+EY +I+ LR D++ ++D IVG
Sbjct: 249 EAHGIEKKLIPFIHLPVQSGSNKILRKMNRKHTAEEYINIIKTLRKHRSDIAYSSDFIVG 308
Query: 303 FPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSKKLIELN 362
FPG ++ + S+ + FKYSPR GT + +Q+ K +R KL EL
Sbjct: 309 FPGETDEDFENTIQLIEEVKFSQAYSFKYSPRPGTPSAEYTSQIPDEIKSQRLTKLQELV 368
Query: 363 RINEKAFAEKYIGEVIDVLF 382
+ F +K IGE VLF
Sbjct: 369 HKQQLEFNKKMIGETHPVLF 388
>MIAB_DESHY RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 447
Score = 212 bits (540), Expect = 4e-57
Identities = 134/437 (30%), Positives = 210/437 (48%), Gaps = 11/437 (2%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIG 61
KV GC+++ +++ +TE ++GY E AD+ +VNTC V + K IG
Sbjct: 9 KVVTLAYGCQMSERDADTLTEISSQKGYVRSQELEQADLIIVNTCCVRESAENKILGKIG 68
Query: 62 RARR---QNPEAIIAVVGCYAQIAPTEVSGIEG----VDVVLGSRNKGEIVYYVNKARDE 114
+ NP+ IA+ GC Q P + + VD+ G+ N E +
Sbjct: 69 ELKHLKEANPQLKIAISGCMVQ-QPGALERLRKRAPHVDIWAGTHNIHE---FQRLLXXX 124
Query: 115 GKQQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPK 174
+ ++ + K +A++ I GCN FC YC++P+ RG S+ P+
Sbjct: 125 XXXXXXXXXXXXPRETQESVLLAAKGKLKAYVNISYGCNNFCTYCIVPHVRGRERSRQPE 184
Query: 175 KVLDEIRSLAEHGFKEIILSGIHTASYGVDLDEKVTLVDLLEEIEKIDGIERVRIGSIDP 234
++L EIR+L E G +E+ L G + SYG DLD DLL++++ IDG+ RVR + P
Sbjct: 185 EILAEIRALVETGCREVTLLGQNVNSYGQDLDRAYDFADLLKDVDSIDGLWRVRFMTSHP 244
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
++ ++ I A LC H HL Q+G D LK MNR+YT + Y + ++ I VS
Sbjct: 245 KDLSDKLIETIAAGTHLCEHIHLPFQAGSDEILKGMNRKYTREYYLSRIAQIKVIIPQVS 304
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
+TTD+IVGFPG ++++ S+ F YS R GT A M Q+ + K R
Sbjct: 305 LTTDIIVGFPGETEEDFEQTLELIRQVRYSQAFTFMYSKRSGTPAAQMAEQIPLDIKKRR 364
Query: 355 SKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSGK 414
++LI + A+ ++ IG+ +VL E + TG TR Y V + + G
Sbjct: 365 LQQLITVQNAQSLAWRQEMIGKTCEVLVEGPSKSNPDRLTGRTRGYELVVFPGEAQLIGT 424
Query: 415 ILNVKINSFEGEVAEGE 431
++ V I GE
Sbjct: 425 LVQVLIQDANSWTLFGE 441
>MIAB_STAHJ RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 514
Score = 212 bits (539), Expect = 6e-57
Identities = 135/424 (31%), Positives = 219/424 (51%), Gaps = 14/424 (3%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIGRAR-- 64
T GC++N +++E M GY + ADV ++NTC++ + K IG +
Sbjct: 74 TYGCQMNAHDTEVMAGILQALGYTATEDINEADVILINTCAIRENAENKVFSEIGNLKHL 133
Query: 65 -RQNPEAIIAVVGCYAQ---IAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVK 120
+ PEA+I V GC +Q + + + VD++ G+ N ++ + +A V+
Sbjct: 134 KKNRPEALIGVCGCMSQEESVVNKILKSYQNVDMIFGTHNIHKLPEILEEAYLSKAMVVE 193
Query: 121 VGAVLKNKVFEDL-KIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDE 179
V + + + E+L K+ E T+A++ I GC++FC YC++P+TRG S+ P+ +++E
Sbjct: 194 VWSK-EGDIIENLPKVRE--GSTKAWVNIMYGCDKFCTYCIVPFTRGKERSRRPEDIIEE 250
Query: 180 IRSLAEHGFKEIILSGIHTASYGVDLDEKVTLVDLLEE-IEKIDGIERVRIGSIDPTFFT 238
+R LA G+KEI L G + SYG D+ I KID I RVR + P FT
Sbjct: 251 VRGLARDGYKEITLLGQNVNSYGKDIKXXXXXXXXXXXXISKID-IPRVRFTTSHPWDFT 309
Query: 239 EDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITTD 298
+ ++ I + PH HL +QSG +A LK M R+YT + Y D+VN ++ I +V++TTD
Sbjct: 310 DRMIEVIAKGGNIVPHIHLPVQSGNNAVLKIMGRKYTRESYLDLVNRIKKSIPNVALTTD 369
Query: 299 VIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSKKL 358
+IVG+P + + + YS R GT A M++ V + K R ++L
Sbjct: 370 IIVGYPNETEEQFEETLSLYDEVQFEHAYTYLYSQRDGTPAAKMKDNVPEDVKKARLQRL 429
Query: 359 -IELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSGKILN 417
++ +EKA +Y G+ + VL E + V GYT VN K GK++N
Sbjct: 430 NKKVGHYSEKAM-NQYEGKTVTVLCEGSSKKDDTVLAGYTEKNKLVNFKGPREAIGKLVN 488
Query: 418 VKIN 421
V+I+
Sbjct: 489 VEID 492
>MIAB_EHRRG RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 445
Score = 212 bits (539), Expect = 6e-57
Identities = 128/380 (33%), Positives = 202/380 (53%), Gaps = 7/380 (1%)
Query: 9 GCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIGRARR--Q 66
GC++N Y+S + G+ +V+ AD+ ++NTC + +K +GR R+ +
Sbjct: 10 GCQMNVYDSLIIENIIKPLGFSIVNELSEADIVILNTCHIREKAAEKLYSELGRIRKIQE 69
Query: 67 NPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVKVGAVL 125
I V GC AQ TE+ + VD+V+G ++ + + KAR KQ + + +
Sbjct: 70 TKNLTIVVAGCVAQAEGTEIFTRAPFVDIVVGPQSIHTLPELIVKARKIKKQIINIDFPV 129
Query: 126 KNKVFEDLKIEEY--NNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDEIRSL 183
+K F+ + +EEY N K AF+ +Q+GCN+FC++C++PYTRG S+ + + E L
Sbjct: 130 ISK-FDAIAVEEYTKNQKVSAFISVQEGCNKFCSFCVVPYTRGEEYSRTVEAIFKEALIL 188
Query: 184 AEHGFKEIILSGIHTASY-GVDLDEKVTLVDLLEEIEKIDGIERVRIGSIDPTFFTEDVV 242
A+ G KEI L G + +Y G + L L++ I KI IER+ + P E +
Sbjct: 189 ADSGIKEITLIGQNVNAYHGTYKGNEWDLGRLIQHIAKISSIERIYYTTSHPRDMHESLY 248
Query: 243 RRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITTDVIVG 302
KKL P HL +QSG + L++MNR++TA+EY +I+ LR D++ ++D IVG
Sbjct: 249 EAHGIEKKLIPFIHLPVQSGSNKILRKMNRKHTAEEYINIIKTLRKHRSDIAYSSDFIVG 308
Query: 303 FPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSKKLIELN 362
FPG ++ + S+ + FKYSPR GT + +Q+ K +R KL EL
Sbjct: 309 FPGETDEDFENTIRLIEEVKFSQAYSFKYSPRPGTPSAEYTSQIPDEIKSQRLTKLQELV 368
Query: 363 RINEKAFAEKYIGEVIDVLF 382
+ F +K IGE VLF
Sbjct: 369 HKQQLEFNKKMIGETHPVLF 388
>MIAB_RICFE RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 445
Score = 211 bits (538), Expect = 7e-57
Identities = 152/446 (34%), Positives = 241/446 (54%), Gaps = 18/446 (4%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIG 61
K+ T GC++N Y+S M + GYE + E ADV ++ TC + +K+ +G
Sbjct: 4 KLYIKTYGCQMNVYDSVKMQDLLYPFGYEPTENIEDADVIILITCHIREKAAEKTYSELG 63
Query: 62 RARRQNP--------EAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKAR 112
R ++ AII V GC AQ E+ S VD+V+G ++ + ++K
Sbjct: 64 RIKKLQDTRKKQGLNSAIIVVAGCVAQAEGEEIFSRAPYVDIVVGPQSYYNLPELISKVV 123
Query: 113 DEGKQQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKD 172
K + + V + K F++L + Y +F+ +Q+GC++FC +C++PYTRG+ S++
Sbjct: 124 RHEKHLIDLDFVEEAK-FDNLPEQLYPQGASSFISVQEGCDKFCTFCVVPYTRGAEFSRN 182
Query: 173 PKKVLDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKV-TLVDLLEEIEKIDGIERVRIG 230
++V E + G KEI L G + +Y G D+K+ TL DLL + +I +ER+R
Sbjct: 183 VEQVYREALKVVSSGAKEITLLGQNVNAYHGKGPDDKIFTLADLLGHLAQIPNLERLRYM 242
Query: 231 SIDPTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKI 290
+ P T+D+++ KL P HL +QSG + LK MNR++ Y DI+N LR+
Sbjct: 243 TSHPIDMTDDLIKLHGTEPKLMPFLHLPVQSGSNKILKAMNRKHDRDYYFDIINRLRETR 302
Query: 291 EDVSITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNK 350
D+ +++D IVGFPG ++R+ + + FKYSPR GT +QV +
Sbjct: 303 PDIVLSSDFIVGFPGXXXXXXXXXXXXVRRVKYGQCYSFKYSPRPGTPGATRTDQVPEHI 362
Query: 351 KDERSKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVY--TGYTRN-YIKVNAKA 407
K ER L + + AF E +G ++ VLF+ + + T Y ++ YI+ K
Sbjct: 363 KSERLTILQQELTAQQLAFNESCVGSIMKVLFDRNGKFDDQIIGKTPYMQSVYIQNPNK- 421
Query: 408 DCNVSGKILNVKINSFEGEVAEGEIV 433
++ GKI +VKI GE++
Sbjct: 422 --SLLGKITDVKITKAASNSLTGEVI 445
>MIAB_OCHA4 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 462
Score = 211 bits (538), Expect = 7e-57
Identities = 138/435 (31%), Positives = 227/435 (52%), Gaps = 17/435 (3%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIG 61
KV T GC++N Y+S+ M + EGY D + AD+ ++NTC + +K +G
Sbjct: 19 KVFVKTYGCQMNVYDSQRMADSLAAEGYVATDTPDDADLVLLNTCHIREKASEKLYSALG 78
Query: 62 RARRQNP-------EAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARD 113
R R+ E I V GC AQ E+ VD+V+G + + + + R
Sbjct: 79 RLRKMKDAREANGKELTIGVAGCVAQAEGQEILRRAPNVDLVIGPQTYHRLPNALARVRS 138
Query: 114 EGKQQVKVGAVLKNKVFEDL---KIEEYNNK-TRAFLKIQDGCNRFCAYCLIPYTRGSVC 169
G++ V+ L++K FE L K EE + AFL +Q+GC++FC +C++PYTRGS
Sbjct: 139 -GEKVVETEYALEDK-FEHLPSPKREETRKRGVSAFLTVQEGCDKFCTFCVVPYTRGSEV 196
Query: 170 SKDPKKVLDEIRSLAEHGFKEIILSGIHTASY--GVDLDEKVTLVDLLEEIEKIDGIERV 227
S+ K+++ E LA+ G +E+ L G + ++ D + L +LL + +I GI R+
Sbjct: 197 SRSVKQIVAEAERLADSGVRELTLLGQNVNAWHGAGDDGREWGLGELLFRLARIPGIARL 256
Query: 228 RIGSIDPTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLR 287
R + P + ++ L++L P+ HL +QSG D LK MNRR+ A EY ++ +R
Sbjct: 257 RYTTSHPRDMDDSLIAAHRDLRQLMPYLHLPVQSGSDRILKAMNRRHKADEYVRLIERIR 316
Query: 288 DKIEDVSITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVD 347
+ D++++ D IVGFPG ++ + ++ + FKYSPR GT ++++ V+
Sbjct: 317 EVRPDLALSGDFIVGFPGETDQDFEDTMRLVRDVNYAQAYSFKYSPRPGTPGADLDDHVE 376
Query: 348 GNKKDERSKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKA 407
KDER ++L L + AF + IG +DVL E+ + +G G + + V
Sbjct: 377 EAVKDERLQRLQALLSEQQYAFQDSMIGREMDVLLEKPGRV-AGQMVGRSPWLLPVIIDD 435
Query: 408 DCNVSGKILNVKINS 422
+ G I++VKI S
Sbjct: 436 SNDRVGDIIHVKITS 450
>MIAB_SYNJA RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 453
Score = 211 bits (537), Expect = 1e-56
Identities = 134/438 (30%), Positives = 215/438 (49%), Gaps = 21/438 (4%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIG---RA 63
T GC++N +SE M GY + AD+ + NTC++ + ++K +G +
Sbjct: 10 TFGCQMNRADSERMAGILESLGYVPAEDELQADLVLFNTCTIRDNAEQKVYSYLGIQAQR 69
Query: 64 RRQNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARD------EGK 116
+R+NP + V GC AQ + + +D+V+G + YVN+ D G
Sbjct: 70 KRKNPAIKLIVAGCVAQQEGERLLRRVPELDLVMGPQ-------YVNRLGDLLAQVEAGN 122
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
Q V V ++ ED+ +++ A++ + GCN C YC++P RG S+ P+ +
Sbjct: 123 QVVATEPV---EIPEDITKPRRDSRVTAWINVIYGCNERCTYCIVPLVRGREQSRQPQAI 179
Query: 177 LDEIRSLAEHGFKEIILSGIHTASYGVDLDEKVTLVDLLEEIEKIDGIERVRIGSIDPTF 236
EI +A G++E+ L G + +YG DLD K L LL + ++GIER+R + P +
Sbjct: 180 RAEIEDVARAGYREVTLLGQNIDAYGRDLDPKTNLASLLRFVHSVEGIERIRFATSHPRY 239
Query: 237 FTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSIT 296
FTE+++ L K+C HFH+ Q+G DA LKRM R YT + Y ++ L+R + + +I+
Sbjct: 240 FTEELIATCAELPKVCEHFHIPFQAGSDAVLKRMRRGYTQERYRQLIQLIRQYMPEATIS 299
Query: 297 TDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSK 356
D IVGFPG ++ I + YSPR GT A +QV +K +R +
Sbjct: 300 ADAIVGFPGETEAQFQETLQLVQDIGFDFLNTAAYSPRPGTPAAEWPDQVPEEEKSDRLQ 359
Query: 357 KLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADC-NVSGKI 415
+L L A + + +G+V +VL E G TR V D + G +
Sbjct: 360 RLNRLVAEVAAARSARLLGQVQEVLVEGPNPKNPRQAMGRTRGNRIVFFDGDPEQLQGSL 419
Query: 416 LNVKINSFEGEVAEGEIV 433
+ V+I + GEIV
Sbjct: 420 VPVRITATRAFSLSGEIV 437
>MIAB_SYNAS RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 461
Score = 211 bits (536), Expect = 1e-56
Identities = 141/436 (32%), Positives = 212/436 (48%), Gaps = 10/436 (2%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIGR---A 63
TLGC++N ++SE + +GY + AD+ ++NTCS+ +K++ +GR
Sbjct: 24 TLGCQMNVHDSEQIAALMEEKGYICTEDANEADLIILNTCSIREKAAQKAKSQLGRYXXX 83
Query: 64 RRQNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVKVG 122
I V GC AQ E+ + + +D + G+ N ++ ++++ K+ V+
Sbjct: 84 XXXXXXXXIGVGGCLAQQLGDELLTKVPDIDFIFGTHNIHQLPDFISRIEKSRKKIVETT 143
Query: 123 AVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDEIRS 182
L + N + +F+ I GCN FC+YC++PY RG S+ P+ ++ EIR
Sbjct: 144 LHPSTPSIGVLALP-CNGQVSSFVTIMQGCNNFCSYCIVPYVRGREESRPPEDIIHEIRM 202
Query: 183 LAEHGFKEIILSGIHTASYGVDLDEKVTLVDLLEEIEKIDGIERVRIGSIDPTFFTEDVV 242
LA+HG KE+ L G + SY ++ +LL EIEKI GIER+R + P +E ++
Sbjct: 203 LADHGVKEVTLLGQNVNSYARKTSGEMGFAELLREIEKIKGIERMRFTTSHPKDLSEFLI 262
Query: 243 RRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITTDVIVG 302
L KLC H HL QSG D L MNR YT +Y V LR D+SIT DVIVG
Sbjct: 263 TAFSDLSKLCHHIHLPFQSGSDRILALMNRGYTKSDYLAKVERLRTVCPDISITADVIVG 322
Query: 303 FPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSKKLIELN 362
FPG + +I FKYS R+GT A M+ +V K ER + L L
Sbjct: 323 FPGESDEDFKETIDMMNQIRFDNLFSFKYSEREGTAAVKMDGKVSEPLKLERLQILQALQ 382
Query: 363 RINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSGKILNVKI-- 420
+ + G+ DVL E + TG T VN ++ G +++V I
Sbjct: 383 EQHTLEKNKAMEGKQEDVLVEGFSKNCRKDLTGRTSTNKIVNFSGCVDLIGDMVSVLIKE 442
Query: 421 ---NSFEGEVAEGEIV 433
+S GE+ E+V
Sbjct: 443 AYLHSLRGEMLCEEVV 458
>MIAB_MARMM RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 456
Score = 211 bits (536), Expect = 1e-56
Identities = 138/442 (31%), Positives = 221/442 (50%), Gaps = 21/442 (4%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIGRARRQ 66
T GC++N Y+S+ M + GYE + E AD+ ++NTC + +K +GR R
Sbjct: 12 TYGCQMNVYDSDRMKDVLTPLGYESAETPEGADLVILNTCHIREKAAEKVYSELGRLRPL 71
Query: 67 NPEAI------IAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKG---EIVYYVNKARDEGK 116
E IAV GC AQ E+ VD+V+G + E++ ++A+ E
Sbjct: 72 KDEKAASGGMTIAVAGCVAQAEGAEIMKRAPVVDLVVGPQTYHKLPELIAQAHRAKGEAL 131
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
+++ D ++E Y+ AF+ +Q+GC++FC +C++PYTRG+ S+ ++
Sbjct: 132 DTEFEVEDKFDRLGSDRQVEGYS----AFVTVQEGCDKFCTFCVVPYTRGAEWSRPVDQI 187
Query: 177 LDEIRSLAEHGFKEIILSGIHTASY------GVDLDEKVTLVDLLEEIEKIDGIERVRIG 230
+ EIR+LA G +E+ L G + ++ G + + L L+ + I GIER+R
Sbjct: 188 VAEIRALAAKGIREVTLLGQNVNAFHGAPPSGREAEGAWGLGQLVRHVALIGGIERIRFT 247
Query: 231 SIDPTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKI 290
+ P E +++ KL P+FHL +Q+G D LK MNR++TA+EY I++ LR
Sbjct: 248 TSHPRDMDEVLIQAFADTPKLMPYFHLPVQAGSDKILKSMNRQHTAEEYVAIIDRLRAAR 307
Query: 291 EDVSITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNK 350
D++I+ D+IVGFPG ++R+ + FKYS R GT M NQVD
Sbjct: 308 PDIAISGDMIVGFPGETDADFEATLDLVRRVKFASCFSFKYSKRPGTPGAAMFNQVDEGV 367
Query: 351 KDERSKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCN 410
K R L EL + AF E IG + VLFE+ +G G G + V+
Sbjct: 368 KSARLAVLQELLSDQQAAFNESMIGRTLPVLFEKPGRMG-GQLHGRSPYLQSVHVDGPAE 426
Query: 411 VSGKILNVKINSFEGEVAEGEI 432
+ G++ V+I + G +
Sbjct: 427 LIGQVGEVRIEAASRNSLSGSL 448
>MIAB_UNCTG RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 418
Score = 210 bits (535), Expect = 2e-56
Identities = 130/437 (29%), Positives = 224/437 (51%), Gaps = 30/437 (6%)
Query: 1 MKVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
M T+GC++N +S+ + F G + ADV ++NTCSV + ++K+ +
Sbjct: 1 MNYLIETIGCQMNVCDSDMLVSIFSAYGASKANNLSEADVVILNTCSVRSQAEQKAFSYL 60
Query: 61 GRAR---RQNPEAIIAVVGCYAQ-IAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR + ++NP I V+GC A+ + P VD+++G+++ G +
Sbjct: 61 GRVKEFKQKNPCIKIVVIGCMAERLGPNIKKRFSSVDLIIGAKDIGNAALKIMNLFRTDY 120
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
KV + +K+K+ ++ I GC+ +C+YC +P+ RG S + + +
Sbjct: 121 SAKKVNSEIKSKIVR-------------YITIMRGCDNYCSYCAVPFVRGREVSINCETI 167
Query: 177 LDEIRSLAEHGFKEIILSGIHTASYGVDLDEKVTLVDLLEEIEKIDGIERVRIGSIDPTF 236
++E S+ ++G +EIIL G + SY E V L+++ I+ +ER+R + P
Sbjct: 168 VNECSSMVKNGAREIILLGQNVNSYQY---EDVNFASLIKKTAAIENLERIRFMTNHPKD 224
Query: 237 FTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSIT 296
++D+++ + K+CPH H+ +QS D LK MNR+Y+ + Y ++ LR + DVS+T
Sbjct: 225 LSDDLIKIMATEPKVCPHIHIPMQSASDKILKAMNRKYSYEHYLGLIKKLRTAVPDVSVT 284
Query: 297 TDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSK 356
TD+IVGFPG +K I ++F+YSPR T+A M + V +K R
Sbjct: 285 TDIIVGFPGETDEDFEDTLKAVKTIRFGGLYVFRYSPRPDTKAAEMIDDVPFEEKKRRHA 344
Query: 357 KLI-ELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSGKI 415
++ E N+I+ + +E +G VL E E+ +G+ T+N KV A+ GK
Sbjct: 345 VVLKESNKISIEIVSE-MLGSTQQVLAE---EIKNGIIKARTKNGRKVFAEGRKEYIGKH 400
Query: 416 LNV-----KINSFEGEV 427
+NV KINS G++
Sbjct: 401 INVNIKEAKINSLFGDI 417
>MIAB_THEM4 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 430
Score = 210 bits (534), Expect = 2e-56
Identities = 129/397 (32%), Positives = 214/397 (53%), Gaps = 13/397 (3%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIG 61
K+ T GC++N +SE EGYE+ + AD+ ++NTC V + K IG
Sbjct: 3 KIHIKTYGCQMNENDSEVAKFYLEEEGYEITNNENDADIVILNTCVVRKKSEDKFYSHIG 62
Query: 62 RARRQNPEAIIAVVGCYAQIAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVKV 121
++QN II ++GC A+ ++ GV V+G+R I V +A + K
Sbjct: 63 ELKKQNK--IIGIMGCGAEKEKEKLFK-RGVKFVIGTRAIPLIPQAVERAINGKKS---- 115
Query: 122 GAVLKNKVFED--LKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDE 179
A+ ++K+ E KI + N+K A++ I GCNRFC YC++PYTRG S+ +++E
Sbjct: 116 -AIFEDKMDEIDYKKILKRNSKHHAWITIIYGCNRFCTYCIVPYTRGREKSRKMDDIINE 174
Query: 180 IRSLAEHGFKEIILSGIHTASYGVDLDEKVTLVDLLEEIEKIDGIERVRIGSIDPTFFTE 239
+ +LA+ G KE+ G + +YG DL++ +L LL + I+ IER+ + PT F+
Sbjct: 175 VENLAKSGIKEVTYLGQNVDAYGKDLNDGSSLAKLLNLTKDIEEIERIWFLTSYPTDFSL 234
Query: 240 DVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITTDV 299
D+ + K+ + HL +Q G + LK+MNRRYT +EY +++N +R + D SI++D+
Sbjct: 235 DIAHEVANSSKITKNIHLPVQHGSNKILKKMNRRYTIEEYIELINDIRKIVPDASISSDI 294
Query: 300 IVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRA-ENMENQVDGNKKDERSKKL 358
IVGFP +K I + ++ YSPR+GT A + E+ V K +R +
Sbjct: 295 IVGFPDETEEDFEKTVELVKNIKFERLNLAIYSPREGTIAWKYFEDNVPRIIKTKRMAYI 354
Query: 359 IELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTG 395
+ L + K E Y+ + ++++ E + + SG+Y G
Sbjct: 355 LNLQKEINKQLNENYLNKTVEIIVETKAK--SGLYYG 389
>MIAB_ANAVT RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 454
Score = 210 bits (534), Expect = 2e-56
Identities = 134/437 (30%), Positives = 211/437 (48%), Gaps = 16/437 (3%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIGRA--- 63
T GC++N +SE M G+E + ADV + NTC++ + ++K +GR
Sbjct: 12 TFGCQMNKADSERMAGILEDMGFEFSEDPNNADVILYNTCTIRDNAEQKVYSYLGRQAKR 71
Query: 64 RRQNPEAIIAVVGCYAQI-APTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVKVG 122
+ + P+ + V GC AQ + + +D+V+G ++ + + G Q V
Sbjct: 72 KHEQPDLTLVVAGCVAQQEGEALLRRVPELDLVMGPQHANRLKDLLESVF-AGNQVVATE 130
Query: 123 AVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDEIRS 182
AV + ED+ ++ A++ + GCN C YC++P RG S+ P+ V E+
Sbjct: 131 AV---HIMEDITQARRDSTVTAWVNVIYGCNERCTYCVVPNVRGVEQSRTPEAVRAEMEE 187
Query: 183 LAEHGFKEIILSGIHTASYGVDLDEKV-------TLVDLLEEIEKIDGIERVRIGSIDPT 235
L G+KEI L G + +YG DL T DLL + + GIER+R + P
Sbjct: 188 LGRQGYKEITLLGQNIDAYGRDLPGATPEGRHLHTFTDLLYYVHDVPGIERIRFATSHPR 247
Query: 236 FFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSI 295
+FTE +++ L K+C HFH+ QSG + LK M R YT ++Y I++ +R + D SI
Sbjct: 248 YFTERLIKACAELPKVCEHFHIPFQSGDNQLLKAMARGYTQEKYRRIIDTIRRYMPDASI 307
Query: 296 TTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERS 355
+ D IVGFPG + I + + YSPR GT A ENQ+ K +R
Sbjct: 308 SADAIVGFPGETEEQFENTLKLVDDIGFDQLNTAAYSPRPGTPAALWENQLSEETKSDRL 367
Query: 356 KKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCN-VSGK 414
++L L + +++Y+G + +VL EE+ G TR K D N + G+
Sbjct: 368 QRLNHLVNVKAAERSQRYMGRIEEVLVEEQNPKDQTQVMGRTRGNRLTFFKGDINELKGQ 427
Query: 415 ILNVKINSFEGEVAEGE 431
++ VKIN GE
Sbjct: 428 LVKVKINEVRAFSLTGE 444
>MIAB_DICT6 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 440
Score = 209 bits (533), Expect = 3e-56
Identities = 141/439 (32%), Positives = 215/439 (48%), Gaps = 13/439 (2%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKK---SRQ 58
K T GC++N +SE + GY + E AD+ ++NTCSV ++K
Sbjct: 3 KYYIITYGCQMNKSDSEKVAGILENLGYTPSEKMEEADIILLNTCSVRERAEEKVFXXXX 62
Query: 59 IIGRARRQNPEAIIAVVGCYAQIAPTE-VSGIEGVDVVLGSRNKGEIVYYVNKARDEGKQ 117
I + GC AQ E + VD VLGS E+ + +GK+
Sbjct: 63 XXXXXXXXXXXXXIGIFGCMAQRMKEELIEKFPHVDFVLGSYKFTELPKILESL--DGKK 120
Query: 118 QVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVL 177
+V + + + D ++ + NK +A++ I GCN FC YC++PY RG S+DP++++
Sbjct: 121 KVVLAEDIPSPQDVDFRVIKRENKFQAWIPIIYGCNNFCTYCIVPYLRGREKSRDPEEII 180
Query: 178 DEIRSLAEHGFKEIILSGIHTASYGVDLDEKVTLVDLLEEIEKIDGIERVRIGSIDPTFF 237
E+ LA G E+ L G + SYG DL V L DLL EI +I I+R+R + P
Sbjct: 181 REVEYLASQGVVEVTLLGQNVDSYGKDLGN-VDLADLLVEIHRIPRIKRIRFLTSHPRDV 239
Query: 238 TEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITT 297
++ ++R + K+CPH+HL LQ+G D L+RM R YT EY+ ++ +R +I S +T
Sbjct: 240 SDKLIRVVATHPKVCPHWHLPLQAGSDRILRRMGRGYTYSEYKALIEKIRAEIPKASFST 299
Query: 298 DVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSKK 357
D+IVGFPG L+ I ++ YS R GT A N E+ + + + +
Sbjct: 300 DIIVGFPGEEEEDFLATRRALEEIKFDTVNLAIYSKRPGTPAANYEDLI---PYETKKRW 356
Query: 358 LIELNRINEKAFAEK---YIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSGK 414
EL + K EK +G+ VL EE +G T NY V +A+ + GK
Sbjct: 357 FDELENLQRKIIYEKNLSRVGKEEIVLAEEVNPKNPRELSGRTENYRLVFFEAEKELIGK 416
Query: 415 ILNVKINSFEGEVAEGEIV 433
L VKI +GE++
Sbjct: 417 FLLVKITEARLWSLKGEVI 435
>MIAB_RICRS RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 445
Score = 209 bits (532), Expect = 4e-56
Identities = 151/445 (33%), Positives = 242/445 (54%), Gaps = 23/445 (5%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIG 61
K+ T GC++N Y+S M + GYE + E ADV ++NTC + +K+ +G
Sbjct: 4 KLYIKTYGCQMNVYDSIKMQDLLYPFGYEPTENIEEADVIILNTCHIREKAAEKTYSELG 63
Query: 62 RARRQNP--------EAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKAR 112
R ++ AII V GC AQ E+ + VD+V+G ++ + ++K
Sbjct: 64 RIKKLQDTRTKQGLSSAIIVVAGCVAQAEGEEIFTRTPYVDIVVGPQSYYNLPELISKVV 123
Query: 113 DEGKQQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKD 172
K + + V + K F+ L + Y T AF+ +Q+GC++FC +C++PYTRG+ S++
Sbjct: 124 RHEKHLIDLDFVEEAK-FDQLPEQLYPQGTSAFISVQEGCDKFCTFCVVPYTRGAEFSRN 182
Query: 173 PKKVLDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKV-TLVDLLEEIEKIDGIERVRIG 230
++V E + G KEI+L G + +Y G +K+ +L DLL+ + +I +ER+R
Sbjct: 183 VEQVYREALKVVSSGAKEIMLLGQNVNAYHGKGPADKIFSLADLLKHLAQIPNLERLRYT 242
Query: 231 SIDPTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKI 290
+ P D+++ KL P HL +QSG + LK MNR++ + Y DI+N LR+
Sbjct: 243 TSHPIDMNNDLIKLYGTEPKLMPFLHLPVQSGSNKILKAMNRKHDREYYFDIINRLREAR 302
Query: 291 EDVSITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNK 350
D+ +++D IVGFPG ++R+ + + FKYSPR GT +Q+ +
Sbjct: 303 PDIVLSSDFIVGFPGXXXXXXXXXXXXVRRVKYGQCYSFKYSPRPGTPGATRTDQIPEHI 362
Query: 351 KDERSKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVY--TGYTRN-YIKVNAKA 407
K ER L + + AF +G + VLF+ + + T Y ++ YI+ K
Sbjct: 363 KSERLTILQQELMAQQLAFNTSCVGSTMKVLFDRNGKFDDQIIGKTPYMQSVYIQNPNK- 421
Query: 408 DCNVSGKILNVKI-----NSFEGEV 427
++ GKI++VKI NS GE+
Sbjct: 422 --SLLGKIIDVKITKASLNSLTGEI 444
>MIAB_RICRO RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 445
Score = 209 bits (532), Expect = 4e-56
Identities = 151/445 (33%), Positives = 242/445 (54%), Gaps = 23/445 (5%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIG 61
K+ T GC++N Y+S M + GYE + E ADV ++NTC + +K+ +G
Sbjct: 4 KLYIKTYGCQMNVYDSIKMQDLLYPFGYEPTENIEEADVIILNTCHIREKAAEKTYSELG 63
Query: 62 RARRQNP--------EAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKAR 112
R ++ AII V GC AQ E+ + VD+V+G ++ + ++K
Sbjct: 64 RIKKLQDTRTKQGLSSAIIVVAGCVAQAEGEEIFTRTPYVDIVVGPQSYYNLPELISKVV 123
Query: 113 DEGKQQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKD 172
K + + V + K F+ L + Y T AF+ +Q+GC++FC +C++PYTRG+ S++
Sbjct: 124 RHEKHLIDLDFVEEAK-FDQLPEQLYPQGTSAFISVQEGCDKFCTFCVVPYTRGAEFSRN 182
Query: 173 PKKVLDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKV-TLVDLLEEIEKIDGIERVRIG 230
++V E + G KEI+L G + +Y G +K+ +L DLL+ + +I +ER+R
Sbjct: 183 VEQVYREALKVVSSGAKEIMLLGQNVNAYHGKGPADKIFSLADLLKHLAQIPNLERLRYT 242
Query: 231 SIDPTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKI 290
+ P D+++ KL P HL +QSG + LK MNR++ + Y DI+N LR+
Sbjct: 243 TSHPIDMNNDLIKLYGTEPKLMPFLHLPVQSGSNKILKAMNRKHDREYYFDIINRLREAR 302
Query: 291 EDVSITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNK 350
D+ +++D IVGFPG ++R+ + + FKYSPR GT +Q+ +
Sbjct: 303 PDIVLSSDFIVGFPGXXXXXXXXXXXXVRRVKYGQCYSFKYSPRPGTPGATRTDQIPEHI 362
Query: 351 KDERSKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVY--TGYTRN-YIKVNAKA 407
K ER L + + AF +G + VLF+ + + T Y ++ YI+ K
Sbjct: 363 KSERLTILQQELMAQQLAFNTSCVGSTMKVLFDRNGKFDDQIIGKTPYMQSVYIQNPNK- 421
Query: 408 DCNVSGKILNVKI-----NSFEGEV 427
++ GKI++VKI NS GE+
Sbjct: 422 --SLLGKIIDVKITKASLNSLTGEI 444
>MIAB_PETMO RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 439
Score = 209 bits (532), Expect = 4e-56
Identities = 132/439 (30%), Positives = 236/439 (53%), Gaps = 10/439 (2%)
Query: 1 MKVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
MK T GC++N ESE M EG+E + + AD+ ++N+C+V + K I
Sbjct: 1 MKFYIRTFGCQMNINESEIMAGLLKEEGFEWTENPKEADIILINSCAVREKAENKMYGAI 60
Query: 61 G---RARRQNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
G + + +N I+ V GC A+ + + +D V G+RN +I V +A + GK
Sbjct: 61 GGYGKLKDENKNLILGVGGCSAEKERENLLERFKNIDFVFGTRNVVDIGNLVKRALN-GK 119
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
+ L + ++ K+ +K A++ I GCN++C+YC++PYTRG S+ + +
Sbjct: 120 RFADFSDKLNDVNYDIPKMPI--SKHHAWITIIYGCNKYCSYCIVPYTRGFEKSRPMEDI 177
Query: 177 LDEIRSLAEHGFKEIILSGIHTASYGVDLDEKVTLVDLL-EEIEKIDGIERVRIGSIDPT 235
+ E+ S A+ G+KEI G + SYG D +K + +DLL ++ + D I+R+ + P+
Sbjct: 178 IREVESYAKKGYKEITFLGQNVDSYGKDFGDKKSKLDLLIQKAAEFDSIKRIWFLTSYPS 237
Query: 236 FFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSI 295
T+ +++ + +K +FHL QSG + LK MNR+YT +E+ ++VN ++ ++ +V++
Sbjct: 238 DITDSLIQTVANEEKAANYFHLPAQSGSNKILKAMNRKYTREEFIELVNKVKKEVANVTV 297
Query: 296 TTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMEN-QVDGNKKDER 354
++D I GFP +K+ + +I +YSPR+GT A +N V + K++R
Sbjct: 298 SSDFITGFPSETDEDFEETVDLIKQCRFERINIAEYSPREGTIAYKYQNDDVPKHIKNKR 357
Query: 355 SKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSGK 414
+ L+EL + EKY+ + + V+ +E +G Y G T N + +++ ++G+
Sbjct: 358 LQYLMELQKRINLEENEKYLEQEV-VIIQEGKAGKNGTYMGRTMNNKLIIFESNEELNGE 416
Query: 415 ILNVKINSFEGEVAEGEIV 433
L VK+N GE+V
Sbjct: 417 FLKVKVNKITPGPLYGEVV 435
>MIAB_BACSK RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 520
Score = 209 bits (532), Expect = 4e-56
Identities = 140/439 (31%), Positives = 220/439 (50%), Gaps = 11/439 (2%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIG 61
K T GC++N ++SE M+ + G+ AD+ ++NTC++ + K IG
Sbjct: 78 KFLIRTYGCQMNVHDSENMSGLLLGMGFXXXXXXXXADIILLNTCAIRENAENKVFGEIG 137
Query: 62 RARRQNPEA---IIAVVGCYAQ---IAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEG 115
II V GC +Q + + + +D++ G+ N + + + A G
Sbjct: 138 NXXXXXXXXXXXIIGVCGCMSQEESVVGKIMEKHQHIDLIFGTHNIHRLPHLLRDAIF-G 196
Query: 116 KQQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKK 175
K+ V + + E++ NKT+A++ I GC++FC YC++PYTRG S+ P+
Sbjct: 197 KEMVIEVWSKEGDIVENMP-RSRKNKTQAWVNIMYGCDKFCTYCIVPYTRGKERSRLPED 255
Query: 176 VLDEIRSLAEHGFKEIILSGIHTASYGVDLDEKVTLVDLLEEIEKIDGIERVRIGSIDPT 235
++ E+R LA G+KEI L G + +YG DL L DL+ EI KID I RVR + P
Sbjct: 256 IIAEVRDLARQGYKEITLLGQNVNAYGKDLPGSYRLGDLMNEIHKID-IPRVRFTTSHPR 314
Query: 236 FFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSI 295
F + ++ + L H HL +Q G LK M R+YT ++Y + N ++ I + S
Sbjct: 315 DFDDHLIEVLAQGGNLVEHIHLPVQHGNSDILKLMGRKYTREQYITLANKIKQAIPNASF 374
Query: 296 TTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERS 355
TTD+IVGFP ++ I + + YSPR+GT A ME+ V + K ER
Sbjct: 375 TTDLIVGFPNETEEQFQDMLSLVEEIQFDAAYTYIYSPREGTPAARMEDNVPMSVKKERL 434
Query: 356 KKLIEL-NRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSGK 414
+L L N I+ K + Y ++++VL E E + + V G TR VN + G+
Sbjct: 435 ARLNALVNDISNKRNLD-YQDKIVEVLVEGESKKNTDVLAGRTRTNRLVNFVGPKSAIGE 493
Query: 415 ILNVKINSFEGEVAEGEIV 433
I+ VKI + +GEIV
Sbjct: 494 IVYVKITEAKTWSLDGEIV 512
>MIAB_ARCB4 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 436
Score = 209 bits (531), Expect = 5e-56
Identities = 142/446 (31%), Positives = 228/446 (51%), Gaps = 30/446 (6%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVR-EGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ TLGC++N +S+ + + + +GY E AD+ ++NTCSV +K I
Sbjct: 6 KLFIQTLGCQMNDTDSQHIQAELEKHKGYVTTQNIEDADLIIINTCSVRERPVQKLFSEI 65
Query: 61 GRARRQNPE-AIIAVVGCYAQ-IAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQ 118
G+ ++ E A I V GC A + + VD V+G+RN +I K
Sbjct: 66 GQFNKKKKEGAKIGVCGCTASHLGQDIIKRAPYVDFVVGARNISKI-----------KDV 114
Query: 119 VKVGAVLKNKVFEDLKIEEYN----NKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPK 174
V V ++ + D E++ NK RA + I GC++ C YC++P TRG S P+
Sbjct: 115 VDVKGAVEVSIDNDESTYEFSTAKTNKYRASVNISVGCDKKCTYCIVPSTRGEEISIPPE 174
Query: 175 KVLDEIRSLAEHGFKEIILSGIHTASYG---VDLDEKVTLVDLLEEIEKIDGIERVRIGS 231
+++++R E G E++L G + SYG D EK T LL+++ KIDG+ER+R S
Sbjct: 175 MIVEQVRKSVEQGAVEVMLLGQNVNSYGRKFSDKREKYTFTKLLQDVSKIDGLERIRFTS 234
Query: 232 IDPTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIE 291
P ++ + K+ H+ LQSG + LK M R Y+ + + + + +R+ +
Sbjct: 235 PHPLHMDDEFIEEFAKNPKISKCIHMPLQSGSTSVLKAMKRGYSKEWFLNRASKMRELVP 294
Query: 292 DVSITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKK 351
++ ITTD+IV FPG ++++ + FKYSPR GT A N++ D
Sbjct: 295 NLRITTDIIVAFPGETQEDFLDTLDVVEQVKFDQIFNFKYSPRPGTEALNLK---DKELP 351
Query: 352 DE-RSKKLIELNRINEKAFAE---KYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKA 407
DE S++LI+L ++++ E K IGE +++L E +G GYT NY++V AK
Sbjct: 352 DEIGSQRLIDLIELHKRYLEESMPKLIGETLNILVESLKP--NGEVCGYTDNYLQVFAKG 409
Query: 408 DCNVSGKILNVKINSFEGEVAEGEIV 433
+ GK +NVKI +GE+V
Sbjct: 410 SDELLGKFVNVKITDVTRTSLKGEVV 435
>MIAB_SYNP2 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 451
Score = 208 bits (530), Expect = 6e-56
Identities = 130/439 (29%), Positives = 213/439 (48%), Gaps = 16/439 (3%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIGRA--- 63
T GC++N +SE M GY + AD+ + NTC++ + ++K +GR
Sbjct: 11 TFGCQMNKADSERMAGILEEMGYHFTEDPYAADLVLYNTCTIRDNAEQKVYSYLGRQAKR 70
Query: 64 RRQNPEAIIAVVGCYAQI-APTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVKVG 122
+++ P+ + V GC AQ + + + +D+++G ++ + + + EG QV
Sbjct: 71 KQEKPDLTLIVAGCVAQQEGESLLRRVPELDLIMGPQHANRLQDLLEQV--EGGSQVVAT 128
Query: 123 AVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDEIRS 182
+ + ED+ ++ A++ + GCN C YC++P RG+ S+ P+ + E+
Sbjct: 129 EPIH--IVEDITKPRRDSTVTAWVNVIYGCNEHCTYCVVPGVRGTEQSRYPEAIRAEMEE 186
Query: 183 LAEHGFKEIILSGIHTASYGVDLD-------EKVTLVDLLEEIEKIDGIERVRIGSIDPT 235
L GFKE+ L G + +YG DL K TL DLL + + GIER+R + P
Sbjct: 187 LGRQGFKEVTLLGQNIDAYGRDLPGTTPEGRNKYTLTDLLYYVHDVPGIERIRFATSHPR 246
Query: 236 FFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSI 295
+FTE +++ L K+C HFH+ QSG + LK M R YT ++Y I+N +R + D SI
Sbjct: 247 YFTERLIKACQELPKVCEHFHIPFQSGDNEVLKAMRRGYTHEKYRRIINTIRSYMPDASI 306
Query: 296 TTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERS 355
+ D IV FPG + I + + YSPR GT A +NQ+ K +R
Sbjct: 307 SADAIVAFPGETEEQFENTLKLVDEIGFDQLNTAAYSPRPGTPAATWDNQLSEEVKGDRL 366
Query: 356 KKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCN-VSGK 414
++L L +++Y G + +VL E++ G TR + + N + GK
Sbjct: 367 QRLNHLVAQKAAERSQRYAGRIEEVLVEDQNPKNPSQVMGRTRGNRLTFFEGEINELKGK 426
Query: 415 ILNVKINSFEGEVAEGEIV 433
I+ VKI GE V
Sbjct: 427 IVAVKITEARAFSLTGEAV 445
>MIAB_SYNJB RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 444
Score = 208 bits (530), Expect = 6e-56
Identities = 131/438 (29%), Positives = 216/438 (49%), Gaps = 21/438 (4%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIG---RA 63
T GC++N ++E M GY + AD+ + NTC++ + ++K +G +
Sbjct: 10 TFGCQMNRADTERMAGILESLGYVATEDELQADLVLYNTCTIRDNAEQKVYSYLGIQAQR 69
Query: 64 RRQNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARD------EGK 116
+R+NP + V GC AQ ++ + +D+V+G + YVN+ D G
Sbjct: 70 KRKNPAIKLIVAGCVAQQEGEKLLRRVPELDLVMGPQ-------YVNRLGDLLAQVEAGN 122
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
Q V V ++ ED+ +++ A++ + GCN C YC++P RG S+ P+ +
Sbjct: 123 QVVATDPV---EIPEDITKPRRDSQVTAWINVIYGCNERCTYCIVPRVRGQEQSRQPQAI 179
Query: 177 LDEIRSLAEHGFKEIILSGIHTASYGVDLDEKVTLVDLLEEIEKIDGIERVRIGSIDPTF 236
EI +A G++E+ L G + +YG DLD K L LL + ++GIER+R + P +
Sbjct: 180 RAEIEDVARAGYREVTLLGQNIDAYGRDLDPKTNLASLLRFVHSVEGIERIRFATSHPRY 239
Query: 237 FTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSIT 296
FTE+++ L K+C HFH+ Q+G + LKRM R YT + Y I+ L+R + + +I+
Sbjct: 240 FTEELITTCAELPKVCEHFHIPFQAGSNEVLKRMRRGYTHERYRQIIQLIRQYMPEAAIS 299
Query: 297 TDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSK 356
D IVGFPG ++ I + + YSPR GT A +QV +K +R +
Sbjct: 300 ADAIVGFPGETEAQFQETLQLVQDIGFDQVNTAAYSPRPGTPAAEWPDQVPEEEKSDRLQ 359
Query: 357 KLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADC-NVSGKI 415
+L L A + + +G+V +VL E G TR V + D + G +
Sbjct: 360 RLNRLVAEVAAARSARLLGQVQEVLVEGPNPKNPRQAMGRTRGNRLVFFEGDPEELQGSL 419
Query: 416 LNVKINSFEGEVAEGEIV 433
+ V+I + GE V
Sbjct: 420 VPVRITATRAFSLTGEAV 437
>MIAB_SYNFM RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 456
Score = 208 bits (530), Expect = 6e-56
Identities = 138/433 (31%), Positives = 210/433 (48%), Gaps = 8/433 (1%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIGRARR- 65
T GC++N Y+S+ GY ADV +NTCSV + ++K +GR RR
Sbjct: 19 TFGCQMNEYDSQRALRLLCAVGYRPTSDIADADVIFLNTCSVRDKAEQKVYSFLGRLRRL 78
Query: 66 --QNPEAIIAVVGCYAQ-IAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVKVG 122
P I V GC AQ + + E VD+V+G+R G I + + ++ +
Sbjct: 79 KAHRPWLKIVVAGCVAQQLGDGLLKRFEHVDLVVGTRGIGSIASLLEEVERSKRRVAHLP 138
Query: 123 AVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDEIRS 182
A D A + I GCN FC YC++P+ RG S+ P +L EI
Sbjct: 139 AEELQGFTTDKCRTVGTGDVVAQVTIMQGCNNFCTYCIVPHVRGRERSRAPDDILREIDF 198
Query: 183 LAEHGFKEIILSGIHTASYGVDLDEKVTLVDLLEEIEKIDGIERVRIGSIDPTFFTEDVV 242
LA G +E++L G + SYG L + ++ DLL I K I RVR + P TED++
Sbjct: 199 LASRGAREVLLLGQNVNSYGRGLPDPISFPDLLRRIGKETSIRRVRFTTSHPKDLTEDLI 258
Query: 243 RRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITTDVIVG 302
L LC H HL QSG D LK M+R YTA++Y + + LR+ +++++TDVIVG
Sbjct: 259 ECFAGLPFLCKHLHLPFQSGSDGILKLMHRGYTARQYLEKIARLREVCPEIALSTDVIVG 318
Query: 303 FPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSKKL--IE 360
FP ++ + F+YS R TRA ++V + K R +L I+
Sbjct: 319 FPAESEEDYLQTLRLIEEVRFDSLFSFRYSDRPLTRAAGFPDKVPMDVKVRRLARLQSIQ 378
Query: 361 LNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSGKILNVKI 420
+ +K AE G V +VL E + +G TG T+ +N + +++GKI+ V+I
Sbjct: 379 ADITLQKNLAE--TGTVREVLVEGPSKASNGQMTGRTQQNRIINFQCPVDLTGKIVPVRI 436
Query: 421 NSFEGEVAEGEIV 433
+ +GE++
Sbjct: 437 VAAYSHSLKGELL 449
>MIAB_SILST RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 440
Score = 208 bits (530), Expect = 6e-56
Identities = 139/444 (31%), Positives = 223/444 (50%), Gaps = 22/444 (4%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIG 61
K+ T GC++N Y+SE M E +GY + AD+ ++NTC + +K +G
Sbjct: 6 KLYIKTYGCQMNVYDSERMAETLGGQGYVETQTPDDADMILLNTCHIREKAAEKVYSELG 65
Query: 62 R---ARRQNPEAIIAVVGCYAQIAPTEVSGIEG-VDVVLGSRNKGEIVYYVNKARDEGKQ 117
R + + P+ I V GC AQ E+ + VD+V+G ++ + KA K
Sbjct: 66 RFKGLKAEKPDLKIGVAGCVAQAEGEEIMRRQPLVDLVVGPQSYHRLPEMEAKAGTGEK- 124
Query: 118 QVKVGAVLKNKVFEDLKIEEYNNKTRA------FLKIQDGCNRFCAYCLIPYTRGSVCSK 171
VL E+ K E+ + +A FL +Q+GC++FCA+C++PYTRG+ S+
Sbjct: 125 ------VLDTDFPEEDKFEKLKRRPKAKRGPTAFLTVQEGCDKFCAFCVVPYTRGAEVSR 178
Query: 172 DPKKVLDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKVTLVDLLEEIEKIDGIERVRIG 230
+VL E L E G +EI L G + +Y G + +TL L+ E++KIDG+ER+R
Sbjct: 179 PVDRVLREAEDLVERGVREITLLGQNVNAYHGAGPNGDMTLAQLIWELDKIDGLERIRFT 238
Query: 231 SIDPTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKI 290
+ P +D++ KKL P+ HL +Q+G D LKRMNR +TA+ Y ++ +R
Sbjct: 239 TSHPNDMMDDLIEAHGTCKKLMPYLHLPVQAGSDKILKRMNRSHTAESYIRLIERIRAAR 298
Query: 291 EDVSITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNK 350
D+ I+ D IVGFP ++ + + FKYS R GT A QVD +
Sbjct: 299 PDILISGDFIVGFPEETEEDFQATLDLVEEVKYGTAYSFKYSTRPGTPAAE-RAQVDPKE 357
Query: 351 KDERSKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCN 410
D+R ++L + ++ + +G + VLFE+ +G G + +Y+ ADC+
Sbjct: 358 ADDRLQRLQAVLTRQQREVQDSMVGRELGVLFEKAGRF-AGQMVGKS-DYLHAVHVADCD 415
Query: 411 VS-GKILNVKINSFEGEVAEGEIV 433
G + V+I S GE++
Sbjct: 416 AKIGDLRRVRIVSSGANSLAGELI 439
>MIAB_ANAPZ RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 444
Score = 208 bits (530), Expect = 6e-56
Identities = 138/432 (31%), Positives = 229/432 (53%), Gaps = 16/432 (3%)
Query: 9 GCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIGRARR-QN 67
GC++N Y++ M + GY V E AD+ ++NTC + +K +GR R +
Sbjct: 11 GCQMNVYDALMMEDLLRPVGYAAVSRPEDADIILINTCHIREKASEKLYSTLGRMRVIKK 70
Query: 68 PEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVKVGAVLK 126
E I+ V GC AQ V V+VV+G + + + K + + K+Q+ + +
Sbjct: 71 EECILIVAGCVAQAEGEAVFERAPYVNVVVGPQGLHTLPELIMKVKRD-KKQINIEFPVV 129
Query: 127 NKVFEDLKIE-EYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDEIRSLAE 185
+K F+ + I+ + N A++ IQ+GC++FC +C++PYTRG S+D + +L+E++ L +
Sbjct: 130 SK-FDAISIDRKANGGVSAYVSIQEGCDKFCTFCVVPYTRGPEYSRDVEAILEEVKQLTD 188
Query: 186 HGFKEIILSGIHTASY-GVDLDEKVTLVDLLEEIEKIDGIERVRIGSIDPTFFTEDVVRR 244
G KEI+L G + +Y G + L L++++ IDG+ER+R + P +
Sbjct: 189 RGTKEIVLIGQNVNAYHGTYKGNEWDLGKLIQKVSLIDGVERIRYTTSHPRDMHPSLYEA 248
Query: 245 ILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITTDVIVGFP 304
KKL P HL +QSG DA L++MNR++TA+EY +V L+D +++++++D IVGFP
Sbjct: 249 HRDEKKLAPFVHLPVQSGSDAILRKMNRKHTAEEYLRVVEQLKDSNKNMALSSDFIVGFP 308
Query: 305 GXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSKKLIELNRI 364
G ++ + + ++ FKYSPR GT NQV +K R L L
Sbjct: 309 GETEKDFEETMKLVESVGFALSYSFKYSPRPGTPGAEYSNQVPEEEKSARLAALQGLLTK 368
Query: 365 NEKAFAEKYIGEVIDVLFEEEVELGSGVYTG---YTRNYIKVNAKA---DC---NVSGKI 415
+ F + G V+DVL + + S G YT++ I ++A + DC V +I
Sbjct: 369 QQLQFNKSMEGRVMDVLVGDPSSMRSDRIFGKSEYTQS-IHISAPSGSEDCFNRMVRVEI 427
Query: 416 LNVKINSFEGEV 427
L+ + NS EG V
Sbjct: 428 LHGRQNSLEGTV 439
>MIAB_RICCN RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 445
Score = 208 bits (529), Expect = 8e-56
Identities = 151/445 (33%), Positives = 241/445 (54%), Gaps = 23/445 (5%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIG 61
K+ T GC++N Y+S M + GYE + E ADV ++NTC + +K+ +G
Sbjct: 4 KLYIKTYGCQMNVYDSVKMQDLLYPFGYEPTENIEEADVIILNTCHIREKAAEKTYSELG 63
Query: 62 RARRQNP--------EAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKAR 112
R ++ AII V GC AQ E+ + VD+V+G ++ + ++K
Sbjct: 64 RIKKLQDTRTKQGLSSAIIVVAGCVAQAEGEEIFTRTPYVDIVVGPQSYYNLPELISKVV 123
Query: 113 DEGKQQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKD 172
K + + V + K F+ L + Y T AF+ +Q+GC++FC +C++PYTRG+ S++
Sbjct: 124 RHEKHLIDLDFVEEAK-FDQLPEQLYPQGTSAFISVQEGCDKFCTFCVVPYTRGAEFSRN 182
Query: 173 PKKVLDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKV-TLVDLLEEIEKIDGIERVRIG 230
++V E + G KEI+L G + +Y G +K+ +L DLL+ + +I +ER+R
Sbjct: 183 VEQVYREALKVVSSGAKEIMLLGQNVNAYHGKGPADKIFSLADLLKHLAQIPNLERLRYT 242
Query: 231 SIDPTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKI 290
+ P D+++ KL P HL +QSG + LK MNR++ + Y DI+N LR+
Sbjct: 243 TSHPIDMNNDLIKLYGTEPKLMPFLHLPVQSGSNKILKAMNRKHDREYYFDIINRLREAR 302
Query: 291 EDVSITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNK 350
D+ +++D IVGFPG ++R+ + + FKYSPR GT +Q+ +
Sbjct: 303 PDIVLSSDFIVGFPGXXXXXXXXXXXXVRRVKYGQCYSFKYSPRPGTPGATRTDQIPEHI 362
Query: 351 KDERSKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVY--TGYTRN-YIKVNAKA 407
K ER L + + AF +G + VLF+ + + T Y ++ YI+ K
Sbjct: 363 KSERLTILQQELMAQQLAFNTSCVGSTMKVLFDRNGKFDDQIIGKTPYMQSVYIQNPNK- 421
Query: 408 DCNVSGKILNVKI-----NSFEGEV 427
++ GKI +VKI NS GE+
Sbjct: 422 --SLLGKISDVKITKASLNSLTGEI 444
>MIAB_BURPP RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 457
Score = 208 bits (529), Expect = 8e-56
Identities = 143/446 (32%), Positives = 212/446 (47%), Gaps = 15/446 (3%)
Query: 2 KVAFATLGCRVNTYESEAMTEKF-VREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
KV T GC++N Y+S+ M + EG D E ADV + NTCSV +K +
Sbjct: 4 KVYVKTFGCQMNEYDSDKMVDVLGAAEGLVKTDTPEDADVILFNTCSVREKAQEKVFSDL 63
Query: 61 GRARR---QNPEAIIAVVGCYA-QIAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR R NP II V GC A Q VS VD+V G + + ++K R+ G+
Sbjct: 64 GRVRELKEANPNLIIGVGGCVASQEGAAIVSRAPYVDLVFGPQTLHRLPQMIDKRRESGR 123
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
QV + K F+ L + + AF+ I +GC+++C+YC++PYTRG S+ V
Sbjct: 124 AQVDISFPEIEK-FDHLPPARVDGPS-AFVSIMEGCSKYCSYCVVPYTRGEEVSRPLDDV 181
Query: 177 LDEIRSLAEHGFKEIILSGIHTASYGVDLD----EKVTLVDLLEEIEKIDGIERVRIGSI 232
L EI LA+ G +E+ L G + +Y L E L+E + I GIER+R +
Sbjct: 182 LTEIAGLADQGVREVTLLGQNVNAYRAGLTLGSTEIADFAQLIEYVADIPGIERIRYTTS 241
Query: 233 DPTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIED 292
P FT+ ++ + KL H HL +Q G D L M R YT EY+ ++ LR D
Sbjct: 242 HPKEFTQRLIDTYAKVPKLVSHLHLPVQHGSDRILMAMKRGYTVLEYKSVIRKLRAIRPD 301
Query: 293 VSITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKD 352
+S++TD+IVGFPG + + + F YSPR GT A N+ + K
Sbjct: 302 LSLSTDMIVGFPGETEEDFDKMMALVHEMKYDTSFSFIYSPRPGTPAANLHDDTPREVKL 361
Query: 353 ERSKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKA----D 408
+R + L N + ++ +G++ +L E G T N VN A
Sbjct: 362 KRLQHLQATIEENVQRISDSMVGKIERILVERPARKDPNELAGRTENNRVVNFPAPIASH 421
Query: 409 CNVSGKILNVKINSFEGEVAEGEIVL 434
+ G++++VKI GE+VL
Sbjct: 422 ARLIGQMVDVKIVKAYPHSLRGELVL 447
>MIAB_RHIME RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 472
Score = 207 bits (528), Expect = 1e-55
Identities = 134/432 (31%), Positives = 222/432 (51%), Gaps = 15/432 (3%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIG 61
KV T GC++N Y+S+ M++ R+GY D E AD ++NTC + +K +G
Sbjct: 23 KVFVKTYGCQMNVYDSDRMSDALSRDGYVATDVLEDADFVLLNTCHIREKAAEKVYSELG 82
Query: 62 RARR-------QNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARD 113
R R + E +I V GC AQ E+ + VD+V+G + + + +AR
Sbjct: 83 RLRELKKAKALEGREMLIGVAGCVAQAEGDEILRRVPAVDLVIGPQTYHRLPDALKRAR- 141
Query: 114 EGKQQVKVGAVLKNKVFEDLKIEEYNNKTR---AFLKIQDGCNRFCAYCLIPYTRGSVCS 170
G++ V+ +++K ++ + R AFL +Q+GC++FC +C++PYTRGS S
Sbjct: 142 AGQRIVETEYAIEDKFVHLPAPDKAKTRARGVTAFLTVQEGCDKFCTFCVVPYTRGSEVS 201
Query: 171 KDPKKVLDEIRSLAEHGFKEIILSGIHTASYGVD--LDEKVTLVDLLEEIEKIDGIERVR 228
+ +++ E L E G +EI L G + ++ + + +L DLL + +IDG+ R+R
Sbjct: 202 RPVAQIVAEAEKLVEGGVREITLLGQNVNAWHGEGPHGREWSLGDLLRRLGEIDGLARLR 261
Query: 229 IGSIDPTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRD 288
+ P + ++ ++ KL P+ HL +QSG D LK MNRR+TA EY +V +R
Sbjct: 262 YTTSHPRDMDDSLIEAHRSMAKLMPYLHLPVQSGSDRILKAMNRRHTAAEYLALVERIRA 321
Query: 289 KIEDVSITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDG 348
D++++ D IVGFPG ++ + ++ FKYS R GT ++ QV
Sbjct: 322 AQPDLALSGDFIVGFPGETDQDFEDTLRLVEEVNYAQAFSFKYSTRPGTPGAELKEQVPE 381
Query: 349 NKKDERSKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKAD 408
+ K +R + L L ++ FAE +G ID+L E+ + G G + VN A
Sbjct: 382 DVKAKRLEILQALLVKQQRGFAEACVGREIDLLLEKPGRM-PGQLVGRSPWLQPVNVDAK 440
Query: 409 CNVSGKILNVKI 420
+ G I+ V+I
Sbjct: 441 ASQIGDIIRVRI 452
>MIAB_EHRCJ RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 441
Score = 207 bits (528), Expect = 1e-55
Identities = 128/383 (33%), Positives = 197/383 (51%), Gaps = 7/383 (1%)
Query: 9 GCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIGRARR--Q 66
GC++N Y+S M G+ VV ADV ++NTC + +K +G+ R+ +
Sbjct: 10 GCQMNVYDSLIMENIIKPLGFTVVSEPSEADVVILNTCHIREKASEKLYSELGKIRKIQE 69
Query: 67 NPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVKVGAVL 125
N + I V GC AQ E+ VD+V+G ++ + + KA KQ + + +
Sbjct: 70 NKDLTIVVAGCVAQAEGEEIFERSPFVDIVVGPQSIHTLPELLVKAHRIKKQVINIDFPV 129
Query: 126 KNKVFEDLKIEEY--NNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDEIRSL 183
+K F+ + +EEY N +T AF+ +Q+GCN+FC +C++PYTRG S+ + + +E L
Sbjct: 130 ISK-FDAIPVEEYTKNQETSAFISVQEGCNKFCTFCVVPYTRGEEYSRTVEAIFNEALVL 188
Query: 184 AEHGFKEIILSGIHTASY-GVDLDEKVTLVDLLEEIEKIDGIERVRIGSIDPTFFTEDVV 242
A+ G KEI L G + +Y G + L L++ I KI IER+R + P + +
Sbjct: 189 ADSGIKEITLIGQNVNAYHGTYKGCEWDLGKLIQHIAKIPNIERIRYTTSHPRDMHQSLY 248
Query: 243 RRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITTDVIVG 302
+KL P HL +QSG D LK+MNR++TA+EY DI++ LR + D++ ++D IVG
Sbjct: 249 EAHKLEEKLMPFVHLPVQSGSDRILKKMNRKHTAEEYIDIISHLRKQRPDIAFSSDFIVG 308
Query: 303 FPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSKKLIELN 362
FPG ++++ S+ + FKYSPR GT NQV K
Sbjct: 309 FPGETEEDFENTIKLVEKVKFSQAYSFKYSPRPGTPGAEYPNQVPEEIKSXXXXXXXXXX 368
Query: 363 RINEKAFAEKYIGEVIDVLFEEE 385
AF IGE VLF +
Sbjct: 369 XXXXXAFNRSMIGETCTVLFSSK 391
>MIAB_DESPS RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 447
Score = 207 bits (528), Expect = 1e-55
Identities = 125/393 (31%), Positives = 201/393 (51%), Gaps = 19/393 (4%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIGRARRQ 66
T GC++N +SE + + GY AD+ ++NTCS+ ++K +G RR
Sbjct: 10 TYGCQMNLRDSEIIAQILNNNGYVETSEIGGADLVLLNTCSIRAKAEQKVMSKLGELRRN 69
Query: 67 ---NPEAIIAVVGCYAQIAPTEVSG-IEGVDVVLGSRNKGEIVYYVNKARDEGKQQVKVG 122
NP I V GC AQ ++ + VD+V+G+ + +Y +N+ + + + +
Sbjct: 70 KKINPRMQICVAGCVAQQEGKQIQAKMPHVDLVIGT----QYIYAINELLERSRTEGPIT 125
Query: 123 AV-LKNKV-----FEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
A L +K + +E+ + R F+ I GCN FC YC++PYTRG S+ K +
Sbjct: 126 ATNLDDKYVIPQFIPETTGKEHEGEFRKFVTIMQGCNNFCTYCVVPYTRGREVSRSIKDI 185
Query: 177 LDEIRSLAEHGFKEIILSGIHTASYG-----VDLDEKVTLVDLLEEIEKIDGIERVRIGS 231
++EI L + G KEI L G + SY + D T DLL ++ ++G++R+R +
Sbjct: 186 VEEITVLVKSGIKEITLLGQNVNSYAQTNTVTEDDTPATFSDLLRQVAAVEGLKRLRFTT 245
Query: 232 IDPTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIE 291
+P + D+++ L LCP FHL +Q+G + LK M R+YT + Y D+V LR+
Sbjct: 246 SNPKDLSNDLMQCFKDLDVLCPQFHLPVQAGSNKVLKEMGRKYTVESYLDLVTQLRENCP 305
Query: 292 DVSITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKK 351
+++ITTD+IVGFPG L+ + + FKYS R GT+A + N+VD + K
Sbjct: 306 EIAITTDIIVGFPGETDEEFEETMKMLETVRYHGSFSFKYSDRPGTKANELTNKVDESVK 365
Query: 352 DERSKKLIELNRINEKAFAEKYIGEVIDVLFEE 384
R + ++YIG +VL EE
Sbjct: 366 SARLARFQARQDEIGLERNQEYIGTTQEVLIEE 398
>MIAB_DECAR RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 445
Score = 207 bits (527), Expect = 1e-55
Identities = 142/444 (31%), Positives = 217/444 (48%), Gaps = 16/444 (3%)
Query: 2 KVAFATLGCRVNTYESEAMTEKF-VREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S+ M + EG D E AD+ + NTCSV +K +
Sbjct: 4 KLFIRTFGCQMNEYDSDKMADVLNASEGVIKTDNPEEADIILFNTCSVREKAQEKVFHDL 63
Query: 61 GRAR---RQNPEAIIAVVGCYA-QIAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR R + NP +I V GC A Q ++ VDVV G + + + + + +GK
Sbjct: 64 GRVRHLKKLNPNLVIGVGGCVASQEGDAIIARAPYVDVVFGPQTLHRLPQLIAERKAKGK 123
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
V V K F+ + E + AF+ I +GC++FC +C++PYTRG S+ V
Sbjct: 124 AAVDVSFPEIEK-FDAMPPSEIKGAS-AFVSIMEGCSKFCTFCIVPYTRGGEVSRPFNDV 181
Query: 177 LDEIRSLAEHGFKEIILSGIHTASYGVDLD---EKVTLVDLLEEIEKIDGIERVRIGSID 233
L E+ LA HG E+ L G + +Y D++ EK L L+E I ++ GIER+R +
Sbjct: 182 LTEVADLAAHGVGEVTLLGQNVNAYRGDMEGTEEKADLALLIEYIAEVPGIERIRYTTSH 241
Query: 234 PTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDV 293
P T+ + + + KL H HL +Q+G D L M R YT EY+ I+ LR D+
Sbjct: 242 PREMTQSLFDTYVKVPKLVSHLHLPVQAGSDRVLAAMKRGYTTLEYKSIIRKLRAARPDI 301
Query: 294 SITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDE 353
S+++D IVGFPG + + + F YSPR GT A ++ D D
Sbjct: 302 SLSSDFIVGFPGETDEDFEKTMKLIDDVGFDSSFSFIYSPRPGTPALELD---DSTPADV 358
Query: 354 RSKKLIEL-NRINE--KAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCN 410
+S +L+ L RI+E +A ++ +G + VL E + G T N VN +
Sbjct: 359 KSARLLRLQKRIDEQAQAISQTMVGSIQRVLVEGTSKKDVHELAGRTDNNRIVNFSGNPR 418
Query: 411 VSGKILNVKINSFEGEVAEGEIVL 434
+ ++V+I S GEIV+
Sbjct: 419 LINTFVDVRITSSLSHTLRGEIVI 442
>MIAB_BUCBP RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 445
Score = 207 bits (527), Expect = 1e-55
Identities = 135/438 (30%), Positives = 222/438 (50%), Gaps = 13/438 (2%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVRE-GYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S +T+ ++ GYE ++A+V ++NTCS+ +K +
Sbjct: 5 KIYIKTWGCQMNEYDSALITQILKQKHGYENTKDPKLANVLILNTCSIREKAQEKVFHQL 64
Query: 61 GR---ARRQNPEAIIAVVGCYA-QIAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR +++NP IIAV GC A Q VD++ G++ ++Y+ E K
Sbjct: 65 GRWKKLKQKNPNLIIAVGGCVATQEGKNIYKRANYVDIIFGTQT----LHYLPNMIQEVK 120
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTR--AFLKIQDGCNRFCAYCLIPYTRGSVCSKDPK 174
+ K + + E EY K + AF+ I +GCN+FC++C++PYTRG S+
Sbjct: 121 KNKKSVTNIDFPLTEKFNFIEYPRKPKVTAFVSIMEGCNKFCSFCIVPYTRGHEISRPVD 180
Query: 175 KVLDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKVT-LVDLLEEIEKIDGIERVRIGSI 232
+L EI +L+ G KEI L G + SY G + + +LL + IDGI+R+R +
Sbjct: 181 DILLEISTLSSRGVKEIHLLGQNVNSYKGKTFNGDICKFSNLLRLVASIDGIDRIRFTTS 240
Query: 233 DPTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIED 292
+P FT+D++ + K+ HL +QSG + L+ M R +T EY+ I+N + +
Sbjct: 241 NPFEFTDDIIEIYAEIPKIVSFLHLPVQSGSNRILQLMKRMHTIDEYKTIINKILKLRPN 300
Query: 293 VSITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKD 352
+ I++D I+GFPG +K + + ++ F YSPR GT A +++ V +K
Sbjct: 301 IQISSDFIIGFPGETLIDFEQTLQLIKDLNIDMSYSFIYSPRPGTPASELQDNVTLCEKQ 360
Query: 353 ERSKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVS 412
+R L L R N + +K +G + VL E + G T N VN K + N+
Sbjct: 361 KRLHILQTLIRNNTTMWNQKMLGSIQSVLVEGRSQKNPKELFGRTENNRIVNFKGNQNMI 420
Query: 413 GKILNVKINSFEGEVAEG 430
G+ +N+KI G
Sbjct: 421 GEFINLKITKINPNSLRG 438
>MIAB_BURXL RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 457
Score = 207 bits (526), Expect = 2e-55
Identities = 142/446 (31%), Positives = 212/446 (47%), Gaps = 15/446 (3%)
Query: 2 KVAFATLGCRVNTYESEAMTEKF-VREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
KV T GC++N Y+S+ M + EG D E ADV + NTCSV +K +
Sbjct: 4 KVYVKTFGCQMNEYDSDKMVDVLGAAEGLVKTDTPEDADVILFNTCSVREKAQEKVFSDL 63
Query: 61 GRARR---QNPEAIIAVVGCYA-QIAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR R NP II V GC A Q + V+ VD+V G + + ++K R+ G+
Sbjct: 64 GRVRELKEANPNLIIGVGGCVASQEGASIVARAPYVDLVFGPQTLHRLPQMIDKRRESGR 123
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
QV + K F+ L + + AF+ I +GC+++C+YC++PYTRG S+ V
Sbjct: 124 AQVDISFPEIEK-FDHLPPARVDGPS-AFVSIMEGCSKYCSYCVVPYTRGEEVSRPLDDV 181
Query: 177 LDEIRSLAEHGFKEIILSGIHTASYGVDLD----EKVTLVDLLEEIEKIDGIERVRIGSI 232
L EI LA+ G +E+ L G + +Y L E L+E + I GIER+R +
Sbjct: 182 LTEIAGLADQGVREVTLLGQNVNAYRAGLTLGSTEIADFAQLIEYVADIPGIERIRYTTS 241
Query: 233 DPTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIED 292
P FT+ ++ + KL H HL +Q G D L M R YT EY+ ++ LR D
Sbjct: 242 HPKEFTQRLIDTYAKVPKLVSHLHLPVQHGSDRILMAMKRGYTVLEYKSVIRKLRAIRPD 301
Query: 293 VSITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKD 352
+S++TD+IVGFPG + + + F YSPR GT A N+ + K
Sbjct: 302 LSLSTDLIVGFPGETEEDFDKMMTLVHEMKYDTSFSFIYSPRPGTPAANLHDDTPREVKL 361
Query: 353 ERSKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKA----D 408
R + L N + ++ +G++ +L E G T N VN A
Sbjct: 362 RRLQHLQATIEENVQRISDSMVGKIERILVERPARKDPNELAGRTENNRVVNFPAPVASH 421
Query: 409 CNVSGKILNVKINSFEGEVAEGEIVL 434
+ G++++VKI GE+VL
Sbjct: 422 ARLIGQMVDVKIVKAYPHSLRGELVL 447
>MIAB_RICAH RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 445
Score = 206 bits (525), Expect = 2e-55
Identities = 149/444 (33%), Positives = 241/444 (54%), Gaps = 31/444 (6%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIGRARRQ 66
T GC++N Y+S M + GYE + E ADV ++NTC + +K+ +GR ++
Sbjct: 9 TYGCQMNVYDSVKMQDLLYPFGYEPTENIEEADVIILNTCHIREKAAEKTYSELGRIKKL 68
Query: 67 NP--------EAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGKQ 117
AII V GC AQ E+ + VD+V+G ++ + ++K K
Sbjct: 69 QDTRKKQGLNSAIIVVAGCVAQAEGEEIFTRTPYVDIVVGPQSYYNLPELISKIVRHAKH 128
Query: 118 QVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVL 177
+ + V + K F++L + Y +F+ +Q+GC++FC +C++PYTRG+ S++ ++V
Sbjct: 129 LIDLDFVEEAK-FDNLPEQLYPQGASSFISVQEGCDKFCTFCVVPYTRGAEFSRNVEQVY 187
Query: 178 DEIRSLAEHGFKEIILSGIHTASYGV--DLDEKVTLVDLLEEIEKIDGIERVRIGSIDPT 235
E + G KEI+L G + +Y D+ +L DLL+ + +I +ER+R + P
Sbjct: 188 REALQVVSGGAKEIMLLGQNVNAYNWKGSADKIFSLADLLKHLAQIPNLERLRYMTSHPI 247
Query: 236 FFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSI 295
T+D+++ KL P HL +QSG + LK MNR++ + Y DI+N LR+ D+ +
Sbjct: 248 DMTDDLIQLHGTEPKLMPFLHLPVQSGSNKILKAMNRKHDREYYFDIINRLREARPDIVL 307
Query: 296 TTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERS 355
++D IVGFPG ++R+ + + FKYSPR GT +Q+ + K ER
Sbjct: 308 SSDFIVGFPGXXXXXXXXXXXXVRRVKYGQCYSFKYSPRPGTPGATRTDQIPEHIKSERL 367
Query: 356 KKLIELNRINEKAFAEKYIGEVIDVLFE-----EEVELGSGVY--TGYTRNYIKVNAKAD 408
L + + A E +G I VLF+ ++ +G +Y + Y +N K
Sbjct: 368 TILQKELMDQQLACNESCVGSTIKVLFDRSGKFDDQIIGKTIYMQSVYIQNPNK------ 421
Query: 409 CNVSGKILNVKI-----NSFEGEV 427
++ GKI++VKI NS GE+
Sbjct: 422 -SLLGKIIDVKITKASLNSLTGEI 444
>MIAB_ROSDO RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 441
Score = 206 bits (524), Expect = 3e-55
Identities = 129/390 (33%), Positives = 201/390 (51%), Gaps = 10/390 (2%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIG 61
K+ T GC++N Y+SE M+E V EGY E AD+ ++NTC + +K +G
Sbjct: 6 KLFIKTYGCQMNVYDSERMSEALVAEGYVETKTAEDADMILLNTCHIREKAAEKVYSELG 65
Query: 62 RARR---QNPEAIIAVVGCYAQIAPTEVSGIE-GVDVVLGSRNKGEIVYYVNKARDEGKQ 117
R + NP+ + V GC AQ E+ + VD+V+G ++ + + R EG +
Sbjct: 66 RLKSLKADNPDLKLGVAGCVAQAEGEEIMRRQPAVDLVVGPQSYHRLPQMEARLR-EGHK 124
Query: 118 QVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVL 177
+ ++K E AFL +Q+GC++FCA+C++PYTRG+ S+ +VL
Sbjct: 125 ALDTDFPPEDKFEELXXXXXXXXXXSAFLTVQEGCDKFCAFCVVPYTRGAEVSRPVTRVL 184
Query: 178 DEIRSLAEHGFKEIILSGIHTASY---GVDLDEKVTLVDLLEEIEKIDGIERVRIGSIDP 234
DE R L E G +EI L G + +Y G D +EK TL L+ + IDG+ER+R + P
Sbjct: 185 DEARDLVERGVREITLLGQNVNAYHGAGADGNEK-TLAQLIWALNDIDGLERIRFTTSHP 243
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
+D++ KL P+ HL +QSG D LKRMNR++TA Y ++ +R D+
Sbjct: 244 NDMQDDLIEAHRDCPKLMPYLHLPVQSGSDRILKRMNRKHTADSYLRLIERIRVGRPDIL 303
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
++ D IVGFP ++ + + FKYS R GT A QVD + DER
Sbjct: 304 LSGDFIVGFPEETEADFQATLDLIEAVNYGYAYSFKYSTRPGTPAAE-RAQVDPVEADER 362
Query: 355 SKKLIELNRINEKAFAEKYIGEVIDVLFEE 384
+++ L ++ + +G + VL E+
Sbjct: 363 LQRIQALITRQQQDIQQSMVGRDVSVLIEK 392
>MIAB_RICCK RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 446
Score = 206 bits (524), Expect = 3e-55
Identities = 146/444 (32%), Positives = 238/444 (53%), Gaps = 16/444 (3%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIG 61
K+ T GC++N Y+S M + GYE + + ADV ++NTC + +K +G
Sbjct: 4 KLYIKTYGCQMNVYDSVKMQDLLYPFGYESTENIKEADVIILNTCHIREKAAEKIYSELG 63
Query: 62 RARRQNP--------EAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKAR 112
R ++ AII V GC AQ E+ + VD+V+G ++ + ++K
Sbjct: 64 RIKKLQDTRKKHGLSSAIIVVAGCVAQAEGEEIFTRTPYVDIVVGPQSYYNLPELISKVV 123
Query: 113 DEGKQQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKD 172
K + + V + K F+ L + Y AF+ +Q+GC++FC +C++PYTRG S++
Sbjct: 124 RHEKHLIDLDFVEEAK-FDQLPEQLYPQGASAFISVQEGCDKFCTFCVVPYTRGVEFSRN 182
Query: 173 PKKVLDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKV-TLVDLLEEIEKIDGIERVRIG 230
++V E + +G +EI+L G + +Y G +D+K+ +L DL+ + +I +ER+R
Sbjct: 183 VEQVYREALKVVSNGAREIMLLGQNVNAYHGKGVDDKIFSLADLIRYLAQIPNLERLRYT 242
Query: 231 SIDPTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKI 290
+ P T+D+++ KL P HL +QSG + LK MNR++ + Y +I+N LR+
Sbjct: 243 TSHPIDMTDDLIKLHSIESKLMPFVHLPVQSGSNKILKAMNRKHDREYYFNIINRLREAR 302
Query: 291 EDVSITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNK 350
D+ +++D IVGFPG +++I + + FKYSPR GT +Q+ +
Sbjct: 303 PDIVLSSDFIVGFPGXXXXXXXXXXXXVRKIKYGQCYSFKYSPRPGTPGATRTDQIPEHI 362
Query: 351 KDERSKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVY--TGYTRNYIKVNAKAD 408
K ER L + + AF E +G + VLF+ + + T Y ++ N D
Sbjct: 363 KSERLTILQKELSSQQLAFNESCVGSTMKVLFDRSGKFDGQIIGKTPYMQSVYINNTNQD 422
Query: 409 CNVSGKILNVKINSFEGEVAEGEI 432
+ KI++VKI GEI
Sbjct: 423 --LLCKIIDVKITKATSNSLTGEI 444
>MIAB_FERNB RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 430
Score = 206 bits (524), Expect = 3e-55
Identities = 133/433 (30%), Positives = 225/433 (51%), Gaps = 9/433 (2%)
Query: 1 MKVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
MKV T GC++N +SE + + EG E+ D ADV ++NTC+V ++K
Sbjct: 1 MKVHIFTYGCQMNENDSEIVKQLLKDEGIELTDDENDADVVILNTCAVRKKSEEKVYS-- 58
Query: 61 GRARRQNPEAIIAVVGCYAQIAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVK 120
+GC A+ + GV V+G+R ++ V A++ G +Q+
Sbjct: 59 HXXXXXXXXXXXXXMGCVAEKEKENLFK-RGVSFVIGTRALTKVPDAVLNAKN-GNKQIY 116
Query: 121 VGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDEI 180
+ L E K+E ++ A++ I GC+RFC YC++PYTRG S+ +VL E+
Sbjct: 117 LEDTLDE--IEYHKVETRSSNHHAWVTIIHGCDRFCTYCIVPYTRGREKSRPIDEVLKEV 174
Query: 181 RSLAEHGFKEIILSGIHTASYGVDLDEKVTLVDLLEEIEKIDGIERVRIGSIDPTFFTED 240
SL++ G+KE G + +YG DL + +L LL+ +ID ++R+ + PT F+ +
Sbjct: 175 ESLSQRGYKEFTFLGQNVDAYGKDLRDGTSLAKLLKLASQIDNVKRLWFLTSYPTDFSLE 234
Query: 241 VVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITTDVI 300
+ + +L +K+ HL +Q G D LK MNRRYT +EY D++N +R + D SI++D+I
Sbjct: 235 IPKVMLESEKVARSIHLPVQHGSDKILKAMNRRYTRQEYIDLINEIRKIVPDASISSDII 294
Query: 301 VGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRA-ENMENQVDGNKKDERSKKLI 359
VGFPG +K + + ++ YSPR+GT A + +++ V K R L+
Sbjct: 295 VGFPGENDEDFEATVELVKLLKFERLNLAVYSPREGTVAWKYLKDDVPYQVKVRRMSYLL 354
Query: 360 ELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSGKILNVK 419
L + K + Y+G+ ++V+ E + + +G++ G ++ +A+ GK + VK
Sbjct: 355 NLQKTINKELNKSYLGKEVEVIVEAQAK--NGLFYGRDIRNKIISFEANPESIGKNVIVK 412
Query: 420 INSFEGEVAEGEI 432
+N GEI
Sbjct: 413 VNKISAGPLYGEI 425
>MIAB_SALTO RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 499
Score = 206 bits (523), Expect = 4e-55
Identities = 137/434 (31%), Positives = 214/434 (49%), Gaps = 21/434 (4%)
Query: 7 TLGCRVNTYESEAMTEKFVREGY-EVVDFNEMADVYVVNTCSVTNMGDKKSRQIIGRARR 65
T GC++N ++SE + + GY ++ DV V NTC+V D + +G+ R
Sbjct: 16 TYGCQMNVHDSERIAGLLEQAGYLRAGAADDTPDVVVFNTCAVRENADNRLYGNLGQLRP 75
Query: 66 ---QNPEAIIAVVGCYAQIAPTE-VSGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVKV 121
Q+PE IAV GC AQ +E V VDVV G+ N G + + +AR +V++
Sbjct: 76 RKDQHPEMQIAVGGCLAQKDRSEIVRRAPWVDVVFGTHNIGSLPVLLERARHNAAAEVEI 135
Query: 122 GAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDEIR 181
L+ VF + ++ I GCN C +C++P RG + P ++L E+R
Sbjct: 136 LESLE--VFPSTLPTRRESTYAGWVSISVGCNNTCTFCIVPALRGKEKDRRPGEILSEVR 193
Query: 182 SLAEHGFKEIILSGIHTASYGVDLDEKVTLVDLLEEIEKIDGIERVRIGSIDPTFFTEDV 241
SL + G E+ L G + SYG++ ++ LL IDG+ERVR S P FT+DV
Sbjct: 194 SLVDEGVLEVTLLGQNVNSYGIEFGDRYAFGKLLRACGDIDGLERVRFTSPHPKDFTDDV 253
Query: 242 VRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITTDVIV 301
+ + +CP H+ LQSG D L+ M R Y ++ Y I+ +R + D +ITTD+IV
Sbjct: 254 IAAMAETPNVCPSLHMPLQSGSDDMLRAMRRSYRSERYLGIIEKVRAAMPDAAITTDIIV 313
Query: 302 GFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSKKLIEL 361
GFPG ++ S F+YS R GT A M +Q+ ER ++LI+
Sbjct: 314 GFPGETEADFERTLDVVRAARFSSAFTFQYSKRPGTPAATMADQLPKAVVQERYERLIDC 373
Query: 362 NRINEKAFAE--KYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSGKILNVK 419
+ E +AE + +G+ ++VL V +G G R + + G++++
Sbjct: 374 --VEEITWAENRRLLGKTVEVL----VAVGEG------RKDERTGRLSGRARDGRLVHFD 421
Query: 420 INSFEGEVAEGEIV 433
S G+V G+IV
Sbjct: 422 AGSLAGQVRPGDIV 435
>MIAB_BRASB RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 466
Score = 206 bits (523), Expect = 4e-55
Identities = 137/443 (30%), Positives = 220/443 (49%), Gaps = 15/443 (3%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIG 61
K+ + GC++N Y+++ M + EG+ AD+ ++NTC + +K +G
Sbjct: 6 KLHIKSYGCQMNVYDAQRMVDTLGAEGFVETAEAGDADLVILNTCHIREKASEKVYSELG 65
Query: 62 RARRQNPEAI-------IAVVGCYAQIAPTE-VSGIEGVDVVLGSRNKGEIVYYVNKARD 113
R R EA IAV GC AQ E V+ VDVV+G ++ + + +A
Sbjct: 66 RLRVAKEEAARQGRAMQIAVAGCVAQAEGAEIVTRAPTVDVVVGPQSYHHLPELLARA-G 124
Query: 114 EGKQQVKVGAVLKNKVFEDLKIEEYNNKTR---AFLKIQDGCNRFCAYCLIPYTRGSVCS 170
G+ ++ ++K + + R AF+ +Q+GC++FC +C++PYTRGS S
Sbjct: 125 RGEPAIETEFPAEDKFGFLARPSREAIRARGVSAFVTVQEGCDKFCTFCVVPYTRGSEMS 184
Query: 171 KDPKKVLDEIRSLAEHGFKEIILSGIHTASYGVDLDEKVT--LVDLLEEIEKIDGIERVR 228
+ ++LDE+ L E+G +EI L G + +Y + + T L LL I +I G+ R+R
Sbjct: 185 RPVARILDEVTRLTENGVREITLIGQNVNAYHGEAPDGSTWTLGRLLYGIAEIPGVARIR 244
Query: 229 IGSIDPTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRD 288
+ P + ++ L + P HL +QSG D L MNR++ A +Y +V+ R
Sbjct: 245 YSTSHPNDVDDSLIAAHRDLASVMPFVHLPVQSGSDRILGLMNRKHGASDYRKVVDRFRA 304
Query: 289 KIEDVSITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDG 348
D++ ++D IVGFPG + +I + + FKYSPR GT A +M+ V
Sbjct: 305 ARPDIAFSSDFIVGFPGETEEDFRATLALIAQIGYAAAYSFKYSPRPGTPAADMQEMVSA 364
Query: 349 NKKDERSKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKAD 408
+ DER ++L L + AF + IG V+DVLFE G G T + A
Sbjct: 365 TEMDERLERLQSLIDSQQAAFNKAAIGTVVDVLFERAAR-KPGQLVGRTAYLQPAHVMAP 423
Query: 409 CNVSGKILNVKINSFEGEVAEGE 431
N+ G++L V+I+S E GE
Sbjct: 424 DNIIGQVLPVRIDSLERYSLLGE 446
>MIAB_SINMW RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 472
Score = 205 bits (522), Expect = 5e-55
Identities = 135/432 (31%), Positives = 219/432 (50%), Gaps = 15/432 (3%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIG 61
KV T GC++N Y+S+ M++ R+GY D E AD ++NTC + +K +G
Sbjct: 23 KVFVKTYGCQMNVYDSDRMSDALSRDGYVATDVLEEADFVLLNTCHIREKAAEKVYSELG 82
Query: 62 RAR-------RQNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARD 113
R R R+ E +I V GC AQ E+ VD+V+G + + + +AR
Sbjct: 83 RLRDLKKAKAREGREMLIGVAGCVAQAEGKEILRRAPAVDLVIGPQTYHRLPEALRRART 142
Query: 114 EGKQQVKVGAVLKNKVFEDLKIEEYNNKTR---AFLKIQDGCNRFCAYCLIPYTRGSVCS 170
G++ V+ +++K ++ + R AFL +Q+GC++FC +C++PYTRGS S
Sbjct: 143 -GERIVETDYAIEDKFVHLPAPDKAKTRARGVTAFLTVQEGCDKFCTFCVVPYTRGSEVS 201
Query: 171 KDPKKVLDEIRSLAEHGFKEIILSGIHTASY--GVDLDEKVTLVDLLEEIEKIDGIERVR 228
+ +++ E L E G +EI L G + ++ + L DLL + +I+G+ R+R
Sbjct: 202 RPVAQIVAEAEKLVEGGVREITLLGQNVNAWHGAGPHGREWGLGDLLRRLGEIEGLARLR 261
Query: 229 IGSIDPTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRD 288
+ P + ++ ++ KL P+ HL +QSG D LK MNRR+TA EY +V +R
Sbjct: 262 YTTSHPRDMDDSLIEAHRSMAKLMPYLHLPVQSGSDRILKAMNRRHTAAEYLALVARIRA 321
Query: 289 KIEDVSITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDG 348
D++++ D IVGFPG + + ++ FKYS R GT +E QV
Sbjct: 322 VQPDLALSGDFIVGFPGETDEDFEDTLRLVDAVNYAQAFSFKYSTRPGTPGAELEQQVPE 381
Query: 349 NKKDERSKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKAD 408
+ K +R + L L ++ FAE +G ID+L E+ + G G + VN A
Sbjct: 382 DVKAKRLEILQALLIKQQRGFAEACVGREIDLLLEKPGRM-PGQLVGRSPWLQPVNVDAK 440
Query: 409 CNVSGKILNVKI 420
+ G I+ V+I
Sbjct: 441 ASQIGDIIRVRI 452
>MIAB_PROMS RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 464
Score = 204 bits (520), Expect = 9e-55
Identities = 135/444 (30%), Positives = 216/444 (48%), Gaps = 20/444 (4%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIGRA--- 63
T GC++N +SE M + GY D AD+ + NTC++ + ++K +GR
Sbjct: 25 TFGCQMNKADSERMAGTLEKMGYTRADNELNADLVLYNTCTIRDNAEQKVYSFLGRQAKR 84
Query: 64 RRQNPEAIIAVVGCYAQI-APTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVKVG 122
+ + P + V GC AQ + + + +D+V+G ++ + + K D G Q V
Sbjct: 85 KHKTPSLKLVVAGCLAQQEGESLLRRVPELDLVMGPQHVNNLENLLGKV-DLGNQ---VA 140
Query: 123 AVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDEIRS 182
A +N + ED+ + ++ I GCN C+YC++P RG S+ P + EI+
Sbjct: 141 ATEENFISEDITSARRESSICGWVNIIYGCNERCSYCVVPSVRGKEQSRYPNAIKSEIQK 200
Query: 183 LAEHGFKEIILSGIHTASYGVDLD-------EKVTLVDLLEEIEKIDGIERVRIGSIDPT 235
LA FKEI L G + +YG DL ++ TL DLL I + GI R+R + P
Sbjct: 201 LAGDNFKEITLLGQNIDAYGRDLPGTTKEGRKENTLTDLLYYIHDVKGIRRIRFATSHPR 260
Query: 236 FFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSI 295
+F++ +++ L K+C HFH+ QSG D LK+M+R YT K+Y++I+ +R + D SI
Sbjct: 261 YFSKRLIQACYELDKVCEHFHIPFQSGNDEILKQMSRGYTIKKYKNIIENIRSLMPDASI 320
Query: 296 TTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERS 355
T D IV FPG + I + + YSPR T A NQ+ K R
Sbjct: 321 TADAIVAFPGETEKQYQDTLKLITEIGFDQVNTAAYSPRPNTPAAVWSNQLSEEVKKARL 380
Query: 356 KKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTR----NYIKVNAKADCNV 411
+++ +L ++ ++YI + VL E S G TR ++++ D N
Sbjct: 381 QEINDLVEKTARSRNKRYINNIESVLIEGLNPKNSSQIMGRTRTNRLTFVEIPKNIDFNF 440
Query: 412 S-GKILNVKINSFEGEVAEGEIVL 434
S G ++V+IN GE+ L
Sbjct: 441 SLGDEIDVRINETRPFSLTGELYL 464
>MIAB_COPPD RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 426
Score = 204 bits (520), Expect = 9e-55
Identities = 136/435 (31%), Positives = 222/435 (51%), Gaps = 16/435 (3%)
Query: 1 MKVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
MK T GC++N +SE ++ G+E +D+ V+NTCSV ++++ I
Sbjct: 1 MKYYIFTYGCQMNKNDSEMVSGILKSSGWEEAKNVVDSDLVVINTCSVRLHAEERAIGTI 60
Query: 61 GRARRQNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQV 119
+ +GC +++ E+ S V VLG + I+ +N G++++
Sbjct: 61 --SAXXXXXXXXXXMGCMSEVRGNEIMSRFPHVQAVLGPSYEAHILDVLN-----GERRI 113
Query: 120 KVGAVLKNKV-FEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLD 178
VG KV FE K ++ I GC+ FC YC++P+TRG V S+DP+ +L+
Sbjct: 114 LVG---DEKVDFEKYSSANRKEKHSVYVSIMKGCDDFCTYCIVPFTRGRVQSRDPESILE 170
Query: 179 EIRSLAEHGFKEIILSGIHTASYGVDLDEKVTLVDLLEEIEKIDGIERVRIGSIDPTFFT 238
E+R ++G EI L G + YG DL V L+E + IDG+ R+R S P F
Sbjct: 171 EVRVCVDNGAVEITLLGQNVNDYGKDLSGW-DFVSLVERVATIDGVRRIRFMSPHPANFK 229
Query: 239 EDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITTD 298
+D + R+ L ++ P++HL LQSG D L+RMNR+YT E+ ++V +R+ + +V+I TD
Sbjct: 230 KDDITRLANLPQVAPYYHLPLQSGDDEILRRMNRKYTTGEFAELVGFIRESVPNVAIGTD 289
Query: 299 VIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQ-VDGNKKDERSKK 357
+IVGFPG L+++ ++ YSPR GT A E V R +
Sbjct: 290 LIVGFPGESDEHFQNTFKFLEKMQFDVVYMAIYSPRPGTAAARQETSFVPAEVAKARYDE 349
Query: 358 LIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVS-GKIL 416
L+ L + ++Y+G + +VL + E + +G + G T V + +VS G+ +
Sbjct: 350 LLRLQEKISYSINQRYVGTLQEVLIDRE-DKTTGKFIGRTPTNKTVVFTSIRHVSPGEFV 408
Query: 417 NVKINSFEGEVAEGE 431
+V+IN + V GE
Sbjct: 409 DVRINEAKSWVLYGE 423
>MIAB_THEFY RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 494
Score = 204 bits (519), Expect = 1e-54
Identities = 129/383 (33%), Positives = 197/383 (51%), Gaps = 8/383 (2%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIGRARR- 65
T GC++N ++SE ++ GY+ DV V NTC+V D + +G+ R
Sbjct: 11 TYGCQMNVHDSERLSGLLEAAGYQRAAEGTTPDVVVFNTCAVRENADNRLYGNLGQLRPV 70
Query: 66 --QNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVKVG 122
NP+ IAV GC AQ E+ VDVV G+ N G + + +AR + + QV++
Sbjct: 71 KDANPDMQIAVGGCLAQKDRGEIIRRAPWVDVVFGTHNIGSLPALLERARVQREAQVEIV 130
Query: 123 AVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDEIRS 182
L+ F + A++ I GCN C +C++P RG + P +L EIR+
Sbjct: 131 ESLER--FPSTLPTRRESVYAAWVAISVGCNNTCTFCIVPALRGKEKDRRPGDILAEIRA 188
Query: 183 LAEHGFKEIILSGIHTASYGVDLDEKVTLVDLLEEIEKIDGIERVRIGSIDPTFFTEDVV 242
+ G E+ L G + +YG + ++ LL +I+G+ERVR S P FT+DV+
Sbjct: 189 VVAEGAIEVTLLGQNVNAYGSEFGDRQAFSKLLRACGEIEGLERVRFTSPHPRDFTDDVI 248
Query: 243 RRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITTDVIVG 302
+ + P H+ LQSG D L+ M R Y A+ + IV +R+ I D +ITTDVIVG
Sbjct: 249 AAMAETPNVMPQLHMPLQSGSDRILRAMRRSYRAERFLRIVEKVREAIPDAAITTDVIVG 308
Query: 303 FPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSKKLIEL- 361
FPG ++ + + F+YS R GT A +M+NQV ER ++L+EL
Sbjct: 309 FPGETEEDFAETLRVMEEVRFAHAFTFQYSKRPGTPAASMDNQVPREVVKERYQRLLELQ 368
Query: 362 NRINEKAFAEKYIGEVIDVLFEE 384
RI+E+ A K++G ++VL E
Sbjct: 369 ERISEEENA-KFVGREVEVLVAE 390
>MIAB_RHOP5 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 465
Score = 204 bits (519), Expect = 1e-54
Identities = 133/445 (29%), Positives = 223/445 (50%), Gaps = 15/445 (3%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIG 61
K+ + GC++N Y+++ M + EG+ + AD+ ++NTC + +K +G
Sbjct: 6 KLHIKSFGCQMNVYDAQRMVDALAPEGFVETQSADDADLVILNTCHIREKAAEKVYSELG 65
Query: 62 RARRQNPEAI-------IAVVGCYAQIAPTEVSGIE-GVDVVLGSRNKGEIVYYVNKARD 113
+ R +A IAV GC AQ E+ + VDVV+G ++ + + KAR
Sbjct: 66 KLRLLKQDAASHGRRFEIAVAGCVAQAEGAEIIRRQPAVDVVVGPQSYHHLPELLEKARR 125
Query: 114 EGKQ-QVKVGAVLKNKVFEDLKIEEYNNK-TRAFLKIQDGCNRFCAYCLIPYTRGSVCSK 171
+G+ + + K V + + + AF+ +Q+GC++FC++C++PYTRG+ S+
Sbjct: 126 DGRALETEFPIEDKFGVLPPPRPDAIRARGVSAFVTVQEGCDKFCSFCVVPYTRGAEMSR 185
Query: 172 DPKKVLDEIRSLAEHGFKEIILSGIHTASY---GVDLDEKVTLVDLLEEIEKIDGIERVR 228
K+LD+++ L ++G +EI L G + +Y G D D TL LL + + G+ R+R
Sbjct: 186 PVAKILDDVKRLIDNGVREITLIGQNVNAYHGEGPD-DRPWTLGALLRHLASVPGVARLR 244
Query: 229 IGSIDPTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRD 288
+ P ++++ L L P HL +QSG DA L MNRR++A +Y +++ R
Sbjct: 245 YSTSHPLDVDDELIAAHRELPGLMPFVHLPVQSGSDAILAAMNRRHSADDYRRVIDRFRQ 304
Query: 289 KIEDVSITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDG 348
++ ++D IVGFPG + +I + + FKYSPR GT A ++ V
Sbjct: 305 ADPSIAFSSDFIVGFPGETDRDFEATLALVTQIGYAGAYSFKYSPRPGTPAAELQEMVAP 364
Query: 349 NKKDERSKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKAD 408
D+R ++L L + AF IG +DVLFE G G T + A
Sbjct: 365 AVMDQRLEQLQGLIDSQQAAFNRASIGTTVDVLFERAAR-HPGQIVGRTAYLQPAHVMAA 423
Query: 409 CNVSGKILNVKINSFEGEVAEGEIV 433
++ G++L V I+S E GE+V
Sbjct: 424 DDIVGQVLPVTIHSLERYSLIGELV 448
>MIAB_PROMM RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 480
Score = 204 bits (519), Expect = 1e-54
Identities = 138/432 (31%), Positives = 207/432 (47%), Gaps = 20/432 (4%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIGRA--- 63
T GC++N +SE M GY AD+ + NTC++ + ++K +GR
Sbjct: 35 TFGCQMNKADSERMAGILEAMGYHEAPAELEADLVLYNTCTIRDNAEQKVYSYLGRQARR 94
Query: 64 RRQNPEAIIAVVGCYAQI-APTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVKVG 122
+R +P + V GC AQ + I +D+V+G ++ + + + D G+Q V
Sbjct: 95 KRTHPHLKLVVAGCVAQQEGEALLRRIPELDLVMGPQHANRLEALLTQV-DNGQQVV--- 150
Query: 123 AVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDEIRS 182
A N + EDL ++ A++ + GCN C YC++P RG S+ P+ + EI
Sbjct: 151 ATDDNHILEDLTTARRDSTICAWVNVIYGCNERCTYCVVPSVRGKEQSRSPEAIRLEIEG 210
Query: 183 LAEHGFKEIILSGIHTASYGVDLD-------EKVTLVDLLEEIEKIDGIERVRIGSIDPT 235
LA GF+EI L G + +YG DL + TL DLL I ++GIER+R + P
Sbjct: 211 LAARGFREITLLGQNIDAYGRDLPGITPEGRRQNTLTDLLHHIHDVEGIERIRFATSHPR 270
Query: 236 FFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSI 295
+FTE ++ L K+C HFH+ QSG + LK M R YT + Y IVN +R+ + D +I
Sbjct: 271 YFTERLIEACFDLPKVCEHFHIPFQSGDNDVLKAMARGYTVERYRRIVNRIRELMPDAAI 330
Query: 296 TTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERS 355
+TDVIV FPG L+ + + + YSPR T A NQ+ K ER
Sbjct: 331 STDVIVAFPGETDAQFQNTLNLLEEVGFDQVNTAAYSPRPNTPAATWSNQLPEAVKVERL 390
Query: 356 KKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTR----NYIKVNAKADCNV 411
K+L L +Y G+V VL E G TR + C
Sbjct: 391 KQLNALVERIALQRNSRYSGKVEQVLAEGINPKKPQQLMGRTRTNRLTFFATEGPQGCRY 450
Query: 412 S-GKILNVKINS 422
S G +++++INS
Sbjct: 451 SPGDLVDIQINS 462
>MIAB_PARL1 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 467
Score = 204 bits (519), Expect = 1e-54
Identities = 131/396 (33%), Positives = 200/396 (50%), Gaps = 15/396 (3%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIG 61
K+ T GC++N Y+S M + GY VD E AD+ ++NTC + +K +G
Sbjct: 16 KIFVKTYGCQMNVYDSARMVDVMAPSGYTEVDAPEDADIVILNTCHIREKAAEKVYSELG 75
Query: 62 RARR-------QNPEAIIAVVGCYAQIAPTEVSG-IEGVDVVLGSRNKGEIVYYVNKARD 113
R R + E +I V GC AQ E+ VD+VLG + + YV + +
Sbjct: 76 RLRELKKEKSGRGEELLIGVAGCVAQAEGEEMRRRAPVVDLVLGPQTYHRLPEYVARLAN 135
Query: 114 EGKQQVKVGAVLKNKVFEDLKIEEYNNK----TRAFLKIQDGCNRFCAYCLIPYTRGSVC 169
G V+ + +K F L + E AFL IQ+GC++FC +C++PYTRGS
Sbjct: 136 GGPGIVETEFPVDDK-FASLPMAEKRKTLARGATAFLTIQEGCDKFCTFCVVPYTRGSEF 194
Query: 170 SKDPKKVLDEIRSLAEHGFKEIILSGIHT-ASYGVD-LDEKVTLVDLLEEIEKIDGIERV 227
S+ ++LDE RSL + G +EI L G + A +G D TL L+ + +I+G+ R+
Sbjct: 195 SRPVARILDEARSLVDAGVREITLLGQNVNAWHGEDQAGRPATLGYLIRALAEIEGLARL 254
Query: 228 RIGSIDPTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLR 287
R + P E+++ + L P+ HL +QSG D L MNRR+ A Y I+ +R
Sbjct: 255 RYTTSHPRDMDEELIAAHRDVPALMPYLHLPVQSGSDRILAAMNRRHDADSYHRIIGRIR 314
Query: 288 DKIEDVSITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVD 347
D+++++D IVGFPG ++ + ++ + FKYSPR GT A E+QV
Sbjct: 315 AAKPDIALSSDFIVGFPGETEADFEATLDLIRTVGFAQAYSFKYSPRPGTPAATEEDQVP 374
Query: 348 GNKKDERSKKLIELNRINEKAFAEKYIGEVIDVLFE 383
K ER ++L L + AF G + VLF+
Sbjct: 375 EEVKSERLQRLQALLGEQQLAFNAGCAGRTMPVLFD 410
>MIAB_EHRCR RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 442
Score = 204 bits (519), Expect = 1e-54
Identities = 122/383 (31%), Positives = 200/383 (52%), Gaps = 7/383 (1%)
Query: 9 GCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIGRARR--Q 66
GC++N Y+S M G+ VV+ A++ ++NTC + +K +G+ R+ +
Sbjct: 10 GCQMNVYDSLIMENIIKPLGFTVVNEPSEANIVILNTCHIREKASEKLYSELGKMRKIQE 69
Query: 67 NPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVKVGAVL 125
+ I V GC AQ ++ + VD+V+G ++ + + KAR KQ + + +
Sbjct: 70 TKDLTIVVAGCVAQAEGEQIFARAPFVDIVVGPQSIHTLPELIIKARRMKKQVINIDFPI 129
Query: 126 KNKVFEDLKIEEY--NNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDEIRSL 183
+K F+ + +EEY N + AF+ +Q+GCN+FC +C++PYTRG S+ + + +E L
Sbjct: 130 ISK-FDAIPVEEYTKNQEVSAFISVQEGCNKFCTFCVVPYTRGEEYSRTVEAIFNEALVL 188
Query: 184 AEHGFKEIILSGIHTASY-GVDLDEKVTLVDLLEEIEKIDGIERVRIGSIDPTFFTEDVV 242
++ G KEI L G + +Y G + L L++ + KI IER+R + P + +
Sbjct: 189 SDSGVKEITLIGQNVNAYHGTYKGCEWDLGKLIQYLAKIPNIERIRYTTSHPRDMHQSLY 248
Query: 243 RRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITTDVIVG 302
+ KL P HL +QSG D LK+MNR++TA+EY DI+N LR + D++ ++D IVG
Sbjct: 249 EAHRSETKLMPFVHLPIQSGSDRILKKMNRKHTAEEYIDIINNLRKQRPDIAFSSDFIVG 308
Query: 303 FPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSKKLIELN 362
FPG ++ + S+ + FKYSPR GT + NQ+ K +R
Sbjct: 309 FPGETEEDFEHTMKLVQEVNFSQAYSFKYSPRPGTPSAEYPNQIPEEIKSQRIFXXXXXX 368
Query: 363 RINEKAFAEKYIGEVIDVLFEEE 385
AF IG+ VLF +
Sbjct: 369 XXXXXAFNRNMIGQTCSVLFNNK 391
>MIAB_BURS3 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 457
Score = 204 bits (519), Expect = 1e-54
Identities = 141/446 (31%), Positives = 213/446 (47%), Gaps = 15/446 (3%)
Query: 2 KVAFATLGCRVNTYESEAMTEKF-VREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
KV T GC++N Y+S+ M + EG E D E AD+ + NTCSV +K +
Sbjct: 4 KVYVKTFGCQMNEYDSDKMVDVLNAAEGLEKTDTPEDADIILFNTCSVREKAQEKVFSDL 63
Query: 61 GRARR---QNPEAIIAVVGCYA-QIAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR R P +I V GC A Q + VS VD+V G + + ++ R+ G+
Sbjct: 64 GRVRELKEAKPGLLIGVGGCVASQEGASIVSRAPYVDLVFGPQTLHRLPQMIDARRESGR 123
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
QV + K F+ L + AF+ I +GC+++C+YC++PYTRG S+ V
Sbjct: 124 AQVDISFPEIEK-FDHLPPARVEGPS-AFVSIMEGCSKYCSYCVVPYTRGDEVSRPLDDV 181
Query: 177 LDEIRSLAEHGFKEIILSGIHTASYGVDL----DEKVTLVDLLEEIEKIDGIERVRIGSI 232
L E+ LA+ G +E+ L G + +Y L E L+E + I GIER+R +
Sbjct: 182 LTEVAGLADQGVREVTLLGQNVNAYRGALAAGSSEIADFATLIEYVADIPGIERIRYTTS 241
Query: 233 DPTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIED 292
P FT+ ++ + KL H HL +Q G D L M R YT EY+ ++ LR D
Sbjct: 242 HPKEFTQRLIDTYAKVPKLVNHLHLPVQHGSDRILMAMKRGYTVLEYKSVIRKLRAIRPD 301
Query: 293 VSITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKD 352
+S++TD+IVGFPG + ++ + F YSPR GT A N+ + + K
Sbjct: 302 LSLSTDMIVGFPGETEDDFDKMMALVHEMSYDTSFSFIYSPRPGTPAANLHDDTPRDVKL 361
Query: 353 ERSKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADC--- 409
+R + L N ++ +G+V +L E G T N VN A
Sbjct: 362 KRLQHLQATIEENVARISQSMVGKVERILVEGPSRKDPNELAGRTENNRVVNFPAPLASH 421
Query: 410 -NVSGKILNVKINSFEGEVAEGEIVL 434
+ G++++VKIN GE+VL
Sbjct: 422 PRLIGQMIDVKINHAYPHSLRGELVL 447
>MIAB_BACSU RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 509
Score = 204 bits (519), Expect = 1e-54
Identities = 132/440 (30%), Positives = 220/440 (50%), Gaps = 12/440 (2%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIG 61
K T GC++N +++E M F+ GYE + + A+V ++NTC++ + K +G
Sbjct: 67 KFYIRTYGCQMNEHDTEVMAGIFMALGYEATNSVDDANVILLNTCAIRENAENKVFGELG 126
Query: 62 RAR---RQNPEAIIAVVGCYAQ---IAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEG 115
+ + NP+ I+ V GC +Q + + VD++ G+ N + +++A
Sbjct: 127 HLKALKKNNPDLILGVCGCMSQEESVVNRILKKHPFVDMIFGTHNIHRLPELLSEAYLSK 186
Query: 116 KQQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKK 175
+ V+V + + V E+L + N K + ++ I GC++FC YC++PYTRG S+ P+
Sbjct: 187 EMVVEVWSK-EGDVIENLP-KVRNGKIKGWVNIMYGCDKFCTYCIVPYTRGKERSRRPED 244
Query: 176 VLDEIRSLAEHGFKEIILSGIHTASYGVDLDEKVT-LVDLLEEIEKIDGIERVRIGSIDP 234
++ E+R LA G+KEI L G + +YG D ++ L DL++E+ KID I R+R + P
Sbjct: 245 IIQEVRRLASEGYKEITLLGQNVNAYGKDFEDMTYGLGDLMDELRKID-IPRIRFTTSHP 303
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
F + ++ + L H HL +QSG LK M R+Y + Y ++V +++ + + S
Sbjct: 304 RDFDDRLIEVLAKGGNLLDHIHLPVQSGSSEVLKLMARKYDRERYMELVRKIKEAMPNAS 363
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
+TTD+IVGFP + + + F YSPR+GT A M++ V K ER
Sbjct: 364 LTTDIIVGFPNETDEQFEETLSLYREVEFDSAYTFIYSPREGTPAAKMKDNVPMRVKKER 423
Query: 355 SKKLIEL-NRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSG 413
++L L N I+ K + + GYT VN K G
Sbjct: 424 LQRLNALVNEISAKKMXXXXXXXXXXXXXXXS-KNNPDILAGYTEKSKLVNFKGPKEAIG 482
Query: 414 KILNVKINSFEGEVAEGEIV 433
KI+ VKI + +GE+V
Sbjct: 483 KIVRVKIQQAKTWSLDGEMV 502
>MIAB_BACLD RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 509
Score = 204 bits (519), Expect = 1e-54
Identities = 132/440 (30%), Positives = 223/440 (50%), Gaps = 12/440 (2%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIG 61
K T GC++N +++E M F+ GYE D E A+V ++NTC++ + K IG
Sbjct: 67 KFYIRTYGCQMNEHDTEVMAGIFMALGYEPTDSTEDANVILLNTCAIRENAENKVFGEIG 126
Query: 62 RAR---RQNPEAIIAVVGCYAQ---IAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEG 115
+ + NP+ I+ V GC +Q + + VD++ G+ N + +++A
Sbjct: 127 HLKALKKDNPDLILGVCGCMSQEESVVNRILKKHPFVDLIFGTHNIHRLPELLSEAYLSK 186
Query: 116 KQQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKK 175
+ ++V + + V E+L + K + ++ I GC++FC YC++PYTRG S+ P+
Sbjct: 187 EMVIEVWSK-EGDVIENLP-KVRTGKIKGWVNIMYGCDKFCTYCIVPYTRGKERSRRPED 244
Query: 176 VLDEIRSLAEHGFKEIILSGIHTASYGVDLDE-KVTLVDLLEEIEKIDGIERVRIGSIDP 234
++ E+R LA G+KEI L G + +YG D ++ + L L++E+ KID I R+R + P
Sbjct: 245 IIQEVRRLAAEGYKEITLLGQNVNAYGKDFEDMEYGLGHLMDELRKID-IPRIRFTTSHP 303
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
F + ++ + L H HL +QSG LK M R+Y + Y ++V ++ + + S
Sbjct: 304 RDFDDHLIEVLAKGGNLLDHIHLPVQSGSSDVLKLMARKYDRERYLELVAKIKKAMPNAS 363
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
+TTD+IVGFP + + + F YSPR+GT A M++ V K ER
Sbjct: 364 LTTDIIVGFPNETDEQFEETLSLYREVEFDSAYTFIYSPREGTPAAKMKDNVPMRVKKER 423
Query: 355 SKKLIE-LNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSG 413
++L E +N I+ K + + + GYTR VN K G
Sbjct: 424 LQRLNEVVNEISAKKM-KXXXXXXXXXXXXXXSKNNPDILAGYTRKNKLVNFKGPKEAIG 482
Query: 414 KILNVKINSFEGEVAEGEIV 433
+++NVKI+ + +GE+V
Sbjct: 483 QLVNVKIHQAKTWSLDGEMV 502
>MIAB_RICM5 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 450
Score = 204 bits (518), Expect = 2e-54
Identities = 149/445 (33%), Positives = 242/445 (54%), Gaps = 23/445 (5%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIG 61
K+ T GC++N Y+S M + GYE + E A V ++NTC + +K+ +G
Sbjct: 5 KLYIKTYGCQMNVYDSVKMQDLLYPFGYEPTENIEEAAVIILNTCHIREKAAEKTYSELG 64
Query: 62 RARRQNP--------EAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKAR 112
R ++ AII V GC AQ E+ + VD+V+G ++ + ++K
Sbjct: 65 RIKKLQDTRTKQGLSSAIIVVAGCVAQAEGEEIFTRTPYVDIVVGPQSYYNLPELISKVV 124
Query: 113 DEGKQQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKD 172
K + + V + K F+ L + Y T AF+ +Q+GC++FC +C++PYTRG+ S++
Sbjct: 125 RHEKHLIDLDFVEEAK-FDQLPEQLYPQGTSAFISVQEGCDKFCTFCVVPYTRGAEFSRN 183
Query: 173 PKKVLDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKV-TLVDLLEEIEKIDGIERVRIG 230
++V E + G KEI+L G + +Y G +K+ +L DLL+ + +I +ER+R
Sbjct: 184 VEQVYREALKVVSSGAKEIMLLGQNVNAYHGKGPADKIFSLADLLKHLAQIPNLERLRYI 243
Query: 231 SIDPTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKI 290
+ P +D+++ KL P HL +QSG + LK MNR++ + Y DI+N LR+
Sbjct: 244 TSHPIDMNDDLIKLHGTEPKLMPFLHLPVQSGSNKILKAMNRKHDREYYFDIINRLREAR 303
Query: 291 EDVSITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNK 350
+ +++D IVGFPG ++R+ + + FKYSPR GT +Q+ +
Sbjct: 304 PAIVLSSDFIVGFPGXXXXXXXXXXXXVRRVKYGQCYSFKYSPRPGTPGATKTDQIPEHI 363
Query: 351 KDERSKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVY--TGYTRN-YIKVNAKA 407
K ER L + + AF E +G + VLF+ + + T Y ++ YI+ K
Sbjct: 364 KSERLTILQQELMAQQLAFNESCVGSTMKVLFDRNGKCDDQIIGKTPYMQSVYIQNPNK- 422
Query: 408 DCNVSGKILNVKI-----NSFEGEV 427
++ GKI+++KI NS GE+
Sbjct: 423 --SLLGKIVDIKITKASLNSLTGEI 445
>MIAB_RHIEC RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 469
Score = 204 bits (518), Expect = 2e-54
Identities = 140/451 (31%), Positives = 221/451 (49%), Gaps = 24/451 (5%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIG 61
KV T GC++N Y+S M++ R+GYE + E AD+ ++NTC + +K +G
Sbjct: 23 KVFIKTYGCQMNVYDSTRMSDALARDGYEPTEDMEEADLVLLNTCHIREKAAEKVYSALG 82
Query: 62 RARRQNP-------EAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARD 113
R R E +I V GC AQ E+ VDVV+G + + + +A+
Sbjct: 83 RLREMKKKKAADGREMMIGVAGCVAQAEGEEILRRAPAVDVVIGPQTYHRLPEALRRAK- 141
Query: 114 EGKQQVKVGAVLKNKVFEDLKIEEYNNKTRA-----FLKIQDGCNRFCAYCLIPYTRGSV 168
EG++ V +++K FE L + E + K RA FL +Q+GC++FC +C++PYTRGS
Sbjct: 142 EGQRVVDTEYAIEDK-FEHLPVAE-SRKIRARGVTAFLTVQEGCDKFCTFCVVPYTRGSE 199
Query: 169 CSKDPKKVLDEIRSLAEHGFKEIILSGIHTASY--GVDLDEKVTLVDLLEEIEKIDGIER 226
S+ ++++E L E G +EI L G + ++ E +L DLL + +I G+ R
Sbjct: 200 VSRPVSQIVEEAEKLVEGGVREITLLGQNVNAWHGAGPRGEAWSLGDLLYRLAEIPGLAR 259
Query: 227 VRIGSIDPTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLL 286
+R + P + ++ L+ L P+ HL +QSG D LK MNRR+TA EY ++ +
Sbjct: 260 LRYTTSHPRDMDDRLIAAHRDLRALMPYLHLPVQSGSDRILKAMNRRHTAAEYLSLIERI 319
Query: 287 RDKIEDVSITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQV 346
R D++++ D I GFPG ++ + ++ FKYS R GT +++QV
Sbjct: 320 RAVRPDIALSGDFITGFPGETDKDFEDTLRLVEEVRYAQAFSFKYSTRPGTPGAELKDQV 379
Query: 347 DGNKKDERSKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAK 406
FAE IG+ ID+L E+ + G G + VN
Sbjct: 380 PEEIXXXXXXXXXXXXXXXXXEFAESCIGKEIDLLLEKPGRM-PGQLIGRSPWLQSVNVD 438
Query: 407 ADCNVSGKILNVKI-----NSFEGEVAEGEI 432
A + G I+ V+I NS E AE +
Sbjct: 439 AKASQIGDIIKVRITGTGTNSLFAERAEAAV 469
>MIAB_PROM3 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 480
Score = 204 bits (518), Expect = 2e-54
Identities = 139/435 (31%), Positives = 211/435 (48%), Gaps = 26/435 (5%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIGRA--- 63
T GC++N +SE M GY AD+ + NTC++ + ++K +GR
Sbjct: 35 TFGCQMNKADSERMAGILEAMGYHEAPAELEADLVLYNTCTIRDNAEQKVYSYLGRQARR 94
Query: 64 RRQNPEAIIAVVGCYAQI-APTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVKVG 122
+R +P + V GC AQ + I +D+V+G ++ + + + D G+Q V
Sbjct: 95 KRTHPHLKLVVAGCVAQQEGEALLRRIPELDLVMGPQHANRLEALLTQV-DNGQQVV--- 150
Query: 123 AVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDEIRS 182
A N + EDL ++ A++ + GCN C YC++P RG S+ P+ + EI
Sbjct: 151 ATDDNHILEDLTTARRDSTICAWVNVIYGCNERCTYCVVPSVRGKEQSRSPEAIRLEIEG 210
Query: 183 LAEHGFKEIILSGIHTASYGVDLD-------EKVTLVDLLEEIEKIDGIERVRIGSIDPT 235
LA GF+EI L G + +YG DL + TL DLL I ++GIER+R + P
Sbjct: 211 LAARGFREITLLGQNIDAYGRDLPGITPEGRRQNTLTDLLHHIHDVEGIERIRFATSHPR 270
Query: 236 FFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSI 295
+FTE ++ L K+C HFH+ QSG + LK M R YT + Y IVN +R+ + D +I
Sbjct: 271 YFTERLIEACFDLPKVCEHFHIPFQSGDNDVLKAMARGYTVERYRRIVNRIRELMPDAAI 330
Query: 296 TTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERS 355
+TDVIV FPG ++ + + + YSPR T A NQ+ K ER
Sbjct: 331 STDVIVAFPGETDAQFQNTLNLVEEVGFDQVNTAAYSPRPNTPAATWSNQLPEVVKVERL 390
Query: 356 KKLIELNRINEKAFAE---KYIGEVIDVLFEEEVELGSGVYTGYTR----NYIKVNAKAD 408
K +LN + E+ + +Y G+V VL E G TR +
Sbjct: 391 K---QLNALVERVALQRNSRYSGKVEQVLAEGINPKKPQQLMGRTRTNRLTFFAAEGPQS 447
Query: 409 CNVS-GKILNVKINS 422
C S G +++V+INS
Sbjct: 448 CRYSPGDLVDVQINS 462
>MIAB_METFK RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 442
Score = 204 bits (518), Expect = 2e-54
Identities = 137/439 (31%), Positives = 203/439 (46%), Gaps = 9/439 (2%)
Query: 2 KVAFATLGCRVNTYESEAMTEKF-VREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
++ T GC++N Y+S M + EG + D E ADV ++NTCSV +K +
Sbjct: 6 RIFIKTFGCQMNEYDSSRMLDMLQAAEGMQTTDNPEEADVILLNTCSVREKAQEKVFSHL 65
Query: 61 GR---ARRQNPEAIIAVVGCYA-QIAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR + +NP+ +I V GC A Q + V+ VDVV G + + + R G
Sbjct: 66 GRFAPLKEKNPDLVIGVGGCVASQEGESIVARAPYVDVVFGPQTLHRLPDMIESKRRTGI 125
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
QV + K F+ L AFL I +GC+++C +C++PYTRG S+ +
Sbjct: 126 SQVDISFPEVEK-FDHLPPPRVEGAA-AFLSIMEGCSKYCTFCVVPYTRGDEVSRPFDDI 183
Query: 177 LDEIRSLAEHGFKEIILSGIHTASYGVDL--DEKVTLVDLLEEIEKIDGIERVRIGSIDP 234
L E LAE G KEI L G + Y + E L L+E + +I IER+R + P
Sbjct: 184 LTEAIQLAEQGVKEITLLGQNVNGYRSETADGEMADLALLIEYLAEIPEIERIRYTTSHP 243
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
T ++ + KL H HL +Q+G D L M R YT +Y+ I+ LR D+
Sbjct: 244 NEMTPALIDCYARIPKLVSHLHLPVQAGSDRVLMNMKRNYTVLQYKSIIRKLRAARPDIC 303
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
I++D IVGFPG ++ + + F YSPR GT A + + +K R
Sbjct: 304 ISSDFIVGFPGETEQDFEATMKLMRDVGFDFSFSFIYSPRPGTPASYLPDDCSPEEKQSR 363
Query: 355 SKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSGK 414
+L LN KA + +G + VL E + G T N VN D + +
Sbjct: 364 LSRLQALNEAQGKAISASMVGSIQRVLIESVSAKRADELAGRTDNNRIVNFPGDTALINQ 423
Query: 415 ILNVKINSFEGEVAEGEIV 433
+NV+I GE+V
Sbjct: 424 FVNVRITEALSHTLRGELV 442
>MIAB_DESOH RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 466
Score = 204 bits (518), Expect = 2e-54
Identities = 132/412 (32%), Positives = 211/412 (51%), Gaps = 15/412 (3%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIGRA--- 63
T+GC++N Y+S ++ G+ V+ E AD+ VNTC++ +K+ +GR
Sbjct: 8 TIGCQMNVYDSSQLSAILTAMGHRSVNAPEQADLVFVNTCTIRAKAKQKATSFVGRLAAM 67
Query: 64 RRQNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGKQ--QVK 120
+R P+ I+ V GC AQ ++ VD+V G+ G + ++ +G + V+
Sbjct: 68 KRARPDMIVGVGGCLAQEEGRQLLDAFPCVDIVFGTHALGRLPGHIQAVAHQGDRIVDVE 127
Query: 121 VGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDEI 180
+ A + V L+ + + T F+ I GC+ FC YC++PY RG S+ P+ +LDEI
Sbjct: 128 MTAAIDESVHA-LQGPDSSGVT-GFITIMRGCDNFCTYCVVPYVRGRETSRAPEHILDEI 185
Query: 181 RSLAEHGFKEIILSGIHTASYGVDLDEKVTLVDLLEEIEKIDGIERVRIGSIDPTFFTED 240
R+ G +EI L G + SYG + + DLL + +IDG+ R+R + P + +
Sbjct: 186 RARVAGGLREITLLGQNVNSYG-QKEGLCSFADLLARVNEIDGLHRIRFTTSHPKDLSPE 244
Query: 241 VVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITTDVI 300
+ +L KLC H HL QSG DA LKRMNRRYT + Y + ++ LR+ ++++TD+I
Sbjct: 245 LAAAFTSLDKLCSHVHLPAQSGSDAVLKRMNRRYTRQAYLEKLHWLREAQPGMALSTDII 304
Query: 301 VGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSKKLIE 360
VGFPG ++++ F YS R A + +VD +K +R L+E
Sbjct: 305 VGFPGETEQDFLQTLDLIEKVRYDSIFAFMYSDRPLAPARAFDGKVDEAEKQQRIYALLE 364
Query: 361 L-NRIN-EKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCN 410
L NRI EK A + G V VL E + + S T + ++ + CN
Sbjct: 365 LQNRITAEKNRALE--GRVEQVLVEGKSK--SSGRNDITADTVQWTGRTTCN 412
>MIAB_COREF RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 524
Score = 204 bits (518), Expect = 2e-54
Identities = 130/438 (29%), Positives = 218/438 (49%), Gaps = 23/438 (5%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIGRAR-- 64
T GC++N ++SE ++ GY + D+ V NTC+V D++ +G R
Sbjct: 33 TFGCQMNVHDSERLSGLLEEAGYTAAGEGDTPDLIVFNTCAVRENADQRLYGTLGNLRAV 92
Query: 65 -RQNPEAIIAVVGCYAQI-APTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVKVG 122
+P IAV GC AQ T V VDVV G+ N + +N+A K +V++
Sbjct: 93 KENHPGMQIAVGGCLAQKDKDTVVKKAPWVDVVFGTHNIASLPTLLNRAEHNQKAEVEIV 152
Query: 123 AVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDEIRS 182
L+ F + + + ++ + GCN C +C++P RG + P +L E+++
Sbjct: 153 DSLEQ--FPSVLPAKRESAYAGWVSVSVGCNNTCTFCIVPSLRGKEQDRRPGDILAEVQA 210
Query: 183 LAEHGFKEIILSGIHTASYGVDL------DEKVTLVDLLEEIEKIDGIERVRIGSIDPTF 236
L + G E+ L G + +YGV+ ++ LL I+G+ERVR S P
Sbjct: 211 LVDQGVSEVTLLGQNVNAYGVNFVDPELERDRSAFSKLLRACGDIEGLERVRFTSPHPAE 270
Query: 237 FTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSIT 296
FT DV+ + +CP H+ LQSG D LK M R Y + ++ I++ +R KI SIT
Sbjct: 271 FTSDVIDAMAETPNICPQLHMPLQSGSDRVLKEMRRSYRSAKFLAILDEVRAKIPHASIT 330
Query: 297 TDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSK 356
TD+IVGFPG +++ + + F+YSPR GT A + ENQV ER +
Sbjct: 331 TDIIVGFPGETEEDFQATLDVVEKARFTSAYTFQYSPRPGTPAADYENQVPKKVVQERYE 390
Query: 357 KLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSGKIL 416
+L+ L + +K+IG+ +++L V+ G G T+ +++ +A G+++
Sbjct: 391 RLMALQERICEEENQKFIGQTVELL----VQAGGGRKNDATK---RMSGRAR---DGRLV 440
Query: 417 N-VKINSFEGEVAEGEIV 433
+ + + +GE+ G++V
Sbjct: 441 HFTPVGTIDGEIRPGDVV 458
>MIAB_RHIE6 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 469
Score = 203 bits (517), Expect = 2e-54
Identities = 140/451 (31%), Positives = 221/451 (49%), Gaps = 24/451 (5%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIG 61
KV T GC++N Y+S M++ R+GYE + E AD+ ++NTC + +K +G
Sbjct: 23 KVFIKTYGCQMNVYDSTRMSDALARDGYEPTEDMEEADLVLLNTCHIREKAAEKVYSALG 82
Query: 62 RARRQNP-------EAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARD 113
R R E +I V GC AQ E+ VDVV+G + + + +A+
Sbjct: 83 RLREMKKKKAADGREMMIGVTGCVAQAEGEEILRRAPAVDVVIGPQTYHRLPEALRRAQ- 141
Query: 114 EGKQQVKVGAVLKNKVFEDLKIEEYNNKTRA-----FLKIQDGCNRFCAYCLIPYTRGSV 168
+G++ V +++K FE L I E + K RA FL +Q+GC++FC +C++PYTRGS
Sbjct: 142 QGQRVVDTEYAIEDK-FEHLPIAE-SRKIRARGVTAFLTVQEGCDKFCTFCVVPYTRGSE 199
Query: 169 CSKDPKKVLDEIRSLAEHGFKEIILSGIHTASY--GVDLDEKVTLVDLLEEIEKIDGIER 226
S+ ++++E L E G +EI L G + ++ E +L DLL + +I G+ R
Sbjct: 200 VSRPVSQIVEEAEKLVEAGVREITLLGQNVNAWHGAGPRGEAWSLGDLLYRLAEIPGLAR 259
Query: 227 VRIGSIDPTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLL 286
+R + P + ++ L+ L P+ HL +QSG D LK MNRR+TA EY ++ +
Sbjct: 260 LRYTTSHPRDMDDRLIEAHRDLRALMPYLHLPVQSGSDRILKAMNRRHTAAEYLSLIERI 319
Query: 287 RDKIEDVSITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQV 346
R D++++ D I GFPG ++ + ++ FKYS R GT +++QV
Sbjct: 320 RTVRPDIALSGDFITGFPGETDADFEDTLRLVEEVRYAQAFSFKYSTRPGTPGAELKDQV 379
Query: 347 DGNKKDERSKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAK 406
FAE IG+ ID+L E+ + G G + VN
Sbjct: 380 PEEIXXXXXXXXXXXXXXXXXEFAESCIGKEIDLLLEKPGRM-PGQLIGRSPWLQSVNVD 438
Query: 407 ADCNVSGKILNVKI-----NSFEGEVAEGEI 432
A + G I+ V+I NS E AE +
Sbjct: 439 AKASQIGDIIKVRITGTGTNSLFAERAEAAV 469
>MIAB_ALHEH RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 448
Score = 203 bits (517), Expect = 2e-54
Identities = 139/438 (31%), Positives = 214/438 (48%), Gaps = 8/438 (1%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVRE-GYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
+V T GC++N Y+S+ M + V+E GY VD ADV ++NTCSV +K +
Sbjct: 4 RVYVKTHGCQMNEYDSDKMADVLVKERGYTRVDSPGDADVILLNTCSVREKAQEKVFSEL 63
Query: 61 GRAR--RQNPEAIIAVVGCYA-QIAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGKQ 117
GR + + A+I V GC A Q V VDVV G + + +++ARD G+
Sbjct: 64 GRWKDYKTRNGAVIGVGGCVASQEGEAIVQRAPHVDVVFGPQTLHRLPEMIDRARDGGRS 123
Query: 118 QVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVL 177
V V K F+ L T AF+ I +GC+++C++C++PYTRG S+ + V+
Sbjct: 124 VVDVSFPEIEK-FDRLPEPRAEGPT-AFVSIMEGCSKYCSFCVVPYTRGEEISRPFEDVI 181
Query: 178 DEIRSLAEHGFKEIILSGIHTASY-GVDLDEKVT-LVDLLEEIEKIDGIERVRIGSIDPT 235
E+ SLAE G +E+ L G + +Y G D V L L+ + +DGI R+R + P
Sbjct: 182 AEVASLAEQGVREVTLLGQNVNAYRGPMADGTVCDLALLIHYVAALDGIGRIRFTTSHPV 241
Query: 236 FFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSI 295
F++ ++ KL H HL +QSG D LK M R +TA EY D + ++ +SI
Sbjct: 242 EFSDSLIEAYREEPKLAGHLHLPVQSGSDLVLKLMKRGHTAAEYLDKIERIKAARPGISI 301
Query: 296 TTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERS 355
+D IVG+PG ++ + ++ F YSPR GT A ++ + K ER
Sbjct: 302 ASDFIVGYPGESEADFEDTLRLIEAVGFDQSFSFLYSPRPGTPAASLSDSTPAEVKRERL 361
Query: 356 KKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSGKI 415
+L E N + +E +G V VL + +G T N VN + G
Sbjct: 362 YRLQETINANARRISESMVGTVQRVLVDGRSRKDPNEISGRTENNRVVNFAGHPRLIGHF 421
Query: 416 LNVKINSFEGEVAEGEIV 433
+ V+I + GE++
Sbjct: 422 VEVRITEAKPNSLRGELL 439
>MIAB_TRIEI RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 451
Score = 203 bits (516), Expect = 3e-54
Identities = 131/444 (29%), Positives = 217/444 (48%), Gaps = 21/444 (4%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIGRA--- 63
T GC++N +SE M G + AD+ + NTC++ + ++K +GR
Sbjct: 12 TFGCQMNKADSERMAGILDNMGLISSEDPNKADIILYNTCTIRDNAEQKVYSYLGRQAKR 71
Query: 64 RRQNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVKVG 122
+ + P+ + V GC AQ + + +D+++G ++ + + + + G Q V
Sbjct: 72 KHKQPDLTLIVAGCVAQQEGAALLRRVPELDLIMGPQHANRLQDLLEQVFN-GNQVVATE 130
Query: 123 AVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDEIRS 182
+ + ED+ ++K A++ I GCN C YC++P RG S+ P+ + E+
Sbjct: 131 PI---HIVEDITKPRRDSKITAWVNIIYGCNEHCTYCVVPSVRGVEQSRTPEAIRAEMEE 187
Query: 183 LAEHGFKEIILSGIHTASYGVDLD-------EKVTLVDLLEEIEKIDGIERVRIGSIDPT 235
L G++EI L G + +YG DL K T DLL + + G+ER+R + P
Sbjct: 188 LGRQGYQEITLLGQNIDAYGRDLPGVTKEGRNKYTFTDLLYYVHDVPGVERIRFATSHPR 247
Query: 236 FFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSI 295
+FTE ++R L K+C HFH+ QSG + LK M R YT ++Y I++ +R+ + D SI
Sbjct: 248 YFTERLIRACAELPKVCEHFHIPFQSGDNKLLKAMARGYTHEKYRRIIDKIRELMPDASI 307
Query: 296 TTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERS 355
+ D IVGFPG ++ I + + YSPR GT A ENQ+ K +R
Sbjct: 308 SADAIVGFPGETEAQFENTLKLVEDIGFDQLNTAAYSPRPGTPAALWENQLGEEVKSDRL 367
Query: 356 KKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKAD-CNVSGK 414
++L L + +++Y+G + +VL E+ + G TR + D + GK
Sbjct: 368 QRLNHLVGVKAADRSQRYMGRIEEVLVEDMNPKNAAQVMGRTRGNRLTFFEGDIAQLKGK 427
Query: 415 ILNVKIN-----SFEGEVAEGEIV 433
++ VKI S GEV E +V
Sbjct: 428 LVKVKITEVRPFSLTGEVKEFVLV 451
>MIAB_RALSO RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 457
Score = 203 bits (516), Expect = 3e-54
Identities = 148/449 (32%), Positives = 213/449 (47%), Gaps = 24/449 (5%)
Query: 2 KVAFATLGCRVNTYESEAMTEKF-VREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
KV T GC++N Y+S+ M + EG D E ADV + NTCSV +K +
Sbjct: 3 KVFIKTFGCQMNEYDSDKMADVLNAAEGLVPTDTPEDADVILFNTCSVREKAQEKVFSDL 62
Query: 61 GRARR---QNPEAIIAVVGCYA-QIAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR + +NP+ ++ V GC A Q + V+ VDVV G + + ++ R G+
Sbjct: 63 GRVKALKARNPDLVVGVGGCVASQEGASIVARAPYVDVVFGPQTLHRLPELIDARRRTGR 122
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
QV V K F+ L + AF+ I +GC+++C+YC++PYTRG S+ + V
Sbjct: 123 PQVDVSFPEIEK-FDHLPPARVEGPS-AFVSIMEGCSKYCSYCVVPYTRGEEVSRPFEDV 180
Query: 177 LDEIRSLAEHGFKEIILSGIHTASYGVDL---DEKVTLVDLLEEIEKIDGIERVRIGSID 233
L E+ LAE G +E+ L G + +Y + E+ LLE + +I GIER+R +
Sbjct: 181 LAEVAGLAEQGVREVTLLGQNVNAYIGKMGGTSERADFALLLEYVAEIPGIERIRYTTSH 240
Query: 234 PTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDV 293
P FT ++ +KL H HL +Q G D L M R YT EY+ I+ LR D+
Sbjct: 241 PKEFTARLIEAYATNRKLVDHLHLPVQHGSDRILMAMKRGYTVLEYKSIIRKLRAIRPDI 300
Query: 294 SITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDE 353
SI TD IVGFPG + I + F YSPR GT A N+ + K E
Sbjct: 301 SIATDFIVGFPGETDADFAKTMDLVHEIGYDNSFSFIYSPRPGTPAANLHDDTPHAVKLE 360
Query: 354 RSKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVN--------A 405
R K L N +E +G V +L E G T N VN A
Sbjct: 361 RLKHLQATIDANMARISEGMVGSVQRILVEGPSRKDPSELHGRTENNRVVNFALPDLPQA 420
Query: 406 KADCNVSGKILNVKI-----NSFEGEVAE 429
+ D + G++L+V+I +S G+V E
Sbjct: 421 RRD-QLVGQMLDVRIVHAYPHSLRGDVVE 448
>MIAB_LEPBP RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 458
Score = 203 bits (516), Expect = 3e-54
Identities = 136/389 (34%), Positives = 218/389 (56%), Gaps = 14/389 (3%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKK---SRQ 58
KV T GC++N Y+S + E F +E YE + E +D+ +NTC+V K Q
Sbjct: 19 KVYVETYGCQMNEYDSGIVKELFRKEHYETTNVVEESDIIFLNTCAVRENAHAKIYGRLQ 78
Query: 59 IIGRARRQNPEAIIAVVGCYAQIAPTEVSGIE-GVDVVLGSRNKGEIVYYVNKARDEGKQ 117
+G +++NP +I V+GC AQ ++ E +D+++G N + + R+ G++
Sbjct: 79 SLGYLKKKNPNLVIGVLGCMAQNLGEDLFHQELPLDLIVGPDNYRTLPELIQNIRN-GEK 137
Query: 118 QVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVL 177
V++ + +++ +++L+ + N +AF+ I GCN FC +C++PYTRG S++PK ++
Sbjct: 138 DVQLTRLSRSETYDELEPKVVNG-IQAFVTIMRGCNNFCTFCVVPYTRGRERSREPKSII 196
Query: 178 DEIRSLAEHGFKEIILSGIHTASYGVDLDEKVTLVDLLEEIEKIDGIERVRIGSIDPTFF 237
EI+ L E G K++ L G + SY E L+E I K IERVR S P F
Sbjct: 197 HEIKQLQEMGVKQVTLLGQNVNSYSY---ESYDFCALVESILKETTIERVRFTSPHPKDF 253
Query: 238 TEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITT 297
+ ++ + + H+ LQ+G L+ M R YT +EY D+V ++ I D+ IT+
Sbjct: 254 PDHLISLMAKEDRFSSQIHMPLQAGSSKVLRDMKRSYTKEEYLDLVKKIQSVIPDIGITS 313
Query: 298 DVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSKK 357
D+IVGFPG +++FKYS R+GT A+ +D ++ +S++
Sbjct: 314 DIIVGFPGXXXXXXXXXXXXXXXXXXDMSYMFKYSEREGTIAK--RKFIDDVPEEVKSRR 371
Query: 358 LIELNRINEKAFAEK---YIGEVIDVLFE 383
LIEL + K EK IG++ +L E
Sbjct: 372 LIELVELQTKISLEKNTSKIGKIFSILIE 400
>MIAB_LEPBA RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 458
Score = 203 bits (516), Expect = 3e-54
Identities = 136/389 (34%), Positives = 218/389 (56%), Gaps = 14/389 (3%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKK---SRQ 58
KV T GC++N Y+S + E F +E YE + E +D+ +NTC+V K Q
Sbjct: 19 KVYVETYGCQMNEYDSGIVKELFRKEHYETTNVVEESDIIFLNTCAVRENAHAKIYGRLQ 78
Query: 59 IIGRARRQNPEAIIAVVGCYAQIAPTEVSGIE-GVDVVLGSRNKGEIVYYVNKARDEGKQ 117
+G +++NP +I V+GC AQ ++ E +D+++G N + + R+ G++
Sbjct: 79 SLGYLKKKNPNLVIGVLGCMAQNLGEDLFHQELPLDLIVGPDNYRTLPELIQNIRN-GEK 137
Query: 118 QVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVL 177
V++ + +++ +++L+ + N +AF+ I GCN FC +C++PYTRG S++PK ++
Sbjct: 138 DVQLTRLSRSETYDELEPKVVNG-IQAFVTIMRGCNNFCTFCVVPYTRGRERSREPKSII 196
Query: 178 DEIRSLAEHGFKEIILSGIHTASYGVDLDEKVTLVDLLEEIEKIDGIERVRIGSIDPTFF 237
EI+ L E G K++ L G + SY E L+E I K IERVR S P F
Sbjct: 197 HEIKQLQEMGVKQVTLLGQNVNSYSY---ESYDFCALVESILKETTIERVRFTSPHPKDF 253
Query: 238 TEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITT 297
+ ++ + + H+ LQ+G L+ M R YT +EY D+V ++ I D+ IT+
Sbjct: 254 PDHLISLMAKEDRFSSQIHMPLQAGSSKVLRDMKRSYTKEEYLDLVKKIQSVIPDIGITS 313
Query: 298 DVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSKK 357
D+IVGFPG +++FKYS R+GT A+ +D ++ +S++
Sbjct: 314 DIIVGFPGXXXXXXXXXXXXXXXXXXDMSYMFKYSEREGTIAK--RKFIDDVPEEVKSRR 371
Query: 358 LIELNRINEKAFAEK---YIGEVIDVLFE 383
LIEL + K EK IG++ +L E
Sbjct: 372 LIELVELQTKISLEKNTSKIGKIFSILIE 400
>MIAB_DESDG RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 464
Score = 203 bits (516), Expect = 3e-54
Identities = 125/382 (32%), Positives = 189/382 (49%), Gaps = 6/382 (1%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIGRARRQ 66
T GC++N +S+ + G+ V +E A +Y++NTCSV + ++K ++GR RR+
Sbjct: 10 TFGCQMNVNDSDWLARALEARGFTQVPEHEAA-IYIINTCSVRDKPEQKVYSLLGRIRRE 68
Query: 67 NPE---AIIAVVGCYAQ-IAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVKVG 122
+ V GC AQ I V +V G+ + + E ++ +
Sbjct: 69 TKNRRNVTVCVGGCVAQQIGKGFFKRFSQVRLVFGTDGAASAPQAIERLVQEPHARISLL 128
Query: 123 AVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDEIRS 182
+ D E A++ I GCN FCAYC++PYTRG S+ VLDE R+
Sbjct: 129 DFSEEFPERDAGWENGEVPVSAYVNIMQGCNNFCAYCIVPYTRGRQKSRSSAAVLDECRT 188
Query: 183 LAEHGFKEIILSGIHTASYGVDLD-EKVTLVDLLEEIEKIDGIERVRIGSIDPTFFTEDV 241
L +G +EI L G + SYG+D + T LL ++ I G+ER+R + P +V
Sbjct: 189 LVGNGAREITLLGQNVNSYGLDPHGDGTTFARLLHDVAAIPGLERLRFMTPHPKDIAGEV 248
Query: 242 VRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITTDVIV 301
+ ALK LCP HL LQSG D LK M R+Y Y DIV L+ D+ IT+D+IV
Sbjct: 249 IEAFGALKNLCPRVHLPLQSGSDRVLKAMGRKYDMARYMDIVTRLKAVRPDIQITSDLIV 308
Query: 302 GFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSKKLIEL 361
GFPG ++ + ++ F YS R GTRAE + ++ +K R +L E+
Sbjct: 309 GFPGETEADFEQTLEAMRTVPFVQSFSFIYSDRPGTRAEMLPGKLSREEKTARLVRLQEV 368
Query: 362 NRINEKAFAEKYIGEVIDVLFE 383
+A + +G+ + VLFE
Sbjct: 369 QNEYSEAALQAMVGKTVMVLFE 390
>MIAB_BURM1 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 457
Score = 202 bits (515), Expect = 3e-54
Identities = 141/446 (31%), Positives = 212/446 (47%), Gaps = 15/446 (3%)
Query: 2 KVAFATLGCRVNTYESEAMTEKF-VREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
KV T GC++N Y+S+ M + EG E D E AD+ + NTCSV +K +
Sbjct: 4 KVYVKTFGCQMNEYDSDKMVDVLNAAEGLEKTDSPEDADIILFNTCSVREKAQEKVFSDL 63
Query: 61 GRARR---QNPEAIIAVVGCYA-QIAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR R P+ +I V GC A Q + VS VD+V G + + ++ R G+
Sbjct: 64 GRVRELKEAKPDLLIGVGGCVASQEGASIVSRAPYVDLVFGPQTLHRLPQMIDARRTSGR 123
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
QV + K F+ L T AF+ I +GC+++C+YC++PYTRG S+ V
Sbjct: 124 AQVDITFPEIEK-FDHLPPARVEGPT-AFVSIMEGCSKYCSYCVVPYTRGDEVSRPLDDV 181
Query: 177 LDEIRSLAEHGFKEIILSGIHTASYGVDL----DEKVTLVDLLEEIEKIDGIERVRIGSI 232
L E+ LA+ G +E+ L G + +Y L E L+E + I GIER+R +
Sbjct: 182 LTEVAGLADQGVREVTLLGQNVNAYRGALTAGSSEVADFATLIEYVADIPGIERIRYTTS 241
Query: 233 DPTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIED 292
P FT+ ++ + KL H HL +Q G D L M R YT EY+ ++ LR D
Sbjct: 242 HPKEFTQRLIDTYAKVPKLVNHLHLPVQHGSDRILMAMKRGYTVLEYKSVIRKLRAIRPD 301
Query: 293 VSITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKD 352
+S++TD+IVGFPG + + + F YSPR GT A N+ + + K
Sbjct: 302 LSLSTDLIVGFPGETEEDFDKMMALVHDMRYDTSFSFIYSPRPGTPAANLHDDTPRDVKL 361
Query: 353 ERSKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADC--- 409
+R + + N ++ +G+V +L E G T N VN A
Sbjct: 362 KRLQLVQATIEENVARISQSMVGKVERILVEGPSRKDPNELAGRTENNRVVNFPAPLAAH 421
Query: 410 -NVSGKILNVKINSFEGEVAEGEIVL 434
+ G++++VKIN GE+VL
Sbjct: 422 PRLIGQMIDVKINHAYPHSLRGELVL 447
>MIAB_CYAA5 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 448
Score = 202 bits (514), Expect = 4e-54
Identities = 127/439 (28%), Positives = 218/439 (49%), Gaps = 16/439 (3%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIGRA--- 63
T GC++N +SE M G++ + AD+ + NTC++ + ++K +GR
Sbjct: 14 TFGCQMNKADSERMAGILEDMGFKWSEDPNTADLILYNTCTIRDNAEQKVYSYLGRQAKR 73
Query: 64 RRQNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVKVG 122
+ ++P+ + V GC AQ ++ + +D+V+G ++ + + + D G Q V
Sbjct: 74 KHEHPDLTLVVAGCVAQQEGEKILRRVPELDLVMGPQHANRLQDLLTQVFD-GNQVVATE 132
Query: 123 AVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDEIRS 182
+ + ED+ ++ A++ I GCN C+YC++P RG S+ P+ + E+
Sbjct: 133 PI---HIVEDITKPRRDSTVTAWVNIIYGCNERCSYCVVPNVRGVEQSRTPEAIYAEMEL 189
Query: 183 LAEHGFKEIILSGIHTASYGVDLD-------EKVTLVDLLEEIEKIDGIERVRIGSIDPT 235
L + G+KE+ L G + +YG DL + TL DLL ++ I GIER+R + P
Sbjct: 190 LGKQGYKEVTLLGQNIDAYGRDLPGVTETGRHQHTLTDLLYQVHDISGIERLRFATSHPR 249
Query: 236 FFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSI 295
+FTE +++ L K+C HFH+ QSG + LK M R YT ++Y I++ +R+ + D SI
Sbjct: 250 YFTERLIKACHELPKVCEHFHIPFQSGDNDVLKAMKRGYTHEKYRHIIDKIREYMPDASI 309
Query: 296 TTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERS 355
+ D IVGFPG + I + + YSPR GT A +NQ+ K +R
Sbjct: 310 SADAIVGFPGETEAQFENTLKLVDDIGFDQLNTAAYSPRPGTPAAIWDNQLSEEIKSDRL 369
Query: 356 KKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCN-VSGK 414
++L L +++Y+ + +VL E+E + G TR + D + G+
Sbjct: 370 QRLNHLVAQKAAQRSQRYLNRIEEVLVEDENPKDNTQVMGRTRGNRLTFFEGDIEALKGQ 429
Query: 415 ILNVKINSFEGEVAEGEIV 433
++ VKI GE +
Sbjct: 430 LIKVKITEVRAFSLTGEAI 448
>MIAB_CLAM3 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 528
Score = 202 bits (514), Expect = 4e-54
Identities = 134/431 (31%), Positives = 211/431 (48%), Gaps = 17/431 (3%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIGR---A 63
T GC++N ++SE +T GY + E AD+ V+NTC+V D K +G
Sbjct: 25 TYGCQMNVHDSERLTGSLEAAGYVSAEGAE-ADIVVINTCAVRENADNKLYGNLGHLAGV 83
Query: 64 RRQNPEAIIAVVGCYAQI-APTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVKVG 122
+R++ IAV GC AQ T + VDVV G+ N G + + +AR G+ Q+++
Sbjct: 84 KRRHEGMQIAVGGCLAQKDRATVLEKAPWVDVVFGTHNMGALPTLLERARHNGEAQLEIL 143
Query: 123 AVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDEIRS 182
L+ F + + ++ I GCN C +C++P RG + P +L EI++
Sbjct: 144 ESLET--FPSTLPTKRDEIASGWVSISVGCNNTCTFCIVPALRGKEKDRRPGDILAEIQA 201
Query: 183 LAEHGFKEIILSGIHTASYGVDLDEKVTLVDLLEEIEKIDGIERVRIGSIDPTFFTEDVV 242
L + G E+ L G + SYGV+ ++ LL I+G+ER+R S P FT+DV+
Sbjct: 202 LVDDGAVEVTLLGQNVNSYGVEFGDRQAFGKLLRAAGAIEGLERIRFTSPHPAAFTDDVI 261
Query: 243 RRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITTDVIVG 302
+ + P H+ LQSG D LK M R Y ++ + I++ +R +I D +ITTD+IVG
Sbjct: 262 DAMAETPAVMPQLHMPLQSGSDRVLKAMRRSYRSERFLGILDRVRTRIPDAAITTDIIVG 321
Query: 303 FPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSKKLIELN 362
FPG ++ S F+YS R GT A ME QV + ER +L L
Sbjct: 322 FPGETEEDFQETLRVVEAARFSSAFTFQYSIRPGTPAATMEEQVPADVVKERYGRLTALQ 381
Query: 363 RINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSGKILNVKINS 422
++ +G ++VL V G G TR +V +A G+++++ + +
Sbjct: 382 ERISHEENQRVVGRTVEVL----VSAHEGRKDGDTR---RVTGRAQ---DGRLVHLDVPA 431
Query: 423 FEGEVAEGEIV 433
GE G+ V
Sbjct: 432 GSGEPRPGDAV 442
>MIAB_BURTA RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 457
Score = 202 bits (514), Expect = 4e-54
Identities = 141/446 (31%), Positives = 213/446 (47%), Gaps = 15/446 (3%)
Query: 2 KVAFATLGCRVNTYESEAMTEKF-VREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
KV T GC++N Y+S+ M + EG E D E AD+ + NTCSV +K +
Sbjct: 4 KVYVKTFGCQMNEYDSDKMVDVLNAAEGLEKTDSPEDADIILFNTCSVREKAQEKVFSDL 63
Query: 61 GRARR---QNPEAIIAVVGCYA-QIAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR R P+ +I V GC A Q + V+ VD+V G + + ++ R+ G+
Sbjct: 64 GRVRELKEAKPDLLIGVGGCVASQEGASIVARAPYVDLVFGPQTLHRLPQMIDARRESGR 123
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
QV + K F+ L + AF+ I +GC+++C+YC++PYTRG S+ V
Sbjct: 124 AQVDITFPEIEK-FDHLPPARVEGPS-AFVSIMEGCSKYCSYCVVPYTRGDEVSRPLDDV 181
Query: 177 LDEIRSLAEHGFKEIILSGIHTASYGVDLD----EKVTLVDLLEEIEKIDGIERVRIGSI 232
L EI LA+ G +E+ L G + +Y L E L+E + I GIER+R +
Sbjct: 182 LTEIAGLADQGVREVTLLGQNVNAYRGALTAGAHEIADFATLIEYVADIPGIERIRYTTS 241
Query: 233 DPTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIED 292
P FT+ ++ + KL H HL +Q G D L M R YT EY+ ++ LR +
Sbjct: 242 HPKEFTQRLLDVYAKVPKLVDHLHLPVQHGSDRILMAMKRGYTVLEYKSLIRKLRAIRPN 301
Query: 293 VSITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKD 352
+S++TD+IVGFPG + ++ + F YSPR GT A N+ + K
Sbjct: 302 LSLSTDIIVGFPGETEADFDKTMALVHEMSYDTSFSFIYSPRPGTPAANLADDTPREVKL 361
Query: 353 ERSKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVN----AKAD 408
ER + L N ++ +G+V +L E G T N VN A
Sbjct: 362 ERLQHLQATIEENVARISQSMLGKVERILVEGPSRKDPNELAGRTENNRVVNFPAPLTAH 421
Query: 409 CNVSGKILNVKINSFEGEVAEGEIVL 434
+ G++++VKIN GE+VL
Sbjct: 422 ARLIGQMIDVKINHAYPHSLRGELVL 447
>MIAB_SILPO RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 439
Score = 202 bits (513), Expect = 6e-54
Identities = 137/438 (31%), Positives = 223/438 (50%), Gaps = 15/438 (3%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIG 61
K+ T GC++N Y+SE M E +GY E AD+ ++NTC + +K +G
Sbjct: 6 KLFIKTYGCQMNVYDSERMAEALGGQGYVETQSAEDADMILLNTCHIREKAAEKVYSELG 65
Query: 62 R---ARRQNPEAIIAVVGCYAQIAPTEVSGIEG-VDVVLGSRNKGEIVYYVNKARDEGKQ 117
R + P+ I V GC AQ E+ + VD+V+G ++ + K R G++
Sbjct: 66 RFKGLKAAKPDLKIGVAGCVAQAEGEEIMRRQPLVDLVVGPQSYHRLPELEAKTR-AGEK 124
Query: 118 QVKVGAVLKNKVFEDLKIEEYNNK-TRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
+ ++K FE LK + AFL +Q+GC++FCA+C++PYTRG+ S+ ++
Sbjct: 125 ALDTDFPEEDK-FEKLKGRPKAKRGPTAFLTVQEGCDKFCAFCVVPYTRGAEVSRPADRI 183
Query: 177 LDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKVTLVDLLEEIEKIDGIERVRIGSIDPT 235
L E L E G +EI L G + +Y G + +TL L+ E++KIDG+ER+R + P
Sbjct: 184 LREANELVERGVREITLLGQNVNAYHGAGPNGDMTLAGLIWELDKIDGLERIRFTTSHPN 243
Query: 236 FFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSI 295
+D++ KL P+ HL +Q+G D LKRMNR +TA+ Y ++ +R D+ +
Sbjct: 244 DMADDLIEAHGTCAKLMPYLHLPVQAGSDKILKRMNRSHTAESYLRLIERIRAARPDIVM 303
Query: 296 TTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERS 355
+ D IVGFP ++++ + FKYS R GT A QVD + D+R
Sbjct: 304 SGDFIVGFPEETEADFQDTLDLVEQVRYGYAYSFKYSTRPGTPAAE-RAQVDPAEADDRL 362
Query: 356 KKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSGKI 415
++L + +++ + +G + VLFE+ G G + V+ + +G+I
Sbjct: 363 QRLQAVITRHQREIQDGMVGREVSVLFEKPGR-QPGQMVGKSEYLHAVHVQDPGLEAGQI 421
Query: 416 LNVKI-----NSFEGEVA 428
V+I NS GE+A
Sbjct: 422 ARVRIVASGANSLAGELA 439
>MIAB_BURA4 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 457
Score = 202 bits (513), Expect = 6e-54
Identities = 140/446 (31%), Positives = 212/446 (47%), Gaps = 15/446 (3%)
Query: 2 KVAFATLGCRVNTYESEAMTEKF-VREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
KV T GC++N Y+S+ M + EG E D E AD+ + NTCSV +K +
Sbjct: 4 KVYIKTFGCQMNEYDSDKMVDVLNAAEGLEKTDTPEDADIILFNTCSVREKAQEKVFSDL 63
Query: 61 GRARR---QNPEAIIAVVGCYA-QIAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR R P +I V GC A Q + VS VD+V G + + +++ R G+
Sbjct: 64 GRVRELKEAKPGLLIGVGGCVASQEGASIVSRAPYVDLVFGPQTLHRLPQMIDQRRASGR 123
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
QV + K F+ L + AF+ I +GC+++C+YC++PYTRG S+ V
Sbjct: 124 AQVDISFPEIEK-FDHLPPARVEGPS-AFVSIMEGCSKYCSYCVVPYTRGDEVSRPLDDV 181
Query: 177 LDEIRSLAEHGFKEIILSGIHTASYGVDLD----EKVTLVDLLEEIEKIDGIERVRIGSI 232
L E+ LA+ G +E+ L G + +Y L E L+E + I GIER+R +
Sbjct: 182 LTEVAGLADQGVREVTLLGQNVNAYRGALTAGSTEIADFATLIEYVADIPGIERIRYTTS 241
Query: 233 DPTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIED 292
P FT+ ++ + KL H HL +Q G D L M R YT EY+ ++ LR D
Sbjct: 242 HPKEFTQRLIDTYAKVPKLVSHLHLPVQHGSDRILMAMKRGYTVLEYKSVIRKLRAIRPD 301
Query: 293 VSITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKD 352
+S++TD+IVGFPG + ++ + F YSPR GT A N+ + K
Sbjct: 302 LSLSTDMIVGFPGETEEDFDKMMALVHEMSYDTSFSFIYSPRPGTPAANLHDDTPREVKL 361
Query: 353 ERSKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADC--- 409
+R + L N + +G+V +L E +G T N VN A
Sbjct: 362 KRLQHLQATIEENVARISRSMVGKVERILVEGPSRKDPNELSGRTENNRVVNFPAPLASH 421
Query: 410 -NVSGKILNVKINSFEGEVAEGEIVL 434
+ G++++VKIN GE++L
Sbjct: 422 PRLIGQMIDVKINHAYPHSLRGELLL 447
>MIAB_BARQU RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 458
Score = 202 bits (513), Expect = 6e-54
Identities = 136/447 (30%), Positives = 220/447 (49%), Gaps = 26/447 (5%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIG 61
KV T GC++N Y+S+ MT+ +GY AD+ ++NTC + +K +G
Sbjct: 16 KVFIKTYGCQMNVYDSQRMTDSLSSKGYVATQTPNDADLILLNTCHIREKAAEKLYSDLG 75
Query: 62 R------ARRQNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDE 114
R AR + +I V GC AQ E+ VD+V+G + + + + + +
Sbjct: 76 RLRVMRQARTLDKPLMIGVTGCVAQAEGNEILRRAPMVDLVIGPQMYHRLPDLLEQTK-Q 134
Query: 115 GKQQVKVGAVLKNKVFEDLKIEEYNNKT------RAFLKIQDGCNRFCAYCLIPYTRGSV 168
GK+ V+ +++K + +N + AFL +Q+GC++FC +C++PYTRG+
Sbjct: 135 GKKIVETDYPVEDKFAH---LPPHNKRAVRKRGVSAFLTVQEGCDKFCTFCVVPYTRGAE 191
Query: 169 CSKDPKKVLDEIRSLAEHGFKEIILSGIHTASYGVDLDEKVT--LVDLLEEIEKIDGIER 226
S+ +++ +E R L E G KEI L G + + + T L DLL + K+DG++R
Sbjct: 192 ISRSVEQITEEARQLIEAGVKEITLLGQNVNGWHGQIANGKTWRLGDLLYHLAKLDGLKR 251
Query: 227 VRIGSIDPTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLL 286
+R + P + ++ L L P+ HL +QSG D LK MNR++ + Y ++ +
Sbjct: 252 LRYTTSHPRDMDDSLIAAHRDLDILMPYLHLPVQSGSDRILKAMNRQHKSIHYLHLIEKI 311
Query: 287 RDKIEDVSITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQV 346
R D++ + D IVGFPG +K++ S + FKYSPR GT M+N V
Sbjct: 312 RTARPDIAFSGDFIVGFPGETDEDFEETVKLIKQVQYSSAYSFKYSPRPGTVGATMKNHV 371
Query: 347 DGNKKDERSKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAK 406
D KD R AF IG+ DVL E+V SG G + + V
Sbjct: 372 DEAVKDARXXXXXXXXXXXXNAFLRSKIGQTTDVLI-EKVGRHSGQMVGRSPWLLPVVVD 430
Query: 407 ADCNVSGKILNVKI-----NSFEGEVA 428
+ + +G ++++ I NSF GE+A
Sbjct: 431 TEAS-TGTVMSIHIKNAGSNSFVGEIA 456
>MIAB_ACTP7 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 475
Score = 202 bits (513), Expect = 6e-54
Identities = 143/444 (32%), Positives = 219/444 (49%), Gaps = 18/444 (4%)
Query: 2 KVAFATLGCRVNTYESEAMTEKF-VREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S M + G E+ D E ADV ++NTCS+ +K +
Sbjct: 3 KLHITTWGCQMNEYDSSKMADLLNSTHGLELTDKPEEADVLLLNTCSIREKAQEKVFSQL 62
Query: 61 GRAR---RQNPEAIIAVVGCYAQIAPTEVSG-IEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR + + P+ II V GC A + VD+V G + + +NK R +
Sbjct: 63 GRWKNWKKDKPDLIIGVGGCVASQEGEHIRDRAPFVDIVFGPQTLHRLPEMINKIRGGDR 122
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
V + K F+ L T AF+ I +GCN++C++C++PYTRG S+ V
Sbjct: 123 AIVDISFPEIEK-FDRLPEPRAEGPT-AFVSIMEGCNKYCSFCVVPYTRGEEVSRPVDDV 180
Query: 177 LDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKV-TLVDLLEEIEKIDGIERVRIGSIDP 234
L EI LAE G +E+ L G + +Y G D + T +LL + IDGI+RVR + P
Sbjct: 181 LFEIAQLAEQGVREVNLLGQNVNAYRGETFDGGICTFAELLRLVAAIDGIDRVRYTTSHP 240
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
FT+D++ +L HL +QSG D L M R +TA EY+ I+ LR+ ++
Sbjct: 241 IEFTDDIIEVYRDTPELVSFLHLPIQSGADRVLTMMKRNHTALEYKAIIRKLREVRPNIQ 300
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
I++D IVGFPG ++++ + F YS R GT A ++ + + +K ER
Sbjct: 301 ISSDFIVGFPGETAEDFEQTMKVIEQVNFDMSFSFIYSARPGTPAADLPDDISEEEKKER 360
Query: 355 SKKLIELNRINEKA--FAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVS 412
+L + RIN +A F+ +G VL E + TG T N VN + ++
Sbjct: 361 LARLQQ--RINHQAMQFSRAMLGTEQRVLVEGPSKKDIMELTGRTENNRIVNFQGTPDMI 418
Query: 413 GKILNVKI-----NSFEGEVAEGE 431
GK +++KI NS G+V E
Sbjct: 419 GKFVDIKITDVYTNSLRGDVVRTE 442
>MIAB_ACTP2 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 475
Score = 202 bits (513), Expect = 6e-54
Identities = 143/444 (32%), Positives = 219/444 (49%), Gaps = 18/444 (4%)
Query: 2 KVAFATLGCRVNTYESEAMTEKF-VREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S M + G E+ D E ADV ++NTCS+ +K +
Sbjct: 3 KLHITTWGCQMNEYDSSKMADLLNSTHGLELTDKPEEADVLLLNTCSIREKAQEKVFSQL 62
Query: 61 GRAR---RQNPEAIIAVVGCYAQIAPTEVSG-IEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR + + P+ II V GC A + VD+V G + + +NK R +
Sbjct: 63 GRWKNWKKDKPDLIIGVGGCVASQEGEHIRDRAPFVDIVFGPQTLHRLPEMINKIRGGDR 122
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
V + K F+ L T AF+ I +GCN++C++C++PYTRG S+ V
Sbjct: 123 AIVDISFPEIEK-FDRLPEPRAEGPT-AFVSIMEGCNKYCSFCVVPYTRGEEVSRPVDDV 180
Query: 177 LDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKV-TLVDLLEEIEKIDGIERVRIGSIDP 234
L EI LAE G +E+ L G + +Y G D + T +LL + IDGI+RVR + P
Sbjct: 181 LFEIAQLAEQGVREVNLLGQNVNAYRGETFDGGICTFAELLRLVAAIDGIDRVRYTTSHP 240
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
FT+D++ +L HL +QSG D L M R +TA EY+ I+ LR+ ++
Sbjct: 241 IEFTDDIIEVYRDTPELVSFLHLPIQSGADRVLTMMKRNHTALEYKAIIRKLREVRPNIQ 300
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
I++D IVGFPG ++++ + F YS R GT A ++ + + +K ER
Sbjct: 301 ISSDFIVGFPGETAEDFEQTMKVIEQVNFDMSFSFIYSARPGTPAADLPDDISEEEKKER 360
Query: 355 SKKLIELNRINEKA--FAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVS 412
+L + RIN +A F+ +G VL E + TG T N VN + ++
Sbjct: 361 LARLQQ--RINHQAMQFSRAMLGTEQRVLVEGPSKKDIMELTGRTENNRIVNFQGTPDMI 418
Query: 413 GKILNVKI-----NSFEGEVAEGE 431
GK +++KI NS G+V E
Sbjct: 419 GKFVDIKITDVYTNSLRGDVVRTE 442
>MIAB_SYNR3 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 453
Score = 201 bits (512), Expect = 8e-54
Identities = 126/388 (32%), Positives = 195/388 (50%), Gaps = 15/388 (3%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIGRA--- 63
T GC++N +SE M GY + AD+ + NTC++ + ++K +GR
Sbjct: 13 TFGCQMNKADSERMAGILESMGYCAGSGEDQADLVLYNTCTIRDNAEQKVYSYLGRQARR 72
Query: 64 RRQNPEAIIAVVGCYAQI-APTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVKVG 122
+R NP + V GC AQ + + + +D+V+G ++ + +++ E QQV
Sbjct: 73 KRDNPALTLVVAGCVAQQEGESLLRRVPELDLVMGPQHANRLDTLLSQV--EAGQQVV-- 128
Query: 123 AVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDEIRS 182
A + + ED+ ++ A++ + GCN C YC++P RG S+ P+ + E+
Sbjct: 129 ATDDHHILEDITTARRDSSLCAWVNVIYGCNERCTYCVVPSVRGQEQSRLPQAIRLEMEG 188
Query: 183 LAEHGFKEIILSGIHTASYGVDLD-------EKVTLVDLLEEIEKIDGIERVRIGSIDPT 235
LA G+KEI L G + +YG DL + TL DLL + + GIER+R + P
Sbjct: 189 LAASGYKEITLLGQNIDAYGRDLPGITPEGRRQNTLTDLLHHVHDVKGIERIRFATSHPR 248
Query: 236 FFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSI 295
+FTE ++ L K+C HFH+ QSG D LK M R YT Y IV +R + D +I
Sbjct: 249 YFTERLIEACAELPKVCEHFHVPFQSGDDELLKAMARGYTTARYRRIVEQIRKLMPDAAI 308
Query: 296 TTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERS 355
+ D IVGFPG + I + YSPR T A + +QV+ + K ER
Sbjct: 309 SADAIVGFPGETDAQFRRTLELVDEIGFDLLNTAAYSPRPNTPAADWPDQVEEHVKVERL 368
Query: 356 KKLIELNRINEKAFAEKYIGEVIDVLFE 383
K+L L KA +++Y+G V +VL E
Sbjct: 369 KELNALVERKAKACSQRYLGRVEEVLAE 396
>MIAB_RHOS1 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 436
Score = 201 bits (512), Expect = 8e-54
Identities = 134/434 (30%), Positives = 218/434 (50%), Gaps = 16/434 (3%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIG 61
K+ T GC++N Y+SE M E +GY + + E AD+ ++NTC + +K +G
Sbjct: 6 KLFIKTYGCQMNVYDSERMAEALGAKGYVLTEVAEEADMVLLNTCHIREKAAEKVYSDLG 65
Query: 62 RAR---RQNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGKQ 117
R R P+ I V GC AQ E+ + VD+V+G ++ + ++ R EG
Sbjct: 66 RLRPLKTAKPDLKIGVAGCVAQAEGEEILKRMPLVDLVVGPQSYHRLPDMLD--RTEGGA 123
Query: 118 QVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVL 177
+V + F+ L + AFL +Q+GC++FCA+C++PYTRG+ S+ +++
Sbjct: 124 RVVDTDFPEEDKFDHLPERKATRGPAAFLTVQEGCDKFCAFCVVPYTRGAEVSRPFARLM 183
Query: 178 DEIRSLAEHGFKEIILSGIHTASYGVDLDEKVTLVDLLEEIEKIDGIERVRIGSIDPTFF 237
E R L E G +EI L G + ++ D L L+ E+ +IDG+ER+R + P
Sbjct: 184 AEARGLVERGVREITLLGQNVNAWSSD---GRGLGGLIRELARIDGLERLRYTTSHPNDM 240
Query: 238 TEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITT 297
+D++ A KL P+ HL +QSG D LK MNR++TA+ Y ++ +R D+ +T+
Sbjct: 241 ADDLIEAHGAEPKLMPYLHLPVQSGSDRILKAMNRKHTAEHYLRLIERIRAARPDILLTS 300
Query: 298 DVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSKK 357
D IVGFPG ++ + FKYS R GT A + ++ G D R ++
Sbjct: 301 DFIVGFPGETEADFEATLDLIRAVGFGSAFSFKYSARPGTPAAE-KPELPGEVCDARLQR 359
Query: 358 LIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSGKILN 417
L L ++A +G + VL+E+ L G G + + V+ + G ++
Sbjct: 360 LQALVTEQQRAAQMAMVGREVGVLYEKAGRL-PGQMVGKSDHLHAVHVEDKAGRVGDLVR 418
Query: 418 VKI-----NSFEGE 426
V+I NS GE
Sbjct: 419 VRITASAPNSLAGE 432
>MIAB_BURCM RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 457
Score = 201 bits (512), Expect = 8e-54
Identities = 140/446 (31%), Positives = 212/446 (47%), Gaps = 15/446 (3%)
Query: 2 KVAFATLGCRVNTYESEAMTEKF-VREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
KV T GC++N Y+S+ M + EG E D E AD+ + NTCSV +K +
Sbjct: 4 KVYVKTFGCQMNEYDSDKMVDVLNAAEGLEKTDTPEDADIILFNTCSVREKAQEKVFSDL 63
Query: 61 GRARR---QNPEAIIAVVGCYA-QIAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR R P +I V GC A Q + VS VD+V G + + +++ R G+
Sbjct: 64 GRVRELKEAKPGLLIGVGGCVASQEGASIVSRAPYVDLVFGPQTLHRLPQMIDQRRASGR 123
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
QV + K F+ L + AF+ I +GC+++C+YC++PYTRG S+ V
Sbjct: 124 AQVDISFPEIEK-FDHLPPARVEGPS-AFVSIMEGCSKYCSYCVVPYTRGDEVSRPLDDV 181
Query: 177 LDEIRSLAEHGFKEIILSGIHTASYGVDLD----EKVTLVDLLEEIEKIDGIERVRIGSI 232
L E+ LA+ G +E+ L G + +Y L E L+E + I GIER+R +
Sbjct: 182 LTEVAGLADQGVREVTLLGQNVNAYRGALTAGSTEIADFATLIEYVADIPGIERIRYTTS 241
Query: 233 DPTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIED 292
P FT+ ++ + KL H HL +Q G D L M R YT EY+ ++ LR D
Sbjct: 242 HPKEFTQRLIDTYAKVPKLVSHLHLPVQHGSDRILMAMKRGYTVLEYKSVIRKLRAIRPD 301
Query: 293 VSITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKD 352
+S++TD+IVGFPG + ++ + F YSPR GT A N+ + K
Sbjct: 302 LSLSTDMIVGFPGETEEDFDKMMALVHEMSYDTSFSFIYSPRPGTPAANLHDDTPREVKL 361
Query: 353 ERSKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADC--- 409
+R + L N + +G+V +L E +G T N VN A
Sbjct: 362 KRLQHLQATIEENVARISRSMVGKVERILVEGPSRKDPNELSGRTENNRVVNFPAPLASH 421
Query: 410 -NVSGKILNVKINSFEGEVAEGEIVL 434
+ G++++VKIN GE++L
Sbjct: 422 PRLIGQMIDVKINHAYPHSLRGELLL 447
>MIAB_BRAJA RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 465
Score = 201 bits (512), Expect = 8e-54
Identities = 133/444 (29%), Positives = 220/444 (49%), Gaps = 13/444 (2%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIG 61
K+ + GC++N Y+++ M + EG+ E AD+ ++NTC + +K +G
Sbjct: 6 KLHIKSYGCQMNVYDAQRMVDTLAPEGFVETASAEDADLVILNTCHIREKASEKVYSELG 65
Query: 62 RARRQNPEAI-------IAVVGCYAQIAPTE-VSGIEGVDVVLGSRNKGEIVYYVNKARD 113
R R EA IAV GC AQ E V G ++ + + +A +
Sbjct: 66 RLRVAKDEAARGGRAMQIAVAGCVAQAEGEEIVRRAPXXXXXXGPQSYHHLPELLKRAGN 125
Query: 114 EGKQ-QVKVGAVLKNKVFEDLKIEEYNNK-TRAFLKIQDGCNRFCAYCLIPYTRGSVCSK 171
EG+ + + A K K + + AF+ +Q+GC++FC +C++PYTRG+ S+
Sbjct: 126 EGRAVETEFPAADKFGFLAQPKPDAIRARGISAFVTVQEGCDKFCTFCVVPYTRGAEVSR 185
Query: 172 DPKKVLDEIRSLAEHGFKEIILSGIHTASYGVDLDEKVT--LVDLLEEIEKIDGIERVRI 229
+++D+++ LA++G +E+ L G + +Y D + + L LLE + I GI R+R
Sbjct: 186 PVARIVDDVKRLADNGVRELTLIGQNVNAYHGDGPDGKSWPLGRLLEHLAGIPGIARLRY 245
Query: 230 GSIDPTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDK 289
+ P + ++ L L P HL +QSG D L MNR++TA +Y +++ R
Sbjct: 246 STSHPRDVDDSLIAAHRDLAALMPFVHLPVQSGSDRILAAMNRKHTADDYRRVIDRFRAA 305
Query: 290 IEDVSITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGN 349
+D++ ++D IVGFPG + +I + + FKYS R GT A +M+ V
Sbjct: 306 RQDIAFSSDFIVGFPGESEQDFLATLALVTQIGYAAAYSFKYSARPGTPAADMQETVSPA 365
Query: 350 KKDERSKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADC 409
+ D+R ++L EL + AF + IG +DVLFE G G T + A
Sbjct: 366 EMDQRLERLQELIDSQQSAFNKAAIGSTVDVLFERPAR-KDGQIVGRTAFLQPAHVMASP 424
Query: 410 NVSGKILNVKINSFEGEVAEGEIV 433
+ G+IL V+I+S E GE+V
Sbjct: 425 GIIGQILPVRIDSLERYSFLGELV 448
>MIAB_BART1 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 458
Score = 201 bits (512), Expect = 8e-54
Identities = 136/445 (30%), Positives = 215/445 (48%), Gaps = 24/445 (5%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIG 61
KV T GC++N Y+S+ MT+ +GY AD+ +VNTC + +K +G
Sbjct: 16 KVFIKTYGCQMNVYDSQRMTDSLSSQGYVTTQTPNDADLILVNTCHIREKAAEKLYSDLG 75
Query: 62 RARRQNPEAI------IAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDE 114
R R E + V GC AQ +E+ VD+V+G + + + KA+ +
Sbjct: 76 RLRVMRQERTPDKPLTVGVTGCVAQAEGSEILRRAPIVDLVIGPQMYHRLPELLEKAK-Q 134
Query: 115 GKQQVKVGAVLKNKVFEDLKIEEYNNKT------RAFLKIQDGCNRFCAYCLIPYTRGSV 168
GK+ ++ +++K + +N + AFL +Q+GC++FC +C++PYTRG+
Sbjct: 135 GKKIIETDYAVEDKFAH---LPPHNKRAVRKRGVSAFLTVQEGCDKFCTFCVVPYTRGAE 191
Query: 169 CSKDPKKVLDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKV-TLVDLLEEIEKIDGIER 226
S+ +++ +E R L E G KEI L G + + G D K L DLL + K+DG++R
Sbjct: 192 ISRSVEQITEEARQLIEAGVKEITLLGQNVNGWHGQSADGKTWRLGDLLYHLAKLDGLKR 251
Query: 227 VRIGSIDPTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLL 286
+R + P + ++ L L P+ HL +QSG D LK MNR++ + Y ++ +
Sbjct: 252 LRYTTSHPRDMDDSLIAAHRDLDMLMPYLHLPVQSGSDRILKAMNRQHKSSYYLHLIEKI 311
Query: 287 RDKIEDVSITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQV 346
R D++ + D IVGFPG ++++ S + FKYSPR GT M+N V
Sbjct: 312 RAARPDIAFSGDFIVGFPGETDEDFEETIKLIQQVQYSSAYSFKYSPRPGTVGATMKNHV 371
Query: 347 DGNKKDERSKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAK 406
D + KD R F IG+ DVL E+ SG G + + V
Sbjct: 372 DESVKDARXXXXXXXXXXXXNTFLRSKIGQKTDVLIEKPGR-HSGQMVGRSPWLLPVVVD 430
Query: 407 ADCN----VSGKILNVKINSFEGEV 427
+ + V I N NSF GE+
Sbjct: 431 TESSTGSVVEIHIKNASSNSFVGEM 455
>MIAB_RHILW RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 469
Score = 201 bits (510), Expect = 1e-53
Identities = 140/451 (31%), Positives = 221/451 (49%), Gaps = 24/451 (5%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIG 61
KV T GC++N Y+S M++ R+GYE + E AD+ ++NTC + +K +G
Sbjct: 23 KVFIKTYGCQMNVYDSTRMSDALARDGYEPTEDMEEADLVLLNTCHIREKAAEKVYSALG 82
Query: 62 RARRQNP-------EAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARD 113
R R E +I V GC AQ E+ VDVV+G + + + +A+
Sbjct: 83 RLRDMKKKKAADGREMMIGVAGCVAQAEGEEILRRAPAVDVVIGPQTYHRLPEALRRAK- 141
Query: 114 EGKQQVKVGAVLKNKVFEDLKIEEYNNKTRA-----FLKIQDGCNRFCAYCLIPYTRGSV 168
EG++ V +++K FE L I E + K RA FL +Q+GC++FC +C++PYTRGS
Sbjct: 142 EGQRVVDTDYAIEDK-FEHLPIAE-SRKIRARGVTAFLTVQEGCDKFCTFCVVPYTRGSE 199
Query: 169 CSKDPKKVLDEIRSLAEHGFKEIILSGIHTASY--GVDLDEKVTLVDLLEEIEKIDGIER 226
S+ ++++E L E G +EI L G + ++ E +L DLL + +I G+ R
Sbjct: 200 VSRPVAQIVEEAEKLVEGGVREITLLGQNVNAWHGAGPRGEAWSLGDLLYRLAEIPGLAR 259
Query: 227 VRIGSIDPTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLL 286
+R + P + ++ L+ L P+ HL +QSG D LK MNRR+TA EY ++ +
Sbjct: 260 LRYTTSHPRDMDDRLIDAHRDLRALMPYLHLPVQSGSDRILKAMNRRHTAAEYLSLIARI 319
Query: 287 RDKIEDVSITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQV 346
R D++++ D I GFPG ++ + ++ FKYS R GT +++QV
Sbjct: 320 RTVRPDIALSGDFITGFPGETDADFEDTLRLVEEVRYAQAFSFKYSTRPGTPGAELKDQV 379
Query: 347 DGNKKDERSKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAK 406
FAE IG+ ID+L E+ + + G + VN
Sbjct: 380 PEQIXXXXXXXXXXXXXXXXXEFAESCIGKEIDLLLEKPGRMPEQL-IGRSPWLQSVNVD 438
Query: 407 ADCNVSGKILNVKI-----NSFEGEVAEGEI 432
A + G I+ V+I NS E AE +
Sbjct: 439 AKASQIGDIIKVRITGTGNNSLFAERAEAAV 469
>MIAB_HAES2 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 474
Score = 201 bits (510), Expect = 1e-53
Identities = 144/444 (32%), Positives = 219/444 (49%), Gaps = 18/444 (4%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVR-EGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S M + + G E+ D E ADV ++NTCS+ +K +
Sbjct: 4 KLHIKTWGCQMNEYDSSKMADLLLNTHGLELTDVPEEADVLLLNTCSIREKAQEKVFHQL 63
Query: 61 GR---ARRQNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR ++QNP +I V GC A + S VD+V G + + +N+ R GK
Sbjct: 64 GRWKELKKQNPSLVIGVGGCVASQEGEHIRSRAPYVDIVFGPQTLHRLPEMINQIRG-GK 122
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
V + + + F+ L T AF+ I +GCN++C++C++PYTRG S+ V
Sbjct: 123 SAVVDVSFPEIEKFDRLPEPRAEGPT-AFVSIMEGCNKYCSFCVVPYTRGEEVSRPVDDV 181
Query: 177 LDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKV-TLVDLLEEIEKIDGIERVRIGSIDP 234
L EI LAE G +E+ L G + +Y G D + + +LL + IDGI+R+R + P
Sbjct: 182 LFEIAQLAEQGVREVNLLGQNVNAYRGPTHDGGICSFAELLRLVASIDGIDRLRFTTSHP 241
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
FT+D++ +L HL +QSG D L M R +TA EY+ I+ L+ ++
Sbjct: 242 IEFTDDIIDVYADTPELVSFLHLPVQSGSDRVLNLMKRNHTALEYKSIIRKLKKVRPNIQ 301
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
I++D IVGFPG + ++ + F YS R GT A +M + V +K +R
Sbjct: 302 ISSDFIVGFPGETDQDFEDTMNLIAQVNFDMSFSFIYSARPGTPAADMPDDVTEEEKKQR 361
Query: 355 SKKLIELNRINEKA--FAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVS 412
L + RIN +A F+ +G VL E + TG T VN N+
Sbjct: 362 LYLLQQ--RINNQAAQFSRAMLGTEQRVLVEGPSKKDIMELTGRTETNRIVNFAGTPNMI 419
Query: 413 GKILNVKI-----NSFEGEVAEGE 431
GK +++KI NS GEV E
Sbjct: 420 GKFVDIKITDVYTNSLRGEVVRTE 443
>MIAB_HAES1 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 474
Score = 201 bits (510), Expect = 1e-53
Identities = 144/444 (32%), Positives = 219/444 (49%), Gaps = 18/444 (4%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVR-EGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S M + + G E+ D E ADV ++NTCS+ +K +
Sbjct: 4 KLHIKTWGCQMNEYDSSKMADLLLNTHGLELTDVPEEADVLLLNTCSIREKAQEKVFHQL 63
Query: 61 GR---ARRQNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR ++QNP +I V GC A + S VD+V G + + +N+ R GK
Sbjct: 64 GRWKELKKQNPSLVIGVGGCVASQEGEHIRSRAPYVDIVFGPQTLHRLPEMINQIRG-GK 122
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
V + + + F+ L T AF+ I +GCN++C++C++PYTRG S+ V
Sbjct: 123 SAVVDVSFPEIEKFDRLPEPRAEGPT-AFVSIMEGCNKYCSFCVVPYTRGEEVSRPVDDV 181
Query: 177 LDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKV-TLVDLLEEIEKIDGIERVRIGSIDP 234
L EI LAE G +E+ L G + +Y G D + + +LL + IDGI+R+R + P
Sbjct: 182 LFEIAQLAEQGVREVNLLGQNVNAYRGPTHDGGICSFAELLRLVASIDGIDRLRFTTSHP 241
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
FT+D++ +L HL +QSG D L M R +TA EY+ I+ L+ ++
Sbjct: 242 IEFTDDIIDVYADTPELVSFLHLPVQSGSDRVLNLMKRNHTALEYKSIIRKLKKVRPNIQ 301
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
I++D IVGFPG + ++ + F YS R GT A +M + V +K +R
Sbjct: 302 ISSDFIVGFPGETDQDFEDTMNLIAQVNFDMSFSFIYSARPGTPAADMPDDVTEEEKKQR 361
Query: 355 SKKLIELNRINEKA--FAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVS 412
L + RIN +A F+ +G VL E + TG T VN N+
Sbjct: 362 LYLLQQ--RINNQAAQFSRAMLGTEQRVLVEGPSKKDIMELTGRTETNRIVNFAGTPNMI 419
Query: 413 GKILNVKI-----NSFEGEVAEGE 431
GK +++KI NS GEV E
Sbjct: 420 GKFVDIKITDVYTNSLRGEVVRTE 443
>MIAB_CORDI RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 516
Score = 201 bits (510), Expect = 1e-53
Identities = 119/387 (30%), Positives = 194/387 (50%), Gaps = 12/387 (3%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIGR---A 63
T GC++N ++SE ++ GY V E D+ V NTC+V DK+ +G+ A
Sbjct: 23 TFGCQMNVHDSERLSGLLEEAGYHAVADGEEPDLVVFNTCAVRENADKRLYGTLGQLRSA 82
Query: 64 RRQNPEAIIAVVGCYAQI-APTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVKVG 122
+ +NP IAV GC AQ T V+ VD V G+ N G + ++++ + +V++
Sbjct: 83 KEKNPRMQIAVGGCLAQKDKDTVVAKAPWVDAVFGTHNMGALPSLLSRSEHNKRAEVEIV 142
Query: 123 AVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDEIRS 182
L+ F + + + ++ + GCN C +C++P RG + P +L E+++
Sbjct: 143 DSLEQ--FPSVLPAKRESAYAGWVSVSVGCNNTCTFCIVPSLRGKEVDRRPGDILAEVQA 200
Query: 183 LAEHGFKEIILSGIHTASYGVDLDE------KVTLVDLLEEIEKIDGIERVRIGSIDPTF 236
L + G E+ L G + +YGV+ + + LL KI+G+ER+R S P
Sbjct: 201 LVDQGVSEVTLLGQNVNAYGVNFSDPDIQRDRFAFSKLLRACGKIEGLERLRFTSPHPAE 260
Query: 237 FTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSIT 296
FT DV+ + +CP H+ LQSG D LK M R Y +K++ I+ +R KI SIT
Sbjct: 261 FTHDVIDAMAETPNVCPQLHMPLQSGSDKVLKEMRRSYRSKKFLGILEEVRAKIPHASIT 320
Query: 297 TDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSK 356
TD+IVGFPG +++ + + F+YSPR GT A + +QV +R +
Sbjct: 321 TDIIVGFPGETEEDFQETLNVVEKARFTSAYTFQYSPRPGTPAADYADQVPKEVVQDRYE 380
Query: 357 KLIELNRINEKAFAEKYIGEVIDVLFE 383
+L+ L K IG +++L +
Sbjct: 381 RLLALQERISTEENAKLIGTEVELLVQ 407
>MIAB_ACTPJ RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 475
Score = 201 bits (510), Expect = 1e-53
Identities = 143/444 (32%), Positives = 218/444 (49%), Gaps = 18/444 (4%)
Query: 2 KVAFATLGCRVNTYESEAMTEKF-VREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S M + G E+ D E ADV ++NTCS+ +K +
Sbjct: 3 KLHITTWGCQMNEYDSSKMADLLNSTHGLELTDKPEEADVLLLNTCSIREKAQEKVFSQL 62
Query: 61 GRAR---RQNPEAIIAVVGCYAQIAPTEVSG-IEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR + + P+ II V GC A + VD+V G + + +NK R +
Sbjct: 63 GRWKNWKKDKPDLIIGVGGCVASQEGEHIRDRAPFVDIVFGPQTLHRLPEMINKIRGGDR 122
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
V + K F+ L T AF+ I +GCN++C++C++PYTRG S+ V
Sbjct: 123 AIVDISFPEIEK-FDRLPEPRAEGPT-AFVSIMEGCNKYCSFCVVPYTRGEEVSRPVDDV 180
Query: 177 LDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKV-TLVDLLEEIEKIDGIERVRIGSIDP 234
L EI LAE G +E+ L G + +Y G D + T +LL + IDGI+RVR + P
Sbjct: 181 LFEIAQLAEQGVREVNLLGQNVNAYRGETFDGGICTFAELLRLVAAIDGIDRVRYTTSHP 240
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
FT+D++ +L HL +QSG D L M R +TA EY+ I+ LR+ ++
Sbjct: 241 IEFTDDIIEVYRDTPELVSFLHLPIQSGADRVLTMMKRNHTALEYKAIIRKLREVRPNIQ 300
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
I++D IVGFPG ++++ + F YS R GT A ++ + + +K R
Sbjct: 301 ISSDFIVGFPGETAEDFEQTMKVIEQVNFDMSFSFIYSARPGTPAADLPDDISEEEKKAR 360
Query: 355 SKKLIELNRINEKA--FAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVS 412
+L + RIN +A F+ +G VL E + TG T N VN + ++
Sbjct: 361 LARLQQ--RINHQAMQFSRAMLGTEQRVLVEGPSKKDIMELTGRTENNRIVNFQGTPDMI 418
Query: 413 GKILNVKI-----NSFEGEVAEGE 431
GK +++KI NS GEV E
Sbjct: 419 GKFVDIKITDVYTNSLRGEVVRTE 442
>MIAB_METEP RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 446
Score = 200 bits (509), Expect = 2e-53
Identities = 140/447 (31%), Positives = 213/447 (47%), Gaps = 23/447 (5%)
Query: 1 MKVAFA-TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQI 59
MK A+ + GC++N Y++ M + EGY D E ADV V+NTC + +K
Sbjct: 1 MKKAYVKSYGCQMNAYDAGRMADVLAAEGYSATDTVEEADVVVLNTCHIREKAAEKVYSE 60
Query: 60 IGRAR-------RQNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKA 111
+GR R + I V GC AQ E+ S VDVV+G ++ + + ++
Sbjct: 61 LGRLRVLKGERAESGHDTRIVVAGCVAQAEGREILSRAPAVDVVVGPQSYHRLPDLLRQS 120
Query: 112 RDEGKQQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSK 171
R+ + A K F+ L N FL +Q+GC++FCA+C++PYTRG+ S+
Sbjct: 121 RETRVVDTEFPAEDK---FDHLPARR-NRGVTGFLTVQEGCDKFCAFCVVPYTRGAEVSR 176
Query: 172 DPKKVLDEIRSLAEHGFKEIILSGIHTASY---GVDLDEKVTLVDLLEEIEKIDGIERVR 228
V+DE R L E G +EI L G + +Y G D TL L++ + + G+ R+R
Sbjct: 177 SVAAVVDEARRLVEGGVREITLIGQNVNAYHGNGPD-GAPATLGHLMDALSAVPGLLRLR 235
Query: 229 IGSIDPTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRD 288
+ P F +D++ + P+ HL +QSG D L MNRR+T Y ++ +R+
Sbjct: 236 YTTSHPNDFADDLIAAHATNPLVMPYLHLPVQSGSDRILHAMNRRHTGDAYRRLIERIRN 295
Query: 289 KIEDVSITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDG 348
D+++++D IVGFPG + I S FKYSPR GT A E+ V
Sbjct: 296 ARPDIALSSDFIVGFPGETDADFAETMRLVSDIGFSAAFSFKYSPRAGTPAAEREDAVPE 355
Query: 349 NKKDERSKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKAD 408
K ER L +L A+ +G + ++L E+ G G T + V A
Sbjct: 356 AVKTERLAALQDLLDRQRHAYNAASVGTLTEILV-EKTGRHPGQVAGKTPHLQAVQFDAP 414
Query: 409 CNVSGKILNVKI-----NSFEGEVAEG 430
+ G ++ V+I NS GE EG
Sbjct: 415 ASTIGTVVPVRITRAGSNSLFGETLEG 441
>MIAB_BURP8 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 456
Score = 200 bits (509), Expect = 2e-53
Identities = 138/446 (30%), Positives = 213/446 (47%), Gaps = 15/446 (3%)
Query: 2 KVAFATLGCRVNTYESEAMTEKF-VREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
KV T GC++N Y+S+ M + EG D E ADV + NTCSV +K +
Sbjct: 4 KVYVKTFGCQMNEYDSDKMVDVLGAAEGLVKTDTPEDADVILFNTCSVREKAQEKVFSDL 63
Query: 61 GRARR---QNPEAIIAVVGCYA-QIAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR R NP +I V GC A Q + V+ VD+V G + + +++ R G+
Sbjct: 64 GRVRELKEANPNLLIGVGGCVASQEGASIVARAPYVDLVFGPQTLHRLPQMIDQRRASGR 123
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
QV + K F+ L + + AF+ I +GC+++C+YC++PYTRG S+ V
Sbjct: 124 PQVDITFPEIEK-FDHLPPARIDGPS-AFVSIMEGCSKYCSYCVVPYTRGEEVSRPLDDV 181
Query: 177 LDEIRSLAEHGFKEIILSGIHTASYG----VDLDEKVTLVDLLEEIEKIDGIERVRIGSI 232
L EI LA+ G +E+ L G + +Y + E L+E + I GIER+R +
Sbjct: 182 LTEIAGLADQGVREVTLLGQNVNAYRGAMTLGATEIADFATLIEYVADIPGIERIRYTTS 241
Query: 233 DPTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIED 292
P FT+ ++ + KL H HL +Q G D L M R YT EY+ ++ LR D
Sbjct: 242 HPKEFTQRLIDTYAKVPKLVSHLHLPVQHGSDRILMAMKRGYTVLEYKSVIRKLRAIRPD 301
Query: 293 VSITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKD 352
+S++TD+IVGFPG + ++ + F YSPR GT A N+++ K
Sbjct: 302 LSLSTDMIVGFPGETEEDFDKMMQLVHDMSYDTSFSFIYSPRPGTPAANLQDDTPREVKL 361
Query: 353 ERSKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVN----AKAD 408
R + L N + ++ +G+V +L E G T N VN +
Sbjct: 362 RRLQHLQATIEENVQRISQAMVGKVERILVERPARKDPNELAGRTENNRVVNFPAPVETH 421
Query: 409 CNVSGKILNVKINSFEGEVAEGEIVL 434
+ G++++VKI GE+V+
Sbjct: 422 ARLIGQMIDVKIVHAYPHSLRGELVM 447
>MIAB_BURCJ RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 457
Score = 200 bits (509), Expect = 2e-53
Identities = 140/446 (31%), Positives = 211/446 (47%), Gaps = 15/446 (3%)
Query: 2 KVAFATLGCRVNTYESEAMTEKF-VREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
KV T GC++N Y+S+ M + EG E D E AD+ + NTCSV +K +
Sbjct: 4 KVYVKTFGCQMNEYDSDKMVDVLNAAEGLEKTDTPEDADIILFNTCSVREKAQEKVFSDL 63
Query: 61 GRARR---QNPEAIIAVVGCYA-QIAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR R P +I V GC A Q + VS VD+V G + + ++ R G+
Sbjct: 64 GRVRELKEAKPGLLIGVGGCVASQEGASIVSRAPYVDLVFGPQTLHRLPQMIDARRASGR 123
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
QV + K F+ L + AF+ I +GC+++C+YC++PYTRG S+ V
Sbjct: 124 AQVDITFPEIEK-FDHLPPARVEGPS-AFVSIMEGCSKYCSYCVVPYTRGDEVSRPLDDV 181
Query: 177 LDEIRSLAEHGFKEIILSGIHTASYGVDL----DEKVTLVDLLEEIEKIDGIERVRIGSI 232
L E+ LA+ G +E+ L G + +Y L E L+E + I GIER+R +
Sbjct: 182 LTEVAGLADQGVREVTLLGQNVNAYRGALAAGSSEIADFATLIEYVADIPGIERIRYTTS 241
Query: 233 DPTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIED 292
P FT+ ++ + KL H HL +Q G D L M R YT EY+ ++ LR D
Sbjct: 242 HPKEFTQRLIDTYAKVPKLVNHLHLPVQHGSDRILMAMKRGYTVLEYKSVIRKLRAIRPD 301
Query: 293 VSITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKD 352
+S++TD+IVGFPG + + + F YSPR GT A N+ + + K
Sbjct: 302 LSLSTDMIVGFPGETEDDFDKMMALVHEMGYDTSFSFIYSPRPGTPAANLADDTPRDVKL 361
Query: 353 ERSKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADC--- 409
+R + L N ++ +G+V +L E G T N VN A
Sbjct: 362 KRLQHLQATIEENVARISQSMVGKVERILVEGPSRKDPNELAGRTENNRVVNFPAPLASH 421
Query: 410 -NVSGKILNVKINSFEGEVAEGEIVL 434
+ G++++VKIN GE+V+
Sbjct: 422 PRLIGQMIDVKINHAYPHSLRGELVI 447
>MIAB_BURCH RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 457
Score = 200 bits (509), Expect = 2e-53
Identities = 141/446 (31%), Positives = 210/446 (47%), Gaps = 15/446 (3%)
Query: 2 KVAFATLGCRVNTYESEAMTEKF-VREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
KV T GC++N Y+S+ M + EG E D E AD+ + NTCSV +K +
Sbjct: 4 KVYVKTFGCQMNEYDSDKMVDVLNAAEGLEKTDTPEDADIILFNTCSVREKAQEKVFSDL 63
Query: 61 GRARR---QNPEAIIAVVGCYA-QIAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR R P +I V GC A Q + VS VD+V G + + ++ R G+
Sbjct: 64 GRVRELKEAKPGLLIGVGGCVASQEGASIVSRAPYVDLVFGPQTLHRLPQMIDARRASGR 123
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
QV + K F+ L + AF+ I +GC+++C+YC++PYTRG S+ V
Sbjct: 124 AQVDITFPEIEK-FDHLPPARVEGPS-AFVSIMEGCSKYCSYCVVPYTRGDEVSRPLDDV 181
Query: 177 LDEIRSLAEHGFKEIILSGIHTASYGVDLDEKVT----LVDLLEEIEKIDGIERVRIGSI 232
L E+ LA+ G +E+ L G + +Y L T L+E + I GIER+R +
Sbjct: 182 LTEVAGLADQGVREVTLLGQNVNAYRGALTAGSTDIADFATLIEYVADIPGIERIRYTTS 241
Query: 233 DPTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIED 292
P FT+ ++ + KL H HL +Q G D L M R YT EY+ ++ LR D
Sbjct: 242 HPKEFTQRLIDTYAKVPKLVSHLHLPVQHGSDRILMAMKRGYTVLEYKSVIRKLRAIRPD 301
Query: 293 VSITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKD 352
+S++TD+IVGFPG + + + F YSPR GT A N+ + K
Sbjct: 302 LSLSTDMIVGFPGETEDDFDKMMALVHEMGYDTSFSFIYSPRPGTPAANLADDTPREVKL 361
Query: 353 ERSKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADC--- 409
+R + L N ++ +G+V +L E G T N VN A
Sbjct: 362 KRLQHLQATIEENVARISQSMVGKVERILVEGPSRKDPNELAGRTENNRVVNFPAPLASH 421
Query: 410 -NVSGKILNVKINSFEGEVAEGEIVL 434
+ G++++VKIN GE+VL
Sbjct: 422 PRLIGQMIDVKINHAYPHSLRGELVL 447
>MIAB_BURCC RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 457
Score = 200 bits (509), Expect = 2e-53
Identities = 141/446 (31%), Positives = 210/446 (47%), Gaps = 15/446 (3%)
Query: 2 KVAFATLGCRVNTYESEAMTEKF-VREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
KV T GC++N Y+S+ M + EG E D E AD+ + NTCSV +K +
Sbjct: 4 KVYVKTFGCQMNEYDSDKMVDVLNAAEGLEKTDTPEDADIILFNTCSVREKAQEKVFSDL 63
Query: 61 GRARR---QNPEAIIAVVGCYA-QIAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR R P +I V GC A Q + VS VD+V G + + ++ R G+
Sbjct: 64 GRVRELKEAKPGLLIGVGGCVASQEGASIVSRAPYVDLVFGPQTLHRLPQMIDARRASGR 123
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
QV + K F+ L + AF+ I +GC+++C+YC++PYTRG S+ V
Sbjct: 124 AQVDITFPEIEK-FDHLPPARVEGPS-AFVSIMEGCSKYCSYCVVPYTRGDEVSRPLDDV 181
Query: 177 LDEIRSLAEHGFKEIILSGIHTASYGVDLDEKVT----LVDLLEEIEKIDGIERVRIGSI 232
L E+ LA+ G +E+ L G + +Y L T L+E + I GIER+R +
Sbjct: 182 LTEVAGLADQGVREVTLLGQNVNAYRGALTAGSTDIADFATLIEYVADIPGIERIRYTTS 241
Query: 233 DPTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIED 292
P FT+ ++ + KL H HL +Q G D L M R YT EY+ ++ LR D
Sbjct: 242 HPKEFTQRLIDTYAKVPKLVSHLHLPVQHGSDRILMAMKRGYTVLEYKSVIRKLRAIRPD 301
Query: 293 VSITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKD 352
+S++TD+IVGFPG + + + F YSPR GT A N+ + K
Sbjct: 302 LSLSTDMIVGFPGETEDDFDKMMALVHEMGYDTSFSFIYSPRPGTPAANLADDTPREVKL 361
Query: 353 ERSKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADC--- 409
+R + L N ++ +G+V +L E G T N VN A
Sbjct: 362 KRLQHLQATIEENVARISQSMVGKVERILVEGPSRKDPNELAGRTENNRVVNFPAPLASH 421
Query: 410 -NVSGKILNVKINSFEGEVAEGEIVL 434
+ G++++VKIN GE+VL
Sbjct: 422 PRLIGQMIDVKINHAYPHSLRGELVL 447
>MIAB_BURCA RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 457
Score = 200 bits (509), Expect = 2e-53
Identities = 141/446 (31%), Positives = 210/446 (47%), Gaps = 15/446 (3%)
Query: 2 KVAFATLGCRVNTYESEAMTEKF-VREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
KV T GC++N Y+S+ M + EG E D E AD+ + NTCSV +K +
Sbjct: 4 KVYVKTFGCQMNEYDSDKMVDVLNAAEGLEKTDTPEDADIILFNTCSVREKAQEKVFSDL 63
Query: 61 GRARR---QNPEAIIAVVGCYA-QIAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR R P +I V GC A Q + VS VD+V G + + ++ R G+
Sbjct: 64 GRVRELKEAKPGLLIGVGGCVASQEGASIVSRAPYVDLVFGPQTLHRLPQMIDARRASGR 123
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
QV + K F+ L + AF+ I +GC+++C+YC++PYTRG S+ V
Sbjct: 124 AQVDITFPEIEK-FDHLPPARVEGPS-AFVSIMEGCSKYCSYCVVPYTRGDEVSRPLDDV 181
Query: 177 LDEIRSLAEHGFKEIILSGIHTASYGVDLDEKVT----LVDLLEEIEKIDGIERVRIGSI 232
L E+ LA+ G +E+ L G + +Y L T L+E + I GIER+R +
Sbjct: 182 LTEVAGLADQGVREVTLLGQNVNAYRGALTAGSTDIADFATLIEYVADIPGIERIRYTTS 241
Query: 233 DPTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIED 292
P FT+ ++ + KL H HL +Q G D L M R YT EY+ ++ LR D
Sbjct: 242 HPKEFTQRLIDTYAKVPKLVSHLHLPVQHGSDRILMAMKRGYTVLEYKSVIRKLRAIRPD 301
Query: 293 VSITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKD 352
+S++TD+IVGFPG + + + F YSPR GT A N+ + K
Sbjct: 302 LSLSTDMIVGFPGETEDDFDKMMALVHEMGYDTSFSFIYSPRPGTPAANLADDTPREVKL 361
Query: 353 ERSKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADC--- 409
+R + L N ++ +G+V +L E G T N VN A
Sbjct: 362 KRLQHLQATIEENVARISQSMVGKVERILVEGPSRKDPNELAGRTENNRVVNFPAPLASH 421
Query: 410 -NVSGKILNVKINSFEGEVAEGEIVL 434
+ G++++VKIN GE+VL
Sbjct: 422 PRLIGQMIDVKINHAYPHSLRGELVL 447
>MIAB_BACHD RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 538
Score = 200 bits (509), Expect = 2e-53
Identities = 135/442 (30%), Positives = 214/442 (48%), Gaps = 16/442 (3%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIG 61
K T GC++N ++SE M G+E D ADV ++NTC++ + K IG
Sbjct: 96 KFLVRTYGCQMNIHDSENMAGMLKEMGFEATDETTDADVILINTCAIRENAENKVFGEIG 155
Query: 62 ---RARRQNPEAIIAVVGCYAQ---IAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEG 115
+ +R+ PE +I V GC +Q + + + +D++ G+ N + + + A G
Sbjct: 156 NLKQLKREKPELVIGVCGCMSQEEGVVNRIMQKHQHIDMIFGTHNIHRLPHLLRNALF-G 214
Query: 116 KQQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKK 175
K+ + + + E++ KT+A++ I GC++FC YC++PYTRG S+ P+
Sbjct: 215 KEMIIEVWSKEGDIVENMP-RAREGKTQAWVNIMYGCDKFCTYCIVPYTRGKERSRRPED 273
Query: 176 VLDEIRSLAEHGFKEIILSGIHTASYGVDLDEKVTLVDLLE-EIEKIDGIERVRIGSIDP 234
++ E+R LA G+KEI L G + +Y EI KID I RVR + P
Sbjct: 274 IIQEVRDLARQGYKEITLLGQNVNAYXXXXXXXXXXXXXXXXEIRKID-IPRVRFTTSHP 332
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
F + ++ + L H HL +Q G LK M R+YT ++Y ++ ++ I + S
Sbjct: 333 RDFDDHLIEVLAKGGNLVEHIHLPVQHGNSEILKLMARKYTREQYVELAQKIKRAIPNAS 392
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
TTD+IVGFP ++ I + YSPR+GT A M++ V K ER
Sbjct: 393 FTTDLIVGFPNETDEQFEDTLSLVREIEFDSAFTYIYSPREGTPAAKMKDNVPMEVKRER 452
Query: 355 SKKLIELNRINEKAFAEK---YIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNV 411
L LN + A+K Y +V++VL E E + + G TR VN K +V
Sbjct: 453 ---LARLNALVNDISAQKNLEYQDKVVEVLVEGESKKDPNILAGRTRTNRLVNFKGPKSV 509
Query: 412 SGKILNVKINSFEGEVAEGEIV 433
G I+ VK+ + GE+V
Sbjct: 510 IGDIVYVKVTEAKTWSLNGEMV 531
>MIAB_ANASP RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 454
Score = 200 bits (509), Expect = 2e-53
Identities = 129/437 (29%), Positives = 209/437 (47%), Gaps = 16/437 (3%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIGRA--- 63
T GC++N +SE M G++ + AD+ + NTC++ + ++K +GR
Sbjct: 12 TFGCQMNKADSERMAGILEDMGFKFSEDPNNADLILYNTCTIRDNAEQKVYSYLGRQAKR 71
Query: 64 RRQNPEAIIAVVGCYAQI-APTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVKVG 122
+ + P+ + V GC AQ + + +D+V+G ++ + + G Q V
Sbjct: 72 KHEQPDLTLVVAGCVAQQEGEALLRRVPELDLVMGPQHANRLKDLLESVF-AGNQVVATE 130
Query: 123 AVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDEIRS 182
AV + ED+ ++ A++ + GCN C YC++P RG S+ P V E+
Sbjct: 131 AV---HIMEDITQARRDSTVTAWVNVIYGCNERCTYCVVPNVRGVEQSRTPAAVRAEMEE 187
Query: 183 LAEHGFKEIILSGIHTASYGVDLDEKV-------TLVDLLEEIEKIDGIERVRIGSIDPT 235
L G+KEI L G + +YG DL T DLL + + G+ER+R + P
Sbjct: 188 LGRQGYKEITLLGQNIDAYGRDLPGATPEGRHLHTFTDLLYYVHDVPGVERIRFATSHPR 247
Query: 236 FFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSI 295
+FTE +++ L K+C HFH+ QSG + LK M R YT ++Y I++ +R + D SI
Sbjct: 248 YFTERLIKACAELPKVCEHFHIPFQSGDNQLLKAMARGYTQEKYRRIIDTIRRYMPDASI 307
Query: 296 TTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERS 355
+ D IVGFPG + I + + YSPR GT A ENQ+ K +R
Sbjct: 308 SADAIVGFPGETEEQFENTLKLVDDIGFDQLNTAAYSPRPGTPAALWENQLSEEVKSDRL 367
Query: 356 KKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCN-VSGK 414
++L L + +++Y+G + +VL EE+ G T K D + + G+
Sbjct: 368 QRLNHLVNVKAAERSQRYMGRIEEVLVEEQNPKDQTQVMGRTGGNRLTFFKGDIHELKGQ 427
Query: 415 ILNVKINSFEGEVAEGE 431
++ VKIN GE
Sbjct: 428 LVMVKINEVRAFSLTGE 444
>MIAB_SALPK RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 474
Score = 200 bits (508), Expect = 2e-53
Identities = 141/444 (31%), Positives = 214/444 (48%), Gaps = 18/444 (4%)
Query: 2 KVAFATLGCRVNTYESEAMTEKF-VREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S M + GY++ D E ADV ++NTCS+ +K +
Sbjct: 4 KLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVLLLNTCSIREKAQEKVFHQL 63
Query: 61 GRAR---RQNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR R +NP+ II V GC A + VD++ G + + +N R +
Sbjct: 64 GRWRLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDIIFGPQTSHRLPEMINSVRGDRS 123
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
V + K F+ L T AF+ I +GCN++C YC++PYTRG S+ +
Sbjct: 124 PVVDISFPEIEK-FDRLPEPRAEGPT-AFVSIMEGCNKYCTYCVVPYTRGEEVSRPSDDI 181
Query: 177 LDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKV-TLVDLLEEIEKIDGIERVRIGSIDP 234
L EI LA G +E+ L G + ++ G + D T DLL + IDGI+R+R + P
Sbjct: 182 LFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGTFADLLRLVAAIDGIDRIRFTTSHP 241
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
FT+D++ +L HL +QSG D L M R +TA EY+ I+ LR D+
Sbjct: 242 IEFTDDIIEVYRDTPELVSFLHLPVQSGSDRVLNLMGRTHTALEYKAIIRKLRAARPDIQ 301
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
I++D IVGFPG + + ++ F +S R GT A +M + V +K +R
Sbjct: 302 ISSDFIVGFPGETTDDFEKTMKLIADVNFDMSYSFIFSARPGTPAADMVDDVPEEEKKQR 361
Query: 355 SKKLIELNRINEKAFA--EKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVS 412
L E RIN++A A + +G +L E +G T N VN + +
Sbjct: 362 LYILQE--RINQQAMAWSRRMLGTTQRILVEGTSRKNIMELSGRTENNRVVNFEGTPEMI 419
Query: 413 GKILNVKI-----NSFEGEVAEGE 431
GK ++V+I NS G+V E
Sbjct: 420 GKFVDVEITDVYPNSLRGKVVRTE 443
>MIAB_SALPA RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 474
Score = 200 bits (508), Expect = 2e-53
Identities = 141/444 (31%), Positives = 214/444 (48%), Gaps = 18/444 (4%)
Query: 2 KVAFATLGCRVNTYESEAMTEKF-VREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S M + GY++ D E ADV ++NTCS+ +K +
Sbjct: 4 KLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVLLLNTCSIREKAQEKVFHQL 63
Query: 61 GRAR---RQNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR R +NP+ II V GC A + VD++ G + + +N R +
Sbjct: 64 GRWRLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDIIFGPQTSHRLPEMINSVRGDRS 123
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
V + K F+ L T AF+ I +GCN++C YC++PYTRG S+ +
Sbjct: 124 PVVDISFPEIEK-FDRLPEPRAEGPT-AFVSIMEGCNKYCTYCVVPYTRGEEVSRPSDDI 181
Query: 177 LDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKV-TLVDLLEEIEKIDGIERVRIGSIDP 234
L EI LA G +E+ L G + ++ G + D T DLL + IDGI+R+R + P
Sbjct: 182 LFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGTFADLLRLVAAIDGIDRIRFTTSHP 241
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
FT+D++ +L HL +QSG D L M R +TA EY+ I+ LR D+
Sbjct: 242 IEFTDDIIEVYRDTPELVSFLHLPVQSGSDRVLNLMGRTHTALEYKAIIRKLRAARPDIQ 301
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
I++D IVGFPG + + ++ F +S R GT A +M + V +K +R
Sbjct: 302 ISSDFIVGFPGETTDDFEKTMKLIADVNFDMSYSFIFSARPGTPAADMVDDVPEEEKKQR 361
Query: 355 SKKLIELNRINEKAFA--EKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVS 412
L E RIN++A A + +G +L E +G T N VN + +
Sbjct: 362 LYILQE--RINQQAMAWSRRMLGTTQRILVEGTSRKNIMELSGRTENNRVVNFEGTPEMI 419
Query: 413 GKILNVKI-----NSFEGEVAEGE 431
GK ++V+I NS G+V E
Sbjct: 420 GKFVDVEITDVYPNSLRGKVVRTE 443
>MIAB_SACEN RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 491
Score = 200 bits (508), Expect = 2e-53
Identities = 127/432 (29%), Positives = 208/432 (48%), Gaps = 17/432 (3%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIGR---A 63
T GC++N ++SE + GY + DV V NTC+V D + +G A
Sbjct: 9 TYGCQMNVHDSERLAGMLEDAGYVRAGTDVDPDVVVFNTCAVRENADNRLYGNLGHLRPA 68
Query: 64 RRQNPEAIIAVVGCYAQIAPTE-VSGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVKVG 122
+ +NP IAV GC AQ E V VDVV G+ N G + + +AR + +V++
Sbjct: 69 KDRNPGMQIAVGGCLAQKDRGEIVKRAPWVDVVFGTHNLGSLPTLLERARHNEEAEVEIL 128
Query: 123 AVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDEIRS 182
L VF + ++ + GCN C +C++P RG + P ++L E+++
Sbjct: 129 ESLD--VFPSTLPARRESAYSGWVSVSVGCNNTCTFCIVPALRGKEKDRRPGEILSEVQA 186
Query: 183 LAEHGFKEIILSGIHTASYGVDLDEKVTLVDLLEEIEKIDGIERVRIGSIDPTFFTEDVV 242
L G E+ L G + +YGV+ ++ LL +I+G+ERVR S P FT+DV+
Sbjct: 187 LVSEGVLEVTLLGQNVNAYGVEFGDRFAFGKLLRSCGEIEGLERVRFTSPHPRDFTDDVI 246
Query: 243 RRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITTDVIVG 302
+ +C H+ LQSG D LK+M R Y ++ Y DI+ +R+ + D +ITTD+IVG
Sbjct: 247 AAMAETPNVCHQLHMPLQSGSDRVLKQMRRSYRSQRYLDILRKVREAMPDAAITTDIIVG 306
Query: 303 FPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSKKLIELN 362
FPG ++ + F+YS R GT A ME Q+ +R +LIEL
Sbjct: 307 FPGETEEDFEATMQVVREARFASAFTFQYSKRPGTPAAEMEGQLPKEVVQQRYDRLIELQ 366
Query: 363 RINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSGKILN-VKIN 421
++ +G +++L E G R + + + G++++ +
Sbjct: 367 NQISWDVGKELVGRSVELLVAE----------GEGRKDTETHRLSGRARDGRLVHFTPVG 416
Query: 422 SFEGEVAEGEIV 433
+ +G+V G++V
Sbjct: 417 AVDGQVRPGDVV 428
>MIAB_NEIM0 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 442
Score = 200 bits (508), Expect = 2e-53
Identities = 143/441 (32%), Positives = 207/441 (46%), Gaps = 15/441 (3%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVRE--GYEVVDFNEMADVYVVNTCSVTNMGDKKSRQI 59
KV T GC++N Y+S+ M E G E V + AD+ + NTCSV +K
Sbjct: 3 KVFIRTFGCQMNEYDSDKMLAVLAEEHGGIEQVTQADEADIILFNTCSVREKAQEKVFSD 62
Query: 60 IGRAR---RQNPEAIIAVVGCYA-QIAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEG 115
+GR R +NP II V GC A Q + VDVV G + + + G
Sbjct: 63 LGRVRPLKEKNPGLIIGVAGCVASQEGENIIKRAPYVDVVFGPQTLHRLPKMIVDKETSG 122
Query: 116 KQQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKK 175
QV + K F+ L AF+ I +GC+++C++C++PYTRG S+
Sbjct: 123 LSQVDISFPEIEK-FDHLPPARVEGGA-AFVSIMEGCSKYCSFCVVPYTRGEEFSRPLND 180
Query: 176 VLDEIRSLAEHGFKEIILSGIHTASYGVDLD--EKVTLVDLLEEIEKIDGIERVRIGSID 233
VL EI +LA+ G KEI L G + +Y ++D E LL + +I GIER+R +
Sbjct: 181 VLTEIANLAQQGVKEINLLGQNVNAYCGEMDDGEICDFATLLRIVHEIPGIERMRFTTSH 240
Query: 234 PTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDV 293
P FT+ ++ L KL H HL +QSG D L M R YTA EY+ I+ LR D+
Sbjct: 241 PREFTDSIIECYRDLPKLVSHLHLPIQSGSDRVLSAMKRGYTALEYKSIIRKLRAIRPDL 300
Query: 294 SITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDE 353
+++D IVGFPG +K IA + +F YSPR GT A N+ + +K
Sbjct: 301 CLSSDFIVGFPGETEREFEQTLKLVKDIAFDLSFVFIYSPRPGTPAANLHDDTPHEEKVR 360
Query: 354 RSKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSG 413
R + L E+ + IG V L E + T N VN N+
Sbjct: 361 RLEALNEVIEAETARINQTMIGTVQRCLVEGISKKDPDQLQARTANNRVVNFTGTPNMIN 420
Query: 414 KILNVKIN-----SFEGEVAE 429
++++++I S G+V E
Sbjct: 421 QMIDLEITEAYTFSLRGKVVE 441
>MIAB_LYSSC RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 514
Score = 200 bits (508), Expect = 2e-53
Identities = 126/421 (29%), Positives = 215/421 (51%), Gaps = 10/421 (2%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIG---RA 63
T GC++N +++E M F++ GY + E ADV ++NTC++ + K +G +
Sbjct: 74 TYGCQMNEHDTEVMAGIFMQLGYTPTEIIEEADVVLLNTCAIRENAENKVFGELGFLLKY 133
Query: 64 RRQNPEAIIAVVGCYAQ---IAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVK 120
+R+NPE +I V GC +Q + + VD+V G+ N + + +A + V+
Sbjct: 134 KRKNPEMLIGVCGCMSQEESVVNKILRSYPHVDMVFGTHNIHRLPNILKEAYMSKEMVVE 193
Query: 121 VGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDEI 180
V + + V E+L + + +A++ I GC++FC YC++PYTRG S+ P++++ E+
Sbjct: 194 VWSK-EGDVIENLPKKRLGS-IKAWVNIMYGCDKFCTYCIVPYTRGKERSRRPEEIIAEV 251
Query: 181 RSLAEHGFKEIILSGIHTASYGVDLDE-KVTLVDLLEEIEKIDGIERVRIGSIDPTFFTE 239
R LA G++EI+L G + +YG D ++ + L DL++ + KID I R+R + P F +
Sbjct: 252 RELAAAGYQEIMLLGQNVNAYGKDFEDIEYRLGDLMDALRKID-IPRIRFTTSHPRDFDD 310
Query: 240 DVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITTDV 299
++ + L H HL +QSG + LK M R+YT + + +V ++ I +V++TTD+
Sbjct: 311 HLIEVLAKRGNLVEHIHLPVQSGSNDILKIMARKYTREHFLTLVEKIKAAIPEVTLTTDI 370
Query: 300 IVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSKKLI 359
IVG+P + + + YSPR+GT A M + V K ER ++L
Sbjct: 371 IVGYPNETEEQFEETLSLYREVGFEAAFTYIYSPREGTPAAKMVDNVPEEVKKERLQRLN 430
Query: 360 ELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSGKILNVK 419
E+ + + V GYTR VN K V G+++ VK
Sbjct: 431 EVVGEYSRKAXXXXXXXXXXXXXXGTSKRRDDVLAGYTRKNRLVNFKGPAEVIGQLVKVK 490
Query: 420 I 420
I
Sbjct: 491 I 491
>MIAB_BURVG RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 457
Score = 200 bits (508), Expect = 2e-53
Identities = 139/446 (31%), Positives = 212/446 (47%), Gaps = 15/446 (3%)
Query: 2 KVAFATLGCRVNTYESEAMTEKF-VREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
KV T GC++N Y+S+ M + EG E D E AD+ + NTCSV +K +
Sbjct: 4 KVYVKTFGCQMNEYDSDKMVDVLNAAEGLEKTDTPEDADIILFNTCSVREKAQEKVFSDL 63
Query: 61 GRARR---QNPEAIIAVVGCYA-QIAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR R P +I V GC A Q + VS VD+V G + + ++ R G+
Sbjct: 64 GRVRELKEAKPGLLIGVGGCVASQEGASIVSRAPYVDLVFGPQTLHRLPQMIDARRASGR 123
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
QV + K F+ L + AF+ I +GC+++C+YC++PYTRG S+ V
Sbjct: 124 AQVDITFPEIEK-FDHLPPARVEGPS-AFVSIMEGCSKYCSYCVVPYTRGDEVSRPLDDV 181
Query: 177 LDEIRSLAEHGFKEIILSGIHTASYGVDLD----EKVTLVDLLEEIEKIDGIERVRIGSI 232
L E+ LA+ G +E+ L G + +Y L + L+E + I GIER+R +
Sbjct: 182 LTEVAGLADQGVREVTLLGQNVNAYRGALSAGSADIADFATLIEYVADIPGIERIRYTTS 241
Query: 233 DPTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIED 292
P FT+ ++ + KL H HL +Q G D L M R YT EY+ ++ LR D
Sbjct: 242 HPKEFTQRLIDTYAKVPKLVSHLHLPVQHGSDRILMAMKRGYTVLEYKSVIRKLRAIRPD 301
Query: 293 VSITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKD 352
+S++TD+IVGFPG + ++ + F YSPR GT A N+ + K
Sbjct: 302 LSLSTDMIVGFPGETEDDFDKMMALVHEMSYDTSFSFIYSPRPGTPAANLHDDTPREVKL 361
Query: 353 ERSKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADC--- 409
+R + L N ++ +G+V +L E +G T N VN A
Sbjct: 362 KRLQHLQATIEENVARISQSMVGKVERILVEGPSRKDPNELSGRTENNRVVNFPAPLASH 421
Query: 410 -NVSGKILNVKINSFEGEVAEGEIVL 434
+ G++++VKIN GE++L
Sbjct: 422 PRLIGQMIDVKINHAYPHSLRGELLL 447
>MIAB_BURPS RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 457
Score = 200 bits (508), Expect = 2e-53
Identities = 138/446 (30%), Positives = 213/446 (47%), Gaps = 15/446 (3%)
Query: 2 KVAFATLGCRVNTYESEAMTEKF-VREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
KV T GC++N Y+S+ M + EG E D E AD+ + NTCSV +K +
Sbjct: 4 KVYVKTFGCQMNEYDSDKMVDVLNAAEGLEKTDTPEDADIILFNTCSVREKAQEKVFSDL 63
Query: 61 GRARR---QNPEAIIAVVGCYA-QIAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR R P+ +I V GC A Q + V+ VD+V G + + ++ R+ G+
Sbjct: 64 GRVRELKEAKPDLLIGVGGCVASQEGASIVARAPYVDLVFGPQTLHRLPQMIDARRESGR 123
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
QV + K F+ L + AF+ I +GC+++C+YC++PYTRG S+ V
Sbjct: 124 AQVDITFPEIEK-FDHLPPARVEGPS-AFVSIMEGCSKYCSYCVVPYTRGDEVSRPLDDV 181
Query: 177 LDEIRSLAEHGFKEIILSGIHTASY----GVDLDEKVTLVDLLEEIEKIDGIERVRIGSI 232
L E+ LA+ G +E+ L G + +Y E L+E + I GIER+R +
Sbjct: 182 LTEVAGLADQGVREVTLLGQNVNAYRGAIAAGSAEIADFATLIEYVADIPGIERIRYTTS 241
Query: 233 DPTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIED 292
P FT+ ++ + KL H HL +Q G D L M R YT EY+ ++ LR +
Sbjct: 242 HPKEFTQRLLDVYAKVPKLVDHLHLPVQHGSDRILMAMKRGYTVLEYKSVIRKLRAIRPN 301
Query: 293 VSITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKD 352
+S++TD+IVGFPG + ++ + F YSPR GT A N+ + K
Sbjct: 302 LSLSTDIIVGFPGETDADFDKTMALVHEMSYDTSFSFIYSPRPGTPAANLADDTPRELKL 361
Query: 353 ERSKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVN----AKAD 408
+R + L N ++ +G+V +L E G T N VN + A
Sbjct: 362 KRLQHLQATIEENVARISQSMLGKVERILVEGPSRKDPNELAGRTENNRVVNFPAPSAAH 421
Query: 409 CNVSGKILNVKINSFEGEVAEGEIVL 434
+ G++++VKIN GE+VL
Sbjct: 422 PRLIGQMIDVKINHAYPHSLRGELVL 447
>MIAB_BURP6 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 457
Score = 200 bits (508), Expect = 2e-53
Identities = 138/446 (30%), Positives = 213/446 (47%), Gaps = 15/446 (3%)
Query: 2 KVAFATLGCRVNTYESEAMTEKF-VREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
KV T GC++N Y+S+ M + EG E D E AD+ + NTCSV +K +
Sbjct: 4 KVYVKTFGCQMNEYDSDKMVDVLNAAEGLEKTDTPEDADIILFNTCSVREKAQEKVFSDL 63
Query: 61 GRARR---QNPEAIIAVVGCYA-QIAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR R P+ +I V GC A Q + V+ VD+V G + + ++ R+ G+
Sbjct: 64 GRVRELKEAKPDLLIGVGGCVASQEGASIVARAPYVDLVFGPQTLHRLPQMIDARRESGR 123
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
QV + K F+ L + AF+ I +GC+++C+YC++PYTRG S+ V
Sbjct: 124 AQVDITFPEIEK-FDHLPPARVEGPS-AFVSIMEGCSKYCSYCVVPYTRGDEVSRPLDDV 181
Query: 177 LDEIRSLAEHGFKEIILSGIHTASY----GVDLDEKVTLVDLLEEIEKIDGIERVRIGSI 232
L E+ LA+ G +E+ L G + +Y E L+E + I GIER+R +
Sbjct: 182 LTEVAGLADQGVREVTLLGQNVNAYRGAIAAGSAEIADFATLIEYVADIPGIERIRYTTS 241
Query: 233 DPTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIED 292
P FT+ ++ + KL H HL +Q G D L M R YT EY+ ++ LR +
Sbjct: 242 HPKEFTQRLLDVYAKVPKLVDHLHLPVQHGSDRILMAMKRGYTVLEYKSVIRKLRAIRPN 301
Query: 293 VSITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKD 352
+S++TD+IVGFPG + ++ + F YSPR GT A N+ + K
Sbjct: 302 LSLSTDIIVGFPGETDADFDKTMALVHEMSYDTSFSFIYSPRPGTPAANLADDTPRELKL 361
Query: 353 ERSKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVN----AKAD 408
+R + L N ++ +G+V +L E G T N VN + A
Sbjct: 362 KRLQHLQATIEENVARISQSMLGKVERILVEGPSRKDPNELAGRTENNRVVNFPAPSAAH 421
Query: 409 CNVSGKILNVKINSFEGEVAEGEIVL 434
+ G++++VKIN GE+VL
Sbjct: 422 PRLIGQMIDVKINHAYPHSLRGELVL 447
>MIAB_BURP0 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 457
Score = 200 bits (508), Expect = 2e-53
Identities = 138/446 (30%), Positives = 213/446 (47%), Gaps = 15/446 (3%)
Query: 2 KVAFATLGCRVNTYESEAMTEKF-VREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
KV T GC++N Y+S+ M + EG E D E AD+ + NTCSV +K +
Sbjct: 4 KVYVKTFGCQMNEYDSDKMVDVLNAAEGLEKTDTPEDADIILFNTCSVREKAQEKVFSDL 63
Query: 61 GRARR---QNPEAIIAVVGCYA-QIAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR R P+ +I V GC A Q + V+ VD+V G + + ++ R+ G+
Sbjct: 64 GRVRELKEAKPDLLIGVGGCVASQEGASIVARAPYVDLVFGPQTLHRLPQMIDARRESGR 123
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
QV + K F+ L + AF+ I +GC+++C+YC++PYTRG S+ V
Sbjct: 124 AQVDITFPEIEK-FDHLPPARVEGPS-AFVSIMEGCSKYCSYCVVPYTRGDEVSRPLDDV 181
Query: 177 LDEIRSLAEHGFKEIILSGIHTASY----GVDLDEKVTLVDLLEEIEKIDGIERVRIGSI 232
L E+ LA+ G +E+ L G + +Y E L+E + I GIER+R +
Sbjct: 182 LTEVAGLADQGVREVTLLGQNVNAYRGAIAAGSAEIADFATLIEYVADIPGIERIRYTTS 241
Query: 233 DPTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIED 292
P FT+ ++ + KL H HL +Q G D L M R YT EY+ ++ LR +
Sbjct: 242 HPKEFTQRLLDVYAKVPKLVDHLHLPVQHGSDRILMAMKRGYTVLEYKSVIRKLRAIRPN 301
Query: 293 VSITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKD 352
+S++TD+IVGFPG + ++ + F YSPR GT A N+ + K
Sbjct: 302 LSLSTDIIVGFPGETDADFDKTMALVHEMSYDTSFSFIYSPRPGTPAANLADDTPRELKL 361
Query: 353 ERSKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVN----AKAD 408
+R + L N ++ +G+V +L E G T N VN + A
Sbjct: 362 KRLQHLQATIEENVARISQSMLGKVERILVEGPSRKDPNELAGRTENNRVVNFPAPSAAH 421
Query: 409 CNVSGKILNVKINSFEGEVAEGEIVL 434
+ G++++VKIN GE+VL
Sbjct: 422 PRLIGQMIDVKINHAYPHSLRGELVL 447
>MIAB_BURMS RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 457
Score = 200 bits (508), Expect = 2e-53
Identities = 138/446 (30%), Positives = 213/446 (47%), Gaps = 15/446 (3%)
Query: 2 KVAFATLGCRVNTYESEAMTEKF-VREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
KV T GC++N Y+S+ M + EG E D E AD+ + NTCSV +K +
Sbjct: 4 KVYVKTFGCQMNEYDSDKMVDVLNAAEGLEKTDTPEDADIILFNTCSVREKAQEKVFSDL 63
Query: 61 GRARR---QNPEAIIAVVGCYA-QIAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR R P+ +I V GC A Q + V+ VD+V G + + ++ R+ G+
Sbjct: 64 GRVRELKEAKPDLLIGVGGCVASQEGASIVARAPYVDLVFGPQTLHRLPQMIDARRESGR 123
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
QV + K F+ L + AF+ I +GC+++C+YC++PYTRG S+ V
Sbjct: 124 AQVDITFPEIEK-FDHLPPARVEGPS-AFVSIMEGCSKYCSYCVVPYTRGDEVSRPLDDV 181
Query: 177 LDEIRSLAEHGFKEIILSGIHTASY----GVDLDEKVTLVDLLEEIEKIDGIERVRIGSI 232
L E+ LA+ G +E+ L G + +Y E L+E + I GIER+R +
Sbjct: 182 LTEVAGLADQGVREVTLLGQNVNAYRGAIAAGSAEIADFATLIEYVADIPGIERIRYTTS 241
Query: 233 DPTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIED 292
P FT+ ++ + KL H HL +Q G D L M R YT EY+ ++ LR +
Sbjct: 242 HPKEFTQRLLDVYAKVPKLVDHLHLPVQHGSDRILMAMKRGYTVLEYKSVIRKLRAIRPN 301
Query: 293 VSITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKD 352
+S++TD+IVGFPG + ++ + F YSPR GT A N+ + K
Sbjct: 302 LSLSTDIIVGFPGETDADFDKTMALVHEMSYDTSFSFIYSPRPGTPAANLADDTPRELKL 361
Query: 353 ERSKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVN----AKAD 408
+R + L N ++ +G+V +L E G T N VN + A
Sbjct: 362 KRLQHLQATIEENVARISQSMLGKVERILVEGPSRKDPNELAGRTENNRVVNFPAPSAAH 421
Query: 409 CNVSGKILNVKINSFEGEVAEGEIVL 434
+ G++++VKIN GE+VL
Sbjct: 422 PRLIGQMIDVKINHAYPHSLRGELVL 447
>MIAB_BURMA RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 457
Score = 200 bits (508), Expect = 2e-53
Identities = 138/446 (30%), Positives = 213/446 (47%), Gaps = 15/446 (3%)
Query: 2 KVAFATLGCRVNTYESEAMTEKF-VREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
KV T GC++N Y+S+ M + EG E D E AD+ + NTCSV +K +
Sbjct: 4 KVYVKTFGCQMNEYDSDKMVDVLNAAEGLEKTDTPEDADIILFNTCSVREKAQEKVFSDL 63
Query: 61 GRARR---QNPEAIIAVVGCYA-QIAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR R P+ +I V GC A Q + V+ VD+V G + + ++ R+ G+
Sbjct: 64 GRVRELKEAKPDLLIGVGGCVASQEGASIVARAPYVDLVFGPQTLHRLPQMIDARRESGR 123
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
QV + K F+ L + AF+ I +GC+++C+YC++PYTRG S+ V
Sbjct: 124 AQVDITFPEIEK-FDHLPPARVEGPS-AFVSIMEGCSKYCSYCVVPYTRGDEVSRPLDDV 181
Query: 177 LDEIRSLAEHGFKEIILSGIHTASY----GVDLDEKVTLVDLLEEIEKIDGIERVRIGSI 232
L E+ LA+ G +E+ L G + +Y E L+E + I GIER+R +
Sbjct: 182 LTEVAGLADQGVREVTLLGQNVNAYRGAIAAGSAEIADFATLIEYVADIPGIERIRYTTS 241
Query: 233 DPTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIED 292
P FT+ ++ + KL H HL +Q G D L M R YT EY+ ++ LR +
Sbjct: 242 HPKEFTQRLLDVYAKVPKLVDHLHLPVQHGSDRILMAMKRGYTVLEYKSVIRKLRAIRPN 301
Query: 293 VSITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKD 352
+S++TD+IVGFPG + ++ + F YSPR GT A N+ + K
Sbjct: 302 LSLSTDIIVGFPGETDADFDKTMALVHEMSYDTSFSFIYSPRPGTPAANLADDTPRELKL 361
Query: 353 ERSKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVN----AKAD 408
+R + L N ++ +G+V +L E G T N VN + A
Sbjct: 362 KRLQHLQATIEENVARISQSMLGKVERILVEGPSRKDPNELAGRTENNRVVNFPAPSAAH 421
Query: 409 CNVSGKILNVKINSFEGEVAEGEIVL 434
+ G++++VKIN GE+VL
Sbjct: 422 PRLIGQMIDVKINHAYPHSLRGELVL 447
>MIAB_BURM9 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 457
Score = 200 bits (508), Expect = 2e-53
Identities = 138/446 (30%), Positives = 213/446 (47%), Gaps = 15/446 (3%)
Query: 2 KVAFATLGCRVNTYESEAMTEKF-VREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
KV T GC++N Y+S+ M + EG E D E AD+ + NTCSV +K +
Sbjct: 4 KVYVKTFGCQMNEYDSDKMVDVLNAAEGLEKTDTPEDADIILFNTCSVREKAQEKVFSDL 63
Query: 61 GRARR---QNPEAIIAVVGCYA-QIAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR R P+ +I V GC A Q + V+ VD+V G + + ++ R+ G+
Sbjct: 64 GRVRELKEAKPDLLIGVGGCVASQEGASIVARAPYVDLVFGPQTLHRLPQMIDARRESGR 123
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
QV + K F+ L + AF+ I +GC+++C+YC++PYTRG S+ V
Sbjct: 124 AQVDITFPEIEK-FDHLPPARVEGPS-AFVSIMEGCSKYCSYCVVPYTRGDEVSRPLDDV 181
Query: 177 LDEIRSLAEHGFKEIILSGIHTASY----GVDLDEKVTLVDLLEEIEKIDGIERVRIGSI 232
L E+ LA+ G +E+ L G + +Y E L+E + I GIER+R +
Sbjct: 182 LTEVAGLADQGVREVTLLGQNVNAYRGAIAAGSAEIADFATLIEYVADIPGIERIRYTTS 241
Query: 233 DPTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIED 292
P FT+ ++ + KL H HL +Q G D L M R YT EY+ ++ LR +
Sbjct: 242 HPKEFTQRLLDVYAKVPKLVDHLHLPVQHGSDRILMAMKRGYTVLEYKSVIRKLRAIRPN 301
Query: 293 VSITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKD 352
+S++TD+IVGFPG + ++ + F YSPR GT A N+ + K
Sbjct: 302 LSLSTDIIVGFPGETDADFDKTMALVHEMSYDTSFSFIYSPRPGTPAANLADDTPRELKL 361
Query: 353 ERSKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVN----AKAD 408
+R + L N ++ +G+V +L E G T N VN + A
Sbjct: 362 KRLQHLQATIEENVARISQSMLGKVERILVEGPSRKDPNELAGRTENNRVVNFPAPSAAH 421
Query: 409 CNVSGKILNVKINSFEGEVAEGEIVL 434
+ G++++VKIN GE+VL
Sbjct: 422 PRLIGQMIDVKINHAYPHSLRGELVL 447
>MIAB_BURM7 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 457
Score = 200 bits (508), Expect = 2e-53
Identities = 138/446 (30%), Positives = 213/446 (47%), Gaps = 15/446 (3%)
Query: 2 KVAFATLGCRVNTYESEAMTEKF-VREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
KV T GC++N Y+S+ M + EG E D E AD+ + NTCSV +K +
Sbjct: 4 KVYVKTFGCQMNEYDSDKMVDVLNAAEGLEKTDTPEDADIILFNTCSVREKAQEKVFSDL 63
Query: 61 GRARR---QNPEAIIAVVGCYA-QIAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR R P+ +I V GC A Q + V+ VD+V G + + ++ R+ G+
Sbjct: 64 GRVRELKEAKPDLLIGVGGCVASQEGASIVARAPYVDLVFGPQTLHRLPQMIDARRESGR 123
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
QV + K F+ L + AF+ I +GC+++C+YC++PYTRG S+ V
Sbjct: 124 AQVDITFPEIEK-FDHLPPARVEGPS-AFVSIMEGCSKYCSYCVVPYTRGDEVSRPLDDV 181
Query: 177 LDEIRSLAEHGFKEIILSGIHTASY----GVDLDEKVTLVDLLEEIEKIDGIERVRIGSI 232
L E+ LA+ G +E+ L G + +Y E L+E + I GIER+R +
Sbjct: 182 LTEVAGLADQGVREVTLLGQNVNAYRGAIAAGSAEIADFATLIEYVADIPGIERIRYTTS 241
Query: 233 DPTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIED 292
P FT+ ++ + KL H HL +Q G D L M R YT EY+ ++ LR +
Sbjct: 242 HPKEFTQRLLDVYAKVPKLVDHLHLPVQHGSDRILMAMKRGYTVLEYKSVIRKLRAIRPN 301
Query: 293 VSITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKD 352
+S++TD+IVGFPG + ++ + F YSPR GT A N+ + K
Sbjct: 302 LSLSTDIIVGFPGETDADFDKTMALVHEMSYDTSFSFIYSPRPGTPAANLADDTPRELKL 361
Query: 353 ERSKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVN----AKAD 408
+R + L N ++ +G+V +L E G T N VN + A
Sbjct: 362 KRLQHLQATIEENVARISQSMLGKVERILVEGPSRKDPNELAGRTENNRVVNFPAPSAAH 421
Query: 409 CNVSGKILNVKINSFEGEVAEGEIVL 434
+ G++++VKIN GE+VL
Sbjct: 422 PRLIGQMIDVKINHAYPHSLRGELVL 447
>MIAB_BRUSU RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 467
Score = 200 bits (508), Expect = 2e-53
Identities = 131/435 (30%), Positives = 221/435 (50%), Gaps = 17/435 (3%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIG 61
KV T GC++N Y+S+ M + EGY D + AD+ ++NTC + +K +G
Sbjct: 24 KVFVKTYGCQMNVYDSQRMADSLAAEGYVATDTPDDADLVLLNTCHIREKASEKLYSALG 83
Query: 62 RARRQNP-------EAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARD 113
R R+ E I V GC AQ E+ VD+V+G + + + + R
Sbjct: 84 RLRKMKDARAADGKELTIGVAGCVAQAEGQEILRRAPNVDLVIGPQTYHRLPNALARVRG 143
Query: 114 EGKQQVKVGAVLKNKVFEDL----KIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVC 169
G++ V+ +++K FE L + E AFL +Q+GC++FC +C++PYTRGS
Sbjct: 144 -GEKVVETDYAIEDK-FEHLPAPRREETRKRGVSAFLTVQEGCDKFCTFCVVPYTRGSEV 201
Query: 170 SKDPKKVLDEIRSLAEHGFKEIILSGIHTASYGVDLDE--KVTLVDLLEEIEKIDGIERV 227
S+ K+++ E LA+ G +E+ L G + ++ + ++ + L +LL + +I GI R+
Sbjct: 202 SRSVKQIVAEAERLADSGVRELTLLGQNVNAWHGEGEDGREWGLGELLFRLARIPGIARL 261
Query: 228 RIGSIDPTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLR 287
R + P + ++ L++L P+ HL +QSG D LK MNRR+ A EY ++ +R
Sbjct: 262 RYTTSHPRDMDDSLIAAHRDLRQLMPYLHLPVQSGSDRILKAMNRRHKADEYLRLIERIR 321
Query: 288 DKIEDVSITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVD 347
+ D++++ D IVGFPG ++ + ++ + FKYSPR GT ++++ V+
Sbjct: 322 NVRPDMALSGDFIVGFPGETDQDFEDTMQLVRDVNYAQAYSFKYSPRPGTPGADLDDHVE 381
Query: 348 GNKKDERSKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKA 407
KDE AF + IG +DVL E+ +G G + + V
Sbjct: 382 EAVKDEXXXXXXXXXXXXXYAFQDSMIGRKMDVLLEKPGR-EAGQMVGRSPWLLPVIIDD 440
Query: 408 DCNVSGKILNVKINS 422
+ + G I++VKI S
Sbjct: 441 NKDRVGDIIHVKIVS 455
>MIAB_BRUO2 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 467
Score = 200 bits (508), Expect = 2e-53
Identities = 131/435 (30%), Positives = 221/435 (50%), Gaps = 17/435 (3%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIG 61
KV T GC++N Y+S+ M + EGY D + AD+ ++NTC + +K +G
Sbjct: 24 KVFVKTYGCQMNVYDSQRMADSLAAEGYVATDTPDDADLVLLNTCHIREKASEKLYSALG 83
Query: 62 RARRQNP-------EAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARD 113
R R+ E I V GC AQ E+ VD+V+G + + + + R
Sbjct: 84 RLRKMKDARAADGKELTIGVAGCVAQAEGQEILRRAPNVDLVIGPQTYHRLPNALARVRG 143
Query: 114 EGKQQVKVGAVLKNKVFEDL----KIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVC 169
G++ V+ +++K FE L + E AFL +Q+GC++FC +C++PYTRGS
Sbjct: 144 -GEKVVETDYAIEDK-FEHLPAPRREETRKRGVSAFLAVQEGCDKFCTFCVVPYTRGSEV 201
Query: 170 SKDPKKVLDEIRSLAEHGFKEIILSGIHTASYGVDLDE--KVTLVDLLEEIEKIDGIERV 227
S+ K+++ E LA+ G +E+ L G + ++ + ++ + L +LL + +I GI R+
Sbjct: 202 SRSVKQIVAEAERLADSGVRELTLLGQNVNAWHGEGEDGREWGLGELLFRLARIPGIARL 261
Query: 228 RIGSIDPTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLR 287
R + P + ++ L++L P+ HL +QSG D LK MNRR+ A EY ++ +R
Sbjct: 262 RYTTSHPRDMDDSLIAAHRDLRQLMPYLHLPVQSGSDRILKAMNRRHKADEYLRLIERIR 321
Query: 288 DKIEDVSITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVD 347
+ D++++ D IVGFPG ++ + ++ + FKYSPR GT ++++ V+
Sbjct: 322 NVRPDMALSGDFIVGFPGETDQDFEDTMQLVRDVNYAQAYSFKYSPRPGTPGADLDDHVE 381
Query: 348 GNKKDERSKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKA 407
KDE AF + IG +DVL E+ +G G + + V
Sbjct: 382 EAVKDEXXXXXXXXXXXXXYAFQDSMIGRKMDVLLEKPGR-EAGQMVGRSPWLLPVIIDD 440
Query: 408 DCNVSGKILNVKINS 422
+ + G I++VKI S
Sbjct: 441 NKDRVGDIIHVKIVS 455
>MIAB_BRUC2 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 467
Score = 200 bits (508), Expect = 2e-53
Identities = 131/435 (30%), Positives = 221/435 (50%), Gaps = 17/435 (3%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIG 61
KV T GC++N Y+S+ M + EGY D + AD+ ++NTC + +K +G
Sbjct: 24 KVFVKTYGCQMNVYDSQRMADSLAAEGYVATDTPDDADLVLLNTCHIREKASEKLYSALG 83
Query: 62 RARRQNP-------EAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARD 113
R R+ E I V GC AQ E+ VD+V+G + + + + R
Sbjct: 84 RLRKMKDARAADGKELTIGVAGCVAQAEGQEILRRAPNVDLVIGPQTYHRLPNALARVRG 143
Query: 114 EGKQQVKVGAVLKNKVFEDL----KIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVC 169
G++ V+ +++K FE L + E AFL +Q+GC++FC +C++PYTRGS
Sbjct: 144 -GEKVVETDYAIEDK-FEHLPAPRREETRKRGVSAFLTVQEGCDKFCTFCVVPYTRGSEV 201
Query: 170 SKDPKKVLDEIRSLAEHGFKEIILSGIHTASYGVDLDE--KVTLVDLLEEIEKIDGIERV 227
S+ K+++ E LA+ G +E+ L G + ++ + ++ + L +LL + +I GI R+
Sbjct: 202 SRSVKQIVAEAERLADSGVRELTLLGQNVNAWHGEGEDGREWGLGELLFRLARIPGIARL 261
Query: 228 RIGSIDPTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLR 287
R + P + ++ L++L P+ HL +QSG D LK MNRR+ A EY ++ +R
Sbjct: 262 RYTTSHPRDMDDSLIAAHRDLRQLMPYLHLPVQSGSDRILKAMNRRHKADEYLRLIERIR 321
Query: 288 DKIEDVSITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVD 347
+ D++++ D IVGFPG ++ + ++ + FKYSPR GT ++++ V+
Sbjct: 322 NVRPDMALSGDFIVGFPGETDQDFEDTMQLVRDVNYAQAYSFKYSPRPGTPGADLDDHVE 381
Query: 348 GNKKDERSKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKA 407
KDE AF + IG +DVL E+ +G G + + V
Sbjct: 382 EAVKDEXXXXXXXXXXXXXYAFQDSMIGRKMDVLLEKPGR-EAGQMVGRSPWLLPVIIDD 440
Query: 408 DCNVSGKILNVKINS 422
+ + G I++VKI S
Sbjct: 441 NKDRVGDIIHVKIVS 455
>MIAB_BRUAB RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 467
Score = 200 bits (508), Expect = 2e-53
Identities = 131/435 (30%), Positives = 221/435 (50%), Gaps = 17/435 (3%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIG 61
KV T GC++N Y+S+ M + EGY D + AD+ ++NTC + +K +G
Sbjct: 24 KVFVKTYGCQMNVYDSQRMADSLAAEGYVATDTPDDADLVLLNTCHIREKASEKLYSALG 83
Query: 62 RARRQNP-------EAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARD 113
R R+ E I V GC AQ E+ VD+V+G + + + + R
Sbjct: 84 RLRKMRDARAADGKELTIGVAGCVAQAEGQEILRRAPNVDLVIGPQTYHRLPNALARVRG 143
Query: 114 EGKQQVKVGAVLKNKVFEDL----KIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVC 169
G++ V+ +++K FE L + E AFL +Q+GC++FC +C++PYTRGS
Sbjct: 144 -GEKVVETDYAIEDK-FEHLPAPRREETRKRGVSAFLTVQEGCDKFCTFCVVPYTRGSEV 201
Query: 170 SKDPKKVLDEIRSLAEHGFKEIILSGIHTASYGVDLDE--KVTLVDLLEEIEKIDGIERV 227
S+ K+++ E LA+ G +E+ L G + ++ + ++ + L +LL + +I GI R+
Sbjct: 202 SRSVKQIVAEAERLADSGVRELTLLGQNVNAWHGEGEDGREWGLGELLFRLARIPGIARL 261
Query: 228 RIGSIDPTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLR 287
R + P + ++ L++L P+ HL +QSG D LK MNRR+ A EY ++ +R
Sbjct: 262 RYTTSHPRDMDDSLIAAHRDLRQLMPYLHLPVQSGSDRILKAMNRRHKADEYLRLIERIR 321
Query: 288 DKIEDVSITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVD 347
+ D++++ D IVGFPG ++ + ++ + FKYSPR GT ++++ V+
Sbjct: 322 NVRPDMALSGDFIVGFPGETDQDFEDTMQLVRDVNYAQAYSFKYSPRPGTPGADLDDHVE 381
Query: 348 GNKKDERSKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKA 407
KDE AF + IG +DVL E+ +G G + + V
Sbjct: 382 EAVKDEXXXXXXXXXXXXXYAFQDSMIGRKMDVLLEKPGR-EAGQMVGRSPWLLPVIIDD 440
Query: 408 DCNVSGKILNVKINS 422
+ + G I++VKI S
Sbjct: 441 NKDRVGDIIHVKIVS 455
>MIAB_BRUA2 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 467
Score = 200 bits (508), Expect = 2e-53
Identities = 131/435 (30%), Positives = 221/435 (50%), Gaps = 17/435 (3%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIG 61
KV T GC++N Y+S+ M + EGY D + AD+ ++NTC + +K +G
Sbjct: 24 KVFVKTYGCQMNVYDSQRMADSLAAEGYVATDTPDDADLVLLNTCHIREKASEKLYSALG 83
Query: 62 RARRQNP-------EAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARD 113
R R+ E I V GC AQ E+ VD+V+G + + + + R
Sbjct: 84 RLRKMRDARAADGKELTIGVAGCVAQAEGQEILRRAPNVDLVIGPQTYHRLPNALARVRG 143
Query: 114 EGKQQVKVGAVLKNKVFEDL----KIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVC 169
G++ V+ +++K FE L + E AFL +Q+GC++FC +C++PYTRGS
Sbjct: 144 -GEKVVETDYAIEDK-FEHLPAPRREETRKRGVSAFLTVQEGCDKFCTFCVVPYTRGSEV 201
Query: 170 SKDPKKVLDEIRSLAEHGFKEIILSGIHTASYGVDLDE--KVTLVDLLEEIEKIDGIERV 227
S+ K+++ E LA+ G +E+ L G + ++ + ++ + L +LL + +I GI R+
Sbjct: 202 SRSVKQIVAEAERLADSGVRELTLLGQNVNAWHGEGEDGREWGLGELLFRLARIPGIARL 261
Query: 228 RIGSIDPTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLR 287
R + P + ++ L++L P+ HL +QSG D LK MNRR+ A EY ++ +R
Sbjct: 262 RYTTSHPRDMDDSLIAAHRDLRQLMPYLHLPVQSGSDRILKAMNRRHKADEYLRLIERIR 321
Query: 288 DKIEDVSITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVD 347
+ D++++ D IVGFPG ++ + ++ + FKYSPR GT ++++ V+
Sbjct: 322 NVRPDMALSGDFIVGFPGETDQDFEDTMQLVRDVNYAQAYSFKYSPRPGTPGADLDDHVE 381
Query: 348 GNKKDERSKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKA 407
KDE AF + IG +DVL E+ +G G + + V
Sbjct: 382 EAVKDEXXXXXXXXXXXXXYAFQDSMIGRKMDVLLEKPGR-EAGQMVGRSPWLLPVIIDD 440
Query: 408 DCNVSGKILNVKINS 422
+ + G I++VKI S
Sbjct: 441 NKDRVGDIIHVKIVS 455
>MIAB_BRUA1 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 467
Score = 200 bits (508), Expect = 2e-53
Identities = 131/435 (30%), Positives = 221/435 (50%), Gaps = 17/435 (3%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIG 61
KV T GC++N Y+S+ M + EGY D + AD+ ++NTC + +K +G
Sbjct: 24 KVFVKTYGCQMNVYDSQRMADSLAAEGYVATDTPDDADLVLLNTCHIREKASEKLYSALG 83
Query: 62 RARRQNP-------EAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARD 113
R R+ E I V GC AQ E+ VD+V+G + + + + R
Sbjct: 84 RLRKMRDARAADGKELTIGVAGCVAQAEGQEILRRAPNVDLVIGPQTYHRLPNALARVRG 143
Query: 114 EGKQQVKVGAVLKNKVFEDL----KIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVC 169
G++ V+ +++K FE L + E AFL +Q+GC++FC +C++PYTRGS
Sbjct: 144 -GEKVVETDYAIEDK-FEHLPAPRREETRKRGVSAFLTVQEGCDKFCTFCVVPYTRGSEV 201
Query: 170 SKDPKKVLDEIRSLAEHGFKEIILSGIHTASYGVDLDE--KVTLVDLLEEIEKIDGIERV 227
S+ K+++ E LA+ G +E+ L G + ++ + ++ + L +LL + +I GI R+
Sbjct: 202 SRSVKQIVAEAERLADSGVRELTLLGQNVNAWHGEGEDGREWGLGELLFRLARIPGIARL 261
Query: 228 RIGSIDPTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLR 287
R + P + ++ L++L P+ HL +QSG D LK MNRR+ A EY ++ +R
Sbjct: 262 RYTTSHPRDMDDSLIAAHRDLRQLMPYLHLPVQSGSDRILKAMNRRHKADEYLRLIERIR 321
Query: 288 DKIEDVSITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVD 347
+ D++++ D IVGFPG ++ + ++ + FKYSPR GT ++++ V+
Sbjct: 322 NVRPDMALSGDFIVGFPGETDQDFEDTMQLVRDVNYAQAYSFKYSPRPGTPGADLDDHVE 381
Query: 348 GNKKDERSKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKA 407
KDE AF + IG +DVL E+ +G G + + V
Sbjct: 382 EAVKDEXXXXXXXXXXXXXYAFQDSMIGRKMDVLLEKPGR-EAGQMVGRSPWLLPVIIDD 440
Query: 408 DCNVSGKILNVKINS 422
+ + G I++VKI S
Sbjct: 441 NKDRVGDIIHVKIVS 455
>MIAB_THELT RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 436
Score = 199 bits (507), Expect = 3e-53
Identities = 124/400 (31%), Positives = 205/400 (51%), Gaps = 12/400 (3%)
Query: 1 MKVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
M+V F T GC++N ++E M + GYEVV+ E AD+ ++NTC V +K +
Sbjct: 1 MRVFFKTYGCQMNLNDTETMAGILSQHGYEVVNLPEEADIVILNTCVVRQKSQEKYHSAL 60
Query: 61 GR---ARRQNPEAIIAVVGCYAQIAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGKQ 117
G+ GC + + E+ G D V+GSR+ G+I + KA +
Sbjct: 61 GQFVXXXXXXXXXXXXXAGCGSNLEGEELIK-SGADFVIGSRSIGKIAEVLQKAA----R 115
Query: 118 QVKVGAVLKNKVFEDLKIEEYN-NKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
K+ + + D K +K A++ I GCNRFC YC++PYTRG S+ V
Sbjct: 116 GEKIVYLEDDICTVDSKTPRMRFSKHHAWITIIHGCNRFCTYCIVPYTRGREKSRPLPDV 175
Query: 177 LDEIRSLAEHGFKEIILSGIHTASYGVDLDEKVTLVDLLEEIEKIDGIERVRIGSIDPTF 236
L E+ LA++G KEI G + +YG DL + L L+E+ K + I+R+ + PT
Sbjct: 176 LLEVEKLAKNGVKEITFLGQNVDAYGKDLKDGTNLASLIEQAGKFEQIKRIWFLTSYPTD 235
Query: 237 FTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSIT 296
T+ ++ + K FH+ +QSG + L+ MNRRY ++ +V +R KI SI+
Sbjct: 236 ITDKLIETVAEDPKAAKSFHIPVQSGSNRILRLMNRRYDRDQFLQLVEKIRSKIPHASIS 295
Query: 297 TDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAEN-MENQVDGNKKDERS 355
+D+IVGFP +++ + ++ YSPR+GT A ++ V +K +R
Sbjct: 296 SDIIVGFPTETEYDYMQTMDLVRKARFERLNLAVYSPRQGTVASKYFKDDVPREEKVQRL 355
Query: 356 KKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTG 395
KL+EL + + +Y+G+V++++ E + + G+Y G
Sbjct: 356 NKLLELQKQINRELNMQYLGKVVEIIVEGKTK--EGLYYG 393
>MIAB_SALAR RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 474
Score = 199 bits (507), Expect = 3e-53
Identities = 141/444 (31%), Positives = 214/444 (48%), Gaps = 18/444 (4%)
Query: 2 KVAFATLGCRVNTYESEAMTEKF-VREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S M + GY++ D E ADV ++NTCS+ +K +
Sbjct: 4 KLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVLLLNTCSIREKAQEKVFHQL 63
Query: 61 GRAR---RQNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR R +NP+ II V GC A + VD++ G + + +N R +
Sbjct: 64 GRWRLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDIIFGPQTLHRLPEMINSVRGDRS 123
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
V + K F+ L T AF+ I +GCN++C YC++PYTRG S+ +
Sbjct: 124 PVVDISFPEIEK-FDRLPEPRAEGPT-AFVSIMEGCNKYCTYCVVPYTRGEEVSRPSDDI 181
Query: 177 LDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKV-TLVDLLEEIEKIDGIERVRIGSIDP 234
L EI LA G +E+ L G + ++ G + D T DLL + IDGI+R+R + P
Sbjct: 182 LFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGTFADLLRLVAAIDGIDRIRFTTSHP 241
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
FT+D++ +L HL +QSG D L M R +TA EY+ I+ LR D+
Sbjct: 242 IEFTDDIIEVYRDTPELVSFLHLPVQSGSDRVLNLMGRTHTALEYKAIIRKLRAARPDIQ 301
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
I++D IVGFPG + + ++ F +S R GT A +M + V +K +R
Sbjct: 302 ISSDFIVGFPGETSDDFEKTMKLIADVNFDMSYSFIFSARPGTPAADMVDDVPEEEKKQR 361
Query: 355 SKKLIELNRINEKAFA--EKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVS 412
L E RIN++A A + +G +L E +G T N VN + +
Sbjct: 362 LYILQE--RINQQAMAWSRRMLGSTQRILVEGTSRKNIMELSGRTENNRVVNFEGTPEMI 419
Query: 413 GKILNVKI-----NSFEGEVAEGE 431
GK ++V+I NS G+V E
Sbjct: 420 GKFVDVEITDVYPNSLRGKVVRTE 443
>MIAB_PROMA RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 466
Score = 199 bits (507), Expect = 3e-53
Identities = 130/431 (30%), Positives = 215/431 (49%), Gaps = 20/431 (4%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIGRA--- 63
T GC++N +SE M GY+ AD+ + NTC++ + ++K +GR
Sbjct: 27 TFGCQMNKADSERMAGILQAMGYQKAKTELCADLVLYNTCTIRDNAEQKVYSYLGRQAIR 86
Query: 64 RRQNPEAIIAVVGCYAQI-APTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVKVG 122
++ +P + V GC AQ + + + +D+V+G ++ + +N+ D G+Q V
Sbjct: 87 KKSSPHLKLVVAGCVAQQEGESLLRRVPELDLVMGPQHANRLEDLLNQV-DNGQQVV--- 142
Query: 123 AVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDEIRS 182
A ++ + EDL ++ A++ + GCN C YC++P RG S++PK + EI
Sbjct: 143 ATEEHLILEDLTAARRDSNICAWVNVIYGCNERCTYCVVPSVRGKEQSREPKAIKLEIED 202
Query: 183 LAEHGFKEIILSGIHTASYGVDLD-------EKVTLVDLLEEIEKIDGIERVRIGSIDPT 235
LA+ GFKE+ L G + +YG DL + TL DLL I I+GI R+R + P
Sbjct: 203 LAKKGFKEVTLLGQNIDAYGRDLPGISSSGRRENTLTDLLYFIHDINGINRIRFATSHPR 262
Query: 236 FFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSI 295
+FT ++ L KLC HFH+ QSG + LKRM R YT ++Y I++ +R+ + + SI
Sbjct: 263 YFTTRLIEACAELPKLCEHFHIPFQSGDNEVLKRMGRGYTIEKYRRIIDKIRELMPNSSI 322
Query: 296 TTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERS 355
++DVIV FPG ++ I + + YS R T A + Q+ + K +R
Sbjct: 323 SSDVIVAFPGEDESQFQNTLKIIREIGFDQVNTAAYSQRPNTPAASWAEQLPESVKIDRL 382
Query: 356 KKLIELNRINEKAFAEKYIGEVIDVLFE-----EEVELGSGVYTGYTRNYIKVNAKADCN 410
K+L L K +Y ++++VL E + +L T + K+ K
Sbjct: 383 KELNLLVEQTAKDKNTRYHNQIVEVLAEGINPKNQEQLMGRTRTNRLTFFSKIGPKKYSY 442
Query: 411 VSGKILNVKIN 421
G ++ VKI+
Sbjct: 443 NPGDLVKVKIS 453
>MIAB_JANMA RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 446
Score = 199 bits (507), Expect = 3e-53
Identities = 137/443 (30%), Positives = 217/443 (48%), Gaps = 12/443 (2%)
Query: 2 KVAFATLGCRVNTYESEAMTEKF-VREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S+ M + EG D E ADV ++NTCSV +K +
Sbjct: 4 KIYIKTFGCQMNEYDSDKMVDVLNASEGLIKTDTPEDADVILLNTCSVREKAQEKVFSDL 63
Query: 61 GRARR---QNPEAIIAVVGCYA-QIAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR R NP+ +I V GC A Q V VD+V G + + + + R G+
Sbjct: 64 GRLRELKFNNPDLVIGVGGCVASQEGDAIVKRAPYVDLVFGPQTLHRLPEMLKQRRSTGR 123
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
QV + K F+ + + T AF+ I +GC+++C+YC++PYTRG S+ + V
Sbjct: 124 SQVDISFPEIEK-FDHMPPAKVEGAT-AFVSIMEGCSKYCSYCVVPYTRGEEVSRRFEDV 181
Query: 177 LDEIRSLAEHGFKEIILSGIHTASYGVDL--DEKVTLVDLLEEIEKIDGIERVRIGSIDP 234
L E+ L G KEI L G + +Y ++ E L+E I +++GIER+R + P
Sbjct: 182 LAEVAGLEAQGVKEITLLGQNVNAYRGEMADGEIADFALLIEYIAELEGIERIRFVTSHP 241
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
FT+ ++ + KL H +L Q G D L M R YT+ EY+ I+ LR+ ++S
Sbjct: 242 KEFTQRLIDAYAKVPKLVNHLYLPAQHGSDRILAAMKRGYTSLEYKSILRRLREVRPNIS 301
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
I++D IVGFPG + I + F +SPR GT A N+E+ K +R
Sbjct: 302 ISSDFIVGFPGETDADFEAMMKLINDIGYDNSFSFIFSPRPGTPAANLEDDTPHEVKLQR 361
Query: 355 SKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKA---DCNV 411
++L + N + ++++ +G V +L E + G T N VN A +
Sbjct: 362 LQRLQAVIDQNTRRYSDEMVGTVQRILVEGPSKKDPDELQGRTENNRVVNFAAGEHGARL 421
Query: 412 SGKILNVKINSFEGEVAEGEIVL 434
G++++V I GEI++
Sbjct: 422 IGQMVDVNIVQSFAYTLRGEIIV 444
>MIAB_GLOVI RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 441
Score = 199 bits (507), Expect = 3e-53
Identities = 124/423 (29%), Positives = 214/423 (50%), Gaps = 15/423 (3%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIG---RA 63
T GC++N +SE M GY +VD ++ AD+ + NTC++ + ++K +G R
Sbjct: 16 TFGCQMNKADSERMAGALTHLGYRIVDESDTADLVLFNTCTIRDNAEQKVYSYLGQQARR 75
Query: 64 RRQNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVKVG 122
++++P + + GC AQ ++ + +D+V+G ++ + + + EG+Q V
Sbjct: 76 KQRDPHITLVLAGCVAQQEGEKLLRRVPELDLVMGPQHVNRLSDLLERVA-EGEQVVATE 134
Query: 123 AVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDEIRS 182
+ ++ ED+ ++ A+ I GCN C YC++P RG S+ P+ + EI
Sbjct: 135 PI---EILEDITKPRRDSAVTAWANIIYGCNEGCTYCIVPSVRGREQSRTPEAIKAEICE 191
Query: 183 LAEHGFKEIILSGIHTASYGVDLDEKVTLVDLLEEIEKIDGIERVRIGSIDPTFFTEDVV 242
L G+KE+ L G + +YG D+ L LL I GIER+R + P +F+++++
Sbjct: 192 LGASGYKEVTLLGQNIDAYGRDIG--TNLASLLRFIHNAPGIERLRFATSHPRYFSDELI 249
Query: 243 RRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITTDVIVG 302
L K+C HFH+ QSG + L+RM R YT ++Y I+ +R + D +I+ D+IVG
Sbjct: 250 ATCAELPKVCEHFHIPFQSGDNEVLRRMARGYTHEKYRAIIEKIRAILPDAAISADLIVG 309
Query: 303 FPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSKKLIELN 362
FPG + + + YSPR GT A Q+D K +R ++ L
Sbjct: 310 FPGETEAQFENSLRLVDELEFDALNTAAYSPRPGTPAARWPGQLDDEVKQDRLQRTNRL- 368
Query: 363 RINEKAF--AEKYIGEVIDVLFEEEVELGSGVYTGYTR-NYIKVNAKADCNVSGKILNVK 419
+ +KAF +++Y+G VL EE G TR N + A + G++++V+
Sbjct: 369 -VAQKAFERSQRYLGRTEQVLVEETNPRDRSQVVGRTRTNRLVFFAGELAKLRGQLVDVQ 427
Query: 420 INS 422
I +
Sbjct: 428 ITA 430
>MIAB_CYAP7 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 454
Score = 199 bits (507), Expect = 3e-53
Identities = 130/439 (29%), Positives = 212/439 (48%), Gaps = 16/439 (3%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIGRA--- 63
T GC++N +SE M GY+ D AD+ + NTC++ + ++K +GR
Sbjct: 12 TFGCQMNKADSERMAGILEDIGYQWSDDPNEADLVLYNTCTIRDNAEQKVYSYLGRQAKR 71
Query: 64 RRQNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVKVG 122
+ + P+ + V GC AQ ++ + +D+V+G ++ + + + G Q V
Sbjct: 72 KHEKPDLTLIVAGCVAQQEGEKLLRRVPELDLVMGPQHANRLQDLLQEVL-AGNQVVATE 130
Query: 123 AVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDEIRS 182
+ + ED+ + A++ + GCN C YC++P RG S+ P+ + E+
Sbjct: 131 PI---HIVEDITKPRRESTVTAWVNVIYGCNERCTYCVVPNVRGVEQSRTPQAIRAEMVE 187
Query: 183 LAEHGFKEIILSGIHTASYGVDLD-------EKVTLVDLLEEIEKIDGIERVRIGSIDPT 235
L + G+KEI L G + +YG DL + TL DLL + + GIER+R + P
Sbjct: 188 LGQQGYKEITLLGQNIDAYGRDLPGVTESGRHQHTLTDLLYYVNDVPGIERIRFATSHPR 247
Query: 236 FFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSI 295
+FTE ++ L K+C HFH+ QSG + LK M R YT + Y I++ +R+ + D SI
Sbjct: 248 YFTERLIVACKELPKVCEHFHIPFQSGDNDLLKAMKRGYTHERYRQIIDKIREYMPDASI 307
Query: 296 TTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERS 355
+ D IVGFPG ++ I + + YSPR GT A +NQ+ K +R
Sbjct: 308 SADAIVGFPGETEAQFENTLKLVEDIGFDQLNTAAYSPRPGTPAALWDNQLSEEVKSDRL 367
Query: 356 KKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCN-VSGK 414
++L L I +++Y+G + +VL E+ G TR D N + G+
Sbjct: 368 QRLNHLVAIKAAERSQRYLGRIEEVLVEDVNPKDLTQVMGRTRGNRLTFFTGDINQLKGQ 427
Query: 415 ILNVKINSFEGEVAEGEIV 433
++ VKI GE V
Sbjct: 428 LVEVKITDVRAFSLTGEPV 446
>MIAB_CORGL RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 522
Score = 199 bits (507), Expect = 3e-53
Identities = 130/438 (29%), Positives = 211/438 (48%), Gaps = 23/438 (5%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIGRAR-- 64
T GC++N ++SE ++ GY + D+ V NTC+V D + +G R
Sbjct: 30 TYGCQMNVHDSERLSGLLEEAGYVAAPEDTTPDLVVFNTCAVRENADMRLYGTLGNLRSV 89
Query: 65 -RQNPEAIIAVVGCYAQI-APTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVKVG 122
+NP IAV GC AQ T V VDVV G+ N G + + +A + +V++
Sbjct: 90 KEKNPGMQIAVGGCLAQKDKDTVVKKAPWVDVVFGTHNIGSLPTLLQRAEHNAQAEVEIV 149
Query: 123 AVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDEIRS 182
L+ F + + + ++ + GCN C +C++P RG + P +L E+++
Sbjct: 150 DSLEQ--FPSVLSAKRESAYAGWVSVSVGCNNTCTFCIVPSLRGKEQDRRPGDILAEVQA 207
Query: 183 LAEHGFKEIILSGIHTASYGVDL------DEKVTLVDLLEEIEKIDGIERVRIGSIDPTF 236
L + G E+ L G + +YGV+ ++ LL +I+G+ERVR S P
Sbjct: 208 LVDQGVTEVTLLGQNVNAYGVNFVDPELERDRSAFSKLLRACGEIEGLERVRFTSPHPAE 267
Query: 237 FTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSIT 296
FT DV+ + +CP H+ LQSG D LK M R Y +K++ I++ +R KI SIT
Sbjct: 268 FTSDVIDAMAETPNICPQLHMPLQSGSDKVLKEMRRSYRSKKFLSILDEVRAKIPHASIT 327
Query: 297 TDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSK 356
TD+IVGFPG +K+ + + F+YSPR GT A ENQ+ ER +
Sbjct: 328 TDIIVGFPGETEEDFQATLDVVKKARFTSAYTFQYSPRPGTPAAEYENQLPKEVVQERYE 387
Query: 357 KLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSGKIL 416
+L+ K IG +++L V+ G G T+ +++ +A G+++
Sbjct: 388 RLMXXXXXXXXXXXXKLIGTTVELL----VQAGGGRKNDATK---RMSGRAR---DGRLV 437
Query: 417 N-VKINSFEGEVAEGEIV 433
+ +GE+ G+ V
Sbjct: 438 HFAPEGDIDGEIRPGDFV 455
>MIAB_CHRVO RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 444
Score = 199 bits (507), Expect = 3e-53
Identities = 140/442 (31%), Positives = 212/442 (47%), Gaps = 14/442 (3%)
Query: 2 KVAFATLGCRVNTYESEAMTEKF-VREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
KV T GC++N Y+S+ M + EG D E ADV + NTCSV +K +
Sbjct: 3 KVYIKTFGCQMNEYDSDKMADVLGSAEGMVKTDNPEEADVILFNTCSVREKAQEKVFSDL 62
Query: 61 GRAR---RQNPEAIIAVVGCYA-QIAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR R NP+ II V GC A Q V VDVV G + + + + G+
Sbjct: 63 GRIRPLKEANPDLIIGVGGCVASQEGDAIVKRAPFVDVVFGPQTLHRLPDLIESRKQSGR 122
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
QV + K F+ + + + AF+ I +GC+++C++C++PYTRG S+ + V
Sbjct: 123 SQVDISFPEIEK-FDHIPPAKVDGGA-AFVSIMEGCSKYCSFCVVPYTRGEEVSRPFEDV 180
Query: 177 LDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKVT-LVDLLEEIEKIDGIERVRIGSIDP 234
L EI LA G KEI L G + +Y G+ D ++ LLE + ++ G+ER+R + P
Sbjct: 181 LTEIAGLAAQGVKEITLLGQNVNAYRGLMSDGEIADFALLLEYVHEVPGVERIRFTTSHP 240
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
F++ ++ L KL H HL +QSG D L M R YT EY+ I+ LR D+
Sbjct: 241 REFSQRIIDCYAKLPKLVSHLHLPVQSGSDRVLMAMKRGYTGLEYKSIIRKLRAIRPDLC 300
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
+++D I+GFPG ++ + +F YSPR GT A N+ + +K R
Sbjct: 301 LSSDFIIGFPGETEADFEQTLKLVRDCEFDFSFVFIYSPRPGTPAANLPDDTPHAEKVRR 360
Query: 355 SKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSGK 414
+ L E+ A + +G V VL E + + + T N VN + G+
Sbjct: 361 LEALNEVIEAKGYAINQSMVGTVQRVLVENVSKKDATMLAARTANNRVVNFAGHPRLLGR 420
Query: 415 ILNVKI-----NSFEGEVAEGE 431
++ VKI +S GE E
Sbjct: 421 MIEVKITAAFPHSLAGEALTSE 442
>MIAB_BRUSI RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 467
Score = 199 bits (507), Expect = 3e-53
Identities = 131/435 (30%), Positives = 221/435 (50%), Gaps = 17/435 (3%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIG 61
KV T GC++N Y+S+ M + EGY D + AD+ ++NTC + +K +G
Sbjct: 24 KVFVKTYGCQMNVYDSQRMADSLAAEGYVATDTPDDADLVLLNTCHIREKASEKLYSALG 83
Query: 62 RARRQNP-------EAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARD 113
R R+ E I V GC AQ E+ VD+V+G + + + + R
Sbjct: 84 RLRKMKDARAADGKELTIGVAGCVAQAEGQEILRRAPNVDLVIGPQTYHRLPNALARVRG 143
Query: 114 EGKQQVKVGAVLKNKVFEDL----KIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVC 169
G++ V+ +++K FE L + E AFL +Q+GC++FC +C++PYTRGS
Sbjct: 144 -GEKVVETDYAIEDK-FEHLPAPRREETRKRGVSAFLTVQEGCDKFCTFCVVPYTRGSEV 201
Query: 170 SKDPKKVLDEIRSLAEHGFKEIILSGIHTASYGVDLDE--KVTLVDLLEEIEKIDGIERV 227
S+ K+++ E LA+ G +E+ L G + ++ + ++ + L +LL + +I GI R+
Sbjct: 202 SRSVKQIVAEAERLADSGVRELTLLGQNVNAWHGEGEDGREWGLGELLFRLARIPGIARL 261
Query: 228 RIGSIDPTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLR 287
R + P + ++ L++L P+ HL +QSG D LK MNRR+ A EY ++ +R
Sbjct: 262 RYTTSHPRDMDDSLIAAHRDLRQLMPYLHLPVQSGSDRILKAMNRRHKADEYLRLIERIR 321
Query: 288 DKIEDVSITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVD 347
+ D++++ D IVGFPG ++ + ++ + FKYSPR GT ++++ V+
Sbjct: 322 NVRPDMALSGDFIVGFPGETDQDFEDTMQLVRDVNYAQAYSFKYSPRLGTPGADLDDHVE 381
Query: 348 GNKKDERSKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKA 407
KDE AF + IG +DVL E+ +G G + + V
Sbjct: 382 EAVKDEXXXXXXXXXXXXXYAFQDSMIGRKMDVLLEKPGR-EAGQMVGRSPWLLPVIIDD 440
Query: 408 DCNVSGKILNVKINS 422
+ + G I++VKI S
Sbjct: 441 NKDRVGDIIHVKIVS 455
>MIAB_SALTY RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 474
Score = 199 bits (506), Expect = 4e-53
Identities = 141/444 (31%), Positives = 214/444 (48%), Gaps = 18/444 (4%)
Query: 2 KVAFATLGCRVNTYESEAMTEKF-VREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S M + GY++ D E ADV ++NTCS+ +K +
Sbjct: 4 KLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVLLLNTCSIREKAQEKVFHQL 63
Query: 61 GRAR---RQNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR R +NP+ II V GC A + VD++ G + + +N R +
Sbjct: 64 GRWRLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDIIFGPQTLHRLPEMINSVRGDRS 123
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
V + K F+ L T AF+ I +GCN++C YC++PYTRG S+ +
Sbjct: 124 PVVDISFPEIEK-FDRLPEPRAEGPT-AFVSIMEGCNKYCTYCVVPYTRGEEVSRPSDDI 181
Query: 177 LDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKV-TLVDLLEEIEKIDGIERVRIGSIDP 234
L EI LA G +E+ L G + ++ G + D T DLL + IDGI+R+R + P
Sbjct: 182 LFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGTFADLLRLVAAIDGIDRIRFTTSHP 241
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
FT+D++ +L HL +QSG D L M R +TA EY+ I+ LR D+
Sbjct: 242 IEFTDDIIEVYRDTPELVSFLHLPVQSGSDRVLNLMGRTHTALEYKAIIRKLRAARPDIQ 301
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
I++D IVGFPG + + ++ F +S R GT A +M + V +K +R
Sbjct: 302 ISSDFIVGFPGETTDDFEKTMKLIADVNFDMSYSFIFSARPGTPAADMVDDVPEEEKKQR 361
Query: 355 SKKLIELNRINEKAFA--EKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVS 412
L E RIN++A A + +G +L E +G T N VN + +
Sbjct: 362 LYILQE--RINQQAMAWSRRMLGTTQRILVEGTSRKNIMELSGRTENNRVVNFEGTPEMI 419
Query: 413 GKILNVKI-----NSFEGEVAEGE 431
GK ++V+I NS G+V E
Sbjct: 420 GKFVDVEITDVYPNSLRGKVVRTE 443
>MIAB_SALTI RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 474
Score = 199 bits (506), Expect = 4e-53
Identities = 141/444 (31%), Positives = 214/444 (48%), Gaps = 18/444 (4%)
Query: 2 KVAFATLGCRVNTYESEAMTEKF-VREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S M + GY++ D E ADV ++NTCS+ +K +
Sbjct: 4 KLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVLLLNTCSIREKAQEKVFHQL 63
Query: 61 GRAR---RQNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR R +NP+ II V GC A + VD++ G + + +N R +
Sbjct: 64 GRWRLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDIIFGPQTLHRLPEMINSVRGDRS 123
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
V + K F+ L T AF+ I +GCN++C YC++PYTRG S+ +
Sbjct: 124 PVVDISFPEIEK-FDRLPEPRAEGPT-AFVSIMEGCNKYCTYCVVPYTRGEEVSRPSDDI 181
Query: 177 LDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKV-TLVDLLEEIEKIDGIERVRIGSIDP 234
L EI LA G +E+ L G + ++ G + D T DLL + IDGI+R+R + P
Sbjct: 182 LFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGTFADLLRLVAAIDGIDRIRFTTSHP 241
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
FT+D++ +L HL +QSG D L M R +TA EY+ I+ LR D+
Sbjct: 242 IEFTDDIIEVYRDTPELVSFLHLPVQSGSDRVLNLMGRTHTALEYKAIIRKLRAARPDIQ 301
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
I++D IVGFPG + + ++ F +S R GT A +M + V +K +R
Sbjct: 302 ISSDFIVGFPGETTDDFEKTMKLIADVNFDMSYSFIFSARPGTPAADMVDDVPEEEKKQR 361
Query: 355 SKKLIELNRINEKAFA--EKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVS 412
L E RIN++A A + +G +L E +G T N VN + +
Sbjct: 362 LYILQE--RINQQAMAWSRRMLGTTQRILVEGTSRKNIMELSGRTENNRVVNFEGTPEMI 419
Query: 413 GKILNVKI-----NSFEGEVAEGE 431
GK ++V+I NS G+V E
Sbjct: 420 GKFVDVEITDVYPNSLRGKVVRTE 443
>MIAB_SALSV RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 474
Score = 199 bits (506), Expect = 4e-53
Identities = 141/444 (31%), Positives = 214/444 (48%), Gaps = 18/444 (4%)
Query: 2 KVAFATLGCRVNTYESEAMTEKF-VREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S M + GY++ D E ADV ++NTCS+ +K +
Sbjct: 4 KLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVLLLNTCSIREKAQEKVFHQL 63
Query: 61 GRAR---RQNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR R +NP+ II V GC A + VD++ G + + +N R +
Sbjct: 64 GRWRLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDIIFGPQTLHRLPEMINSVRGDRS 123
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
V + K F+ L T AF+ I +GCN++C YC++PYTRG S+ +
Sbjct: 124 PVVDISFPEIEK-FDRLPEPRAEGPT-AFVSIMEGCNKYCTYCVVPYTRGEEVSRPSDDI 181
Query: 177 LDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKV-TLVDLLEEIEKIDGIERVRIGSIDP 234
L EI LA G +E+ L G + ++ G + D T DLL + IDGI+R+R + P
Sbjct: 182 LFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGTFADLLRLVAAIDGIDRIRFTTSHP 241
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
FT+D++ +L HL +QSG D L M R +TA EY+ I+ LR D+
Sbjct: 242 IEFTDDIIEVYRDTPELVSFLHLPVQSGSDRVLNLMGRTHTALEYKAIIRKLRAARPDIQ 301
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
I++D IVGFPG + + ++ F +S R GT A +M + V +K +R
Sbjct: 302 ISSDFIVGFPGETTDDFEKTMKLIADVNFDMSYSFIFSARPGTPAADMVDDVPEEEKKQR 361
Query: 355 SKKLIELNRINEKAFA--EKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVS 412
L E RIN++A A + +G +L E +G T N VN + +
Sbjct: 362 LYILQE--RINQQAMAWSRRMLGTTQRILVEGTSRKNIMELSGRTENNRVVNFEGTPEMI 419
Query: 413 GKILNVKI-----NSFEGEVAEGE 431
GK ++V+I NS G+V E
Sbjct: 420 GKFVDVEITDVYPNSLRGKVVRTE 443
>MIAB_SALPB RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 474
Score = 199 bits (506), Expect = 4e-53
Identities = 141/444 (31%), Positives = 214/444 (48%), Gaps = 18/444 (4%)
Query: 2 KVAFATLGCRVNTYESEAMTEKF-VREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S M + GY++ D E ADV ++NTCS+ +K +
Sbjct: 4 KLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVLLLNTCSIREKAQEKVFHQL 63
Query: 61 GRAR---RQNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR R +NP+ II V GC A + VD++ G + + +N R +
Sbjct: 64 GRWRLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDIIFGPQTLHRLPEMINSVRGDRS 123
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
V + K F+ L T AF+ I +GCN++C YC++PYTRG S+ +
Sbjct: 124 PVVDISFPEIEK-FDRLPEPRAEGPT-AFVSIMEGCNKYCTYCVVPYTRGEEVSRPSDDI 181
Query: 177 LDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKV-TLVDLLEEIEKIDGIERVRIGSIDP 234
L EI LA G +E+ L G + ++ G + D T DLL + IDGI+R+R + P
Sbjct: 182 LFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGTFADLLRLVAAIDGIDRIRFTTSHP 241
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
FT+D++ +L HL +QSG D L M R +TA EY+ I+ LR D+
Sbjct: 242 IEFTDDIIEVYRDTPELVSFLHLPVQSGSDRVLNLMGRTHTALEYKAIIRKLRAARPDIQ 301
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
I++D IVGFPG + + ++ F +S R GT A +M + V +K +R
Sbjct: 302 ISSDFIVGFPGETTDDFEKTMKLIADVNFDMSYSFIFSARPGTPAADMVDDVPEEEKKQR 361
Query: 355 SKKLIELNRINEKAFA--EKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVS 412
L E RIN++A A + +G +L E +G T N VN + +
Sbjct: 362 LYILQE--RINQQAMAWSRRMLGTTQRILVEGTSRKNIMELSGRTENNRVVNFEGTPEMI 419
Query: 413 GKILNVKI-----NSFEGEVAEGE 431
GK ++V+I NS G+V E
Sbjct: 420 GKFVDVEITDVYPNSLRGKVVRTE 443
>MIAB_SALNS RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 474
Score = 199 bits (506), Expect = 4e-53
Identities = 141/444 (31%), Positives = 214/444 (48%), Gaps = 18/444 (4%)
Query: 2 KVAFATLGCRVNTYESEAMTEKF-VREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S M + GY++ D E ADV ++NTCS+ +K +
Sbjct: 4 KLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVLLLNTCSIREKAQEKVFHQL 63
Query: 61 GRAR---RQNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR R +NP+ II V GC A + VD++ G + + +N R +
Sbjct: 64 GRWRLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDIIFGPQTLHRLPEMINSVRGDRS 123
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
V + K F+ L T AF+ I +GCN++C YC++PYTRG S+ +
Sbjct: 124 PVVDISFPEIEK-FDRLPEPRAEGPT-AFVSIMEGCNKYCTYCVVPYTRGEEVSRPSDDI 181
Query: 177 LDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKV-TLVDLLEEIEKIDGIERVRIGSIDP 234
L EI LA G +E+ L G + ++ G + D T DLL + IDGI+R+R + P
Sbjct: 182 LFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGTFADLLRLVAAIDGIDRIRFTTSHP 241
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
FT+D++ +L HL +QSG D L M R +TA EY+ I+ LR D+
Sbjct: 242 IEFTDDIIEVYRDTPELVSFLHLPVQSGSDRVLNLMGRTHTALEYKAIIRKLRAARPDIQ 301
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
I++D IVGFPG + + ++ F +S R GT A +M + V +K +R
Sbjct: 302 ISSDFIVGFPGETTDDFEKTMKLIADVNFDMSYSFIFSARPGTPAADMVDDVPEEEKKQR 361
Query: 355 SKKLIELNRINEKAFA--EKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVS 412
L E RIN++A A + +G +L E +G T N VN + +
Sbjct: 362 LYILQE--RINQQAMAWSRRMLGTTQRILVEGTSRKNIMELSGRTENNRVVNFEGTPEMI 419
Query: 413 GKILNVKI-----NSFEGEVAEGE 431
GK ++V+I NS G+V E
Sbjct: 420 GKFVDVEITDVYPNSLRGKVVRTE 443
>MIAB_SALHS RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 474
Score = 199 bits (506), Expect = 4e-53
Identities = 141/444 (31%), Positives = 214/444 (48%), Gaps = 18/444 (4%)
Query: 2 KVAFATLGCRVNTYESEAMTEKF-VREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S M + GY++ D E ADV ++NTCS+ +K +
Sbjct: 4 KLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVLLLNTCSIREKAQEKVFHQL 63
Query: 61 GRAR---RQNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR R +NP+ II V GC A + VD++ G + + +N R +
Sbjct: 64 GRWRLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDIIFGPQTLHRLPEMINSVRGDRS 123
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
V + K F+ L T AF+ I +GCN++C YC++PYTRG S+ +
Sbjct: 124 PVVDISFPEIEK-FDRLPEPRAEGPT-AFVSIMEGCNKYCTYCVVPYTRGEEVSRPSDDI 181
Query: 177 LDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKV-TLVDLLEEIEKIDGIERVRIGSIDP 234
L EI LA G +E+ L G + ++ G + D T DLL + IDGI+R+R + P
Sbjct: 182 LFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGTFADLLRLVAAIDGIDRIRFTTSHP 241
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
FT+D++ +L HL +QSG D L M R +TA EY+ I+ LR D+
Sbjct: 242 IEFTDDIIEVYRDTPELVSFLHLPVQSGSDRVLNLMGRTHTALEYKAIIRKLRAARPDIQ 301
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
I++D IVGFPG + + ++ F +S R GT A +M + V +K +R
Sbjct: 302 ISSDFIVGFPGETTDDFEKTMKLIADVNFDMSYSFIFSARPGTPAADMVDDVPEEEKKQR 361
Query: 355 SKKLIELNRINEKAFA--EKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVS 412
L E RIN++A A + +G +L E +G T N VN + +
Sbjct: 362 LYILQE--RINQQAMAWSRRMLGTTQRILVEGTSRKNIMELSGRTENNRVVNFEGTPEMI 419
Query: 413 GKILNVKI-----NSFEGEVAEGE 431
GK ++V+I NS G+V E
Sbjct: 420 GKFVDVEITDVYPNSLRGKVVRTE 443
>MIAB_SALG2 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 474
Score = 199 bits (506), Expect = 4e-53
Identities = 141/444 (31%), Positives = 214/444 (48%), Gaps = 18/444 (4%)
Query: 2 KVAFATLGCRVNTYESEAMTEKF-VREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S M + GY++ D E ADV ++NTCS+ +K +
Sbjct: 4 KLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVLLLNTCSIREKAQEKVFHQL 63
Query: 61 GRAR---RQNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR R +NP+ II V GC A + VD++ G + + +N R +
Sbjct: 64 GRWRLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDIIFGPQTLHRLPEMINSVRGDRS 123
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
V + K F+ L T AF+ I +GCN++C YC++PYTRG S+ +
Sbjct: 124 PVVDISFPEIEK-FDRLPEPRAEGPT-AFVSIMEGCNKYCTYCVVPYTRGEEVSRPSDDI 181
Query: 177 LDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKV-TLVDLLEEIEKIDGIERVRIGSIDP 234
L EI LA G +E+ L G + ++ G + D T DLL + IDGI+R+R + P
Sbjct: 182 LFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGTFADLLRLVAAIDGIDRIRFTTSHP 241
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
FT+D++ +L HL +QSG D L M R +TA EY+ I+ LR D+
Sbjct: 242 IEFTDDIIEVYRDTPELVSFLHLPVQSGSDRVLNLMGRTHTALEYKAIIRKLRAARPDIQ 301
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
I++D IVGFPG + + ++ F +S R GT A +M + V +K +R
Sbjct: 302 ISSDFIVGFPGETNDDFEKTMKLIADVNFDMSYSFIFSARPGTPAADMVDDVPEEEKKQR 361
Query: 355 SKKLIELNRINEKAFA--EKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVS 412
L E RIN++A A + +G +L E +G T N VN + +
Sbjct: 362 LYILQE--RINQQAMAWSRRMLGTTQRILVEGTSRKNIMELSGRTENNRVVNFEGTPEMI 419
Query: 413 GKILNVKI-----NSFEGEVAEGE 431
GK ++V+I NS G+V E
Sbjct: 420 GKFVDVEITDVYPNSLRGKVVRTE 443
>MIAB_SALEP RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 474
Score = 199 bits (506), Expect = 4e-53
Identities = 141/444 (31%), Positives = 214/444 (48%), Gaps = 18/444 (4%)
Query: 2 KVAFATLGCRVNTYESEAMTEKF-VREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S M + GY++ D E ADV ++NTCS+ +K +
Sbjct: 4 KLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVLLLNTCSIREKAQEKVFHQL 63
Query: 61 GRAR---RQNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR R +NP+ II V GC A + VD++ G + + +N R +
Sbjct: 64 GRWRLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDIIFGPQTLHRLPEMINSVRGDRS 123
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
V + K F+ L T AF+ I +GCN++C YC++PYTRG S+ +
Sbjct: 124 PVVDISFPEIEK-FDRLPEPRAEGPT-AFVSIMEGCNKYCTYCVVPYTRGEEVSRPSDDI 181
Query: 177 LDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKV-TLVDLLEEIEKIDGIERVRIGSIDP 234
L EI LA G +E+ L G + ++ G + D T DLL + IDGI+R+R + P
Sbjct: 182 LFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGTFADLLRLVAAIDGIDRIRFTTSHP 241
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
FT+D++ +L HL +QSG D L M R +TA EY+ I+ LR D+
Sbjct: 242 IEFTDDIIEVYRDTPELVSFLHLPVQSGSDRVLNLMGRTHTALEYKAIIRKLRAARPDIQ 301
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
I++D IVGFPG + + ++ F +S R GT A +M + V +K +R
Sbjct: 302 ISSDFIVGFPGETNDDFEKTMKLIADVNFDMSYSFIFSARPGTPAADMVDDVPEEEKKQR 361
Query: 355 SKKLIELNRINEKAFA--EKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVS 412
L E RIN++A A + +G +L E +G T N VN + +
Sbjct: 362 LYILQE--RINQQAMAWSRRMLGTTQRILVEGTSRKNIMELSGRTENNRVVNFEGTPEMI 419
Query: 413 GKILNVKI-----NSFEGEVAEGE 431
GK ++V+I NS G+V E
Sbjct: 420 GKFVDVEITDVYPNSLRGKVVRTE 443
>MIAB_SALDC RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 474
Score = 199 bits (506), Expect = 4e-53
Identities = 141/444 (31%), Positives = 214/444 (48%), Gaps = 18/444 (4%)
Query: 2 KVAFATLGCRVNTYESEAMTEKF-VREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S M + GY++ D E ADV ++NTCS+ +K +
Sbjct: 4 KLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVLLLNTCSIREKAQEKVFHQL 63
Query: 61 GRAR---RQNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR R +NP+ II V GC A + VD++ G + + +N R +
Sbjct: 64 GRWRLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDIIFGPQTLHRLPEMINSVRGDRS 123
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
V + K F+ L T AF+ I +GCN++C YC++PYTRG S+ +
Sbjct: 124 PVVDISFPEIEK-FDRLPEPRAEGPT-AFVSIMEGCNKYCTYCVVPYTRGEEVSRPSDDI 181
Query: 177 LDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKV-TLVDLLEEIEKIDGIERVRIGSIDP 234
L EI LA G +E+ L G + ++ G + D T DLL + IDGI+R+R + P
Sbjct: 182 LFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGTFADLLRLVAAIDGIDRIRFTTSHP 241
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
FT+D++ +L HL +QSG D L M R +TA EY+ I+ LR D+
Sbjct: 242 IEFTDDIIEVYRDTPELVSFLHLPVQSGSDRVLNLMGRTHTALEYKAIIRKLRAARPDIQ 301
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
I++D IVGFPG + + ++ F +S R GT A +M + V +K +R
Sbjct: 302 ISSDFIVGFPGETTDDFEKTMKLIADVNFDMSYSFIFSARPGTPAADMVDDVPEEEKKQR 361
Query: 355 SKKLIELNRINEKAFA--EKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVS 412
L E RIN++A A + +G +L E +G T N VN + +
Sbjct: 362 LYILQE--RINQQAMAWSRRMLGTTQRILVEGTSRKNIMELSGRTENNRVVNFEGTPEMI 419
Query: 413 GKILNVKI-----NSFEGEVAEGE 431
GK ++V+I NS G+V E
Sbjct: 420 GKFVDVEITDVYPNSLRGKVVRTE 443
>MIAB_SALCH RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 474
Score = 199 bits (506), Expect = 4e-53
Identities = 141/444 (31%), Positives = 214/444 (48%), Gaps = 18/444 (4%)
Query: 2 KVAFATLGCRVNTYESEAMTEKF-VREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S M + GY++ D E ADV ++NTCS+ +K +
Sbjct: 4 KLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVLLLNTCSIREKAQEKVFHQL 63
Query: 61 GRAR---RQNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR R +NP+ II V GC A + VD++ G + + +N R +
Sbjct: 64 GRWRLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDIIFGPQTLHRLPEMINSVRGDRS 123
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
V + K F+ L T AF+ I +GCN++C YC++PYTRG S+ +
Sbjct: 124 PVVDISFPEIEK-FDRLPEPRAEGPT-AFVSIMEGCNKYCTYCVVPYTRGEEVSRPSDDI 181
Query: 177 LDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKV-TLVDLLEEIEKIDGIERVRIGSIDP 234
L EI LA G +E+ L G + ++ G + D T DLL + IDGI+R+R + P
Sbjct: 182 LFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGTFADLLRLVAAIDGIDRIRFTTSHP 241
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
FT+D++ +L HL +QSG D L M R +TA EY+ I+ LR D+
Sbjct: 242 IEFTDDIIEVYRDTPELVSFLHLPVQSGSDRVLNLMGRTHTALEYKAIIRKLRAARPDIQ 301
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
I++D IVGFPG + + ++ F +S R GT A +M + V +K +R
Sbjct: 302 ISSDFIVGFPGETTDDFEKTMKLIADVNFDMSYSFIFSARPGTPAADMVDDVPEEEKKQR 361
Query: 355 SKKLIELNRINEKAFA--EKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVS 412
L E RIN++A A + +G +L E +G T N VN + +
Sbjct: 362 LYILQE--RINQQAMAWSRRMLGTTQRILVEGTSRKNIMELSGRTENNRVVNFEGTPEMI 419
Query: 413 GKILNVKI-----NSFEGEVAEGE 431
GK ++V+I NS G+V E
Sbjct: 420 GKFVDVEITDVYPNSLRGKVVRTE 443
>MIAB_SALA4 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 474
Score = 199 bits (506), Expect = 4e-53
Identities = 141/444 (31%), Positives = 214/444 (48%), Gaps = 18/444 (4%)
Query: 2 KVAFATLGCRVNTYESEAMTEKF-VREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S M + GY++ D E ADV ++NTCS+ +K +
Sbjct: 4 KLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVLLLNTCSIREKAQEKVFHQL 63
Query: 61 GRAR---RQNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR R +NP+ II V GC A + VD++ G + + +N R +
Sbjct: 64 GRWRLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDIIFGPQTLHRLPEMINSVRGDRS 123
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
V + K F+ L T AF+ I +GCN++C YC++PYTRG S+ +
Sbjct: 124 PVVDISFPEIEK-FDRLPEPRAEGPT-AFVSIMEGCNKYCTYCVVPYTRGEEVSRPSDDI 181
Query: 177 LDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKV-TLVDLLEEIEKIDGIERVRIGSIDP 234
L EI LA G +E+ L G + ++ G + D T DLL + IDGI+R+R + P
Sbjct: 182 LFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGTFADLLRLVAAIDGIDRIRFTTSHP 241
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
FT+D++ +L HL +QSG D L M R +TA EY+ I+ LR D+
Sbjct: 242 IEFTDDIIEVYRDTPELVSFLHLPVQSGSDRVLNLMGRTHTALEYKAIIRKLRAARPDIQ 301
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
I++D IVGFPG + + ++ F +S R GT A +M + V +K +R
Sbjct: 302 ISSDFIVGFPGETTDDFEKTMKLIADVNFDMSYSFIFSARPGTPAADMVDDVPEEEKKQR 361
Query: 355 SKKLIELNRINEKAFA--EKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVS 412
L E RIN++A A + +G +L E +G T N VN + +
Sbjct: 362 LYILQE--RINQQAMAWSRRMLGTTQRILVEGTSRKNIMELSGRTENNRVVNFEGTPEMI 419
Query: 413 GKILNVKI-----NSFEGEVAEGE 431
GK ++V+I NS G+V E
Sbjct: 420 GKFVDVEITDVYPNSLRGKVVRTE 443
>MIAB_NEIG2 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 442
Score = 199 bits (506), Expect = 4e-53
Identities = 142/441 (32%), Positives = 207/441 (46%), Gaps = 15/441 (3%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVRE--GYEVVDFNEMADVYVVNTCSVTNMGDKKSRQI 59
KV T GC++N Y+SE M E G E V + AD+ + NTCSV +K
Sbjct: 3 KVFIRTFGCQMNEYDSEKMLSVLAEEHGGIEQVTQADEADIILFNTCSVREKAQEKVFSD 62
Query: 60 IGRAR---RQNPEAIIAVVGCYA-QIAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEG 115
+GR R +NP II V GC A Q + VDVV G + + + G
Sbjct: 63 LGRVRPLKEKNPGLIIGVAGCVASQEGENIIKRAPYVDVVFGPQTLHRLPKMIVDKETSG 122
Query: 116 KQQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKK 175
QV + K F+ L AF+ I +GC+++C++C++PYTRG S+
Sbjct: 123 LSQVDISFPEIEK-FDHLPPARVEGGA-AFVSIMEGCSKYCSFCVVPYTRGEEFSRPLND 180
Query: 176 VLDEIRSLAEHGFKEIILSGIHTASYGVDLD--EKVTLVDLLEEIEKIDGIERVRIGSID 233
VL EI +LA+ G KEI L G + +Y ++D E LL + +I GIER+R +
Sbjct: 181 VLTEIANLAQQGVKEINLLGQNVNAYRGEMDDGEICDFATLLRIVHEIPGIERMRFTTSH 240
Query: 234 PTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDV 293
P FT+ ++ L KL H HL +QSG D L M R YTA EY+ I+ LR D+
Sbjct: 241 PREFTDSIIECYRDLPKLVSHLHLPIQSGSDRVLSAMKRGYTALEYKSIIRKLRAIRPDL 300
Query: 294 SITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDE 353
+++D IVGFPG +K IA + +F YSPR GT A N+ + +K
Sbjct: 301 CLSSDFIVGFPGETEREFEQTLKLVKDIAFDLSFVFIYSPRPGTPAANLPDDTPHEEKVR 360
Query: 354 RSKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSG 413
R + L E+ + IG V L E + T N VN ++
Sbjct: 361 RLEALNEVIEAETARINQTMIGTVQRCLVEGISKKDPDQLQARTANNRVVNFTGTPDMIN 420
Query: 414 KILNVKIN-----SFEGEVAE 429
++++++I S G++ E
Sbjct: 421 QMIDLEITEAYTFSLRGKIVE 441
>MIAB_HAEIN RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 474
Score = 199 bits (506), Expect = 4e-53
Identities = 142/444 (31%), Positives = 220/444 (49%), Gaps = 18/444 (4%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVR-EGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S M + + G E+ + E ADV ++NTCS+ +K +
Sbjct: 4 KLHIKTWGCQMNEYDSSKMADLLLSTHGLELTEAPEEADVLLLNTCSIREKAQEKVFHQL 63
Query: 61 GR---ARRQNPEAIIAVVGCYAQIAPTEVSG-IEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR ++ NP +I V GC A + VD++ G + + +N+ R GK
Sbjct: 64 GRWKELKKNNPNLVIGVGGCVASQEGEHIRHRAPYVDIIFGPQTLHRLPEMINQIRG-GK 122
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
V + + + F+ L T AF+ I +GCN++C +C++PYTRG S+ V
Sbjct: 123 SSVVDVSFPEIEKFDRLPEPRAEGPT-AFVSIMEGCNKYCTFCVVPYTRGEEVSRPVDDV 181
Query: 177 LDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKV-TLVDLLEEIEKIDGIERVRIGSIDP 234
L EI LAE G +E+ L G + +Y G D ++ + +LL + IDGI+R+R + P
Sbjct: 182 LFEIAQLAEQGVREVNLLGQNVNAYRGPTHDGQICSFAELLRLVASIDGIDRLRFTTSHP 241
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
FT+D++ +L HL +Q+G D L M R +TA EY+ I+ LR D+
Sbjct: 242 IEFTDDIIDVYRDTPELVSFLHLPVQAGSDRVLTMMKRAHTALEYKSIIRKLRAVRPDIQ 301
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
I++D IVGFPG + ++ + F YS R GT A +M + V ++K +R
Sbjct: 302 ISSDFIVGFPGETAEDFEQTMNLIAQVNFDMSFSFVYSARPGTPAADMPDDVTEDEKKQR 361
Query: 355 SKKLIELNRINEKA--FAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVS 412
L E RIN++A F+ + +G VL E + TG T VN + +
Sbjct: 362 LYVLQE--RINQQAAQFSRRMLGTEQRVLVEGPSKKDIMELTGRTETNRIVNFQGSPEMI 419
Query: 413 GKILNVKI-----NSFEGEVAEGE 431
GK ++VKI NS GEV E
Sbjct: 420 GKFVDVKITDVYTNSLRGEVVRTE 443
>MIAB_HAEIG RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 474
Score = 199 bits (506), Expect = 4e-53
Identities = 142/444 (31%), Positives = 220/444 (49%), Gaps = 18/444 (4%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVR-EGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S M + + G E+ + E ADV ++NTCS+ +K +
Sbjct: 4 KLHIKTWGCQMNEYDSSKMADLLLSTHGLELTEAPEEADVLLLNTCSIREKAQEKVFHQL 63
Query: 61 GR---ARRQNPEAIIAVVGCYAQIAPTEVSG-IEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR ++ NP +I V GC A + VD++ G + + +N+ R GK
Sbjct: 64 GRWKELKKNNPNLVIGVGGCVASQEGEHIRHRAPYVDIIFGPQTLHRLPEMINQIRG-GK 122
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
V + + + F+ L T AF+ I +GCN++C +C++PYTRG S+ V
Sbjct: 123 SSVVDVSFPEIEKFDRLPEPRAEGPT-AFVSIMEGCNKYCTFCVVPYTRGEEVSRPVDDV 181
Query: 177 LDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKV-TLVDLLEEIEKIDGIERVRIGSIDP 234
L EI LAE G +E+ L G + +Y G D ++ + +LL + IDGI+R+R + P
Sbjct: 182 LFEIAQLAEQGVREVNLLGQNVNAYRGPTHDGQICSFAELLRLVASIDGIDRLRFTTSHP 241
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
FT+D++ +L HL +Q+G D L M R +TA EY+ I+ LR D+
Sbjct: 242 IEFTDDIIDVYRDTPELVSFLHLPVQAGSDRVLTMMKRAHTALEYKSIIRKLRAVRPDIQ 301
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
I++D IVGFPG + ++ + F YS R GT A +M + V ++K +R
Sbjct: 302 ISSDFIVGFPGETAEDFEQTMNLIAQVNFDMSFSFVYSARPGTPAADMPDDVTEDEKKQR 361
Query: 355 SKKLIELNRINEKA--FAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVS 412
L E RIN++A F+ + +G VL E + TG T VN + +
Sbjct: 362 LYVLQE--RINQQAAQFSRRMLGTEQRVLVEGPSKKDIMELTGRTETNRIVNFQGSPEMI 419
Query: 413 GKILNVKI-----NSFEGEVAEGE 431
GK ++VKI NS GEV E
Sbjct: 420 GKFVDVKITDVYTNSLRGEVVRTE 443
>MIAB_CORJK RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 512
Score = 199 bits (506), Expect = 4e-53
Identities = 121/389 (31%), Positives = 196/389 (50%), Gaps = 12/389 (3%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIGRARR- 65
T GC++N ++SE ++ GY DV V NTC+V DK+ +G+ +
Sbjct: 23 TFGCQMNVHDSERLSGLLEDSGYVPASEGVTPDVVVFNTCAVRENADKRLYGTLGQMKAI 82
Query: 66 --QNPEAIIAVVGCYAQI-APTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVKVG 122
++P IAV GC AQ T V VDVV G+ N G + + ++ K +V++
Sbjct: 83 KDEHPGMQIAVGGCMAQKDKQTVVDKAPWVDVVFGTHNLGSLPTLLERSAHNHKAEVEIF 142
Query: 123 AVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDEIRS 182
L+ F + + + ++ + GCN C +C++P RG + P ++L E+++
Sbjct: 143 DALEE--FPSVLPAKRESAYAGWVSVSVGCNNTCTFCIVPSLRGKEQDRRPGEILAEVKA 200
Query: 183 LAEHGFKEIILSGIHTASYGVDLDEKVTLVD------LLEEIEKIDGIERVRIGSIDPTF 236
L E G E+ L G + +YGV+ ++ D LL +I+G+ER+R S P
Sbjct: 201 LVEQGVSEVTLLGQNVNAYGVNFADETLERDRTAFSKLLRACGEIEGLERLRFTSPHPAE 260
Query: 237 FTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSIT 296
FT+DV+ + +CP H+ LQSG D LK M R Y +K++ I++ +R++I +IT
Sbjct: 261 FTDDVIDAMAETPAVCPQLHMPLQSGSDKVLKDMKRSYRSKKFLAILDKVRERIPHAAIT 320
Query: 297 TDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSK 356
TD+IVGFPG +++ S F+YSPR GT A MENQV +R +
Sbjct: 321 TDIIVGFPGETEEDFQATLDVVEKARFSSAFTFQYSPRPGTPAAEMENQVPKAVVQDRYE 380
Query: 357 KLIELNRINEKAFAEKYIGEVIDVLFEEE 385
+L+ L + K IG ++L + +
Sbjct: 381 RLLALQERISEEENRKLIGTTQELLVQAD 409
>MIAB_CORGB RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 526
Score = 199 bits (506), Expect = 4e-53
Identities = 130/438 (29%), Positives = 211/438 (48%), Gaps = 23/438 (5%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIGRAR-- 64
T GC++N ++SE ++ GY + D+ V NTC+V D + +G R
Sbjct: 34 TYGCQMNVHDSERLSGLLEEAGYVAAPEDATPDLVVFNTCAVRENADMRLYGTLGNLRSV 93
Query: 65 -RQNPEAIIAVVGCYAQI-APTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVKVG 122
+NP IAV GC AQ T V VDVV G+ N G + + +A + +V++
Sbjct: 94 KEKNPGMQIAVGGCLAQKDKDTVVKKAPWVDVVFGTHNIGSLPTLLQRAEHNAQAEVEIV 153
Query: 123 AVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDEIRS 182
L+ F + + + ++ + GCN C +C++P RG + P +L E+++
Sbjct: 154 DSLEQ--FPSVLPAKRESAYAGWVSVSVGCNNTCTFCIVPSLRGKEQDRRPGDILAEVQA 211
Query: 183 LAEHGFKEIILSGIHTASYGVDL------DEKVTLVDLLEEIEKIDGIERVRIGSIDPTF 236
L + G E+ L G + +YGV+ ++ LL +I+G+ERVR S P
Sbjct: 212 LVDQGVTEVTLLGQNVNAYGVNFVDPELERDRSAFSKLLRACGEIEGLERVRFTSPHPAE 271
Query: 237 FTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSIT 296
FT DV+ + +CP H+ LQSG D LK M R Y +K++ I++ +R KI SIT
Sbjct: 272 FTSDVIDAMAETPNICPQLHMPLQSGSDKVLKEMRRSYRSKKFLSILDEVRAKIPHASIT 331
Query: 297 TDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSK 356
TD+IVGFPG +K+ + + F+YSPR GT A ENQ+ ER +
Sbjct: 332 TDIIVGFPGETEEDFQATLDVVKKARFTSAYTFQYSPRPGTPAAEYENQLPKEVVQERYE 391
Query: 357 KLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSGKIL 416
+L+ K IG +++L V+ G G T+ +++ +A G+++
Sbjct: 392 RLMXXXXXXXXXXXXKLIGTTVELL----VQAGGGRKNDATK---RMSGRAR---DGRLV 441
Query: 417 N-VKINSFEGEVAEGEIV 433
+ +GE+ G+ V
Sbjct: 442 HFAPEGDIDGEIRPGDFV 459
>MIAB_BORA1 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 475
Score = 199 bits (506), Expect = 4e-53
Identities = 137/443 (30%), Positives = 215/443 (48%), Gaps = 15/443 (3%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVRE-GYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S+ M + + G E+ D E ADV + NTCSV +K +
Sbjct: 28 KIYIRTFGCQMNEYDSDKMVDVLREDQGLEMTDNPEEADVILFNTCSVREKAQEKVFSDL 87
Query: 61 GRA---RRQNPEAIIAVVGCYA-QIAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR ++ NP +I V GC A Q V VDVV G + + + + RDEG
Sbjct: 88 GRVQHLKKLNPNLVIGVGGCVASQEGAAIVKRAPYVDVVFGPQTLHRLPELIRRRRDEGV 147
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
QV + K F+++ T AF+ I +GC+++C++C++PYTRG S+ + V
Sbjct: 148 SQVDISFPEIEK-FDNMPPSRVEGAT-AFVSIMEGCSKYCSFCVVPYTRGEEVSRPFEDV 205
Query: 177 LDEIRSLAEHGFKEIILSGIHTASYGVDLDEKVTLVD---LLEEIEKIDGIERVRIGSID 233
L E+ LA+ G +E+ L G + +Y ++ + D LLE + +I GIER+R +
Sbjct: 206 LTEVADLADQGVREVTLLGQNVNAYRGRIEGSDEIADFAMLLEYVHEIPGIERIRYTTSH 265
Query: 234 PTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDV 293
P T+ +V L KL HL +Q+G D L M R YTA E++ +V LR ++
Sbjct: 266 PKEMTQRMVEAYARLPKLVSFLHLPVQAGSDRVLAAMKRGYTALEFKSVVRKLRAARPNL 325
Query: 294 SITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDE 353
++++D IVGFPG + + + F YS R GT A ++++ + K +
Sbjct: 326 TLSSDFIVGFPGETEEDFQKTMKLIADVGFDTSFSFVYSRRPGTPAADLQDDTPQDVKLK 385
Query: 354 RSKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSG 413
R ++L L A A+ +G +L E G T N VN + + G
Sbjct: 386 RLQQLQALINEQAAAIAQSMVGTRQRLLVEGPSRRDPNELMGRTENNRIVNFEGPSRLIG 445
Query: 414 KILNVKI-----NSFEGEVAEGE 431
+++V I NS G VA E
Sbjct: 446 NMVDVIITHAFTNSLRGRVAGTE 468
>MIAB_RHOS4 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 436
Score = 199 bits (505), Expect = 5e-53
Identities = 133/434 (30%), Positives = 216/434 (49%), Gaps = 16/434 (3%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIG 61
K+ T GC++N Y+SE M E +GY + + E AD+ ++NTC + +K +G
Sbjct: 6 KLFIKTYGCQMNVYDSERMAEALGAKGYVLTEVAEEADMVLLNTCHIREKAAEKVYSDLG 65
Query: 62 RAR---RQNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGKQ 117
R R P+ I V GC AQ E+ + VD+V+G ++ + + R EG
Sbjct: 66 RLRPLKTAKPDLKIGVAGCVAQAEGEEILKRMPLVDLVVGPQSYHRLPDMLE--RTEGGA 123
Query: 118 QVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVL 177
+V + F+ L + AFL +Q+GC++FCA+C++PYTRG+ S+ +++
Sbjct: 124 RVVDTDFPEEDKFDHLPERKATRGPAAFLTVQEGCDKFCAFCVVPYTRGAEVSRPFARLM 183
Query: 178 DEIRSLAEHGFKEIILSGIHTASYGVDLDEKVTLVDLLEEIEKIDGIERVRIGSIDPTFF 237
E R L E G +EI L G + ++ D L L+ E+ +IDG+ER+R + P
Sbjct: 184 AEARGLVERGVREITLLGQNVNAWSND---GRGLGGLIRELARIDGLERLRYTTSHPNDM 240
Query: 238 TEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITT 297
+D++ A KL P+ HL +QSG D LK MNR++TA+ Y ++ +R D+ +T+
Sbjct: 241 ADDLIEAHGAEPKLMPYLHLPVQSGSDRILKAMNRKHTAEHYLRLIERIRAARPDILLTS 300
Query: 298 DVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSKK 357
D IVGFPG ++ + FKYS R GT A + ++ D R ++
Sbjct: 301 DFIVGFPGETEADFEATLDLIRAVGFGSAFSFKYSARPGTPAAE-KPELPAEVCDARLQR 359
Query: 358 LIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSGKILN 417
L L ++A +G + VL+E+ L G G + + V+ + G ++
Sbjct: 360 LQALVTEQQRAAQMAMVGREVGVLYEKAGRL-PGQMVGKSDHLHAVHVEDKAGQVGDLVR 418
Query: 418 VKI-----NSFEGE 426
V+I NS GE
Sbjct: 419 VRITASAPNSLAGE 432
>MIAB_ORITB RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 449
Score = 199 bits (505), Expect = 5e-53
Identities = 136/444 (30%), Positives = 227/444 (51%), Gaps = 25/444 (5%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIGRARRQ 66
T GC++N Y+S M + +GY +V A V ++NTC + +K +GR ++
Sbjct: 9 TYGCQMNVYDSNRMVDLLETQGYNIVANMADASVIILNTCHIREKASEKMYSELGRIKKL 68
Query: 67 NP--------EAIIAVVGCYAQIAPTEVSGIE-GVDVVLGSRNKGEIVYYVNKARDEGKQ 117
+A I V GC Q E+ E V++++G ++ + + K D G +
Sbjct: 69 QQSRLKAGKSKAKIIVAGCVGQAEGEEIFIREPAVNIIVGPQSYYNLPTMLEKL-DSGTE 127
Query: 118 QVKVG-----AVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKD 172
+ A NK+ E LK + + +Q+GC++FC +C++PYTRG+ S+
Sbjct: 128 NHLIDLDFVEAAKFNKLPEVLK----SPTVSGLVSVQEGCDKFCTFCVVPYTRGAEFSRP 183
Query: 173 PKKVLDEIRSLAEHGFKEIILSGIHTASY-GVDLDEK-VTLVDLLEEIEKIDGIERVRIG 230
++V E+ ++A+ G KE++L G + ++Y G D + K +L DL++ + KID I+R+R
Sbjct: 184 LEQVYREVLNIAQQGAKEVVLVGQNVSAYHGKDENGKECSLADLIKYVAKIDKIKRIRYI 243
Query: 231 SIDPTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKI 290
+ P T+ ++ +KL P HL +QSG + LK MNRR+T + Y +I+ LR+
Sbjct: 244 TSHPNDMTDQLLSLHATEEKLMPFLHLPVQSGSNKILKLMNRRHTRERYLEIIQQLRELR 303
Query: 291 EDVSITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNK 350
D+ I++D+IVGFPG K + + FKYS R GT A ++ Q+
Sbjct: 304 PDIVISSDIIVGFPGEDDEDFEATLSLAKEAKFGQCYSFKYSQRPGTPAA-VKQQISEEV 362
Query: 351 KDERSKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVY--TGYTRNYIKVNAKAD 408
K R L + + +K IG+V+ VLF+ + + + T Y ++ +N K D
Sbjct: 363 KQHRLSILQAQLMLQQLECNQKLIGKVVPVLFDRDGKYDGQIIGKTPYMQSVCIMNEK-D 421
Query: 409 CNVSGKILNVKINSFEGEVAEGEI 432
N+ GKI+NVKI S GE+
Sbjct: 422 NNLYGKIVNVKILSASASSLFGEV 445
>MIAB_ECO81 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 474
Score = 199 bits (505), Expect = 5e-53
Identities = 140/444 (31%), Positives = 215/444 (48%), Gaps = 18/444 (4%)
Query: 2 KVAFATLGCRVNTYESEAMTEKF-VREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S M + GY++ D E ADV ++NTCS+ +K +
Sbjct: 4 KLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVLLLNTCSIREKAQEKVFHQL 63
Query: 61 GR---ARRQNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR + +NP+ II V GC A + VD++ G + + +N R +
Sbjct: 64 GRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDIIFGPQTLHRLPEMINSVRGDRS 123
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
V + K F+ L T AF+ I +GCN++C YC++PYTRG S+ +
Sbjct: 124 PVVDISFPEIEK-FDRLPEPRAEGPT-AFVSIMEGCNKYCTYCVVPYTRGEEVSRPSDDI 181
Query: 177 LDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKV-TLVDLLEEIEKIDGIERVRIGSIDP 234
L EI LA G +E+ L G + ++ G + D T DLL + IDGI+R+R + P
Sbjct: 182 LFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGTFADLLRLVAAIDGIDRIRFTTSHP 241
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
FT+D++ +L HL +QSG D L M R +TA EY+ I+ LR D+
Sbjct: 242 IEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNLMGRTHTALEYKAIIRKLRAARPDIQ 301
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
I++D IVGFPG + + ++ F +S R GT A +M + V +K +R
Sbjct: 302 ISSDFIVGFPGETTEDFEKTMKLIADVNFDMSYSFIFSARPGTPAADMVDDVPEEEKKQR 361
Query: 355 SKKLIELNRINEKAFA--EKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVS 412
L E RIN++A A + +G +L E +G T N VN + ++
Sbjct: 362 LYILQE--RINQQAMAWSRRMLGTTQRILVEGTSRKSIMELSGRTENNRVVNFEGTPDMI 419
Query: 413 GKILNVKI-----NSFEGEVAEGE 431
GK ++V+I NS G+V E
Sbjct: 420 GKFVDVEITDVYPNSLRGKVVRTE 443
>MIAB_CLAMS RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 530
Score = 199 bits (505), Expect = 5e-53
Identities = 132/431 (30%), Positives = 209/431 (48%), Gaps = 17/431 (3%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIGR---A 63
T GC++N ++SE +T GY + E AD+ V+NTC+V D K +G
Sbjct: 25 TYGCQMNVHDSERLTGSLEAAGYVSAEGAE-ADIVVINTCAVRENADNKLYGNLGHLAGV 83
Query: 64 RRQNPEAIIAVVGCYAQI-APTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVKVG 122
+R++ IAV GC AQ T + VDVV G+ N G + + +AR G+ Q+++
Sbjct: 84 KRRHEGMQIAVGGCLAQKDRATVLEKAPWVDVVFGTHNMGALPTLLERARHNGEAQLEIL 143
Query: 123 AVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDEIRS 182
L+ F + + ++ I GCN C +C++P RG + P +L EI++
Sbjct: 144 ESLET--FPSTLPTKRDEIASGWVSISVGCNNTCTFCIVPALRGKEKDRRPGDILAEIQA 201
Query: 183 LAEHGFKEIILSGIHTASYGVDLDEKVTLVDLLEEIEKIDGIERVRIGSIDPTFFTEDVV 242
L + G E+ L G + SYGV+ ++ LL I+G+ER+R S P FT+DV+
Sbjct: 202 LVDDGAVEVTLLGQNVNSYGVEFGDRQAFGKLLRAAGAIEGLERIRFTSPHPAAFTDDVI 261
Query: 243 RRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITTDVIVG 302
+ + P H+ LQSG D LK M R Y ++ + I++ +R ++ D +ITTD+IVG
Sbjct: 262 DAMAETPAVMPQLHMPLQSGSDRILKAMRRSYRSERFLGILDRVRTRVPDAAITTDIIVG 321
Query: 303 FPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSKKLIELN 362
FPG ++ S F+YS R GT A ME QV + ER +L L
Sbjct: 322 FPGETEEDFQETLRVVEAARFSSAFTFQYSIRPGTPAATMEEQVPADVVKERYGRLTALQ 381
Query: 363 RINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSGKILNVKINS 422
++ +G ++VL V G G TR +V +A G+++++ +
Sbjct: 382 ERISHEENQRVVGRTVEVL----VSAHEGRKDGDTR---RVTGRAQ---DGRLVHLDVPE 431
Query: 423 FEGEVAEGEIV 433
E G+ V
Sbjct: 432 GSAEPRPGDAV 442
>MIAB_STAES RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 514
Score = 198 bits (504), Expect = 6e-53
Identities = 126/422 (29%), Positives = 210/422 (49%), Gaps = 10/422 (2%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVV-DFNEMADVYVVNTCSVTNMGDKKSRQIIGRAR- 64
T GC++N +++E M GY D NE ADV ++NTC++ + K IG +
Sbjct: 74 TYGCQMNAHDTEVMAGILNALGYSATSDINE-ADVILINTCAIRENAENKVFSEIGNLKH 132
Query: 65 --RQNPEAIIAVVGCYAQ---IAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQV 119
++ P+ +I V GC +Q + + + VD+V G+ N + + +A V
Sbjct: 133 LKKERPDCLIGVCGCMSQEESVVNKILKSYQNVDMVFGTHNIHHLPEILEEAYLSKAMVV 192
Query: 120 KVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDE 179
+V + + + E+L + + +A++ I GC++FC YC++P+TRG S+ P+ ++DE
Sbjct: 193 EVWSK-EGDIIENLP-KVRDGHIKAWVNIMYGCDKFCTYCIVPFTRGKERSRRPEDIIDE 250
Query: 180 IRSLAEHGFKEIILSGIHTASYGVDLDEKVTLVDLLEEIEKIDGIERVRIGSIDPTFFTE 239
+R LA G++EI L G + SYG I RVR + P FT+
Sbjct: 251 VRELAREGYQEITLLGQNVNSYGKXXXXXXXXXXXXXXXXSKIDIPRVRFTTSHPWDFTD 310
Query: 240 DVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITTDV 299
++ I + PH HL +QSG + LK M R+YT + Y D+V+ +++ I +V++TTD+
Sbjct: 311 RMIEVIAKGGNIVPHIHLPVQSGNNQVLKIMGRKYTRESYLDLVSRIKEAIPNVALTTDI 370
Query: 300 IVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSKKLI 359
IVG+P + + + YS R GT A M++ V K ER ++L
Sbjct: 371 IVGYPNETEEQFEETLSLYDDVQFEHAYTYLYSQRDGTPAAKMKDNVPLEVKKERLQRLN 430
Query: 360 ELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSGKILNVK 419
+ I + +Y G+++ VL E + V GYT VN K GK+++VK
Sbjct: 431 KKVGIYSQQAMSQYEGKIVTVLCEGSSKKDENVLAGYTDKNKLVNFKGPRESIGKLVDVK 490
Query: 420 IN 421
I+
Sbjct: 491 ID 492
>MIAB_STAEQ RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 514
Score = 198 bits (504), Expect = 6e-53
Identities = 126/422 (29%), Positives = 210/422 (49%), Gaps = 10/422 (2%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVV-DFNEMADVYVVNTCSVTNMGDKKSRQIIGRAR- 64
T GC++N +++E M GY D NE ADV ++NTC++ + K IG +
Sbjct: 74 TYGCQMNAHDTEVMAGILNALGYSATSDINE-ADVILINTCAIRENAENKVFSEIGNLKH 132
Query: 65 --RQNPEAIIAVVGCYAQ---IAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQV 119
++ P+ +I V GC +Q + + + VD+V G+ N + + +A V
Sbjct: 133 LKKERPDCLIGVCGCMSQEESVVNKILKSYQNVDMVFGTHNIHHLPEILEEAYLSKAMVV 192
Query: 120 KVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDE 179
+V + + + E+L + + +A++ I GC++FC YC++P+TRG S+ P+ ++DE
Sbjct: 193 EVWSK-EGDIIENLP-KVRDGHIKAWVNIMYGCDKFCTYCIVPFTRGKERSRRPEDIIDE 250
Query: 180 IRSLAEHGFKEIILSGIHTASYGVDLDEKVTLVDLLEEIEKIDGIERVRIGSIDPTFFTE 239
+R LA G++EI L G + SYG I RVR + P FT+
Sbjct: 251 VRELAREGYQEITLLGQNVNSYGKXXXXXXXXXXXXXXXXSKIDIPRVRFTTSHPWDFTD 310
Query: 240 DVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITTDV 299
++ I + PH HL +QSG + LK M R+YT + Y D+V+ +++ I +V++TTD+
Sbjct: 311 RMIEVIAKGGNIVPHIHLPVQSGNNQVLKIMGRKYTRESYLDLVSRIKEAIPNVALTTDI 370
Query: 300 IVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSKKLI 359
IVG+P + + + YS R GT A M++ V K ER ++L
Sbjct: 371 IVGYPNETEEQFEETLSLYDDVQFEHAYTYLYSQRDGTPAAKMKDNVPLEVKKERLQRLN 430
Query: 360 ELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSGKILNVK 419
+ I + +Y G+++ VL E + V GYT VN K GK+++VK
Sbjct: 431 KKVGIYSQQAMSQYEGKIVTVLCEGSSKKDENVLAGYTDKNKLVNFKGPRESIGKLVDVK 490
Query: 420 IN 421
I+
Sbjct: 491 ID 492
>MIAB_NITHX RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 473
Score = 198 bits (504), Expect = 6e-53
Identities = 129/436 (29%), Positives = 215/436 (49%), Gaps = 15/436 (3%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIG 61
K+ + GC++N Y+++ M + EG+ E AD+ ++NTC + +K +G
Sbjct: 6 KLHIKSYGCQMNVYDAQRMVDTLAAEGFVETASAEEADLVILNTCHIREKASEKVYSELG 65
Query: 62 RAR-------RQNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARD 113
R R R E + V GC AQ E+ VDVV+G ++ + + +A+
Sbjct: 66 RLRAAKDQAARDGREMSVVVAGCVAQAEGDEIIRRAPVVDVVVGPQSYHHLPQLLARAKA 125
Query: 114 EGKQQVKVGAVLKNKVFEDLKIEEYNNKTR---AFLKIQDGCNRFCAYCLIPYTRGSVCS 170
+G+ ++ +++K + + R AF+ +Q+GC++FC +C++PYTRG+ S
Sbjct: 126 DGRA-LETEFPVEDKFGFLPQPSRQAIRARGISAFVTVQEGCDKFCTFCVVPYTRGAEVS 184
Query: 171 KDPKKVLDEIRSLAEHGFKEIILSGIHTASYGVDLDE--KVTLVDLLEEIEKIDGIERVR 228
+ K++++++ LA++G +EI L G + +Y D + LL + I GI R+R
Sbjct: 185 RPVAKIVEDVQRLADNGVREITLIGQNVNAYHGDGPDGRPWPFGKLLHRLADIPGIVRLR 244
Query: 229 IGSIDPTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRD 288
+ P + ++ L L P HL +QSG D L+ MNRR+TA +Y +V+ R
Sbjct: 245 YSTSHPRDVEDSLIEAHRDLGALMPFVHLPVQSGSDRILEAMNRRHTADDYRRVVDRFRH 304
Query: 289 KIEDVSITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDG 348
+D++ ++D IVGFPG + +I + + FKYSPR GT A M+ V
Sbjct: 305 VRQDIAFSSDFIVGFPGETEQDFTATLALVTQIGYAGAYSFKYSPRPGTPAAEMKETVST 364
Query: 349 NKKDERSKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKAD 408
DER +L L + AF +G +DVLFE G G T + A
Sbjct: 365 ADMDERLVRLQNLIDSQQSAFNRAAVGTTVDVLFERAAR-NPGQIVGRTAYLQPAHVVAS 423
Query: 409 CNVSGKILNVKINSFE 424
++ G+IL V + S E
Sbjct: 424 ADIIGQILPVTVASLE 439
>MIAB_NEIMF RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 442
Score = 198 bits (504), Expect = 6e-53
Identities = 142/441 (32%), Positives = 207/441 (46%), Gaps = 15/441 (3%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVRE--GYEVVDFNEMADVYVVNTCSVTNMGDKKSRQI 59
KV T GC++N Y+S+ M E G E V + AD+ + NTCSV +K
Sbjct: 3 KVFIRTFGCQMNEYDSDKMLAVLAEEHGGIEQVTQADEADIILFNTCSVREKAQEKVFSD 62
Query: 60 IGRAR---RQNPEAIIAVVGCYA-QIAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEG 115
+GR R +NP II V GC A Q + VDVV G + + + G
Sbjct: 63 LGRVRPLKEKNPGLIIGVAGCVASQEGENIIKRAPYVDVVFGPQTLHRLPKMIVDKETSG 122
Query: 116 KQQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKK 175
QV + K F+ L AF+ I +GC+++C++C++PYTRG S+
Sbjct: 123 LSQVDISFPEIEK-FDHLPPARVEGGA-AFVSIMEGCSKYCSFCVVPYTRGEEFSRPLND 180
Query: 176 VLDEIRSLAEHGFKEIILSGIHTASYGVDLD--EKVTLVDLLEEIEKIDGIERVRIGSID 233
VL EI +LA+ G KEI L G + +Y ++D E LL + +I GIER+R +
Sbjct: 181 VLTEIANLAQQGVKEINLLGQNVNAYRGEMDDGEICDFATLLRIVHEIPGIERMRFTTSH 240
Query: 234 PTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDV 293
P FT+ ++ L KL H HL +QSG D L M R YTA EY+ I+ LR D+
Sbjct: 241 PREFTDSIIECYRDLPKLVSHLHLPIQSGSDRVLSAMKRGYTALEYKSIIRKLRAIRPDL 300
Query: 294 SITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDE 353
+++D IVGFPG +K IA + +F YSPR GT A N+ + +K
Sbjct: 301 CLSSDFIVGFPGETEREFEQTLKLVKDIAFDLSFVFIYSPRPGTPAANLHDDTPHEEKVR 360
Query: 354 RSKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSG 413
R + L E+ + IG V L E + T N VN ++
Sbjct: 361 RLEALNEVIEAETARINQTMIGTVQRCLVEGISKKDPDQLQARTANNRVVNFTGTPDMIN 420
Query: 414 KILNVKIN-----SFEGEVAE 429
++++++I S G+V E
Sbjct: 421 QMIDLEITEAYTFSLRGKVVE 441
>MIAB_NEIG1 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 442
Score = 198 bits (504), Expect = 6e-53
Identities = 142/441 (32%), Positives = 207/441 (46%), Gaps = 15/441 (3%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVRE--GYEVVDFNEMADVYVVNTCSVTNMGDKKSRQI 59
KV T GC++N Y+SE M E G E V + AD+ + NTCSV +K
Sbjct: 3 KVFIRTFGCQMNEYDSEKMLSVLAEEHGGIEQVTQADEADIILFNTCSVREKAQEKVFSD 62
Query: 60 IGRAR---RQNPEAIIAVVGCYA-QIAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEG 115
+GR R +NP II V GC A Q + VDVV G + + + G
Sbjct: 63 LGRVRPLKEKNPGLIIGVAGCVASQEGENIIKRAPYVDVVFGPQTLHRLPKMIVDKETSG 122
Query: 116 KQQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKK 175
QV + K F+ L AF+ I +GC+++C++C++PYTRG S+
Sbjct: 123 LSQVDISFPEIEK-FDHLPPARVEGGA-AFVSIMEGCSKYCSFCVVPYTRGEEFSRPLND 180
Query: 176 VLDEIRSLAEHGFKEIILSGIHTASYGVDLD--EKVTLVDLLEEIEKIDGIERVRIGSID 233
VL EI +LA+ G KEI L G + +Y ++D E LL + +I GIER+R +
Sbjct: 181 VLTEIANLAQQGVKEINLLGQNVNAYRGEMDDGEICDFATLLRIVHEIPGIERMRFTTSH 240
Query: 234 PTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDV 293
P FT+ ++ L KL H HL +QSG D L M R YTA EY+ I+ LR D+
Sbjct: 241 PREFTDSIIECYRDLPKLVSHLHLPIQSGSDRVLSAMKRGYTALEYKSIIRKLRAIRPDL 300
Query: 294 SITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDE 353
+++D IVGFPG +K IA + +F YSPR GT A N+ + +K
Sbjct: 301 CLSSDFIVGFPGETEREFEQTLKLVKDIAFDLSFVFIYSPRPGTPAANLPDDTPHEEKVR 360
Query: 354 RSKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSG 413
R + L E+ + IG V L E + T N VN ++
Sbjct: 361 RLEALNEVIEAETARINQTMIGTVQRCLVEGISKKDPDQLQARTVNNRVVNFTGTPDMIN 420
Query: 414 KILNVKIN-----SFEGEVAE 429
++++++I S G++ E
Sbjct: 421 QMIDLEITEAYTFSLRGKIVE 441
>MIAB_BARBK RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 458
Score = 198 bits (504), Expect = 6e-53
Identities = 138/444 (31%), Positives = 214/444 (48%), Gaps = 20/444 (4%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIG 61
KV T GC++N Y+S+ M + +GY AD+ +VNTC + +K +G
Sbjct: 16 KVFIKTYGCQMNVYDSQRMNDSLSAQGYVTTQTPNDADLILVNTCHIREKAAEKLYSDLG 75
Query: 62 RARRQNPEA------IIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDE 114
R R + +I V GC AQ E+ VD+V+G + + + +A+ +
Sbjct: 76 RLRMMRQKRTSEKPLMIGVTGCVAQAEGDEILRRSPTVDLVVGPQMYHRLPELLQQAQ-Q 134
Query: 115 GKQQVKVGAVLKNKVFEDL----KIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCS 170
GK+ V+ +++K F L K AFL +Q+GC++FC +C++PYTRG+ S
Sbjct: 135 GKKIVETNYAVEDK-FNHLPPHNKRAVQKRGVSAFLTVQEGCDKFCTFCVVPYTRGAEIS 193
Query: 171 KDPKKVLDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKV-TLVDLLEEIEKIDGIERVR 228
+ +++ DE R L E KEI L G + + G D K L DLL + K+DG++R+R
Sbjct: 194 RSVEQITDEARQLIEADVKEITLLGQNVNGWHGQSTDGKTWRLGDLLYHLAKLDGLKRLR 253
Query: 229 IGSIDPTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRD 288
+ P E ++ L L P+ HL +QSG D LK MNR++ A +Y +++ +R
Sbjct: 254 YTTSHPRDMDESLIAAHRNLDILMPYLHLPVQSGSDRILKAMNRQHKAIDYLNLIEKIRT 313
Query: 289 KIEDVSITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDG 348
D++ + D IVGFPG ++++ S + FKYSPR GT M+NQVD
Sbjct: 314 ARPDIAFSGDFIVGFPGETDDDFEETIKLIEQVGYSSAYSFKYSPRPGTLGATMKNQVDE 373
Query: 349 NKKDERSKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKV----N 404
K+ F IG+ DVL E++ G G + + V
Sbjct: 374 KVKNXXXXXXXXXXXXXXHRFLRSKIGQTTDVLIEKDGR-HPGQIVGRSPWLLPVVVDTQ 432
Query: 405 AKADCNVSGKILNVKINSFEGEVA 428
A ++ +I N NSF GE A
Sbjct: 433 APIGTVMAIQITNASSNSFVGEKA 456
>MIAB_ARTAT RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 515
Score = 198 bits (504), Expect = 6e-53
Identities = 128/380 (33%), Positives = 195/380 (51%), Gaps = 9/380 (2%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIGRARR- 65
T GC++N ++SE M GY D E+ADV V NTC+V D K +G+ R+
Sbjct: 31 TFGCQMNVHDSERMAGLLEEAGYVPAD-GEVADVVVFNTCAVRENADNKLYGNLGQLRQV 89
Query: 66 --QNPEAIIAVVGCYAQI-APTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVKVG 122
NP IAV GC AQ T V VD V G+ N G + +N+AR + Q+++
Sbjct: 90 KEANPGMQIAVGGCLAQKDRETIVKKAPWVDAVFGTHNVGALPALLNRARHNNEAQLEIL 149
Query: 123 AVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDEIRS 182
L VF + ++ ++ I GCN C +C++P RG + P ++L EI++
Sbjct: 150 ESLD--VFPSTLPTKRDSVYAGWVSISVGCNNTCTFCIVPSLRGKEKDRRPGEILAEIQA 207
Query: 183 LAEHGFKEIILSGIHTASYGVDLDEKVTLVDLLEEIEKIDGIERVRIGSIDPTFFTEDVV 242
L + G E+ L G + SYGV+ ++ LL +I+G+ERVR S P FT+DV+
Sbjct: 208 LVDDGAVEVTLLGQNVNSYGVEFGDRQAFSKLLRACGEIEGLERVRFTSPHPAAFTDDVI 267
Query: 243 RRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITTDVIVG 302
+ P H+ LQSG D LK M R Y + ++ I++ +RD+I +ITTD+IVG
Sbjct: 268 DAMAETHNAMPQLHMPLQSGSDKVLKDMRRSYRSSKFLGILDKVRDRIPHAAITTDIIVG 327
Query: 303 FPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSKKLIEL- 361
FPG +++ + F+YS R GT A ++ Q+ ER ++L L
Sbjct: 328 FPGETEEDFQATLDVVEKSRFASAFTFQYSKRPGTPAADLPEQLPKAVVQERYERLTALQ 387
Query: 362 NRINEKAFAEKYIGEVIDVL 381
+RI + A K +G ++VL
Sbjct: 388 DRIAAEENA-KQLGRKVEVL 406
>MIAB_PROM0 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 464
Score = 198 bits (503), Expect = 8e-53
Identities = 132/444 (29%), Positives = 215/444 (48%), Gaps = 20/444 (4%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIGRA--- 63
T GC++N +SE M + GY D AD+ + NTC++ + ++K +GR
Sbjct: 25 TFGCQMNKADSERMAGTLEKMGYTRADNELNADLVLYNTCTIRDNAEQKVYSFLGRQAKR 84
Query: 64 RRQNPEAIIAVVGCYAQI-APTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVKVG 122
+ + P + V GC AQ + + + +D+V+G ++ + + K D G Q V
Sbjct: 85 KHKTPRLKLVVAGCLAQQEGESLLRRVPELDLVMGPQHVNNLENLLGKV-DLGNQVV--- 140
Query: 123 AVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDEIRS 182
A + + ED+ + ++ I GCN C+YC++P RG S+ P + EI+
Sbjct: 141 ATEETFISEDITSARRESSICGWVNIIYGCNERCSYCVVPSVRGKEQSRYPNAIKSEIQK 200
Query: 183 LAEHGFKEIILSGIHTASYGVDLD-------EKVTLVDLLEEIEKIDGIERVRIGSIDPT 235
LA+ FKEI L G + +YG DL ++ TL DLL I + GI R+R + P
Sbjct: 201 LADDNFKEITLLGQNIDAYGRDLPGTTKEGRKENTLTDLLYYIHDVKGIRRIRFATSHPR 260
Query: 236 FFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSI 295
+F++ +++ L K+C HFH+ QSG D LK+M+R Y+ K+Y++I+ +R + D SI
Sbjct: 261 YFSKRLIQACYELDKVCEHFHIPFQSGNDEILKQMSRGYSIKKYKNIIENIRSLMPDASI 320
Query: 296 TTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERS 355
T D IV FPG + I + + YS R T A NQ+ K R
Sbjct: 321 TADAIVAFPGETEKQYQDTLKLISEIGFDQVNTAAYSARPNTPAAVWTNQLSEEVKKARL 380
Query: 356 KKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTR----NYIKVNAKADCNV 411
+++ +L ++ ++YI + VL E S G TR ++++ + N
Sbjct: 381 QEINDLVEKTARSRNQRYIDNIESVLIEGLNPKNSSQIMGRTRTNRLTFVEIPKNINFNF 440
Query: 412 S-GKILNVKINSFEGEVAEGEIVL 434
S G +NV+IN GE+ L
Sbjct: 441 SLGDEINVRINEARPFSLTGELSL 464
>MIAB_NOSP7 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 454
Score = 198 bits (503), Expect = 8e-53
Identities = 129/440 (29%), Positives = 212/440 (48%), Gaps = 35/440 (7%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIGR-ARR 65
T GC++N +SE M G+E + ADV + NTC++ + ++K +GR A+R
Sbjct: 12 TFGCQMNKADSERMAGVLEDMGFEWSEDPNNADVILYNTCTIRDNAEQKVYSYLGRQAKR 71
Query: 66 QN--PEAIIAVVGCYAQI-APTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVKVG 122
++ P+ + V GC AQ + + +D+V+G ++ + + D G Q V
Sbjct: 72 KHDQPDLTLIVAGCVAQQEGEALLRRVPELDLVMGPQHANRLKDLLESVFD-GNQVVATE 130
Query: 123 AVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDEIRS 182
+V + ED+ ++K A++ + GCN C YC++P RG S+ P + E+
Sbjct: 131 SV---HIIEDITQPRRDSKVTAWVNVIYGCNERCTYCVVPNVRGIEQSRTPSAIRAEMEE 187
Query: 183 LAEHGFKEIILSGIHTASYGVDLDEKV-------TLVDLLEEIEKIDGIERVRIGSIDPT 235
L G+KEI L G + +YG DL DLL + + GIER+R + P
Sbjct: 188 LGRQGYKEITLLGQNIDAYGRDLPGTTPEGRHLHNFTDLLYYVHDVPGIERLRFATSHPR 247
Query: 236 FFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSI 295
+FTE +++ L K+C HFH+ QSG + LK M R YT ++Y I++ +R + D SI
Sbjct: 248 YFTERLIKACAELPKVCKHFHIPFQSGDNELLKAMARGYTHEKYRRIIDTIRRYMPDASI 307
Query: 296 TTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERS 355
+ D IVGFPG ++ I + YSPR GT A +NQ+ K +R
Sbjct: 308 SADAIVGFPGETEAQFENTLKLVEDIGFDMLNTAAYSPRPGTPAALWDNQLSEEVKSDRL 367
Query: 356 KKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSGKI 415
++L L + +++Y G + +VL E++ N K V G+
Sbjct: 368 QRLNHLGNLKVAERSQRYFGRIEEVLVEDQ------------------NPKDQTQVMGRT 409
Query: 416 LNVKINSFEGEVAE--GEIV 433
++ F G++ E G++V
Sbjct: 410 DGNRLTFFSGDIKELKGQLV 429
>MIAB_MESSB RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 475
Score = 198 bits (503), Expect = 8e-53
Identities = 134/438 (30%), Positives = 217/438 (49%), Gaps = 17/438 (3%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIG 61
KV T GC++N Y+S+ M + EGY D E AD+ ++NTC + +K +G
Sbjct: 26 KVFVKTYGCQMNVYDSQRMADALAAEGYRATDVIEDADLVLLNTCHIREKAAEKVYSELG 85
Query: 62 RAR-------RQNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARD 113
R R +Q E ++ V GC AQ E+ VD+V+G + + V +AR
Sbjct: 86 RIRVLKEERAKQGRETVVGVAGCVAQAEGREILRRAPAVDLVIGPQTYHRLPSVVTRAR- 144
Query: 114 EGKQQVKVGAVLKNKVFEDLKIEEY----NNKTRAFLKIQDGCNRFCAYCLIPYTRGSVC 169
G++ V+ +++K F+ L E + AFL +Q+GC++FC +C++PYTRG+
Sbjct: 145 AGEKIVETEYAVEDK-FDHLPAPERTAVRSRGVTAFLTVQEGCDKFCTFCVVPYTRGAEV 203
Query: 170 SKDPKKVLDEIRSLAEHGFKEIILSGIHT-ASYGVDLDEKVT-LVDLLEEIEKIDGIERV 227
S+ +++ E LAE G +E+ L G + A +G D + L LL + +I G++R+
Sbjct: 204 SRPVAQIVAEAERLAEAGVRELTLLGQNVNAWHGEGPDGREWGLGRLLFRLAEIPGLDRL 263
Query: 228 RIGSIDPTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLR 287
R + P E+++ L KL P+ HL +Q+G D LK MNR++TA +Y ++ +R
Sbjct: 264 RYTTSHPRDMDEELIAAHRDLIKLMPYLHLPVQAGSDRILKAMNRKHTAADYLRLIERIR 323
Query: 288 DKIEDVSITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVD 347
D++++ D IVGFPG ++ + ++ FKYSPR GT +M +QV
Sbjct: 324 AARPDIAMSGDFIVGFPGETDRDFEDTMRIVRDVNYAQAFSFKYSPRPGTPGADMHDQVP 383
Query: 348 GNKKDERSKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKA 407
KD F E +G ID+L E+ +G G + V +
Sbjct: 384 DAVKDXXXXXXXXXXXXXXXXFGESLVGTEIDLLLEKPGR-QAGQLVGRSPWLQPVIVEE 442
Query: 408 DCNVSGKILNVKINSFEG 425
+ G I+ V+I S G
Sbjct: 443 NAGQIGDIVRVRITSSGG 460
>MIAB_HAEIE RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 474
Score = 198 bits (503), Expect = 8e-53
Identities = 142/444 (31%), Positives = 219/444 (49%), Gaps = 18/444 (4%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVR-EGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S M + + G E+ + E ADV ++NTCS+ +K +
Sbjct: 4 KLHIKTWGCQMNEYDSSKMADLLLSTHGLELTEAPEEADVLLLNTCSIREKAQEKVFHQL 63
Query: 61 GR---ARRQNPEAIIAVVGCYAQIAPTEVSG-IEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR ++ NP +I V GC A + VD++ G + + +N+ R GK
Sbjct: 64 GRWKELKKNNPNLVIGVGGCVASQEGEHIRHRAPYVDIIFGPQTLHRLPEMINQIRG-GK 122
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
V + + + F+ L T AF+ I +GCN++C +C++PYTRG S+ V
Sbjct: 123 SSVVDVSFPEIEKFDRLPEPRAEGPT-AFVSIMEGCNKYCTFCVVPYTRGEEVSRPVDDV 181
Query: 177 LDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKV-TLVDLLEEIEKIDGIERVRIGSIDP 234
L EI LAE G +E+ L G + +Y G D ++ + +LL + IDGI+R+R + P
Sbjct: 182 LFEIAQLAEQGVREVNLLGQNVNAYRGPTHDGQICSFAELLRLVASIDGIDRLRFTTSHP 241
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
FT D++ +L HL +Q+G D L M R +TA EY+ I+ LR D+
Sbjct: 242 IEFTNDIIDVYRDTPELVSFLHLPVQAGSDRVLTMMKRGHTALEYKSIIRKLRAVRPDIQ 301
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
I++D IVGFPG + ++ + F YS R GT A +M + V ++K +R
Sbjct: 302 ISSDFIVGFPGETAEDFEQTMNLIAQVNFDMSFSFVYSARPGTPAADMPDDVTEDEKKQR 361
Query: 355 SKKLIELNRINEKA--FAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVS 412
L E RIN++A F+ + +G VL E + TG T VN + +
Sbjct: 362 LYVLQE--RINQQAAQFSRRMLGTEQRVLVEGPSKKDIMELTGRTETNRIVNFQGSPEMI 419
Query: 413 GKILNVKI-----NSFEGEVAEGE 431
GK ++VKI NS GEV E
Sbjct: 420 GKFVDVKITDVYTNSLRGEVVRTE 443
>MIAB_HAEI8 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 474
Score = 198 bits (503), Expect = 8e-53
Identities = 142/444 (31%), Positives = 219/444 (49%), Gaps = 18/444 (4%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVR-EGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S M + + G E+ + E ADV ++NTCS+ +K +
Sbjct: 4 KLHIKTWGCQMNEYDSSKMADLLLSTHGLELTEAPEEADVLLLNTCSIREKAQEKVFHQL 63
Query: 61 GR---ARRQNPEAIIAVVGCYAQIAPTEVSG-IEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR ++ NP +I V GC A + VD++ G + + +N+ R GK
Sbjct: 64 GRWKELKKNNPNLVIGVGGCVASQEGEHIRHRAPYVDIIFGPQTLHRLPEMINQIRG-GK 122
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
V + + + F+ L T AF+ I +GCN++C +C++PYTRG S+ V
Sbjct: 123 SSVVDVSFPEIEKFDRLPEPRAEGPT-AFVSIMEGCNKYCTFCVVPYTRGEEVSRPVDDV 181
Query: 177 LDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKV-TLVDLLEEIEKIDGIERVRIGSIDP 234
L EI LAE G +E+ L G + +Y G D ++ + +LL + IDGI+R+R + P
Sbjct: 182 LFEIAQLAEQGVREVNLLGQNVNAYRGPTHDGQICSFAELLRLVASIDGIDRLRFTTSHP 241
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
FT D++ +L HL +Q+G D L M R +TA EY+ I+ LR D+
Sbjct: 242 IEFTNDIIDVYRDTPELVSFLHLPVQAGSDRVLTMMKRGHTALEYKSIIRKLRAVRPDIQ 301
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
I++D IVGFPG + ++ + F YS R GT A +M + V ++K +R
Sbjct: 302 ISSDFIVGFPGETAEDFEQTMNLIAQVNFDMSFSFVYSARPGTPAADMPDDVTEDEKKQR 361
Query: 355 SKKLIELNRINEKA--FAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVS 412
L E RIN++A F+ + +G VL E + TG T VN + +
Sbjct: 362 LYVLQE--RINQQAAQFSRRMLGTEQRVLVEGPSKKDIMELTGRTETNRIVNFQGSPEMI 419
Query: 413 GKILNVKI-----NSFEGEVAEGE 431
GK ++VKI NS GEV E
Sbjct: 420 GKFVDVKITDVYTNSLRGEVVRTE 443
>MIAB_BURP1 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 457
Score = 198 bits (503), Expect = 8e-53
Identities = 137/446 (30%), Positives = 213/446 (47%), Gaps = 15/446 (3%)
Query: 2 KVAFATLGCRVNTYESEAMTEKF-VREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
KV T GC++N Y+S+ M + EG E D E AD+ + NTCSV +K +
Sbjct: 4 KVYVKTFGCQMNEYDSDKMVDVLNAAEGLEKTDTPEDADIILFNTCSVREKAQEKVFSDL 63
Query: 61 GRARR---QNPEAIIAVVGCYA-QIAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR R P+ +I V GC A Q + V+ VD+V G + + ++ R+ G+
Sbjct: 64 GRVRELKEAKPDLLIGVGGCVASQEGASIVARAPYVDLVFGPQTLHRLPQMIDARRESGR 123
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
QV + K F+ L + AF+ I +GC+++C+YC++PYTRG S+ V
Sbjct: 124 AQVDITFPEIEK-FDHLPPARVEGPS-AFVSIMEGCSKYCSYCVVPYTRGDEVSRPLDDV 181
Query: 177 LDEIRSLAEHGFKEIILSGIHTASY----GVDLDEKVTLVDLLEEIEKIDGIERVRIGSI 232
L E+ LA+ G +E+ L G + +Y E L+E + I GIER+R +
Sbjct: 182 LTEVAGLADQGVREVTLLGQNVNAYRGAIAAGSAEIADFATLIEYVADIPGIERIRYTTS 241
Query: 233 DPTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIED 292
P FT+ ++ + KL H HL +Q G D L M R YT EY+ ++ LR +
Sbjct: 242 HPKEFTQRLLDVYAKVPKLVDHLHLPVQHGSDRILMAMKRGYTVLEYKSVIRKLRAIRPN 301
Query: 293 VSITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKD 352
+S++T++IVGFPG + ++ + F YSPR GT A N+ + K
Sbjct: 302 LSLSTNIIVGFPGETDADFDKTMALVHEMSYDTSFSFIYSPRPGTPAANLADDTPRELKL 361
Query: 353 ERSKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVN----AKAD 408
+R + L N ++ +G+V +L E G T N VN + A
Sbjct: 362 KRLQHLQATIEENVARISQSMLGKVERILVEGPSRKDPNELAGRTENNRVVNFPAPSAAH 421
Query: 409 CNVSGKILNVKINSFEGEVAEGEIVL 434
+ G++++VKIN GE+VL
Sbjct: 422 PRLIGQMIDVKINHAYPHSLRGELVL 447
>MIAB_BRUME RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 467
Score = 198 bits (503), Expect = 8e-53
Identities = 130/435 (29%), Positives = 220/435 (50%), Gaps = 17/435 (3%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIG 61
KV T GC++N Y+S+ M + EGY D + AD+ ++NTC + +K +G
Sbjct: 24 KVFVKTYGCQMNVYDSQRMADSLAAEGYVATDTPDDADLVLLNTCHIREKASEKLYSALG 83
Query: 62 RARRQNP-------EAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARD 113
R R+ E I V GC AQ E+ VD+V+G + + + + R
Sbjct: 84 RLRKMKDARAADGKELTIGVAGCVAQAEGQEILRRAPNVDLVIGPQTYHRLPNALARVRG 143
Query: 114 EGKQQVKVGAVLKNKVFEDL----KIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVC 169
G++ V+ +++K FE L + E AFL +Q+GC++FC +C++PYTRGS
Sbjct: 144 -GEKVVETDYAIEDK-FEHLPAPRREETRKRGVSAFLTVQEGCDKFCTFCVVPYTRGSEV 201
Query: 170 SKDPKKVLDEIRSLAEHGFKEIILSGIHTASYGVDLDE--KVTLVDLLEEIEKIDGIERV 227
S+ K+++ E LA+ G +E+ L G + ++ + ++ + L +LL + +I GI +
Sbjct: 202 SRSVKQIVAEAERLADSGVRELTLLGQNVNAWHGEGEDGREWGLGELLFRLARIPGIAHL 261
Query: 228 RIGSIDPTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLR 287
R + P + ++ L++L P+ HL +QSG D LK MNRR+ A EY ++ +R
Sbjct: 262 RYTTSHPRDMDDSLIAAHRDLRQLMPYLHLPVQSGSDRILKAMNRRHKADEYLRLIERIR 321
Query: 288 DKIEDVSITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVD 347
+ D++++ D IVGFPG ++ + ++ + FKYSPR GT ++++ V+
Sbjct: 322 NVRPDMALSGDFIVGFPGETDQDFEDTMQLVRDVNYAQAYSFKYSPRPGTPGADLDDHVE 381
Query: 348 GNKKDERSKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKA 407
KDE AF + IG +DVL E+ +G G + + V
Sbjct: 382 EAVKDEXXXXXXXXXXXXXYAFQDSMIGRKMDVLLEKPGR-EAGQMVGRSPWLLPVIIDD 440
Query: 408 DCNVSGKILNVKINS 422
+ + G I++VKI S
Sbjct: 441 NKDRVGDIIHVKIVS 455
>MIAB_BARHE RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 458
Score = 198 bits (503), Expect = 8e-53
Identities = 135/445 (30%), Positives = 216/445 (48%), Gaps = 24/445 (5%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIG 61
KV T GC++N Y+S+ MT+ +GY AD+ +VNTC + +K +G
Sbjct: 16 KVFIKTYGCQMNVYDSQRMTDSLSSKGYVATQTPNDADLILVNTCHIREKAAEKLYSDLG 75
Query: 62 RARRQNPEA------IIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDE 114
R R E ++ V GC AQ +E+ VD+V+G + + + + + +
Sbjct: 76 RLRVMRQERTPDKPLMVGVTGCVAQAEGSEILRRAPIVDLVIGPQMYHRLPDLLEQTK-Q 134
Query: 115 GKQQVKVGAVLKNKVFEDLKIEEYNNKT------RAFLKIQDGCNRFCAYCLIPYTRGSV 168
GK+ V +++K + +N + AFL +Q+GC++FC +C++PYTRG+
Sbjct: 135 GKKIVATEYAVEDKFAH---LPPHNKRAVRKRGVSAFLTVQEGCDKFCTFCVVPYTRGAE 191
Query: 169 CSKDPKKVLDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKV-TLVDLLEEIEKIDGIER 226
S+ +++ +E R L E G KEI L G + + G +++ K L DLL + K+DG++R
Sbjct: 192 ISRSVEQITEEARQLIEAGVKEITLLGQNVNGWHGQNVNGKTWRLGDLLYHLAKLDGLKR 251
Query: 227 VRIGSIDPTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLL 286
+R + P + ++ L L P+ HL +QSG D LK MNR++ + Y ++ +
Sbjct: 252 LRYTTSHPRDMDDSLIAAHRDLDILMPYLHLPVQSGSDRILKAMNRQHKSIHYLQLIEKI 311
Query: 287 RDKIEDVSITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQV 346
R+ D++ + D IVGFPG +K++ S + FKYSPR GT M+N V
Sbjct: 312 RNARPDIAFSGDFIVGFPGETDEDFEETIKLIKQVEYSSAYSFKYSPRPGTVGATMKNHV 371
Query: 347 DGNKKDERSKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKV--- 403
+ KD R AF IG+ DVL E+ SG G + + V
Sbjct: 372 EEAVKDARXXXXXXXXXXXXNAFLRSKIGQTTDVLI-EKAGRHSGQMVGRSPWLLPVVVD 430
Query: 404 -NAKADCNVSGKILNVKINSFEGEV 427
A + I N NSF GE+
Sbjct: 431 TQASTGTVIPIHIKNTSSNSFVGEM 455
>MIAB_SHISS RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 474
Score = 197 bits (502), Expect = 1e-52
Identities = 139/444 (31%), Positives = 215/444 (48%), Gaps = 18/444 (4%)
Query: 2 KVAFATLGCRVNTYESEAMTEKF-VREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S M + GY++ D E ADV ++NTCS+ +K +
Sbjct: 4 KLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVLLLNTCSIREKAQEKVFHQL 63
Query: 61 GR---ARRQNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR + +NP+ II V GC A + VD++ G + + +N R +
Sbjct: 64 GRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDIIFGPQTLHRLPEMINSVRGDRS 123
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
V + K F+ L T AF+ I +GCN++C YC++PYTRG S+ +
Sbjct: 124 PVVDISFPEIEK-FDRLPEPRAEGPT-AFVSIMEGCNKYCTYCVVPYTRGEEVSRPSDDI 181
Query: 177 LDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKV-TLVDLLEEIEKIDGIERVRIGSIDP 234
L EI LA G +E+ L G + ++ G + D + DLL + IDGI+R+R + P
Sbjct: 182 LFEIAQLAAQGVREVNLLGQNVNAWRGENYDGSTGSFADLLRLVAAIDGIDRIRFTTSHP 241
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
FT+D++ +L HL +QSG D L M R +TA EY+ I+ LR D+
Sbjct: 242 IEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNLMGRTHTALEYKAIIRKLRAARPDIQ 301
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
I++D IVGFPG + + ++ F +S R GT A +M + V +K +R
Sbjct: 302 ISSDFIVGFPGETTEDFEKTMKLIADVNFDMSYSFIFSARPGTPAADMVDDVPEEEKKQR 361
Query: 355 SKKLIELNRINEKAFA--EKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVS 412
L E RIN++A A + +G +L E +G T N VN + ++
Sbjct: 362 LYILQE--RINQQAMAWSRRMLGTTQRILVEGTSRKSIMELSGRTENNRVVNFEGTPDMI 419
Query: 413 GKILNVKI-----NSFEGEVAEGE 431
GK ++V+I NS G+V E
Sbjct: 420 GKFVDVEITDVYPNSLRGKVVRTE 443
>MIAB_SHIBS RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 474
Score = 197 bits (502), Expect = 1e-52
Identities = 139/444 (31%), Positives = 215/444 (48%), Gaps = 18/444 (4%)
Query: 2 KVAFATLGCRVNTYESEAMTEKF-VREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S M + GY++ D E ADV ++NTCS+ +K +
Sbjct: 4 KLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVLLLNTCSIREKAQEKVFHQL 63
Query: 61 GR---ARRQNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR + +NP+ II V GC A + VD++ G + + +N R +
Sbjct: 64 GRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDIIFGPQTLHRLPEMINSVRGDRS 123
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
V + K F+ L T AF+ I +GCN++C YC++PYTRG S+ +
Sbjct: 124 PVVDISFPEIEK-FDRLPEPRAEGPT-AFVSIMEGCNKYCTYCVVPYTRGEEVSRPSDDI 181
Query: 177 LDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKV-TLVDLLEEIEKIDGIERVRIGSIDP 234
L EI LA G +E+ L G + ++ G + D + DLL + IDGI+R+R + P
Sbjct: 182 LFEIAQLAAQGVREVNLLGQNVNAWRGENYDGSTGSFADLLRLVAAIDGIDRIRFTTSHP 241
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
FT+D++ +L HL +QSG D L M R +TA EY+ I+ LR D+
Sbjct: 242 IEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNLMGRTHTALEYKAIIRKLRAARPDIQ 301
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
I++D IVGFPG + + ++ F +S R GT A +M + V +K +R
Sbjct: 302 ISSDFIVGFPGETTEDFEKTMKLIADVNFDMSYSFIFSARPGTPAADMVDDVPEEEKKQR 361
Query: 355 SKKLIELNRINEKAFA--EKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVS 412
L E RIN++A A + +G +L E +G T N VN + ++
Sbjct: 362 LYILQE--RINQQAMAWSRRMLGTTQRILVEGTSRKSIMELSGRTENNRVVNFEGTPDMI 419
Query: 413 GKILNVKI-----NSFEGEVAEGE 431
GK ++V+I NS G+V E
Sbjct: 420 GKFVDVEITDVYPNSLRGKVVRTE 443
>MIAB_RICTY RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 445
Score = 197 bits (502), Expect = 1e-52
Identities = 135/445 (30%), Positives = 231/445 (51%), Gaps = 23/445 (5%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIG 61
K+ T GC++N Y+S + + GYE + + AD+ ++NTC + +K+ +G
Sbjct: 4 KLYIKTYGCQMNVYDSVKIQDLLYPFGYESTEDIKEADIIILNTCHIREKAAEKTYSELG 63
Query: 62 RARR-QNPE-------AIIAVVGCYAQIAPTEV--SGIEGVDVVLGSRNKGEIVYYVNKA 111
R ++ QN AII V GC AQ A EV + VD+V+G ++ + ++K
Sbjct: 64 RIKKLQNTRKQEGLNPAIIVVAGCVAQ-AEGEVIFTRTPYVDIVVGPQSYYNLPELISKV 122
Query: 112 RDEGKQQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSK 171
K + + V + K F++L + Y +F+ +Q+GC++FC +C++PYTRG+ S+
Sbjct: 123 VRHEKHLIDLDFVEEAK-FDNLPEQLYTQGASSFISVQEGCDKFCTFCVVPYTRGAEFSR 181
Query: 172 DPKKVLDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKV-TLVDLLEEIEKIDGIERVRI 229
++V E + + KEIIL G + +Y G +K+ TL DLL+ + +I +ER+R
Sbjct: 182 SVEQVYRESLKVVSNDTKEIILLGQNVNAYHGKGPKDKIFTLADLLKHLAQIPNLERLRY 241
Query: 230 GSIDPTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDK 289
+ P T+D+++ KL P HL +QSG + LK MNR++ Y +I+N LR+
Sbjct: 242 MTSHPIDMTDDLIKLHGTEPKLMPFLHLPVQSGSNKILKAMNRKHDRDYYFNIINRLREA 301
Query: 290 IEDVSITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGN 349
D+ +++D IVGFPG ++R+ + + FKYSPR GT +Q+ +
Sbjct: 302 RSDIVLSSDFIVGFPGETEKDFEDTLDLVQRVKYGQCYSFKYSPRPGTPGAIRTDQIPEH 361
Query: 350 KKDERSKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVY--TGYTRNYIKVNAKA 407
K +R L + + AF E +G + VLF+ + + + T Y ++ N
Sbjct: 362 IKSKRLTILQKELATQQLAFNESCVGSTMKVLFDRDGKFEDQIIGKTPYMQSVYIHNPNK 421
Query: 408 DCNVSGKILNV-----KINSFEGEV 427
++ GKI++V +NS GE+
Sbjct: 422 --SLLGKIVDVIITKAALNSLTGEI 444
>MIAB_NEIMB RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 442
Score = 197 bits (502), Expect = 1e-52
Identities = 141/441 (31%), Positives = 207/441 (46%), Gaps = 15/441 (3%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVRE--GYEVVDFNEMADVYVVNTCSVTNMGDKKSRQI 59
KV T GC++N Y+S+ M E G E V + AD+ + NTCSV +K
Sbjct: 3 KVFIRTFGCQMNEYDSDKMLAVLAEEHGGIEQVTQADEADIILFNTCSVREKAQEKVFSD 62
Query: 60 IGRAR---RQNPEAIIAVVGCYA-QIAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEG 115
+GR R +NP II V GC A Q + VDVV G + + + G
Sbjct: 63 LGRVRPLKEKNPGLIIGVAGCVASQEGENIIKRAPYVDVVFGPQTLHRLPKMIVDKETSG 122
Query: 116 KQQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKK 175
QV + K F+ L AF+ I +GC+++C++C++PYTRG S+
Sbjct: 123 LSQVDISFPEIEK-FDHLPPARVEGGA-AFVSIMEGCSKYCSFCVVPYTRGEEFSRPLND 180
Query: 176 VLDEIRSLAEHGFKEIILSGIHTASYGVDLD--EKVTLVDLLEEIEKIDGIERVRIGSID 233
VL EI +LA+ G KEI L G + +Y ++D E LL + +I GIER+R +
Sbjct: 181 VLTEIANLAQQGVKEINLLGQNVNAYRGEMDDGEICDFATLLRIVHEIPGIERMRFTTSH 240
Query: 234 PTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDV 293
P FT+ ++ L KL H HL +QSG D L M R YTA EY+ I+ LR D+
Sbjct: 241 PREFTDSIIECYRDLPKLVSHLHLPIQSGSDRVLSAMKRGYTALEYKSIIRKLRAIRPDL 300
Query: 294 SITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDE 353
+++D IVGFPG +K IA + +F YSPR GT A N+ + +K
Sbjct: 301 CLSSDFIVGFPGETEREFEQTLKLVKDIAFDLSFVFIYSPRPGTPAANLPDDTPHEEKVR 360
Query: 354 RSKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSG 413
R + L E+ + +G V L E + T N VN ++
Sbjct: 361 RLEALNEVIEAETARINQTMVGTVQRCLVEGISKKDPDQLQARTANNRVVNFTGTPDMIN 420
Query: 414 KILNVKIN-----SFEGEVAE 429
++++++I S G+V E
Sbjct: 421 QMIDLEITEAYTFSLRGKVVE 441
>MIAB_HAEDU RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 475
Score = 197 bits (502), Expect = 1e-52
Identities = 141/444 (31%), Positives = 217/444 (48%), Gaps = 18/444 (4%)
Query: 2 KVAFATLGCRVNTYESEAMTEKF-VREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S M + G E+ D E AD+ ++NTCS+ +K +
Sbjct: 3 KLHITTWGCQMNEYDSSKMADLLNSTHGLELTDKPEEADILLLNTCSIREKAQEKVFSQL 62
Query: 61 GRAR---RQNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR + + P+ II V GC A + VD+V G + + +N+ R +
Sbjct: 63 GRWKNWKKDKPDLIIGVGGCVASQEGEHIRERAPFVDIVFGPQTLHRLPEMINQIRSGDR 122
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
V + K F+ L + T AF+ I +GCN++C++C++PYTRG S+ V
Sbjct: 123 AVVDISFPEIEK-FDRLPEPKAEGPT-AFVSIMEGCNKYCSFCVVPYTRGEEVSRPVDDV 180
Query: 177 LDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKV-TLVDLLEEIEKIDGIERVRIGSIDP 234
L EI LAE G +E+ L G + +Y G D + T +LL + IDGI+R+R + P
Sbjct: 181 LFEIAQLAEQGVREVNLLGQNVNAYRGETFDGGICTFAELLRLVAAIDGIDRLRYTTSHP 240
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
FT+D++ +L HL +QSG D L M R +TA EY+ I+ LR ++
Sbjct: 241 IEFTDDIIEVYRDTPELVSFLHLPIQSGADRVLTMMKRNHTALEYKAIIRKLRAVRPNIQ 300
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
I++D IVGFPG ++++ + F YS R GT A ++ + V N+K +R
Sbjct: 301 ISSDFIVGFPGETAEDFEQTMKIIEQVNFDMSFSFIYSARPGTPASDLPDDVSENEKKDR 360
Query: 355 SKKLIELNRINEKA--FAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVS 412
L + RIN +A F+ +G +L E + TG T VN N+
Sbjct: 361 LAHLQQ--RINHQAMQFSRLMLGTEQRILVEGPSKKDIMELTGRTETNRVVNFIGTPNMI 418
Query: 413 GKILNVKI-----NSFEGEVAEGE 431
GK +++KI NS GEV E
Sbjct: 419 GKFVDIKITDVYSNSLRGEVIRTE 442
>MIAB_CITK8 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 474
Score = 197 bits (502), Expect = 1e-52
Identities = 139/444 (31%), Positives = 215/444 (48%), Gaps = 18/444 (4%)
Query: 2 KVAFATLGCRVNTYESEAMTEKF-VREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S M + GY++ + E ADV ++NTCS+ +K +
Sbjct: 4 KLHIKTWGCQMNEYDSSKMADLLDATHGYQLTEVAEEADVLLLNTCSIREKAQEKVFHQL 63
Query: 61 GR---ARRQNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR + +NP+ II V GC A + VD++ G + + +N R +
Sbjct: 64 GRWKLLKEKNPDLIIGVGGCVASQEGDHIRQRAHYVDIIFGPQTLHRLPEMINSVRGDRS 123
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
V + K F+ L T AF+ I +GCN++C YC++PYTRG S+ +
Sbjct: 124 PVVDISFPEIEK-FDRLPEPRAEGPT-AFVSIMEGCNKYCTYCVVPYTRGEEVSRPSDDI 181
Query: 177 LDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKV-TLVDLLEEIEKIDGIERVRIGSIDP 234
L EI LA G +E+ L G + ++ G + D + DLL + IDGI+R+R + P
Sbjct: 182 LFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSFADLLRLVAAIDGIDRIRFTTSHP 241
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
FT+D++ +L HL +QSG D L M R +TA EY+ I+ LR D+
Sbjct: 242 IEFTDDIIEVYRDTPELVSFLHLPVQSGADRVLNLMGRTHTALEYKAIIRKLRAARPDIQ 301
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
I++D IVGFPG + + ++ F +S R GT A +M + V +K +R
Sbjct: 302 ISSDFIVGFPGETTQDFEQTMKLIADVNFDMSYSFIFSARPGTPAADMVDDVPEEEKKQR 361
Query: 355 SKKLIELNRINEKAFA--EKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVS 412
L E RIN++A A + +G V +L E +G T N VN + +
Sbjct: 362 LYILQE--RINQQAMAWSRRMLGTVQRILVEGTSRKSIMELSGRTENNRVVNFEGTPEMI 419
Query: 413 GKILNVKI-----NSFEGEVAEGE 431
GK ++V+I NS G+V E
Sbjct: 420 GKFVDVEITDVYTNSLRGKVVRTE 443
>MIAB_SHIFL RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 474
Score = 197 bits (501), Expect = 1e-52
Identities = 139/444 (31%), Positives = 215/444 (48%), Gaps = 18/444 (4%)
Query: 2 KVAFATLGCRVNTYESEAMTEKF-VREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S M + GY++ D E ADV ++NTCS+ +K +
Sbjct: 4 KLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVLLLNTCSIREKAQEKVFHQL 63
Query: 61 GR---ARRQNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR + +NP+ II V GC A + VD++ G + + +N R +
Sbjct: 64 GRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDIIFGPQTLHRLPEMINSVRGDRS 123
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
V + K F+ L T AF+ I +GCN++C YC++PYTRG S+ +
Sbjct: 124 PVVDISFPEIEK-FDRLPEPRAEGPT-AFVSIMEGCNKYCTYCVVPYTRGEEVSRPSDDI 181
Query: 177 LDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKV-TLVDLLEEIEKIDGIERVRIGSIDP 234
L EI LA G +E+ L G + ++ G + D + DLL + IDGI+R+R + P
Sbjct: 182 LFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSFADLLRLVAAIDGIDRIRFTTSHP 241
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
FT+D++ +L HL +QSG D L M R +TA EY+ I+ LR D+
Sbjct: 242 IEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNLMGRTHTALEYKAIIRKLRAARPDIQ 301
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
I++D IVGFPG + + ++ F +S R GT A +M + V +K +R
Sbjct: 302 ISSDFIVGFPGETTEDFEKTMKLIADVNFDMSYSFIFSARPGTPAADMVDDVPEEEKKQR 361
Query: 355 SKKLIELNRINEKAFA--EKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVS 412
L E RIN++A A + +G +L E +G T N VN + ++
Sbjct: 362 LYILQE--RINQQAMAWSRRMLGTTQRILVEGTSRKSIMELSGRTENNRVVNFEGTPDMI 419
Query: 413 GKILNVKI-----NSFEGEVAEGE 431
GK ++V+I NS G+V E
Sbjct: 420 GKFVDVEITDVYPNSLRGKVVRTE 443
>MIAB_SHIF8 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 474
Score = 197 bits (501), Expect = 1e-52
Identities = 139/444 (31%), Positives = 215/444 (48%), Gaps = 18/444 (4%)
Query: 2 KVAFATLGCRVNTYESEAMTEKF-VREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S M + GY++ D E ADV ++NTCS+ +K +
Sbjct: 4 KLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVLLLNTCSIREKAQEKVFHQL 63
Query: 61 GR---ARRQNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR + +NP+ II V GC A + VD++ G + + +N R +
Sbjct: 64 GRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDIIFGPQTLHRLPEMINSVRGDRS 123
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
V + K F+ L T AF+ I +GCN++C YC++PYTRG S+ +
Sbjct: 124 PVVDISFPEIEK-FDRLPEPRAEGPT-AFVSIMEGCNKYCTYCVVPYTRGEEVSRPSDDI 181
Query: 177 LDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKV-TLVDLLEEIEKIDGIERVRIGSIDP 234
L EI LA G +E+ L G + ++ G + D + DLL + IDGI+R+R + P
Sbjct: 182 LFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSFADLLRLVAAIDGIDRIRFTTSHP 241
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
FT+D++ +L HL +QSG D L M R +TA EY+ I+ LR D+
Sbjct: 242 IEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNLMGRTHTALEYKAIIRKLRAARPDIQ 301
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
I++D IVGFPG + + ++ F +S R GT A +M + V +K +R
Sbjct: 302 ISSDFIVGFPGETTEDFEKTMKLIADVNFDMSYSFIFSARPGTPAADMVDDVPEEEKKQR 361
Query: 355 SKKLIELNRINEKAFA--EKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVS 412
L E RIN++A A + +G +L E +G T N VN + ++
Sbjct: 362 LYILQE--RINQQAMAWSRRMLGTTQRILVEGTSRKSIMELSGRTENNRVVNFEGTPDMI 419
Query: 413 GKILNVKI-----NSFEGEVAEGE 431
GK ++V+I NS G+V E
Sbjct: 420 GKFVDVEITDVYPNSLRGKVVRTE 443
>MIAB_SHIDS RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 474
Score = 197 bits (501), Expect = 1e-52
Identities = 139/444 (31%), Positives = 215/444 (48%), Gaps = 18/444 (4%)
Query: 2 KVAFATLGCRVNTYESEAMTEKF-VREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S M + GY++ D E ADV ++NTCS+ +K +
Sbjct: 4 KLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVLLLNTCSIREKAQEKVFHQL 63
Query: 61 GR---ARRQNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR + +NP+ II V GC A + VD++ G + + +N R +
Sbjct: 64 GRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDIIFGPQTLHRLPEMINSVRGDRS 123
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
V + K F+ L T AF+ I +GCN++C YC++PYTRG S+ +
Sbjct: 124 PVVDISFPEIEK-FDRLPEPRAEGPT-AFVSIMEGCNKYCTYCVVPYTRGEEVSRPSDDI 181
Query: 177 LDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKV-TLVDLLEEIEKIDGIERVRIGSIDP 234
L EI LA G +E+ L G + ++ G + D + DLL + IDGI+R+R + P
Sbjct: 182 LFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSFADLLRLVAAIDGIDRIRFTTSHP 241
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
FT+D++ +L HL +QSG D L M R +TA EY+ I+ LR D+
Sbjct: 242 IEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNLMGRTHTALEYKAIIRKLRAARPDIQ 301
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
I++D IVGFPG + + ++ F +S R GT A +M + V +K +R
Sbjct: 302 ISSDFIVGFPGETTEDFEKTMKLIADVNFDMSYSFIFSARPGTPAADMVDDVPEEEKKQR 361
Query: 355 SKKLIELNRINEKAFA--EKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVS 412
L E RIN++A A + +G +L E +G T N VN + ++
Sbjct: 362 LYILQE--RINQQAMAWSRRMLGTTQRILVEGTSRKSIMELSGRTENNRVVNFEGTPDMI 419
Query: 413 GKILNVKI-----NSFEGEVAEGE 431
GK ++V+I NS G+V E
Sbjct: 420 GKFVDVEITDVYPNSLRGKVVRTE 443
>MIAB_RHOPA RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 463
Score = 197 bits (501), Expect = 1e-52
Identities = 131/448 (29%), Positives = 219/448 (48%), Gaps = 23/448 (5%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIG 61
K+ + GC++N Y+++ M + EG+ + AD+ ++NTC + +K +G
Sbjct: 6 KLHIKSYGCQMNVYDAQRMVDALAPEGFVETANVDDADLVILNTCHIREKASEKVYSELG 65
Query: 62 RARRQNPEAI-------IAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARD 113
R R EA IAV GC AQ E+ VDVV+G ++ + + KA
Sbjct: 66 RLRVARDEAANHGRRMQIAVAGCVAQAEGAEIIRRAPVVDVVVGPQSYHNLPQLLAKAEQ 125
Query: 114 EGKQ-------QVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRG 166
G+ + K G VL + ++ + AF+ +Q+GC++FC +C++PYTRG
Sbjct: 126 HGRALETEFPIEDKFG-VLPQPAPDAIRARGIS----AFVTVQEGCDKFCTFCVVPYTRG 180
Query: 167 SVCSKDPKKVLDEIRSLAEHGFKEIILSGIHTASYGVDLDEKV--TLVDLLEEIEKIDGI 224
+ S+ ++++++ LAE+G +E+ L G + +Y D +++ +L L+ + +I GI
Sbjct: 181 AEMSRPVAAIVEDVKRLAENGVREVTLIGQNVNAYHGDGPDRLAWSLGRLVRRLAEIPGI 240
Query: 225 ERVRIGSIDPTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVN 284
R+R + P +D++ L L P HL +QSG D L MNR++TA +Y +++
Sbjct: 241 VRLRYSTSHPNDVNDDLLAAHRDLPALMPFVHLPVQSGSDRILAAMNRKHTADDYRRVID 300
Query: 285 LLRDKIEDVSITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMEN 344
R E ++ ++D IVGFPG + +I + + FKYSPR GT A +M
Sbjct: 301 RFRLASEAIAFSSDFIVGFPGETERDFSATLALVAQIGYAGAYSFKYSPRPGTPAADMAE 360
Query: 345 QVDGNKKDERSKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVN 404
V DER AF + IG ++VLF E G G T +
Sbjct: 361 MVPAAVMDERXXXXXXXXXXXXSAFNKAAIGRTVEVLF-ERAGRKPGQIVGRTAYLQPAH 419
Query: 405 AKADCNVSGKILNVKINSFEGEVAEGEI 432
A ++ GK+L V+++S E GE+
Sbjct: 420 VMAPDSIIGKVLPVRVDSLERYSLLGEL 447
>MIAB_NITMU RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 474
Score = 197 bits (501), Expect = 1e-52
Identities = 142/442 (32%), Positives = 208/442 (47%), Gaps = 12/442 (2%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFV-REGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S+ M + + E D ADV + NTCSV +K +
Sbjct: 4 KLYIKTFGCQMNEYDSKKMADVLRDAQHMEKTDDPAAADVILFNTCSVREKAQEKVFHDL 63
Query: 61 GRARR---QNPEAIIAVVGCYAQIAPTE-VSGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR R NP+ +I V GC A E V VD+V G + + ++ + G+
Sbjct: 64 GRVRHLKAANPDLLIGVGGCVASQEGAEIVKRAPYVDLVFGPQTLHRLPQMISTRQITGR 123
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
QV + K F+ L T AF+ I +GC+++C++C++PYTRG S+ +
Sbjct: 124 PQVDISFPEIEK-FDHLPPARTEGVT-AFVSIMEGCSKYCSFCVVPYTRGEEISRPLDDI 181
Query: 177 LDEIRSLAEHGFKEIILSGIHTASYG--VDLDEKVTLVD---LLEEIEKIDGIERVRIGS 231
L EI L G KE+ L G + +Y + E+ L D LLE + +I GIER+R +
Sbjct: 182 LTEIAGLTNLGVKEVTLLGQNVNAYRGRMQYAEEGELADFALLLEYLHEIPGIERIRYTT 241
Query: 232 IDPTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIE 291
P FT ++ A KL H HL +QSG D L M R YT+ EY+ I+ LR
Sbjct: 242 SHPREFTPRLIEAYKASPKLVSHVHLPVQSGSDRILAAMKRGYTSLEYKSIIRRLRAARP 301
Query: 292 DVSITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKK 351
D+SIT+D IVGFPG ++ + + F YS R GT A +E+ K
Sbjct: 302 DISITSDFIVGFPGETEADFEATMKLIEAVNFDGSFSFIYSSRPGTPAAGLEDTTPHQVK 361
Query: 352 DERSKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNV 411
ER ++L E + +A + + +G VL E G +G T N VN +
Sbjct: 362 LERLQRLQEKVELQAQAISVRMVGTTQRVLVEGLSRKDPGELSGRTDNNRVVNFPGSPEM 421
Query: 412 SGKILNVKINSFEGEVAEGEIV 433
GK +KI + GE V
Sbjct: 422 IGKFAELKITAALSHTLRGENV 443
>MIAB_KLEP3 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 474
Score = 197 bits (501), Expect = 1e-52
Identities = 136/444 (30%), Positives = 218/444 (49%), Gaps = 18/444 (4%)
Query: 2 KVAFATLGCRVNTYESEAMTEKF-VREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S M + GY++ + E ADV ++NTCS+ +K +
Sbjct: 4 KLHIKTWGCQMNEYDSSKMADLLDATHGYQLTEVAEEADVLLLNTCSIREKAQEKVFHQL 63
Query: 61 GR---ARRQNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR + +NP+ II V GC A + VD++ G + + +N R
Sbjct: 64 GRWKLLKEKNPDLIIGVGGCVASQEGDHIRQRAHYVDIIFGPQTLHRLPEMINAVRGNRS 123
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
V + K F+ L + T AF+ I +GCN++C YC++PYTRG S+ +
Sbjct: 124 PVVDISFPEIEK-FDRLPEPRADGPT-AFVSIMEGCNKYCTYCVVPYTRGEEVSRPCDDI 181
Query: 177 LDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKV-TLVDLLEEIEKIDGIERVRIGSIDP 234
L EI LA G +E+ L G + ++ G + D + DLL + IDGI+R+R + P
Sbjct: 182 LFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSFADLLRLVAAIDGIDRIRFTTSHP 241
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
FT+D++ +L HL +QSG D L M R +TA EY+ I+ LR+ D+
Sbjct: 242 IEFTDDIIEVYRDTPELVSFLHLPVQSGSDRVLNLMGRTHTALEYKAIIRKLREARPDIQ 301
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
I++D IVGFPG + + ++ F +S R GT A +M + V ++D++
Sbjct: 302 ISSDFIVGFPGETTDDFEKTMKLIADVNFDMSYSFIFSARPGTPAADMVDDVP--EEDKK 359
Query: 355 SKKLIELNRINEKAFA--EKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVS 412
+ I RIN++A A + +G V +L E +G T N VN + ++
Sbjct: 360 QRLYILQERINQQAMAWSRRMLGTVQRILVEGTSRKSLMELSGRTENNRVVNFEGTPDMV 419
Query: 413 GKILNVKI-----NSFEGEVAEGE 431
GK ++V+I NS G++ E
Sbjct: 420 GKFVDVEIVDVYTNSLRGKIVRTE 443
>MIAB_ESCF3 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 474
Score = 197 bits (501), Expect = 1e-52
Identities = 139/444 (31%), Positives = 215/444 (48%), Gaps = 18/444 (4%)
Query: 2 KVAFATLGCRVNTYESEAMTEKF-VREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S M + GY++ D E ADV ++NTCS+ +K +
Sbjct: 4 KLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVLLLNTCSIREKAQEKVFHQL 63
Query: 61 GR---ARRQNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR + +NP+ II V GC A + VD++ G + + +N R +
Sbjct: 64 GRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDIIFGPQTLHRLPEMINSVRGDRS 123
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
V + K F+ L T AF+ I +GCN++C YC++PYTRG S+ +
Sbjct: 124 PVVDISFPEIEK-FDRLPEPRAEGPT-AFVSIMEGCNKYCTYCVVPYTRGEEVSRPSDDI 181
Query: 177 LDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKV-TLVDLLEEIEKIDGIERVRIGSIDP 234
L EI LA G +E+ L G + ++ G + D + DLL + IDGI+R+R + P
Sbjct: 182 LFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSFADLLRLVAAIDGIDRIRFTTSHP 241
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
FT+D++ +L HL +QSG D L M R +TA EY+ I+ LR D+
Sbjct: 242 IEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNLMGRTHTALEYKAIIRKLRAARPDIQ 301
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
I++D IVGFPG + + ++ F +S R GT A +M + V +K +R
Sbjct: 302 ISSDFIVGFPGETTEDFEKTMKLIADVNFDMSYSFIFSARPGTPAADMVDDVPEEEKKQR 361
Query: 355 SKKLIELNRINEKAFA--EKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVS 412
L E RIN++A A + +G +L E +G T N VN + ++
Sbjct: 362 LYILQE--RINQQAMAWSRRMLGTTQRILVEGTSRKSIMELSGRTENNRVVNFEGTPDMI 419
Query: 413 GKILNVKI-----NSFEGEVAEGE 431
GK ++V+I NS G+V E
Sbjct: 420 GKFVDVEITDVYPNSLRGKVVRTE 443
>MIAB_ECOUT RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 474
Score = 197 bits (501), Expect = 1e-52
Identities = 139/444 (31%), Positives = 215/444 (48%), Gaps = 18/444 (4%)
Query: 2 KVAFATLGCRVNTYESEAMTEKF-VREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S M + GY++ D E ADV ++NTCS+ +K +
Sbjct: 4 KLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVLLLNTCSIREKAQEKVFHQL 63
Query: 61 GR---ARRQNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR + +NP+ II V GC A + VD++ G + + +N R +
Sbjct: 64 GRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDIIFGPQTLHRLPEMINSVRGDRS 123
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
V + K F+ L T AF+ I +GCN++C YC++PYTRG S+ +
Sbjct: 124 PVVDISFPEIEK-FDRLPEPRAEGPT-AFVSIMEGCNKYCTYCVVPYTRGEEVSRPSDDI 181
Query: 177 LDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKV-TLVDLLEEIEKIDGIERVRIGSIDP 234
L EI LA G +E+ L G + ++ G + D + DLL + IDGI+R+R + P
Sbjct: 182 LFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSFADLLRLVAAIDGIDRIRFTTSHP 241
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
FT+D++ +L HL +QSG D L M R +TA EY+ I+ LR D+
Sbjct: 242 IEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNLMGRTHTALEYKAIIRKLRAARPDIQ 301
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
I++D IVGFPG + + ++ F +S R GT A +M + V +K +R
Sbjct: 302 ISSDFIVGFPGETTDDFEKTMKLIADVNFDMSYSFIFSARPGTPAADMVDDVPEEEKKQR 361
Query: 355 SKKLIELNRINEKAFA--EKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVS 412
L E RIN++A A + +G +L E +G T N VN + ++
Sbjct: 362 LYILQE--RINQQAMAWSRRMLGTTQRILVEGTSRKSIMELSGRTENNRVVNFEGTPDMI 419
Query: 413 GKILNVKI-----NSFEGEVAEGE 431
GK ++V+I NS G+V E
Sbjct: 420 GKFVDVEITDVYPNSLRGKVVRTE 443
>MIAB_ECOSM RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 474
Score = 197 bits (501), Expect = 1e-52
Identities = 139/444 (31%), Positives = 215/444 (48%), Gaps = 18/444 (4%)
Query: 2 KVAFATLGCRVNTYESEAMTEKF-VREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S M + GY++ D E ADV ++NTCS+ +K +
Sbjct: 4 KLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVLLLNTCSIREKAQEKVFHQL 63
Query: 61 GR---ARRQNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR + +NP+ II V GC A + VD++ G + + +N R +
Sbjct: 64 GRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDIIFGPQTLHRLPEMINSVRGDRS 123
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
V + K F+ L T AF+ I +GCN++C YC++PYTRG S+ +
Sbjct: 124 PVVDISFPEIEK-FDRLPEPRAEGPT-AFVSIMEGCNKYCTYCVVPYTRGEEVSRPSDDI 181
Query: 177 LDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKV-TLVDLLEEIEKIDGIERVRIGSIDP 234
L EI LA G +E+ L G + ++ G + D + DLL + IDGI+R+R + P
Sbjct: 182 LFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSFADLLRLVAAIDGIDRIRFTTSHP 241
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
FT+D++ +L HL +QSG D L M R +TA EY+ I+ LR D+
Sbjct: 242 IEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNLMGRTHTALEYKAIIRKLRAARPDIQ 301
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
I++D IVGFPG + + ++ F +S R GT A +M + V +K +R
Sbjct: 302 ISSDFIVGFPGETTEDFEKTMKLIADVNFDMSYSFIFSARPGTPAADMVDDVPEEEKKQR 361
Query: 355 SKKLIELNRINEKAFA--EKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVS 412
L E RIN++A A + +G +L E +G T N VN + ++
Sbjct: 362 LYILQE--RINQQAMAWSRRMLGTTQRILVEGTSRKSIMELSGRTENNRVVNFEGTPDMI 419
Query: 413 GKILNVKI-----NSFEGEVAEGE 431
GK ++V+I NS G+V E
Sbjct: 420 GKFVDVEITDVYPNSLRGKVVRTE 443
>MIAB_ECOSE RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 474
Score = 197 bits (501), Expect = 1e-52
Identities = 139/444 (31%), Positives = 215/444 (48%), Gaps = 18/444 (4%)
Query: 2 KVAFATLGCRVNTYESEAMTEKF-VREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S M + GY++ D E ADV ++NTCS+ +K +
Sbjct: 4 KLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVLLLNTCSIREKAQEKVFHQL 63
Query: 61 GR---ARRQNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR + +NP+ II V GC A + VD++ G + + +N R +
Sbjct: 64 GRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDIIFGPQTLHRLPEMINSVRGDRS 123
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
V + K F+ L T AF+ I +GCN++C YC++PYTRG S+ +
Sbjct: 124 PVVDISFPEIEK-FDRLPEPRAEGPT-AFVSIMEGCNKYCTYCVVPYTRGEEVSRPSDDI 181
Query: 177 LDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKV-TLVDLLEEIEKIDGIERVRIGSIDP 234
L EI LA G +E+ L G + ++ G + D + DLL + IDGI+R+R + P
Sbjct: 182 LFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSFADLLRLVAAIDGIDRIRFTTSHP 241
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
FT+D++ +L HL +QSG D L M R +TA EY+ I+ LR D+
Sbjct: 242 IEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNLMGRTHTALEYKAIIRKLRAARPDIQ 301
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
I++D IVGFPG + + ++ F +S R GT A +M + V +K +R
Sbjct: 302 ISSDFIVGFPGETTEDFEKTMKLIADVNFDMSYSFIFSARPGTPAADMVDDVPEEEKKQR 361
Query: 355 SKKLIELNRINEKAFA--EKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVS 412
L E RIN++A A + +G +L E +G T N VN + ++
Sbjct: 362 LYILQE--RINQQAMAWSRRMLGTTQRILVEGTSRKSIMELSGRTENNRVVNFEGTPDMI 419
Query: 413 GKILNVKI-----NSFEGEVAEGE 431
GK ++V+I NS G+V E
Sbjct: 420 GKFVDVEITDVYPNSLRGKVVRTE 443
>MIAB_ECOLU RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 474
Score = 197 bits (501), Expect = 1e-52
Identities = 139/444 (31%), Positives = 215/444 (48%), Gaps = 18/444 (4%)
Query: 2 KVAFATLGCRVNTYESEAMTEKF-VREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S M + GY++ D E ADV ++NTCS+ +K +
Sbjct: 4 KLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVLLLNTCSIREKAQEKVFHQL 63
Query: 61 GR---ARRQNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR + +NP+ II V GC A + VD++ G + + +N R +
Sbjct: 64 GRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDIIFGPQTLHRLPEMINSVRGDRS 123
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
V + K F+ L T AF+ I +GCN++C YC++PYTRG S+ +
Sbjct: 124 PVVDISFPEIEK-FDRLPEPRAEGPT-AFVSIMEGCNKYCTYCVVPYTRGEEVSRPSDDI 181
Query: 177 LDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKV-TLVDLLEEIEKIDGIERVRIGSIDP 234
L EI LA G +E+ L G + ++ G + D + DLL + IDGI+R+R + P
Sbjct: 182 LFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSFADLLRLVAAIDGIDRIRFTTSHP 241
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
FT+D++ +L HL +QSG D L M R +TA EY+ I+ LR D+
Sbjct: 242 IEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNLMGRTHTALEYKAIIRKLRAARPDIQ 301
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
I++D IVGFPG + + ++ F +S R GT A +M + V +K +R
Sbjct: 302 ISSDFIVGFPGETTEDFEKTMKLIADVNFDMSYSFIFSARPGTPAADMVDDVPEEEKKQR 361
Query: 355 SKKLIELNRINEKAFA--EKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVS 412
L E RIN++A A + +G +L E +G T N VN + ++
Sbjct: 362 LYILQE--RINQQAMAWSRRMLGTTQRILVEGTSRKSIMELSGRTENNRVVNFEGTPDMI 419
Query: 413 GKILNVKI-----NSFEGEVAEGE 431
GK ++V+I NS G+V E
Sbjct: 420 GKFVDVEITDVYPNSLRGKVVRTE 443
>MIAB_ECOLI RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 474
Score = 197 bits (501), Expect = 1e-52
Identities = 139/444 (31%), Positives = 215/444 (48%), Gaps = 18/444 (4%)
Query: 2 KVAFATLGCRVNTYESEAMTEKF-VREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S M + GY++ D E ADV ++NTCS+ +K +
Sbjct: 4 KLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVLLLNTCSIREKAQEKVFHQL 63
Query: 61 GR---ARRQNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR + +NP+ II V GC A + VD++ G + + +N R +
Sbjct: 64 GRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDIIFGPQTLHRLPEMINSVRGDRS 123
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
V + K F+ L T AF+ I +GCN++C YC++PYTRG S+ +
Sbjct: 124 PVVDISFPEIEK-FDRLPEPRAEGPT-AFVSIMEGCNKYCTYCVVPYTRGEEVSRPSDDI 181
Query: 177 LDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKV-TLVDLLEEIEKIDGIERVRIGSIDP 234
L EI LA G +E+ L G + ++ G + D + DLL + IDGI+R+R + P
Sbjct: 182 LFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSFADLLRLVAAIDGIDRIRFTTSHP 241
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
FT+D++ +L HL +QSG D L M R +TA EY+ I+ LR D+
Sbjct: 242 IEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNLMGRTHTALEYKAIIRKLRAARPDIQ 301
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
I++D IVGFPG + + ++ F +S R GT A +M + V +K +R
Sbjct: 302 ISSDFIVGFPGETTEDFEKTMKLIADVNFDMSYSFIFSARPGTPAADMVDDVPEEEKKQR 361
Query: 355 SKKLIELNRINEKAFA--EKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVS 412
L E RIN++A A + +G +L E +G T N VN + ++
Sbjct: 362 LYILQE--RINQQAMAWSRRMLGTTQRILVEGTSRKSIMELSGRTENNRVVNFEGTPDMI 419
Query: 413 GKILNVKI-----NSFEGEVAEGE 431
GK ++V+I NS G+V E
Sbjct: 420 GKFVDVEITDVYPNSLRGKVVRTE 443
>MIAB_ECOLC RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 474
Score = 197 bits (501), Expect = 1e-52
Identities = 139/444 (31%), Positives = 215/444 (48%), Gaps = 18/444 (4%)
Query: 2 KVAFATLGCRVNTYESEAMTEKF-VREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S M + GY++ D E ADV ++NTCS+ +K +
Sbjct: 4 KLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVLLLNTCSIREKAQEKVFHQL 63
Query: 61 GR---ARRQNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR + +NP+ II V GC A + VD++ G + + +N R +
Sbjct: 64 GRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDIIFGPQTLHRLPEMINSVRGDRS 123
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
V + K F+ L T AF+ I +GCN++C YC++PYTRG S+ +
Sbjct: 124 PVVDISFPEIEK-FDRLPEPRAEGPT-AFVSIMEGCNKYCTYCVVPYTRGEEVSRPSDDI 181
Query: 177 LDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKV-TLVDLLEEIEKIDGIERVRIGSIDP 234
L EI LA G +E+ L G + ++ G + D + DLL + IDGI+R+R + P
Sbjct: 182 LFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSFADLLRLVAAIDGIDRIRFTTSHP 241
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
FT+D++ +L HL +QSG D L M R +TA EY+ I+ LR D+
Sbjct: 242 IEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNLMGRTHTALEYKAIIRKLRAARPDIQ 301
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
I++D IVGFPG + + ++ F +S R GT A +M + V +K +R
Sbjct: 302 ISSDFIVGFPGETTEDFEKTMKLIADVNFDMSYSFIFSARPGTPAADMVDDVPEEEKKQR 361
Query: 355 SKKLIELNRINEKAFA--EKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVS 412
L E RIN++A A + +G +L E +G T N VN + ++
Sbjct: 362 LYILQE--RINQQAMAWSRRMLGTTQRILVEGTSRKSIMELSGRTENNRVVNFEGTPDMI 419
Query: 413 GKILNVKI-----NSFEGEVAEGE 431
GK ++V+I NS G+V E
Sbjct: 420 GKFVDVEITDVYPNSLRGKVVRTE 443
>MIAB_ECOL6 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 474
Score = 197 bits (501), Expect = 1e-52
Identities = 139/444 (31%), Positives = 215/444 (48%), Gaps = 18/444 (4%)
Query: 2 KVAFATLGCRVNTYESEAMTEKF-VREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S M + GY++ D E ADV ++NTCS+ +K +
Sbjct: 4 KLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVLLLNTCSIREKAQEKVFHQL 63
Query: 61 GR---ARRQNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR + +NP+ II V GC A + VD++ G + + +N R +
Sbjct: 64 GRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDIIFGPQTLHRLPEMINSVRGDRS 123
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
V + K F+ L T AF+ I +GCN++C YC++PYTRG S+ +
Sbjct: 124 PVVDISFPEIEK-FDRLPEPRAEGPT-AFVSIMEGCNKYCTYCVVPYTRGEEVSRPSDDI 181
Query: 177 LDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKV-TLVDLLEEIEKIDGIERVRIGSIDP 234
L EI LA G +E+ L G + ++ G + D + DLL + IDGI+R+R + P
Sbjct: 182 LFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSFADLLRLVAAIDGIDRIRFTTSHP 241
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
FT+D++ +L HL +QSG D L M R +TA EY+ I+ LR D+
Sbjct: 242 IEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNLMGRTHTALEYKAIIRKLRAARPDIQ 301
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
I++D IVGFPG + + ++ F +S R GT A +M + V +K +R
Sbjct: 302 ISSDFIVGFPGETTEDFEKTMKLIADVNFDMSYSFIFSARPGTPAADMVDDVPEEEKKQR 361
Query: 355 SKKLIELNRINEKAFA--EKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVS 412
L E RIN++A A + +G +L E +G T N VN + ++
Sbjct: 362 LYILQE--RINQQAMAWSRRMLGTTQRILVEGTSRKSIMELSGRTENNRVVNFEGTPDMI 419
Query: 413 GKILNVKI-----NSFEGEVAEGE 431
GK ++V+I NS G+V E
Sbjct: 420 GKFVDVEITDVYPNSLRGKVVRTE 443
>MIAB_ECOL5 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 474
Score = 197 bits (501), Expect = 1e-52
Identities = 139/444 (31%), Positives = 215/444 (48%), Gaps = 18/444 (4%)
Query: 2 KVAFATLGCRVNTYESEAMTEKF-VREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S M + GY++ D E ADV ++NTCS+ +K +
Sbjct: 4 KLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVLLLNTCSIREKAQEKVFHQL 63
Query: 61 GR---ARRQNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR + +NP+ II V GC A + VD++ G + + +N R +
Sbjct: 64 GRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDIIFGPQTLHRLPEMINSVRGDRS 123
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
V + K F+ L T AF+ I +GCN++C YC++PYTRG S+ +
Sbjct: 124 PVVDISFPEIEK-FDRLPEPRAEGPT-AFVSIMEGCNKYCTYCVVPYTRGEEVSRPSDDI 181
Query: 177 LDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKV-TLVDLLEEIEKIDGIERVRIGSIDP 234
L EI LA G +E+ L G + ++ G + D + DLL + IDGI+R+R + P
Sbjct: 182 LFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSFADLLRLVAAIDGIDRIRFTTSHP 241
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
FT+D++ +L HL +QSG D L M R +TA EY+ I+ LR D+
Sbjct: 242 IEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNLMGRTHTALEYKAIIRKLRAARPDIQ 301
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
I++D IVGFPG + + ++ F +S R GT A +M + V +K +R
Sbjct: 302 ISSDFIVGFPGETTEDFEKTMKLIADVNFDMSYSFIFSARPGTPAADMVDDVPEEEKKQR 361
Query: 355 SKKLIELNRINEKAFA--EKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVS 412
L E RIN++A A + +G +L E +G T N VN + ++
Sbjct: 362 LYILQE--RINQQAMAWSRRMLGTTQRILVEGTSRKSIMELSGRTENNRVVNFEGTPDMI 419
Query: 413 GKILNVKI-----NSFEGEVAEGE 431
GK ++V+I NS G+V E
Sbjct: 420 GKFVDVEITDVYPNSLRGKVVRTE 443
>MIAB_ECOK1 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 474
Score = 197 bits (501), Expect = 1e-52
Identities = 139/444 (31%), Positives = 215/444 (48%), Gaps = 18/444 (4%)
Query: 2 KVAFATLGCRVNTYESEAMTEKF-VREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S M + GY++ D E ADV ++NTCS+ +K +
Sbjct: 4 KLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVLLLNTCSIREKAQEKVFHQL 63
Query: 61 GR---ARRQNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR + +NP+ II V GC A + VD++ G + + +N R +
Sbjct: 64 GRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDIIFGPQTLHRLPEMINSVRGDRS 123
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
V + K F+ L T AF+ I +GCN++C YC++PYTRG S+ +
Sbjct: 124 PVVDISFPEIEK-FDRLPEPRAEGPT-AFVSIMEGCNKYCTYCVVPYTRGEEVSRPSDDI 181
Query: 177 LDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKV-TLVDLLEEIEKIDGIERVRIGSIDP 234
L EI LA G +E+ L G + ++ G + D + DLL + IDGI+R+R + P
Sbjct: 182 LFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSFADLLRLVAAIDGIDRIRFTTSHP 241
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
FT+D++ +L HL +QSG D L M R +TA EY+ I+ LR D+
Sbjct: 242 IEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNLMGRTHTALEYKAIIRKLRAARPDIQ 301
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
I++D IVGFPG + + ++ F +S R GT A +M + V +K +R
Sbjct: 302 ISSDFIVGFPGETTDDFEKTMKLIADVNFDMSYSFIFSARPGTPAADMVDDVPEEEKKQR 361
Query: 355 SKKLIELNRINEKAFA--EKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVS 412
L E RIN++A A + +G +L E +G T N VN + ++
Sbjct: 362 LYILQE--RINQQAMAWSRRMLGTTQRILVEGTSRKSIMELSGRTENNRVVNFEGTPDMI 419
Query: 413 GKILNVKI-----NSFEGEVAEGE 431
GK ++V+I NS G+V E
Sbjct: 420 GKFVDVEITDVYPNSLRGKVVRTE 443
>MIAB_ECOHS RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 474
Score = 197 bits (501), Expect = 1e-52
Identities = 139/444 (31%), Positives = 215/444 (48%), Gaps = 18/444 (4%)
Query: 2 KVAFATLGCRVNTYESEAMTEKF-VREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S M + GY++ D E ADV ++NTCS+ +K +
Sbjct: 4 KLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVLLLNTCSIREKAQEKVFHQL 63
Query: 61 GR---ARRQNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR + +NP+ II V GC A + VD++ G + + +N R +
Sbjct: 64 GRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDIIFGPQTLHRLPEMINSVRGDRS 123
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
V + K F+ L T AF+ I +GCN++C YC++PYTRG S+ +
Sbjct: 124 PVVDISFPEIEK-FDRLPEPRAEGPT-AFVSIMEGCNKYCTYCVVPYTRGEEVSRPSDDI 181
Query: 177 LDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKV-TLVDLLEEIEKIDGIERVRIGSIDP 234
L EI LA G +E+ L G + ++ G + D + DLL + IDGI+R+R + P
Sbjct: 182 LFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSFADLLRLVAAIDGIDRIRFTTSHP 241
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
FT+D++ +L HL +QSG D L M R +TA EY+ I+ LR D+
Sbjct: 242 IEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNLMGRTHTALEYKAIIRKLRAARPDIQ 301
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
I++D IVGFPG + + ++ F +S R GT A +M + V +K +R
Sbjct: 302 ISSDFIVGFPGETTEDFEKTMKLIADVNFDMSYSFIFSARPGTPAADMVDDVPEEEKKQR 361
Query: 355 SKKLIELNRINEKAFA--EKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVS 412
L E RIN++A A + +G +L E +G T N VN + ++
Sbjct: 362 LYILQE--RINQQAMAWSRRMLGTTQRILVEGTSRKSIMELSGRTENNRVVNFEGTPDMI 419
Query: 413 GKILNVKI-----NSFEGEVAEGE 431
GK ++V+I NS G+V E
Sbjct: 420 GKFVDVEITDVYPNSLRGKVVRTE 443
>MIAB_ECODH RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 474
Score = 197 bits (501), Expect = 1e-52
Identities = 139/444 (31%), Positives = 215/444 (48%), Gaps = 18/444 (4%)
Query: 2 KVAFATLGCRVNTYESEAMTEKF-VREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S M + GY++ D E ADV ++NTCS+ +K +
Sbjct: 4 KLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVLLLNTCSIREKAQEKVFHQL 63
Query: 61 GR---ARRQNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR + +NP+ II V GC A + VD++ G + + +N R +
Sbjct: 64 GRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDIIFGPQTLHRLPEMINSVRGDRS 123
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
V + K F+ L T AF+ I +GCN++C YC++PYTRG S+ +
Sbjct: 124 PVVDISFPEIEK-FDRLPEPRAEGPT-AFVSIMEGCNKYCTYCVVPYTRGEEVSRPSDDI 181
Query: 177 LDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKV-TLVDLLEEIEKIDGIERVRIGSIDP 234
L EI LA G +E+ L G + ++ G + D + DLL + IDGI+R+R + P
Sbjct: 182 LFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSFADLLRLVAAIDGIDRIRFTTSHP 241
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
FT+D++ +L HL +QSG D L M R +TA EY+ I+ LR D+
Sbjct: 242 IEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNLMGRTHTALEYKAIIRKLRAARPDIQ 301
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
I++D IVGFPG + + ++ F +S R GT A +M + V +K +R
Sbjct: 302 ISSDFIVGFPGETTEDFEKTMKLIADVNFDMSYSFIFSARPGTPAADMVDDVPEEEKKQR 361
Query: 355 SKKLIELNRINEKAFA--EKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVS 412
L E RIN++A A + +G +L E +G T N VN + ++
Sbjct: 362 LYILQE--RINQQAMAWSRRMLGTTQRILVEGTSRKSIMELSGRTENNRVVNFEGTPDMI 419
Query: 413 GKILNVKI-----NSFEGEVAEGE 431
GK ++V+I NS G+V E
Sbjct: 420 GKFVDVEITDVYPNSLRGKVVRTE 443
>MIAB_ECO8A RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 474
Score = 197 bits (501), Expect = 1e-52
Identities = 139/444 (31%), Positives = 215/444 (48%), Gaps = 18/444 (4%)
Query: 2 KVAFATLGCRVNTYESEAMTEKF-VREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S M + GY++ D E ADV ++NTCS+ +K +
Sbjct: 4 KLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVLLLNTCSIREKAQEKVFHQL 63
Query: 61 GR---ARRQNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR + +NP+ II V GC A + VD++ G + + +N R +
Sbjct: 64 GRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDIIFGPQTLHRLPEMINSVRGDRS 123
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
V + K F+ L T AF+ I +GCN++C YC++PYTRG S+ +
Sbjct: 124 PVVDISFPEIEK-FDRLPEPRAEGPT-AFVSIMEGCNKYCTYCVVPYTRGEEVSRPSDDI 181
Query: 177 LDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKV-TLVDLLEEIEKIDGIERVRIGSIDP 234
L EI LA G +E+ L G + ++ G + D + DLL + IDGI+R+R + P
Sbjct: 182 LFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSFADLLRLVAAIDGIDRIRFTTSHP 241
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
FT+D++ +L HL +QSG D L M R +TA EY+ I+ LR D+
Sbjct: 242 IEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNLMGRTHTALEYKAIIRKLRAARPDIQ 301
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
I++D IVGFPG + + ++ F +S R GT A +M + V +K +R
Sbjct: 302 ISSDFIVGFPGETTEDFEKTMKLIADVNFDMSYSFIFSARPGTPAADMVDDVPEEEKKQR 361
Query: 355 SKKLIELNRINEKAFA--EKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVS 412
L E RIN++A A + +G +L E +G T N VN + ++
Sbjct: 362 LYILQE--RINQQAMAWSRRMLGTTQRILVEGTSRKSIMELSGRTENNRVVNFEGTPDMI 419
Query: 413 GKILNVKI-----NSFEGEVAEGE 431
GK ++V+I NS G+V E
Sbjct: 420 GKFVDVEITDVYPNSLRGKVVRTE 443
>MIAB_ECO7I RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 474
Score = 197 bits (501), Expect = 1e-52
Identities = 139/444 (31%), Positives = 215/444 (48%), Gaps = 18/444 (4%)
Query: 2 KVAFATLGCRVNTYESEAMTEKF-VREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S M + GY++ D E ADV ++NTCS+ +K +
Sbjct: 4 KLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVLLLNTCSIREKAQEKVFHQL 63
Query: 61 GR---ARRQNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR + +NP+ II V GC A + VD++ G + + +N R +
Sbjct: 64 GRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDIIFGPQTLHRLPEMINSVRGDRS 123
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
V + K F+ L T AF+ I +GCN++C YC++PYTRG S+ +
Sbjct: 124 PVVDISFPEIEK-FDRLPEPRAEGPT-AFVSIMEGCNKYCTYCVVPYTRGEEVSRPSDDI 181
Query: 177 LDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKV-TLVDLLEEIEKIDGIERVRIGSIDP 234
L EI LA G +E+ L G + ++ G + D + DLL + IDGI+R+R + P
Sbjct: 182 LFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSFADLLRLVAAIDGIDRIRFTTSHP 241
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
FT+D++ +L HL +QSG D L M R +TA EY+ I+ LR D+
Sbjct: 242 IEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNLMGRTHTALEYKAIIRKLRAARPDIQ 301
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
I++D IVGFPG + + ++ F +S R GT A +M + V +K +R
Sbjct: 302 ISSDFIVGFPGETTEDFEKTMKLIADVNFDMSYSFIFSARPGTPAADMVDDVPEEEKKQR 361
Query: 355 SKKLIELNRINEKAFA--EKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVS 412
L E RIN++A A + +G +L E +G T N VN + ++
Sbjct: 362 LYILQE--RINQQAMAWSRRMLGTTQRILVEGTSRKSIMELSGRTENNRVVNFEGTPDMI 419
Query: 413 GKILNVKI-----NSFEGEVAEGE 431
GK ++V+I NS G+V E
Sbjct: 420 GKFVDVEITDVYPNSLRGKVVRTE 443
>MIAB_ECO5E RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 474
Score = 197 bits (501), Expect = 1e-52
Identities = 139/444 (31%), Positives = 215/444 (48%), Gaps = 18/444 (4%)
Query: 2 KVAFATLGCRVNTYESEAMTEKF-VREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S M + GY++ D E ADV ++NTCS+ +K +
Sbjct: 4 KLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVLLLNTCSIREKAQEKVFHQL 63
Query: 61 GR---ARRQNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR + +NP+ II V GC A + VD++ G + + +N R +
Sbjct: 64 GRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDIIFGPQTLHRLPEMINSVRGDRS 123
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
V + K F+ L T AF+ I +GCN++C YC++PYTRG S+ +
Sbjct: 124 PVVDISFPEIEK-FDRLPEPRAEGPT-AFVSIMEGCNKYCTYCVVPYTRGEEVSRPSDDI 181
Query: 177 LDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKV-TLVDLLEEIEKIDGIERVRIGSIDP 234
L EI LA G +E+ L G + ++ G + D + DLL + IDGI+R+R + P
Sbjct: 182 LFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSFADLLRLVAAIDGIDRIRFTTSHP 241
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
FT+D++ +L HL +QSG D L M R +TA EY+ I+ LR D+
Sbjct: 242 IEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNLMGRTHTALEYKAIIRKLRAARPDIQ 301
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
I++D IVGFPG + + ++ F +S R GT A +M + V +K +R
Sbjct: 302 ISSDFIVGFPGETTEDFEKTMKLIADVNFDMSYSFIFSARPGTPAADMVDDVPEEEKKQR 361
Query: 355 SKKLIELNRINEKAFA--EKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVS 412
L E RIN++A A + +G +L E +G T N VN + ++
Sbjct: 362 LYILQE--RINQQAMAWSRRMLGTTQRILVEGTSRKSIMELSGRTENNRVVNFEGTPDMI 419
Query: 413 GKILNVKI-----NSFEGEVAEGE 431
GK ++V+I NS G+V E
Sbjct: 420 GKFVDVEITDVYPNSLRGKVVRTE 443
>MIAB_ECO57 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 474
Score = 197 bits (501), Expect = 1e-52
Identities = 139/444 (31%), Positives = 215/444 (48%), Gaps = 18/444 (4%)
Query: 2 KVAFATLGCRVNTYESEAMTEKF-VREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S M + GY++ D E ADV ++NTCS+ +K +
Sbjct: 4 KLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVLLLNTCSIREKAQEKVFHQL 63
Query: 61 GR---ARRQNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR + +NP+ II V GC A + VD++ G + + +N R +
Sbjct: 64 GRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDIIFGPQTLHRLPEMINSVRGDRS 123
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
V + K F+ L T AF+ I +GCN++C YC++PYTRG S+ +
Sbjct: 124 PVVDISFPEIEK-FDRLPEPRAEGPT-AFVSIMEGCNKYCTYCVVPYTRGEEVSRPSDDI 181
Query: 177 LDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKV-TLVDLLEEIEKIDGIERVRIGSIDP 234
L EI LA G +E+ L G + ++ G + D + DLL + IDGI+R+R + P
Sbjct: 182 LFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSFADLLRLVAAIDGIDRIRFTTSHP 241
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
FT+D++ +L HL +QSG D L M R +TA EY+ I+ LR D+
Sbjct: 242 IEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNLMGRTHTALEYKAIIRKLRAARPDIQ 301
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
I++D IVGFPG + + ++ F +S R GT A +M + V +K +R
Sbjct: 302 ISSDFIVGFPGETTEDFEKTMKLIADVNFDMSYSFIFSARPGTPAADMVDDVPEEEKKQR 361
Query: 355 SKKLIELNRINEKAFA--EKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVS 412
L E RIN++A A + +G +L E +G T N VN + ++
Sbjct: 362 LYILQE--RINQQAMAWSRRMLGTTQRILVEGTSRKSIMELSGRTENNRVVNFEGTPDMI 419
Query: 413 GKILNVKI-----NSFEGEVAEGE 431
GK ++V+I NS G+V E
Sbjct: 420 GKFVDVEITDVYPNSLRGKVVRTE 443
>MIAB_ECO55 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 474
Score = 197 bits (501), Expect = 1e-52
Identities = 139/444 (31%), Positives = 215/444 (48%), Gaps = 18/444 (4%)
Query: 2 KVAFATLGCRVNTYESEAMTEKF-VREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S M + GY++ D E ADV ++NTCS+ +K +
Sbjct: 4 KLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVLLLNTCSIREKAQEKVFHQL 63
Query: 61 GR---ARRQNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR + +NP+ II V GC A + VD++ G + + +N R +
Sbjct: 64 GRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDIIFGPQTLHRLPEMINSVRGDRS 123
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
V + K F+ L T AF+ I +GCN++C YC++PYTRG S+ +
Sbjct: 124 PVVDISFPEIEK-FDRLPEPRAEGPT-AFVSIMEGCNKYCTYCVVPYTRGEEVSRPSDDI 181
Query: 177 LDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKV-TLVDLLEEIEKIDGIERVRIGSIDP 234
L EI LA G +E+ L G + ++ G + D + DLL + IDGI+R+R + P
Sbjct: 182 LFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSFADLLRLVAAIDGIDRIRFTTSHP 241
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
FT+D++ +L HL +QSG D L M R +TA EY+ I+ LR D+
Sbjct: 242 IEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNLMGRTHTALEYKAIIRKLRAARPDIQ 301
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
I++D IVGFPG + + ++ F +S R GT A +M + V +K +R
Sbjct: 302 ISSDFIVGFPGETTEDFEKTMKLIADVNFDMSYSFIFSARPGTPAADMVDDVPEEEKKQR 361
Query: 355 SKKLIELNRINEKAFA--EKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVS 412
L E RIN++A A + +G +L E +G T N VN + ++
Sbjct: 362 LYILQE--RINQQAMAWSRRMLGTTQRILVEGTSRKSIMELSGRTENNRVVNFEGTPDMI 419
Query: 413 GKILNVKI-----NSFEGEVAEGE 431
GK ++V+I NS G+V E
Sbjct: 420 GKFVDVEITDVYPNSLRGKVVRTE 443
>MIAB_ECO45 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 474
Score = 197 bits (501), Expect = 1e-52
Identities = 139/444 (31%), Positives = 215/444 (48%), Gaps = 18/444 (4%)
Query: 2 KVAFATLGCRVNTYESEAMTEKF-VREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S M + GY++ D E ADV ++NTCS+ +K +
Sbjct: 4 KLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVLLLNTCSIREKAQEKVFHQL 63
Query: 61 GR---ARRQNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR + +NP+ II V GC A + VD++ G + + +N R +
Sbjct: 64 GRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDIIFGPQTLHRLPEMINSVRGDRS 123
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
V + K F+ L T AF+ I +GCN++C YC++PYTRG S+ +
Sbjct: 124 PVVDISFPEIEK-FDRLPEPRAEGPT-AFVSIMEGCNKYCTYCVVPYTRGEEVSRPSDDI 181
Query: 177 LDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKV-TLVDLLEEIEKIDGIERVRIGSIDP 234
L EI LA G +E+ L G + ++ G + D + DLL + IDGI+R+R + P
Sbjct: 182 LFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSFADLLRLVAAIDGIDRIRFTTSHP 241
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
FT+D++ +L HL +QSG D L M R +TA EY+ I+ LR D+
Sbjct: 242 IEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNLMGRTHTALEYKAIIRKLRAARPDIQ 301
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
I++D IVGFPG + + ++ F +S R GT A +M + V +K +R
Sbjct: 302 ISSDFIVGFPGETTDDFEKTMKLIADVNFDMSYSFIFSARPGTPAADMVDDVPEEEKKQR 361
Query: 355 SKKLIELNRINEKAFA--EKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVS 412
L E RIN++A A + +G +L E +G T N VN + ++
Sbjct: 362 LYILQE--RINQQAMAWSRRMLGTTQRILVEGTSRKSIMELSGRTENNRVVNFEGTPDMI 419
Query: 413 GKILNVKI-----NSFEGEVAEGE 431
GK ++V+I NS G+V E
Sbjct: 420 GKFVDVEITDVYPNSLRGKVVRTE 443
>MIAB_ECO24 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 474
Score = 197 bits (501), Expect = 1e-52
Identities = 139/444 (31%), Positives = 215/444 (48%), Gaps = 18/444 (4%)
Query: 2 KVAFATLGCRVNTYESEAMTEKF-VREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S M + GY++ D E ADV ++NTCS+ +K +
Sbjct: 4 KLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVLLLNTCSIREKAQEKVFHQL 63
Query: 61 GR---ARRQNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR + +NP+ II V GC A + VD++ G + + +N R +
Sbjct: 64 GRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDIIFGPQTLHRLPEMINSVRGDRS 123
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
V + K F+ L T AF+ I +GCN++C YC++PYTRG S+ +
Sbjct: 124 PVVDISFPEIEK-FDRLPEPRAEGPT-AFVSIMEGCNKYCTYCVVPYTRGEEVSRPSDDI 181
Query: 177 LDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKV-TLVDLLEEIEKIDGIERVRIGSIDP 234
L EI LA G +E+ L G + ++ G + D + DLL + IDGI+R+R + P
Sbjct: 182 LFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSFADLLRLVAAIDGIDRIRFTTSHP 241
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
FT+D++ +L HL +QSG D L M R +TA EY+ I+ LR D+
Sbjct: 242 IEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNLMGRTHTALEYKAIIRKLRAARPDIQ 301
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
I++D IVGFPG + + ++ F +S R GT A +M + V +K +R
Sbjct: 302 ISSDFIVGFPGETTEDFEKTMKLIADVNFDMSYSFIFSARPGTPAADMVDDVPEEEKKQR 361
Query: 355 SKKLIELNRINEKAFA--EKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVS 412
L E RIN++A A + +G +L E +G T N VN + ++
Sbjct: 362 LYILQE--RINQQAMAWSRRMLGTTQRILVEGTSRKSIMELSGRTENNRVVNFEGTPDMI 419
Query: 413 GKILNVKI-----NSFEGEVAEGE 431
GK ++V+I NS G+V E
Sbjct: 420 GKFVDVEITDVYPNSLRGKVVRTE 443
>MIAB_POLNS RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 448
Score = 197 bits (500), Expect = 2e-52
Identities = 133/443 (30%), Positives = 217/443 (48%), Gaps = 13/443 (2%)
Query: 2 KVAFATLGCRVNTYESEAMTEKF-VREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S M + EG + D E ADV ++NTCS+ + K +
Sbjct: 3 KLYIKTFGCQMNEYDSGKMADLLHADEGMVMTDRPEDADVVLLNTCSIREKAEDKVFSDL 62
Query: 61 GRAR---RQNPEAIIAVVGCYA-QIAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR R + P +I V GC A Q VS VDVV G + + + K R+ G
Sbjct: 63 GRLRELKKTKPNLLIGVGGCVASQEGQQIVSHAPYVDVVFGPQTLHRLSDLIAKRRETGL 122
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
QV + K F+ L + A++ I +GC+++C+YC++PYTRG S+ V
Sbjct: 123 FQVDISFPEIEK-FDHLPASR-QTRGSAYVSIMEGCSKYCSYCVVPYTRGEEVSRSFDDV 180
Query: 177 LDEIRSLAEHGFKEIILSGIHTASYGVDLDEKVTLVD---LLEEIEKIDGIERVRIGSID 233
L E+ LA G KEI+L G + +Y + ++ + D L+E I +I G+ER+R +
Sbjct: 181 LTEVAGLASKGVKEIVLLGQNVNAYLGKMGDRQEIADFALLIEYIVEIPGVERIRFTTSH 240
Query: 234 PTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDV 293
P FT+ ++ + KL H HL +Q G DA L M R YTA E++ I+ +R D+
Sbjct: 241 PKEFTQRLIDVYTKVPKLVSHLHLPVQHGSDAMLSAMKRGYTALEFKSIIRKMRAVRPDL 300
Query: 294 SITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDE 353
++++D IVGFPG ++ + + F +SPR GT A N+ + K +
Sbjct: 301 TLSSDFIVGFPGETEADFEKLLKMVEELNFDNSFCFIFSPRPGTPAANLHDDTPYEVKLK 360
Query: 354 RSKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRN---YIKVNAKADCN 410
R + L+ L ++K +G + VL E + G + N + A+ N
Sbjct: 361 RLQTLLALVESQANQISQKMLGNIERVLIEGLAKDGINLQGRAENNRVIHFTAPAQNIEN 420
Query: 411 VSGKILNVKINSFEGEVAEGEIV 433
+ G++++++I G++V
Sbjct: 421 LIGQMVDIRITEVLNYTLRGKLV 443
>MIAB_PELCD RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 438
Score = 197 bits (500), Expect = 2e-52
Identities = 124/429 (28%), Positives = 211/429 (49%), Gaps = 9/429 (2%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIGR---A 63
T GC++N +SE + GY VD E A++ ++NTCS+ ++K +GR
Sbjct: 8 TFGCQMNVVDSEQIVGLVQSLGYSSVDSPEQANLIILNTCSIRARAERKVYGHLGRFKPL 67
Query: 64 RRQNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVKVG 122
+++ PE IIAV GC AQ + + +D+V G+ N + V A + V+V
Sbjct: 68 KQRRPELIIAVCGCVAQQEGQRMLEKVPYLDIVCGTHNIHRLADMVRDAELHRARHVEVD 127
Query: 123 AVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDEIRS 182
+ +K + + F+ + GC+ FC+YC++P+ RG S+
Sbjct: 128 FLEADKRRRLFPERAPSAEVSRFVTVIQGCDNFCSYCIVPHVRGREVSRPSAXXXXXXXX 187
Query: 183 LAEHGFKEIILSGIHTASYGVDLDEKVTLVDLLEEIEKIDGIERVRIGSIDPTFFTEDVV 242
G +EI L G + SYG D++++ LL ++ ++DG+ER+R + P +++++
Sbjct: 188 XXXQGAREITLIGQNVNSYGCKEDDEISFASLLRKVAEVDGLERIRFMTSHPKDLSDELI 247
Query: 243 RRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITTDVIVG 302
L KLC H HL +Q+G DA LK M R YT +Y + LR ++ +T+DVIVG
Sbjct: 248 DCFADLDKLCKHIHLPVQAGGDAVLKAMRRGYTRDQYLGRIERLRRVCPEIRMTSDVIVG 307
Query: 303 FPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSKKLIELN 362
FPG L+R ++ + F +S R GT A ++ + + K + +++ L
Sbjct: 308 FPGETESEFEQTMDLLERARFTEIYSFIFSARPGTSAADLPDDIPKEVKQQWFDRMLALQ 367
Query: 363 RINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSGKILNVKI-- 420
+ + + IG+V+ VL E G+G G T VN + ++ G+I+ V++
Sbjct: 368 EEITRQYHQMDIGQVLPVLVEGSSRQGNGQLFGRTTWNRIVNFDGNPDLVGRIVPVRLTV 427
Query: 421 ---NSFEGE 426
NS GE
Sbjct: 428 AYRNSHLGE 436
>MIAB_PROM2 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 464
Score = 196 bits (499), Expect = 2e-52
Identities = 134/431 (31%), Positives = 208/431 (48%), Gaps = 20/431 (4%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIGR-ARR 65
T GC++N +SE M + GY D AD+ + NTC++ + ++K +GR A+R
Sbjct: 25 TFGCQMNKADSERMAGTLEKMGYTRADDELKADLVLYNTCTIRDNAEQKVYSFLGRQAKR 84
Query: 66 QN--PEAIIAVVGCYAQI-APTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVKVG 122
++ P + V GC AQ + + + +D+V+G ++ + + K D G Q V
Sbjct: 85 KHKIPSLKLVVAGCLAQQEGESLLRRVPELDLVMGPQHVNNLENLLGKV-DLGNQ---VA 140
Query: 123 AVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDEIRS 182
A + + ED+ + ++ I GCN C+YC++P RG S+ P + EI+
Sbjct: 141 ATEETFISEDITNARRESSICGWVNIIYGCNERCSYCVVPSVRGKEQSRYPDAIKSEIQK 200
Query: 183 LAEHGFKEIILSGIHTASYGVDLD-------EKVTLVDLLEEIEKIDGIERVRIGSIDPT 235
LA FKEI L G + +YG DL ++ TL DLL I ++GI R+R + P
Sbjct: 201 LANDNFKEITLLGQNIDAYGRDLPGTTKEGRKENTLTDLLYYIHDVEGIRRIRFATSHPR 260
Query: 236 FFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSI 295
+F+ +++ L K+C HFH+ QSG + LK M R YT +Y+ I+ +R + + SI
Sbjct: 261 YFSRRLIQACYELDKVCEHFHIPFQSGNNEILKLMARGYTIDKYKRIIENIRSLMPNASI 320
Query: 296 TTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERS 355
T D IV FPG + I + YSPR T A NQ+ K ER
Sbjct: 321 TADAIVAFPGESEEQYRDTLKLISDIGFDQVMTAAYSPRPNTPAALWHNQISEEVKKERL 380
Query: 356 KKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTR----NYIKVNAKADCNV 411
K++ EL K E+Y+ + VL E S G TR ++++ N
Sbjct: 381 KEINELVETTSKQRNERYLDSIESVLIEGFNPKNSSQIMGRTRTNRLTFVEIPKNIKFNF 440
Query: 412 S-GKILNVKIN 421
S G ++VKIN
Sbjct: 441 SLGDEIDVKIN 451
>MIAB_PROAC RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 494
Score = 196 bits (499), Expect = 2e-52
Identities = 141/445 (31%), Positives = 214/445 (48%), Gaps = 22/445 (4%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEV---VDFNEMADVYVVNTCSVTNMGDKKSRQIIGR- 62
T GC++N ++SE + GY D ADV V NTC+V D + +G
Sbjct: 18 TYGCQMNVHDSERIAGLLEEAGYVPDPRTDTLAPADVVVFNTCAVRENADNRLYGTLGHM 77
Query: 63 --ARRQNPEAIIAVVGCYAQI-APTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQV 119
+ +P +AV GC AQ T V+ VDVV G+ N G + + +AR QV
Sbjct: 78 AAVKSAHPGMQLAVGGCMAQKDKDTVVARAPWVDVVFGTHNVGSLPVLLKRARHNATAQV 137
Query: 120 KVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDE 179
++ L F ++ A++ I GCN C +C++P RG + P ++L E
Sbjct: 138 EIEESLVT--FPSNLPARRDSAYSAWVSISVGCNNTCTFCIVPQLRGKETDRRPGEILSE 195
Query: 180 IRSLAEHGFKEIILSGIHTASYGVDLDEKVTLVDLLEEIEKIDGIERVRIGSIDPTFFTE 239
IR+L G +EI L G + SYGV ++ LL IDG+ERVR S P FT+
Sbjct: 196 IRALVNEGVQEITLLGQNVNSYGVQFGDRGAFAKLLRACGNIDGLERVRFTSPHPAAFTD 255
Query: 240 DVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITTDV 299
DV+ + + P H+ LQSG D L+RM R Y + ++ I++ +R+ I +ITTD+
Sbjct: 256 DVIEAMAETPNVMPSLHMPLQSGSDEVLRRMRRSYRSTKFLGILDRVREAIPHAAITTDI 315
Query: 300 IVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSKKLI 359
IVGFPG ++ S F+YS R T A M++QV ER ++L+
Sbjct: 316 IVGFPGETDKDFAETMRIVEESRFSAAFTFQYSIRPNTPAGVMKDQVPKPVVQERYEQLV 375
Query: 360 ELNRINEKAFAEK--YIGEVIDVLF---EEEVELGSGVYTGYTRN----YIKVNAKADCN 410
EL +++ A+ E +G ++V+F E + + TG R+ ++ +N D
Sbjct: 376 EL--VDDIAWQENKAQVGRAVEVMFAQGEGRKDEATHRVTGRARDNRLVHVSLNPGDDVP 433
Query: 411 VSGKILNVKINSFEGE--VAEGEIV 433
G I V I S VA+G +V
Sbjct: 434 RPGDIGEVVITSGHPHHLVADGGLV 458
>MIAB_HERAR RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 446
Score = 196 bits (499), Expect = 2e-52
Identities = 136/443 (30%), Positives = 215/443 (48%), Gaps = 12/443 (2%)
Query: 2 KVAFATLGCRVNTYESEAMTEKF-VREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S+ M + +G D E ADV ++NTCSV +K +
Sbjct: 4 KIYIKTFGCQMNEYDSDKMADVLNASDGLIKTDKAEDADVILLNTCSVREKAQEKVFSDL 63
Query: 61 GRARRQN---PEAIIAVVGCYA-QIAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR R P+ +I V GC A Q + VD+V G + + + + R G+
Sbjct: 64 GRLRELKLLKPDLMIGVGGCVASQEGDAIIKRAPYVDLVFGPQTLHRLPEMLKQRRSTGR 123
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
QV + K F+ + + T AF+ I +GC+++C+YC++PYTRG S+ V
Sbjct: 124 SQVDISFPEIEK-FDHMPPAKVEGAT-AFVSIMEGCSKYCSYCVVPYTRGEEVSRRFDDV 181
Query: 177 LDEIRSLAEHGFKEIILSGIHTASYGVDL--DEKVTLVDLLEEIEKIDGIERVRIGSIDP 234
L E+ L G KEI L G + +Y ++ E L+E I +++GIER+R + P
Sbjct: 182 LTEVAGLEAQGVKEITLLGQNVNAYRGEMADGEIADFALLIEYIAEMEGIERIRYVTSHP 241
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
FT+ ++ + KL H +L Q G D L M R YT+ EY+ I+ LR ++S
Sbjct: 242 KEFTQRLIDTYAKVPKLVDHLYLPAQHGSDRILAAMKRGYTSLEYKSILRRLRAVRPNIS 301
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
I++D IVGFPG + I + F +SPR GT A N+ + K +R
Sbjct: 302 ISSDFIVGFPGETDEDFEAMMKLIDDIGYDNSFSFIFSPRPGTPAANLADDTPHEVKLKR 361
Query: 355 SKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVS-- 412
++L + N + ++++ +G V +L E + + G T N VN A N +
Sbjct: 362 LQRLQAVIDQNTRRYSDEMVGSVQRILVEGPSKKDADELQGRTENNRVVNFSAGPNAAHL 421
Query: 413 -GKILNVKINSFEGEVAEGEIVL 434
G+++NV I GEIV+
Sbjct: 422 IGQMVNVNITQSHAYTLRGEIVV 444
>MIAB_DELAS RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 442
Score = 196 bits (499), Expect = 2e-52
Identities = 134/439 (30%), Positives = 210/439 (47%), Gaps = 8/439 (1%)
Query: 2 KVAFATLGCRVNTYESEAMTEKF-VREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
KV T GC++N Y+S+ M + +GYE D E AD+ + NTCSV +K +
Sbjct: 4 KVFIKTFGCQMNEYDSDKMADVLGAAQGYESTDDPEQADLILFNTCSVREKAQEKVFSDL 63
Query: 61 GRARRQNPEAI-IAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQ 118
GR + + I V GC A E+ VDVV G + + +N + K Q
Sbjct: 64 GRYKHLKERGVLIGVGGCVASQEGEEIIKRAPYVDVVFGPQTLHRLPELLNARAAKHKPQ 123
Query: 119 VKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLD 178
V + K F+ L + AF+ I +GC+++C+YC++PYTRG S+ + VL
Sbjct: 124 VDISFPEIEK-FDHLPPARVEGSS-AFVSIMEGCSKYCSYCVVPYTRGEEVSRPFEDVLI 181
Query: 179 EIRSLAEHGFKEIILSGIHTASYGVDLDEKVTLVD---LLEEIEKIDGIERVRIGSIDPT 235
E+ LA+ G KE+ L G + +Y + + + D LLE + +I GIER+R + P
Sbjct: 182 EVAGLADQGVKEVTLLGQNVNAYLGKMGDTAEIADFALLLEYVSEIPGIERIRYTTSHPN 241
Query: 236 FFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSI 295
FT ++ L KL H HL +Q G D L M R YTA EY+ + LR ++++
Sbjct: 242 EFTPRLIEAYARLPKLVSHLHLPVQHGSDKILMAMKRGYTAMEYKSTIRKLRAIRPEMAM 301
Query: 296 TTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERS 355
++D IVGFPG + + + F +SPR GT A N+ + K R
Sbjct: 302 SSDFIVGFPGETEEDFQKMMKLIHDVRFDNSFSFIFSPRPGTPAANLHDDTPHEVKLRRL 361
Query: 356 KKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSGKI 415
++L + N K +++ +G V +L E + G T VN + + G++
Sbjct: 362 QELQAVINNNIKDISDERVGTVQRLLVEGLSKRDGSELMGRTECNRVVNFPGNERLIGQM 421
Query: 416 LNVKINSFEGEVAEGEIVL 434
++VKI GE+V+
Sbjct: 422 IDVKITEARTFTLRGEVVV 440
>MIAB_ACISJ RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 448
Score = 196 bits (499), Expect = 2e-52
Identities = 135/439 (30%), Positives = 209/439 (47%), Gaps = 8/439 (1%)
Query: 2 KVAFATLGCRVNTYESEAMTEKF-VREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
KV T GC++N Y+S+ M + +GYE D E AD+ + NTCSV +K +
Sbjct: 4 KVFIKTFGCQMNEYDSDKMADVLGAAQGYEPTDDPEQADLILFNTCSVREKAQEKVFSDL 63
Query: 61 GRARRQNPEAI-IAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQ 118
GR + + + I V GC A E+ VDVV G + + +N + + Q
Sbjct: 64 GRVKHLKDKGVLIGVGGCVASQEGEEIIKRAPFVDVVFGPQTLHRLPELLNARAAKARPQ 123
Query: 119 VKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLD 178
V + K F+ L + AF+ I +GC+++C+YC++PYTRG S+ + VL
Sbjct: 124 VDISFPEIEK-FDHLPPARVEGAS-AFVSIMEGCSKYCSYCVVPYTRGEEVSRPFEDVLV 181
Query: 179 EIRSLAEHGFKEIILSGIHTASY-GV--DLDEKVTLVDLLEEIEKIDGIERVRIGSIDPT 235
E+ LA+ G KE+ L G + +Y G D EK LLE + +I GIER+R + P
Sbjct: 182 EVAGLADQGVKEVTLLGQNVNAYLGAMGDTAEKADFALLLEYVAEIPGIERIRFTTSHPN 241
Query: 236 FFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSI 295
FT ++ + KL H HL +Q G D L M R YTA EY+ + LR D+++
Sbjct: 242 EFTPRLIEAYAKIPKLVSHLHLPVQHGSDRILMAMKRGYTAMEYKSTIRKLRAIRPDMAM 301
Query: 296 TTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERS 355
++D IVGFPG + + + F +SPR GT A N+ + K R
Sbjct: 302 SSDFIVGFPGETEEDFQKMMKLIDDVRFDNSFSFIFSPRPGTPAANLHDDTPHEVKLRRL 361
Query: 356 KKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSGKI 415
++L + N +++ +G V +L E + G T VN + G++
Sbjct: 362 QELQAVINRNILEISQERVGTVQRLLVEGVSKRDGSELMGRTECNRVVNFPGHERLIGQM 421
Query: 416 LNVKINSFEGEVAEGEIVL 434
++VKI GE+V+
Sbjct: 422 IDVKITEARTYTLRGEVVM 440
>MIAB_SULDN RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 435
Score = 196 bits (498), Expect = 3e-52
Identities = 140/442 (31%), Positives = 211/442 (47%), Gaps = 23/442 (5%)
Query: 2 KVAFATLGCRVNTYESEAM-TEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ TLGC +N+ +SE M E +EGYE + AD+ ++NTCSV K +
Sbjct: 6 KLFIETLGCAMNSRDSEHMIAELSEKEGYETTNDLSSADLIIINTCSVREKPVAKLFSEL 65
Query: 61 GRARRQNPE-AIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQ 118
G ++ E A I V GC A E+ V VLG+RN +I ++K R +
Sbjct: 66 GVFNKRKKEGAKIGVCGCTASHLGEEIIKRAPYVSFVLGARNVSKISEVLHKER---AVE 122
Query: 119 VKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLD 178
+ + F D + Y +A++ I GC++ C YC++P TRG S + +L
Sbjct: 123 IDINYDESEFAFNDFRTSPY----KAYINISIGCDKSCTYCIVPKTRGEEISIPTELILR 178
Query: 179 EIRSLAEHGFKEIILSGIHTASYGVDLD---EKVTLVDLLEEIEKIDGIERVRIGSIDPT 235
E + + G KEI L G + +YG EKV+ +LL + KID +ER+R S P
Sbjct: 179 EAQKAVKGGAKEIFLLGQNVNNYGRRFSGEHEKVSFSELLRRLSKIDALERIRFTSPHPF 238
Query: 236 FFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSI 295
++ + + K+C H+ LQSG LK M R YT + + + V LR DVSI
Sbjct: 239 HMDDEFIEEFSSNPKICKSMHMPLQSGSSKVLKDMKRGYTKEWFLNRVQKLRAMSPDVSI 298
Query: 296 TTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERS 355
+TD+IV FPG ++ + + FKYSPR T AE+ N VD +D S
Sbjct: 299 STDIIVAFPGESDEDFEETLSIMREVKFDQIFSFKYSPRPETEAEHFTNIVD---EDVAS 355
Query: 356 KKLIELNRINEKAFAEK---YIGEVIDVLFEEEVELGSGVY-TGYTRNYIKVNAKADCNV 411
+L L + EK ++G++ V FE +L Y +G + N + + K +
Sbjct: 356 SRLTTLQNLAVNILDEKNKTHLGKIYRVYFE---DLNQDYYASGRSDNNLLIKVKGSDEL 412
Query: 412 SGKILNVKINSFEGEVAEGEIV 433
G+ +VKI + GEIV
Sbjct: 413 LGEFRDVKITDIGRTILSGEIV 434
>MIAB_POLSQ RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 448
Score = 196 bits (498), Expect = 3e-52
Identities = 133/443 (30%), Positives = 215/443 (48%), Gaps = 13/443 (2%)
Query: 2 KVAFATLGCRVNTYESEAMTEKF-VREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S M + EG + D E ADV ++NTCS+ + K +
Sbjct: 3 KLYIKTFGCQMNEYDSGKMADLLHADEGMVMTDTPEDADVVLLNTCSIREKAEDKVFSDL 62
Query: 61 GRAR---RQNPEAIIAVVGCYA-QIAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR R + P +I V GC A Q VS VDVV G + + + + R G
Sbjct: 63 GRLRELKKTKPHMLIGVGGCVASQEGRQIVSRAPYVDVVFGPQTLHRLSDLIAQRRKTGL 122
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
QV + K F+ L + A++ I +GC+++C+YC++PYTRG S+ V
Sbjct: 123 SQVDISFPEIEK-FDHLPASR-QTRGSAYVSIMEGCSKYCSYCVVPYTRGEEVSRPFDDV 180
Query: 177 LDEIRSLAEHGFKEIILSGIHTASYGVDLDEKVTLVD---LLEEIEKIDGIERVRIGSID 233
L E+ LA G KEI+L G + +Y + + + D L+E I +I G+ER+R +
Sbjct: 181 LTEVAGLASKGVKEIVLLGQNVNAYLGKMGDAEEIADFALLIEYIAEIPGVERIRFTTSH 240
Query: 234 PTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDV 293
P FT+ ++ + KL H HL +Q G D+ L M R YTA EY+ I+ +R D+
Sbjct: 241 PKEFTQRLIDVYAKVPKLVSHLHLPVQHGSDSILSAMKRGYTALEYKSIIRKMRAVRPDL 300
Query: 294 SITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDE 353
++++D IVGFPG ++ + + F +SPR GT A N+ + K +
Sbjct: 301 TLSSDFIVGFPGETDADFEKLLKMVQELDFDNSFCFIFSPRPGTPAANLSDDTPYEVKLK 360
Query: 354 RSKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVS- 412
R + L+ L ++K +G+ VL E + G + N + + D N+
Sbjct: 361 RLQTLLALIEGQSNQISQKMLGKTERVLIEGLAKDGVNLQGRAENNRVIHFSPPDQNIDG 420
Query: 413 --GKILNVKINSFEGEVAEGEIV 433
G++++++I GE+V
Sbjct: 421 LIGEMVDIRITEVLNYTLRGELV 443
>MIAB_JANSC RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 462
Score = 196 bits (498), Expect = 3e-52
Identities = 129/437 (29%), Positives = 212/437 (48%), Gaps = 8/437 (1%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIG 61
K+ T GC++N Y+SE M E EGYE V+ E AD+ ++NTC + +K +G
Sbjct: 29 KLFVKTYGCQMNVYDSERMAEALGGEGYEQVNTPEGADMILLNTCHIREKAAEKMYSELG 88
Query: 62 RARR---QNPEAIIAVVGCYAQIAPTEVSGIEG-VDVVLGSRNKGEIVYYVNKARDEGKQ 117
R R NP+ I V GC AQ E+ + VD+V+G + + + +A + G++
Sbjct: 89 RLRPLRDANPDLKIGVAGCVAQAEGEEIMRRQPLVDLVVGPQTYHRLPKMM-EAVNAGEK 147
Query: 118 QVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVL 177
+ ++K K T AFL +Q+GC++FCA+C++PYTRG+ S+ ++++
Sbjct: 148 ALDTDFPEEDKFLNLPKARATRGPT-AFLTVQEGCDKFCAFCVVPYTRGAEVSRSAERLM 206
Query: 178 DEIRSLAEHGFKEIILSGIHTASY-GVDLDEKVTLVDLLEEIEKIDGIERVRIGSIDPTF 236
E R L + G +EI L G + +Y G L L+ E+ KIDG++R+R + P
Sbjct: 207 AEARDLVDRGVREITLLGQNVNAYHGAGDGGTWGLARLIREMAKIDGLDRIRFTTSHPND 266
Query: 237 FTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSIT 296
+D++ +L P+ HL +Q+G D LK MNR++TA +Y +++ +RD D+ ++
Sbjct: 267 MEDDLIAAHGDCPELMPYLHLPVQAGSDKILKAMNRKHTAADYIRLIDRIRDARPDLHLS 326
Query: 297 TDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSK 356
D IVGFPG ++ + + FKYS R GT A QV + +
Sbjct: 327 GDFIVGFPGETEEDFQATLDLVETVGYGTAYSFKYSARPGTPAAERTGQVSEAEASDXXX 386
Query: 357 KLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSGKIL 416
+ +G + VLFE+ G G + V+ + G+I
Sbjct: 387 XXXXXXXXXXXXXXDAMVGRRVKVLFEKPGR-NPGQMIGKSEYLHSVHVDGPDTLRGQIA 445
Query: 417 NVKINSFEGEVAEGEIV 433
V+I + G +V
Sbjct: 446 EVEIAESKTNSLTGRLV 462
>MIAB_RICPR RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 445
Score = 196 bits (497), Expect = 4e-52
Identities = 133/444 (29%), Positives = 228/444 (51%), Gaps = 21/444 (4%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIG 61
K+ T GC++N Y+S + + GYE + + AD+ ++NTC + +K+ +G
Sbjct: 4 KLYIKTYGCQMNVYDSVKIQDLLYPFGYESTEDIKEADIIILNTCHIREKAAEKTYSELG 63
Query: 62 RARR-QNPE-------AIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKAR 112
R ++ QN AII V GC AQ E+ S VD+V+G ++ + ++K
Sbjct: 64 RIKKLQNTRKQEGLNPAIIVVAGCVAQAEGEEIFSRTPYVDIVVGPQSYYNLPELISKVV 123
Query: 113 DEGKQQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKD 172
KQ + + V + K F++L + Y +F+ +Q+GC++FC +C++PYTRG+ S+
Sbjct: 124 RHEKQLIDLDFVEEAK-FDNLPEQLYPQGASSFISVQEGCDKFCTFCVVPYTRGAEFSRS 182
Query: 173 PKKVLDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKV-TLVDLLEEIEKIDGIERVRIG 230
++V E + KEIIL G + +Y G +K+ TL DLL+ + +I + R+R
Sbjct: 183 VEQVYRESLKAVSNDAKEIILLGQNVNAYHGKGPKDKIFTLADLLKCLAQIPNLARLRYM 242
Query: 231 SIDPTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKI 290
+ P T+D+++ KL P HL +QSG + LK MNR++ Y +I+N LR+
Sbjct: 243 TSHPIDMTDDLIKLHGTEPKLMPFLHLPVQSGSNKILKAMNRKHDRDYYFNIINRLREAR 302
Query: 291 EDVSITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNK 350
D+ +++D IVGFPG + R+ + + FKYSPR GT +Q+ +
Sbjct: 303 SDIVLSSDFIVGFPGETEQDFEDTLDLVHRVKYGQCYSFKYSPRPGTPGAIRTDQIPEHI 362
Query: 351 KDERSKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVY--TGYTRNYIKVNAKAD 408
K +R L + + AF + +G + VLF+ + + + T Y ++ N
Sbjct: 363 KSKRLTILQQELATQQLAFNQSCVGSTMRVLFDRDGKFEDQIIGKTPYMQSVYIHNPNK- 421
Query: 409 CNVSGKILNV-----KINSFEGEV 427
++ GKI++V +NS GE+
Sbjct: 422 -SLLGKIVDVIITKAALNSLTGEI 444
>MIAB_NEIMA RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 442
Score = 196 bits (497), Expect = 4e-52
Identities = 140/441 (31%), Positives = 206/441 (46%), Gaps = 15/441 (3%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVRE--GYEVVDFNEMADVYVVNTCSVTNMGDKKSRQI 59
KV T GC++N Y+S+ M E G E V + AD+ + NTCSV +K
Sbjct: 3 KVFIRTFGCQMNEYDSDKMLAVLAEEHGGIEQVTQADEADIILFNTCSVREKAQEKVFSD 62
Query: 60 IGRAR---RQNPEAIIAVVGCYA-QIAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEG 115
+GR R + P II V GC A Q + VDVV G + + + G
Sbjct: 63 LGRVRPLKEKKPGLIIGVAGCVASQEGENIIKRAPYVDVVFGPQTLHRLPKMIVDKETSG 122
Query: 116 KQQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKK 175
QV + K F+ L AF+ I +GC+++C++C++PYTRG S+
Sbjct: 123 LSQVDISFPEIEK-FDHLPPARVEGGA-AFVSIMEGCSKYCSFCVVPYTRGEEFSRPLND 180
Query: 176 VLDEIRSLAEHGFKEIILSGIHTASYGVDLD--EKVTLVDLLEEIEKIDGIERVRIGSID 233
VL EI +LA+ G KEI L G + +Y ++D E LL + +I GIER+R +
Sbjct: 181 VLTEIANLAQQGVKEINLLGQNVNAYRGEMDDGEICDFATLLRIVHEIPGIERMRFTTSH 240
Query: 234 PTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDV 293
P FT+ ++ L KL H HL +QSG D L M R YTA EY+ I+ LR D+
Sbjct: 241 PREFTDSIIECYRDLPKLVSHLHLPIQSGSDRVLSAMKRGYTALEYKSIIRKLRAIRPDL 300
Query: 294 SITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDE 353
+++D IVGFPG +K IA + +F YSPR GT A N+ + +K
Sbjct: 301 CLSSDFIVGFPGETEREFEQTLKLVKDIAFDLSFVFIYSPRPGTPAANLHDDTPHEEKVR 360
Query: 354 RSKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSG 413
R + L E+ + IG V L E + T N VN ++
Sbjct: 361 RLEALNEVIEAETARINQTMIGTVQRCLVEGISKKDPDQLQARTANNRVVNFTGTPDMIN 420
Query: 414 KILNVKIN-----SFEGEVAE 429
++++++I S G++ E
Sbjct: 421 QMIDLEITEAYTFSLRGKIVE 441
>MIAB_MYCA9 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 507
Score = 196 bits (497), Expect = 4e-52
Identities = 126/401 (31%), Positives = 193/401 (48%), Gaps = 16/401 (3%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIGRA--- 63
T GC++N ++SE ++ GY AD+ + NTC+V D K I
Sbjct: 19 TYGCQMNVHDSERVSGLLDAAGYVKAPEGTDADIVIFNTCAVRENADNKLYGNISHLAPR 78
Query: 64 RRQNPEAIIAVVGCYAQI-APTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVKVG 122
+ NP IAV GC AQ ++ VDVV G+ N G + + +AR + QV++
Sbjct: 79 KAANPNMQIAVGGCLAQKDREGMLAKAPWVDVVFGTHNIGSLPALLERARHNNEAQVEIV 138
Query: 123 AVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDEIRS 182
L++ F + A++ I GCN C +C++P RG + P +L E+++
Sbjct: 139 EALEH--FPSALPATRESAYAAWVSISVGCNNTCTFCIVPSLRGKEIDRRPGDILGEVQA 196
Query: 183 LAEHGFKEIILSGIHTASYGVDLDE------KVTLVDLLEEIEKIDGIERVRIGSIDPTF 236
L + G E+ L G + +YGV+ + + +LL +IDG+ERVR S P
Sbjct: 197 LVDQGVLEVTLLGQNVNAYGVNFADPEIPRDRGAFAELLRACGRIDGLERVRFTSPHPAE 256
Query: 237 FTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSIT 296
FT+DV+ + +CP H+ LQSG D LK M R Y ++ + I++ +R + D +IT
Sbjct: 257 FTDDVIEAMAQTPNVCPQLHMPLQSGSDRILKAMRRSYRSERFLSIIDKVRSAMPDAAIT 316
Query: 297 TDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSK 356
TD+IVGFPG ++R S F+YS R GT A + +Q+ ER
Sbjct: 317 TDIIVGFPGETEEDFQQTLEVVRRARFSSAFTFQYSIRPGTPAAKLPDQLPKAVVQERYD 376
Query: 357 KLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYT 397
+LI L +K IG +I+VL + G G G T
Sbjct: 377 RLIALQESVTLEENQKQIGRMIEVL----IATGEGRKDGET 413
>MIAB_KOCRD RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 513
Score = 196 bits (497), Expect = 4e-52
Identities = 125/379 (32%), Positives = 185/379 (48%), Gaps = 7/379 (1%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIGR---A 63
TLGC++N ++SE +T GY V D+ V NTC+V D K +G+
Sbjct: 33 TLGCQMNAHDSERITGLLENAGY-VPAVGREPDLVVFNTCAVRENADNKLYGQLGQLAPV 91
Query: 64 RRQNPEAIIAVVGCYAQI-APTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVKVG 122
+R+N IAV GC AQ T V VDVV G+ N G + + +AR + QV++
Sbjct: 92 KRRNDRLQIAVGGCLAQKDRSTIVEKAPWVDVVFGTHNIGSLPVLLERARHNQQAQVEIL 151
Query: 123 AVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDEIRS 182
L+ F + ++ I GCN C +C++P RG + P +L E+R+
Sbjct: 152 ESLET--FPSALPTKREQAYAGWVSISVGCNNTCTFCIVPQLRGKEKDRRPGDILAEVRA 209
Query: 183 LAEHGFKEIILSGIHTASYGVDLDEKVTLVDLLEEIEKIDGIERVRIGSIDPTFFTEDVV 242
L + G E+ L G + SYGV+ ++ LL +I+G+ER+R S P FT+DV+
Sbjct: 210 LVDSGAVEVTLLGQNVNSYGVEFGDRGAFAKLLRACGEIEGLERIRFTSPHPAAFTDDVI 269
Query: 243 RRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITTDVIVG 302
+ + P H+ LQSG D LKRM R Y + + I++ +RD+I D +ITTD+IVG
Sbjct: 270 DAMAETPNVMPQLHMPLQSGSDRILKRMRRSYRSTRFLGILDRVRDRIPDAAITTDIIVG 329
Query: 303 FPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSKKLIELN 362
FPG + S F+YS R GT A M +QV ER ++L L
Sbjct: 330 FPGETEQDFQDTLDVVAASEFSSAFTFQYSIRPGTPAGEMADQVPAAVVQERFERLTALQ 389
Query: 363 RINEKAFAEKYIGEVIDVL 381
+ +G +VL
Sbjct: 390 DGISARLNRQLVGTRAEVL 408
>MIAB_KLEP7 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 474
Score = 196 bits (497), Expect = 4e-52
Identities = 136/444 (30%), Positives = 216/444 (48%), Gaps = 18/444 (4%)
Query: 2 KVAFATLGCRVNTYESEAMTEKF-VREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S M + GY++ + E ADV ++NTCS+ +K +
Sbjct: 4 KLHIKTWGCQMNEYDSSKMADLLDATHGYQLTEVAEEADVLLLNTCSIREKAQEKVFHQL 63
Query: 61 GR---ARRQNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR + +NP+ II V GC A + VD++ G + + +N R
Sbjct: 64 GRWKLLKEKNPDLIIGVGGCVASQEGDHIRQRAHYVDIIFGPQTLHRLPEMINSVRGNRS 123
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
V + K F+ L T AF+ I +GCN++C YC++PYTRG S+ +
Sbjct: 124 PVVDISFPEIEK-FDRLPEPRAEGPT-AFVSIMEGCNKYCTYCVVPYTRGEEVSRPCDDI 181
Query: 177 LDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKV-TLVDLLEEIEKIDGIERVRIGSIDP 234
L EI LA G +E+ L G + ++ G + D + DLL + IDGI+R+R + P
Sbjct: 182 LFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSFADLLRLVAAIDGIDRIRFTTSHP 241
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
FT+D++ +L HL +QSG D L M R +TA EY+ I+ LR+ D+
Sbjct: 242 IEFTDDIIDVYRDTPELVSFLHLPVQSGSDRVLNLMGRTHTALEYKAIIRKLREARPDIQ 301
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
I++D IVGFPG + + ++ F +S R GT A +M + V + D++
Sbjct: 302 ISSDFIVGFPGETTEDFEKTMKLIADVNFDMSYSFIFSARPGTPAADMVDDVP--EADKK 359
Query: 355 SKKLIELNRINEKAFA--EKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVS 412
+ I RIN++A A + +G V +L E +G T N VN + ++
Sbjct: 360 QRLYILQERINQQAMAWSRRMLGTVQRILVEGTSRKNIMELSGRTENNRVVNFEGTPDLV 419
Query: 413 GKILNVKI-----NSFEGEVAEGE 431
GK ++V+I NS G++ E
Sbjct: 420 GKFVDVEIVDVYTNSLRGKIVRTE 443
>MIAB_ENT38 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 474
Score = 196 bits (497), Expect = 4e-52
Identities = 138/444 (31%), Positives = 217/444 (48%), Gaps = 18/444 (4%)
Query: 2 KVAFATLGCRVNTYESEAMTEKF-VREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S M + GYE+ + E ADV ++NTCS+ +K ++
Sbjct: 4 KLHIKTWGCQMNEYDSSKMADLLNATHGYELTELAEEADVLLLNTCSIREKAQEKVFHLL 63
Query: 61 GR---ARRQNPEAIIAVVGCYA-QIAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR +++NP+ II V GC A Q + VD+V G + + +N R +
Sbjct: 64 GRWKLLKKKNPDLIIGVGGCVASQEGKLIRTRSPYVDIVFGPQTLHRLPEMINSVRGDRS 123
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
V V K D E + AF+ I +GCN++C YC++PYTRG S+ +
Sbjct: 124 PVVDVSFPEIEKF--DRLPEPRADGPSAFVSIMEGCNKYCTYCVVPYTRGEEVSRPCDDI 181
Query: 177 LDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKV-TLVDLLEEIEKIDGIERVRIGSIDP 234
L E+ LA G +E+ L G + ++ G + D + T +LL + IDGI+RVR + P
Sbjct: 182 LFEVAQLAAQGVREVNLLGQNVNAWRGENYDGSIGTFAELLRLVAAIDGIDRVRFTTSHP 241
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
FT+D++ +L HL +Q G D L M R +T EY+ I+ LR+ D+
Sbjct: 242 IEFTDDIIDVYRDTPELVSFLHLPVQCGSDRVLNLMGRTHTVLEYKAIIRKLREARPDIQ 301
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
I++D IVGFPG + I + F +S R GT A +M + V +K +R
Sbjct: 302 ISSDFIVGFPGETTDDFERTMKLIGEINFDVSFSFIFSARPGTPAADMVDDVPEEEKKQR 361
Query: 355 SKKLIELNRINEK--AFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVS 412
L E RIN++ A++ + +G +L E +G T N VN + ++
Sbjct: 362 LYILQE--RINQQVTAWSRRMLGTTQRILVEGTSRKSVMQLSGRTENNRVVNFEGTPDMI 419
Query: 413 GKILNVKI-----NSFEGEVAEGE 431
GK ++++I NS G+V E
Sbjct: 420 GKFVDIEILEVLTNSMRGKVVRTE 443
>MIAB_ANAMM RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 455
Score = 196 bits (497), Expect = 4e-52
Identities = 124/379 (32%), Positives = 197/379 (51%), Gaps = 10/379 (2%)
Query: 9 GCRVNTYESEAMTEKFVRE-GYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIGRARRQN 67
GC++N Y+S M E +R G+ V E AD+ +VNTC V +K +GR R
Sbjct: 11 GCQMNVYDS-LMVEDILRPLGFAAVQRPEDADIIMVNTCHVREKAAEKLYSALGRMRMLR 69
Query: 68 PE-AIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQ-VKVGAV 124
E A+I V GC AQ V VDVV+G ++ + + KA + KQ V+ A+
Sbjct: 70 KEGALIVVAGCVAQAEGEAVFERAPFVDVVVGPQSIHTLPELIMKATRDAKQMNVEFPAI 129
Query: 125 LKNKVFE-DLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDEIRSL 183
K V DL + + AF+ +Q+GC++FC +C++PYTRG S+ + VL E+R L
Sbjct: 130 SKFDVISTDLLV---SRGVSAFVSVQEGCDKFCTFCVVPYTRGPEYSRSVEDVLAEVRKL 186
Query: 184 AEHGFKEIILSGIHTASY-GVDLDEKVTLVDLLEEIEKIDGIERVRIGSIDPTFFTEDVV 242
A+ G KE++L G + +Y G + L L+ ++ +++GIER+R + P +
Sbjct: 187 ADGGTKEVVLLGQNVNAYHGTYKGNEWDLGRLIRKVAEVEGIERIRYTTSHPRDMHSSLY 246
Query: 243 RRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITTDVIVG 302
KL P HL +QSG D+ L++MNR+++ +EY DI++ L++ D+++++D IVG
Sbjct: 247 DAHKHEPKLLPCVHLPVQSGSDSVLRKMNRKHSVQEYMDIIDTLKEARSDIALSSDFIVG 306
Query: 303 FPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSKKLIELN 362
FPG ++ + + ++ FKYSPR GT NQV +K
Sbjct: 307 FPGETEEDFEATMDLVRHVGFATSYSFKYSPRPGTPGAEYTNQVPEEEKSSXXXXXXXXX 366
Query: 363 RINEKAFAEKYIGEVIDVL 381
F + IG+ + VL
Sbjct: 367 XXXXXXFTKSMIGKTVSVL 385
>MIAB_SHIB3 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 474
Score = 195 bits (496), Expect = 5e-52
Identities = 138/444 (31%), Positives = 214/444 (48%), Gaps = 18/444 (4%)
Query: 2 KVAFATLGCRVNTYESEAMTEKF-VREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S M + Y++ D E ADV ++NTCS+ +K +
Sbjct: 4 KLHIKTWGCQMNEYDSSKMADLLDATHSYQLTDVAEEADVLLLNTCSIREKAQEKVFHQL 63
Query: 61 GR---ARRQNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR + +NP+ II V GC A + VD++ G + + +N R +
Sbjct: 64 GRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDIIFGPQTLHRLPEMINSVRGDRS 123
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
V + K F+ L T AF+ I +GCN++C YC++PYTRG S+ +
Sbjct: 124 PVVDISFPEIEK-FDRLPEPRAEGPT-AFVSIMEGCNKYCTYCVVPYTRGEEVSRPSDDI 181
Query: 177 LDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKV-TLVDLLEEIEKIDGIERVRIGSIDP 234
L EI LA G +E+ L G + ++ G + D + DLL + IDGI+R+R + P
Sbjct: 182 LFEIAQLAAQGVREVNLLGQNVNAWRGENYDGSTGSFADLLRLVAAIDGIDRIRFTTSHP 241
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
FT+D++ +L HL +QSG D L M R +TA EY+ I+ LR D+
Sbjct: 242 IEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNLMGRTHTALEYKAIIRKLRAARPDIQ 301
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
I++D IVGFPG + + ++ F +S R GT A +M + V +K +R
Sbjct: 302 ISSDFIVGFPGETTEDFEKTMKLIADVNFDMSYSFIFSARPGTPAADMVDDVPEEEKKQR 361
Query: 355 SKKLIELNRINEKAFA--EKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVS 412
L E RIN++A A + +G +L E +G T N VN + ++
Sbjct: 362 LYILQE--RINQQAMAWSRRMLGTTQRILVEGTSRKSIMELSGRTENNRVVNFEGTPDMI 419
Query: 413 GKILNVKI-----NSFEGEVAEGE 431
GK ++V+I NS G+V E
Sbjct: 420 GKFVDVEITDVYPNSLRGKVVRTE 443
>MIAB_AGRT5 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 455
Score = 195 bits (496), Expect = 5e-52
Identities = 133/446 (29%), Positives = 220/446 (49%), Gaps = 22/446 (4%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIG 61
KV T GC++N Y+S M++ ++GY + AD+ ++NTC + +K +G
Sbjct: 11 KVFIKTYGCQMNVYDSVRMSDALAKDGYVQTEDMGEADLVLLNTCHIREKAAEKVYSALG 70
Query: 62 RAR-------RQNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARD 113
R R Q E +I V GC AQ E+ VDVV+G + + + + R
Sbjct: 71 RLRDMKKSREEQGREFMIGVAGCVAQAEGEEILRRAPAVDVVIGPQTYHRLPDALKRVR- 129
Query: 114 EGKQQVKVGAVLKNKVFEDLKIEEY----NNKTRAFLKIQDGCNRFCAYCLIPYTRGSVC 169
G++ ++ +++K FE L + E + AFL +Q+GC++FC +C++PYTRGS
Sbjct: 130 RGERVIETEYAVEDK-FEHLPVAEKATLRSRGVTAFLTVQEGCDKFCTFCVVPYTRGSEV 188
Query: 170 SKDPKKVLDEIRSLAEHGFKEIILSGIHTASYGVD--LDEKVTLVDLLEEIEKIDGIERV 227
S+ ++++DE L + G +EI L G + ++ + EK L +LL + +I G+ R+
Sbjct: 189 SRPVRQIVDEAMKLVDAGVREITLLGQNVNAWQGEGPKGEKWGLAELLYRLAEIPGLARL 248
Query: 228 RIGSIDPTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLR 287
R + P + ++ L+ L P+ HL +QSG D LK MNRR+T +EY ++ +R
Sbjct: 249 RYTTSHPRDMDDRLIGAHRDLRILMPYLHLPVQSGSDRILKAMNRRHTGEEYIQLIEKIR 308
Query: 288 DKIEDVSITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVD 347
D++++ D IVGFPG ++ + ++ FKYS R GT ++ +QV
Sbjct: 309 SARPDIAMSGDFIVGFPGETDRDFEDTMAMVETVKYAQAFSFKYSTRPGTPGADLTDQVA 368
Query: 348 GNKKDERSKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKA 407
+ FAE +G+ +DVL E+ + + G + VN A
Sbjct: 369 EDVXXXXXXXXXXXXXXXXXXFAESLVGKTMDVLLEKPGRMPEQL-IGRSPWLQSVNLDA 427
Query: 408 DCNVSGKILNVKI-----NSFEGEVA 428
G I+NV+I NS EVA
Sbjct: 428 KTLKIGDIVNVRITATGPNSLFAEVA 453
>MIAB_YERPN RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 474
Score = 195 bits (495), Expect = 7e-52
Identities = 134/428 (31%), Positives = 210/428 (49%), Gaps = 13/428 (3%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVR-EGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S M + GY++ E AD+ ++NTCS+ +K ++
Sbjct: 4 KLHIKTWGCQMNEYDSSKMADLLASTHGYQLTTIPEEADLLLLNTCSIREKAQEKVFSLL 63
Query: 61 GR---ARRQNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
G+ + +NP+ II V GC A ++ VDV+ G + + +N +
Sbjct: 64 GQWKLLKEKNPQLIIGVGGCVASQEGEQLRQRAPCVDVIFGPQTLHRLPEMINHVQGTNS 123
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
V + K F+ L T AF+ I +GCN++C +C++PYTRG S+ +
Sbjct: 124 PVVDISFPEIEK-FDRLPEPRAEGPT-AFVSIMEGCNKYCTFCVVPYTRGEEVSRPSDDI 181
Query: 177 LDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKV-TLVDLLEEIEKIDGIERVRIGSIDP 234
L EI LA G +E+ L G + +Y G D + + +LL + IDGI+RVR + P
Sbjct: 182 LFEIAQLAAQGVREVNLLGQNVNAYRGATYDGDICSFAELLRLVAAIDGIDRVRFTTSHP 241
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
FT+D++ +L HL +QSG D L M R +TA EY+ I+ LR D+
Sbjct: 242 IEFTDDIIDVYRDTPELVSFLHLPVQSGSDRILTMMKRAHTALEYKAIIRKLRQARPDIQ 301
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
I++D IVGFPG + I ++ F YSPR GT A ++ N V +K +R
Sbjct: 302 ISSDFIVGFPGETQQDFEQTMKLVADIHFDTSYSFIYSPRPGTPAADLPNNVSEEEKKQR 361
Query: 355 SKKLIELNRINEKA--FAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVS 412
L + RI+++A + K +G V VL E G T N VN + ++
Sbjct: 362 LHILQQ--RISQQAMEISRKMVGTVQRVLVEGTSRKNVMELAGRTENNRVVNFEGSPDMI 419
Query: 413 GKILNVKI 420
GK ++V+I
Sbjct: 420 GKFVDVEI 427
>MIAB_YERPE RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 474
Score = 195 bits (495), Expect = 7e-52
Identities = 134/428 (31%), Positives = 210/428 (49%), Gaps = 13/428 (3%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVR-EGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S M + GY++ E AD+ ++NTCS+ +K ++
Sbjct: 4 KLHIKTWGCQMNEYDSSKMADLLASTHGYQLTTIPEEADLLLLNTCSIREKAQEKVFSLL 63
Query: 61 GR---ARRQNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
G+ + +NP+ II V GC A ++ VDV+ G + + +N +
Sbjct: 64 GQWKLLKEKNPQLIIGVGGCVASQEGEQLRQRAPCVDVIFGPQTLHRLPEMINHVQGTNS 123
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
V + K F+ L T AF+ I +GCN++C +C++PYTRG S+ +
Sbjct: 124 PVVDISFPEIEK-FDRLPEPRAEGPT-AFVSIMEGCNKYCTFCVVPYTRGEEVSRPSDDI 181
Query: 177 LDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKV-TLVDLLEEIEKIDGIERVRIGSIDP 234
L EI LA G +E+ L G + +Y G D + + +LL + IDGI+RVR + P
Sbjct: 182 LFEIAQLAAQGVREVNLLGQNVNAYRGATYDGDICSFAELLRLVAAIDGIDRVRFTTSHP 241
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
FT+D++ +L HL +QSG D L M R +TA EY+ I+ LR D+
Sbjct: 242 IEFTDDIIDVYRDTPELVSFLHLPVQSGSDRILTMMKRAHTALEYKAIIRKLRQARPDIQ 301
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
I++D IVGFPG + I ++ F YSPR GT A ++ N V +K +R
Sbjct: 302 ISSDFIVGFPGETQQDFEQTMKLVADIHFDTSYSFIYSPRPGTPAADLPNNVSEEEKKQR 361
Query: 355 SKKLIELNRINEKA--FAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVS 412
L + RI+++A + K +G V VL E G T N VN + ++
Sbjct: 362 LHILQQ--RISQQAMEISRKMVGTVQRVLVEGTSRKNVMELAGRTENNRVVNFEGSPDMI 419
Query: 413 GKILNVKI 420
GK ++V+I
Sbjct: 420 GKFVDVEI 427
>MIAB_YERPA RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 474
Score = 195 bits (495), Expect = 7e-52
Identities = 134/428 (31%), Positives = 210/428 (49%), Gaps = 13/428 (3%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVR-EGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S M + GY++ E AD+ ++NTCS+ +K ++
Sbjct: 4 KLHIKTWGCQMNEYDSSKMADLLASTHGYQLTTIPEEADLLLLNTCSIREKAQEKVFSLL 63
Query: 61 GR---ARRQNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
G+ + +NP+ II V GC A ++ VDV+ G + + +N +
Sbjct: 64 GQWKLLKEKNPQLIIGVGGCVASQEGEQLRQRAPCVDVIFGPQTLHRLPEMINHVQGTNS 123
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
V + K F+ L T AF+ I +GCN++C +C++PYTRG S+ +
Sbjct: 124 PVVDISFPEIEK-FDRLPEPRAEGPT-AFVSIMEGCNKYCTFCVVPYTRGEEVSRPSDDI 181
Query: 177 LDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKV-TLVDLLEEIEKIDGIERVRIGSIDP 234
L EI LA G +E+ L G + +Y G D + + +LL + IDGI+RVR + P
Sbjct: 182 LFEIAQLAAQGVREVNLLGQNVNAYRGATYDGDICSFAELLRLVAAIDGIDRVRFTTSHP 241
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
FT+D++ +L HL +QSG D L M R +TA EY+ I+ LR D+
Sbjct: 242 IEFTDDIIDVYRDTPELVSFLHLPVQSGSDRILTMMKRAHTALEYKAIIRKLRQARPDIQ 301
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
I++D IVGFPG + I ++ F YSPR GT A ++ N V +K +R
Sbjct: 302 ISSDFIVGFPGETQQDFEQTMKLVADIHFDTSYSFIYSPRPGTPAADLPNNVSEEEKKQR 361
Query: 355 SKKLIELNRINEKA--FAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVS 412
L + RI+++A + K +G V VL E G T N VN + ++
Sbjct: 362 LHILQQ--RISQQAMEISRKMVGTVQRVLVEGTSRKNVMELAGRTENNRVVNFEGSPDMI 419
Query: 413 GKILNVKI 420
GK ++V+I
Sbjct: 420 GKFVDVEI 427
>MIAB_RHOPT RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 463
Score = 195 bits (495), Expect = 7e-52
Identities = 131/448 (29%), Positives = 217/448 (48%), Gaps = 23/448 (5%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIG 61
K+ + GC++N Y+++ M + EG+ + AD+ ++NTC + +K +G
Sbjct: 6 KLHIKSYGCQMNVYDAQRMVDALAPEGFVETANVDDADLVILNTCHIREKASEKVYSELG 65
Query: 62 RARRQNPEAI-------IAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARD 113
R R EA IAV GC AQ E+ VDVV+G ++ + + KA
Sbjct: 66 RLRVARDEAANHGRRMQIAVAGCVAQAEGEEIIRRAPVVDVVVGPQSYHNLPQLLAKAEQ 125
Query: 114 EGKQ-------QVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRG 166
G+ + K G VL + ++ + AF+ +Q+GC++FC +C++PYTRG
Sbjct: 126 HGRALETEFPIEDKFG-VLPQPAPDAIRARGIS----AFVTVQEGCDKFCTFCVVPYTRG 180
Query: 167 SVCSKDPKKVLDEIRSLAEHGFKEIILSGIHTASYGVDLDE--KVTLVDLLEEIEKIDGI 224
+ S+ ++++++ LAE+G +E+ L G + +Y D + +L L+ + +I GI
Sbjct: 181 AEMSRPVVAIVEDVKRLAENGVREVTLIGQNVNAYHGDGPDGRAWSLGRLVRRLAEIPGI 240
Query: 225 ERVRIGSIDPTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVN 284
R+R + P +D++ L L P HL +QSG D L MNR++TA +Y +++
Sbjct: 241 VRLRYSTSHPNDVDDDLLAAHRDLPALMPFVHLPVQSGSDRILAAMNRKHTADDYRRVID 300
Query: 285 LLRDKIEDVSITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMEN 344
R E ++ ++D IVGFPG + +I + + FKYSPR GT A +M
Sbjct: 301 RFRSASETIAFSSDFIVGFPGETERDFSATLALVAQIGYAGAYSFKYSPRPGTPAADMVE 360
Query: 345 QVDGNKKDERSKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVN 404
V DER AF + IG ++VLF E G G T +
Sbjct: 361 MVPAAVMDERXXXXXXXXXXXXSAFNKAAIGRTVEVLF-ERAGRKPGQIVGRTAYLQPAH 419
Query: 405 AKADCNVSGKILNVKINSFEGEVAEGEI 432
A ++ GK+L V+++S E GE+
Sbjct: 420 VMAPDSIIGKVLPVRVDSLERYSLLGEL 447
>MIAB_PROM5 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 464
Score = 195 bits (495), Expect = 7e-52
Identities = 130/442 (29%), Positives = 212/442 (47%), Gaps = 20/442 (4%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIGRARRQ 66
T GC++N +SE M + GY AD+ + NTC++ + +K +GR ++
Sbjct: 25 TFGCQMNKADSERMAGTLEKMGYSRAIDELKADLVLYNTCTIRDSAQQKVYSFLGRQIKR 84
Query: 67 N---PEAIIAVVGCYAQI-APTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVKVG 122
P+ + V GC AQ + + + +D+++G ++ + + + D G Q V
Sbjct: 85 KHSLPKLKLVVAGCLAQQEGESLLRRVPELDLIMGPQHVNNLENLLERV-DSGNQVV--- 140
Query: 123 AVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDEIRS 182
A + + ED+ ++ ++ I GCN C+YC++P RG S+ PK + EI++
Sbjct: 141 ATEETFISEDITNARRDSTICGWVNIIYGCNERCSYCVVPSVRGKEQSRYPKAIKSEIQT 200
Query: 183 LAEHGFKEIILSGIHTASYGVDLD-------EKVTLVDLLEEIEKIDGIERVRIGSIDPT 235
LA++ +KEI L G + +YG DL ++ TL DLL I +DGI R+R + P
Sbjct: 201 LAQNNYKEITLLGQNIDAYGRDLPVTTKEGRKENTLTDLLYFIHDVDGIRRIRFSTSHPK 260
Query: 236 FFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSI 295
+F++ +++ L K+C HFH+ QSG D L+ M R YT +Y+ I+ +R + + SI
Sbjct: 261 YFSKRLIKACYELDKVCEHFHIPFQSGNDEILRLMARGYTIDKYKKIIENIRSLMPNASI 320
Query: 296 TTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERS 355
T D IV FPG + + + YSPR T A NQ+ K ER
Sbjct: 321 TADAIVAFPGETEKQYQDTLRLISDVGFDQVMTAAYSPRPNTPAAIWNNQIAEEVKKERL 380
Query: 356 KKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTR----NYIKVNAKADCNV 411
K++ EL + ++Y+ VL E S G TR ++++ + N
Sbjct: 381 KEINELVEATSRKRNQRYLNNTESVLIEGLNPKNSMQMMGRTRTNRLTFVEIPENIEFNY 440
Query: 412 S-GKILNVKINSFEGEVAEGEI 432
S G LNVKI G+I
Sbjct: 441 SFGDELNVKITEARSFSLSGQI 462
>MIAB_BIFAA RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 480
Score = 195 bits (495), Expect = 7e-52
Identities = 123/370 (33%), Positives = 188/370 (50%), Gaps = 12/370 (3%)
Query: 7 TLGCRVNTYESEAMTEKFVREGY----EVVDFNEMADVYVVNTCSVTNMGDKKSRQIIG- 61
TLGC++N ++SE + +GY E N D+ V+NTC+V ++ IG
Sbjct: 31 TLGCQMNVHDSERIAGVLEADGYVPATEEQYLNHDIDLIVMNTCAVRENAAERMYGTIGL 90
Query: 62 --RARRQNPEAIIAVVGCYAQIAPTEVSG-IEGVDVVLGSRNKGEIVYYVNKARDEGKQQ 118
+R+ P IAV GC AQ+ +++ VD V G++N G + +++AR EG Q
Sbjct: 91 WAELKRERPNLQIAVGGCMAQLDREKIAKKAPWVDAVFGTKNIGSLPQLLDQARIEGHAQ 150
Query: 119 VKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLD 178
VKV L F + +K +++ I GCN C +C++P TRG + P +L
Sbjct: 151 VKVQEELN--YFPSQLPTDRASKVSSWVAISVGCNNTCTFCIVPTTRGKEHDRRPGDILA 208
Query: 179 EIRSLAEHGFKEIILSGIHTASYGVDLDEKVTLVDLLEEIEKIDGIERVRIGSIDPTFFT 238
EIR + G KE+ L G + S+G + ++ LL +IDG+ERVR S P FT
Sbjct: 209 EIRQCVDEGAKEVTLLGQNVNSFGYGIGDRFAFSKLLRACGEIDGLERVRFTSPHPAAFT 268
Query: 239 EDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITTD 298
+DV+ + + H LQSG D L+ M R Y + ++ DI+ +R+ + D I+TD
Sbjct: 269 DDVIAAMAETPNVMHQLHFPLQSGSDRILRAMRRSYRSAKFLDILRKIREAMPDAQISTD 328
Query: 299 VIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSKKL 358
+IVGFPG ++ + F YSPR GT A ME QV + +R ++L
Sbjct: 329 IIVGFPGETEEDFQETLRVVEEARFASAFTFIYSPRPGTPAAEME-QVPHDVVQDRFERL 387
Query: 359 IEL-NRINEK 367
+ L RI E+
Sbjct: 388 VALQERITEE 397
>MIAB_TROWT RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 470
Score = 194 bits (494), Expect = 9e-52
Identities = 121/385 (31%), Positives = 200/385 (51%), Gaps = 7/385 (1%)
Query: 1 MKVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
M T GC++N ++SE + EGY V ++ ADV V+NTC+V D + +
Sbjct: 1 MTYTVRTYGCQMNVHDSERIAGVLEDEGY-VKSGSDDADVVVLNTCAVRENADNRFYGNL 59
Query: 61 GRA-RRQNPEAI--IAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
G+ +++N I IAV GC AQ ++ VDVV G+ N G + + ++R
Sbjct: 60 GQLLQKKNNGRIRQIAVGGCLAQKDRHKIFEKAPWVDVVFGTHNLGSLPALLRRSRHNKT 119
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
++++ L+ L + +N + A++ I GCN C +C++P RG + P +
Sbjct: 120 AEIEIKDFLETFP-SSLPVRRESNYS-AWVSISVGCNNTCTFCIVPSLRGKERDRRPGDI 177
Query: 177 LDEIRSLAEHGFKEIILSGIHTASYGVDLDEKVTLVDLLEEIEKIDGIERVRIGSIDPTF 236
L EI +L G E+ L G + +YGV+ ++ LL + I+G+ER++ S P
Sbjct: 178 LAEISALVSEGVLEVTLLGQNVNTYGVEFGDRSAFASLLRKAGAIEGLERLKFTSPHPAA 237
Query: 237 FTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSIT 296
FT DV+ + + + P H+ LQSG D L+ M R Y A ++ I++ R+KI +++IT
Sbjct: 238 FTSDVIDAMHDTQAVLPQLHMPLQSGSDRILRAMRRSYRAGKFLKIISEARNKIPNIAIT 297
Query: 297 TDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSK 356
TD+IVGFPG + + + F+YSPR GT A +M NQ+ G+ ER +
Sbjct: 298 TDIIVGFPGETEEDFQDTLNLVAEVRFASAFTFQYSPRPGTPAASMPNQIPGDIVQERYE 357
Query: 357 KLIELNRINEKAFAEKYIGEVIDVL 381
+L++L K IG+ +++L
Sbjct: 358 RLLDLQNRIALEENRKLIGKEVELL 382
>MIAB_THIDA RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 446
Score = 194 bits (494), Expect = 9e-52
Identities = 137/439 (31%), Positives = 204/439 (46%), Gaps = 9/439 (2%)
Query: 2 KVAFATLGCRVNTYESEAMTEKF-VREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S+ M + EG E ADV + NTCSV +K +
Sbjct: 3 KIYIKTFGCQMNEYDSDKMADVLNAAEGLTPTSTPEDADVILFNTCSVREKAQEKVFHDL 62
Query: 61 GRAR---RQNPEAIIAVVGCYA-QIAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR R + NP II V GC A Q V+ VDVV G + + + + R+ G+
Sbjct: 63 GRVRHLKQANPNLIIGVGGCVASQEGAAIVARAPFVDVVFGPQTLHRLPELIAQRRETGR 122
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
QV + K F+ L AF+ I +GC+++C +C++PYTRG S+ V
Sbjct: 123 AQVDISFPEIEK-FDHLPPARVEGGA-AFVSIMEGCSKYCTFCVVPYTRGEEVSRPFDDV 180
Query: 177 LDEIRSLAEHGFKEIILSGIHTASYGVDLDEKVT--LVDLLEEIEKIDGIERVRIGSIDP 234
+ E+ +LA G KEI L G + +Y LDE T LLE + +I G+ER+R + P
Sbjct: 181 IAEVANLAARGVKEITLLGQNVNAYQGALDEGGTADFAFLLEMVHEIPGVERIRYTTSHP 240
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
T+ ++ L KL H HL +QSG D L M R +T E++ IV LR++ D+
Sbjct: 241 REMTQRLIECYGKLPKLVSHLHLPVQSGSDRILAAMKRGHTVLEFKSIVRKLREQRSDLC 300
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
+++D IVGFPG ++ + + F YS R GT A + + V K +R
Sbjct: 301 LSSDFIVGFPGETGADFEATMKLVEELNFDASFSFIYSKRPGTPAADYADDVSAELKTQR 360
Query: 355 SKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSGK 414
+L + +G V VL E + + G T N VN + G+
Sbjct: 361 LMRLQARIEAQAQGVNRSMVGSVQRVLVEGQARKNANELAGRTGNNRIVNFAGPSRLLGQ 420
Query: 415 ILNVKINSFEGEVAEGEIV 433
++V I GE V
Sbjct: 421 FVDVTITQALPHSLRGEAV 439
>MIAB_PASMU RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 474
Score = 194 bits (493), Expect = 1e-51
Identities = 140/444 (31%), Positives = 217/444 (48%), Gaps = 18/444 (4%)
Query: 2 KVAFATLGCRVNTYESEAMTEKF-VREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S M + G E+ + E ADV ++NTCS+ +K +
Sbjct: 4 KLHIKTWGCQMNEYDSSKMADLLNSTHGLELTEIPEEADVLLLNTCSIREKAQEKVFHQL 63
Query: 61 GR---ARRQNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR ++ P +I V GC A + + VD++ G + + +N+ R GK
Sbjct: 64 GRWKELKKHKPGLVIGVGGCVASQEGEHIRTRAPYVDIIFGPQTLHRLPEMINQIRG-GK 122
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
V + + + F+ L T AF+ I +GCN++C++C++PYTRG S+ V
Sbjct: 123 SSVVDVSFPEIEKFDRLPEPRAEGPT-AFVSIMEGCNKYCSFCVVPYTRGEEVSRPVDDV 181
Query: 177 LDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKV-TLVDLLEEIEKIDGIERVRIGSIDP 234
L EI LAE G +E+ L G + +Y G D+ + T +LL + IDGI+R+R + P
Sbjct: 182 LFEIAQLAEQGVREVNLLGQNVNAYRGATHDDGICTFAELLRLVAAIDGIDRLRFTTSHP 241
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
FT+D++ +L HL +QSG D L M R +TA EY+ I+ LR ++
Sbjct: 242 IEFTDDIIDVYRDTPELVSFLHLPVQSGSDRVLSMMKRNHTALEYKSIIRKLRAVRPEIQ 301
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
I++D IVGFPG + ++ + F YS R GT A +M + V +K +R
Sbjct: 302 ISSDFIVGFPGETAEDFEQTMNLIAQVNFDMSFSFIYSARPGTPAADMPDDVTEEEKKQR 361
Query: 355 SKKLIELNRINEKA--FAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVS 412
L + RIN +A F+ +G VL E + TG T VN ++
Sbjct: 362 LYVLQQ--RINNQAAQFSRAMLGTEQRVLVEGPSKKDLMELTGRTETNRIVNFVGTPDMI 419
Query: 413 GKILNVKI-----NSFEGEVAEGE 431
GK +++KI NS GEV E
Sbjct: 420 GKFVDIKITDVFTNSLRGEVVRTE 443
>MIAB_ORITI RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 449
Score = 194 bits (493), Expect = 1e-51
Identities = 134/444 (30%), Positives = 226/444 (50%), Gaps = 25/444 (5%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIGRARRQ 66
T GC++N Y+S M + +GY +V A V ++NTC + +K +GR ++
Sbjct: 9 TYGCQMNVYDSNRMVDLLETQGYNIVANMADASVIILNTCHIREKASEKMYSELGRIKKL 68
Query: 67 NP--------EAIIAVVGCYAQIAPTEVSGIE-GVDVVLGSRNKGEIVYYVNKARDEGKQ 117
+A I V GC Q E+ E V++++G ++ + + K D G +
Sbjct: 69 QQSRLKAGKSKAKIIVAGCVGQAEGEEIFIREPAVNIIVGPQSYYNLPTMLEKL-DSGTE 127
Query: 118 QVKVG-----AVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKD 172
+ A NK+ E LK + + +Q+GC++FC +C++PYTRG+ S+
Sbjct: 128 NHLIDLDFVEAAKFNKLPEVLK----SPTVSGLVSVQEGCDKFCTFCVVPYTRGAEFSRP 183
Query: 173 PKKVLDEIRSLAEHGFKEIILSGIHTASY-GVDLDEK-VTLVDLLEEIEKIDGIERVRIG 230
++V E+ ++A+ G KE++L G + ++Y G D + K +L DL++ + KID I+R+R
Sbjct: 184 LEQVYREVLNIAQQGAKEVVLVGQNVSAYHGKDENGKECSLADLIKYVAKIDKIKRIRYI 243
Query: 231 SIDPTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKI 290
+ P T+ ++ +KL P HL +QSG + LK MNRR++ + Y +I+ LR+
Sbjct: 244 TSHPNDMTDQLLSLHATEEKLMPFLHLPVQSGSNKILKLMNRRHSRERYLEIIQQLRELR 303
Query: 291 EDVSITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNK 350
D+ I++D+IVGFPG K + + FKYS R GT A ++ Q+
Sbjct: 304 PDIVISSDIIVGFPGEDDEDFEATLSLAKEARFGQCYSFKYSQRPGTPAA-VKQQISEEV 362
Query: 351 KDERSKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVY--TGYTRNYIKVNAKAD 408
K R L + +K IG+V+ VLF+ + + + T Y ++ +N K D
Sbjct: 363 KQHRLSILQAQLMQQQLECNQKLIGKVVPVLFDRDGKYDGQIIGKTPYMQSVCIMNEK-D 421
Query: 409 CNVSGKILNVKINSFEGEVAEGEI 432
N+ GKI+NVKI + GE+
Sbjct: 422 NNLYGKIVNVKILTASASSLFGEV 445
>MIAB_NOCSJ RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 496
Score = 194 bits (493), Expect = 1e-51
Identities = 121/384 (31%), Positives = 191/384 (49%), Gaps = 10/384 (2%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIGR---A 63
T GC++N ++SE +T GY + ADV V NTC+V D K +G
Sbjct: 16 TYGCQMNVHDSERLTGLLEDAGYVAAPDGQQADVVVFNTCAVRENADNKLYGNLGHLAPV 75
Query: 64 RRQNPEAIIAVVGCYAQI-APTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVKVG 122
+ P IAV GC AQ T + VDVV G+ N G + + +AR + + QV++
Sbjct: 76 KAATPGMQIAVGGCLAQKDRDTITTRAPWVDVVFGTHNIGSLPALLERARVQDEAQVEIL 135
Query: 123 AVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDEIRS 182
L+ VF + A++ + GCN C +C++P RG + P ++L E+ +
Sbjct: 136 ESLE--VFPSTLPTRRESAYAAWVSVSVGCNNTCTFCIVPSLRGKEKDRRPGEILAEVEA 193
Query: 183 LAEHGFKEIILSGIHTASYGVDLDEKVTLVDLLEEIEKIDGIERVRIGSIDPTFFTEDVV 242
L G EI L G + +YGV+ ++ LL ++DG+ERVR S P FT+DV+
Sbjct: 194 LVADGVSEITLLGQNVNAYGVEFGDRQAFSKLLRACGEVDGLERVRFTSPHPAEFTDDVI 253
Query: 243 RRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITTDVIVG 302
+ + P H+ LQSG D L+ M R Y +++ I+ +R + D +ITTD+IVG
Sbjct: 254 AAMAETPNVMPQLHMPLQSGSDKVLRDMRRSYRQRKFLGILERVRAAMPDAAITTDIIVG 313
Query: 303 FPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSKKLIELN 362
FPG ++ S F+YS R GT A + +QV +R ++L+ L
Sbjct: 314 FPGETEEDFLQTMHVVRAARFSGAFTFQYSKRPGTPAATLPDQVPPEVVKDRYERLVAL- 372
Query: 363 RINEKAFAE--KYIGEVIDVLFEE 384
+NE A+ E + +G ++++ E
Sbjct: 373 -VNEIAWEENKRLVGRRVELMVAE 395
>MIAB_ENTS8 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 474
Score = 194 bits (493), Expect = 1e-51
Identities = 136/444 (30%), Positives = 214/444 (48%), Gaps = 18/444 (4%)
Query: 2 KVAFATLGCRVNTYESEAMTEKF-VREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S M + G+ + D E AD+ ++NTCS+ +K +
Sbjct: 4 KLHIKTWGCQMNEYDSSKMADLLETTHGFTLTDVAEEADILLLNTCSIREKAQEKVFHQL 63
Query: 61 GR---ARRQNPEAIIAVVGCYAQIAPTEVSG-IEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR + +NP+ II V GC A + VD++ G + + +N+ +
Sbjct: 64 GRWKTLKEKNPDVIIGVGGCVASQEGDHIRDRARYVDIIFGPQTLHRLPEMINQVKGTRS 123
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
V + K F+ L T AF+ I +GCN++C YC++PYTRG S+ +
Sbjct: 124 PVVDISFPEIEK-FDRLPEPRAEGPT-AFVSIMEGCNKYCTYCVVPYTRGEEVSRPSDDI 181
Query: 177 LDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKV-TLVDLLEEIEKIDGIERVRIGSIDP 234
+ EI LA G +E+ L G + ++ G + D T DLL + IDGI+R+R + P
Sbjct: 182 VLEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGTFADLLRLVAAIDGIDRIRFTTSHP 241
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
FT+D++ +L HL +QSG D L M R +TA EY+ I+ LR D+
Sbjct: 242 IEFTDDIIEVYRDTPELVSFLHLPVQSGSDRVLNMMGRTHTALEYKAIIRKLRAARPDIQ 301
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
I++D IVGFPG + + ++ F +S R GT A +M + V +K +R
Sbjct: 302 ISSDFIVGFPGETQQDFEQTMKLIAEVNFDMSYSFIFSARPGTPAADMVDDVPEEEKKQR 361
Query: 355 SKKLIELNRINEKAFA--EKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVS 412
L E RIN++A A + +G +L E +G T N VN + ++
Sbjct: 362 LYILQE--RINQQAMAWSRRMLGTTQRILVEGTSRKSIMELSGRTENNRVVNFEGSPDMV 419
Query: 413 GKILNVKI-----NSFEGEVAEGE 431
GK ++V+I NS G+V E
Sbjct: 420 GKFVDVEITEVFPNSLRGKVIRTE 443
>MIAB_YERPY RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 474
Score = 194 bits (492), Expect = 2e-51
Identities = 132/428 (30%), Positives = 211/428 (49%), Gaps = 13/428 (3%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVR-EGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S M + GY++ E AD+ ++NTCS+ +K ++
Sbjct: 4 KLHIKTWGCQMNEYDSSKMADLLASTHGYQLTTIPEEADLLLLNTCSIREKAQEKVFSLL 63
Query: 61 GR---ARRQNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
G+ + +NP+ II V GC A ++ VDV+ G + + +N ++
Sbjct: 64 GQWKLLKEKNPQLIIGVGGCVASQEGEQLRQRAPCVDVIFGPQTLHRLPEMINHVQETNS 123
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
V + K F+ L T AF+ I +GCN++C +C++PYTRG S+ +
Sbjct: 124 PVVDISFPEIEK-FDRLPEPRAEGPT-AFVSIMEGCNKYCTFCVVPYTRGEEVSRPSDDI 181
Query: 177 LDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKV-TLVDLLEEIEKIDGIERVRIGSIDP 234
L EI LA G +E+ L G + +Y G D + + +LL + IDGI+R+R + P
Sbjct: 182 LFEIAQLAAQGVREVNLLGQNVNAYRGATYDGDICSFAELLRLVAAIDGIDRIRFTTSHP 241
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
FT+D++ +L HL +QSG D L M R +TA EY+ I+ LR D+
Sbjct: 242 IEFTDDIIDVYRDTPELVSFLHLPVQSGSDRILTMMKRAHTALEYKAIIRKLRQARPDIQ 301
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
I++D IVGFPG + I ++ F YSPR GT A ++ + V +K +R
Sbjct: 302 ISSDFIVGFPGETQQDFEQTMKLVADIHFDTSYSFIYSPRPGTPAADLPDNVSEEEKKQR 361
Query: 355 SKKLIELNRINEKA--FAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVS 412
L + RI+++A + K +G V VL E G T N VN + ++
Sbjct: 362 LHILQQ--RISQQAMEISRKMVGTVQRVLVEGTSRKNVMELAGRTENNRVVNFEGSPDMI 419
Query: 413 GKILNVKI 420
GK ++V+I
Sbjct: 420 GKFVDVEI 427
>MIAB_TROW8 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 470
Score = 194 bits (492), Expect = 2e-51
Identities = 121/385 (31%), Positives = 199/385 (51%), Gaps = 7/385 (1%)
Query: 1 MKVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
M T GC++N ++SE + EGY V ++ ADV V+NTC+V D + +
Sbjct: 1 MTYTVRTYGCQMNVHDSERIAGVLEDEGY-VKSGSDDADVVVLNTCAVRENADNRFYGNL 59
Query: 61 GRA-RRQNPEAI--IAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
G+ +++N I IAV GC AQ ++ VDVV G+ N G + + ++R
Sbjct: 60 GQLLQKKNNGRIRQIAVGGCLAQKDRHKIFEKAPWVDVVFGTHNLGSLPALLRRSRHNKT 119
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
++++ L+ L + +N + A++ I GCN C +C++P RG + P +
Sbjct: 120 AEIEIKDFLETFP-SSLPVRRESNYS-AWVSISVGCNNTCTFCIVPSLRGKERDRRPGDI 177
Query: 177 LDEIRSLAEHGFKEIILSGIHTASYGVDLDEKVTLVDLLEEIEKIDGIERVRIGSIDPTF 236
L EI +L G E+ L G + +YGV+ ++ LL + I+G+ER++ S P
Sbjct: 178 LAEISALVSEGVLEVTLLGQNVNTYGVEFGDRSAFASLLRKAGAIEGLERLKFTSPHPAA 237
Query: 237 FTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSIT 296
FT DV+ + + + P H+ LQSG D L+ M R Y A ++ I++ R+KI +++IT
Sbjct: 238 FTSDVIDAMHDTQAVLPQLHMPLQSGSDRILRAMRRSYRAGKFLKIISEARNKIPNIAIT 297
Query: 297 TDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSK 356
TD+IVGFPG + + + F+YSPR GT A +M NQ+ G+ ER
Sbjct: 298 TDIIVGFPGETEEDFQDTLNLVAEVRFASAFTFQYSPRPGTPAASMPNQIPGDIVQERYD 357
Query: 357 KLIELNRINEKAFAEKYIGEVIDVL 381
+L++L K IG+ +++L
Sbjct: 358 RLLDLQNRIALEENRKLIGKEVELL 382
>MIAB_DESVV RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 449
Score = 194 bits (492), Expect = 2e-51
Identities = 123/383 (32%), Positives = 198/383 (51%), Gaps = 8/383 (2%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIGRARR- 65
T GC++N +S+ + + G+ F E A + +VNTCSV + ++K ++GR R+
Sbjct: 10 TFGCQMNVNDSDWLARALMERGFSPAPFGE-ARLTIVNTCSVRDKPEQKVYSLLGRIRQA 68
Query: 66 --QNPEAIIAVVGCYAQ-IAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVKVG 122
+ P+A +AV GC AQ I S V +V G+ +++ +E ++ +
Sbjct: 69 TGKKPDAFVAVGGCVAQQIGSGFFSRFPQVRLVFGTDGLAMAPQALDRLVEEPDLKLSLL 128
Query: 123 AVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDEIRS 182
++ D + + F+ I GC+ FCAYC++PYTRG S+ +LDE R+
Sbjct: 129 DFSEDYPERDAVLGQGAVPASVFVNIMQGCDNFCAYCIVPYTRGRQKSRATGTILDECRA 188
Query: 183 LAEHGFKEIILSGIHTASYGVDLD-EKVTLVDLLEEIEKIDGIERVRIGSIDPTFFTEDV 241
L + G +EI L G + S+G D + T LL ++ + G+ER+R + P +V
Sbjct: 189 LLDRGAREITLLGQNVNSFGQDSHGDGTTFAQLLHKVAALPGLERLRFVTPHPKDIAPEV 248
Query: 242 VRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITTDVIV 301
V L LCP HL LQ+G D LK M RRY Y IV+ LR D+ +++D+IV
Sbjct: 249 VEAFGTLPNLCPRLHLPLQAGSDRILKLMGRRYDMARYLRIVDDLRAARPDIVLSSDIIV 308
Query: 302 GFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSKKLIEL 361
GFPG L+ + + ++ F YS R GTRAE + +++ K ER ++L L
Sbjct: 309 GFPGETEEDFMETMGALETVGYAASYSFCYSDRPGTRAEMLPDKLSREVKLERLERLQTL 368
Query: 362 -NRINEKAFAEKYIGEVIDVLFE 383
NR+ E+ + +G+ ++VL E
Sbjct: 369 QNRLTERCL-QDMVGKKVEVLLE 390
>MIAB_MYCGI RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 512
Score = 193 bits (491), Expect = 2e-51
Identities = 132/438 (30%), Positives = 203/438 (46%), Gaps = 22/438 (5%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKK---SRQIIGRA 63
T GC++N ++SE ++ + GY+ AD+ V NTC+V D K + +
Sbjct: 27 TYGCQMNVHDSERLSGLLEQAGYQRAGAGVDADIVVFNTCAVRENADNKLYGNLSHLAPR 86
Query: 64 RRQNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVKVG 122
++ +P+ IAV GC AQ V VDVV G+ N G + +++AR QV++
Sbjct: 87 KQADPQMQIAVGGCLAQKDRDAVLRKAPWVDVVFGTHNIGSLPTLLDRARHNRVAQVEIA 146
Query: 123 AVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDEIRS 182
L+ F + A++ I GCN C +C++P RG + P VL E+++
Sbjct: 147 ESLRE--FPSALPASRESAYAAWVSISVGCNNTCTFCIVPSLRGKEVDRRPGDVLAEVQA 204
Query: 183 LAEHGFKEIILSGIHTASYGVDLDEKVTLVD------LLEEIEKIDGIERVRIGSIDPTF 236
L + G EI L G + +YGV + D LL +IDG+ERVR S P
Sbjct: 205 LVDQGVLEITLLGQNVNAYGVSFADPTEARDRGAFAKLLRACGRIDGLERVRFTSPHPAE 264
Query: 237 FTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSIT 296
FT+DV+ + +CP H+ LQSG D L+ M R Y A Y I++ +R I +IT
Sbjct: 265 FTDDVIEAMAQTSNVCPTLHMPLQSGSDRILRAMRRSYRADRYLGIIDRVRTAIPHAAIT 324
Query: 297 TDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSK 356
TD+IVGFPG ++ S F+YS R GT A +E Q+ ER +
Sbjct: 325 TDLIVGFPGETEEDFQATLDVVEAARFSSAFTFQYSKRPGTPAAELEGQIPKAVVSERYQ 384
Query: 357 KLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSGKIL 416
+LIEL IG +++L V TG R + G+++
Sbjct: 385 RLIELQERISWEENRAQIGREVELL----------VATGEGRKDAATARMSGRARDGRLV 434
Query: 417 NVKINSFEGEVAEGEIVL 434
+ + ++ G+IV+
Sbjct: 435 HFAPGALGADIRPGDIVV 452
>MIAB_DESVH RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 449
Score = 193 bits (491), Expect = 2e-51
Identities = 123/383 (32%), Positives = 197/383 (51%), Gaps = 8/383 (2%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIGRARR- 65
T GC++N +S+ + + G+ F E A + +VNTCSV + ++K ++GR R+
Sbjct: 10 TFGCQMNVNDSDWLARALMERGFSPAPFGE-ARLTIVNTCSVRDKPEQKVYSLLGRIRQA 68
Query: 66 --QNPEAIIAVVGCYAQ-IAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVKVG 122
+ P+A +AV GC AQ I S V +V G+ +++ +E ++ +
Sbjct: 69 TSKKPDAFVAVGGCVAQQIGSGFFSRFPQVRLVFGTDGLAMAPQALDRLVEEPDLKLSLL 128
Query: 123 AVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDEIRS 182
++ D + + F+ I GC+ FCAYC++PYTRG S+ +LDE R+
Sbjct: 129 DFSEDYPERDAVLGQGAVPASVFVNIMQGCDNFCAYCIVPYTRGRQKSRATGTILDECRA 188
Query: 183 LAEHGFKEIILSGIHTASYGVDLD-EKVTLVDLLEEIEKIDGIERVRIGSIDPTFFTEDV 241
L + G +EI L G + S+G D + T LL ++ + G+ER+R + P +V
Sbjct: 189 LLDRGAREITLLGQNVNSFGQDSHGDGTTFAQLLHKVAALPGLERLRFVTPHPKDIAPEV 248
Query: 242 VRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITTDVIV 301
V L LCP HL LQ+G D LK M RRY Y IV+ LR D+ +++D+IV
Sbjct: 249 VEAFGTLPNLCPRLHLPLQAGSDRILKLMGRRYDMARYLRIVDDLRAARPDIVLSSDIIV 308
Query: 302 GFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSKKLIEL 361
GFPG L+ + + ++ F YS R GTRAE + +++ K ER ++L L
Sbjct: 309 GFPGETEEDFMETMDALETVGYAASYSFCYSDRPGTRAEMLPDKLSREVKLERLERLQTL 368
Query: 362 -NRINEKAFAEKYIGEVIDVLFE 383
NR+ E+ + +G ++VL E
Sbjct: 369 QNRLTERCL-QDMVGRKVEVLLE 390
>MIAB_YERPP RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 474
Score = 193 bits (490), Expect = 3e-51
Identities = 133/428 (31%), Positives = 210/428 (49%), Gaps = 13/428 (3%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVR-EGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S M + GY++ E AD+ ++NTCS+ +K ++
Sbjct: 4 KLHIKTWGCQMNEYDSSKMADLLASTHGYQLTTIPEEADLLLLNTCSIREKAQEKVFSLL 63
Query: 61 GR---ARRQNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
G+ + +NP+ II V GC A ++ VDV+ G + + +N +
Sbjct: 64 GQWKLLKEKNPQLIIGVGGCVASQEGEQLRQRAPCVDVIFGPQTLHRLPEMINHVQGTNS 123
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
V + K F+ L T AF+ I +GCN++C +C++PYTRG S+ +
Sbjct: 124 PVVDISFPEIEK-FDRLPEPRAEGPT-AFVSIMEGCNKYCTFCVVPYTRGEEVSRPSDDI 181
Query: 177 LDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKV-TLVDLLEEIEKIDGIERVRIGSIDP 234
L EI LA G +E+ L G + +Y G D + + +LL + IDGI+RVR + P
Sbjct: 182 LFEIAQLAAQGVREVNLLGQNVNAYRGATYDGDICSFAELLRLVAAIDGIDRVRFTTSHP 241
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
FT+D++ +L HL +QSG D L M R +TA EY+ I+ LR D+
Sbjct: 242 IEFTDDIIDVYRDTPELVSFLHLPVQSGSDRILTMMKRAHTALEYKAIIRKLRQARPDIQ 301
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
I++D IVGFPG + I ++ F YSPR GT A ++ + V +K +R
Sbjct: 302 ISSDFIVGFPGETQQDFEQTMKLVADIHFDTSYSFIYSPRPGTPAADLPDNVSEEEKKQR 361
Query: 355 SKKLIELNRINEKA--FAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVS 412
L + RI+++A + K +G V VL E G T N VN + ++
Sbjct: 362 LHILQQ--RISQQAMEISRKMVGTVQRVLVEGTSRKNVMELAGRTENNRVVNFEGSPDMI 419
Query: 413 GKILNVKI 420
GK ++V+I
Sbjct: 420 GKFVDVEI 427
>MIAB_YERPG RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 474
Score = 193 bits (490), Expect = 3e-51
Identities = 133/428 (31%), Positives = 210/428 (49%), Gaps = 13/428 (3%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVR-EGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S M + GY++ E AD+ ++NTCS+ +K ++
Sbjct: 4 KLHIKTWGCQMNEYDSSKMADLLASTHGYQLTTIPEEADLLLLNTCSIREKAQEKVFSLL 63
Query: 61 GR---ARRQNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
G+ + +NP+ II V GC A ++ VDV+ G + + +N +
Sbjct: 64 GQWKLLKEKNPQLIIGVGGCVASQEGEQLRQRAPCVDVIFGPQTLHRLPEMINHVQGTNS 123
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
V + K F+ L T AF+ I +GCN++C +C++PYTRG S+ +
Sbjct: 124 PVVDISFPEIEK-FDRLPEPRAEGPT-AFVSIMEGCNKYCTFCVVPYTRGEEVSRPSDDI 181
Query: 177 LDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKV-TLVDLLEEIEKIDGIERVRIGSIDP 234
L EI LA G +E+ L G + +Y G D + + +LL + IDGI+RVR + P
Sbjct: 182 LFEIAQLAAQGVREVNLLGQNVNAYRGATYDGDICSFAELLRLVAAIDGIDRVRFTTSHP 241
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
FT+D++ +L HL +QSG D L M R +TA EY+ I+ LR D+
Sbjct: 242 IEFTDDIIDVYRDTPELVSFLHLPVQSGSDRILTMMKRAHTALEYKAIIRKLRQARPDIQ 301
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
I++D IVGFPG + I ++ F YSPR GT A ++ + V +K +R
Sbjct: 302 ISSDFIVGFPGETQQDFEQTMKLVADIHFDTSYSFIYSPRPGTPAADLPDNVSEEEKKQR 361
Query: 355 SKKLIELNRINEKA--FAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVS 412
L + RI+++A + K +G V VL E G T N VN + ++
Sbjct: 362 LHILQQ--RISQQAMEISRKMVGTVQRVLVEGTSRKNVMELAGRTENNRVVNFEGSPDMI 419
Query: 413 GKILNVKI 420
GK ++V+I
Sbjct: 420 GKFVDVEI 427
>MIAB_WOLPP RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 440
Score = 193 bits (490), Expect = 3e-51
Identities = 127/385 (32%), Positives = 199/385 (51%), Gaps = 7/385 (1%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIGR--AR 64
T GC++N Y+S M G+ VV E AD+ ++NTC + +K +GR +
Sbjct: 8 TYGCQMNVYDSILMENIVKPLGFNVVSDAEQADLVILNTCHIREKAAEKLYSELGRIHSS 67
Query: 65 RQNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVKVGA 123
++N E I V GC AQ EV VD+V+G ++ + + KA K V
Sbjct: 68 QKNKEMTIVVAGCVAQAEGEEVFRRAPFVDIVVGPQSIPALPELIVKA-SRSKGHVINTD 126
Query: 124 VLKNKVFEDLKIEEYNNK--TRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDEIR 181
+ F+ L E Y N + AFL IQ+GC++FC +C++PYTRG+ S+ ++ E
Sbjct: 127 FPEVAKFDKLPDECYGNSQGSSAFLAIQEGCDKFCTFCVVPYTRGAEYSRPVNEIFREAL 186
Query: 182 SLAEHGFKEIILSGIHTASYGVDLDEKV-TLVDLLEEIEKIDGIERVRIGSIDPTFFTED 240
+L ++G KEI L G + +Y + + +V L L+ I KI+ +ER+R + P E
Sbjct: 187 NLVKNGAKEITLLGQNVNAYHGECEGEVWDLGKLISHIAKIEKLERIRYTTSHPRDMHES 246
Query: 241 VVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITTDVI 300
+ KL P HL +QSG + L MNR++TA+EY I++ LR ++ ++D I
Sbjct: 247 LYLVHAEESKLMPFVHLPVQSGSNKILHAMNRKHTAEEYLGIIDRLRKLKPEIEFSSDFI 306
Query: 301 VGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSKKLIE 360
VGFPG ++++ ++ + FKYSPR GT ++QV K ER +L E
Sbjct: 307 VGFPGETEKDFEETMKLVEKVKYAQAYSFKYSPRPGTPGAERKDQVPEEVKTERLLRLQE 366
Query: 361 LNRINEKAFAEKYIGEVIDVLFEEE 385
L + F + IG+ I VLF ++
Sbjct: 367 LISKQQLEFNQSMIGKTIPVLFSDK 391
>MIAB1_MYCMM RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB 1;
EC=2.-.-.-;AltName: Full=tRNA-i(6)A37
methylthiotransferase 1;
Length = 529
Score = 193 bits (490), Expect = 3e-51
Identities = 133/444 (29%), Positives = 207/444 (46%), Gaps = 29/444 (6%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKK---SRQIIGRA 63
TLGC +N ++SE M GY D AD+ V NTC+V D K S +
Sbjct: 31 TLGCLMNAHDSERMAGLLEDAGYIKADPGAPADLVVFNTCAVRENADNKLYGSLAHLAPI 90
Query: 64 RRQNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVKVG 122
+ P IAV GC AQ V VDVV G+ N G + + ++R QV++
Sbjct: 91 KASRPGMQIAVGGCLAQKDRHIVLDRAPWVDVVFGTHNIGSLPVLLERSRHNQDAQVEIL 150
Query: 123 AVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDEIRS 182
L+ F ++ A++ I GCN C +C++P RG + P +L E+++
Sbjct: 151 ESLRT--FPSALPAARDSAYAAWVSISVGCNNSCTFCIVPSLRGKEADRRPGDILAEVQA 208
Query: 183 LAEHGFKEIILSGIHTASYGVDLDE-------------KVTLVDLLEEIEKIDGIERVRI 229
L E G E+ L G + SYGV+ + + LL +I+G+ER+R
Sbjct: 209 LVEQGVLEVTLLGQNVNSYGVNFADPDPSTGEEPLPRDRGAFAQLLRACGRIEGLERIRF 268
Query: 230 GSIDPTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDK 289
S P FT+DV+ + +CPH H+ LQSG D LK M R Y Y I++ +R
Sbjct: 269 TSPHPAEFTDDVILAMAETPAVCPHLHMPLQSGSDQILKAMRRSYRRDRYLGIIDKVRAA 328
Query: 290 IEDVSITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGN 349
I +ITTD+IVGFPG +++ + F+YSPR GT A +M +Q+ N
Sbjct: 329 IPHAAITTDIIVGFPGETEHDFEQTLDVVQKARFTSAFTFQYSPRPGTPAADMPDQIPKN 388
Query: 350 KKDERSKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADC 409
+R ++L+ L +G ++L V G G T +++ +A
Sbjct: 389 VVQQRFERLVALQERISLESNRSLVGTRQELL----VVAGEGRKNATT---ARISGRAR- 440
Query: 410 NVSGKILNVKINSFEGEVAEGEIV 433
G++++ + +S +G V G++V
Sbjct: 441 --DGRLVHFRPDSADGSVRPGDLV 462
>Y416_RICPR RecName: Full=Putative methylthiotransferase RP416;
EC=2.-.-.-;
Length = 421
Score = 192 bits (489), Expect = 4e-51
Identities = 121/406 (29%), Positives = 198/406 (48%), Gaps = 26/406 (6%)
Query: 1 MKVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
++ T GCR+N YESE + + G + +V + NTC+VT
Sbjct: 10 LRQEIVTFGCRLNIYESEIIRKNLELSGLD--------NVAIFNTCAVTKSXXXXXXXXX 61
Query: 61 GRARRQNPEAIIAVVGCYAQIAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVK 120
NP+ I V GC AQ P + VD V+G+ K YY Q++
Sbjct: 62 XXXXXNNPDLKIIVTGCSAQANPKMYGNMSEVDKVIGNEEKLLSHYY-----QITDQKIS 116
Query: 121 VGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDEI 180
V ++ K + ++ K+RAF+++Q+GC+ C +C+IPY RG S ++ ++
Sbjct: 117 VNDIMSVKETACHLVSSFDGKSRAFIQVQNGCDHNCTFCIIPYVRGKSRSIPIGTIVAQV 176
Query: 181 RSLAEHGFKEIILSGIHTASYGVDLDEKVTLVDLLEEIEK-IDGIERVRIGSIDPTFFTE 239
+ L GFKE++++G+ +YG DL T +++ + K + ++R+R+ SID +
Sbjct: 177 KHLVLKGFKEVVITGVDVTAYGSDLPGSPTFAQMIKRVLKLVPELKRMRLSSIDIAEIDD 236
Query: 240 DVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITTDV 299
++ I +++ PHFH+S+QSG D LKRM RR+ + LR +VS D+
Sbjct: 237 ELFELIAYSERIMPHFHISVQSGDDMILKRMKRRHNRASVIEFCQKLRAIRPEVSFGADI 296
Query: 300 IVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSKKLI 359
I GFP + L H+F YS R+GT A M QV N + ER+K L
Sbjct: 297 IAGFPTETNEMFENTRKLILEAELQYLHVFPYSEREGTPAARMP-QVPQNIRKERAKILR 355
Query: 360 E--LNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKV 403
+ N++NE F +++IG+ +++L E +T N+I V
Sbjct: 356 QDGFNQLNE--FFKRHIGQKVELLIENN-------NIAHTENFIPV 392
>MIAB_YERPS RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 474
Score = 192 bits (489), Expect = 4e-51
Identities = 132/428 (30%), Positives = 210/428 (49%), Gaps = 13/428 (3%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVR-EGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S M + GY++ E AD+ ++NTCS+ +K ++
Sbjct: 4 KLHIKTWGCQMNEYDSSKMADLLASTHGYQLTTIPEEADLLLLNTCSIREKAQEKVFSLL 63
Query: 61 GR---ARRQNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
G+ + +NP+ II V GC A ++ VDV+ G + + +N +
Sbjct: 64 GQWKLLKEKNPQLIIGVGGCVASQEGEQLRQRAPCVDVIFGPQTLHRLPEMINHVQGTNS 123
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
V + K F+ L T AF+ I +GCN++C +C++PYTRG S+ +
Sbjct: 124 PVVDISFPEIEK-FDRLPEPRAEGPT-AFVSIMEGCNKYCTFCVVPYTRGEEVSRPSDDI 181
Query: 177 LDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKV-TLVDLLEEIEKIDGIERVRIGSIDP 234
L EI LA G +E+ L G + +Y G D + + +LL + IDGI+R+R + P
Sbjct: 182 LFEIAQLAAQGVREVNLLGQNVNAYRGATYDGDICSFAELLRLVAAIDGIDRIRFTTSHP 241
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
FT+D++ +L HL +QSG D L M R +TA EY+ I+ LR D+
Sbjct: 242 IEFTDDIIDVYRDTPELVSFLHLPVQSGSDRILTMMKRAHTALEYKAIIRKLRQARPDIQ 301
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
I++D IVGFPG + I ++ F YSPR GT A ++ + V +K +R
Sbjct: 302 ISSDFIVGFPGETQQDFEQTMKLVADIHFDTSYSFIYSPRPGTPAADLPDNVSEEEKKQR 361
Query: 355 SKKLIELNRINEKA--FAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVS 412
L + RI+++A + K +G V VL E G T N VN + ++
Sbjct: 362 LHILQQ--RISQQAMEISRKMVGTVQRVLVEGTSRKNVMELAGRTENNRVVNFEGSPDMI 419
Query: 413 GKILNVKI 420
GK ++V+I
Sbjct: 420 GKFVDVEI 427
>MIAB_YERPB RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 474
Score = 192 bits (489), Expect = 4e-51
Identities = 132/428 (30%), Positives = 210/428 (49%), Gaps = 13/428 (3%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVR-EGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S M + GY++ E AD+ ++NTCS+ +K ++
Sbjct: 4 KLHIKTWGCQMNEYDSSKMADLLASTHGYQLTTIPEEADLLLLNTCSIREKAQEKVFSLL 63
Query: 61 GR---ARRQNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
G+ + +NP+ II V GC A ++ VDV+ G + + +N +
Sbjct: 64 GQWKLLKEKNPQLIIGVGGCVASQEGEQLRQRAPCVDVIFGPQTLHRLPEMINHVQGTNS 123
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
V + K F+ L T AF+ I +GCN++C +C++PYTRG S+ +
Sbjct: 124 PVVDISFPEIEK-FDRLPEPRAEGPT-AFVSIMEGCNKYCTFCVVPYTRGEEVSRPSDDI 181
Query: 177 LDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKV-TLVDLLEEIEKIDGIERVRIGSIDP 234
L EI LA G +E+ L G + +Y G D + + +LL + IDGI+R+R + P
Sbjct: 182 LFEIAQLAAQGVREVNLLGQNVNAYRGATYDGDICSFAELLRLVAAIDGIDRIRFTTSHP 241
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
FT+D++ +L HL +QSG D L M R +TA EY+ I+ LR D+
Sbjct: 242 IEFTDDIIDVYRDTPELVSFLHLPVQSGSDRILTMMKRAHTALEYKAIIRKLRQARPDIQ 301
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
I++D IVGFPG + I ++ F YSPR GT A ++ + V +K +R
Sbjct: 302 ISSDFIVGFPGETQQDFEQTMKLVADIHFDTSYSFIYSPRPGTPAADLPDNVSEEEKKQR 361
Query: 355 SKKLIELNRINEKA--FAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVS 412
L + RI+++A + K +G V VL E G T N VN + ++
Sbjct: 362 LHILQQ--RISQQAMEISRKMVGTVQRVLVEGTSRKNVMELAGRTENNRVVNFEGSPDMI 419
Query: 413 GKILNVKI 420
GK ++V+I
Sbjct: 420 GKFVDVEI 427
>MIAB_YERP3 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 474
Score = 192 bits (489), Expect = 4e-51
Identities = 132/428 (30%), Positives = 210/428 (49%), Gaps = 13/428 (3%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVR-EGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S M + GY++ E AD+ ++NTCS+ +K ++
Sbjct: 4 KLHIKTWGCQMNEYDSSKMADLLASTHGYQLTTIPEEADLLLLNTCSIREKAQEKVFSLL 63
Query: 61 GR---ARRQNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
G+ + +NP+ II V GC A ++ VDV+ G + + +N +
Sbjct: 64 GQWKLLKEKNPQLIIGVGGCVASQEGEQLRQRAPCVDVIFGPQTLHRLPEMINHVQGTNS 123
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
V + K F+ L T AF+ I +GCN++C +C++PYTRG S+ +
Sbjct: 124 PVVDISFPEIEK-FDRLPEPRAEGPT-AFVSIMEGCNKYCTFCVVPYTRGEEVSRPSDDI 181
Query: 177 LDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKV-TLVDLLEEIEKIDGIERVRIGSIDP 234
L EI LA G +E+ L G + +Y G D + + +LL + IDGI+R+R + P
Sbjct: 182 LFEIAQLAAQGVREVNLLGQNVNAYRGATYDGDICSFAELLRLVAAIDGIDRIRFTTSHP 241
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
FT+D++ +L HL +QSG D L M R +TA EY+ I+ LR D+
Sbjct: 242 IEFTDDIIDVYRDTPELVSFLHLPVQSGSDRILTMMKRAHTALEYKAIIRKLRQARPDIQ 301
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
I++D IVGFPG + I ++ F YSPR GT A ++ + V +K +R
Sbjct: 302 ISSDFIVGFPGETQQDFEQTMKLVADIHFDTSYSFIYSPRPGTPAADLPDNVSEEEKKQR 361
Query: 355 SKKLIELNRINEKA--FAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVS 412
L + RI+++A + K +G V VL E G T N VN + ++
Sbjct: 362 LHILQQ--RISQQAMEISRKMVGTVQRVLVEGTSRKNVMELAGRTENNRVVNFEGSPDMI 419
Query: 413 GKILNVKI 420
GK ++V+I
Sbjct: 420 GKFVDVEI 427
>MIAB_SPHAL RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 444
Score = 192 bits (489), Expect = 4e-51
Identities = 127/420 (30%), Positives = 212/420 (50%), Gaps = 7/420 (1%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIGRARRQ 66
+ GC++N Y+ E M E EGY + V+NTC + +K IGR +R
Sbjct: 13 SFGCQMNVYDGERMAEMLGAEGYVPXXXXXXXXLVVLNTCHIREKAAEKVYSDIGRLKRD 72
Query: 67 NPEA-IIAVVGCYAQIAPTEVSG-IEGVDVVLGSRNKGEIVYYVNKARDEGKQQVKVGAV 124
+ IAV GC AQ E++ VDVV+G + + + +A + G++ + +
Sbjct: 73 DGSTPTIAVAGCVAQAEGAEIARRAPSVDVVVGPQAYHRLPELIARA-ERGEKAIDLDMP 131
Query: 125 LKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDEIRSLA 184
++K F L N + AFL +Q+GC++FC YC++PYTRG+ S+ ++ E R+L
Sbjct: 132 AESK-FGALPKRSGNQRPTAFLTVQEGCDKFCTYCVVPYTRGAEISRPFADLIAEARALV 190
Query: 185 EHGFKEIILSGIHTASY-GVDLDEKVTLVD-LLEEIEKIDGIERVRIGSIDPTFFTEDVV 242
G +EI L G + ++ G D + +D L+ E+ K DG+ER+R + P T+ ++
Sbjct: 191 AGGVREITLLGQNVNAWIGEDAKGRAIGLDGLIRELAKEDGLERIRYTTSHPNDMTDGLI 250
Query: 243 RRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITTDVIVG 302
+ KL P HL +Q+G D L MNR ++ + Y ++ +R+ D++I+ D IVG
Sbjct: 251 VAHGEVDKLMPFLHLPVQAGSDRILAAMNRSHSVESYLKVIERVREARPDIAISGDFIVG 310
Query: 303 FPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSKKLIELN 362
FPG ++ + + FKYS R GT A M++Q+D +ER ++L L
Sbjct: 311 FPGESEADFAATLDIVRATRYAMAYSFKYSRRPGTPAATMDDQIDEAVMNERLQRLQALL 370
Query: 363 RINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSGKILNVKINS 422
++AF E +G +L E + + G G + V+ A G +++V+I S
Sbjct: 371 NEQQQAFNEATVGRTTRLLLERKGK-REGQLIGKSPWLQSVHVTAPGLAIGDMIDVRITS 429
>MIAB_ACTSZ RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 474
Score = 192 bits (489), Expect = 4e-51
Identities = 141/444 (31%), Positives = 217/444 (48%), Gaps = 18/444 (4%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVR-EGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S + + + G E+ + E ADV ++NTCS+ +K +
Sbjct: 4 KLHIKTWGCQMNEYDSAKIADLLLSTHGLELTEEAEQADVLLLNTCSIREKAQEKVFSQL 63
Query: 61 GRAR---RQNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR + + P II V GC A + VD++ G + + +N+ R GK
Sbjct: 64 GRWKTWKNEKPGLIIGVGGCVASQEGEHIRERAPFVDIIFGPQTLHRLPEMINQIRG-GK 122
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
V + + + F+ L + T AF+ I +GCN++C YC++PYTRG S+ V
Sbjct: 123 SSVVDVSFPEIEKFDCLPEPKAEGPT-AFVSIMEGCNKYCTYCVVPYTRGEEVSRPLDDV 181
Query: 177 LDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKV-TLVDLLEEIEKIDGIERVRIGSIDP 234
L E+ LA+ G +EI L G + +Y G D + T +LL + IDGI+R+R + P
Sbjct: 182 LFEVAQLADQGVREINLLGQNVNAYRGPTHDGGICTFAELLRLVASIDGIDRLRFTTSHP 241
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
FT+D++ +L HL +QSG D L M R +TA EY+ I+ LR D+
Sbjct: 242 IEFTDDIIDVYADTPELVSFLHLPVQSGADRILTMMKRGHTALEYKSIIRKLRKVRPDIQ 301
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
I++D IVGFPG + + + F YS R GT A +M + V +K +R
Sbjct: 302 ISSDFIVGFPGETNEEFEQTMNLIADVNFDMSFSFVYSARPGTPAADMPDDVSEEEKKQR 361
Query: 355 SKKLIELNRINEKA--FAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVS 412
L + RIN +A F+ +G VL E + TG T N VN K ++
Sbjct: 362 LYLLQQ--RINNQAAKFSRAMLGTEQRVLVEGPSKKDIMELTGRTENNRIVNFKGTPDMI 419
Query: 413 GKILNVKI-----NSFEGEVAEGE 431
GK ++++I NS G+V E
Sbjct: 420 GKFVDIQITDVFTNSLRGDVIRTE 443
>RIMO_DESHY RecName: Full=Ribosomal protein S12
methylthiotransferase rimO; Short=S12
methylthiotransferase; Short=S12 MTTase;
Length = 445
Score = 192 bits (488), Expect = 5e-51
Identities = 115/389 (29%), Positives = 201/389 (51%), Gaps = 10/389 (2%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIG 61
KVA TLGC N +SE MT + + Y++V+ E AD+ ++NTC+ +S +I
Sbjct: 7 KVAVVTLGCPKNQVDSEIMTGHMMTK-YQIVNEPEQADIIIINTCTFIESAKAESIDMIL 65
Query: 62 RARRQNPEA---IIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGKQ 117
+ + E + GC AQ E+ + I +D ++G+ N EI+ + +A +
Sbjct: 66 QMSQYKEEGQCQTLVATGCLAQRYGDELLAEIPELDGIMGTGNVAEILETLEEAEKSKVR 125
Query: 118 QV--KVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKK 175
++ + A + ++ +++ K A++K+ +GC+ +C YC+IP+ RG S+ +
Sbjct: 126 RISAEAPAFIYDETMPRVRLSP---KQYAYVKVAEGCDNYCTYCIIPHVRGHFRSRTQES 182
Query: 176 VLDEIRSLAEHGFKEIILSGIHTASYGVDLDEKVTLVDLLEEIEKIDGIERVRIGSIDPT 235
+L E+ ++A G KE++L T YG D + L L++EI +I+GIE +R+ P
Sbjct: 183 ILREVEAMASEGVKEVLLIAQDTTRYGKDRYGEYRLPSLIKEIARIEGIEWIRLMYCYPE 242
Query: 236 FFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSI 295
FT++++ + K+C + L LQ D L MNRR T +E E +++ LR +I D+ +
Sbjct: 243 LFTDELITVMKETPKVCRYLDLPLQHAHDKVLAEMNRRGTIREAEGLIHKLRQEIPDIRL 302
Query: 296 TTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERS 355
T +I GFPG K+I + F YS + T A E+QV + +R
Sbjct: 303 RTTMITGFPGETEEEFQAVVEFAKKIRFDRLGAFAYSQEESTPAAQREDQVPEEIRQQRR 362
Query: 356 KKLIELNRINEKAFAEKYIGEVIDVLFEE 384
+L+EL ++++G+ + VL EE
Sbjct: 363 DQLMELQHDIAYEQQQRWVGQTLKVLIEE 391
>MIAB_STRCO RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 505
Score = 192 bits (488), Expect = 5e-51
Identities = 125/386 (32%), Positives = 185/386 (47%), Gaps = 12/386 (3%)
Query: 7 TLGCRVNTYESEAMTEKFVREGY----EVVDFNEMADVYVVNTCSVTNMGDKKSRQIIGR 62
T GC++N ++SE ++ GY E D + ADV V NTC+V D K +G
Sbjct: 20 TYGCQMNVHDSERLSGLLEDAGYVRAPEGADGD--ADVVVFNTCAVRENADNKLYGNLGH 77
Query: 63 A---RRQNPEAIIAVVGCYAQI-APTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQ 118
+ P IAV GC AQ T V VDVV G+ N G++ + +AR + + Q
Sbjct: 78 LAPKKASRPGMQIAVGGCLAQKDRDTIVKRAPWVDVVFGTHNIGKLPVLLERARVQEEAQ 137
Query: 119 VKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLD 178
V++ L+ F + A++ I GCN C +C++P RG + P +L
Sbjct: 138 VEIAESLE--AFPSTLPTRRESAYAAWVSISVGCNNTCTFCIVPALRGKEKDRRPGDILA 195
Query: 179 EIRSLAEHGFKEIILSGIHTASYGVDLDEKVTLVDLLEEIEKIDGIERVRIGSIDPTFFT 238
E+ +L G EI L G + +YG D+ ++ LL IDG+ERVR S P FT
Sbjct: 196 EVEALVAEGVSEITLLGQNVNAYGSDIGDREAFSKLLRACGNIDGLERVRFTSPHPRDFT 255
Query: 239 EDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITTD 298
+DV+ + P H+ LQSG D LK M R Y + Y I+ +R I +ITTD
Sbjct: 256 DDVIAAMAETPNAMPQLHMPLQSGSDPVLKAMRRSYRQERYLGIIEKVRAAIPHAAITTD 315
Query: 299 VIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSKKL 358
+IVGFPG ++ ++ F+YS R GT A MENQ+ ER ++L
Sbjct: 316 IIVGFPGETEEDFEQTLHVVREARFAQAFTFQYSKRPGTPAAEMENQIPKAVVQERYERL 375
Query: 359 IELNRINEKAFAEKYIGEVIDVLFEE 384
+ L +K +G ++++ E
Sbjct: 376 VALQEEISWDENKKQVGRTLELMVAE 401
>MIAB_RHILO RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 450
Score = 192 bits (488), Expect = 5e-51
Identities = 121/397 (30%), Positives = 197/397 (49%), Gaps = 16/397 (4%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIG 61
KV T GC++N Y+S+ M + +GY D + AD+ ++NTC + +K +G
Sbjct: 8 KVFIKTYGCQMNVYDSQRMGDALAADGYTATDAIDEADLVLLNTCHIREKAAEKVYSELG 67
Query: 62 RARRQNPEAIIA-------VVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARD 113
R R E IA V GC AQ E+ VD+V+G + + + + R
Sbjct: 68 RIRDMKAERAIAGRELLIGVAGCVAQAEGAEIIRRSPAVDLVIGPQTYHRLPDVLARVRG 127
Query: 114 EGKQQVKVGAVLKNKVFEDL----KIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVC 169
G++ V+ +++K F+ L + E AFL +Q+GC++FC +C++PYTRGS
Sbjct: 128 -GEKIVETDYAIEDK-FDHLPQPKRAEVIKRGVTAFLTVQEGCDKFCTFCVVPYTRGSEV 185
Query: 170 SKDPKKVLDEIRSLAEHGFKEIILSGIHTASYGVDLD--EKVTLVDLLEEIEKIDGIERV 227
S+ +++ E LAE G +E+ L G + ++ + E+ L LL + +I G+ R+
Sbjct: 186 SRPVAQIVAEAERLAEAGVREVTLLGQNVNAWHGQGENGEEWGLGRLLFRLAEIPGLARL 245
Query: 228 RIGSIDPTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLR 287
R + P ++++ L L P+ HL +QSG D LK MNRR+TA++Y +++ +R
Sbjct: 246 RYTTSHPRDMDDELISAHRDLPSLMPYLHLPVQSGSDRILKAMNRRHTARDYLALLDRIR 305
Query: 288 DKIEDVSITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVD 347
D++++ D IVGFPG ++++ + FKYSPR GT M + V
Sbjct: 306 AARPDIALSGDFIVGFPGETEADFEATMELVRQVNYASAFSFKYSPRPGTPGAEMPDHVP 365
Query: 348 GNKKDERSKKLIELNRINEKAFAEKYIGEVIDVLFEE 384
KDER F +G ID L E+
Sbjct: 366 ETLKDERXXXXXXXXXXXXXGFGSSLVGSTIDTLIEK 402
>MIAB_PSEA6 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 477
Score = 192 bits (488), Expect = 5e-51
Identities = 138/444 (31%), Positives = 217/444 (48%), Gaps = 18/444 (4%)
Query: 2 KVAFATLGCRVNTYESEAMTEKF-VREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S+ M + GY+V D E AD+ ++NTCS+ +K +
Sbjct: 4 KLFIKTWGCQMNEYDSQKMADLLDSTHGYQVADTAEEADIILLNTCSIREKAQEKVFHQL 63
Query: 61 GR---ARRQNPEAIIAVVGCYA-QIAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR ++ PE II V GC A Q VD+V G + + +N+ +
Sbjct: 64 GRWKNLKQDKPELIIGVGGCVASQEGQVIRQRAPFVDLVFGPQTLHRLPEMINQIKGGSS 123
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
+ + K F+ L + T AF+ I +GC+++C +C++PYTRG S+ V
Sbjct: 124 SVIDISFPEIEK-FDRLPEPKAEGPT-AFVSIMEGCSKYCTFCVVPYTRGEEVSRPVDDV 181
Query: 177 LDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKV-TLVDLLEEIEKIDGIERVRIGSIDP 234
L E+ LAE G +E+ L G + ++ G D + T +LLE + IDGI+R+R + P
Sbjct: 182 LLEVAQLAEQGVREVNLLGQNVNAFRGPHHDGAICTFAELLEMVASIDGIDRIRYTTSHP 241
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
FT+D++ + +L H HL +QSG D L M R +TA EY+ + LR ++S
Sbjct: 242 VEFTDDIIDAYATIPELVDHLHLPVQSGSDRVLNLMKRGHTAIEYKSKIRKLRKIRPNLS 301
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
+++D I+GFPG ++ + + F YS R GT A ++ + V K ER
Sbjct: 302 MSSDFIIGFPGETDDDFEATMDLIQAMDFDLSFSFIYSARPGTPAADLPDDVSETTKKER 361
Query: 355 SKKLIELNRINEKA--FAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVS 412
+ L NRI ++A A I VL E + +G T N VN + ++
Sbjct: 362 LQLL--QNRITQQALRIARNMIDSEQRVLVEGPSKKNPMELSGRTENNRVVNFEGTPDMI 419
Query: 413 GKILNVKI-----NSFEGEVAEGE 431
G ++VKI NS G+V E
Sbjct: 420 GGFVDVKITDVFANSLRGDVLRKE 443
>MIAB_NITSB RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 433
Score = 192 bits (488), Expect = 5e-51
Identities = 140/437 (32%), Positives = 207/437 (47%), Gaps = 23/437 (5%)
Query: 7 TLGCRVNTYESE-AMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIGRARR 65
TLGC +N +SE + E +E YE+ + AD+ ++NTCSV K IG +
Sbjct: 9 TLGCAMNVRDSEHIIAELTQKEDYELTQNLQEADLILINTCSVREKPVHKLFSEIGYFNK 68
Query: 66 QNPE-AIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVKVGA 123
+ E A I V GC A E+ V VLG+RN V+K RD KQ+ V
Sbjct: 69 KKKEGAKIGVCGCTASHLGEEIIKKAPYVSFVLGARN-------VSKIRDVIKQEKAVEV 121
Query: 124 VL----KNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDE 179
+ F D + Y +AF+ I GC++ C +C++P TRG S P+ +L E
Sbjct: 122 DIDYDESTYAFSDFRTSPY----KAFINISIGCDKKCTFCIVPNTRGEEISIPPELILQE 177
Query: 180 IRSLAEHGFKEIILSGIHTASYG---VDLDEKVTLVDLLEEIEKIDGIERVRIGSIDPTF 236
+R + G KEI L G + +YG D KV DLL + +IDG+ R+R S P
Sbjct: 178 VRRAVDTGAKEIFLLGQNVNNYGRSFSDKARKVDFTDLLRMVSEIDGVRRIRFTSPHPLH 237
Query: 237 FTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSIT 296
+ ++ K+C H+ LQSG L+ M R YT + + D LR+ + +V I+
Sbjct: 238 MDDKFLQEFATNPKICKSMHMPLQSGSTKVLRDMKRGYTKEWFLDRAMKLREMVPEVHIS 297
Query: 297 TDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSK 356
TD+IVGFPG ++++ + FKYSPR T A++ ENQ+ R
Sbjct: 298 TDIIVGFPGESEEDFADTIDVVQKVRFEQIFSFKYSPRPLTPAKDYENQIPDEVASRRLS 357
Query: 357 KLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSGKIL 416
L +L+ + +E+ +V +V FEE G G T N V K + G+
Sbjct: 358 YLQDLHLQMLDSISEQEKDKVYEVYFEELKP--GGYVAGRTDNNWIVKVKGSEELLGEFK 415
Query: 417 NVKINSFEGEVAEGEIV 433
+V+I EGE+V
Sbjct: 416 SVRITKPGRLSLEGELV 432
>MIAB_LEPBL RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 449
Score = 192 bits (488), Expect = 5e-51
Identities = 125/442 (28%), Positives = 214/442 (48%), Gaps = 15/442 (3%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKK---SRQ 58
KV T GC++N Y+S ++ Y E +D+ +NTC++ K Q
Sbjct: 12 KVYIETYGCQMNEYDSGIVSSLMKDAEYSSSPDPENSDIIFLNTCAIRENAHAKIYNRLQ 71
Query: 59 IIGRARRQNPEAIIAVVGCYAQIAPTEVSGIE-GVDVVLGSRNKGEIVYYVNKARDEGKQ 117
+G +++NPE +I V+GC AQ ++ E +D+V+G N + + + R G+
Sbjct: 72 SLGYLKKRNPELVIGVLGCMAQNLGDDLFHQELPLDLVVGPDNYRSLPELIQRIRS-GEH 130
Query: 118 QVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVL 177
+ + + K + +++++ N +AF+ I GCN FC +C++PYTRG S+DPK ++
Sbjct: 131 SISLTRLSKIETYDEIEPRVVNG-IQAFVTIMRGCNNFCTFCVVPYTRGRERSRDPKSIV 189
Query: 178 DEIRSLAEHGFKEIILSGIHTASYGVDLDEKVTLVDLLEEIEKIDGIERVRIGSIDPTFF 237
EI+ L E G +++ L G + SY ++ L++ + IER+R S P F
Sbjct: 190 REIQDLTEKGIRQVTLLGQNVNSYK---EQGTDFAGLIQMLLDETTIERIRFTSPHPKDF 246
Query: 238 TEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITT 297
++R + + CP+ HL LQ+G L+ M R Y+ +E+ D+V +R+ + DV ITT
Sbjct: 247 PVRLLRLMAQNPRFCPNIHLPLQAGNTRVLEEMKRSYSKEEFLDVVREIRNIVPDVGITT 306
Query: 298 DVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRA-ENMENQVDGNKKDERSK 356
D+IVGFP ++ + +FKYS R+GT A + + + V K R
Sbjct: 307 DIIVGFPNETEEEFEDTLAVVREVQFDMAFMFKYSEREGTMARKKLPDNVSEETKSARLT 366
Query: 357 KLIELNRINEKAFAEKYIGEVIDVLFE-----EEVELGSGVYTGYTRNYIKVNAKADCNV 411
KL++L IG V +L E E +L G + ++ +
Sbjct: 367 KLVDLQTSISHEQNRARIGRVYSILIENTSRKSEKQLCGRTPCGRMTVFPLPEGRSASEM 426
Query: 412 SGKILNVKINSFEGEVAEGEIV 433
G ++V+I S +G I+
Sbjct: 427 IGSTVSVRIESATSATLKGGIL 448
>MIAB_LEPBJ RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 449
Score = 192 bits (488), Expect = 5e-51
Identities = 125/442 (28%), Positives = 214/442 (48%), Gaps = 15/442 (3%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKK---SRQ 58
KV T GC++N Y+S ++ Y E +D+ +NTC++ K Q
Sbjct: 12 KVYIETYGCQMNEYDSGIVSSLMKDAEYSSSPDPENSDIIFLNTCAIRENAHAKIYNRLQ 71
Query: 59 IIGRARRQNPEAIIAVVGCYAQIAPTEVSGIE-GVDVVLGSRNKGEIVYYVNKARDEGKQ 117
+G +++NPE +I V+GC AQ ++ E +D+V+G N + + + R G+
Sbjct: 72 SLGYLKKRNPELVIGVLGCMAQNLGDDLFHQELPLDLVVGPDNYRSLPELIQRIRS-GEH 130
Query: 118 QVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVL 177
+ + + K + +++++ N +AF+ I GCN FC +C++PYTRG S+DPK ++
Sbjct: 131 SISLTRLSKIETYDEIEPRVVNG-IQAFVTIMRGCNNFCTFCVVPYTRGRERSRDPKSIV 189
Query: 178 DEIRSLAEHGFKEIILSGIHTASYGVDLDEKVTLVDLLEEIEKIDGIERVRIGSIDPTFF 237
EI+ L E G +++ L G + SY ++ L++ + IER+R S P F
Sbjct: 190 REIQDLTEKGIRQVTLLGQNVNSYK---EQGTDFAGLIQMLLDETTIERIRFTSPHPKDF 246
Query: 238 TEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITT 297
++R + + CP+ HL LQ+G L+ M R Y+ +E+ D+V +R+ + DV ITT
Sbjct: 247 PVRLLRLMAQNPRFCPNIHLPLQAGNTRVLEEMKRSYSKEEFLDVVREIRNIVPDVGITT 306
Query: 298 DVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRA-ENMENQVDGNKKDERSK 356
D+IVGFP ++ + +FKYS R+GT A + + + V K R
Sbjct: 307 DIIVGFPNETEEEFEDTLAVVREVQFDMAFMFKYSEREGTMARKKLPDNVSEETKSARLT 366
Query: 357 KLIELNRINEKAFAEKYIGEVIDVLFE-----EEVELGSGVYTGYTRNYIKVNAKADCNV 411
KL++L IG V +L E E +L G + ++ +
Sbjct: 367 KLVDLQTSISHEQNRARIGRVYSILIENTSRKSEKQLCGRTPCGRMTVFPLPEGRSASEM 426
Query: 412 SGKILNVKINSFEGEVAEGEIV 433
G ++V+I S +G I+
Sbjct: 427 IGSTVSVRIESATSATLKGGIL 448
>MIAB_XANCP RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 485
Score = 192 bits (487), Expect = 6e-51
Identities = 136/445 (30%), Positives = 207/445 (46%), Gaps = 14/445 (3%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVR-EGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S M + EG E+ D E ADV +VNTCS+ +K +
Sbjct: 38 KLYIKTHGCQMNEYDSAKMADVLAASEGLELTDNPEDADVVLVNTCSIREKAQEKVFSQL 97
Query: 61 GR---ARRQNPEAIIAVVGCYA-QIAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR + II V GC A Q V VD+V G + + + R+ GK
Sbjct: 98 GRWKALKAGGKPVIIGVGGCVASQEGEAIVKRAPYVDLVFGPQTLHRLPELIRARRESGK 157
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
QV + K D E + AF+ I +GC+++C++C++PYTRG S+ + V
Sbjct: 158 SQVDISFPEIEKF--DRLPEPRADGPSAFVSIMEGCSKYCSFCVVPYTRGEEVSRPFEDV 215
Query: 177 LDEIRSLAEHGFKEIILSG----IHTASYGVDLDEKVTLVDL---LEEIEKIDGIERVRI 229
L E+ LA G +EI L G + +YG D E DL + I +IDGI R+R
Sbjct: 216 LVEVAQLAAQGVREINLLGQNVNAYRGAYGADAGEPAQYADLGLLIRTIAQIDGIGRIRF 275
Query: 230 GSIDPTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDK 289
+ P F++ +V + +L + HL +Q+G D L M R YTA E++ + LR
Sbjct: 276 TTSHPLEFSDSLVDAYRDVPQLANYLHLPVQAGSDRILSAMKRGYTALEFKSKIRKLRAV 335
Query: 290 IEDVSITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGN 349
D+SI++D IVGFPG ++ + ++ F YS R GT A ++E+
Sbjct: 336 RPDISISSDFIVGFPGETDADFDKTMKLIEDVGFDQSFSFIYSRRPGTPASDLEDDTPDA 395
Query: 350 KKDERSKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADC 409
K R +L + +++ +G V VL E + TG T N VN +
Sbjct: 396 VKQARLARLQAHINAHAAGISQRMVGSVQRVLVEGPSRRDANELTGKTENMRPVNFPGNP 455
Query: 410 NVSGKILNVKINSFEGEVAEGEIVL 434
+ G+ ++V I G I L
Sbjct: 456 RLVGQFVDVLITEALSNSLRGRIQL 480
>MIAB_XANCB RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 485
Score = 192 bits (487), Expect = 6e-51
Identities = 136/445 (30%), Positives = 207/445 (46%), Gaps = 14/445 (3%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVR-EGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S M + EG E+ D E ADV +VNTCS+ +K +
Sbjct: 38 KLYIKTHGCQMNEYDSAKMADVLAASEGLELTDNPEDADVVLVNTCSIREKAQEKVFSQL 97
Query: 61 GR---ARRQNPEAIIAVVGCYA-QIAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR + II V GC A Q V VD+V G + + + R+ GK
Sbjct: 98 GRWKALKAGGKPVIIGVGGCVASQEGEAIVKRAPYVDLVFGPQTLHRLPELIRARRESGK 157
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
QV + K D E + AF+ I +GC+++C++C++PYTRG S+ + V
Sbjct: 158 SQVDISFPEIEKF--DRLPEPRADGPSAFVSIMEGCSKYCSFCVVPYTRGEEVSRPFEDV 215
Query: 177 LDEIRSLAEHGFKEIILSG----IHTASYGVDLDEKVTLVDL---LEEIEKIDGIERVRI 229
L E+ LA G +EI L G + +YG D E DL + I +IDGI R+R
Sbjct: 216 LVEVAQLAAQGVREINLLGQNVNAYRGAYGADAGEPAQYADLGLLIRTIAQIDGIGRIRF 275
Query: 230 GSIDPTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDK 289
+ P F++ +V + +L + HL +Q+G D L M R YTA E++ + LR
Sbjct: 276 TTSHPLEFSDSLVDAYRDVPQLANYLHLPVQAGSDRILSAMKRGYTALEFKSKIRKLRAV 335
Query: 290 IEDVSITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGN 349
D+SI++D IVGFPG ++ + ++ F YS R GT A ++E+
Sbjct: 336 RPDISISSDFIVGFPGETDADFDKTMKLIEDVGFDQSFSFIYSRRPGTPASDLEDDTPDA 395
Query: 350 KKDERSKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADC 409
K R +L + +++ +G V VL E + TG T N VN +
Sbjct: 396 VKQARLARLQAHINAHAAGISQRMVGSVQRVLVEGPSRRDANELTGKTENMRPVNFPGNP 455
Query: 410 NVSGKILNVKINSFEGEVAEGEIVL 434
+ G+ ++V I G I L
Sbjct: 456 RLVGQFVDVLITEALSNSLRGRIQL 480
>MIAB_XANC8 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 485
Score = 192 bits (487), Expect = 6e-51
Identities = 136/445 (30%), Positives = 207/445 (46%), Gaps = 14/445 (3%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVR-EGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S M + EG E+ D E ADV +VNTCS+ +K +
Sbjct: 38 KLYIKTHGCQMNEYDSAKMADVLAASEGLELTDNPEDADVVLVNTCSIREKAQEKVFSQL 97
Query: 61 GR---ARRQNPEAIIAVVGCYA-QIAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR + II V GC A Q V VD+V G + + + R+ GK
Sbjct: 98 GRWKALKAGGKPVIIGVGGCVASQEGEAIVKRAPYVDLVFGPQTLHRLPELIRARRESGK 157
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
QV + K D E + AF+ I +GC+++C++C++PYTRG S+ + V
Sbjct: 158 SQVDISFPEIEKF--DRLPEPRADGPSAFVSIMEGCSKYCSFCVVPYTRGEEVSRPFEDV 215
Query: 177 LDEIRSLAEHGFKEIILSG----IHTASYGVDLDEKVTLVDL---LEEIEKIDGIERVRI 229
L E+ LA G +EI L G + +YG D E DL + I +IDGI R+R
Sbjct: 216 LVEVAQLAAQGVREINLLGQNVNAYRGAYGADAGEPAQYADLGLLIRTIAQIDGIGRIRF 275
Query: 230 GSIDPTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDK 289
+ P F++ +V + +L + HL +Q+G D L M R YTA E++ + LR
Sbjct: 276 TTSHPLEFSDSLVDAYRDVPQLANYLHLPVQAGSDRILSAMKRGYTALEFKSKIRKLRAV 335
Query: 290 IEDVSITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGN 349
D+SI++D IVGFPG ++ + ++ F YS R GT A ++E+
Sbjct: 336 RPDISISSDFIVGFPGETDADFDKTMKLIEDVGFDQSFSFIYSRRPGTPASDLEDDTPDA 395
Query: 350 KKDERSKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADC 409
K R +L + +++ +G V VL E + TG T N VN +
Sbjct: 396 VKQARLARLQAHINAHAAGISQRMVGSVQRVLVEGPSRRDANELTGKTENMRPVNFPGNP 455
Query: 410 NVSGKILNVKINSFEGEVAEGEIVL 434
+ G+ ++V I G I L
Sbjct: 456 RLVGQFVDVLITEALSNSLRGRIQL 480
>MIAB_SHELP RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 474
Score = 192 bits (487), Expect = 6e-51
Identities = 136/444 (30%), Positives = 217/444 (48%), Gaps = 18/444 (4%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVR-EGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S M + +GY + + E ADV ++NTCS+ +K +
Sbjct: 4 KLHIKTWGCQMNEYDSSKMADLLDEYQGYTLTEEAEEADVLLLNTCSIREKAQEKVFHQL 63
Query: 61 GR---ARRQNPEAIIAVVGCYAQIAPTEVSG-IEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR + +NP II V GC A + + VD++ G + + + + + GK
Sbjct: 64 GRWKTLKDKNPNLIIGVGGCVASQEGKAIKDRAQCVDLIFGPQTLHRLPEMIEQIQQGGK 123
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
+ V K D E AF+ I +GC+++C++C++PYTRG S+ V
Sbjct: 124 AVIDVSFPEIEKF--DRLPEPRAEGPSAFVSIMEGCSKYCSFCVVPYTRGEEVSRPVDDV 181
Query: 177 LDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKV-TLVDLLEEIEKIDGIERVRIGSIDP 234
+ EI LAE G +E+ L G + +Y G D+ + T +LL + IDGI+R+R + P
Sbjct: 182 ILEIAQLAEQGVREVNLLGQNVNAYRGATHDDDICTFAELLRYVAAIDGIDRIRFTTSHP 241
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
FT+D++ +L HL +QSG D L +M R + A EY+ I+ LR D+
Sbjct: 242 IEFTQDIIDVYEDTPELVSFLHLPVQSGSDLILTQMKRGHMAIEYKSIIRRLRKARPDIQ 301
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
I++D IVGFPG ++ + + F YS R GT A ++ + V ++K ER
Sbjct: 302 ISSDFIVGFPGESKQDFADTMKLIEDVQFDHSFSFIYSARPGTPASDLPDDVSLDEKKER 361
Query: 355 SKKLIELNRINEKA--FAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVS 412
I +RI ++A ++ + +G V +L E G T N VN +AD
Sbjct: 362 --LAILQDRITQQAMRYSRQMLGTVQRILVEGPSVKNPMELRGRTENNRVVNFEADPKHI 419
Query: 413 GKILNVKI-----NSFEGEVAEGE 431
G ++V+I NS G+ GE
Sbjct: 420 GSFVDVEIVDVFTNSLRGKFIRGE 443
>MIAB_RALPJ RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 456
Score = 192 bits (487), Expect = 6e-51
Identities = 145/448 (32%), Positives = 209/448 (46%), Gaps = 22/448 (4%)
Query: 2 KVAFATLGCRVNTYESEAMTEKF-VREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
KV T GC++N Y+S+ M++ EG D E ADV + NTCSV +K +
Sbjct: 3 KVFIKTYGCQMNEYDSDKMSDVLNAAEGLVPTDTPEDADVILFNTCSVREKAQEKVFSEL 62
Query: 61 GRARRQN---PEAIIAVVGCYA-QIAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR + P+ ++ V GC A Q + V+ VDVV G + + + R G+
Sbjct: 63 GRVKALKALKPDLVVGVGGCVASQEGASIVARAPYVDVVFGPQTLHRLPDLIAARRRTGR 122
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
QV V K F+ L + + A++ I +GC+++C+YC++PYTRG S+ V
Sbjct: 123 SQVDVSFPEIEK-FDHLPPARVDGAS-AYVSIMEGCSKYCSYCVVPYTRGEEVSRPFDDV 180
Query: 177 LDEIRSLAEHGFKEIILSGIHTASY---GVDLDEKVTLVDLLEEIEKIDGIERVRIGSID 233
L E+ LAE G +E+ L G + +Y D E+ LLE + +I GIER+R +
Sbjct: 181 LAEVAGLAEQGVREVTLLGQNVNAYIGKMGDTSERADFALLLEYVAEIPGIERIRYTTSH 240
Query: 234 PTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDV 293
P F+ ++ KL H HL +Q G D L M R YT EY+ + LR ++
Sbjct: 241 PKEFSSRLIEAYATNPKLVDHLHLPVQHGSDRILMAMKRGYTVLEYKSSIRKLRAIRPNI 300
Query: 294 SITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDE 353
SI TD IVGFPG + I + F YSPR GT A N+ + K E
Sbjct: 301 SIATDFIVGFPGETDADFAKTMDLIHEIGYDTSFSFIYSPRPGTPAANLHDDTPQAVKLE 360
Query: 354 RSKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVN-AKADC--- 409
R K L N ++ +G V +L E G T N VN A D
Sbjct: 361 RLKHLQATIEENVARISQGMVGSVQRILVEGPSRKDPTELHGRTENNRVVNFALPDLPQV 420
Query: 410 ---NVSGKILNVKI-----NSFEGEVAE 429
+ G++L+V+I +S GEVAE
Sbjct: 421 RREQLIGQMLDVRIVHAFPHSLRGEVAE 448
>MIAB_MANSM RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 474
Score = 192 bits (487), Expect = 6e-51
Identities = 137/444 (30%), Positives = 222/444 (50%), Gaps = 18/444 (4%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVR-EGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S M + G E+ + E ADV ++NTCS+ +K +
Sbjct: 4 KLHIKTWGCQMNEYDSSKMADLLQSTHGLELTEEAEQADVLLLNTCSIREKAQEKVFHQL 63
Query: 61 GR---ARRQNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR +++NP +I V GC A + VD++ G + + +N+ R G+
Sbjct: 64 GRWKELKKKNPNLVIGVGGCVASQEGEHIRERAPYVDIIFGPQTLHRLPEMINQIR-AGE 122
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
+ V + + + F+ L + T AF+ I +GCN++C YC++PYTRG S+ V
Sbjct: 123 KAVLDISFPEIEKFDRLPEPKAEGPT-AFVSIMEGCNKYCTYCVVPYTRGEEVSRPLDDV 181
Query: 177 LDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKV-TLVDLLEEIEKIDGIERVRIGSIDP 234
L EI LAE G +E+ L G + +Y G D + + +LL + IDGI+R+R + +P
Sbjct: 182 LFEIAQLAEQGVREVNLLGQNVNAYRGPTHDGGICSFAELLRLVAAIDGIDRLRFTTSNP 241
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
FT+D++ +L HL +Q+G D L M R +TA EY+ I+ LR ++
Sbjct: 242 IEFTDDIIDVYRDTPELVSFLHLPVQAGSDRILTMMKRGHTAIEYKSIIRKLRAVRPNIQ 301
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
I++D IVGFPG ++++ + F YS R GT A +M + V +++++
Sbjct: 302 ISSDFIVGFPGETNEEFEQTMNLIQQVNFDMSFSFVYSARPGTPAADMPDDV--TEEEKK 359
Query: 355 SKKLIELNRINEKA--FAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVS 412
+ I RIN +A F+ +G VL E + TG T N VN ++
Sbjct: 360 QRLYILQQRINNQAAQFSRAMLGTEQRVLVEGPSKKDIMELTGRTENNRIVNFAGTPDMI 419
Query: 413 GKILNVKI-----NSFEGEVAEGE 431
GK +++KI NS G+V E
Sbjct: 420 GKFVDIKITDVFTNSLRGDVVRTE 443
>MIAB_LEPCP RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 469
Score = 192 bits (487), Expect = 6e-51
Identities = 136/442 (30%), Positives = 207/442 (46%), Gaps = 11/442 (2%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVR-EGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
KV T GC++N Y+S+ M++ EGY D E AD+ + NTCSV +K +
Sbjct: 28 KVYIRTFGCQMNEYDSDKMSDVMAAAEGYTPTDDPEQADLILFNTCSVREKAQEKVFSDL 87
Query: 61 GRARRQNPEAI-IAVVGCYA-QIAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQ 118
GR + + I V GC A Q + VDVV G + + + + +G+ Q
Sbjct: 88 GRVKHLKARGVLIGVGGCVASQEGAAIIERAPYVDVVFGPQTLHRLPQMLAQRARQGRPQ 147
Query: 119 VKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLD 178
V + K F+ L + + AF+ I +GC+++C+YC++PYTRG S+ +VL
Sbjct: 148 VDISFPEIEK-FDHLPPAKVDGAA-AFVSIMEGCSKYCSYCVVPYTRGEEFSRPFDEVLT 205
Query: 179 EIRSLAEHGFKEIILSGIHTASYGVDLDEKVTLVD---LLEEIEKIDGIERVRIGSIDPT 235
E+ LA+ G KE+ L G + +Y + + + D LLE + +I GIER+R + P
Sbjct: 206 EVAGLADQGVKEVTLLGQNVNAYRGRMGDTSDIADFATLLEYVHEIPGIERIRYTTSHPN 265
Query: 236 FFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSI 295
FT ++ L KL H HL +Q G D L M R YT EY+ + LR D+S+
Sbjct: 266 EFTPALIEAYARLPKLVNHLHLPVQHGSDRILSGMKRGYTVLEYKSTIRKLRAIRPDISL 325
Query: 296 TTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERS 355
+TD IVGFPG + I + F +SPR GT A + + K R
Sbjct: 326 STDFIVGFPGETDDDHARTMKLIDDIGFDASFSFIFSPRPGTPAAALHDDTSYEVKLARL 385
Query: 356 KKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVS--- 412
++L N + +E+ +G V VL E G T VN N +
Sbjct: 386 QQLQATIEDNVRRISERRVGTVQRVLVEGPSRRDPNELMGRTECNRIVNFDGGPNAARLV 445
Query: 413 GKILNVKINSFEGEVAEGEIVL 434
G++++++I GE VL
Sbjct: 446 GRMIDIRITLAYPHSLRGEPVL 467
>MIAB_ACIBL RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 444
Score = 192 bits (487), Expect = 6e-51
Identities = 135/385 (35%), Positives = 205/385 (53%), Gaps = 11/385 (2%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIGRARRQ 66
T GC++N ++SE + + +GY V+ A + + NTCS+ + ++K + R+
Sbjct: 14 TFGCQMNFHDSEKVVGTLISQGYRQVETELDAGLILYNTCSIRDKAEQKVFHRLSEFRQL 73
Query: 67 NPEAI-IAVVGCYAQIAPTEV-SGIEGVDVVLGS---RNKGEIVYYVNKARDEGKQQVKV 121
E AV+GC AQ ++ V +V GS RN E++ + + G Q++
Sbjct: 74 QKEGKRFAVLGCVAQQEGEKIFERAPHVSLVAGSASYRNLAEMLVQI----ESGSQRITG 129
Query: 122 GAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDEIR 181
+ + + N R ++ I +GC++FCAYC++PYTRG S+ + VL E R
Sbjct: 130 LDDRETDQTFETEFTARGNAHRGYITIIEGCDKFCAYCVVPYTRGKERSRSAESVLREAR 189
Query: 182 SLAEHGFKEIILSGIHTASYGVDLDEKVTLVDLLEEIEKIDGIERVRIGSIDPTFFTEDV 241
+A+ GF ++ L G + SY D +T +LL + +I GI+RVR + P FT D+
Sbjct: 190 QMADAGFTDVQLLGQNVNSYH-DPSGTMTFAELLTAVGEITGIKRVRFTTSHPRDFTRDI 248
Query: 242 VRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLR-DKIEDVSITTDVI 300
V I LC H HL +QSG L+ M R YT ++Y + ++ ++ K +SITTDVI
Sbjct: 249 VEAIDNHPTLCDHVHLPVQSGSSKVLREMFREYTREQYLERISWMKATKNRKLSITTDVI 308
Query: 301 VGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSKKLIE 360
VGFPG L + FKYSPR T A + V +K R + L+E
Sbjct: 309 VGFPGXXXXXXXXXLALLDHVQYDGVFSFKYSPRPNTPALKYIDTVPEQEKSRRLQILME 368
Query: 361 LNRINEKAFAEKYIGEVIDVLFEEE 385
R ++A K+IGE I+V+ E E
Sbjct: 369 HQREIQRANYRKHIGETIEVMVEAE 393
>MIAB_NITWN RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 477
Score = 191 bits (486), Expect = 8e-51
Identities = 130/435 (29%), Positives = 210/435 (48%), Gaps = 13/435 (2%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIG 61
K+ + GC++N Y+++ M + EGY E AD+ ++NTC + +K +G
Sbjct: 10 KLHIKSYGCQMNVYDAQRMADTLAVEGYVETTSAEDADLVILNTCHIREKASEKVYSELG 69
Query: 62 RARRQNPEAI-------IAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARD 113
R R +A I V GC AQ E+ VDVV+G ++ + + +A+
Sbjct: 70 RLRAAKDQAARDGRGMNIVVAGCVAQAEGEEIIRRAPMVDVVVGPQSYHHLPQLLARAKT 129
Query: 114 EGKQQVKVGAVLKNKVF--EDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSK 171
G+ V F + + + AF+ +Q+GC++FC +C++PYTRG+ S+
Sbjct: 130 CGRALETEFPVADKFGFLPQPSRQAIRSRGISAFVTVQEGCDKFCTFCVVPYTRGAEASR 189
Query: 172 DPKKVLDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKV-TLVDLLEEIEKIDGIERVRI 229
KV++++R LA++G +EI L G + +Y G D + LL + I GI R+R
Sbjct: 190 PVAKVVEDVRRLADNGVREITLIGQNVNAYHGEGPDGRPWPFGKLLYRLAGIPGIVRLRY 249
Query: 230 GSIDPTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDK 289
+ P + ++ L L P HL +QSG D L MNRR+TA +Y +++ R
Sbjct: 250 STSHPRDVEDTLIDAHRDLDALMPFVHLPVQSGSDRILAAMNRRHTADDYRRVIDRFRQA 309
Query: 290 IEDVSITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGN 349
D++ ++D IVGFPG + +I + + FKYSPR GT A +M+ V
Sbjct: 310 RPDIAFSSDFIVGFPGETEEDFAATLALVGQIGYAAAYSFKYSPRPGTPAADMQETVSTA 369
Query: 350 KKDERSKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADC 409
D+R +L L + AF +G IDVLF + G G T + +A
Sbjct: 370 DMDQRLVQLQNLIDSQQSAFNRTALGRTIDVLF-DRAGRKPGQIVGRTAYLQPAHVEASD 428
Query: 410 NVSGKILNVKINSFE 424
+ G++L V + S E
Sbjct: 429 AIIGQVLPVTVASLE 443
>MIAB_AZOC5 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 464
Score = 191 bits (486), Expect = 8e-51
Identities = 131/442 (29%), Positives = 222/442 (50%), Gaps = 15/442 (3%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIG 61
K+ + GC++N Y+S+ M + +EGY AD+ ++NTC + +K +G
Sbjct: 6 KLYVKSFGCQMNVYDSQRMADALAKEGYVETQDPADADLVILNTCHIREKAAEKVYSELG 65
Query: 62 RARRQNPEA----IIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
R R+ +A IAV GC AQ E+ VD+V+G ++ + + + RD GK
Sbjct: 66 RLRKAKQDAGSDTTIAVAGCVAQAEGAEIMKRAPVVDLVVGPQSYHRLPEMLARVRD-GK 124
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTR---AFLKIQDGCNRFCAYCLIPYTRGSVCSKDP 173
+ V ++K K R AF+ +Q+GC++FC +C++PYTRG+ S+
Sbjct: 125 RVVDTEFPAEDKFDHLPAPSAAATKKRGPTAFVTVQEGCDKFCTFCVVPYTRGAEVSRSV 184
Query: 174 KKVLDEIRSLAEHGFKEIILSGIHT-ASYGVDLDEKVT-LVDLLEEIEKIDGIERVRIGS 231
K++ E R+L + G +EI L G + A +G D + L LLEE+ I+G+ R+R +
Sbjct: 185 SKIVGEARALVDQGVREITLIGQNVNAFHGEGPDGRTWGLGRLLEELAGINGLARLRYTT 244
Query: 232 IDPTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIE 291
P + ++ L +L P+ HL +QSG D L MNR++ + Y +I++ LR
Sbjct: 245 SHPRDMDDGLIAAHRDLPQLMPYLHLPVQSGSDRILAAMNRKHGREIYFEIIDKLRAARP 304
Query: 292 DVSITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKK 351
D+++++D IVGFPG +R+ + + FKYS R GT A ++Q+ K
Sbjct: 305 DIALSSDFIVGFPGETEADFEDTLDLARRVGFASAYSFKYSIRPGTPAAEHDHQLSEEVK 364
Query: 352 DERSKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVY--TGYTRNYIKVNAKADC 409
ER +L ++ ++ AF +G D+L E+ L + + Y ++ + A
Sbjct: 365 SERLARLHQVLEASKTAFDRACMGRRFDILLEKPGRLPGQLIGRSPYLQSVVVTAPGAGI 424
Query: 410 N--VSGKILNVKINSFEGEVAE 429
V+ I +V NS G+V +
Sbjct: 425 GDFVTVDITDVGPNSLAGQVVD 446
>RIMO_CLOD6 RecName: Full=Ribosomal protein S12
methylthiotransferase rimO; Short=S12
methylthiotransferase; Short=S12 MTTase;
Length = 444
Score = 191 bits (485), Expect = 1e-50
Identities = 131/432 (30%), Positives = 217/432 (50%), Gaps = 16/432 (3%)
Query: 1 MKVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
+K+A +LGC N ++E M +GY+++ E ADV +VNTC ++S I
Sbjct: 2 LKIALESLGCSKNLVDAEIMMGILNNKGYKLIGDFEEADVIIVNTCGFIESAKQESIDTI 61
Query: 61 ---GRARRQNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
++ ++ V GC AQ E+ + I +D ++G+ + I + K E
Sbjct: 62 INFAELKKTGNLKLLIVTGCLAQRYSEELKTEIPEIDAIVGTGSYQNIDKIL-KELSEIH 120
Query: 117 QQVKVGAVLKNKVF-EDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKK 175
Q V + + VF EDL A+LKI +GC+ C YC+IP RG S+ +
Sbjct: 121 QIVSLNDI--EFVFNEDLPRYISTPSYMAYLKIGEGCSNNCTYCIIPKLRGKYRSRKFED 178
Query: 176 VLDEIRSLAEHGFKEIILSGIHTASYGVDLDEKVTLVDLLEEIEKIDGIERVRIGSIDPT 235
++ E + LAE G KE+++ T YG DL K L +LLEE+ KIDG + +R+ P
Sbjct: 179 IIKEAKKLAESGVKELVVIAQDTTKYGFDLYGKERLSELLEELAKIDGFKWIRVMYSYPE 238
Query: 236 FFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSI 295
TE++++ I +C +F + +Q + LK MNR+ T ++ + +NL+R I D +
Sbjct: 239 SITEELIQVIKKYDNICSYFDMPIQHASNNILKLMNRKTTKEDILNKINLIRSNIPDAIL 298
Query: 296 TTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERS 355
T +IVGFPG ++ + + F YS + T A+ + N +D K +R
Sbjct: 299 RTTIIVGFPGETEDDFKQLVDFVEEVKFDRLGAFAYSREEDTPADRLPNHIDEEVKIQRR 358
Query: 356 KKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIK-----VNAKADCN 410
L+ + + + +K IG+ +VL EE++E VYTG T+ + V K+ N
Sbjct: 359 DTLMMIQQKISEELNDKKIGKTYEVLIEEQIE--DNVYTGRTQGDAEEIDSIVYVKSVDN 416
Query: 411 VS-GKILNVKIN 421
+ G+ ++V+IN
Sbjct: 417 LEVGEFVSVQIN 428
>MIAB_ZYMMO RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 445
Score = 191 bits (485), Expect = 1e-50
Identities = 117/345 (33%), Positives = 188/345 (54%), Gaps = 8/345 (2%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIGRARRQ 66
+ GC++N+Y+ E M+E +G + + AD+ V+NTC + +K IGR RR
Sbjct: 13 SFGCQMNSYDGERMSELLESQGMKAAEEAATADLVVLNTCHIREKAAEKVYSEIGRLRRP 72
Query: 67 NPEA-IIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVKVGAV 124
+ + +IA+ GC AQ EV + + VD+V+G + + + KA GK V +
Sbjct: 73 DGSSPMIALAGCVAQAEGAEVLARTKMVDIVVGPQAYHHLPELIEKAAS-GKV-VDIDMP 130
Query: 125 LKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDEIRSLA 184
L++K D E AFL +Q+GC++FC YC++PYTRG+ S+ +++ E +L
Sbjct: 131 LESKF--DALPERRQVGASAFLTVQEGCDKFCTYCVVPYTRGAEVSRPWSRIVKEAHALV 188
Query: 185 EHGFKEIILSGIHTASY-GVDLDEKVTLVD-LLEEIEKIDGIERVRIGSIDPTFFTEDVV 242
+ G +EI L G + ++ G D + +D L+ + KIDG+ER+R + P TE ++
Sbjct: 189 DKGAREITLLGQNVNAWTGEDEAGRSQGLDGLIRALAKIDGLERIRYTTSHPNDMTEGLI 248
Query: 243 RRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITTDVIVG 302
+ KL P HL +QSG + LK MNR +TA+ Y ++N L++ D++++ D IVG
Sbjct: 249 EAHGEIDKLMPFLHLPVQSGSNRILKAMNRAHTAESYLTLMNRLKEVRPDIALSGDFIVG 308
Query: 303 FPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVD 347
FPG + + S F YSPR GT A +M+NQ+D
Sbjct: 309 FPGESEEDFQATLDLISEVGYSLAFSFAYSPRPGTPAADMDNQID 353
>MIAB_CORU7 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 540
Score = 191 bits (485), Expect = 1e-50
Identities = 117/388 (30%), Positives = 197/388 (50%), Gaps = 12/388 (3%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKK---SRQIIGRA 63
T GC++N ++SE ++ GY+ E DV V NTC+V D + + ++
Sbjct: 47 TFGCQMNVHDSERLSGLLEDSGYQPAANGEEPDVLVFNTCAVRENADNRLYGTLAMVKPM 106
Query: 64 RRQNPEAIIAVVGCYAQI-APTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVKVG 122
+ +NP IAV GC AQ V VDVV G+ N G + + ++ + +V++
Sbjct: 107 KDRNPGMQIAVGGCMAQKDKDAVVDRAPWVDVVFGTHNIGSLPTLLERSAHNQRAEVEIL 166
Query: 123 AVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDEIRS 182
L+ F + + + ++ + GCN C +C++P RG + P ++L E+++
Sbjct: 167 DSLEE--FPSVLPAKRESAYSGWVSVSVGCNNTCTFCIVPSLRGKEQDRRPGEILAEVQA 224
Query: 183 LAEHGFKEIILSGIHTASYGVDL-DEKV-----TLVDLLEEIEKIDGIERVRIGSIDPTF 236
L + G +E+ L G + +YGV+ DE + LL +I+G+ER+R S P
Sbjct: 225 LVDQGVQEVTLLGQNVNAYGVNFADEDLPRDRGAFAKLLRACGEIEGLERLRFTSPHPAE 284
Query: 237 FTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSIT 296
FT+DV+ + +CP H+ LQSG D LK M R Y +K++ I++ +R++I +IT
Sbjct: 285 FTDDVIDAMAETPNVCPQLHMPLQSGSDRILKEMRRSYRSKKFLGILDKVRERIPHAAIT 344
Query: 297 TDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSK 356
TD+IVGFPG +++ S F+YSPR GT A M +Q+ ER +
Sbjct: 345 TDIIVGFPGETEEDFQATLDVVEKARFSCAFTFQYSPRPGTPAATMPDQIPKAVVQERYE 404
Query: 357 KLIELNRINEKAFAEKYIGEVIDVLFEE 384
+LI L + ++ +G ++L +E
Sbjct: 405 RLIALQERIQAEDNKELVGTTQELLVQE 432
>MIAB_XANP2 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 470
Score = 191 bits (484), Expect = 1e-50
Identities = 119/394 (30%), Positives = 201/394 (51%), Gaps = 13/394 (3%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIG 61
K+ + GC++N Y+S M + REG+ AD+ ++NTC + +K +G
Sbjct: 6 KLYVKSFGCQMNVYDSHRMADTLAREGFVETQDPAEADLVILNTCHIREKAAEKVYSELG 65
Query: 62 RARRQNPEA----IIAVVGCYAQIAPTEVSG-IEGVDVVLGSRNKGEIVYYVNKARDEGK 116
R R+Q EA +IAV GC AQ E+ VD+V+G ++ + + +A+ GK
Sbjct: 66 RLRKQKQEAGSDTMIAVAGCVAQAEGAEIMRRAPVVDLVVGPQSYHRLPELLAEAK-AGK 124
Query: 117 QQVKVGAVLKNKVFEDL----KIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKD 172
+ V ++K F+ L + + AF+ +Q+GC++FC +C++PYTRG+ S+
Sbjct: 125 RVVDTEFPAEDK-FDHLPAPTRAATRSRGPAAFVTVQEGCDKFCTFCVVPYTRGAEVSRP 183
Query: 173 PKKVLDEIRSLAEHGFKEIILSGIHT-ASYGVDLDEKV-TLVDLLEEIEKIDGIERVRIG 230
K++DE L + G +EI L G + A +G D +L L+E + ++DG+ R+R
Sbjct: 184 VAKIMDEAARLVDQGVREISLIGQNVNAFHGAGPDGTTWSLARLMEHMARLDGLARLRYT 243
Query: 231 SIDPTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKI 290
+ P +D++ L +L P+ HL +QSG D L MNR++ ++ I+ +R
Sbjct: 244 TSHPRDMDDDLIAAHRDLPQLMPYLHLPVQSGSDRILAAMNRKHGRDDFFAIIEKMRQAR 303
Query: 291 EDVSITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNK 350
D+++++D IVGFPG ++R+ + + FKYSPR GT A + + QV
Sbjct: 304 PDMALSSDFIVGFPGESEKDFEDTLDLVRRVGFASAYSFKYSPRPGTPAADHDAQVPEEV 363
Query: 351 KDERSKKLIELNRINEKAFAEKYIGEVIDVLFEE 384
K ++ AF E G D+L E+
Sbjct: 364 KXXXXXXXXXXXXXSKAAFDESCRGRTFDILLEK 397
>MIAB_RHOPB RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 473
Score = 191 bits (484), Expect = 1e-50
Identities = 132/445 (29%), Positives = 213/445 (47%), Gaps = 17/445 (3%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIG 61
K+ + GC++N Y+++ M + EG+ + AD+ ++NTC + +K +G
Sbjct: 6 KLHIKSFGCQMNVYDAQRMVDALAPEGFVETANADEADLVILNTCHIREKASEKVYSELG 65
Query: 62 RARRQNPEAI-------IAVVGCYAQIAPTEVSGIE-GVDVVLGSRNKGEIVYYVNKARD 113
R R EA IAV GC AQ E+ + VDVV+G ++ +
Sbjct: 66 RLRVAKDEAALQGRRMNIAVAGCVAQAEGAEIIRRQPAVDVVVGPQSYHHLPQXXXXXXX 125
Query: 114 EGKQQVKVGAVLKNKVFEDLKIEEYN----NKTRAFLKIQDGCNRFCAYCLIPYTRGSVC 169
V + F L + + AF+ +Q+GC++FC +C++PYTRG+
Sbjct: 126 XXXXXXTEFPV--DDKFGFLPPPQPDAIRARGISAFVTVQEGCDKFCTFCVVPYTRGAEV 183
Query: 170 SKDPKKVLDEIRSLAEHGFKEIILSGIHTASYGVDLDE--KVTLVDLLEEIEKIDGIERV 227
S+ K+++++R LA++G +EI L G + +Y D + L LL + KI GI R+
Sbjct: 184 SRPVDKIVEDVRRLADNGVREITLIGQNVNAYHGDGPDGRPWPLGALLHHLAKIPGIVRL 243
Query: 228 RIGSIDPTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLR 287
R + P + ++ L L P HL +QSG DA L MNR+++A +Y +V+ R
Sbjct: 244 RYSTSHPRDVDQSLIDAHRDLPALMPFVHLPVQSGSDAILAAMNRKHSADDYRRLVDRFR 303
Query: 288 DKIEDVSITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVD 347
++ ++D IVGFPG + +I + + FKYSPR GT A ++ V
Sbjct: 304 SANPAIAFSSDFIVGFPGETDEDFAATLALVTQIGYAGAYSFKYSPRPGTPAAELQETVS 363
Query: 348 GNKKDERSKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKA 407
DER +L EL + AF IG ++VLFE G G T + A
Sbjct: 364 AAVMDERLVRLQELIDSQQAAFNAAVIGTTVEVLFERAAR-NPGQIVGRTAYLQPAHVMA 422
Query: 408 DCNVSGKILNVKINSFEGEVAEGEI 432
++ G++L V+I+S E GE+
Sbjct: 423 APDIIGQVLPVRIDSLERYSLIGEL 447
>MIAB_PARDP RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 445
Score = 191 bits (484), Expect = 1e-50
Identities = 134/439 (30%), Positives = 213/439 (48%), Gaps = 20/439 (4%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIG 61
K+ T GC++N Y+S+ M E EGY + + AD+ ++NTC + +K +G
Sbjct: 14 KLFIKTYGCQMNVYDSQRMAEAMGAEGYVLTENQSDADMVLLNTCHIREKAAEKLYSDLG 73
Query: 62 RAR---RQNPEAIIAVVGCYAQIAPTEVSG-IEGVDVVLGSRNKGEIVYYVNKARDEGKQ 117
R + + P+ I V GC AQ E+ + VD+V+G + Y+ A +
Sbjct: 74 RLKPLKAERPDLKIGVAGCVAQAEGEEIQRRMPIVDLVVGPQ-----AYHRLPAMARAGR 128
Query: 118 QVKVGAVLKNKVFEDL-KIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
V ++K FE L K FL +Q+GC++FCA+C++PYTRG+ S+ ++
Sbjct: 129 GVDTEFPAEDK-FEHLPKXXXXXXXXXXFLTVQEGCDKFCAFCVVPYTRGAEVSRPVSRI 187
Query: 177 LDEIRSLAEHGFKEIILSGIHTASYGVDLDE--KVTLVDLLEEIEKIDGIERVRIGSIDP 234
L E R L G +EI L G + + + E + L+ I +IDG++R+R + P
Sbjct: 188 LAEARDLVARGVREITLLGQNVNGWHGEGPEGSEWGFGRLIRAIAEIDGLDRIRYTTSHP 247
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
+D++ KL P+ HL +QSG D LK MNRR+T +Y ++ +R+ D+
Sbjct: 248 NDMADDLIAAHRDEPKLMPYLHLPVQSGSDRILKAMNRRHTVDQYLRLIERIREARPDIM 307
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
+T+D IVGFPG ++ + FKYSPR GT A +++G D R
Sbjct: 308 LTSDFIVGFPGETDQDHQGTLELVRAVNFGTAFSFKYSPRPGTPAYE-RPEIEGAVADAR 366
Query: 355 SKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSGK 414
++L L +KA E +G + VLFE+ G G + V +A G
Sbjct: 367 LQELQALLTSQQKAAQEGMVGRELGVLFEKPGR-NPGQMVGKSDYLHAVFVEAPAAKVGD 425
Query: 415 ILNVKI-----NSFEGEVA 428
++ V+I NS G +A
Sbjct: 426 LVRVRITHSAPNSLAGVLA 444
>MIAB_MYCUA RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 532
Score = 191 bits (484), Expect = 1e-50
Identities = 121/385 (31%), Positives = 183/385 (47%), Gaps = 12/385 (3%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKK---SRQIIGRA 63
T GC++N ++SE M GY ADV V NTC+V D K + +
Sbjct: 30 TYGCQMNVHDSERMAGLLEAAGYRRAAEGTDADVVVFNTCAVRENADNKLYGNLSHLAPR 89
Query: 64 RRQNPEAIIAVVGCYAQI-APTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVKVG 122
+R +P+ IAV GC AQ + VDVV G+ N G + +++AR QV++
Sbjct: 90 KRTSPDMQIAVGGCLAQKDRDALLRKAPWVDVVFGTHNIGSLPALLDRARHNRVAQVEIA 149
Query: 123 AVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDEIRS 182
L+ F + A++ I GCN C +C++P RG + P +L E++S
Sbjct: 150 EALQQ--FPSSLPSARESAYAAWVSISVGCNNTCTFCIVPSLRGKEIDRSPADILAEVQS 207
Query: 183 LAEHGFKEIILSGIHTASYGVDLDEKV------TLVDLLEEIEKIDGIERVRIGSIDPTF 236
L + G EI L G + +YGV + +LL IDG+ERVR S P
Sbjct: 208 LVDTGVVEITLLGQNVNAYGVSFADPALPRNRGAFAELLRACGDIDGLERVRFTSPHPAE 267
Query: 237 FTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSIT 296
FT+DV+ + +CP H+ LQSG D L+ M R Y A+ Y I+ +R + +IT
Sbjct: 268 FTDDVIEAMAQTPNVCPALHMPLQSGSDRVLRAMRRSYRAERYLGIIERVRAAMPHAAIT 327
Query: 297 TDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSK 356
TD+IVGFPG +++ S F+YS R GT A ++ Q+ ER +
Sbjct: 328 TDLIVGFPGETEQDFAATLDVVRQARFSAAFTFQYSKRPGTPAAELDGQLPKAVVQERYE 387
Query: 357 KLIELNRINEKAFAEKYIGEVIDVL 381
+L+EL +G+ +++L
Sbjct: 388 RLVELQEQISLEGNRAIVGQRVELL 412
>MIAB_MYCS2 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 512
Score = 191 bits (484), Expect = 1e-50
Identities = 122/385 (31%), Positives = 188/385 (48%), Gaps = 12/385 (3%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKK---SRQIIGRA 63
T GC++N ++SE ++ GY+ AD+ V NTC+V D K + +
Sbjct: 29 TYGCQMNVHDSERLSGLLESAGYQRAAEGTDADIVVFNTCAVRENADNKLYGNLSHLAPR 88
Query: 64 RRQNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVKVG 122
++ +P IAV GC AQ V VDVV G+ N G + + +AR + QV++
Sbjct: 89 KQADPNMQIAVGGCLAQKDRDAVLRRAPWVDVVFGTHNIGSLPTLLERARHNREAQVEIV 148
Query: 123 AVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDEIRS 182
L+ F + A++ I GCN C +C++P RG + P +L E++S
Sbjct: 149 EALQE--FPSALPASRESAYAAWVSISVGCNNTCTFCIVPSLRGKEVDRRPGDILAEVQS 206
Query: 183 LAEHGFKEIILSGIHTASYGVDLDE------KVTLVDLLEEIEKIDGIERVRIGSIDPTF 236
L + G EI L G + +YGV + + LL IDG+ERVR S P
Sbjct: 207 LVDQGVLEITLLGQNVNAYGVSFADPELPRDRGAFAKLLRACGGIDGLERVRFTSPHPAE 266
Query: 237 FTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSIT 296
FT+DV+ + A +CP H+ LQSG D LK M R Y A+ + I++ +R I +IT
Sbjct: 267 FTDDVIEAMAATPNVCPTLHMPLQSGSDRILKAMRRSYRAERFLGIIDKVRAAIPHAAIT 326
Query: 297 TDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSK 356
TD+IVGFPG +++ + F+YS R GT A +M +Q+ + ER +
Sbjct: 327 TDLIVGFPGETEEDFQATLDVVEQARFAGAFTFQYSKRPGTPAADMPDQLPKSVVTERYQ 386
Query: 357 KLIELNRINEKAFAEKYIGEVIDVL 381
+LIEL + +G +++L
Sbjct: 387 RLIELQERISLEQNREQVGRAVELL 411
>MIAB_GEOLS RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 438
Score = 191 bits (484), Expect = 1e-50
Identities = 124/435 (28%), Positives = 213/435 (48%), Gaps = 11/435 (2%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIG 61
K+ T+GC++N +SE + GY A + + NTC+V ++ Q I
Sbjct: 5 KLYIDTVGCQMNVNDSERIVTMLQPLGYTQTSRRHEAALILFNTCTVRAGAEECLLQNIA 64
Query: 62 RAR---RQNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGKQ 117
+ R+ P +I V GC AQ E+ VD+V G+ N +V + K + G++
Sbjct: 65 NLKNLKRKKPGTLIGVAGCVAQQMGAELLQKFPWVDLVFGTHNL-HLVPEMVKDAEAGRR 123
Query: 118 QVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVL 177
+ + + ++ + E + AF+ + GC+ FC+YC++PY RG S+ ++L
Sbjct: 124 RAETDFLDSSERLDLFPPIEGRKRVSAFVTVMQGCDNFCSYCIVPYVRGREISRRFAEIL 183
Query: 178 DEIRSLAEHGFKEIILSGIHTASYGVDLDEKVTLVDLLEEIEKIDGIERVRIGSIDPTFF 237
E++ LA G +E++L G + SYG+ +E+ + +L+ + + GI+RVR + P
Sbjct: 184 QEVQDLAAQGLREVVLLGQNVNSYGLKGEEQPSFAELVRAVAAVSGIDRVRFVTSHPKDM 243
Query: 238 TEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITT 297
++D++ L KLC HL Q+G + LK MNR Y+ + Y + + LR ++ IT
Sbjct: 244 SDDLIACFADLAKLCGSLHLPAQAGNNRILKAMNRGYSREHYLETIYKLRQARPEIKITG 303
Query: 298 DVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSKK 357
D+IVGFPG ++ + F YSPR GT+A + +++ K R +
Sbjct: 304 DMIVGFPGETEAEFEETLSLMEEVRYFDLFSFVYSPRPGTKAAELSDELAKEVKLARLDR 363
Query: 358 LIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSGKILN 417
L +L ++ + E Y+G VL E + G +G + VN + GK+++
Sbjct: 364 LQKLQAVHSRIHNETYVGSTQQVLV-EGLAKRHGQVSGRCDSGRIVNLAGSPALIGKLVD 422
Query: 418 VKI-----NSFEGEV 427
VKI NS GE+
Sbjct: 423 VKILEGYANSLLGEL 437
>MIAB_GEOBB RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 441
Score = 191 bits (484), Expect = 1e-50
Identities = 129/438 (29%), Positives = 211/438 (48%), Gaps = 10/438 (2%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIG 61
K+ T GC++N +SE + GY+ AD+ ++NTCS+ +++ +G
Sbjct: 6 KLYLETFGCQMNVSDSEKIVTLMKGMGYQQTQDPVDADLVLLNTCSIRATAEQRVYGHLG 65
Query: 62 R---ARRQNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGKQ 117
+ ++ P II V GC AQ ++ V++V G+ N ++ + A +EGK+
Sbjct: 66 KFKSIKKTKPGLIIGVGGCVAQQEGEKLLKKAPFVNLVFGTHNL-HLLQGMVAAAEEGKR 124
Query: 118 QVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVL 177
+ + K F+ E F+ + GC+ FCAYC++P+ RG S+ KV+
Sbjct: 125 SSQTDFLDDEKRFDLFPHSEAEGGVTRFVTVMQGCDNFCAYCIVPHVRGREISRSAAKVV 184
Query: 178 DEIRSLAEHGFKEIILSGIHTASYGVDLDEKVTLVDLLEEIEKIDGIERVRIGSIDPTFF 237
+E+R+LA+ G E+ L G + SY + DLL + ++DGIER+R + P
Sbjct: 185 EEVRALADSGVTEVTLLGQNVNSYCSKQPGEPDFPDLLRLVAQVDGIERIRFTTSHPKDM 244
Query: 238 TEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITT 297
+ ++ L KL PH HL QSG D L+RMNR YTA++Y V L++ + T
Sbjct: 245 SPRLIECFADLPKLAPHIHLPAQSGSDRVLERMNRGYTAQQYLAKVAALKEACPAIQFTG 304
Query: 298 DVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSKK 357
D+IVGFPG ++++ + F YS R GT+A + +K R ++
Sbjct: 305 DMIVGFPGEDEAAFQDTMALMEQVQYADLFSFIYSARPGTKAAEYADDATRAEKQGRLER 364
Query: 358 LIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVY--TGYTRNYIKVNAKADCNVSGKI 415
L + A G V VL E G ++ TG R + D +++G++
Sbjct: 365 LQAAQKKTTLARNRSLEGTVQKVLVEGLSSTGDSLFGRTGGNRGTVMA---GDPSLAGRV 421
Query: 416 LNVKINSFEGEVAEGEIV 433
L+VKI + +GEIV
Sbjct: 422 LDVKIVEGLQTLLKGEIV 439
>MIAB_ACAM1 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 454
Score = 191 bits (484), Expect = 1e-50
Identities = 128/443 (28%), Positives = 217/443 (48%), Gaps = 41/443 (9%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIGR-ARR 65
T GC++N +SE M GY+ + + A++ + NTC++ + + K +GR A+R
Sbjct: 12 TYGCQMNKADSERMAGVLENMGYQWSENPDDANLILCNTCTIRDNAEHKVYSYLGRQAKR 71
Query: 66 QN--PEAIIAVVGCYAQI-APTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVKVG 122
++ P+ + V GC AQ + + +D+V+G + + N+ +D +Q
Sbjct: 72 KHAQPDLTLVVAGCVAQQEGDALLRRVPELDLVMGPQ-------HANRLQDLLEQVASGQ 124
Query: 123 AVLKNK---VFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDE 179
VL + + ED+ ++ A++ + GCN C YC++P RG S+ P+ + E
Sbjct: 125 QVLATEPIHIVEDITKPRRDSAVTAWVNVIYGCNERCTYCVVPNVRGVEQSRTPEAIRAE 184
Query: 180 IRSLAEHGFKEIILSGIHTASYGVDLD-------EKVTLVDLLEEIEKIDGIERVRIGSI 232
+ L E GFKE+ L G + +YG DL + TL DLL + + GIER+R +
Sbjct: 185 MVQLGEQGFKEVTLLGQNIDAYGRDLPGTTSEGRHQHTLTDLLYFVHDVPGIERIRFATS 244
Query: 233 DPTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIED 292
P +FTE +++ L K+C HFH+ QSG + LK M+R YT ++Y I++ +R + D
Sbjct: 245 HPRYFTERLIQACYELPKVCEHFHIPFQSGDNDVLKAMSRGYTHEKYRRIIDNIRAIMPD 304
Query: 293 VSITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKD 352
SI+ D IVGFPG ++ I + YSPR GT A +NQ+ K
Sbjct: 305 ASISADAIVGFPGETEEQFMNTMQLVEDIEFDLLNTAAYSPRPGTPAALWDNQLSEEVKA 364
Query: 353 ERSKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVS 412
+R ++L L + + +++Y + +VL E++ N K V
Sbjct: 365 DRLQRLNRLVGVCAELRSQRYANRIEEVLVEDQ------------------NPKDPTQVM 406
Query: 413 GKILNVKINSFEGEVAE--GEIV 433
G+ ++ F G + + GEI+
Sbjct: 407 GRTRGNRLTFFPGNIEQLRGEII 429
>MIAB2_MYCMM RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB 2;
EC=2.-.-.-;AltName: Full=tRNA-i(6)A37
methylthiotransferase 2;
Length = 532
Score = 191 bits (484), Expect = 1e-50
Identities = 121/385 (31%), Positives = 183/385 (47%), Gaps = 12/385 (3%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKK---SRQIIGRA 63
T GC++N ++SE M GY ADV V NTC+V D K + +
Sbjct: 30 TYGCQMNVHDSERMAGLLEAAGYRRAAEGTDADVVVFNTCAVRENADNKLYGNLSHLAPR 89
Query: 64 RRQNPEAIIAVVGCYAQI-APTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVKVG 122
+R +P+ IAV GC AQ + VDVV G+ N G + +++AR QV++
Sbjct: 90 KRTSPDMQIAVGGCLAQKDRDALLRKAPWVDVVFGTHNIGSLPALLDRARHNRVAQVEIA 149
Query: 123 AVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDEIRS 182
L+ F + A++ I GCN C +C++P RG + P +L E++S
Sbjct: 150 EALQQ--FPSSLPSARESAYAAWVSISVGCNNTCTFCIVPSLRGKEIDRSPADILAEVQS 207
Query: 183 LAEHGFKEIILSGIHTASYGVDLDEKV------TLVDLLEEIEKIDGIERVRIGSIDPTF 236
L + G EI L G + +YGV + +LL IDG+ERVR S P
Sbjct: 208 LVDTGVVEITLLGQNVNAYGVSFADPALPRNRGAFAELLRACGDIDGLERVRFTSPHPAE 267
Query: 237 FTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSIT 296
FT+DV+ + +CP H+ LQSG D L+ M R Y A+ Y I+ +R + +IT
Sbjct: 268 FTDDVIEAMAQTPNVCPALHMPLQSGSDRVLRAMRRSYRAERYLGIIERVRAAMPHAAIT 327
Query: 297 TDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSK 356
TD+IVGFPG +++ S F+YS R GT A ++ Q+ ER +
Sbjct: 328 TDLIVGFPGETEQDFAATLDVVRQARFSAAFTFQYSKRPGTPAAELDGQLPKAVVQERYE 387
Query: 357 KLIELNRINEKAFAEKYIGEVIDVL 381
+L+EL +G+ +++L
Sbjct: 388 RLVELQEQISLEGNRAIVGQRVELL 412
>MIAB_YERE8 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 474
Score = 190 bits (483), Expect = 2e-50
Identities = 130/428 (30%), Positives = 208/428 (48%), Gaps = 13/428 (3%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVR-EGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S M + GY++ + E AD+ ++NTCS+ +K ++
Sbjct: 4 KLHIKTWGCQMNEYDSSKMADLLASTHGYQLTEIPEEADLLLLNTCSIREKAQEKVFSLL 63
Query: 61 GR---ARRQNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
G+ + +NPE II V GC A + VDV+ G + + +N +
Sbjct: 64 GQWKLLKEKNPELIIGVGGCVASQEGEHLRQRAPCVDVIFGPQTLHRLPEMINHVQGTHS 123
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
V + K F+ L T AF+ I +GCN++C +C++PYTRG S+ +
Sbjct: 124 PVVDISFPEIEK-FDRLPEPRAEGPT-AFVSIMEGCNKYCTFCVVPYTRGEEVSRPSDDI 181
Query: 177 LDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKV-TLVDLLEEIEKIDGIERVRIGSIDP 234
L EI LA G +E+ L G + +Y G D + + +LL + IDGI+RVR + P
Sbjct: 182 LFEIAQLAAQGVREVNLLGQNVNAYRGATYDGDICSFAELLRLVAAIDGIDRVRFTTSHP 241
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
FT+D++ +L HL +QSG D L M R +TA EY+ I+ LR D+
Sbjct: 242 IEFTDDIIDVYRDTPELVSFLHLPVQSGSDRILTMMKRAHTALEYKAIIRKLRQARPDIQ 301
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
I++D I+GFPG + + ++ F YSPR GT A ++ + V +K +R
Sbjct: 302 ISSDFIIGFPGETQQDFEQTMKLVADVRFDTSYSFIYSPRPGTPAADLPDDVSEEEKKQR 361
Query: 355 SKKLIELNRINEKA--FAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVS 412
L + RI ++A + + +G V +L E G T N VN + +
Sbjct: 362 LHILQQ--RITQQAMEISREMVGTVQRILVEGTSRKNVMELAGRTENNRVVNFEGTPEMI 419
Query: 413 GKILNVKI 420
GK ++V+I
Sbjct: 420 GKFVDVEI 427
>MIAB_SHESH RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 474
Score = 190 bits (483), Expect = 2e-50
Identities = 135/444 (30%), Positives = 221/444 (49%), Gaps = 18/444 (4%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVR-EGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S M + EGY + + ADV ++NTCS+ +K +
Sbjct: 4 KLHIKTWGCQMNEYDSSKMADLMDEYEGYTLTEEASEADVLLLNTCSIREKAQEKVFHQL 63
Query: 61 GRARR---QNPEAIIAVVGCYA-QIAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR ++ +NP+ II V GC A Q + VD++ G + + +++ RD GK
Sbjct: 64 GRWKKLKEKNPDLIIGVGGCVASQEGKVIKERAQCVDLIFGPQTLHRLPEMIDQIRDGGK 123
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
+ + K F+ L E + AF+ I +GC+++C++C++PYTRG S+ +
Sbjct: 124 AVIDISFPEIEK-FDKLP-EPRADGPSAFVSIMEGCSKYCSFCVVPYTRGEEVSRPLDDI 181
Query: 177 LDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKV-TLVDLLEEIEKIDGIERVRIGSIDP 234
+ EI LA+ G +E+ L G + +Y G D+++ T +LL + IDGI+R+R + P
Sbjct: 182 ILEIAQLADQGVREVNLLGQNVNAYRGATHDDEICTFAELLRYVAAIDGIDRLRFTTSHP 241
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
FT+D++ +L HL +QSG D L +M R + A EY+ I+ LR D+
Sbjct: 242 IEFTQDIIDVYEDTPELVSFLHLPVQSGSDRILTQMKRGHMAIEYKSIIRRLRKARPDIQ 301
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
I++D I+GFPG ++ + + F YS R GT A ++ + V +K ER
Sbjct: 302 ISSDFIIGFPGESKQDFADTMKLIEDVQFDHSFSFIYSARPGTPAADLPDDVTLAEKKER 361
Query: 355 SKKLIELNRINEKA--FAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVS 412
I +RI ++A ++ + +G V +L E G T VN +AD
Sbjct: 362 --LAILQDRITQQAMRYSRQMLGTVQRILVEGPSVKNPMELRGRTETSRVVNFEADPKHI 419
Query: 413 GKILNVKI-----NSFEGEVAEGE 431
G ++V+I NS G GE
Sbjct: 420 GSFVDVEIVDVYTNSLRGTFVRGE 443
>MIAB_SHEAM RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 474
Score = 190 bits (483), Expect = 2e-50
Identities = 131/442 (29%), Positives = 215/442 (48%), Gaps = 14/442 (3%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVR-EGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S M + +GY + + E AD+ ++NTCS+ +K +
Sbjct: 4 KLHIKTWGCQMNEYDSSKMADLLGEYQGYTLTEEAEEADILLLNTCSIREKAQEKVFHQL 63
Query: 61 GR---ARRQNPEAIIAVVGCYAQIAPTEVSG-IEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR + +NP+ II V GC A + + VD++ G + + +++ R K
Sbjct: 64 GRWKTLKDKNPDLIIGVGGCVASQEGKAIKDRAQCVDIIFGPQTLHRLPEMIDQVRSGEK 123
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
+ V K F+ L T AF+ I +GC+++C++C++PYTRG S+ +
Sbjct: 124 AVIDVSFPEIEK-FDRLPEPRAEGPT-AFVSIMEGCSKYCSFCVVPYTRGEEVSRPMDDI 181
Query: 177 LDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKV-TLVDLLEEIEKIDGIERVRIGSIDP 234
+ EI LAE G +E+ L G + +Y G D ++ T +LL + IDGI+R+R + P
Sbjct: 182 ILEIAQLAEQGVREVNLLGQNVNAYRGAKHDGEICTFAELLRHVAAIDGIDRLRFTTSHP 241
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
FT+D++ +L HL +QSG D L M R + A EY+ I+ LR D+
Sbjct: 242 IEFTQDIIDVYEDTPELVSFLHLPVQSGSDRILTAMKRGHMAIEYKSIIRRLRKARPDIQ 301
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
I++D IVGFPG ++ + + F YS R GT A ++ + VD +K +R
Sbjct: 302 ISSDFIVGFPGETAQDFQDTMKLIEDVNFDMSFSFIYSARPGTPAADLPDDVDMEEKKQR 361
Query: 355 SKKLIELNRINEKAFAEKYIGEVIDVLFE-----EEVELGSGVYTGYTRNYIKVNAKADC 409
L EL ++ + +G V +L E +EL T N+ ++
Sbjct: 362 LADLQELINQQAMRYSRQMMGTVQRILVEGPSVKNPMELRGRTETNRVVNFEGLHKHIGT 421
Query: 410 NVSGKILNVKINSFEGEVAEGE 431
V +I++V NS G+ GE
Sbjct: 422 FVDVEIVDVFPNSLRGKFIRGE 443
>MIAB_RHOS5 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 436
Score = 190 bits (483), Expect = 2e-50
Identities = 133/434 (30%), Positives = 214/434 (49%), Gaps = 16/434 (3%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIG 61
K+ T GC++N Y+SE M E +GY + + E AD+ ++NTC + +K +G
Sbjct: 6 KLFIKTYGCQMNVYDSERMAEALGAKGYVLTEVAEEADMVLLNTCHIREKAAEKVYSDLG 65
Query: 62 RARR---QNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGKQ 117
R R P+ I V GC AQ E+ + VD+V+G ++ + + R EG
Sbjct: 66 RLRPLKVARPDLKIGVAGCVAQAEGEEILRRMPLVDLVVGPQSYHRLPDMLE--RTEGGA 123
Query: 118 QVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVL 177
+V + FE L + AFL +Q+GC++FCA+C++PYTRG+ S+ +++
Sbjct: 124 RVIDTDFPEEDKFEHLPERKALRGPTAFLTVQEGCDKFCAFCVVPYTRGAEVSRPFARLM 183
Query: 178 DEIRSLAEHGFKEIILSGIHTASYGVDLDEKVTLVDLLEEIEKIDGIERVRIGSIDPTFF 237
E R+L E G +EI L G + ++ D L L+ E+ +IDG+ER+R + P
Sbjct: 184 AEARALVEKGVREITLLGQNVNAWSND---GRGLGGLIRELARIDGLERLRYTTSHPNDM 240
Query: 238 TEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITT 297
+D++ KL P+ HL +QSG D LK MNR++ A++Y +V +R D+ +T+
Sbjct: 241 ADDLIEAHGQEPKLMPYLHLPVQSGSDRILKAMNRKHAAEQYLRLVERIRAARPDILLTS 300
Query: 298 DVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSKK 357
D IVGFPG ++ + FKYS R GT A + ++ D R +
Sbjct: 301 DFIVGFPGETDADFEATLALIRAVGFGSAFSFKYSARPGTPAAE-KPELPAELCDARLQT 359
Query: 358 LIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSGKILN 417
L L ++A +G + VL+E+ L G G + V+ + G ++
Sbjct: 360 LQALLGEQQRAAQAAMVGRELGVLYEKAGRL-PGQMVGKSDYLHAVHVEDPGARPGDLVR 418
Query: 418 VKI-----NSFEGE 426
V+I NS GE
Sbjct: 419 VRITASGPNSLAGE 432
>MIAB_PROMP RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 464
Score = 190 bits (483), Expect = 2e-50
Identities = 125/442 (28%), Positives = 212/442 (47%), Gaps = 20/442 (4%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIGRA--- 63
T GC++N +SE M + GY AD+ + NTC++ + +K +G+
Sbjct: 25 TFGCQMNKADSERMAGTLEKMGYSRAIDELKADLVLYNTCTIRDSAQQKVYSFLGKQVKR 84
Query: 64 RRQNPEAIIAVVGCYAQI-APTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVKVG 122
+ P+ + V GC AQ + + + +D+++G ++ + + + D G Q V
Sbjct: 85 KHSTPKLKLVVAGCLAQQEGESLLRRVPELDLIMGPQHVNNLENLLERV-DLGNQVV--- 140
Query: 123 AVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDEIRS 182
A + + ED+ ++ ++ I GCN C+YC++P RG S+ P + EI S
Sbjct: 141 ATEETFISEDITNPRRDSSICGWVNIIYGCNERCSYCVVPSVRGKEQSRYPDAIKSEIES 200
Query: 183 LAEHGFKEIILSGIHTASYGVDLD-------EKVTLVDLLEEIEKIDGIERVRIGSIDPT 235
LA + FKE+ L G + +YG DL ++ +L DLL I ++GI+R+R + P
Sbjct: 201 LAHNNFKEVTLLGQNIDAYGRDLPGTTKEGRKENSLTDLLYFIHDVEGIKRIRFSTSHPK 260
Query: 236 FFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSI 295
+F++ ++ L K+C HFH+ QSG + L+ M R YT ++Y+ I+ +R + + SI
Sbjct: 261 YFSKRLINACYELDKVCEHFHIPFQSGNNEILRLMARGYTIEKYKRIIENIRQLMPNASI 320
Query: 296 TTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERS 355
T D IV FPG + I + YSPR T A +NQ+ K ER
Sbjct: 321 TADAIVAFPGETETQYRETLKLISDIGFDQVMTAAYSPRPNTPAAFWDNQIPEEVKKERL 380
Query: 356 KKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTR----NYIKVNAKADCNV 411
K++ EL + ++Y+ + VL E + G TR +++++ + N
Sbjct: 381 KEINELVETTSRKRNQRYLNNIESVLIEGLNPKNNAQMMGRTRTNRLTFVEISKNIEFNY 440
Query: 412 S-GKILNVKINSFEGEVAEGEI 432
S G +NVKI G+I
Sbjct: 441 SYGDEINVKITEARSFSLSGQI 462
>MIAB_PHOLL RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 476
Score = 190 bits (483), Expect = 2e-50
Identities = 133/444 (29%), Positives = 214/444 (48%), Gaps = 18/444 (4%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVR-EGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S M + GY++ D E AD+ ++NTCS+ +K +
Sbjct: 6 KLYIKTWGCQMNEYDSSKMADLLESTHGYQLTDVAEEADILLLNTCSIREKAQEKVFHQL 65
Query: 61 GRARR---QNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR + NP+ II V GC A + + VD++ G + + +N+ +
Sbjct: 66 GRWKNLKDTNPDLIIGVGGCVASQEGDFIRQRAQCVDIIFGPQTLHRLPEMINQVKGTRS 125
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
V + K D E AF+ I +GCN++C +C++PYTRG S+ V
Sbjct: 126 PVVDISFPEIEKF--DRLPEPRAEGPSAFVSIMEGCNKYCTFCVVPYTRGEEVSRPCDDV 183
Query: 177 LDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKV-TLVDLLEEIEKIDGIERVRIGSIDP 234
L EI LA G +E+ L G + +Y G D + + +L+ + IDGI+R+R + P
Sbjct: 184 LFEIAQLAAQGVREVNLLGQNVNAYRGATYDGDICSFAELIRLVAAIDGIDRIRFTTSHP 243
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
FT+D++ +L HL +QSG D L M R +TA EY+ I+ LR D+
Sbjct: 244 IEFTDDIIAVYEDTPELVSFLHLPVQSGSDRILTLMKRAHTALEYKSIIRKLRKARPDIL 303
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
I++D I+GFPG + + ++ F YS R GT A ++ + V +K +R
Sbjct: 304 ISSDFIIGFPGETQDDFEKTMKLIADVNFDMSYSFIYSARPGTPAADLPDDVSEEEKKQR 363
Query: 355 SKKLIELNRINEKA--FAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVS 412
L + RIN++A ++ +G V +L E +G T N VN + ++
Sbjct: 364 LYLLQQ--RINQQAMSYSRAMLGSVQRILVEGTSRKNVMELSGRTENNRVVNFEGQPDMI 421
Query: 413 GKILNVKI-----NSFEGEVAEGE 431
GK ++V+I NS G+V E
Sbjct: 422 GKFVDVEIVDVYANSLRGKVIRTE 445
>MIAB_NITEC RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 443
Score = 190 bits (483), Expect = 2e-50
Identities = 131/438 (29%), Positives = 205/438 (46%), Gaps = 9/438 (2%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVRE-GYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S M + + E E+ + E AD+ + NTCSV +K +
Sbjct: 4 KLYIKTFGCQMNEYDSAKMADILLSEKNMELTEVPEEADLILFNTCSVREKAQEKVFHDL 63
Query: 61 GRARR---QNPEAIIAVVGCYAQIAPTE-VSGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR R P+ +I V GC A +E V VD+V G + + ++ R G+
Sbjct: 64 GRVRHLKNSKPDLLIGVGGCVASQEGSEIVRRAPFVDLVFGPQTLHRLPELIDARRRTGQ 123
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
QV + K F+ L T AF+ I +GC+++C++C++PYTRG S+ V
Sbjct: 124 SQVDITFPEIEK-FDRLPPARTTGAT-AFVSIMEGCSKYCSFCVVPYTRGEEVSRPLDDV 181
Query: 177 LDEIRSLAEHGFKEIILSGIHTASY--GVDLDEKVTLVDLLEEIEKIDGIERVRIGSIDP 234
L E+ L G KE+ L G + +Y + + LL+ I +I G+ R+R + P
Sbjct: 182 LTEVAGLVIQGVKEVTLLGQNVNAYYDKTSGEGDIDFATLLDYIHEIPGLVRIRYTTSHP 241
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
FT ++ L KL H HL +QSG D L M R YT EY+ I+ LR ++S
Sbjct: 242 REFTARLIETYQRLPKLVGHVHLPIQSGSDRILAAMKRGYTIIEYKSIIRKLRTIRPNIS 301
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
I++D IVGFPG + + ++ F YSPR GT A ++ + K R
Sbjct: 302 ISSDFIVGFPGETDTDFEETMKLIDDVKFDESFSFIYSPRPGTPASDLPDDTPYRIKLAR 361
Query: 355 SKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSGK 414
+L E + N + ++ + + VL E + + G T N VN + G
Sbjct: 362 LHQLQEKIQRNAQMISQSMVDTIQRVLVEGPSKKDPNEFCGRTDNNRVVNFAGHAGLVGS 421
Query: 415 ILNVKINSFEGEVAEGEI 432
+++KI + GEI
Sbjct: 422 FVDIKITAVSSHTLRGEI 439
>MIAB_COLP3 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 480
Score = 190 bits (483), Expect = 2e-50
Identities = 129/439 (29%), Positives = 208/439 (47%), Gaps = 9/439 (2%)
Query: 2 KVAFATLGCRVNTYESEAMTEKF-VREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S+ M E G+ +V+ E ADV ++NTCS+ +K +
Sbjct: 4 KLYIKTWGCQMNEYDSQKMAELLDSTHGFSLVEEAEQADVILLNTCSIREKAQEKVFHQL 63
Query: 61 GRARR---QNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR + + P+ +I V GC A + VD++ G + + +N+ +
Sbjct: 64 GRWKNLKDKKPDLLIGVGGCVASQEGDSIRKRAPFVDMIFGPQTLHRLPEMLNQLQHSKS 123
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
+ V K D E + AF+ I +GC+++C +C++PYTRG S+ V
Sbjct: 124 PIIDVSFPEIEKF--DRLPEPKADGASAFVSIMEGCSKYCTFCVVPYTRGEEVSRPLDDV 181
Query: 177 LDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKVT-LVDLLEEIEKIDGIERVRIGSIDP 234
L EI LAE G +E+ L G + +Y G D + DL+ + IDGI+R+R + P
Sbjct: 182 LYEIAQLAEQGVREVNLLGQNVNAYRGETHDGSICRFADLVRLVATIDGIDRIRYTTSHP 241
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
FT+D++ + +L H HL +QSGCD L +M R +TA EY+ + L+ ++S
Sbjct: 242 VEFTDDIIEAYTDVPELVNHLHLPVQSGCDRILTQMKRGHTALEYKSQIRKLKKVRPELS 301
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
+++D I+GFPG +K + + F YS R GT A ++ + + K +R
Sbjct: 302 MSSDFIIGFPGETDEDFTATMDLIKAVDFDLSFSFIYSARPGTPAADLPDDISDQTKKDR 361
Query: 355 SKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSGK 414
K L E A + + VL E G T N VN A +V G+
Sbjct: 362 LKLLQEQITHQALRIARQMLNTEQRVLVEGPSRKNPMELRGKTENNRTVNFVAPHSVIGQ 421
Query: 415 ILNVKINSFEGEVAEGEIV 433
+++KI GE+V
Sbjct: 422 FVDIKITDVVANSLRGELV 440
>MIAB_PSYWF RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 490
Score = 190 bits (482), Expect = 2e-50
Identities = 138/456 (30%), Positives = 215/456 (47%), Gaps = 31/456 (6%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVREGYEVVDFN-EMADVYVVNTCSVTNMGDKKSRQII 60
KV AT GC++N Y+SE M VV N E ADV ++NTCS+ +K +
Sbjct: 38 KVYIATQGCQMNVYDSEKMGNVLGDSHDMVVTDNIEEADVLLMNTCSIREKAQEKVFSEL 97
Query: 61 GRARR---QNPEAIIAVVGCYAQIAPTEVSGIEG----VDVVLGSRNKGEIVYYVNKARD 113
GR R+ + P+ +I V GC +A E I+ VD+V G + + +K+
Sbjct: 98 GRWRKLKEEKPDLVIGVGGC---VASQEGDNIQKRAPYVDMVFGPQTLHRLPELYDKSTT 154
Query: 114 E---------GKQQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYT 164
+ G V ++ K + K+E Y RAF+ I +GC+++C++C++PYT
Sbjct: 155 QRNVKPKDRIGTVDVSFPSIEKFDFLPEPKVEGY----RAFVSIMEGCSKYCSFCVVPYT 210
Query: 165 RGSVCSKDPKKVLDEIRSLAEHGFKEIILSGIHTASYGVDLDEK--VTLVDLLEEIEKID 222
RG S+ VL EI SLAE G +EI L G + Y + D+ +LL + +D
Sbjct: 211 RGEELSRPLDDVLAEIDSLAEQGVREITLLGQNVNGYRGEKDDGSICRFAELLHYVAHVD 270
Query: 223 GIERVRIGSIDPTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDI 282
GIER+R + P FT+D++ L +L H HL +QSG + L M R +T Y +
Sbjct: 271 GIERIRYTTSHPLEFTDDIIEAYAKLPQLVSHLHLPVQSGSNKILAAMKRNHTIDVYINQ 330
Query: 283 VNLLRDKIEDVSITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENM 342
+N L D+ +++D I+GFPG K + ++ F YS R GT A +
Sbjct: 331 INKLMAVRPDMHLSSDFIIGFPGETEEDFLDTLNLAKALDFDHSYSFIYSKRPGTPASEL 390
Query: 343 ENQVDGNKKDERSKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRN--- 399
+ V K ER ++ R + E+ +G+ + V+ EE + G N
Sbjct: 391 PDDVSLATKKERLAIFQKVIRDSTLKKTEEMVGKTLRVMVEEIADRYPDQLLGTADNTRS 450
Query: 400 -YIKVNAKADCNVSGKILNVKINSF-EGEVAEGEIV 433
K + ++ GK + VKI F + GE+V
Sbjct: 451 VLFKATEQQKTDLMGKFVTVKITDFVSPHMVRGELV 486
>MIAB_PARD8 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 457
Score = 190 bits (482), Expect = 2e-50
Identities = 129/439 (29%), Positives = 219/439 (49%), Gaps = 10/439 (2%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKK---SRQ 58
K+ T GC++N +SE + +GY + + E AD VNTCSV + ++K Q
Sbjct: 20 KLFIETYGCQMNVADSEVVASIMKMDGYSMTENIEEADAIFVNTCSVRDNAEQKIYGRLQ 79
Query: 59 IIGRARRQNPEAIIAVVGCYAQIAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQ 118
+R+ I+ V+GC A+ ++ + D+V+G + ++ V A + G++
Sbjct: 80 YFQSLKRKKKSLIVGVLGCMAERVKEDLIHVHHADLVVGPDSYLDLPNLVG-AVEHGEKA 138
Query: 119 VKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLD 178
+ V + + + ++ F+ I GCN FC YC++PYTRG S+D + +L+
Sbjct: 139 INVELSTQETYKDVIPLKLPGVHISGFVSIMRGCNNFCTYCIVPYTRGRERSRDIESILN 198
Query: 179 EIRSLAEHGFKEIILSGIHTASYGVDLD-EKVTLVDLLEEIEKIDGIERVRIGSIDPTFF 237
EIR + E GFKE+ L G + SY + + E +T LL+ + K R+R + P
Sbjct: 199 EIRDMHEKGFKEVTLLGQNVNSYSFEKEGETITFPILLDRVAKEVPDMRIRFTTSHPKDM 258
Query: 238 TEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITT 297
++D +R I A +C HL QSG LK MNR+YT + Y D + +R + D +I+T
Sbjct: 259 SDDTLRVIAANDNICKFIHLPAQSGSSRILKVMNRKYTREWYLDRIAAIRRIVPDCAIST 318
Query: 298 DVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGT-RAENMENQVDGNKKDERSK 356
D+ GF ++ + +FKYS R GT A ++E+ V +K R +
Sbjct: 319 DLFCGFHSETEEDYQETLSLMREVGYDSAFLFKYSERPGTYAASHLEDNVPEEEKVRRLQ 378
Query: 357 KLIEL-NRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYT-RNYIKVNAKADCNVSGK 414
+I+L N+++E++ + IG+ +VL E + G T +N + + K + +V G+
Sbjct: 379 GMIDLQNKLSEESNL-RDIGKTFEVLIEGFSKRSREQLFGRTSQNKVVIFDKKNYHV-GQ 436
Query: 415 ILNVKINSFEGEVAEGEIV 433
+ VKI+ GE V
Sbjct: 437 FIKVKIHKASSATLFGEPV 455
>MIAB_SYNY3 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 451
Score = 189 bits (481), Expect = 3e-50
Identities = 125/438 (28%), Positives = 203/438 (46%), Gaps = 41/438 (9%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIGRARRQ 66
T GC++N +SE M G D AD+ + NTCS+ + ++K +GR ++
Sbjct: 12 TFGCQMNKADSERMAGILENLGMTYTDDPNQADLVLYNTCSIRDNAEQKVYSYLGRQAKR 71
Query: 67 N---PEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVKVG 122
PE + V GC AQ ++ + +D+V+G ++ + D+ +QV G
Sbjct: 72 KQVEPELTLVVAGCVAQQEGEQLLRRVPELDLVMGPQHANRL--------DQLLEQVWAG 123
Query: 123 ----AVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLD 178
A + ED+ + A++ I GCN C+YC++P RG S+ P+ +
Sbjct: 124 SQVVATESLHIMEDITKPRRESTVSAWVNIIYGCNERCSYCVVPNVRGVEQSRTPEAIYG 183
Query: 179 EIRSLAEHGFKEIILSGIHTASYGVDLDEKV-------TLVDLLEEIEKIDGIERVRIGS 231
E+ LA+ GFKE+ L G + +YG DL TL DLL + I+GI+R+R +
Sbjct: 184 EMEVLAQQGFKEVTLLGQNIDAYGRDLPGTTPSGRHLHTLTDLLYHVHDIEGIDRLRFAT 243
Query: 232 IDPTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIE 291
P +FTE +++ L K+C HFH+ QSG + LK M R YT ++Y I+ +R +
Sbjct: 244 SHPRYFTERLIQACQELPKVCEHFHIPFQSGDNDILKAMKRGYTREKYLQIIEKIRRYMP 303
Query: 292 DVSITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKK 351
D +I+ DVIVGFPG ++ + + YSPR GT A +NQ+ K
Sbjct: 304 DAAISADVIVGFPGETEAQFENTLNLIEEVGFDLLNTAAYSPRPGTPAAFWDNQLSEEVK 363
Query: 352 DERSKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNV 411
+R ++L L +++Y N K+ V
Sbjct: 364 GDRLQRLNHLVSTQAMERSQRY------------------XXXXXXXXXXXXNLKSPGQV 405
Query: 412 SGKILNVKINSFEGEVAE 429
G+ ++ F+GE++E
Sbjct: 406 MGRTRGNRLTFFQGEISE 423
>MIAB_LEPIN RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 449
Score = 189 bits (481), Expect = 3e-50
Identities = 125/442 (28%), Positives = 213/442 (48%), Gaps = 15/442 (3%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKK---SRQ 58
KV T GC++N Y+S ++ Y E +D+ +NTC++ K Q
Sbjct: 12 KVYIETYGCQMNEYDSGIVSSLMRDAEYSTSSDPENSDIIFLNTCAIRENAHAKIYNRLQ 71
Query: 59 IIGRARRQNPEAIIAVVGCYAQIAPTEVSGIE-GVDVVLGSRNKGEIVYYVNKARDEGKQ 117
+G +++NP +I V+GC AQ ++ E +D+V+G N + + + R +G+
Sbjct: 72 SLGYLKKRNPNLVIGVLGCMAQNLGDDLFHQELPLDLVVGPDNYRSLPELIQRIR-KGEN 130
Query: 118 QVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVL 177
+ + + K + +++++ N +AF+ I GCN FC +C++PYTRG S+DPK ++
Sbjct: 131 SISLTRLSKIETYDEIEPRVVNG-IQAFVTIMRGCNNFCTFCVVPYTRGRERSRDPKSIV 189
Query: 178 DEIRSLAEHGFKEIILSGIHTASYGVDLDEKVTLVDLLEEIEKIDGIERVRIGSIDPTFF 237
E++ L + G ++I L G + SY ++ L++ + IER+R S P F
Sbjct: 190 REVQDLVQKGIRQITLLGQNVNSYK---EQDTDFAGLIQMLLDETSIERIRFTSPHPKDF 246
Query: 238 TEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITT 297
+++ + + CP+ HL LQ+G L+ M R Y+ +E+ D+V +R+ + DV ITT
Sbjct: 247 PTHLLQLMSENPRFCPNIHLPLQAGNTRVLEEMKRSYSKEEFLDVVKEIRNIVPDVGITT 306
Query: 298 DVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAE-NMENQVDGNKKDERSK 356
D+IVGFP ++ + +FKYS R+GT A+ + + V K R
Sbjct: 307 DIIVGFPNETEEEFEDTLAVVREVQFDMAFMFKYSEREGTMAQRKLPDNVPEEVKSARLT 366
Query: 357 KLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYT--RNYIKVNAKADCNVSGK 414
KL++L IG V +L E G T + NVSG
Sbjct: 367 KLVDLQTSISHEQNRARIGRVYSILIENISRKSEKQLCGRTPCGRMTVFPLPQETNVSGM 426
Query: 415 I---LNVKINSFEGEVAEGEIV 433
I ++V+I S +G I+
Sbjct: 427 IGSTVSVQIESATSATLKGRIL 448
>MIAB_LEPIC RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 449
Score = 189 bits (481), Expect = 3e-50
Identities = 125/442 (28%), Positives = 213/442 (48%), Gaps = 15/442 (3%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKK---SRQ 58
KV T GC++N Y+S ++ Y E +D+ +NTC++ K Q
Sbjct: 12 KVYIETYGCQMNEYDSGIVSSLMRDAEYSTSSDPENSDIIFLNTCAIRENAHAKIYNRLQ 71
Query: 59 IIGRARRQNPEAIIAVVGCYAQIAPTEVSGIE-GVDVVLGSRNKGEIVYYVNKARDEGKQ 117
+G +++NP +I V+GC AQ ++ E +D+V+G N + + + R +G+
Sbjct: 72 SLGYLKKRNPNLVIGVLGCMAQNLGDDLFHQELPLDLVVGPDNYRSLPELIQRIR-KGEN 130
Query: 118 QVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVL 177
+ + + K + +++++ N +AF+ I GCN FC +C++PYTRG S+DPK ++
Sbjct: 131 SISLTRLSKIETYDEIEPRVVNG-IQAFVTIMRGCNNFCTFCVVPYTRGRERSRDPKSIV 189
Query: 178 DEIRSLAEHGFKEIILSGIHTASYGVDLDEKVTLVDLLEEIEKIDGIERVRIGSIDPTFF 237
E++ L + G ++I L G + SY ++ L++ + IER+R S P F
Sbjct: 190 REVQDLVQKGIRQITLLGQNVNSYK---EQDTDFAGLIQMLLDETSIERIRFTSPHPKDF 246
Query: 238 TEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITT 297
+++ + + CP+ HL LQ+G L+ M R Y+ +E+ D+V +R+ + DV ITT
Sbjct: 247 PTHLLQLMSENPRFCPNIHLPLQAGNTRVLEEMKRSYSKEEFLDVVKEIRNIVPDVGITT 306
Query: 298 DVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAE-NMENQVDGNKKDERSK 356
D+IVGFP ++ + +FKYS R+GT A+ + + V K R
Sbjct: 307 DIIVGFPNETEEEFEDTLAVVREVQFDMAFMFKYSEREGTMAQRKLPDNVPEEVKSARLT 366
Query: 357 KLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYT--RNYIKVNAKADCNVSGK 414
KL++L IG V +L E G T + NVSG
Sbjct: 367 KLVDLQTSISHEQNRARIGRVYSILIENISRKSEKQLCGRTPCGRMTVFPLPQETNVSGM 426
Query: 415 I---LNVKINSFEGEVAEGEIV 433
I ++V+I S +G I+
Sbjct: 427 IGSTVSVQIESATSATLKGRIL 448
>MIAB_GRAFK RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 481
Score = 189 bits (481), Expect = 3e-50
Identities = 126/452 (27%), Positives = 229/452 (50%), Gaps = 25/452 (5%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIG 61
K+ + GC +N +SE + +EG+ E AD+ +VNTCS+ ++ R+ +
Sbjct: 25 KLFIESYGCAMNFSDSEIVASILSKEGFNTTQNLEEADLVLVNTCSIREKAEQTVRKRLE 84
Query: 62 R---ARRQNPEAIIAVVGCYAQIAPTEVSGIEG-VDVVLGSRNKGEIVYYVNKARDEGKQ 117
+ +R NP + V+GC A+ + E VD+V+G ++ +N+ +EG+
Sbjct: 85 KYNAVKRINPGMKVGVLGCMAERLKDKFLEEEKIVDLVVGPDAYKDLPNLINEV-EEGRD 143
Query: 118 QVKVGAVLKNKVFEDLK-IEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
+ V + K + + D+ + +N AF+ I GC+ C +C++P+TRG S+DP+ +
Sbjct: 144 AINV-ILSKEETYGDISPVRLQSNGVSAFVSITRGCDNMCTFCVVPFTRGRERSRDPQSI 202
Query: 177 LDEIRSLAEHGFKEIILSGIHTASY-----GVDLD-------EKVTLVD---LLEEIEKI 221
++E+ LA G+KEI L G + SY G+ D +K T + LL+ + +
Sbjct: 203 VEEVNDLAAKGYKEITLLGQNVDSYLWYGGGLKKDFKNATEIQKATATNFAALLKLVAEA 262
Query: 222 DGIERVRIGSIDPTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYED 281
R+R + +P T DV+ + A + +C + HL +QSG D LK+MNR +T +EY
Sbjct: 263 QPKMRIRFSTSNPQDMTLDVIEAMAAYRNICNYIHLPVQSGSDRILKKMNRLHTREEYFT 322
Query: 282 IVNLLRDKIEDVSITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAE- 340
+++ ++ I + I+ D+I GFP ++ + ++F YS R GT AE
Sbjct: 323 LIDNIKKMIPNCGISHDLITGFPTETEEDHQDTLSLMEYVKYDFGYMFTYSERPGTTAER 382
Query: 341 NMENQVDGNKKDERSKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYT-RN 399
+E+ + K R +++++L + + K IG ++VL E+E + + ++G RN
Sbjct: 383 KLEDDIPEETKKRRLQEIVDLQQKHSKQNTNSVIGTTVEVLIEKESKKSNKHWSGRNERN 442
Query: 400 YIKVNAKADCNVSGKILNVKINSFEGEVAEGE 431
+ V +K + + G + VKI+ GE
Sbjct: 443 TVTVFSKENYQI-GDFVKVKIHDCTSATLIGE 473
>MIAB_RHOBA RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 479
Score = 189 bits (480), Expect = 4e-50
Identities = 126/401 (31%), Positives = 201/401 (50%), Gaps = 23/401 (5%)
Query: 3 VAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIGR 62
V T+GC++N +SE + R GY VVD AD+ + NTCS+ ++K+ +G+
Sbjct: 8 VYIKTVGCQMNVLDSEMVIADLKRHGYTVVDTPGEADLLLYNTCSIREQAEEKTYSALGK 67
Query: 63 ARR---QNPEAIIAVVGCYAQI-APTEVSGIEGVDVVLGSRNKGEIVYYVNKA------- 111
+ ++PE I V+GC AQ T VD+V+G I + K
Sbjct: 68 LKETKARHPEKTIGVMGCMAQKDQETIFRRAPFVDMVVGPGQLHAIPDMLTKVTSGEGRQ 127
Query: 112 ------RDEGKQQVKVGAVLKNKVFEDLKIEEYN-NKTRAFLKIQDGCNRFCAYCLIPYT 164
R +GKQ V ++ F+ L+ +A+L+IQ GC++FC YC++P T
Sbjct: 128 MAVSLGRKDGKQTV---VARSHETFDPLRDPTMRPTPFQAYLRIQIGCDKFCTYCVVPNT 184
Query: 165 RGSVCSKDPKKVLDEIRSLAEHGFKEIILSGIHTASYGV-DLDEKVTLVDLLEEIEKIDG 223
RG + P++++ E R LAE G EI L G SY D + + LLE + IDG
Sbjct: 185 RGPEQGRSPEEIVSEARVLAEQGALEITLLGQTVNSYRHRGPDGETDMAGLLERLHDIDG 244
Query: 224 IERVRIGSIDPTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIV 283
++R++ + P T ++ I L K+ P+ H+ QSG DA LKRM R YT +Y ++
Sbjct: 245 LKRIKFVTNYPKDMTARLLETIRDLPKVSPYLHVPAQSGSDAVLKRMKRGYTIADYMEMF 304
Query: 284 NLLRDKIEDVSITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTR-AENM 342
+ + + S+++D IVGF G ++R + IF+YS R+GT+ A N+
Sbjct: 305 ERIETVLPEASVSSDFIVGFCGETDEDFQKSVKLIERCRFKNSFIFQYSVREGTKAAANL 364
Query: 343 ENQVDGNKKDERSKKLIELNRINEKAFAEKYIGEVIDVLFE 383
+ V K R+ +L+ + K +K IG+ ++VL E
Sbjct: 365 IDDVPREVKAARNNELLAVQDRISKEDNQKLIGDTVEVLVE 405
>MIAB_LAWIP RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 457
Score = 189 bits (480), Expect = 4e-50
Identities = 115/393 (29%), Positives = 195/393 (49%), Gaps = 16/393 (4%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIGRAR-- 64
T GC++NT +S ++ ++G++ V + A + ++NTCSV ++K I+G+ R
Sbjct: 10 TFGCQMNTNDSFWLSCSLQKKGFQEVSLED-ASIIIINTCSVREKPEQKVYSILGKIRHA 68
Query: 65 -RQNPEAIIAVVGCYAQ-IAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVKV- 121
+ NP++ + + GC AQ + T V +V GS + + E ++ +
Sbjct: 69 TKNNPDSFVVIAGCVAQQLGATFFEKFPQVRLVSGSDGIAMAPDAIERLYAEPDLKLNLT 128
Query: 122 --GAVLKNKVFEDLKIEEYNNKT---RAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
+ + K+ NN T A++ I GC+ +C YC++PYTRG S+ + +
Sbjct: 129 DFSEYYPEREYAFSKLTLSNNNTLALMAYVNIMQGCDNYCTYCIVPYTRGKQKSRSVQAI 188
Query: 177 LDEIRSLAEHGFKEIILSGIHTASYGVDLDEK-----VTLVDLLEEIEKIDGIERVRIGS 231
++E + L + G KEI+L G + +YG+D D+ V L+ I + G+ER+R S
Sbjct: 189 VEECQQLIDSGVKEIVLLGQNVNAYGLDKDKNSPANGVNFAMLVHTIASLPGLERLRFFS 248
Query: 232 IDPTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIE 291
P F+ +++ LCP HL LQSG D L+RM R+Y+ EY I+ L+
Sbjct: 249 AHPKEFSSELIDLFGEFSTLCPRLHLPLQSGSDKILRRMGRKYSMDEYISIITKLKKVRP 308
Query: 292 DVSITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKK 351
D++++TD IVGFPG + I + F YS R GTR+ N+VD K
Sbjct: 309 DIALSTDFIVGFPGETEEDFLQTLQSINTIKFMSSFSFCYSDRPGTRSSTFSNKVDHEVK 368
Query: 352 DERSKKLIELNRINEKAFAEKYIGEVIDVLFEE 384
+R ++L + ++ + +G VL E+
Sbjct: 369 IKRLEQLQATQLEHSTSWLKSRVGVETTVLLEK 401
>MIAB_STRAW RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 508
Score = 189 bits (479), Expect = 5e-50
Identities = 122/384 (31%), Positives = 187/384 (48%), Gaps = 8/384 (2%)
Query: 7 TLGCRVNTYESEAMTEKFVREGY-EVVDFNEM-ADVYVVNTCSVTNMGDKKSRQIIGRA- 63
T GC++N ++SE ++ GY D ++ ADV V NTC+V D + +GR
Sbjct: 19 TYGCQMNVHDSERLSGLLEDAGYVRAPDGSDGDADVVVFNTCAVRENADNRLYGNLGRLA 78
Query: 64 --RRQNPEAIIAVVGCYAQI-APTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVK 120
+ + P IAV GC AQ T V+ VDVV G+ N G++ + +AR + + QV+
Sbjct: 79 PMKTKRPGMQIAVGGCLAQKDRDTIVTKAPWVDVVFGTHNIGKLPVLLERARVQEEAQVE 138
Query: 121 VGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDEI 180
+ L+ F + A++ I GCN C +C++P RG + +L EI
Sbjct: 139 IAESLE--AFPSTLPTRRESAYAAWVSISVGCNNTCTFCIVPALRGKEKDRRTGDILAEI 196
Query: 181 RSLAEHGFKEIILSGIHTASYGVDLDEKVTLVDLLEEIEKIDGIERVRIGSIDPTFFTED 240
+L G EI L G + +YG D+ ++ LL +IDG+ERVR S P FT+D
Sbjct: 197 EALVGEGVSEITLLGQNVNAYGSDIGDREAFSKLLRACGQIDGLERVRFTSPHPRDFTDD 256
Query: 241 VVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITTDVI 300
V+ + + P H+ +QSG D LK M R Y Y I+ +R I +ITTD+I
Sbjct: 257 VIAAMAETPNVMPQLHMPMQSGSDTVLKAMRRSYRQDRYLGIIEKVRAAIPHAAITTDII 316
Query: 301 VGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSKKLIE 360
VGFPG ++ ++ F+YS R GT A MENQ+ R ++L+
Sbjct: 317 VGFPGETEEDFEQTMHAVREARFTQAFTFQYSKRPGTPAATMENQIPKEVVQARYERLVA 376
Query: 361 LNRINEKAFAEKYIGEVIDVLFEE 384
L +K +G ++++ E
Sbjct: 377 LQEEISWDENKKQVGRTLELMVAE 400
>MIAB_RENSM RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 515
Score = 189 bits (479), Expect = 5e-50
Identities = 125/389 (32%), Positives = 192/389 (49%), Gaps = 11/389 (2%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIGR---A 63
T GC++N ++SE M R G + ++ADV V NTC+V D K +G A
Sbjct: 27 TYGCQMNVHDSERMAGLLERSGLVKAE-GKVADVVVFNTCAVRENADNKLYGNLGMLAPA 85
Query: 64 RRQNPEAIIAVVGCYAQI-APTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVKVG 122
+ NP IAV GC AQ T + VDVV G+ N G + + +AR + Q+++
Sbjct: 86 KEANPGMQIAVGGCLAQKDRDTILKRAPWVDVVFGTHNVGALPTLLERARHNEEAQLEIL 145
Query: 123 AVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDEIRS 182
L VF + + ++ I GCN C +C++P RG + P +L EI++
Sbjct: 146 ESLD--VFPSTLPTKRDAVYSGWVSISVGCNNTCTFCIVPSLRGKEKDRRPGDILAEIQA 203
Query: 183 LAEHGFKEIILSGIHTASYGVDLDEKVTLVDLLEEIEKIDGIERVRIGSIDPTFFTEDVV 242
L E G E+ L G + SYGV+ ++ LL +I+G+ERVR S P FTEDV+
Sbjct: 204 LVEDGAIEVTLLGQNVNSYGVEFGDRQAFSKLLRACGEIEGLERVRFTSPHPAAFTEDVI 263
Query: 243 RRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITTDVIVG 302
+ + P H+ LQSG D LK M R Y + ++ I++ +R +I D +I+TD+IVG
Sbjct: 264 EAMAETHNVMPQLHMPLQSGSDKVLKDMRRSYRSSKFLGILDKVRTQIPDAAISTDIIVG 323
Query: 303 FPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSKKLIELN 362
FPG + + + F+YS R GT A ++ +Q+ ER ++L L
Sbjct: 324 FPGETEEDFQATLDVVAKSRFATAFTFQYSKRPGTPAADLPDQLPKAVVQERFERLTALQ 383
Query: 363 RINEKAFAEKYIGEVIDVLFEEEVELGSG 391
+ IG ++++ V GSG
Sbjct: 384 DQISAEENARQIGREVELM----VTAGSG 408
>MIAB_CAMFF RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 434
Score = 189 bits (479), Expect = 5e-50
Identities = 144/440 (32%), Positives = 207/440 (47%), Gaps = 19/440 (4%)
Query: 2 KVAFATLGCRVNTYESE-AMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ TLGC +N +SE + E +E YEV + E AD+ ++NTCSV K +
Sbjct: 6 KLFIETLGCAMNVRDSEHIIAELSQKENYEVTNIIEDADLILINTCSVREKPVHKLFSEV 65
Query: 61 GRARRQNPE-AIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQ 118
G + + A I V GC A +EV VD VLG+RN +I +N +
Sbjct: 66 GTYEKAKKKGAKIGVCGCTASHLGSEVFKRAPYVDFVLGARNISKITTALNTPKFVS--- 122
Query: 119 VKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLD 178
V + F D + Y ++F+ I GC++ C YC++P TRG S +L
Sbjct: 123 VDINHDESEYAFGDFRSSPY----KSFVNIMIGCDKKCTYCIVPQTRGDEISIPKDIILK 178
Query: 179 EIRSLAEHGFKEIILSGIHTASYGVDLD---EKVTLVDLLEEIEKIDGIERVRIGSIDPT 235
E+R A G KEI L G + +YG EK+ DLL I ++DG+ER+R S P
Sbjct: 179 EVRKAASSGAKEIFLLGQNVNNYGKRFSGTHEKIDFSDLLNLISEVDGVERIRFTSPHPL 238
Query: 236 FFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSI 295
+ ++ K+C H+ LQSG + LK M R YT + + D LR+ DV+I
Sbjct: 239 HMDDKFLKEFSTNPKICKSMHMPLQSGSNEILKAMKRGYTKEWFLDRALKLREICSDVTI 298
Query: 296 TTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERS 355
+TD+IV FPG ++ I + FKYS R T A +M NQV+ D S
Sbjct: 299 STDIIVAFPGESDRDFADTMSVVESIKFEQMFSFKYSSRPLTPAASMTNQVN---SDIAS 355
Query: 356 KKLIELNRINEKAFAE--KYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSG 413
++L L + + E K I ++ EE+ S V G T N V + G
Sbjct: 356 RRLSTLQARHTEILDEIVKAQKNSIHEVYFEELRANSSV-AGRTFNNFLVQTNGSEELLG 414
Query: 414 KILNVKINSFEGEVAEGEIV 433
KIL VKI + V G+I+
Sbjct: 415 KILKVKITDPKRMVLYGQII 434
>MIAB_PSESM RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 442
Score = 188 bits (478), Expect = 7e-50
Identities = 134/445 (30%), Positives = 218/445 (48%), Gaps = 20/445 (4%)
Query: 2 KVAFATLGCRVNTYESEAMTEKF-VREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S M + + EV E ADV ++NTCS+ + +
Sbjct: 4 KLYIETHGCQMNEYDSSRMVDLLGEHQALEVTARAEDADVILLNTCSIRERAQDRVYSQL 63
Query: 61 GRARR---QNPEAIIAVVGCYAQIAPTEVSGIEG----VDVVLGSRNKGEIVYYVNKARD 113
GR R NPE +IAV GC +A E + I VDVV G + + ++ AR
Sbjct: 64 GRWRELKLANPEMVIAVGGC---VASQEGAAIRDRAPYVDVVFGPQTLHRLPEMIDAARI 120
Query: 114 EGKQQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDP 173
QV V K F+ L + + A++ + +GC+++C +C++PYTRG S+
Sbjct: 121 TRLPQVDVSFPEIEK-FDHLPEPRVDGPS-AYVSVMEGCSKYCTFCVVPYTRGEEVSRPF 178
Query: 174 KKVLDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKVT-LVDLLEEIEKIDGIERVRIGS 231
VL E+ LAEHG +E+ L G + Y G D +V L DL+ + +DGI+R+R +
Sbjct: 179 DDVLTEVTHLAEHGVREVTLLGQNVNGYRGTTHDGRVADLADLIRAVAVVDGIDRIRYTT 238
Query: 232 IDPTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIE 291
P F++ +++ + +L H HL +QSG D L M R +T EY+ + LR +
Sbjct: 239 SHPLEFSDSLIQAHADVPELVKHLHLPVQSGSDRILAAMKRNHTTLEYKSRLRKLRAAVP 298
Query: 292 DVSITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKK 351
+SI++D IVGFPG ++ + F YSPR GT A ++++ K
Sbjct: 299 GISISSDFIVGFPGETEKDFDNTMKLIEDAGFDFSFSFIYSPRPGTPAADLKDDTPEALK 358
Query: 352 DERSKKLIELNRINEKAF--AEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKAD- 408
ER L +R+N++ F + + +G + +L + + G G T N VN + D
Sbjct: 359 KERLAAL--QHRLNQQGFEISRQMVGSIQRILVTDYSKKDPGELQGRTENNRIVNFRCDN 416
Query: 409 CNVSGKILNVKINSFEGEVAEGEIV 433
+ G+ +V I+ + G ++
Sbjct: 417 PRLIGQFADVHIDDAQPHSLRGSLL 441
>MIAB_SERP5 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 474
Score = 188 bits (477), Expect = 9e-50
Identities = 140/445 (31%), Positives = 214/445 (48%), Gaps = 20/445 (4%)
Query: 2 KVAFATLGCRVNTYESEAMTEKF-VREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S M + GY+ D E ADV ++NTCS+ +K ++
Sbjct: 4 KLHIKTWGCQMNEYDSSKMADLLGSTHGYQWTDNAEEADVLLLNTCSIREKAQEKVFAML 63
Query: 61 GRAR---RQNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR R +NP II V GC A + S VDVV G + + +N +
Sbjct: 64 GRWRLLKEKNPALIIGVGGCVASQEGEHIRSRAPCVDVVFGPQTLHRLPEMLNHVQGTRS 123
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
V + K F+ L T AF+ I +GCN++C +C++PYTRG S+ V
Sbjct: 124 PIVDISFPEIEK-FDRLPEPRAEGPT-AFVSIMEGCNKYCTFCVVPYTRGEEVSRPSDDV 181
Query: 177 LDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKV-TLVDLLEEIEKIDGIERVRIGSIDP 234
L E+ LA G +E+ L G + +Y G D + + +LL + IDGI+R+R + P
Sbjct: 182 LFEVAQLAAQGVREVNLLGQNVNAYRGATYDGGICSFAELLRLVAAIDGIDRIRYTTSHP 241
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
FT+D++ +L HL +QSG D L M R +TA EY+ I+ LR D+
Sbjct: 242 IEFTDDIIAVYEDTPELVSFLHLPVQSGSDRILTMMKRAHTALEYKAIIRKLRKARPDIQ 301
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
+++D I+GFPG + I + F YS R GT A +M VD +DE+
Sbjct: 302 LSSDFIIGFPGETQADFEQTMNLIAEINFDTSFSFIYSSRPGTPAADM---VDDVSEDEK 358
Query: 355 SKKLIEL-NRINEKA--FAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNV 411
++L L +RIN++ F+ + +G V +L E G T VN + ++
Sbjct: 359 KQRLYILQDRINQQVLQFSRRMLGTVQRILVEGTSRKSVMELAGRTACNRMVNFEGTPDM 418
Query: 412 SGKILNVKI-----NSFEGEVAEGE 431
G+ ++V+I NS G V E
Sbjct: 419 IGQFVDVEITEVLTNSLRGTVVRTE 443
>MIAB_SALAI RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 502
Score = 188 bits (477), Expect = 9e-50
Identities = 129/434 (29%), Positives = 204/434 (47%), Gaps = 21/434 (4%)
Query: 7 TLGCRVNTYESEAMTEKFVREGY-EVVDFNEMADVYVVNTCSVTNMGDKKSRQIIGRARR 65
T GC++N ++SE + + GY NTC+V D + +G+ R
Sbjct: 18 TYGCQMNVHDSERIAGLLEQAGYLRAGXXXXXXXXXXFNTCAVRENADNRLYGNLGQLRP 77
Query: 66 ---QNPEAIIAVVGCYAQIAPTE-VSGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVKV 121
++P IAV GC AQ +E V VDVV G+ N G + + +AR +V++
Sbjct: 78 SKDRHPGMQIAVGGCLAQKDRSEIVRRAPWVDVVFGTHNIGSLPVLLERARHNAAAEVEI 137
Query: 122 GAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDEIR 181
L VF + ++ I GCN C +C++P RG + P VL E+R
Sbjct: 138 LESLD--VFPSTLPTRRESTYAGWVSISVGCNNTCTFCIVPALRGKERDRRPGDVLSEVR 195
Query: 182 SLAEHGFKEIILSGIHTASYGVDLDEKVTLVDLLEEIEKIDGIERVRIGSIDPTFFTEDV 241
+L + G E+ L G + SYG++ ++ LL IDG+ERVR S P FT+DV
Sbjct: 196 ALVDEGVLEVTLLGQNVNSYGIEFGDRYAFGKLLRACGDIDGLERVRFTSPHPKDFTDDV 255
Query: 242 VRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITTDVIV 301
+ + +C H+ LQSG D L+ M R Y ++ Y I+ +R + D +ITTD+IV
Sbjct: 256 IAAMAETPNVCHSLHMPLQSGSDDVLRAMRRSYRSERYLGIIEKVRAAMPDAAITTDIIV 315
Query: 302 GFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSKKLIEL 361
GFPG ++ S F+YS R GT A M +Q+ ER ++L+
Sbjct: 316 GFPGETEADFERTLDVVRSARFSSAFTFQYSKRPGTPAATMADQLPKPVVQERYERLVAC 375
Query: 362 NRINEKAFAE--KYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSGKILNVK 419
+ E +AE + +GE ++VL V +G G R + + G++++
Sbjct: 376 --VEEITWAENRRLVGETVEVL----VAVGEG------RKDERTGRLSGRARDGRLVHFD 423
Query: 420 INSFEGEVAEGEIV 433
S G++ G++V
Sbjct: 424 AGSLAGQIRPGDVV 437
>MIAB_RALEH RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 450
Score = 188 bits (477), Expect = 9e-50
Identities = 138/449 (30%), Positives = 211/449 (46%), Gaps = 19/449 (4%)
Query: 2 KVAFATLGCRVNTYESEAMTEKF-VREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
KV T GC++N Y+S+ M + +G E D E ADV + NTCSV +K +
Sbjct: 3 KVFVKTYGCQMNEYDSDKMVDVLNASQGLEPTDNVEEADVILFNTCSVREKAQEKVFSEL 62
Query: 61 GRARRQN---PEAIIAVVGCYA-QIAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR + P+ +I V GC A Q + VS VDVV G + + + + + G+
Sbjct: 63 GRMKALKAVKPDLVIGVGGCVASQEGASIVSRAPYVDVVFGPQTLHRLPDLIARRQRTGQ 122
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
QV + K F+ L + AF+ I +GC+++C+YC++PYTRG S+ + V
Sbjct: 123 SQVDISFPEIEK-FDHLPPARVEGPS-AFVSIMEGCSKYCSYCVVPYTRGEEVSRPFEDV 180
Query: 177 LDEIRSLAEHGFKEIILSGIHTASYGVDLDEKVTLVD---LLEEIEKIDGIERVRIGSID 233
L E+ LAE G +E+ L G + +Y + + + D L+E + +I GIER+R +
Sbjct: 181 LAEVAGLAEQGVREVTLLGQNVNAYRGKMGDTTEIADFALLIEYVAEIPGIERIRYTTSH 240
Query: 234 PTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDV 293
P FT +V KL H HL +Q D L M R Y+ EY+ I+ LR D+
Sbjct: 241 PKEFTSRLVELYGRCDKLVNHLHLPVQHASDRILMAMKRGYSVLEYKSIIRKLRVLRPDM 300
Query: 294 SITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDE 353
S+++D IVGFPG ++ I + F +SPR GT A N+ + K
Sbjct: 301 SMSSDFIVGFPGETDADFEKLMAMIEEIGYDTSFSFIFSPRPGTPAANLHDDTPHEVKLR 360
Query: 354 RSKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVN--------A 405
R ++L N + ++ +G V +L E G T N VN A
Sbjct: 361 RLQRLQATIEENVQRISQNMVGTVQRILVEGPARKDPTELHGRTENNRVVNFALPGVPQA 420
Query: 406 KADCNVSGKILNVKINSFEGEVAEGEIVL 434
D + G++++V I GEIV+
Sbjct: 421 GRD-RLVGQLVDVSITQAFPHSLRGEIVV 448
>MIAB_MYCTU RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 512
Score = 188 bits (477), Expect = 9e-50
Identities = 121/385 (31%), Positives = 177/385 (45%), Gaps = 12/385 (3%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKK---SRQIIGRA 63
T GC++N ++SE + GY ADV V NTC+V D + + +
Sbjct: 31 TYGCQMNVHDSERLAGLLEAAGYRRATDGSEADVVVFNTCAVRENADNRLYGNLSHLAPR 90
Query: 64 RRQNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVKVG 122
+R NP+ IAV GC AQ V VDVV G+ N G + + +AR QV++
Sbjct: 91 KRANPDMQIAVGGCLAQKDRDAVLRRAPWVDVVFGTHNIGSLPTLLERARHNKVAQVEIA 150
Query: 123 AVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDEIRS 182
L+ F + A++ I GCN C +C++P RG + P +L E+RS
Sbjct: 151 EALQQ--FPSSLPSSRESAYAAWVSISVGCNNSCTFCIVPSLRGREVDRSPADILAEVRS 208
Query: 183 LAEHGFKEIILSGIHTASYGVDLDEKV------TLVDLLEEIEKIDGIERVRIGSIDPTF 236
L G E+ L G + +YGV + +LL IDG+ERVR S P
Sbjct: 209 LVNDGVLEVTLLGQNVNAYGVSFADPALPRNRGAFAELLRACGDIDGLERVRFTSPHPAE 268
Query: 237 FTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSIT 296
FT+DV+ + + +CP H+ LQSG D L+ M R Y A+ Y I+ +R I +IT
Sbjct: 269 FTDDVIEAMAQTRNVCPALHMPLQSGSDRILRAMRRSYRAERYLGIIERVRAAIPHAAIT 328
Query: 297 TDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSK 356
TD+IVGFPG + +YS R GT A ++ Q+ ER +
Sbjct: 329 TDLIVGFPGETEEDFAATLDVVXXXXXXXXXXXQYSKRPGTPAAQLDGQLPKAVVQERYE 388
Query: 357 KLIELNRINEKAFAEKYIGEVIDVL 381
+LI L +G+ ++VL
Sbjct: 389 RLIALQEQISLEANRALVGQAVEVL 413
>MIAB_MYCTA RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 512
Score = 188 bits (477), Expect = 9e-50
Identities = 121/385 (31%), Positives = 177/385 (45%), Gaps = 12/385 (3%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKK---SRQIIGRA 63
T GC++N ++SE + GY ADV V NTC+V D + + +
Sbjct: 31 TYGCQMNVHDSERLAGLLEAAGYRRATDGSEADVVVFNTCAVRENADNRLYGNLSHLAPR 90
Query: 64 RRQNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVKVG 122
+R NP+ IAV GC AQ V VDVV G+ N G + + +AR QV++
Sbjct: 91 KRANPDMQIAVGGCLAQKDRDAVLRRAPWVDVVFGTHNIGSLPTLLERARHNKVAQVEIA 150
Query: 123 AVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDEIRS 182
L+ F + A++ I GCN C +C++P RG + P +L E+RS
Sbjct: 151 EALQQ--FPSSLPSSRESAYAAWVSISVGCNNSCTFCIVPSLRGREVDRSPADILAEVRS 208
Query: 183 LAEHGFKEIILSGIHTASYGVDLDEKV------TLVDLLEEIEKIDGIERVRIGSIDPTF 236
L G E+ L G + +YGV + +LL IDG+ERVR S P
Sbjct: 209 LVNDGVLEVTLLGQNVNAYGVSFADPALPRNRGAFAELLRACGDIDGLERVRFTSPHPAE 268
Query: 237 FTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSIT 296
FT+DV+ + + +CP H+ LQSG D L+ M R Y A+ Y I+ +R I +IT
Sbjct: 269 FTDDVIEAMAQTRNVCPALHMPLQSGSDRILRAMRRSYRAERYLGIIERVRAAIPHAAIT 328
Query: 297 TDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSK 356
TD+IVGFPG + +YS R GT A ++ Q+ ER +
Sbjct: 329 TDLIVGFPGETEEDFAATLDVVXXXXXXXXXXXQYSKRPGTPAAQLDGQLPKAVVQERYE 388
Query: 357 KLIELNRINEKAFAEKYIGEVIDVL 381
+LI L +G+ ++VL
Sbjct: 389 RLIALQEQISLEANRALVGQAVEVL 413
>MIAB_MYCBP RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 512
Score = 188 bits (477), Expect = 9e-50
Identities = 121/385 (31%), Positives = 177/385 (45%), Gaps = 12/385 (3%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKK---SRQIIGRA 63
T GC++N ++SE + GY ADV V NTC+V D + + +
Sbjct: 31 TYGCQMNVHDSERLAGLLEAAGYRRATDGSEADVVVFNTCAVRENADNRLYGNLSHLAPR 90
Query: 64 RRQNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVKVG 122
+R NP+ IAV GC AQ V VDVV G+ N G + + +AR QV++
Sbjct: 91 KRANPDMQIAVGGCLAQKDRDAVLRRAPWVDVVFGTHNIGSLPTLLERARHNKVAQVEIA 150
Query: 123 AVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDEIRS 182
L+ F + A++ I GCN C +C++P RG + P +L E+RS
Sbjct: 151 EALQQ--FPSSLPSSRESAYAAWVSISVGCNNSCTFCIVPSLRGREVDRSPADILAEVRS 208
Query: 183 LAEHGFKEIILSGIHTASYGVDLDEKV------TLVDLLEEIEKIDGIERVRIGSIDPTF 236
L G E+ L G + +YGV + +LL IDG+ERVR S P
Sbjct: 209 LVNDGVLEVTLLGQNVNAYGVSFADPALPRNRGAFAELLRACGDIDGLERVRFTSPHPAE 268
Query: 237 FTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSIT 296
FT+DV+ + + +CP H+ LQSG D L+ M R Y A+ Y I+ +R I +IT
Sbjct: 269 FTDDVIEAMAQTRNVCPALHMPLQSGSDRILRAMRRSYRAERYLGIIERVRAAIPHAAIT 328
Query: 297 TDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSK 356
TD+IVGFPG + +YS R GT A ++ Q+ ER +
Sbjct: 329 TDLIVGFPGETEEDFAATLDVVXXXXXXXXXXXQYSKRPGTPAAQLDGQLPKAVVQERYE 388
Query: 357 KLIELNRINEKAFAEKYIGEVIDVL 381
+LI L +G+ ++VL
Sbjct: 389 RLIALQEQISLEANRALVGQAVEVL 413
>MIAB_MYCBO RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 512
Score = 188 bits (477), Expect = 9e-50
Identities = 121/385 (31%), Positives = 177/385 (45%), Gaps = 12/385 (3%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKK---SRQIIGRA 63
T GC++N ++SE + GY ADV V NTC+V D + + +
Sbjct: 31 TYGCQMNVHDSERLAGLLEAAGYRRATDGSEADVVVFNTCAVRENADNRLYGNLSHLAPR 90
Query: 64 RRQNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVKVG 122
+R NP+ IAV GC AQ V VDVV G+ N G + + +AR QV++
Sbjct: 91 KRANPDMQIAVGGCLAQKDRDAVLRRAPWVDVVFGTHNIGSLPTLLERARHNKVAQVEIA 150
Query: 123 AVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDEIRS 182
L+ F + A++ I GCN C +C++P RG + P +L E+RS
Sbjct: 151 EALQQ--FPSSLPSSRESAYAAWVSISVGCNNSCTFCIVPSLRGREVDRSPADILAEVRS 208
Query: 183 LAEHGFKEIILSGIHTASYGVDLDEKV------TLVDLLEEIEKIDGIERVRIGSIDPTF 236
L G E+ L G + +YGV + +LL IDG+ERVR S P
Sbjct: 209 LVNDGVLEVTLLGQNVNAYGVSFADPALPRNRGAFAELLRACGDIDGLERVRFTSPHPAE 268
Query: 237 FTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSIT 296
FT+DV+ + + +CP H+ LQSG D L+ M R Y A+ Y I+ +R I +IT
Sbjct: 269 FTDDVIEAMAQTRNVCPALHMPLQSGSDRILRAMRRSYRAERYLGIIERVRAAIPHAAIT 328
Query: 297 TDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSK 356
TD+IVGFPG + +YS R GT A ++ Q+ ER +
Sbjct: 329 TDLIVGFPGETEEDFAATLDVVXXXXXXXXXXXQYSKRPGTPAAQLDGQLPKAVVQERYE 388
Query: 357 KLIELNRINEKAFAEKYIGEVIDVL 381
+LI L +G+ ++VL
Sbjct: 389 RLIALQEQISLEANRALVGQAVEVL 413
>MIAB_MICAN RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 446
Score = 188 bits (477), Expect = 9e-50
Identities = 120/426 (28%), Positives = 205/426 (48%), Gaps = 16/426 (3%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIGRARRQ 66
T GC++N +SE M G++ + AD+ + NTC++ + ++K +GR ++
Sbjct: 12 TFGCQMNKADSERMAGILEDLGFQWSEDANEADLILYNTCTIRDNAEQKVYSYLGRQAKR 71
Query: 67 N---PEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVKVG 122
P+ + V GC AQ ++ + VD+++G ++ + + + D G Q V
Sbjct: 72 KQTQPDLTLIVAGCVAQQEGEQLLRRVPEVDLIIGPQHANRLGDLLQQVFD-GSQVVATE 130
Query: 123 AVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDEIRS 182
+ + ED+ ++ A++ + GCN C YC++P RG S+ P + E+
Sbjct: 131 PI---HIMEDITKPRRDSNITAWVNVIYGCNERCTYCVVPGVRGVEQSRTPAAIRAEMAQ 187
Query: 183 LAEHGFKEIILSGIHTASYGVDLDEKVT-------LVDLLEEIEKIDGIERVRIGSIDPT 235
L + G++EI L G + +YG DL DLL + + GIER+R + P
Sbjct: 188 LGQQGYQEITLLGQNIDAYGRDLPGVTASGRHLHNFTDLLYYVHDVAGIERLRFATSHPR 247
Query: 236 FFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSI 295
+FTE +++ L K+C HFH+ QSG + LK M R YT ++Y I+ +RD + D +I
Sbjct: 248 YFTERLIKACQELPKVCEHFHIPFQSGDNDILKAMKRGYTQEKYRQIIANIRDLMPDAAI 307
Query: 296 TTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERS 355
+ D IVGFPG + I + + YSPR GT A ++Q+ K +R
Sbjct: 308 SADAIVGFPGETEAQFENTLKLVDEIGFDQLNTAAYSPRPGTPAAIWDDQLSEQVKSDRL 367
Query: 356 KKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADC-NVSGK 414
++L L +++Y+G + ++L E+ + G TR D + GK
Sbjct: 368 QRLNHLVATKAAERSQRYLGRIEEILVEDVNPKDASQVMGRTRGNRLTFFTGDIEELRGK 427
Query: 415 ILNVKI 420
+ VKI
Sbjct: 428 FVKVKI 433
>MIAB_LEGPL RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 447
Score = 188 bits (477), Expect = 9e-50
Identities = 134/443 (30%), Positives = 210/443 (47%), Gaps = 14/443 (3%)
Query: 2 KVAFATLGCRVNTYESEAMTEK-FVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S M E + G D E ADV ++NTCS+ +K +
Sbjct: 4 KLYIKTNGCQMNEYDSSKMAEVLYASHGLVKTDQVEDADVILLNTCSIREKAQEKVFSQL 63
Query: 61 GRARR---QNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
G+ R +NP +I V GC A +++ VD+V G + + +N+ ++ K
Sbjct: 64 GQWREYKAKNPHVLIGVGGCVASQEGSDIIKRAPFVDIVFGPQTLHRLPALLNERLEKNK 123
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
V + K F+ L T AF+ I +GC+++C++C++PYTRG S+ V
Sbjct: 124 SVVDISFPEIEK-FDHLPAPRAEGPT-AFVSIMEGCSKYCSFCVVPYTRGEEISRPFDDV 181
Query: 177 LDEIRSLAEHGFKEIILSGIHTASYG--VDLDEKVTLVDLLEEIEKIDGIERVRIGSIDP 234
L E LA G +EI L G + Y +D + L L+ I IDGI R+R + P
Sbjct: 182 LAECYQLASQGVREINLLGQNVNDYRGIMDNGDIADLALLIHYIAAIDGIGRIRFTTSHP 241
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
F+E+++ + +L H HL +QSG D L M R YTA E++ + LR D+
Sbjct: 242 LAFSENLINAYAEVPELANHLHLPVQSGSDRILSLMKRGYTALEFKSKIRKLRKIRPDIR 301
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
++TD+IVGFPG + I + F YSPR GT A N+ + K +R
Sbjct: 302 LSTDIIVGFPGETDKDFQDTMDLVHEIGFDTSFSFIYSPRPGTPAANLPDDTPMEIKKQR 361
Query: 355 SKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSGK 414
+ L +N ++E IG +L + S +G T VN ++ G+
Sbjct: 362 LQILQNRLLMNAARYSESMIGSKQKILVTGFSKKSSQQLSGRTECNRVVNFDGPPHLIGQ 421
Query: 415 ILNVKI-----NSFEGEVAEGEI 432
++V+I NS G + E E+
Sbjct: 422 FIDVQISDALPNSLRGRLLEKEM 444
>MIAB_LEGPH RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 447
Score = 188 bits (477), Expect = 9e-50
Identities = 134/443 (30%), Positives = 210/443 (47%), Gaps = 14/443 (3%)
Query: 2 KVAFATLGCRVNTYESEAMTEK-FVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S M E + G D E ADV ++NTCS+ +K +
Sbjct: 4 KLYIKTNGCQMNEYDSSKMAEVLYASHGLVKTDQVEDADVILLNTCSIREKAQEKVFSQL 63
Query: 61 GRARR---QNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
G+ R +NP +I V GC A +++ VD+V G + + +N+ ++ K
Sbjct: 64 GQWREYKAKNPHVLIGVGGCVASQEGSDIIKRAPFVDIVFGPQTLHRLPALLNERLEKNK 123
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
V + K F+ L T AF+ I +GC+++C++C++PYTRG S+ V
Sbjct: 124 SVVDISFPEIEK-FDHLPAPRAEGPT-AFVSIMEGCSKYCSFCVVPYTRGEEISRPFDDV 181
Query: 177 LDEIRSLAEHGFKEIILSGIHTASYG--VDLDEKVTLVDLLEEIEKIDGIERVRIGSIDP 234
L E LA G +EI L G + Y +D + L L+ I IDGI R+R + P
Sbjct: 182 LAECYQLASQGVREINLLGQNVNDYRGIMDNGDIADLALLIHYIAAIDGIGRIRFTTSHP 241
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
F+E+++ + +L H HL +QSG D L M R YTA E++ + LR D+
Sbjct: 242 LAFSENLINAYAEVPELANHLHLPVQSGSDRILSLMKRGYTALEFKSKIRKLRKIRPDIR 301
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
++TD+IVGFPG + I + F YSPR GT A N+ + K +R
Sbjct: 302 LSTDIIVGFPGETDKDFQDTMDLVHEIGFDTSFSFIYSPRPGTPAANLPDDTPMEIKKQR 361
Query: 355 SKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSGK 414
+ L +N ++E IG +L + S +G T VN ++ G+
Sbjct: 362 LQILQNRLLMNAARYSESMIGSKQKILVTGFSKKSSQQLSGRTECNRVVNFDGPPHLIGQ 421
Query: 415 ILNVKI-----NSFEGEVAEGEI 432
++V+I NS G + E E+
Sbjct: 422 FIDVQISDALPNSLRGRLLEKEM 444
>MIAB_LEGPC RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 447
Score = 188 bits (477), Expect = 9e-50
Identities = 134/443 (30%), Positives = 210/443 (47%), Gaps = 14/443 (3%)
Query: 2 KVAFATLGCRVNTYESEAMTEK-FVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S M E + G D E ADV ++NTCS+ +K +
Sbjct: 4 KLYIKTNGCQMNEYDSSKMAEVLYASHGLVKTDQVEDADVILLNTCSIREKAQEKVFSQL 63
Query: 61 GRARR---QNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
G+ R +NP +I V GC A +++ VD+V G + + +N+ ++ K
Sbjct: 64 GQWREYKAKNPHVLIGVGGCVASQEGSDIIKRAPFVDIVFGPQTLHRLPALLNERLEKNK 123
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
V + K F+ L T AF+ I +GC+++C++C++PYTRG S+ V
Sbjct: 124 SVVDISFPEIEK-FDHLPAPRAEGPT-AFVSIMEGCSKYCSFCVVPYTRGEEISRPFDDV 181
Query: 177 LDEIRSLAEHGFKEIILSGIHTASYG--VDLDEKVTLVDLLEEIEKIDGIERVRIGSIDP 234
L E LA G +EI L G + Y +D + L L+ I IDGI R+R + P
Sbjct: 182 LAECYQLASQGVREINLLGQNVNDYRGIMDNGDIADLALLIHYIAAIDGIGRIRFTTSHP 241
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
F+E+++ + +L H HL +QSG D L M R YTA E++ + LR D+
Sbjct: 242 LAFSENLINAYAEVPELANHLHLPVQSGSDRILSLMKRGYTALEFKSKIRKLRKIRPDIR 301
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
++TD+IVGFPG + I + F YSPR GT A N+ + K +R
Sbjct: 302 LSTDIIVGFPGETDKDFQDTMDLVHEIGFDTSFSFIYSPRPGTPAANLPDDTPMEIKKQR 361
Query: 355 SKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSGK 414
+ L +N ++E IG +L + S +G T VN ++ G+
Sbjct: 362 LQILQNRLLMNAARYSESMIGSKQKILVTGFSKKSSQQLSGRTECNRVVNFDGPPHLIGQ 421
Query: 415 ILNVKI-----NSFEGEVAEGEI 432
++V+I NS G + E E+
Sbjct: 422 FIDVQISDALPNSLRGRLLEKEM 444
>MIAB_LEGPA RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 447
Score = 188 bits (477), Expect = 9e-50
Identities = 134/443 (30%), Positives = 210/443 (47%), Gaps = 14/443 (3%)
Query: 2 KVAFATLGCRVNTYESEAMTEK-FVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S M E + G D E ADV ++NTCS+ +K +
Sbjct: 4 KLYIKTNGCQMNEYDSSKMAEVLYASHGLVKTDQVEDADVILLNTCSIREKAQEKVFSQL 63
Query: 61 GRARR---QNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
G+ R +NP +I V GC A +++ VD+V G + + +N+ ++ K
Sbjct: 64 GQWREYKAKNPHVLIGVGGCVASQEGSDIIKRAPFVDIVFGPQTLHRLPALLNERLEKNK 123
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
V + K F+ L T AF+ I +GC+++C++C++PYTRG S+ V
Sbjct: 124 SVVDISFPEIEK-FDHLPAPRAEGPT-AFVSIMEGCSKYCSFCVVPYTRGEEISRPFDDV 181
Query: 177 LDEIRSLAEHGFKEIILSGIHTASYG--VDLDEKVTLVDLLEEIEKIDGIERVRIGSIDP 234
L E LA G +EI L G + Y +D + L L+ I IDGI R+R + P
Sbjct: 182 LAECYQLASQGVREINLLGQNVNDYRGIMDNGDIADLALLIHYIAAIDGIGRIRFTTSHP 241
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
F+E+++ + +L H HL +QSG D L M R YTA E++ + LR D+
Sbjct: 242 LAFSENLINAYAEVPELANHLHLPVQSGSDRILSLMKRGYTALEFKSKIRKLRKIRPDIR 301
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
++TD+IVGFPG + I + F YSPR GT A N+ + K +R
Sbjct: 302 LSTDIIVGFPGETDKDFQDTMDLVHEIGFDTSFSFIYSPRPGTPAANLPDDTPMEIKKQR 361
Query: 355 SKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSGK 414
+ L +N ++E IG +L + S +G T VN ++ G+
Sbjct: 362 LQILQNRLLMNAARYSESMIGSKQKILVTGFSKKSSQQLSGRTECNRVVNFDGPPHLIGQ 421
Query: 415 ILNVKI-----NSFEGEVAEGEI 432
++V+I NS G + E E+
Sbjct: 422 FIDVQISDALPNSLRGRLLEKEM 444
>MIAB_DINSH RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 437
Score = 188 bits (477), Expect = 9e-50
Identities = 130/442 (29%), Positives = 205/442 (46%), Gaps = 22/442 (4%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIG 61
K+ T GC++N Y+SE M E EGY D E AD+ ++NTC + +K +G
Sbjct: 6 KLYIKTYGCQMNVYDSERMAEAMGGEGYVQTDRAEDADMILLNTCHIREKAAEKVYSELG 65
Query: 62 R---ARRQNPEAIIAVVGCYAQIAPTEVSGIEG-VDVVLGSRNKGEIVYYVNKARDEGKQ 117
R + + P+ I V GC AQ E+ + VD+V+G + + +A K
Sbjct: 66 RFKPLKAEKPDLKIGVAGCVAQAEGAEIMRRQPLVDLVVGPQAYHRLPELTARAATGAK- 124
Query: 118 QVKVGAVLKNKVFEDLKIEEYNNKTRA------FLKIQDGCNRFCAYCLIPYTRGSVCSK 171
L E+ K + + +A FL +Q+GC++FCA+C++PYTRG+ S+
Sbjct: 125 ------ALDTDFPEEDKFDHLAARPKAKRGPTAFLTVQEGCDKFCAFCVVPYTRGAEVSR 178
Query: 172 DPKKVLDEIRSLAEHGFKEIILSGIHTASYGVDLDEKVTLVDLLEEIEKIDGIERVRIGS 231
+VL E R L E G +EI L G + +Y L L+ ++ +IDG+ER+R +
Sbjct: 179 PAARVLTEARDLVERGVREITLLGQNVNAYH---GHARGLAGLIWDLAEIDGLERIRFTT 235
Query: 232 IDPTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIE 291
P + ++ A KL P+ HL +QSG D LK MNR++TA+ Y ++ +R
Sbjct: 236 SHPNDMDDALIAAHGACDKLMPYLHLPVQSGSDRILKAMNRKHTAESYIRLIERIRAARP 295
Query: 292 DVSITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKK 351
D+ ++ D IVGFPG ++ + + + FKYS R GT A E+ V G
Sbjct: 296 DILLSGDFIVGFPGETDQDFADTMALVEAVGYGQAYSFKYSARPGTPAAEKED-VPGEVA 354
Query: 352 DERSKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNV 411
D + +G VLFE+ L G G + + V+ A
Sbjct: 355 DXXXXXXXXXXXXXXXXIQDAKVGTTARVLFEKPGRL-PGQMVGKSEHLHAVHVAAPDAA 413
Query: 412 SGKILNVKINSFEGEVAEGEIV 433
G ++ V+I G ++
Sbjct: 414 RGDLVRVRIAESSANSLRGVLI 435
>MIAB_CUPTR RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 450
Score = 188 bits (477), Expect = 9e-50
Identities = 138/449 (30%), Positives = 212/449 (47%), Gaps = 19/449 (4%)
Query: 2 KVAFATLGCRVNTYESEAMTEKF-VREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
KV T GC++N Y+S+ M + +G E D E ADV + NTCSV +K +
Sbjct: 3 KVFVKTYGCQMNEYDSDKMVDVLNASQGLEATDNVEDADVILFNTCSVREKAQEKVFSEL 62
Query: 61 GRARRQN---PEAIIAVVGCYA-QIAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR + P+ +I V GC A Q + VS VDVV G + + + + + G+
Sbjct: 63 GRMKALKAVKPDLVIGVGGCVASQEGASIVSRAPYVDVVFGPQTLHRLPDLIARRQRTGQ 122
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
QV + K F+ L + AF+ I +GC+++C+YC++PYTRG S+ + V
Sbjct: 123 SQVDISFPEIEK-FDHLPPARVEGPS-AFVSIMEGCSKYCSYCVVPYTRGEEVSRPFEDV 180
Query: 177 LDEIRSLAEHGFKEIILSGIHTASYGVDLDEKVTLVD---LLEEIEKIDGIERVRIGSID 233
L E+ LAE G +E+ L G + +Y + + + D L+E + +I GIER+R +
Sbjct: 181 LAEVAGLAEQGVREVTLLGQNVNAYRGKMGDTSEIADFALLIEYVAEIPGIERIRYTTSH 240
Query: 234 PTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDV 293
P FT +V KL H HL +Q D L M R Y+ EY+ I+ LR D+
Sbjct: 241 PKEFTSRLVELYGRCDKLVNHLHLPVQHASDRILMAMKRGYSVLEYKSIIRRLRALRPDM 300
Query: 294 SITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDE 353
S+++D IVGFPG ++ I + F +SPR GT A N+ + K +
Sbjct: 301 SMSSDFIVGFPGETDADFDKLMAMIEEIGYDTSFSFIFSPRPGTPAANLHDDTPREVKLQ 360
Query: 354 RSKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVN--------A 405
R ++L N + ++ +G V +L E G T N VN A
Sbjct: 361 RLQRLQATIEENVQRISQGMVGTVQRILVEGPARKDPTELHGRTENNRVVNFALPGVPQA 420
Query: 406 KADCNVSGKILNVKINSFEGEVAEGEIVL 434
D + G++++V I GEIV+
Sbjct: 421 GRD-RLVGQLVDVSITQAFPHSLRGEIVV 448
>MIAB_SODGM RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 474
Score = 187 bits (476), Expect = 1e-49
Identities = 136/444 (30%), Positives = 212/444 (47%), Gaps = 18/444 (4%)
Query: 2 KVAFATLGCRVNTYESEAMTEKF-VREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S M + GY++ D E ADV ++NTCS+ +K +
Sbjct: 4 KLHIKTWGCQMNEYDSSKMADLLDSTHGYQLTDNPEEADVLLLNTCSIREKAQEKVFHQL 63
Query: 61 GR---ARRQNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR + PE II V GC A + VD++ G + + +N
Sbjct: 64 GRWHMLKEARPELIIGVGGCVASQEGAHIRERANYVDIIFGPQTLHRLPEMINHVNGTRS 123
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
V + K F+ L T AF+ I +GCN++C++C++PYTRG S+ +
Sbjct: 124 PVVDISFPEIEK-FDRLPEPRAEGPT-AFVSIMEGCNKYCSFCVVPYTRGEEVSRPCDDI 181
Query: 177 LDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKV-TLVDLLEEIEKIDGIERVRIGSIDP 234
L EI LA+ G +E+ L G + +Y G D + T +LL + IDGI+R+R + P
Sbjct: 182 LFEIAQLADQGVREVNLLGQNVNAYRGASYDGGICTFAELLRLVAAIDGIDRIRYITSHP 241
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
FT+D++ +L HL +QSG D L M R +TA EY+ I+ LR D+
Sbjct: 242 IEFTDDIIDVYRDTPELVSFVHLPVQSGSDRILTMMKRAHTALEYKSIIRRLRKARPDIQ 301
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
I++D I+GFPG + + + F YS R GT A +M + V +K +R
Sbjct: 302 ISSDFIIGFPGETQQDFEQTMQLIADVNFDMSFSFIYSARPGTPAADMVDNVSEEEKKQR 361
Query: 355 SKKLIELNRINEKA--FAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVS 412
L E RI ++A ++ + G+V +L E +G T N VN + ++
Sbjct: 362 LHILQE--RITQQAMQYSRRMKGKVQRILVEGTSRKNVMELSGRTENNRVVNFEGAPSMI 419
Query: 413 GKILNVKI-----NSFEGEVAEGE 431
GK ++V+I NS G + E
Sbjct: 420 GKFVDVEIVDVYPNSLRGVLLRTE 443
>MIAB_SHEWM RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 474
Score = 187 bits (476), Expect = 1e-49
Identities = 134/444 (30%), Positives = 218/444 (49%), Gaps = 18/444 (4%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVR-EGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S M + +GY + + E ADV ++NTCS+ +K +
Sbjct: 4 KLHIKTWGCQMNEYDSSKMADLMDEYKGYTLTEEAEEADVLLLNTCSIREKAQEKVFHQL 63
Query: 61 GRARR---QNPEAIIAVVGCYA-QIAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR ++ +NP II V GC A Q + VD++ G + + +++ GK
Sbjct: 64 GRWKKLKDKNPNLIIGVGGCVASQEGKVIKDRAQCVDLIFGPQTLHRLPEMIDQIEAGGK 123
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
+ V K D E + AF+ I +GC+++C++C++PYTRG S+ V
Sbjct: 124 AVIDVSFPEIEKF--DRLPEPRADGPSAFVSIMEGCSKYCSFCVVPYTRGEEVSRPLDDV 181
Query: 177 LDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKV-TLVDLLEEIEKIDGIERVRIGSIDP 234
+ EI LAE G +E+ L G + +Y G D+++ T +LL + IDGI+R+R + P
Sbjct: 182 ILEIAQLAEQGVREVNLLGQNVNAYRGATHDDEICTFAELLRYVAAIDGIDRLRFTTSHP 241
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
FT+D++ +L HL +QSG D L +M R + A EY+ I+ LR D+
Sbjct: 242 IEFTQDIIDVYEDTPELVSFLHLPVQSGSDRILTQMKRGHMAIEYKSIIRRLRKARPDIQ 301
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
+++D I+GFPG ++ + + F YS R GT A ++ + V ++K ER
Sbjct: 302 VSSDFIIGFPGESKQDFEDTMKLIEDVQFDHSFSFIYSARPGTPASDLPDDVTLDEKKER 361
Query: 355 SKKLIELNRINEKA--FAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVS 412
I +RI ++A ++ + +G V +L E G T VN +AD
Sbjct: 362 --LAILQDRITQQAMRYSRQMLGTVQRILVEGPSVKNPMELRGRTETSRVVNFEADPKHI 419
Query: 413 GKILNVKI-----NSFEGEVAEGE 431
G ++V+I NS G GE
Sbjct: 420 GSFVDVEIVDVYTNSLRGNFIRGE 443
>MIAB_SHEHH RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 475
Score = 187 bits (476), Expect = 1e-49
Identities = 138/444 (31%), Positives = 222/444 (50%), Gaps = 18/444 (4%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVR-EGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S M + EGY + D E ADV ++NTCS+ +K +
Sbjct: 4 KLHIKTWGCQMNEYDSSKMADLLDEYEGYTLTDNAEEADVLLLNTCSIREKAQEKVFHQL 63
Query: 61 GR---ARRQNPEAIIAVVGCYA-QIAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR + + PE II V GC A Q + VD++ G + + +++ R GK
Sbjct: 64 GRWKTLKDKKPELIIGVGGCVASQEGKAIKERAQCVDLIFGPQTLHRLPEMIDQIR-AGK 122
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
+ V + + + F+ L E + AF+ I +GC+++C++C++PYTRG S+ +
Sbjct: 123 KAVIDVSFPEIEKFDRLP-EPRADGPSAFVSIMEGCSKYCSFCVVPYTRGEEVSRPLDDI 181
Query: 177 LDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKV-TLVDLLEEIEKIDGIERVRIGSIDP 234
+ EI LAE G +E+ L G + +Y G D+++ T +LL + IDGI+R+R + P
Sbjct: 182 ILEIAQLAEQGVREVNLLGQNVNAYRGATHDDEICTFAELLRYVAAIDGIDRLRFTTSHP 241
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
FT+D++ +L HL +QSG D L +M R + A EY+ I+ LR D+
Sbjct: 242 IEFTQDIIDVYEDTPELVSFLHLPVQSGSDLILTQMKRGHMAIEYKSIIRRLRKARPDIL 301
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
I++D I+GFPG ++ I + F YS R GT A ++ + V ++K ER
Sbjct: 302 ISSDFIIGFPGESKQDFADTMKLIEDIQFDHSFSFIYSARPGTPAADLPDDVSLDEKKER 361
Query: 355 SKKLIELNRINEKA--FAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVS 412
I +RI ++A ++ + +G V +L E G T N VN +
Sbjct: 362 --LAILQDRITQQAMRYSRQMVGTVQRILVEGPSVKNPMELRGRTENSRVVNFEGMHKHI 419
Query: 413 GKILNVKI-----NSFEGEVAEGE 431
GK ++V+I NS G GE
Sbjct: 420 GKFVDVEIVDVYTNSLRGVFIRGE 443
>MIAB_RHOFD RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 452
Score = 187 bits (476), Expect = 1e-49
Identities = 135/443 (30%), Positives = 205/443 (46%), Gaps = 13/443 (2%)
Query: 2 KVAFATLGCRVNTYESEAMTEKF-VREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
KV T GC++N Y+S+ M++ +GYE D E AD+ + NTCSV +K +
Sbjct: 4 KVFIKTYGCQMNEYDSDKMSDVLGAAQGYEPTDNVEEADLILFNTCSVREKAQEKVFSDL 63
Query: 61 GRARRQNPE-AIIAVVGCYA-QIAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQ 118
GR + + A+I V GC A Q + VDVV G + + + + + Q
Sbjct: 64 GRVKHLKKKGALIGVGGCVASQEGAAIIERAPYVDVVFGPQTLHRLPQLLAERERLNRSQ 123
Query: 119 VKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLD 178
V + K F+ L + AF+ I +GC+++C+YC++PYTRG S+ + VL
Sbjct: 124 VDISFPEIEK-FDHLPPARVEGAS-AFVSIMEGCSKYCSYCVVPYTRGEEVSRPFEDVLV 181
Query: 179 EIRSLAEHGFKEIILSGIHTASYGVDL--------DEKVTLVDLLEEIEKIDGIERVRIG 230
E+ LA+ G KEI L G + ++ + E LLE + I GIER+R
Sbjct: 182 EVAGLADQGVKEITLLGQNVNAWRSRMIGAASALSSEMADFATLLEYVSDIPGIERIRYV 241
Query: 231 SIDPTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKI 290
+ P FT ++ L KL H HL +Q G D L M R YTA EY+ V LR
Sbjct: 242 TSHPNEFTPSLIAAYDKLPKLVSHLHLPVQHGSDRILMAMKRGYTAMEYKSTVRKLRAIR 301
Query: 291 EDVSITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNK 350
D+++++D IVGFPG + + + F +SPR GT A N+ +
Sbjct: 302 PDMALSSDFIVGFPGETEDDFSKMMKLIDDVGFDNSFSFIFSPRPGTPAANLHDDTPHEV 361
Query: 351 KDERSKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCN 410
K R + L + + K +E +G V +L E + S G T VN
Sbjct: 362 KLRRLQHLQTVINDSIKRISESRLGTVQRILVEGASKRDSTELMGRTECNRVVNFCGQPR 421
Query: 411 VSGKILNVKINSFEGEVAEGEIV 433
+ G++++V I GE+V
Sbjct: 422 LIGQMVDVAITETRSFTLRGEVV 444
>MIAB_PSEU2 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 442
Score = 187 bits (476), Expect = 1e-49
Identities = 133/445 (29%), Positives = 219/445 (49%), Gaps = 20/445 (4%)
Query: 2 KVAFATLGCRVNTYESEAMTEKF-VREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S M + + EV E ADV ++NTCS+ + +
Sbjct: 4 KLYIETHGCQMNEYDSSRMVDLLGEHQALEVTARAEDADVILLNTCSIRERAQDRVYSQL 63
Query: 61 GRARR---QNPEAIIAVVGCYAQIAPTEVSGIEG----VDVVLGSRNKGEIVYYVNKARD 113
GR R NPE +IAV GC +A E + I VDVV G + + ++ AR
Sbjct: 64 GRWRELKLANPEMVIAVGGC---VASQEGAAIRDRAPYVDVVFGPQTLHRLPEMIDAARV 120
Query: 114 EGKQQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDP 173
QV V K F+ L + + A++ + +GC+++C +C++PYTRG S+
Sbjct: 121 TRLPQVDVSFPEIEK-FDHLPEPRVDGPS-AYVSVMEGCSKYCTFCVVPYTRGEEVSRPF 178
Query: 174 KKVLDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKVT-LVDLLEEIEKIDGIERVRIGS 231
VL E+ LAE+G +E+ L G + Y G D +V L DL+ + +DGI+R+R +
Sbjct: 179 DDVLSEVIHLAENGVREVTLLGQNVNGYRGTTHDGRVADLADLIRVVAAVDGIDRIRYTT 238
Query: 232 IDPTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIE 291
P F++ +++ + +L H HL +QSG D L M R +T EY+ + LR +
Sbjct: 239 SHPLEFSDSLIQAHAEVPELVKHLHLPVQSGSDRILAAMKRNHTTLEYKSRLRKLRAAVP 298
Query: 292 DVSITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKK 351
+SI++D IVGFPG ++ + + F YSPR GT A ++++ K
Sbjct: 299 GISISSDFIVGFPGETEKDFDNTMKLIEDVGFDFSFSFVYSPRPGTPAADLKDDTPEALK 358
Query: 352 DERSKKLIELNRINEKAF--AEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKAD- 408
ER L +R+N++ F + + +G + +L + + G G T N VN + D
Sbjct: 359 KERLAAL--QHRLNQQGFEISRQMVGSIQRILVTDYSKKDPGELQGRTENNRIVNFRCDN 416
Query: 409 CNVSGKILNVKINSFEGEVAEGEIV 433
+ G+ +V I+ + G ++
Sbjct: 417 PKLIGQFADVHIDDAQPHSLRGSLL 441
>MIAB_POLNA RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 447
Score = 187 bits (476), Expect = 1e-49
Identities = 131/438 (29%), Positives = 206/438 (47%), Gaps = 8/438 (1%)
Query: 2 KVAFATLGCRVNTYESEAMTEKF-VREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
KV T GC++N Y+S+ M++ EGYE + AD+ + NTCSV +K +
Sbjct: 4 KVFIKTFGCQMNEYDSDKMSDVLHAAEGYENTQNIDEADLILFNTCSVREKAQEKVFSDL 63
Query: 61 GRARRQNPEAI-IAVVGCYA-QIAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQ 118
GR + + + I V GC A Q + VDVV G + + + GK Q
Sbjct: 64 GRVKHLKAKGVLIGVGGCVASQEGEAIIQRAPYVDVVFGPQTLHRLPQLLASRARLGKPQ 123
Query: 119 VKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLD 178
V + K F+ L + AF+ I +GC+++C+YC++PYTRG S+ + VL
Sbjct: 124 VDISFPEIEK-FDHLPPARVEGAS-AFVSIMEGCSKYCSYCVVPYTRGEEVSRPFEDVLV 181
Query: 179 EIRSLAEHGFKEIILSGIHTASYGVDLDEKVTLVD---LLEEIEKIDGIERVRIGSIDPT 235
E+ LA+ G KE+ L G + +Y + + D LLE + +I GIER+R + P
Sbjct: 182 EVAGLADQGVKEVTLLGQNVNAYRGSMGGTAEIADFATLLEYVSEIPGIERIRYITSHPN 241
Query: 236 FFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSI 295
FT+ ++ L KL H HL +Q G D L M R YTA EY+ + LR D+++
Sbjct: 242 EFTQRLIDAYATLPKLVNHLHLPVQHGSDRILMAMKRGYTAMEYKSTIRKLRAIRPDLAM 301
Query: 296 TTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERS 355
++D IVGFPG + + + F +SPR GT A N+ + K +R
Sbjct: 302 SSDFIVGFPGETEDDFEKLMKLVNDVGYDTSFSFIFSPRPGTPAANLADDTPHEVKLKRL 361
Query: 356 KKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSGKI 415
+ L + +A + +G V +L E + G T V K + G++
Sbjct: 362 QHLQATLDDSVRAISASRLGTVQRILVEGTARKDASELMGRTECNRVVILKGQPRLIGQM 421
Query: 416 LNVKINSFEGEVAEGEIV 433
++V+I GE++
Sbjct: 422 VDVRITEASQRSLRGEVL 439
>MIAB_NOCFA RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 503
Score = 187 bits (476), Expect = 1e-49
Identities = 128/395 (32%), Positives = 184/395 (46%), Gaps = 16/395 (4%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIGR---A 63
T GC++N ++SE ++ GY AD+ V NTC+V D K +G
Sbjct: 11 TFGCQMNVHDSERLSGLLEDAGYVKAAPGATADLVVFNTCAVRENADNKLYGTLGHLAPV 70
Query: 64 RRQNPEAIIAVVGCYAQI-APTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVKVG 122
+ P IAV GC AQ T V VDVV G+ N G + + +AR + QV++
Sbjct: 71 KAGRPGMQIAVGGCLAQKDRDTVVRKAPWVDVVFGTHNIGSLPVLLERARHNEQAQVEIL 130
Query: 123 AVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDEIRS 182
L+ F + + ++ I GCN C +C++P RG + P VL E+++
Sbjct: 131 ESLE--AFPSTLPAKRESAYAGWVSISVGCNNTCTFCIVPALRGKEVDRRPGDVLAEVQA 188
Query: 183 LAEHGFKEIILSGIHTASYGV---DLDE---KVTLVDLLEEIEKIDGIERVRIGSIDPTF 236
L + G E+ L G + SYG D DE + LL I+G+ERVR S P
Sbjct: 189 LVDQGVLEVTLLGQNVNSYGASFADPDEPRDRGAFAKLLRACGGIEGLERVRFTSPHPAE 248
Query: 237 FTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSIT 296
FT+DV+ + +CP H+ LQSG D LK M R Y Y I+ +R + +IT
Sbjct: 249 FTDDVIEAMAQTPNICPQLHMPLQSGSDRVLKAMRRSYRKARYLGIIEKVRAAMPHAAIT 308
Query: 297 TDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSK 356
TD+IVGFPG +++ + F+YS R GT A M +QV ER
Sbjct: 309 TDIIVGFPGETEEDFQETLDVVRQARFTSAFTFQYSKRPGTPAAEMADQVPKQVVQERYD 368
Query: 357 KLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSG 391
+LI L A + IG +++L E GSG
Sbjct: 369 RLIALQEEISLAANRELIGTEVELLVAE----GSG 399
>MIAB_WOLTR RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 442
Score = 187 bits (475), Expect = 1e-49
Identities = 123/385 (31%), Positives = 192/385 (49%), Gaps = 7/385 (1%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIGR--AR 64
T GC++N Y+S M G+ VV+ E AD+ ++NTC + +K +G+ +
Sbjct: 8 TYGCQMNVYDSILMENIIKPLGFNVVNDVEKADLVILNTCHIREKAAEKLYSELGKIHSS 67
Query: 65 RQNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVKVGA 123
R+N E I GC AQ E+ VD+V+G ++ + + KA K V
Sbjct: 68 RKNKEITIVAAGCVAQAEGEEIFRRAPFVDIVVGPQSITTLPELIVKA-SRSKGHVINTD 126
Query: 124 VLKNKVFEDLKIEEYNNK--TRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDEIR 181
K F+ L E Y N + AFL IQ+GC++FC +C++PYTRG+ S+ ++ E
Sbjct: 127 FPKVMKFDRLSDECYGNSQGSSAFLSIQEGCDKFCTFCVVPYTRGAEYSRPVNEIFREAL 186
Query: 182 SLAEHGFKEIILSGIHTASYGVDLDEKV-TLVDLLEEIEKIDGIERVRIGSIDPTFFTED 240
L +G KEI L G + +Y + + +V L L+ I KI+ +ER+R + P E
Sbjct: 187 KLVANGAKEINLLGQNVNAYHGEYEGEVWDLGKLISHIAKIEKLERIRYTTSHPRDMHES 246
Query: 241 VVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITTDVI 300
+ KL P HL +QSG + L+ MNR+YT +EY +I+ R + ++D I
Sbjct: 247 LYLAHAEAPKLMPFIHLPVQSGSNKILRAMNRKYTTEEYLEIIERFRKLKPKIEFSSDFI 306
Query: 301 VGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSKKLIE 360
VGFPG ++R+ ++ + FKYS R GT ++QV K ER L +
Sbjct: 307 VGFPGETEKDFEGTIKLVERVRYAQAYSFKYSTRPGTPGAERKDQVPEKVKTERLLHLQK 366
Query: 361 LNRINEKAFAEKYIGEVIDVLFEEE 385
L + F + +G+ I VLF +
Sbjct: 367 LINKQQLEFNQSMVGKTIPVLFSNK 391
>MIAB_MARMS RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 451
Score = 187 bits (474), Expect = 2e-49
Identities = 136/449 (30%), Positives = 220/449 (48%), Gaps = 26/449 (5%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVREGYEV--VDFNEMADVYVVNTCSVTNMGDKKSRQI 59
K+ T GC++N Y+S M + + E +E+ D E ADV ++NTCS+ K
Sbjct: 4 KLFIQTHGCQMNEYDSSRMAD-LLGESHEMELTDNAEEADVLLLNTCSIREKAQDKVYHQ 62
Query: 60 IGR---ARRQNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEG 115
+GR +++NP +I V GC A + + VD++ G + ++ VN A G
Sbjct: 63 LGRWKKLKQKNPNLVIGVGGCVASQEGDAIRKRAKHVDMIFGPQTLHKLPEMVNAA---G 119
Query: 116 KQQVKVGAVLKNKV--FEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDP 173
K + + V ++ F+ L AF+ I +GC+++C +C++PYTRG S+
Sbjct: 120 KH-IPITDVTFPEIEKFDHLPAPRVEG-AEAFVSIMEGCSKYCTFCVVPYTRGEEVSRPF 177
Query: 174 KKVLDEIRSLAEHGFKEIILSGIHTASYGVDLDE--KVTLVDLLEEIEKIDGIERVRIGS 231
+L E+ LAE G +EI L G + +Y D E + L D++ + +IDG+ER+R +
Sbjct: 178 DSILKEVVQLAEQGVREIHLLGQNVNAYRGDTAEGDEADLADIIHAVAQIDGVERIRFTT 237
Query: 232 IDPTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIE 291
P F++ ++ KL H HL +QSG D L M R + + Y D +N +++
Sbjct: 238 SHPVEFSDSLIEAFRNEPKLVSHLHLPVQSGADNILSAMKRGHDRQYYIDKINRIKEARP 297
Query: 292 DVSITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKK 351
+S+++D I+GFPG ++ I + F YS R GT A N+E+ + K
Sbjct: 298 GISLSSDFIIGFPGETDDDFVDTMNLIQEIGFDHSFSFVYSQRPGTPASNLEDDTPEDVK 357
Query: 352 DERSKKLIELNRINEKAF--AEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKA-D 408
ER I RI+++A+ + +GEV +L G G T N VN +A D
Sbjct: 358 KER--LAILQRRISQQAYDISLSMVGEVQRILISGYSPRDPGQLQGRTENNRIVNFRAFD 415
Query: 409 CNVSGKILNVKI-----NSFEGEVAEGEI 432
+ GK +V I NS GE+ E+
Sbjct: 416 PQLIGKFADVVITDAYPNSLLGELVGSEL 444
>MIAB_HYPNA RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 449
Score = 187 bits (474), Expect = 2e-49
Identities = 132/436 (30%), Positives = 208/436 (47%), Gaps = 17/436 (3%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIGRARRQ 66
T GC++N Y+SE + + GY V+ E AD+ VVNTC + +K +G+ +R
Sbjct: 8 TYGCQMNVYDSERIRDVLRPLGYAPVETPESADLVVVNTCHIREKATEKVYSELGQLKRM 67
Query: 67 NPEA----IIAVVGCYAQIAPTEVSGIE-GVDVVLGSRNKGEIVYYVNKARDEGKQQVKV 121
+ IAV GC AQ E+ + VD+VLG + ++ + +A +++
Sbjct: 68 KEASGGRMTIAVAGCVAQAEGEELIRRQPAVDLVLGPQAYHKLPEMIARASRAIGDRLET 127
Query: 122 GAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDEIR 181
K F+ L + AF+ +Q+GC++FC +C++PYTRG+ S+ ++ E R
Sbjct: 128 EFDTVEK-FDALPKTREADGPAAFVSVQEGCDKFCTFCVVPYTRGAEMSRRVDDIVFETR 186
Query: 182 SLAEHGFKEIILSG-----IHTASYGVDLDEKVTLVDLLEEIEKIDGIERVRIGSIDPTF 236
SLA G +EI L G H + ++ E TL L + KI GIER+R + P
Sbjct: 187 SLASQGVREITLLGQNVNAFHGPAPVLEGGEDWTLGQLCRHLSKIGGIERIRYTTSHPRD 246
Query: 237 FTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSIT 296
+D++ + P HL +QSG D LK MNR +TA Y DI+ +R D++I
Sbjct: 247 MDDDLIAAHGDTPAMMPFLHLPVQSGSDRILKAMNRGHTADHYRDIITRVRAARPDIAIA 306
Query: 297 TDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSK 356
+D IVGFPG ++ I + + FKYS R GT A M + + KD
Sbjct: 307 SDFIVGFPGESDADFEATMQLVRDIGYAIAYSFKYSSRPGTPAAEMHGHLSESVKDXXXX 366
Query: 357 KLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSGKIL 416
F IG+ + VL + +G G + V+ + +++GKI+
Sbjct: 367 XXXXXXXXXXTEFNASQIGKTLPVLVTGKGR-NAGQMHGRSPYLQAVHFEGPDDLNGKIV 425
Query: 417 NVKI-----NSFEGEV 427
+VK+ NS GE+
Sbjct: 426 DVKVIGASLNSLTGEL 441
>MIAB_ERWCT RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 474
Score = 187 bits (474), Expect = 2e-49
Identities = 139/445 (31%), Positives = 216/445 (48%), Gaps = 20/445 (4%)
Query: 2 KVAFATLGCRVNTYESEAMTEKF-VREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S M + GYE+ + E ADV ++NTCS+ +K +
Sbjct: 4 KLHIKTWGCQMNEYDSSKMADLLGSTHGYELTEIAEEADVLLLNTCSIREKAQEKVFHQL 63
Query: 61 GR---ARRQNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR + NP II V GC A + VDV+ G + + +N +
Sbjct: 64 GRWKALKDLNPNLIIGVGGCVASQEGAHIRERAHYVDVIFGPQTLHRLPEMINHVQGTRS 123
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
V + K F+ L + T AF+ I +GCN++C +C++PYTRG S+ V
Sbjct: 124 PIVDISFPEIEK-FDRLPEPRADGPT-AFVSIMEGCNKYCTFCVVPYTRGEEVSRPCDDV 181
Query: 177 LDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKV-TLVDLLEEIEKIDGIERVRIGSIDP 234
L EI LA G +E+ L G + +Y G D ++ + +LL + IDGI+RVR + P
Sbjct: 182 LFEIAQLAAQGVREVNLLGQNVNAYRGETYDGEICSFAELLRLVAAIDGIDRVRFTTSHP 241
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
FT+D++ +L HL +QSG D L M RR+TA EY+ I+ L + +
Sbjct: 242 IEFTDDIISVYEDTPELVSFLHLPVQSGSDRVLTMMKRRHTALEYKAIIRKLHNARPGIL 301
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
I++D I+GFPG + + ++ F YS R GT A +M VD ++E+
Sbjct: 302 ISSDFIIGFPGETQADFEQTMKLIADVNFDMSYSFVYSARPGTPAADM---VDDVLEEEK 358
Query: 355 SKKLIEL-NRINEKA--FAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNV 411
++L L +RI ++A F+ +G V +L E +G T N VN + ++
Sbjct: 359 KQRLYLLQDRITKQAMRFSRLMLGTVQRILVEGTSRKSVMELSGRTENNRVVNFEGTPDM 418
Query: 412 SGKILNVKI-----NSFEGEVAEGE 431
GK ++V+I NS G V E
Sbjct: 419 IGKFVDVEIVDVYTNSLRGIVVRTE 443
>MIAB_ACIAC RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 449
Score = 187 bits (474), Expect = 2e-49
Identities = 131/438 (29%), Positives = 204/438 (46%), Gaps = 8/438 (1%)
Query: 2 KVAFATLGCRVNTYESEAMTEKF-VREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
KV T GC++N Y+S+ M + +GYE E AD+ + NTCSV +K +
Sbjct: 4 KVFVKTFGCQMNEYDSDKMVDVLNAAQGYEPTQDVEEADLILFNTCSVREKAQEKVFSDL 63
Query: 61 GRARRQNPEAI-IAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQ 118
GR + + I V GC A E+ VDVV G + + + + + Q
Sbjct: 64 GRIKHLKARGVKIGVGGCVASQEGDEIIKRAPYVDVVFGPQTLHRLPELLAQREALARPQ 123
Query: 119 VKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLD 178
V + K F+ L + AF+ I +GC+++C+YC++PYTRG S+ VL
Sbjct: 124 VDISFPEIEK-FDHLPPARVEGAS-AFVSIMEGCSKYCSYCVVPYTRGEEVSRPFDDVLV 181
Query: 179 EIRSLAEHGFKEIILSGIHTASYGVDLDEKVTLVD---LLEEIEKIDGIERVRIGSIDPT 235
E+ LA+ G KE+ L G + +Y + + D LLE + I GIER+R + P
Sbjct: 182 EVAGLADQGVKEVTLLGQNVNAYLGKMGGTAEVADFALLLEYVADITGIERIRFTTSHPN 241
Query: 236 FFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSI 295
FT+ ++ + KL H HL +Q G D L M R YTA EY+ + LR D+++
Sbjct: 242 EFTQRLIDAYARIPKLVSHLHLPVQHGSDRILMAMKRGYTAMEYKSTIRKLRAIRPDLAM 301
Query: 296 TTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERS 355
++D IVGFPG + I + F +SPR GT A ++ + K R
Sbjct: 302 SSDFIVGFPGETEDDFGKMMKLIDDIHFDNSFSFIFSPRPGTPAASLHDDTPHEVKLRRL 361
Query: 356 KKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSGKI 415
++L + N K+ +E +G V +L E + G T VN + G++
Sbjct: 362 QELQAVINANIKSISESRVGTVQRILVEGASKRDGSELMGRTECNRVVNFAGHPRLVGQM 421
Query: 416 LNVKINSFEGEVAEGEIV 433
++V I + GE+V
Sbjct: 422 VDVTITEAKAYTLRGEVV 439
>MIAB_WOLSU RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 433
Score = 186 bits (473), Expect = 3e-49
Identities = 133/425 (31%), Positives = 205/425 (48%), Gaps = 14/425 (3%)
Query: 2 KVAFATLGCRVNTYESEAM-TEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ TLGC +N +SE M E +EGY + D + AD+ ++NTCSV ++K I
Sbjct: 4 KLFIQTLGCAMNVRDSEHMIAELEAKEGYTLTDDPKEADLILINTCSVREKPERKLFSEI 63
Query: 61 GR-ARRQNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQ 118
G+ ++ + EA I V GC A +E+ V VLG+RN +I +++ E +
Sbjct: 64 GQFSKEKKEEAKIGVCGCTASHLGSEILKKAPSVSFVLGARNVSKISRVIHQ---EKAVE 120
Query: 119 VKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLD 178
V + VF +Y +A + I GC++ CAYC++P+TRG S +L
Sbjct: 121 VATDYDDSSYVFATGSRNDY----KAMVNISIGCDKKCAYCIVPHTRGQEISVPSDLILG 176
Query: 179 EIRSLAEHGFKEIILSGIHTASYGVDLDE---KVTLVDLLEEIEKIDGIERVRIGSIDPT 235
E R LA G KEI+L G + YG K++ +LL E+ +++GIER+R S P
Sbjct: 177 EARRLASAGVKEILLLGQNVNHYGRRFSSAHPKISFTELLRELSQVEGIERLRFTSPHPL 236
Query: 236 FFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSI 295
++ + + K+C H+ LQSG L M R Y+ + + + V L+ + +I
Sbjct: 237 HMDDEFLEEFASNPKICKSIHMPLQSGSTRILSLMRRGYSQEWFINRVERLKALAPETTI 296
Query: 296 TTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERS 355
TD+IVGFPG L+R+ + F YSPR T A + EN VD ER
Sbjct: 297 GTDIIVGFPGESEEDFLGTMEVLERVRFETLYSFVYSPRPHTEAASWENLVDEEVASERL 356
Query: 356 KKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSGKI 415
+L ++ + +K +G + VL+E + G G T V K G+I
Sbjct: 357 HRLQARHKEILEELNQKEVGAIHSVLWEHQ-RRDEGWCEGRTDTGKMVRMKGGEEFLGRI 415
Query: 416 LNVKI 420
V+I
Sbjct: 416 TPVRI 420
>MIAB_VIBVU RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 474
Score = 186 bits (473), Expect = 3e-49
Identities = 130/439 (29%), Positives = 205/439 (46%), Gaps = 9/439 (2%)
Query: 2 KVAFATLGCRVNTYESEAMTEKF-VREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S M + GYE+ + E ADV ++NTCS+ +K +
Sbjct: 4 KLLIKTWGCQMNEYDSSKMADLLNAANGYELTEEPEEADVLLLNTCSIREKAQEKVFHQL 63
Query: 61 GR---ARRQNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR + + P +I V GC A + VDV+ G + + + +++ E
Sbjct: 64 GRWKTLKDKKPGVVIGVGGCVATQEGDHIRERAPFVDVIFGPQTLHRLPEMIKQSQSEDA 123
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
+ + K F+ L T AF+ I +GC+++C YC++PYTRG S+ V
Sbjct: 124 PVMDISFPEIEK-FDSLPEPRAEGAT-AFVSIMEGCSKYCTYCVVPYTRGEEVSRPMDDV 181
Query: 177 LDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKV-TLVDLLEEIEKIDGIERVRIGSIDP 234
L EI LAE G +E+ L G + +Y G D ++ + +LL + IDGI+R+R + P
Sbjct: 182 LYEIAQLAEQGVREVNLLGQNVNAYRGPTHDGEICSFAELLRLVASIDGIDRIRFTTSHP 241
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
FT+D++ +L HL +QSG D L M R +T EY+ I+ LR D+
Sbjct: 242 LEFTDDIIAVYEDTPELVSFLHLPVQSGSDRILTMMKRPHTGIEYKSIIRKLRKARPDIQ 301
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
I++D IVGFPG +K + + F +SPR GT A + + K ER
Sbjct: 302 ISSDFIVGFPGESDKDFQDTMKLIKDVDFDMSFSFIFSPRPGTPAADYPCDLSEETKKER 361
Query: 355 SKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSGK 414
+L + ++ +G VL E + T N VN + ++ G+
Sbjct: 362 LYELQQQINAQAMRYSRLMLGTEQRVLVEGPSKKNLMELRARTENNRVVNFEGSADLIGQ 421
Query: 415 ILNVKINSFEGEVAEGEIV 433
++VKI GEIV
Sbjct: 422 FVDVKITDVFANSLRGEIV 440
>MIAB_STRMK RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 472
Score = 186 bits (473), Expect = 3e-49
Identities = 129/426 (30%), Positives = 203/426 (47%), Gaps = 9/426 (2%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVR-EGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S M + +G E+ D E ADV +VNTCS+ +K +
Sbjct: 34 KLYIKTHGCQMNEYDSAKMADVLAASDGLELTDTPEDADVILVNTCSIREKAQEKVFSQL 93
Query: 61 G---RARRQNPEAIIAVVGCYA-QIAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
G + + E II V GC A Q + VD+V G + + + R++ +
Sbjct: 94 GVWKGLKNKGREVIIGVGGCVASQEGEAIIKRAPFVDLVFGPQTLHRLPELIRARREQKR 153
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
QV + K D E AF+ I +GC+++C++C++PYTRG+ S+ + V
Sbjct: 154 PQVDISFPEIEKF--DRLPEPRAEGASAFVSIMEGCSKYCSFCVVPYTRGTEVSRPFEDV 211
Query: 177 LDEIRSLAEHGFKEIILSGIHTASYGVDLD--EKVTLVDLLEEIEKIDGIERVRIGSIDP 234
+ E+ LA G +EI L G + +Y E L L+ I +IDG+ R+R + P
Sbjct: 212 VVEVAQLAAQGVREINLLGQNVNAYRGPYGDGEFADLGLLIRTIAEIDGVGRIRFTTSHP 271
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
F++ ++ + +L HL +Q+G D L M R YTA E++ + LR D+S
Sbjct: 272 LEFSDSLIDAFRDVPQLANFLHLPVQAGSDRVLSAMKRGYTALEFKSKIRKLRAVRPDIS 331
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
I++D IVGFPG ++ I + F YS R GT A ++E+ + +K R
Sbjct: 332 ISSDFIVGFPGETDADFEKTMKLIEDIGFDHSFSFIYSRRPGTPAADLEDTISDAEKHAR 391
Query: 355 SKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSGK 414
+L E + +EK +G V VL E TG T N VN A + G+
Sbjct: 392 LSRLQERINAHAAGISEKMVGTVQTVLVEGPSRKNPNELTGKTENMRSVNFPAPARLIGQ 451
Query: 415 ILNVKI 420
++V I
Sbjct: 452 FVDVVI 457
>MIAB_SHESA RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 474
Score = 186 bits (473), Expect = 3e-49
Identities = 133/444 (29%), Positives = 216/444 (48%), Gaps = 18/444 (4%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVR-EGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S M + +GY + + E AD+ ++NTCS+ +K +
Sbjct: 4 KLHIKTWGCQMNEYDSSKMADLLGEYQGYTLTEEAEEADILLLNTCSIREKAQEKVFHQL 63
Query: 61 GR---ARRQNPEAIIAVVGCYAQIAPTEVSG-IEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR + +NP+ II V GC A + + VD++ G + + +++ R K
Sbjct: 64 GRWKTLKDKNPDLIIGVGGCVASQEGKAIKDRAQCVDIIFGPQTLHRLPDMIDQVRRGEK 123
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
+ V K F+ L T AF+ I +GC+++C++C++PYTRG S+ +
Sbjct: 124 AVIDVSFPEIEK-FDRLPEPRAEGPT-AFVSIMEGCSKYCSFCVVPYTRGEEVSRPSDDI 181
Query: 177 LDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKV-TLVDLLEEIEKIDGIERVRIGSIDP 234
+ EI LAE G +E+ L G + +Y G D + T +LL + IDGI+R+R + P
Sbjct: 182 ILEIAQLAEQGVREVNLLGQNVNAYRGATHDGGICTFAELLRYVAAIDGIDRIRFTTSHP 241
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
FT+D++ +L HL +QSG D L M R + A EY+ I+ LR D+
Sbjct: 242 IEFTQDIIDVYEDTPELVSFLHLPVQSGSDRILTAMKRGHMAIEYKSIIRRLRKARPDIQ 301
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
I++D I+GFPG ++ +A + F YS R GT A ++ + VD +K +R
Sbjct: 302 ISSDFIIGFPGETKEDFADTMKLIEDVAFDHSFSFIYSARPGTPAADLPDDVDMEEKKQR 361
Query: 355 SKKLIELNRINEKA--FAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVS 412
I +RI ++A ++ +G V +L E G T N VN +
Sbjct: 362 --LAILQDRITQQAMRYSRHMMGTVQRILVEGPSVKNPMELRGRTENNRVVNFEGQPKHI 419
Query: 413 GKILNVKI-----NSFEGEVAEGE 431
G ++V+I NS G+ GE
Sbjct: 420 GSFVDVEIVDVYTNSLRGKFIRGE 443
>MIAB_PSE14 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 442
Score = 186 bits (473), Expect = 3e-49
Identities = 134/447 (29%), Positives = 220/447 (49%), Gaps = 24/447 (5%)
Query: 2 KVAFATLGCRVNTYESEAMTEKF-VREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S M + + EV E ADV ++NTCS+ + +
Sbjct: 4 KLYIETHGCQMNEYDSSRMVDLLGEHQALEVTARAEDADVILLNTCSIRERAQDRVYSQL 63
Query: 61 GRARR---QNPEAIIAVVGCYAQIAPTEVSGIEG----VDVVLGSRNKGEIVYYVNKARD 113
GR R NPE +IAV GC +A E + I VDVV G + + ++ AR
Sbjct: 64 GRWRELKLANPEMVIAVGGC---VASQEGAAIRDRAPYVDVVFGPQTLHRLPEMIDAARI 120
Query: 114 EGKQQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDP 173
QV V K F+ L + + A++ + +GC+++C +C++PYTRG S+
Sbjct: 121 TRLPQVDVSFPEIEK-FDHLPEPRVDGPS-AYVSVMEGCSKYCTFCVVPYTRGEEVSRPF 178
Query: 174 KKVLDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKVT-LVDLLEEIEKIDGIERVRIGS 231
VL E+ LAE+G +E+ L G + Y G D +V L DL+ + +DGI+R+R +
Sbjct: 179 DDVLSEVIHLAENGVREVTLLGQNVNGYRGTTHDGRVADLADLIRVVAAVDGIDRIRYTT 238
Query: 232 IDPTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIE 291
P F++ +++ + +L H HL +QSG D L M R +T EY+ + LR +
Sbjct: 239 SHPLEFSDSLIQAHAEVPELVKHLHLPVQSGSDRILAAMKRNHTTLEYKSRLRKLRAAVP 298
Query: 292 DVSITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKK 351
+SI++D IVGFPG ++ + + F YSPR GT A ++++ K
Sbjct: 299 GISISSDFIVGFPGETEKDFDNTMKLIEDVGFDFSFSFVYSPRPGTPAADLKDDTPEALK 358
Query: 352 DERSKKLIELNRINEKAF--AEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADC 409
ER L +R+N++ F + + +G + +L + + G G T N VN + C
Sbjct: 359 KERLAAL--QHRLNQQGFEISRQMVGSIQRILVTDYSKKDPGELQGRTENNRIVNFR--C 414
Query: 410 N---VSGKILNVKINSFEGEVAEGEIV 433
N + G+ +V I+ + G ++
Sbjct: 415 NNPKLIGQFADVHIDDAQPHSLRGSLL 441
>MIAB_PROMH RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 476
Score = 186 bits (473), Expect = 3e-49
Identities = 131/444 (29%), Positives = 213/444 (47%), Gaps = 18/444 (4%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVR-EGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S M + GY++ D E AD+ ++NTCS+ +K +
Sbjct: 6 KLYIKTWGCQMNEYDSSKMADLLESTHGYQLTDVAEDADILLLNTCSIREKAQEKVFHQL 65
Query: 61 GRARR---QNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR + P+ II V GC A + + VD++ G + + +N+ +
Sbjct: 66 GRWKNFKDNKPDIIIGVGGCVASQEGEYIRQRAQCVDIIFGPQTLHRLPEMINQVKGTKS 125
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
V + K D E AF+ I +GCN++C++C++PYTRG S+ +
Sbjct: 126 PVVDISFPEIEKF--DRLPEPRAEGPSAFVSIMEGCNKYCSFCVVPYTRGEEVSRPCDDI 183
Query: 177 LDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKV-TLVDLLEEIEKIDGIERVRIGSIDP 234
L EI LA G +E+ L G + +Y G D ++ + +LL + IDGI+R+R + P
Sbjct: 184 LFEIAQLAAQGVREVNLLGQNVNAYRGETFDGQICSFAELLRLVAAIDGIDRIRFTTSHP 243
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
FT+D++ +L HL +QSG D L M R +TA EY+ I+ LR D+
Sbjct: 244 IEFTDDIIAVYEDTPELVSFLHLPVQSGSDRILTLMKRNHTALEYKSIIRKLRKARPDIL 303
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
I++D I+GFPG + + + F YS R GT A ++ + V +K +R
Sbjct: 304 ISSDFIIGFPGETQDDFEKTMKLIADVNFDMSFSFIYSARPGTPAADLPDDVSEEEKKQR 363
Query: 355 SKKLIELNRINEKA--FAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVS 412
L + RIN++A ++ + V +L E +G T N VN + ++
Sbjct: 364 LYLLQQ--RINQQAMNYSRAMLNSVQRILVEGPSRKNVMELSGRTENNRVVNFEGTPDMI 421
Query: 413 GKILNVKI-----NSFEGEVAEGE 431
GK ++V+I NS G+V E
Sbjct: 422 GKFVDVEIVDVYANSLRGKVVRTE 445
>MIAB_NEOSM RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 471
Score = 186 bits (473), Expect = 3e-49
Identities = 130/432 (30%), Positives = 215/432 (49%), Gaps = 21/432 (4%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVRE-GYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K T GC++N Y+SE M EK V G+ + + E AD+ ++NTC++ +K +
Sbjct: 30 KFHIKTYGCQMNVYDSE-MIEKIVSGLGFTLSERAEDADLIILNTCNIREKAAEKLYSEL 88
Query: 61 GRAR----RQNPEAIIAVVGCYAQIAPTEVSG-IEGVDVVLGSRNKGEIVYYVNKARDEG 115
G+ R ++ +I V GC AQ E+ E VDVV+G ++ + + K +
Sbjct: 89 GQIRLLQKKKQERILIVVAGCVAQAEGEEIMRRAENVDVVVGPQSIHSLPELIAKVNRQS 148
Query: 116 KQQVKVGAVLKNKVFEDLKIEEYNNK----TRAFLKIQDGCNRFCAYCLIPYTRGSVCSK 171
+ +K+ K D EE + + AFL IQ+GC++FCA+C++PYTRG+ S+
Sbjct: 149 GKAIKMEFDPIEKF--DYLAEETRKRRVPQSSAFLSIQEGCDKFCAFCVVPYTRGAEYSR 206
Query: 172 DPKKVLDEIRSLAEHGFKEIILSGIHTASYGVDLD---EKVTLVDLLEEIEKIDGIERVR 228
++V E SL G KEI L G + Y LD + + L L+ + KI ++R+R
Sbjct: 207 STEEVYREALSLTTKGVKEITLLGQNVNGYHGTLDSGNKVLNLGQLISRLGKIPSLKRIR 266
Query: 229 IGSIDPTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRD 288
+ P +++ KL P HL +QSG D LK+MNR+YT +Y I+N ++
Sbjct: 267 YTTSHPVDMHKELYDAHANESKLMPFVHLPVQSGSDKILKQMNRKYTTADYLKIINEFQN 326
Query: 289 KIEDVSITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDG 348
D++ ++D IVGFPG ++++ ++ + FKYSPR GT Q+
Sbjct: 327 ARSDIAFSSDFIVGFPGESDDDFQQTLALIEQVNYAQCYSFKYSPRPGTPGATYP-QISE 385
Query: 349 NKKDERSKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKAD 408
K+ R F +K IG+ + VLF+++ +G T Y++ D
Sbjct: 386 ETKNTRXXXXXXXXXXXXXXFNKKMIGKTVTVLFDKK---HPDKISGRT-EYMQQVFSDD 441
Query: 409 CNVSGKILNVKI 420
N+ KI+ +++
Sbjct: 442 SNLLDKIVTMRV 453
>MIAB_GEOMG RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 441
Score = 186 bits (473), Expect = 3e-49
Identities = 130/432 (30%), Positives = 212/432 (49%), Gaps = 14/432 (3%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKK--SRQIIGRAR 64
T GC++N +SE + EGY + AD+ +VNTCSV + K S
Sbjct: 11 TFGCQMNVSDSEKVASLLRGEGYSQTPDSSEADLIIVNTCSVRAKAEHKVYSYXXXXXXX 70
Query: 65 RQNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVKVGA 123
C AQ + + +D+V G+ N + V +A ++G+++ V
Sbjct: 71 XXXXXXXXXXXXCVAQQEGERLLKRVPWLDLVFGTHNLHLLPEMV-RAAEQGERRAAVDF 129
Query: 124 VLKNKVFEDL--KIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDEIR 181
+ N+ DL + +E TR F+ + GC+ FC+YC++PY RG S+ +++ EIR
Sbjct: 130 I-DNEARLDLFPQADEGGGVTR-FVTVMQGCDNFCSYCIVPYVRGREISRRSVEIIGEIR 187
Query: 182 SLAEHGFKEIILSGIHTASYGVDLDEKVTLVDLLEEIEKIDGIERVRIGSIDPTFFTEDV 241
S G +E+ L G + SYG+ +++ LL EI IDG+ER+R + P + ++
Sbjct: 188 SAVAGGVREVTLLGQNVNSYGLKTPGELSFAGLLREISAIDGLERIRFTTSHPKDISPEL 247
Query: 242 VRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITTDVIV 301
+ L KLC H HL Q+G D+ L RMNR YT +EY + V LR ++ IT D+IV
Sbjct: 248 IACFAELPKLCGHIHLPAQAGSDSILARMNRGYTRQEYLEKVAALRAARPEILITGDIIV 307
Query: 302 GFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSKKLIEL 361
GFPG ++ + + F YSPR T A ++ +++ + ER +++ L
Sbjct: 308 GFPGETEADFLQTLSLMEEVRYTDIFSFAYSPRPETAAASLGDRIMRKETTERLERVQGL 367
Query: 362 NRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSGKILNVKI- 420
R ++G VL E + G +Y G T + VN + +++G +++V+I
Sbjct: 368 QRDMTIERHAGFVGTCQAVLVEGMSKRGDQLY-GRTDGNLIVNFAGNPSLAGSLVDVRIT 426
Query: 421 ----NSFEGEVA 428
NS GE+A
Sbjct: 427 RGYPNSLLGELA 438
>MIAB_VIBVY RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 474
Score = 186 bits (472), Expect = 3e-49
Identities = 129/439 (29%), Positives = 203/439 (46%), Gaps = 9/439 (2%)
Query: 2 KVAFATLGCRVNTYESEAMTEKF-VREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S M + GYE+ + E ADV ++NTCS+ +K +
Sbjct: 4 KLLIKTWGCQMNEYDSSKMADLLNAANGYELTEEPEEADVLLLNTCSIREKAQEKVFHQL 63
Query: 61 GR---ARRQNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR + + P +I V GC A + VDV+ G + + + +++ E
Sbjct: 64 GRWKTLKDKKPGVVIGVGGCVATQEGDHIRERAPFVDVIFGPQTLHRLPEMIKQSQSEDA 123
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
+ + K F+ L T AF+ I +GC+++C YC++PYTRG S+ V
Sbjct: 124 PVMDISFPEIEK-FDSLPEPRAEGAT-AFVSIMEGCSKYCTYCVVPYTRGEEVSRPMDDV 181
Query: 177 LDEIRSLAEHGFKEIILSGIHTASYGVDLD--EKVTLVDLLEEIEKIDGIERVRIGSIDP 234
L EI LAE G +E+ L G + +Y + E + +LL + IDGI+R+R + P
Sbjct: 182 LYEIAQLAEQGVREVNLLGQNVNAYRGPMHDGEICSFAELLRLVASIDGIDRIRFTTSHP 241
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
FT+D++ +L HL +QSG D L M R +T EY+ I+ LR D+
Sbjct: 242 LEFTDDIIAVYEDTPELVSFLHLPVQSGSDRILTMMKRPHTGIEYKSIIRKLRKARPDIQ 301
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
I++D IVGFPG +K + + F +SPR GT A + + K ER
Sbjct: 302 ISSDFIVGFPGETDKDFQDTMKLIKDVDFDMSFSFIFSPRPGTPAADYPCDLSEETKKER 361
Query: 355 SKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSGK 414
+L + ++ +G VL E + T N VN + ++ G+
Sbjct: 362 LYELQQQINAQAMRYSRLMLGTEQRVLVEGPSKKNLMELRARTENNRVVNFEGSADLIGQ 421
Query: 415 ILNVKINSFEGEVAEGEIV 433
++VKI GEIV
Sbjct: 422 FVDVKITDVFANSLRGEIV 440
>MIAB_STRM5 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 472
Score = 186 bits (472), Expect = 3e-49
Identities = 128/426 (30%), Positives = 204/426 (47%), Gaps = 9/426 (2%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVR-EGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S M + +G E+ D + ADV +VNTCS+ +K +
Sbjct: 34 KLYIKTHGCQMNEYDSAKMADVLAASDGLELTDSPDDADVILVNTCSIREKAQEKVFSQL 93
Query: 61 G---RARRQNPEAIIAVVGCYA-QIAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
G + + E II V GC A Q + VD+V G + + + R++ +
Sbjct: 94 GVWKSLKNKGREVIIGVGGCVASQEGEAIIKRAPFVDLVFGPQTLHRLPELIRARREQKR 153
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
QV + K D E + AF+ I +GC+++C++C++PYTRG+ S+ + V
Sbjct: 154 PQVDISFPEIEKF--DRLPEPRADGASAFVSIMEGCSKYCSFCVVPYTRGTEVSRPFEDV 211
Query: 177 LDEIRSLAEHGFKEIILSGIHTASYGVDLD--EKVTLVDLLEEIEKIDGIERVRIGSIDP 234
+ E+ LA G +EI L G + +Y E L L+ I +IDG+ R+R + P
Sbjct: 212 VVEVAQLAAQGVREINLLGQNVNAYRGPYGDGEFADLGLLIRTIAEIDGVGRIRFTTSHP 271
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
F++ ++ + +L HL +Q+G D L M R YTA E++ + LR D+S
Sbjct: 272 LEFSDSLIDAFRDVPQLANFLHLPVQAGSDRVLSAMKRGYTALEFKSKIRKLRAVRPDIS 331
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
I++D IVGFPG ++ I + F YS R GT A ++E+ + +K R
Sbjct: 332 ISSDFIVGFPGETDADFEKTMKLIEDIGFDHSFSFIYSRRPGTPAADLEDTISDAEKHAR 391
Query: 355 SKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSGK 414
+L E + +EK +G V VL E TG T N VN A + G+
Sbjct: 392 LSRLQERINAHAAGISEKMVGTVQTVLVEGPSRKNPNELTGKTENMRSVNFPAPARLIGQ 451
Query: 415 ILNVKI 420
++V I
Sbjct: 452 FVDVVI 457
>MIAB_SHEPA RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 475
Score = 186 bits (472), Expect = 3e-49
Identities = 137/444 (30%), Positives = 216/444 (48%), Gaps = 18/444 (4%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVR-EGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S M + EGY + D E ADV ++NTCS+ +K +
Sbjct: 4 KLHIKTWGCQMNEYDSSKMADLLDEYEGYTLTDNAEEADVLLLNTCSIREKAQEKVFHQL 63
Query: 61 GR---ARRQNPEAIIAVVGCYA-QIAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR + + P II V GC A Q + VD++ G + + +++ +D K
Sbjct: 64 GRWKTLKDKKPGLIIGVGGCVASQEGKAIKERAQCVDLIFGPQTLHRLPEMIDQIKDGKK 123
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
+ V K D E AF+ I +GC+++C++C++PYTRG S+ V
Sbjct: 124 AVIDVSFPEVEKF--DRLPEPRAEGPSAFVSIMEGCSKYCSFCVVPYTRGEEVSRPLDDV 181
Query: 177 LDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKV-TLVDLLEEIEKIDGIERVRIGSIDP 234
+ EI LAE G +E+ L G + +Y G D+++ T +LL + IDGI+R+R + P
Sbjct: 182 ILEIAQLAEQGVREVNLLGQNVNAYRGATHDDEICTFAELLRYVAAIDGIDRLRFTTSHP 241
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
FT+D++ +L HL +QSG D L +M R + A EY+ I+ LR D+
Sbjct: 242 IEFTQDIIDVYEDTPELVSFLHLPVQSGSDLILTQMKRGHMAIEYKSIIRRLRKARPDIL 301
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
I++D I+GFPG ++ I + F YS R GT A ++ + V ++K ER
Sbjct: 302 ISSDFIIGFPGESKQDFADTMKLIEDIQFDHSFSFIYSARPGTPAADLPDNVSLDEKKER 361
Query: 355 SKKLIELNRINEKA--FAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVS 412
I +RI ++A ++ + +G V +L E G T N VN +
Sbjct: 362 --LAILQDRITQQAMRYSRQMVGTVQRILVEGPSVKNPMELRGRTENSRVVNFEGLHEHI 419
Query: 413 GKILNVKI-----NSFEGEVAEGE 431
GK ++V+I NS G GE
Sbjct: 420 GKFVDVEIVDVYTNSLRGVFIRGE 443
>MIAB_METPP RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 457
Score = 186 bits (472), Expect = 3e-49
Identities = 132/443 (29%), Positives = 208/443 (46%), Gaps = 12/443 (2%)
Query: 2 KVAFATLGCRVNTYESEAMTEKF-VREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
KV T GC++N Y+S+ M + EGYE E AD+ + NTCSV +K +
Sbjct: 9 KVFIKTFGCQMNEYDSDKMADVLRAAEGYEPTTDVEQADLILFNTCSVREKAQEKVFSDL 68
Query: 61 GRARRQNPEAI-IAVVGCYA-QIAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQ 118
GR + + I V GC A Q + VDVV G + + + + + + Q
Sbjct: 69 GRVKHLKARGVKIGVGGCVASQEGAALIERAPYVDVVFGPQTLHRLPEMLARRDAQQRPQ 128
Query: 119 VKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLD 178
V + K F+ L + T AF+ I +GC+++C+YC++PYTRG S+ VL
Sbjct: 129 VDISFPEIEK-FDHLPPARVDGAT-AFVSIMEGCSKYCSYCVVPYTRGEEVSRPFDDVLT 186
Query: 179 EIRSLAEHGFKEIILSGIHTASYGVDLDEKVTLVD---LLEEIEKIDGIERVRIGSIDPT 235
E+ SLA+ G KE+ L G + Y + + + D LLE + +I GI R+R + P
Sbjct: 187 EVASLADQGVKEVTLLGQNVNGYRGRMGDTAEIADFALLLEYVAEIPGIARIRYTTSHPN 246
Query: 236 FFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSI 295
F++ ++ + +L H HL +Q G D L M R YT+ EY+ + LR D+S+
Sbjct: 247 EFSQRLIDVYARVPQLVNHLHLPVQHGSDRILSAMKRGYTSLEYKSSIRKLRAIRPDISL 306
Query: 296 TTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERS 355
+TD IVGFPG ++ + + F YS R GT A + + K R
Sbjct: 307 STDFIVGFPGETDDDHARTMKLIEDVGFDHSFSFIYSARPGTPAAALHDDAPRELKLARL 366
Query: 356 KKLIELNRINEKAFAEKYIGEVIDVLFE---EEVELGSG-VYTGYTRNYIKVNAKADCNV 411
+++ E N + + +G V +L E EL +G G T VN +
Sbjct: 367 QQVQEAIEANGRRIGQSRVGTVQRILVEGPSRRPELNAGHELMGRTECNRIVNFAGPARL 426
Query: 412 SGKILNVKINSFEGEVAEGEIVL 434
+G++++V I GE++L
Sbjct: 427 TGQLIDVTITEAYPHSLRGEVLL 449
>MIAB_HELP2 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 437
Score = 186 bits (472), Expect = 3e-49
Identities = 134/434 (30%), Positives = 216/434 (49%), Gaps = 22/434 (5%)
Query: 1 MKVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
MKV T+GC +N+ +SE + + + Y+ +MAD+ ++NTCSV ++K I
Sbjct: 1 MKVYIETMGCAMNSRDSEHLLSELSKLDYKETSDPKMADLILINTCSVREKPERKLFSEI 60
Query: 61 GR-ARRQNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQ 118
G+ A+ + P A I V GC A ++ V VLG+RN +I ++K ++
Sbjct: 61 GQFAKIKKPNAKIGVCGCTASHMGADILKKAPSVSFVLGARNVSKISQVIHK-----EKA 115
Query: 119 VKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLD 178
V+V + + E + R+ L I GC++ CAYC++P+TRG S +L
Sbjct: 116 VEVAIDYDESAYA-FEFFEKKAQIRSLLNISIGCDKKCAYCIVPHTRGKEISIPMDLILK 174
Query: 179 EIRSLAEHGFKEIILSGIHTASYGVDLDE---KVTLVDLLEEIEKIDGIERVRIGSIDPT 235
E LA +G KE++L G + +YGV KV DLL+++ +I GIER+R S P
Sbjct: 175 EAEKLANNGTKELMLLGQNVNNYGVRFSSEHAKVDFSDLLDKLSEIPGIERIRFTSPHPL 234
Query: 236 FFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSI 295
+ + R K+C H+ LQSG A LK M R Y+ + + + V L+ + +V I
Sbjct: 235 HMNDGFLERFAKNPKVCKSIHMPLQSGSSAVLKMMRRGYSKEWFLNRVERLKALVPEVGI 294
Query: 296 TTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERS 355
+TD+IVGFP L+++ + F YSPR T A + +V R
Sbjct: 295 STDIIVGFPNESDKDFEDTMEVLEKVRFDTLYSFIYSPRPFTEAGAWKERVPLEVSSSRL 354
Query: 356 KKLIELNR--INEKAFAEKYIGEVIDVLFEEEVELGSGVYTGY-----TRNYIKVNAKAD 408
++L ++ + EKA E +G+ VL E E+ + + G+ T +I+V K
Sbjct: 355 ERLQNRHKEILEEKAKLE--VGKTHVVLVENRREMDNQI-VGFEGRSDTGKFIEVTCKEK 411
Query: 409 CNVSGKILNVKINS 422
N G+++ V+I S
Sbjct: 412 RN-PGELVKVEIIS 424
>MIAB_SHESR RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 474
Score = 186 bits (471), Expect = 4e-49
Identities = 135/447 (30%), Positives = 217/447 (48%), Gaps = 24/447 (5%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVR-EGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S M + +GY + + E AD+ ++NTCS+ +K +
Sbjct: 4 KLHIKTWGCQMNEYDSSKMADLLGEYQGYTLTEEAEEADILLLNTCSIREKAQEKVFHQL 63
Query: 61 GR---ARRQNPEAIIAVVGCYAQIAPTEVSGIEG----VDVVLGSRNKGEIVYYVNKARD 113
GR + +NP+ II V GC +A E I+ VD++ G + + + + R
Sbjct: 64 GRWKTLKDKNPDLIIGVGGC---VASQEGKAIKDRAHCVDIIFGPQTLHRLPDMIEQVRR 120
Query: 114 EGKQQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDP 173
K + V K F+ L T AF+ I +GC+++C++C++PYTRG S+
Sbjct: 121 GEKAVIDVSFPEIEK-FDRLPEPRAEGPT-AFVSIMEGCSKYCSFCVVPYTRGEEVSRPS 178
Query: 174 KKVLDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKV-TLVDLLEEIEKIDGIERVRIGS 231
++ EI LAE G +E+ L G + +Y G D + T +LL + IDGI+R+R +
Sbjct: 179 DDIILEIAQLAEQGVREVNLLGQNVNAYRGATHDGGICTFAELLRYVAAIDGIDRIRFTT 238
Query: 232 IDPTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIE 291
P FT+D++ +L HL +QSG D L M R + A EY+ I+ LR
Sbjct: 239 SHPIEFTQDIIDVYEDTPELVSFLHLPVQSGSDRILTAMKRGHMAIEYKSIIRRLRKARP 298
Query: 292 DVSITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKK 351
D+ I++D I+GFPG ++ +A + F YS R GT A ++ + VD +K
Sbjct: 299 DIQISSDFIIGFPGETKEDFADTMKLIEDVAFDHSFSFIYSARPGTPAADLPDDVDMEEK 358
Query: 352 DERSKKLIELNRINEKA--FAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADC 409
+R I +RI ++A ++ +G V +L E G T N VN +
Sbjct: 359 KQR--LAILQDRITQQAMRYSRHMMGTVQRILVEGPSVKNPMELRGRTENNRVVNFEGQP 416
Query: 410 NVSGKILNVKI-----NSFEGEVAEGE 431
G ++V+I NS G+ GE
Sbjct: 417 KHIGSFVDVEIVDVYTNSLRGKFIRGE 443
>MIAB_ERYLH RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 446
Score = 186 bits (471), Expect = 4e-49
Identities = 123/383 (32%), Positives = 191/383 (49%), Gaps = 9/383 (2%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIGR---A 63
+ GC++N Y+ E M E +G E E AD+ V+NTC + K IGR
Sbjct: 14 SFGCQMNVYDGERMAEMLDEKGIEPAPEGEDADLVVLNTCHIREKAVDKVYSDIGRLTKG 73
Query: 64 RRQNPEAIIAVVGCYAQIAPTEVSG-IEGVDVVLGSRNKGEIVYYVNKARDEGKQQVKVG 122
+ Q +IAV GC AQ E+ V +V+G + + ++ A GK+
Sbjct: 74 KTQTKAPMIAVAGCVAQAEGEEIMARAPAVSMVVGPQAYHRLPGMIDAAV-AGKRSTDTD 132
Query: 123 AVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDEIRS 182
K F L + + AFL IQ+GC++FC YC++PYTRG+ S+ ++DE +
Sbjct: 133 MPADAK-FAALP-KRRKSAPSAFLTIQEGCDKFCTYCVVPYTRGAEISRPFSALIDEAKK 190
Query: 183 LAEHGFKEIILSGIHTASY-GVDLDEKVT-LVDLLEEIEKIDGIERVRIGSIDPTFFTED 240
L E G KEI L G + +++ G D + + L+ E+ K ++RVR + P ++
Sbjct: 191 LVEAGAKEITLLGQNVSAWTGEDAKGRALGMAGLIRELAKDPDLKRVRYTTSHPADMDDE 250
Query: 241 VVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITTDVI 300
++ ++KL P+ HL +QSG D LK MNR +TA+ Y ++ R D++++ D I
Sbjct: 251 LIATHGEVEKLMPYLHLPVQSGNDRVLKAMNRSHTAESYLRLLERFRAARPDLALSGDFI 310
Query: 301 VGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSKKLIE 360
VGFPG + + ++ + FKYSPR GT A ME QV DER ++L
Sbjct: 311 VGFPGETEAEFEDTLKIVDEVRYAQAYSFKYSPRPGTPAATMERQVPKEVMDERLQRLQA 370
Query: 361 LNRINEKAFAEKYIGEVIDVLFE 383
++ AF +G +VL E
Sbjct: 371 ALNRDQAAFNAGSVGRTCEVLVE 393
>MIAB_WOLPM RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 439
Score = 185 bits (470), Expect = 6e-49
Identities = 120/383 (31%), Positives = 191/383 (49%), Gaps = 5/383 (1%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIGRARRQ 66
T GC++N Y+S M G+ VV AD+ ++NTC + +K +G+
Sbjct: 8 TYGCQMNVYDSVLMENVIKPLGFNVVSDAGKADLVILNTCHIREKAAEKLYSELGKIHSL 67
Query: 67 NPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVKVGAVL 125
E I V GC AQ EV VD+V+G ++ + + KA K V
Sbjct: 68 RKEMTIVVAGCVAQAEGEEVFRRAPFVDIVVGPQSIATLPELIVKA-SRSKGHVINTDFP 126
Query: 126 KNKVFEDLKIEEYNNK--TRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDEIRSL 183
+ F+ L E Y N + AFL IQ+GC++FC +C++PYTRG+ S+ ++ E L
Sbjct: 127 EVAKFDKLPDECYGNSQGSSAFLAIQEGCDKFCTFCVVPYTRGAEYSRPVNEIFREALKL 186
Query: 184 AEHGFKEIILSGIHTASYGVDLDEKV-TLVDLLEEIEKIDGIERVRIGSIDPTFFTEDVV 242
+G EI L G + +Y + + +V L L+ I KI+ +ER+R + P E +
Sbjct: 187 VANGANEINLLGQNVNAYHGECEGEVWDLGKLISHIAKIEKLERIRYTTSHPRDMHESLY 246
Query: 243 RRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITTDVIVG 302
KL P HL +QSG + L MNR++TA+EY +I++ R ++ ++D IVG
Sbjct: 247 LAHAEEPKLMPFVHLPVQSGSNKILHAMNRKHTAEEYLEIIDRFRKLKPEIEFSSDFIVG 306
Query: 303 FPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSKKLIELN 362
FPG ++++ ++ + FKYSPR GT ++QV K ER +L +L
Sbjct: 307 FPGETEKDFEETMKLVEKVRYAQAYSFKYSPRPGTPGAERKDQVPEEVKTERLLRLQKLI 366
Query: 363 RINEKAFAEKYIGEVIDVLFEEE 385
+ F + +G+ I VLF ++
Sbjct: 367 SKQQLEFNQSMVGKTIPVLFSDK 389
>MIAB_PROM4 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 467
Score = 185 bits (470), Expect = 6e-49
Identities = 132/442 (29%), Positives = 202/442 (45%), Gaps = 20/442 (4%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIGRA--- 63
T GC++N +SE M R GY+ + AD+ + NTC++ + ++K +GR
Sbjct: 28 TFGCQMNKADSERMAGILERMGYKQAEEELQADLVLYNTCTIRDNAEQKVYSYLGRQALR 87
Query: 64 RRQNPEAIIAVVGCYAQI-APTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVKVG 122
++ P+ + V GC AQ + + + +D+V+G ++ + +NK E QQV
Sbjct: 88 KKLFPQLKLIVAGCVAQQEGESLLRRVPELDLVMGPQHANRLETLLNKV--ETGQQVLAT 145
Query: 123 AVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDEIRS 182
L + EDL ++ A++ + GCN C YC++P RG S+ P + EI +
Sbjct: 146 EELY--IIEDLTTARRDSSICAWVNVIYGCNERCTYCVVPSVRGKEQSRTPPAIKREIEN 203
Query: 183 LAEHGFKEIILSGIHTASYGVDLD-------EKVTLVDLLEEIEKIDGIERVRIGSIDPT 235
LA GFKE+ L G + +YG DL +K TL DLL I I+GIER+R + P
Sbjct: 204 LANTGFKEVTLLGQNIDAYGRDLPGTTPQGRKKNTLTDLLYYIHDIEGIERIRFATSHPR 263
Query: 236 FFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSI 295
+FT ++ L K+C HFH+ QSG D L +M + + + SI
Sbjct: 264 YFTSRLIEACAELPKVCEHFHIPFQSGDDEILNQMXXXXXXXXXXXXXXXXXELMPNASI 323
Query: 296 TTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERS 355
T DVIV FPG ++ I + + YSPR T A NQ+ K ER
Sbjct: 324 TADVIVAFPGETNEQFQRSLSLVEEIGFDQLNTAAYSPRPNTPAALWPNQIPEEIKIERL 383
Query: 356 KKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRN-----YIKVNAKADCN 410
+KL L I K +Y V +VL E G TR + KV+ + +
Sbjct: 384 RKLNSLVEIKAKERNVRYKDRVEEVLAENTNPKDKQQLMGRTRTNRLTFFPKVDNQGNSY 443
Query: 411 VSGKILNVKINSFEGEVAEGEI 432
G ++ V++ G I
Sbjct: 444 KPGDLVKVRVKDIRAFSLSGVI 465
>MIAB_GLUDA RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 480
Score = 185 bits (470), Expect = 6e-49
Identities = 131/440 (29%), Positives = 206/440 (46%), Gaps = 18/440 (4%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIGRAR-- 64
T GC++N Y+S MT+ GY VD + AD+ ++NTC + + +K +GR R
Sbjct: 37 TWGCQMNVYDSARMTDVLRPLGYHPVDTPDTADMVILNTCHIRDRAAEKVFSELGRLRLV 96
Query: 65 -----RQNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQ 118
+ + ++AV GC AQ E+ + VD+VLG + + V
Sbjct: 97 KEARATEGQQTVLAVAGCVAQAEGKEILARAPFVDIVLGPQTYHRLPEMVXXXXXXXXXV 156
Query: 119 VKVGAVLKNKV--FEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
+ + K D + + +FL IQ+GC++FC++C++PYTRG+ S+ V
Sbjct: 157 IDTDFPAEQKFDFLPDAQAPQSPGGITSFLTIQEGCDKFCSFCVVPYTRGAEASRPVASV 216
Query: 177 LDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKVT-LVDLLEEIEKIDGIERVRIGSIDP 234
L E R + E G +EI L G + +Y G D +V L L E + I G+ R+R + P
Sbjct: 217 LREARRMVECGAREITLLGQNVNAYHGEGPDGRVWGLARLAEALAAIPGLARIRYTTSHP 276
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
D++ L L P HL +QSG D L MNR +TA +Y D+V LRD D++
Sbjct: 277 RDMDADLIAAHRDLPALMPFLHLPVQSGSDRILDAMNRGHTAADYRDLVLRLRDARPDIA 336
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
+++D IVG PG ++ + ++ FKYSPR GT A QV + K
Sbjct: 337 LSSDFIVGHPGETDADFEATLQLIRDVGFAQAFSFKYSPRPGTPAAGAPLQVAEDVKXXX 396
Query: 355 SKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSGK 414
F + +G V+ VLF +G +G + V+ + ++ G+
Sbjct: 397 XXXXXXXXXXXXXXFNDGTVGHVVPVLFTGHGR-KAGQLSGRSPYLQPVHVEGPDSLIGQ 455
Query: 415 ILNVKI-----NSFEGEVAE 429
I NV+I NS G + +
Sbjct: 456 IANVEIRERYTNSLSGTLVQ 475
>MIAB_THET8 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 465
Score = 185 bits (469), Expect = 7e-49
Identities = 131/445 (29%), Positives = 203/445 (45%), Gaps = 30/445 (6%)
Query: 1 MKVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
M+ T GC++N Y+S + + V G+E+VD E AD +VNTC+V +K R ++
Sbjct: 26 MRAHIITYGCQMNEYDSHLVASELVSLGWELVDSVEEADFVLVNTCAVRGKPVEKVRSLL 85
Query: 61 GRARRQNPEA--IIAVVGCYAQIAPTEVSGIE-GVDVVLGSRNKGEIVYYVNKARDEGKQ 117
G+ R++ +I ++GC AQ+ + + GVDV+LG G
Sbjct: 86 GQLRKEKERRGLLIGMMGCLAQLDEGQQMAKKFGVDVLLGP----------------GAL 129
Query: 118 QVKVGAVLKNKVFEDLKIEEY---------NNKTRAFLKIQDGCNRFCAYCLIPYTRGSV 168
A+ N+ F DL E A + I GCN C YC++P TRG
Sbjct: 130 TSLPEALKANERFWDLTFREDVLDYIPPPPKGALSAHVTIIRGCNHHCTYCIVPTTRGPE 189
Query: 169 CSKDPKKVLDEIRSLAEHGFKEIILSGIHTASYGVDLDEKVTLVDLLEEIEKIDGIERVR 228
S+ P +L EI L + G E+ L G + SYG D + +LL + + GI RVR
Sbjct: 190 VSRHPDLILKEIELLKQAGVVEVTLLGQNVNSYGKDQPGFPSFAELLRMVGGM-GIPRVR 248
Query: 229 IGSIDPTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRD 288
+ P FT+D++ I +C + HL +QSG D L+RM R Y Y + + +R+
Sbjct: 249 FLTSHPVNFTDDIIEAIAETPAICRYIHLPVQSGSDRVLRRMAREYRRAHYLERIRKIRE 308
Query: 289 KIEDVSITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDG 348
+ D ++TD+IVGFPG + + ++F YSPR GT A +
Sbjct: 309 ALPDAVLSTDIIVGFPGETEEDFQETLSLYDEVGYDQAYMFIYSPRPGTPAYKHFQDLPR 368
Query: 349 NKKDERSKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKAD 408
K ER +LIE + +++G+ ++VL E + G G+ R V A
Sbjct: 369 EVKVERLMRLIEKQKEWSYRRNLEWVGKTVEVLVRGEAK-EEGFVQGHDRGNHPVLVPAS 427
Query: 409 CNVSGKILNVKINSFEGEVAEGEIV 433
+ V+I + GE+V
Sbjct: 428 QAPVPGLYQVEIKQATPHLLFGEVV 452
>MIAB_THET2 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 465
Score = 185 bits (469), Expect = 7e-49
Identities = 131/445 (29%), Positives = 203/445 (45%), Gaps = 30/445 (6%)
Query: 1 MKVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
M+ T GC++N Y+S + + V G+E+VD E AD +VNTC+V +K R ++
Sbjct: 26 MRAHIITYGCQMNEYDSHLVASELVSLGWELVDSVEEADFVLVNTCAVRGKPVEKVRSLL 85
Query: 61 GRARRQNPEA--IIAVVGCYAQIAPTEVSGIE-GVDVVLGSRNKGEIVYYVNKARDEGKQ 117
G+ R++ +I ++GC AQ+ + + GVDV+LG G
Sbjct: 86 GQLRKEKERRGLLIGMMGCLAQLDEGQQMAKKFGVDVLLGP----------------GAL 129
Query: 118 QVKVGAVLKNKVFEDLKIEEY---------NNKTRAFLKIQDGCNRFCAYCLIPYTRGSV 168
A+ N+ F DL E A + I GCN C YC++P TRG
Sbjct: 130 TSLPEALKANERFFDLTFREDVLDYIPPPPKGALSAHVTIIRGCNHHCTYCIVPTTRGPE 189
Query: 169 CSKDPKKVLDEIRSLAEHGFKEIILSGIHTASYGVDLDEKVTLVDLLEEIEKIDGIERVR 228
S+ P +L EI L + G E+ L G + SYG D + +LL + + GI RVR
Sbjct: 190 VSRHPDLILKEIELLKQAGVVEVTLLGQNVNSYGKDQPGFPSFAELLRMVGGM-GIPRVR 248
Query: 229 IGSIDPTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRD 288
+ P FT+D++ I +C + HL +QSG D L+RM R Y Y + + +R+
Sbjct: 249 FLTSHPVNFTDDIIEAIAETPAICRYIHLPVQSGSDRVLRRMAREYRRAHYLERIRKIRE 308
Query: 289 KIEDVSITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDG 348
+ D ++TD+IVGFPG + + ++F YSPR GT A +
Sbjct: 309 ALPDAVLSTDIIVGFPGETEEDFQETLSLYDEVGYDQAYMFIYSPRPGTPAYKHFQDLPR 368
Query: 349 NKKDERSKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKAD 408
K ER +LIE + +++G+ ++VL E + G G+ R V A
Sbjct: 369 EVKVERLMRLIEKQKEWSYRRNLEWVGKTVEVLVRGEAK-EEGFVQGHDRGNHPVLVPAS 427
Query: 409 CNVSGKILNVKINSFEGEVAEGEIV 433
+ V+I + GE+V
Sbjct: 428 QAPVPGLYQVEIKQATPHLLFGEVV 452
>MIAB_SHEFN RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 474
Score = 185 bits (469), Expect = 7e-49
Identities = 132/444 (29%), Positives = 217/444 (48%), Gaps = 18/444 (4%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVR-EGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S M + +GY + + ADV ++NTCS+ +K +
Sbjct: 4 KLHIKTWGCQMNEYDSSKMADLLDDYQGYTLTEDASEADVLLLNTCSIREKAQEKVFHQL 63
Query: 61 GR---ARRQNPEAIIAVVGCYAQIAPTEVSG-IEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR + +NP II V GC A + + VD++ G + + + + + K
Sbjct: 64 GRWKSLKDKNPNLIIGVGGCVASQEGKAIKDRAQCVDIIFGPQTLHRLPEMIEQIQRGEK 123
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
+ + K F+ L + T AF+ I +GC+++C++C++PYTRG S+ V
Sbjct: 124 AVIDISFPEIEK-FDRLPEPRADGPT-AFVSIMEGCSKYCSFCVVPYTRGEEVSRPLDDV 181
Query: 177 LDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKV-TLVDLLEEIEKIDGIERVRIGSIDP 234
+ E+ LA G +E+ L G + +Y G D+++ T +LL + IDGI+R+R + P
Sbjct: 182 ILEVAQLAAQGVREVNLLGQNVNAYRGATHDDEICTFAELLRLVASIDGIDRLRFTTSHP 241
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
FT+D++ +L HL +QSG D L +M R + A EY+ I+ LR D+
Sbjct: 242 IEFTQDIIDVYEDTPELVSFLHLPVQSGSDRILTQMKRGHMAIEYKSIIRRLRKARPDIQ 301
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
I++D I+GFPG ++ +A + F YS R GT A ++ + V +K +R
Sbjct: 302 ISSDFIIGFPGESKDDFADTMKLIEDVAFDHSFSFIYSARPGTPAADLPDDVSDEEKKQR 361
Query: 355 SKKLIELNRINEKA--FAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVS 412
I +RI ++A ++ + +G V +L E G T N VN +A
Sbjct: 362 --LAILQDRITQQAMRYSRQMLGTVQRILVEGPSVKNPMELRGRTENSRVVNFEAAHTHI 419
Query: 413 GKILNVKI-----NSFEGEVAEGE 431
G ++VKI NS GE GE
Sbjct: 420 GSFVDVKIVDVYTNSLRGEFVRGE 443
>MIAB_RHOPS RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 473
Score = 185 bits (469), Expect = 7e-49
Identities = 117/394 (29%), Positives = 196/394 (49%), Gaps = 12/394 (3%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIG 61
K+ + GC++N Y+++ M + EG+ + AD+ ++NTC + +K +G
Sbjct: 6 KLHIKSYGCQMNVYDAQRMVDVLAPEGFVETATVDDADLVILNTCHIREKASEKVYSELG 65
Query: 62 RARRQNPEAI-------IAVVGCYAQIAPTE-VSGIEGVDVVLGSRNKGEIVYYVNKARD 113
R R EA IAV GC AQ E V G ++ + + +A
Sbjct: 66 RLRLARDEAASSGRRMQIAVAGCVAQAEGEEIVRRAPXXXXXXGPQSYHHLPQLLARADQ 125
Query: 114 EGKQ-QVKVGAVLKNKVFEDLKIEEYNNK-TRAFLKIQDGCNRFCAYCLIPYTRGSVCSK 171
G+ + + K + E + AF+ +Q+GC++FC +C++PYTRG+ S+
Sbjct: 126 AGRALETEFPVEDKFGFLPQPRPETIRARGISAFVTVQEGCDKFCTFCVVPYTRGAEVSR 185
Query: 172 DPKKVLDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKV-TLVDLLEEIEKIDGIERVRI 229
++D+++ LA++G +EI L G + +Y G D + TL LL + + GI R+R
Sbjct: 186 PVSAIIDDVKRLADNGVREITLIGQNVNAYHGEGPDSRAWTLGRLLRRLAAVPGIVRLRY 245
Query: 230 GSIDPTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDK 289
+ P ++++ L L P HL +QSG D L MNR++TA +Y +++ R
Sbjct: 246 STSHPNDVDDELIEAHRDLDALMPFVHLPVQSGSDPILAAMNRKHTAADYRRVIDRFRAV 305
Query: 290 IEDVSITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGN 349
++ ++D IVGFPG + +I + + FKYSPR GT A +M+ V
Sbjct: 306 RPQIAFSSDFIVGFPGETEADFAATLALVTQIGYAGAYSFKYSPRPGTPAADMQEMVPAA 365
Query: 350 KKDERSKKLIELNRINEKAFAEKYIGEVIDVLFE 383
DER ++L +L + AF + IG+ +DVLFE
Sbjct: 366 VMDERLERLQQLIDSQQSAFNKAAIGQTVDVLFE 399
>MIAB_HELPJ RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 437
Score = 185 bits (469), Expect = 7e-49
Identities = 135/434 (31%), Positives = 215/434 (49%), Gaps = 22/434 (5%)
Query: 1 MKVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
MKV T+GC +N+ +SE + + + Y+ +MAD+ ++NTCSV ++K I
Sbjct: 1 MKVYIETMGCAMNSRDSEHLLSELSKLDYKETSDPKMADLILINTCSVREKPERKLFSEI 60
Query: 61 GR-ARRQNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQ 118
G+ A+ + P A I V GC A ++ V VLG+RN +I ++K ++
Sbjct: 61 GQFAKIKKPNAKIGVCGCTASHMGADILKKAPSVSFVLGARNVSKISQVIHK-----EKA 115
Query: 119 VKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLD 178
V+V + + E + R+ L I GC++ CAYC++P+TRG S +L
Sbjct: 116 VEVAIDYDESAYA-FEFFEKKAQIRSLLNISIGCDKKCAYCIVPHTRGKEISIPMDLILK 174
Query: 179 EIRSLAEHGFKEIILSGIHTASYGVDLDE---KVTLVDLLEEIEKIDGIERVRIGSIDPT 235
E LA +G KE++L G + +YGV KV DLL+++ +I GIER+R S P
Sbjct: 175 EAEKLANNGTKELMLLGQNVNNYGVRFSSEHAKVGFSDLLDKLSEIQGIERIRFTSPHPL 234
Query: 236 FFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSI 295
+ + R K+C H+ LQSG A LK M R Y+ + + + V L+ + +V I
Sbjct: 235 HMNDAFLERFAQNPKVCKSIHMPLQSGSSAVLKMMRRGYSKEWFLNRVERLKALVPEVGI 294
Query: 296 TTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERS 355
+TD+IVGFP L+++ + F YSPR T A + +V R
Sbjct: 295 STDIIVGFPNESDKDFEDTMEVLEKVRFDTLYSFIYSPRPFTEAGAWKERVPLEISSLRL 354
Query: 356 KKLIELNR--INEKAFAEKYIGEVIDVLFEEEVELGSGVYTGY-----TRNYIKVNAKAD 408
++L ++ + EKA E +G+ VL E E+ G G+ T +I+V K
Sbjct: 355 ERLQNRHKEILEEKAKLE--VGKTHVVLVENRREM-DGQIVGFEGRSDTGKFIEVTCKEK 411
Query: 409 CNVSGKILNVKINS 422
N G+++ V+I S
Sbjct: 412 KN-PGELVRVEIIS 424
>MIAB_SHEON RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 474
Score = 184 bits (468), Expect = 1e-48
Identities = 133/444 (29%), Positives = 215/444 (48%), Gaps = 18/444 (4%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVR-EGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S M + +GY + + E AD+ ++NTCS+ +K +
Sbjct: 4 KLHIKTWGCQMNEYDSSKMADLLGEYQGYTLTEEAEEADILLLNTCSIREKAQEKVFHQL 63
Query: 61 GR---ARRQNPEAIIAVVGCYAQIAPTEVSG-IEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR + +NP+ II V GC A + + VD++ G + + + + R K
Sbjct: 64 GRWKTLKDKNPDLIIGVGGCVASQEGKAIKDRAQCVDIIFGPQTLHRLPEMIEQVRRGEK 123
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
+ V K F+ L T AF+ I +GC+++C++C++PYTRG S+ +
Sbjct: 124 AVIDVSFPEIEK-FDRLPEPRAEGPT-AFVSIMEGCSKYCSFCVVPYTRGEEVSRPSDDI 181
Query: 177 LDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKV-TLVDLLEEIEKIDGIERVRIGSIDP 234
+ EI LAE G +E+ L G + +Y G D + T +LL + IDGI+R+R + P
Sbjct: 182 ILEIAQLAEQGVREVNLLGQNVNAYRGATHDGGICTFAELLRYVAAIDGIDRIRFTTSHP 241
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
FT+D++ +L HL +QSG D L M R + A EY+ I+ LR D+
Sbjct: 242 IEFTQDIIDVYEDTPELVSFLHLPVQSGSDRILTAMKRGHMAIEYKSIIRRLRKARPDIQ 301
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
I++D I+GFPG ++ +A + F YS R GT A ++ + VD +K +R
Sbjct: 302 ISSDFIIGFPGETQEDFADTMKLIEDVAFDHSFSFIYSARPGTPAADLPDDVDMEEKKQR 361
Query: 355 SKKLIELNRINEKA--FAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKA----- 407
I +RI ++A ++ +G V +L E G T N VN +
Sbjct: 362 --LAILQDRITQQAMRYSRHMMGTVQRILVEGPSVKNPMELRGRTENNRVVNFEGLPKHI 419
Query: 408 DCNVSGKILNVKINSFEGEVAEGE 431
V +I++V NS G+ GE
Sbjct: 420 GTFVDVEIVDVYTNSLRGKFIRGE 443
>MIAB_POLSJ RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 447
Score = 184 bits (468), Expect = 1e-48
Identities = 131/438 (29%), Positives = 202/438 (46%), Gaps = 8/438 (1%)
Query: 2 KVAFATLGCRVNTYESEAMTEKF-VREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
KV T GC++N Y+S+ M++ EGYE + AD+ + NTCSV +K +
Sbjct: 4 KVFIKTFGCQMNEYDSDKMSDVLHAAEGYEPTQNVDEADLILFNTCSVREKAQEKVFSDL 63
Query: 61 GRARRQNPEAI-IAVVGCYA-QIAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQ 118
GR + + + I V GC A Q + VDVV G + + + K K Q
Sbjct: 64 GRVKHLKEKGVLIGVGGCVASQEGAAIIQRAPYVDVVFGPQTLHRLPDLLAKRARLNKPQ 123
Query: 119 VKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLD 178
V + K F+ L + AF+ I +GC+++C+YC++PYTRG S+ VL
Sbjct: 124 VDISFPEIEK-FDHLPPARVEGAS-AFVSIMEGCSKYCSYCVVPYTRGEEVSRPFDDVLV 181
Query: 179 EIRSLAEHGFKEIILSGIHTASYGVDLDEKVTLVD---LLEEIEKIDGIERVRIGSIDPT 235
E+ LA+ G KE+ L G + +Y + + + D LLE + I GIER+R + P
Sbjct: 182 EVAGLADQGVKEVTLLGQNVNAYRGPMGDTAEIADFATLLEYVSDIPGIERIRYVTSHPN 241
Query: 236 FFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSI 295
FT+ ++ L KL H HL +Q G D L M R YTA EY+ + LR D+++
Sbjct: 242 EFTQSLIDAYARLPKLVNHLHLPVQHGSDRILMAMKRGYTAMEYKSTIRKLRAIRPDLAM 301
Query: 296 TTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERS 355
++D IVGFPG + + + F YSPR GT A + + K R
Sbjct: 302 SSDFIVGFPGETEDDFNKMMKLIDDVGYDTSFSFIYSPRPGTPAAALHDDTPHEVKLRRL 361
Query: 356 KKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSGKI 415
+ L + +A + +G V +L E G T VN K + G++
Sbjct: 362 QHLQATIDDSVRAISASRLGTVQRILVEGASRKDPTELMGRTECNRVVNFKGQPRLIGQM 421
Query: 416 LNVKINSFEGEVAEGEIV 433
+++ I GE++
Sbjct: 422 VDITITQATQRSLRGEVL 439
>MIAB_ALTMD RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 477
Score = 184 bits (468), Expect = 1e-48
Identities = 136/444 (30%), Positives = 214/444 (48%), Gaps = 18/444 (4%)
Query: 2 KVAFATLGCRVNTYESEAMTEKF-VREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+SE M + G+ E ADV ++NTCS+ +K +
Sbjct: 4 KLYIKTWGCQMNEYDSEKMADLLDSTHGFSAAQSAEEADVILLNTCSIREKAQEKVFHQL 63
Query: 61 GR---ARRQNPEAIIAVVGCYA-QIAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR ++ PE II V GC A Q T VD+V G + + +N+ + K
Sbjct: 64 GRWKTLKQDKPELIIGVGGCVASQEGDTIRQRAPFVDLVFGPQTLHRLPEMINELKGGAK 123
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
+ V K F+ L T AF+ I +GC+++C +C++PYTRG S+ V
Sbjct: 124 SVIDVSFPEIEK-FDRLPEPRAEGPT-AFVSIMEGCSKYCTFCVVPYTRGEEVSRPVDDV 181
Query: 177 LDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKVT-LVDLLEEIEKIDGIERVRIGSIDP 234
L EI LA G +E+ L G + +Y G + D + +LLE + IDGI+R+R + P
Sbjct: 182 LLEIAQLAGQGVREVNLLGQNVNAYRGENYDGTICRFSELLELVAAIDGIDRIRYTTSHP 241
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
FT+D++ ++ +L H HL +QSG D L M R +TA EY+ + L+ ++S
Sbjct: 242 VEFTDDIIDAYASIPELVDHLHLPVQSGSDRILNLMKRGHTALEYKSKMRKLKKIRPNIS 301
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
+++D I+GFPG ++ + + F YS R GT A + + V K +R
Sbjct: 302 LSSDFIIGFPGETDADFEATMDLIQAVDYDLSFSFIYSARPGTPAADAVDDVTEETKKQR 361
Query: 355 SKKLIELNRINEKA--FAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVS 412
L + RI ++A A +G +L E + +G T N VN + ++
Sbjct: 362 LHLLQQ--RITQQALRIARHMVGTEQRILVEGPSKKNPMELSGRTENNRVVNFEGTPDMI 419
Query: 413 GKILNVKI-----NSFEGEVAEGE 431
G+ ++V I NS GEV E
Sbjct: 420 GEFVDVNITDVFTNSLRGEVVRRE 443
>MIAB_SHESM RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 474
Score = 184 bits (467), Expect = 1e-48
Identities = 135/447 (30%), Positives = 217/447 (48%), Gaps = 24/447 (5%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVR-EGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S M + +GY + + E AD+ ++NTCS+ +K +
Sbjct: 4 KLHIKTWGCQMNEYDSSKMADLLGEYQGYTLTEEAEEADILLLNTCSIREKAQEKVFHQL 63
Query: 61 GR---ARRQNPEAIIAVVGCYAQIAPTEVSGIEG----VDVVLGSRNKGEIVYYVNKARD 113
GR + +NP+ II V GC +A E I+ VD++ G + + + + R
Sbjct: 64 GRWKTLKDKNPDLIIGVGGC---VASQEGKAIKDRAHCVDIIFGPQTLHRLPDMIEQVRR 120
Query: 114 EGKQQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDP 173
K + V K F+ L T AF+ I +GC+++C++C++PYTRG S+
Sbjct: 121 GEKAVIDVSFPEIEK-FDRLPEPRAEGPT-AFVSIMEGCSKYCSFCVVPYTRGEEVSRPS 178
Query: 174 KKVLDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKV-TLVDLLEEIEKIDGIERVRIGS 231
++ EI LAE G +E+ L G + +Y G D + T +LL + IDGI+R+R +
Sbjct: 179 DDIILEIAQLAEQGVREVNLLGQNVNAYRGATHDGGICTFAELLRYVAAIDGIDRIRFTT 238
Query: 232 IDPTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIE 291
P FT+D++ +L HL +QSG D L M R + A EY+ I+ LR
Sbjct: 239 SHPIEFTQDIIDVYEDTPELVSFLHLPVQSGSDRILTAMKRGHMAIEYKSIIRRLRKARP 298
Query: 292 DVSITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKK 351
D+ I++D I+GFPG ++ +A + F YS R GT A ++ + VD +K
Sbjct: 299 DIQISSDFIIGFPGETKEDFADTMKLIEDVAFDHSFSFIYSARPGTPAADLPDDVDMEEK 358
Query: 352 DERSKKLIELNRINEKA--FAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADC 409
+R I +RI ++A ++ +G V +L E G T N VN +
Sbjct: 359 KQR--LAILQDRITQQAMRYSRHMMGTVQRILVEGPSVKNPMELRGRTENNRVVNFEGLP 416
Query: 410 NVSGKILNVKI-----NSFEGEVAEGE 431
G ++V+I NS G+ GE
Sbjct: 417 KHIGSFVDVEIVDVYTNSLRGKFIRGE 443
>MIAB_RHOP2 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 468
Score = 184 bits (467), Expect = 1e-48
Identities = 126/447 (28%), Positives = 214/447 (47%), Gaps = 16/447 (3%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIG 61
K+ + GC++N Y+++ M + EG+ + AD+ ++NTC + +K +G
Sbjct: 6 KLHIKSYGCQMNVYDAQRMVDVLAPEGFVETASVDDADLVILNTCHIREKASEKVFSELG 65
Query: 62 RARRQNPEAI-------IAVVGCYAQIAPTEVSGIE-GVDVVLGSRNKGEIVYYVNKARD 113
R R EA I V GC AQ E+ + VDVV+G ++ + + +A
Sbjct: 66 RLRLARDEASRDGRRMQIVVAGCVAQAEGDEIVRRQPAVDVVVGPQSYHHLPRLLAQAAQ 125
Query: 114 EGKQ-QVKVGAVLKNKVFEDLKIEEYNNK-TRAFLKIQDGCNRFCAYCLIPYTRGSVCSK 171
G+ + + K + E + AF+ +Q+GC++FC +C++PYTRG+ S+
Sbjct: 126 TGRALETEFPIADKFGFLPQPQPEAIRARGISAFVTVQEGCDKFCTFCVVPYTRGAEVSR 185
Query: 172 DPKKVLDEIRSLAEHGFKEIILSGIHTASYGVDLDE--KVTLVDLLEEIEKIDGIERVRI 229
+L ++ LA+HG +E+ L G + +Y D + TL LL+ + + GI R+R
Sbjct: 186 PVAAILADVERLADHGVRELTLIGQNVNAYHGDGPDGRPWTLGRLLQRLAAVPGIVRLRY 245
Query: 230 GSIDPTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDK 289
+ P +D++ L L P HL +QSG D L MNR++TA +Y +++ R
Sbjct: 246 STSHPNDVDDDLIAAHRDLDALMPFVHLPVQSGSDRILAAMNRKHTAADYRRVIDRFRAV 305
Query: 290 IEDVSITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGN 349
+++ ++D IVGFPG + +I + + FKYSPR GT
Sbjct: 306 RPEIAFSSDFIVGFPGETDADFEATLALVTQIGYAGAYSFKYSPRPGTXXXXXXXXXXXX 365
Query: 350 KKDERSKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVY--TGYTR--NYIKVNA 405
DER ++L ++ + AF IG +DVLFE + T Y + +
Sbjct: 366 XXDERLERLQQVIDAQQSAFNRAAIGRTVDVLFERAGRKPGQIVGRTAYLQPAHVFPPAG 425
Query: 406 KADCNVSGKILNVKINSFEGEVAEGEI 432
A ++ G+IL V+++S E GE+
Sbjct: 426 MAPDSLVGQILPVRVDSLERYSLLGEL 452
>MIAB_HELPH RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 437
Score = 184 bits (467), Expect = 1e-48
Identities = 133/434 (30%), Positives = 217/434 (50%), Gaps = 22/434 (5%)
Query: 1 MKVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
MKV T+GC +N+ +SE + + + Y+ + ++AD+ ++NTCSV ++K I
Sbjct: 1 MKVYIETMGCAMNSRDSEHLLSELSKLDYKETNDPKIADLILINTCSVREKPERKLFSEI 60
Query: 61 GR-ARRQNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQ 118
G+ A+ + P A I V GC A ++ V VLG+RN +I ++K ++
Sbjct: 61 GQFAKIKKPNAKIGVCGCTASHMGADILKKAPSVSFVLGARNVSKISQVIHK-----EKA 115
Query: 119 VKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLD 178
V+V + + E + R+ L I GC++ CAYC++P+TRG S +L
Sbjct: 116 VEVAIDYDESAYA-FEFFEKKAQIRSLLNISIGCDKKCAYCIVPHTRGKEISIPMDLILK 174
Query: 179 EIRSLAEHGFKEIILSGIHTASYGVDLDE---KVTLVDLLEEIEKIDGIERVRIGSIDPT 235
E LA +G KE++L G + +YGV KV DLL+++ +I GIER+R S P
Sbjct: 175 EAEKLANNGTKELMLLGQNVNNYGVRFSSEHAKVDFSDLLDKLSEIQGIERIRFTSPHPL 234
Query: 236 FFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSI 295
+ + R K+C H+ LQSG A LK M R Y+ + + + V L+ + +V I
Sbjct: 235 HMNDGFLERFAKNPKVCKSIHMPLQSGSSAVLKMMRRGYSKEWFLNRVERLKALVPEVGI 294
Query: 296 TTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERS 355
+TD+IVGFP L+++ + F YSPR T A + +V R
Sbjct: 295 STDIIVGFPNESDKDFEDTMEVLEKVRFDTLYSFIYSPRPFTEAGAWKERVPLEVSYSRL 354
Query: 356 KKLIELNR--INEKAFAEKYIGEVIDVLFEEEVELGSGVYTGY-----TRNYIKVNAKAD 408
++L ++ + EKA E +G+ VL E E+ + + G+ T +I+V K
Sbjct: 355 ERLQNRHKEILEEKAKLE--VGKTHVVLVENRREMDNQI-VGFEGRSDTGKFIEVTCKEK 411
Query: 409 CNVSGKILNVKINS 422
N G+++ V+I S
Sbjct: 412 RN-PGELVKVEIIS 424
>MIAB_STRGG RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 506
Score = 184 bits (466), Expect = 2e-48
Identities = 124/400 (31%), Positives = 191/400 (47%), Gaps = 16/400 (4%)
Query: 7 TLGCRVNTYESEAMTEKFVREGY----EVVDFNEMADVYVVNTCSVTNMGDKKSRQIIGR 62
T GC++N ++SE ++ GY E D + ADV V NTC+V D K +GR
Sbjct: 20 TYGCQMNVHDSERLSGLLEGAGYVRAPEGSDGD--ADVVVFNTCAVRENADNKLYGNLGR 77
Query: 63 A---RRQNPEAIIAVVGCYAQI-APTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQ 118
+ + P IAV GC AQ T V VDVV G+ N G++ + +AR + + Q
Sbjct: 78 LAPMKTKRPGMQIAVGGCLAQKDRDTIVKRAPWVDVVFGTHNIGKLPVLLERARVQEEAQ 137
Query: 119 VKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLD 178
+++ L+ F + A++ I GCN C +C++P RG + +L
Sbjct: 138 IEIAESLE--AFPSTLPTRRESAYAAWVSISVGCNNTCTFCIVPALRGKEKDRRTGDILA 195
Query: 179 EIRSLAEHGFKEIILSGIHTASYGVDLDEKVTLVDLLEEIEKIDGIERVRIGSIDPTFFT 238
EI +L G EI L G + +YG D+ ++ LL +I+G+ERVR S P FT
Sbjct: 196 EIEALVAEGVCEITLLGQNVNAYGSDIGDREAFSKLLRACGRIEGLERVRFTSPHPRDFT 255
Query: 239 EDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITTD 298
+DV+ + + P H+ +QSG D LK M R Y + + I+ +R + + +I+TD
Sbjct: 256 DDVIAAMAETPNVMPQLHMPMQSGSDTVLKAMRRSYRQERFLGIIEKVRAAMPEAAISTD 315
Query: 299 VIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSKKL 358
+IVGFPG ++ + F+YS R GT A +M+ Q+ ER +L
Sbjct: 316 IIVGFPGETEEDFEQTMHAVREARFANAFTFQYSKRPGTPAADMDGQIPKEVVQERYMRL 375
Query: 359 IELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTR 398
L +K +G +DV+ E G G G T+
Sbjct: 376 SALQEQISWDENKKQVGRTLDVMVAE----GEGRKDGATQ 411
>MIAB_HELAH RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 439
Score = 184 bits (466), Expect = 2e-48
Identities = 134/434 (30%), Positives = 215/434 (49%), Gaps = 22/434 (5%)
Query: 1 MKVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
+KV T+GC +N+ +SE + + + Y+ + ++AD+ ++NTCSV ++K I
Sbjct: 2 LKVYIETMGCAMNSRDSEHLLSELSKLDYKETNDPKIADLILINTCSVREKPERKLFSEI 61
Query: 61 GR-ARRQNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQ 118
G+ A+ + P A I V GC A ++ V VLG+RN +I ++K E +
Sbjct: 62 GQFAKIKKPNAKIGVCGCTASHMGADILKKSPSVSFVLGARNVSKISQVIHK---EKAVE 118
Query: 119 VKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLD 178
V + + FE E + R+ L I GC++ C YC++P+TRG S +L
Sbjct: 119 VAIDYDESSYAFEFF---EKKAEVRSLLNISIGCDKKCTYCIVPHTRGKEISIPMDLILK 175
Query: 179 EIRSLAEHGFKEIILSGIHTASYGVDLDE---KVTLVDLLEEIEKIDGIERVRIGSIDPT 235
E LA +G KE++L G + +YGV KV DLL+++ +I GIER+R S P
Sbjct: 176 EAEKLANNGTKELMLLGQNVNNYGVRFSSEHAKVNFSDLLDKLSEIPGIERIRFTSPHPL 235
Query: 236 FFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSI 295
++ + R K+C H+ LQSG A LK M R Y + + + V L+ + +V I
Sbjct: 236 HMNDEFLERFAKNPKVCKSIHMPLQSGSSAVLKMMRRGYNKEWFLNRVEKLKALVPEVGI 295
Query: 296 TTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERS 355
+TD+IVGFP L+++ + F YSPR T A + +V R
Sbjct: 296 STDIIVGFPNESDKDFEDTMEVLEKVCFDTLYSFIYSPRPFTEAGAWKERVPLEVSSLRL 355
Query: 356 KKLIELNR--INEKAFAEKYIGEVIDVLFEEEVELGSGVYTGY-----TRNYIKVNAKAD 408
++L ++ + EKA E +G+ VL E E+ G G+ T +I+V K
Sbjct: 356 ERLQNRHKEILEEKARLE--VGKTHVVLVENRHEV-DGQIVGFEGRSDTGKFIEVTCKEK 412
Query: 409 CNVSGKILNVKINS 422
N G+++ V+I S
Sbjct: 413 RN-PGELVEVEIIS 425
>MIAB_AERS4 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 477
Score = 184 bits (466), Expect = 2e-48
Identities = 131/442 (29%), Positives = 205/442 (46%), Gaps = 14/442 (3%)
Query: 2 KVAFATLGCRVNTYESEAMTEKF-VREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S M + GY + + E ADV ++NTCS+ +K +
Sbjct: 4 KLHIKTWGCQMNEYDSSKMADLLDASNGYTLTEEPEEADVLLLNTCSIREKAQEKVFHQL 63
Query: 61 GRARR---QNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR ++ P +I V GC A + S VD+V G + + + + ++
Sbjct: 64 GRWKKLKANKPGLVIGVGGCVASQEGENIRSRAPYVDIVFGPQTLHRLPAMIKQVQEGRG 123
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
QV + A + + F+ L T AF+ I +GC+++C++C++PYTRG S+ V
Sbjct: 124 AQVDI-AFPEIEKFDSLPEPRAEGAT-AFVSIMEGCSKYCSFCVVPYTRGEEVSRPMDDV 181
Query: 177 LDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKV-TLVDLLEEIEKIDGIERVRIGSIDP 234
L EI LAE G +E+ L G + +Y G D + + +LL + IDGI+R+R + P
Sbjct: 182 LYEIAQLAEQGVREVNLLGQNVNAYRGPTFDGSICSFAELLRLVAAIDGIDRIRYTTSHP 241
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
FT+D++ ++ HL +QSG D L M R +T EY+ + LR D++
Sbjct: 242 IEFTDDIIEVYKDTPEVVSFLHLPVQSGSDRILTMMKRPHTVLEYKSKIRRLRAARPDIT 301
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
I++D IVGFP ++ I + F YSPR GT A +M + VD K R
Sbjct: 302 ISSDFIVGFPNETDEDFEATMKLIEEINFDMSFSFIYSPRPGTPAADMPDDVDMEVKKVR 361
Query: 355 SKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSG- 413
+L + +G +L E L G T N VN + + G
Sbjct: 362 LARLQHVINNQSMQIGRAMLGSTQRILVEGPSRLDPMQLCGRTENNRVVNFEGAHTLIGG 421
Query: 414 ----KILNVKINSFEGEVAEGE 431
+I V+ NS G GE
Sbjct: 422 FADVEITEVRPNSLRGRFIRGE 443
>MIAB_SHEDO RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 474
Score = 183 bits (465), Expect = 2e-48
Identities = 132/444 (29%), Positives = 217/444 (48%), Gaps = 18/444 (4%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVR-EGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S M + +GY + D ADV ++NTCS+ +K +
Sbjct: 4 KLHIKTWGCQMNEYDSSKMADLLDEYQGYTLTDDATEADVLLLNTCSIREKAQEKVFHQL 63
Query: 61 GR---ARRQNPEAIIAVVGCYAQIAPTEVSG-IEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR + + P II V GC A + + VD++ G + + +++ + K
Sbjct: 64 GRWKTLKDKKPGLIIGVGGCVASQEGKAIKDRAQCVDIIFGPQTLHRLPEMIDQVQRGEK 123
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
+ V K F+ L + T AF+ I +GC+++C++C++PYTRG S+ +
Sbjct: 124 VVIDVSFPEIEK-FDRLPEPRADGPT-AFVSIMEGCSKYCSFCVVPYTRGEEVSRPQDDI 181
Query: 177 LDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKV-TLVDLLEEIEKIDGIERVRIGSIDP 234
+ EI LAE G +E+ L G + +Y G D+++ T +LL + IDGI+R+R + P
Sbjct: 182 ILEIAQLAEQGVREVNLLGQNVNAYRGATHDDQICTFAELLRLVASIDGIDRIRFTTSHP 241
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
FT+D++ +L HL +QSG D L +M R + A EY+ I+ LR E +
Sbjct: 242 IEFTQDIIDVYEDTPELVSFLHLPVQSGSDRILTQMKRGHMAIEYKSIIRRLRKAREGIQ 301
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
I++D I+GFPG ++ +A + F YS R GT A ++ + V +K +R
Sbjct: 302 ISSDFIIGFPGESAEDFADTMKLIEDVAFDHSFSFIYSARPGTPAADLPDDVSDEEKKQR 361
Query: 355 SKKLIELNRINEKA--FAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVS 412
I +RI ++A ++ + +G V +L E G T N VN +A
Sbjct: 362 --LAILQDRITQQAMRYSRQMLGTVQRILVEGPSVKNPMELRGRTENNRVVNFEAPHTHI 419
Query: 413 GKILNVKI-----NSFEGEVAEGE 431
G ++V+I NS G GE
Sbjct: 420 GSFVDVEIVDVYTNSLRGIFIRGE 443
>MIAB_RALME RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 450
Score = 183 bits (465), Expect = 2e-48
Identities = 135/449 (30%), Positives = 208/449 (46%), Gaps = 19/449 (4%)
Query: 2 KVAFATLGCRVNTYESEAMTEKF-VREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
KV T GC++N Y+S+ M + +G E D E ADV + NTCS+ +K +
Sbjct: 3 KVFVKTYGCQMNEYDSDKMVDVLNATQGMEPTDNVEDADVILFNTCSIREKAQEKVFSEL 62
Query: 61 GRARR---QNPEAIIAVVGCYA-QIAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR + P+ +I V GC A Q + VS VDVV G + + +N+ + G
Sbjct: 63 GRMKALKAAKPDLVIGVGGCVASQEGASIVSRAPYVDVVFGPQTLHRLPDLINRRQQTGL 122
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
QV + K F+ L + + AF+ I +GC+++C+YC++PYTRG S+ + V
Sbjct: 123 SQVDISFPEIEK-FDHLPPARVDGPS-AFVSIMEGCSKYCSYCVVPYTRGEEVSRPFEDV 180
Query: 177 LDEIRSLAEHGFKEIILSGIHTASYGVDLDEKVTLVD---LLEEIEKIDGIERVRIGSID 233
L E+ LAE G +E+ L G + +Y + + D L+E + I GIER+R +
Sbjct: 181 LAEVAGLAEQGVREVTLLGQNVNAYRGAMGGTSEIADFALLIEYVADIPGIERIRYTTSH 240
Query: 234 PTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDV 293
P FT ++ KL H HL +Q D L M R Y+ EY+ I+ LR D+
Sbjct: 241 PKEFTSRLIELYGRCDKLVNHLHLPVQHASDRVLAGMKRGYSVLEYKSIIRRLRALRPDM 300
Query: 294 SITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDE 353
S+++D IVGFPG + I + F +SPR GT A N+ + K
Sbjct: 301 SMSSDFIVGFPGETDADFDKLMAMVHEIGYDTSFSFIFSPRPGTPAANLHDDTPHEVKLR 360
Query: 354 RSKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVN--------A 405
R + L N + ++ +G V +L E G T N VN A
Sbjct: 361 RLQLLQATIEENVQRISQSMVGTVQRILVEGPARKDPTELHGRTENNRVVNFALPGVPQA 420
Query: 406 KADCNVSGKILNVKINSFEGEVAEGEIVL 434
+ D + G++++V + GE V+
Sbjct: 421 QRD-RMIGQMVDVNVTQAFPHSLRGEFVV 448
>MIAB_RALEJ RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 450
Score = 183 bits (465), Expect = 2e-48
Identities = 137/449 (30%), Positives = 209/449 (46%), Gaps = 19/449 (4%)
Query: 2 KVAFATLGCRVNTYESEAMTEKF-VREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
KV T GC++N Y+S+ M + +G E D E ADV + NTCSV +K +
Sbjct: 3 KVFVKTYGCQMNEYDSDKMVDVLNASQGLEPTDNVEDADVILFNTCSVREKAQEKVFSEL 62
Query: 61 GRARRQN---PEAIIAVVGCYA-QIAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR + P+ +I V GC A Q + VS VDVV G + + + + + G+
Sbjct: 63 GRMKALKAVKPDLVIGVGGCVASQEGASIVSRAPYVDVVFGPQTLHRLPDLIARRQRTGQ 122
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
QV + K F+ L + AF+ I +GC+++C+YC++PYTRG S+ + V
Sbjct: 123 SQVDISFPEIEK-FDHLPPARVEGPS-AFVSIMEGCSKYCSYCVVPYTRGEEVSRPFEDV 180
Query: 177 LDEIRSLAEHGFKEIILSGIHTASYGVDLDEKVTLVD---LLEEIEKIDGIERVRIGSID 233
L E+ LAE G +E+ L G + +Y + + D L+E + +I GIER+R +
Sbjct: 181 LAEVAGLAEQGVREVTLLGQNVNAYRGAMGGTSEIADFALLIEYVAEIPGIERIRYTTSH 240
Query: 234 PTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDV 293
P FT +V KL H HL +Q D L M R Y+ EY+ I+ LR D+
Sbjct: 241 PKEFTSRLVELYGRCDKLVNHLHLPVQHASDRVLMAMKRGYSVLEYKSIIRRLRMLRPDM 300
Query: 294 SITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDE 353
S+++D IVGFPG ++ + + F +SPR GT A N+ + K
Sbjct: 301 SMSSDFIVGFPGETDADFDKLMALIEEVGYDTSFSFIFSPRPGTPAANLHDDTPHEVKLR 360
Query: 354 RSKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVN--------A 405
R + L N + + +G V +L E G T N VN A
Sbjct: 361 RLQHLQATIEENVQRISRNMVGSVQRILVEGPSRKDPTELHGRTENNRVVNFALPGVPQA 420
Query: 406 KADCNVSGKILNVKINSFEGEVAEGEIVL 434
+ D + G++++V I GEIV+
Sbjct: 421 QRD-RMIGQMVDVSITQAFPHSLRGEIVV 448
>MIAB_BUCAI RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 439
Score = 183 bits (465), Expect = 2e-48
Identities = 130/435 (29%), Positives = 203/435 (46%), Gaps = 13/435 (2%)
Query: 7 TLGCRVNTYESEAMTEKFVREG-YEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIGR--- 62
T GC++N Y+S + + Y + E AD+ ++NTCS+ +K +GR
Sbjct: 8 TWGCQMNEYDSSMIITLLEKNNQYSLTKSAENADILILNTCSIREKAQEKVFHQLGRWXX 67
Query: 63 ARRQNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVKV 121
AV GC A E+ VD++ G++ + +++ + K + +
Sbjct: 68 XXXXXXXXXXAVGGCVATQEGKEIFKRANYVDIIFGTQTLHRLPKMIDEVEKKRKLSIDI 127
Query: 122 G--AVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDE 179
+ K K F K + Y A + I +GCN++C++C++PYTRG+ S+ VL E
Sbjct: 128 SFPKLEKFKYFLAPKKKGYT----ADISIMEGCNKYCSFCVVPYTRGNEISRPCDDVLFE 183
Query: 180 IRSLAEHGFKEIILSGIHTASY-GVDLDEKVTLV-DLLEEIEKIDGIERVRIGSIDPTFF 237
I LA+ G KEI L G + +Y G + KV +L+ + +IDGIER+R + +P F
Sbjct: 184 ISLLAKQGIKEINLLGQNVNAYQGPTFNGKVCYFSELIRLVAEIDGIERIRFTTSNPLEF 243
Query: 238 TEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITT 297
T+D++ KL HL +QSG + L M R YT ++Y I+ L D+ I++
Sbjct: 244 TDDIIEVYKDTPKLVSFLHLPVQSGSNKILNLMKRSYTTEDYTSIIKKLTIARPDIQISS 303
Query: 298 DVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSKK 357
D IVGFPG +K I + F YS R GT A NM + +D +K R
Sbjct: 304 DFIVGFPGESEIDFEKTIEFIKNINFDMSFSFIYSARPGTPASNMNDDLDLKEKKRRLYI 363
Query: 358 LIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSGKILN 417
L E I ++ K G + VL E + G T N V + + G+ +N
Sbjct: 364 LQERINIQTMLWSRKMFGSIQSVLVEGVSDKNIMDLYGRTENNRVVTFQGSSKMIGQFVN 423
Query: 418 VKINSFEGEVAEGEI 432
VKI +GE+
Sbjct: 424 VKIKKVHTHSLKGEL 438
>MIAB_ACIC1 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 497
Score = 183 bits (465), Expect = 2e-48
Identities = 122/384 (31%), Positives = 184/384 (47%), Gaps = 10/384 (2%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIGR---A 63
T GC++N ++SE + GY +E ADV V NTC+V D + +G
Sbjct: 32 TFGCQMNMHDSERIAGLLEAAGYVRAADDEPADVVVFNTCAVRENADNRLYGNLGLLLPV 91
Query: 64 RRQNPEAIIAVVGCYAQIAPTE-VSGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVKVG 122
++ P+ IAV GC AQ + V VDVV G+ N + + +AR + QV++
Sbjct: 92 KKAKPDMQIAVGGCLAQKDKGDIVRRAPWVDVVFGTHNIEALPVLLERARIAREAQVEIR 151
Query: 123 AVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDEIRS 182
L+ VF + A++ I GCN C +C++P RG + P +L EI++
Sbjct: 152 EALE--VFPSTLPSRRESVYAAWVAISVGCNNTCTFCIVPSLRGRERDRRPGDILREIQT 209
Query: 183 LAEHGFKEIILSGIHTASYGVDLDEKVTLVDLLEEIEKIDGIERVRIGSIDPTFFTEDVV 242
L G E+ L G + SYGVD ++ LL IDG+ERVR S P FT+DV+
Sbjct: 210 LVADGVLEVTLLGQNVNSYGVDFGDRQAFAKLLRACGTIDGLERVRFTSPHPRDFTDDVI 269
Query: 243 RRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITTDVIVG 302
+ + P H+ LQSG DA L+RM R Y ++Y DI++ +R VG
Sbjct: 270 AAMAETPNVMPQLHMPLQSGSDAVLRRMRRSYRKEKYLDIISRVRSXXXXXXXXXXXXVG 329
Query: 303 FPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSKKLIELN 362
FPG +++ + F+YS R GT A M +QV + +R +L+ L
Sbjct: 330 FPGETEDDFAETLDVVRKARFAGAFTFQYSKRPGTPAAEMPDQVPPDVVADRFARLVAL- 388
Query: 363 RINEKAFAEK--YIGEVIDVLFEE 384
+ + A E +G V++VL E
Sbjct: 389 -VEQIALEENQAQVGRVVEVLVAE 411
>RIMO_ALKMQ RecName: Full=Ribosomal protein S12
methylthiotransferase rimO; Short=S12
methylthiotransferase; Short=S12 MTTase;
Length = 446
Score = 183 bits (464), Expect = 3e-48
Identities = 114/390 (29%), Positives = 201/390 (51%), Gaps = 7/390 (1%)
Query: 1 MKVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKS-RQI 59
+ V +LGC N ++E M + GY++ + ADV +VNTC ++S +I
Sbjct: 3 LSVYVESLGCSKNLIDAEVMLGILNQYGYKLTNNEVKADVIIVNTCGFIEAAKEESINKI 62
Query: 60 IGRARRQNPEAIIAVV-GCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGKQ 117
I + +V GC + ++ + VD ++G+ EIV +++ +G++
Sbjct: 63 IELGQXXXXXXXXXIVAGCLGERYQKDLLEELPEVDAIVGTGGYHEIVKVIHQTM-KGQR 121
Query: 118 QVKVGAVLKNKVFED-LKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
V++G + N+ +++ L + A++KI DGC+ +C YC+IP RG S+ + +
Sbjct: 122 IVEIGDI--NRPYDETLPRIQTTASHSAYIKISDGCDNYCTYCIIPKLRGKYRSRKMENI 179
Query: 177 LDEIRSLAEHGFKEIILSGIHTASYGVDLDEKVTLVDLLEEIEKIDGIERVRIGSIDPTF 236
+ E ++LA +G KEIIL T YG+DL ++ L LL+++ +++GI+ +RI P
Sbjct: 180 IQEAQTLANNGVKEIILIAQDTTRYGIDLYDEYRLSALLDKLSEVEGIQWIRILYCYPEM 239
Query: 237 FTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSIT 296
T++++ I K+C + + +Q LK MNRR + +E ++ L+ + ++ I
Sbjct: 240 ITDELIATIKNNDKVCKYIDIPIQHCSTKILKLMNRRTSKEEIVSLIEKLKKNVPNIVIR 299
Query: 297 TDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSK 356
T +IVGFPG ++ I + +F YS +GT A + QV K+ R K
Sbjct: 300 TSIIVGFPGESEEDFNELKAFIEDIKFDRLGVFTYSQEEGTPAAQLAEQVPSELKESRQK 359
Query: 357 KLIELNRINEKAFAEKYIGEVIDVLFEEEV 386
L+EL + YIG+ I+VL EEE+
Sbjct: 360 ILMELQQRISLNKNRLYIGQSIEVLIEEEI 389
>MIAB_VIBCH RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 474
Score = 183 bits (464), Expect = 3e-48
Identities = 132/445 (29%), Positives = 209/445 (46%), Gaps = 20/445 (4%)
Query: 2 KVAFATLGCRVNTYESEAMTEKF-VREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S M + GYE+ + E ADV ++NTCS+ +K +
Sbjct: 4 KLLIKTWGCQMNEYDSSKMADLLNAANGYELTEIPEEADVLLLNTCSIREKAQEKVFHQL 63
Query: 61 GR---ARRQNPEAIIAVVGCYAQIAPTEVSGIEG----VDVVLGSRNKGEIVYYVNKARD 113
GR + + P +I V GC +A E I VDV+ G + + + +++
Sbjct: 64 GRWKTLKDKKPGVVIGVGGC---VATQEGDSIRDRAPYVDVIFGPQTLHRLPEMIKQSQT 120
Query: 114 EGKQQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDP 173
+ + K F+ L T AF+ I +GC+++C YC++PYTRG S+
Sbjct: 121 SDAPVMDISFPEIEK-FDRLPEPRAEGPT-AFVSIMEGCSKYCTYCVVPYTRGEEVSRPM 178
Query: 174 KKVLDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKV-TLVDLLEEIEKIDGIERVRIGS 231
VL EI LAE G +E+ L G + +Y G D + + +LL + IDGI+R+R +
Sbjct: 179 DDVLFEIAQLAEQGVREVNLLGQNVNAYRGATHDGGICSFAELLRLVATIDGIDRIRFTT 238
Query: 232 IDPTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIE 291
P FT+D++ +L HL +QSG D L M R +TA EY+ I+ LR
Sbjct: 239 SHPLEFTDDIIAVYEDTPELVSFLHLPVQSGSDRILTMMKRPHTAIEYKSIIRKLRKARP 298
Query: 292 DVSITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKK 351
D+ I++D IVGFPG ++ + + F +SPR GT A + + K
Sbjct: 299 DIQISSDFIVGFPGETDKDFQDTMKLIRDVDFDMSFSFIFSPRPGTPAADYPCDLSEEVK 358
Query: 352 DERSKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNV 411
ER +L + ++ +G +L E + G T N VN + ++
Sbjct: 359 KERLYELQQQINSQAMRYSRLMLGTEQRILVEGPSKKDLMELRGRTENNRVVNFEGSPDL 418
Query: 412 SGKILNVKI-----NSFEGEVAEGE 431
G+ ++VKI NS GE+ E
Sbjct: 419 IGQFVDVKIVDVFPNSLRGELLRTE 443
>MIAB_VIBC3 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 474
Score = 183 bits (464), Expect = 3e-48
Identities = 132/445 (29%), Positives = 209/445 (46%), Gaps = 20/445 (4%)
Query: 2 KVAFATLGCRVNTYESEAMTEKF-VREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S M + GYE+ + E ADV ++NTCS+ +K +
Sbjct: 4 KLLIKTWGCQMNEYDSSKMADLLNAANGYELTEIPEEADVLLLNTCSIREKAQEKVFHQL 63
Query: 61 GR---ARRQNPEAIIAVVGCYAQIAPTEVSGIEG----VDVVLGSRNKGEIVYYVNKARD 113
GR + + P +I V GC +A E I VDV+ G + + + +++
Sbjct: 64 GRWKTLKDKKPGVVIGVGGC---VATQEGDSIRDRAPYVDVIFGPQTLHRLPEMIKQSQT 120
Query: 114 EGKQQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDP 173
+ + K F+ L T AF+ I +GC+++C YC++PYTRG S+
Sbjct: 121 SDAPVMDISFPEIEK-FDRLPEPRAEGPT-AFVSIMEGCSKYCTYCVVPYTRGEEVSRPM 178
Query: 174 KKVLDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKV-TLVDLLEEIEKIDGIERVRIGS 231
VL EI LAE G +E+ L G + +Y G D + + +LL + IDGI+R+R +
Sbjct: 179 DDVLFEIAQLAEQGVREVNLLGQNVNAYRGATHDGGICSFAELLRLVATIDGIDRIRFTT 238
Query: 232 IDPTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIE 291
P FT+D++ +L HL +QSG D L M R +TA EY+ I+ LR
Sbjct: 239 SHPLEFTDDIIAVYEDTPELVSFLHLPVQSGSDRILTMMKRPHTAIEYKSIIRKLRKARP 298
Query: 292 DVSITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKK 351
D+ I++D IVGFPG ++ + + F +SPR GT A + + K
Sbjct: 299 DIQISSDFIVGFPGETDKDFQDTMKLIRDVDFDMSFSFIFSPRPGTPAADYPCDLSEEVK 358
Query: 352 DERSKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNV 411
ER +L + ++ +G +L E + G T N VN + ++
Sbjct: 359 KERLYELQQQINSQAMRYSRLMLGTEQRILVEGPSKKDLMELRGRTENNRVVNFEGSPDL 418
Query: 412 SGKILNVKI-----NSFEGEVAEGE 431
G+ ++VKI NS GE+ E
Sbjct: 419 IGQFVDVKIVDVFPNSLRGELLRTE 443
>MIAB_HELPY RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 437
Score = 183 bits (464), Expect = 3e-48
Identities = 132/434 (30%), Positives = 215/434 (49%), Gaps = 22/434 (5%)
Query: 1 MKVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
MKV T+GC +N+ +SE + + + Y+ + + AD+ ++NTCSV ++K I
Sbjct: 1 MKVYIETMGCAMNSRDSEHLLSELSKLDYKETNDPKTADLILINTCSVREKPERKLFSEI 60
Query: 61 GR-ARRQNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQ 118
G+ A+ + P A I V GC A ++ V VLG+RN +I ++K ++
Sbjct: 61 GQFAKIKKPNAKIGVCGCTASHMGADILKKAPSVSFVLGARNVSKISQVIHK-----EKA 115
Query: 119 VKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLD 178
V+V + + E + R+ L I GC++ CAYC++P+TRG S +L
Sbjct: 116 VEVAIDYDESAYA-FEFFEKKAQIRSLLNISIGCDKKCAYCIVPHTRGKEISIPMDLILK 174
Query: 179 EIRSLAEHGFKEIILSGIHTASYGVDLDE---KVTLVDLLEEIEKIDGIERVRIGSIDPT 235
E LA +G KE++L G + +YG KV DLL+++ +I GIER+R S P
Sbjct: 175 EAEKLANNGTKELMLLGQNVNNYGARFSSEHAKVDFSDLLDKLSEIQGIERIRFTSPHPL 234
Query: 236 FFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSI 295
+ + R K+C H+ LQSG A LK M R Y+ + + + V L+ + +V I
Sbjct: 235 HMNDGFLERFAKNPKVCKSIHMPLQSGSSAVLKMMRRGYSKEWFLNRVERLKALVPEVGI 294
Query: 296 TTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERS 355
+TD+IVGFP L+++ + F YSPR T A + +V R
Sbjct: 295 STDIIVGFPNESDKDFEDTMEVLEKVRFDTLYSFIYSPRPFTEAGAWKERVPLEVSSSRL 354
Query: 356 KKLIELNR--INEKAFAEKYIGEVIDVLFEEEVELGSGVYTGY-----TRNYIKVNAKAD 408
++L ++ + EKA E +G+ VL E E+ + + G+ T +I+V K
Sbjct: 355 ERLQNRHKEILEEKAKLE--VGKTHVVLVENRREMDNQI-VGFEGRSDTGKFIEVTCKEK 411
Query: 409 CNVSGKILNVKINS 422
N G+++ V+I S
Sbjct: 412 RN-PGELVKVEIIS 424
>MIAB_ARTS2 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 522
Score = 183 bits (464), Expect = 3e-48
Identities = 121/380 (31%), Positives = 192/380 (50%), Gaps = 9/380 (2%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIGR---A 63
T GC++N ++SE M GY V E ADV V NTC+V D K +G
Sbjct: 34 TFGCQMNVHDSERMAGMLEDAGY-VPASGENADVVVFNTCAVRENADNKLYGNLGMLAPV 92
Query: 64 RRQNPEAIIAVVGCYAQI-APTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVKVG 122
+ NP IAV GC AQ T + VD V G+ N G + +++AR + Q+++
Sbjct: 93 KAANPGMQIAVGGCLAQKDRETILKKAPWVDAVFGTHNVGALPALLDRARHNNEAQLEIL 152
Query: 123 AVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDEIRS 182
L VF + ++ ++ I GCN C +C++P RG + P +L EI++
Sbjct: 153 ESLD--VFPSTLPTKRDSVYSGWVSISVGCNNTCTFCIVPALRGKEKDRRPGDILAEIQA 210
Query: 183 LAEHGFKEIILSGIHTASYGVDLDEKVTLVDLLEEIEKIDGIERVRIGSIDPTFFTEDVV 242
L + G E+ L G + SYGV+ ++ LL +I G+ERVR S P FT+DV+
Sbjct: 211 LVDDGAIEVTLLGQNVNSYGVEFGDRQAFSKLLRACGEIQGLERVRFTSPHPAAFTDDVI 270
Query: 243 RRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITTDVIVG 302
+ + P H+ LQSG D LK M R Y + ++ I++ +R++I +I+TD+IVG
Sbjct: 271 DAMAETPNVMPQLHMPLQSGSDKVLKDMKRSYRSTKFLGILDKVRERIPHAAISTDIIVG 330
Query: 303 FPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSKKLIEL- 361
FPG +++ + F+YS R GT A ++ +Q+ ER ++L L
Sbjct: 331 FPGETEEDFQATLDVVEKSRFATAFTFQYSKRPGTPAADLPDQLPKAVVQERFERLTALQ 390
Query: 362 NRINEKAFAEKYIGEVIDVL 381
+RI + A + +G ++V+
Sbjct: 391 DRIAAEENA-RQLGRRVEVM 409
>MIAB_VIBPA RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 474
Score = 182 bits (463), Expect = 4e-48
Identities = 127/439 (28%), Positives = 203/439 (46%), Gaps = 9/439 (2%)
Query: 2 KVAFATLGCRVNTYESEAMTEKF-VREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S M + GYE+ + E ADV ++NTCS+ +K +
Sbjct: 4 KLLIKTWGCQMNEYDSSKMADLLNAANGYELTEEPEEADVLLLNTCSIREKAQEKVFHQL 63
Query: 61 GR---ARRQNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR + + P +I V GC A + VDV+ G + + + +++ +
Sbjct: 64 GRWKTLKDKKPGVVIGVGGCVATQEGDHIRERAPYVDVIFGPQTLHRLPEMIKQSQTDDA 123
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
+ + K F+ L T AF+ I +GC+++C YC++PYTRG S+ V
Sbjct: 124 PVMDISFPEIEK-FDRLPEPRAEGAT-AFVSIMEGCSKYCTYCVVPYTRGEEVSRPMDDV 181
Query: 177 LDEIRSLAEHGFKEIILSGIHTASYGVDLD--EKVTLVDLLEEIEKIDGIERVRIGSIDP 234
L EI LAE G +E+ L G + +Y + E + +LL + IDGI+R+R + P
Sbjct: 182 LFEIAQLAEQGVREVNLLGQNVNAYRGPMHDGEICSFAELLRLVASIDGIDRIRFTTSHP 241
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
FT+D++ +L HL +QSG D L M R +TA EY+ I+ LR D+
Sbjct: 242 LEFTDDIIAVYEDTPELVSFLHLPVQSGSDRILTMMKRPHTAIEYKSIIRKLRKARPDIQ 301
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
I++D IVGFPG +K + + F +SPR GT A + + K ER
Sbjct: 302 ISSDFIVGFPGETDKDFQDTMKLIKDVDFDMSFSFIFSPRPGTPAADYPCDIPEQVKKER 361
Query: 355 SKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSGK 414
+L + ++ + VL E + T N VN + ++ G+
Sbjct: 362 LYELQQTINAQAMRYSRLMLATEQRVLVEGPSKKNLMELRARTENNRVVNFEGSADLIGQ 421
Query: 415 ILNVKINSFEGEVAEGEIV 433
++VKI GE+V
Sbjct: 422 FVDVKITDVFANSLRGELV 440
>MIAB_SACD2 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 454
Score = 182 bits (463), Expect = 4e-48
Identities = 133/448 (29%), Positives = 216/448 (48%), Gaps = 24/448 (5%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVREGYEVV--DFNEMADVYVVNTCSVTNMGDKKSRQI 59
K+ T GC++N Y+S M + + +++V D E ADV +VNTCS+ +K
Sbjct: 12 KLYIQTHGCQMNEYDSSRMRD-LLGASHDMVPTDNPEEADVLLVNTCSIREKAQEKLFHQ 70
Query: 60 IGR---ARRQNPEAIIAVVGCYAQIAPTEVSG-IEGVDVVLGSRNKGEIVYYVNKARDEG 115
+GR + P+ +I V GC A +S VD++ G + + + R+ G
Sbjct: 71 LGRWKHLKEAKPDLVIGVGGCVASQEGDAISKRAPFVDLIFGPQTLHRLPEMIETPRENG 130
Query: 116 KQQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKK 175
V + K F++L E + T AF+ + +GC+++C +C++PYTRG S+
Sbjct: 131 AVVVDISFPEIEK-FDNLPEPEADGAT-AFVSVMEGCSKYCTFCVVPYTRGEEVSRPAVD 188
Query: 176 VLDEIRSLAEHGFKEIILSGIHTASYGVDLD-EKVTLVDLLEEIEKIDGIERVRIGSIDP 234
VL E+ LA G +E+ L G + +Y D E + L +L+ + IDGI+R+R + P
Sbjct: 189 VLREVTHLASQGVREVNLLGQNVNAYRDDSSGEVIDLAELIAAVADIDGIDRIRFTTSHP 248
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
FTE +++ + +L H HL +QSG D L M R +T EY+ + +R D+S
Sbjct: 249 MEFTESLIQAYAEIPELVSHLHLPVQSGSDRILSAMKRGHTCLEYKSKLRKIRALRPDIS 308
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
++D I+GFPG ++ I + F YSPR GT A ++ + K +R
Sbjct: 309 FSSDFIIGFPGETEADFAATMKLIEDIGFDNSFSFIYSPRPGTPAADLPDDTPEQVKKDR 368
Query: 355 SKKLIELNRINEKA--FAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCN-- 410
K I RI ++A + + +G VL + G +G T N VN + CN
Sbjct: 369 LK--ILQTRIIQQAQEISRRMVGNTERVLVTGYSKKDPGQLSGRTENNRVVNFR--CNQP 424
Query: 411 -VSGKILNVKI-----NSFEGEVAEGEI 432
+ GK ++ I NS G + E+
Sbjct: 425 ELIGKFADILIEEALPNSLRGVLLGSEL 452
>MIAB_PROM9 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 463
Score = 182 bits (463), Expect = 4e-48
Identities = 133/445 (29%), Positives = 209/445 (46%), Gaps = 26/445 (5%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIGR-ARR 65
T GC++N +SE M + GY D AD+ + NTC++ + + K +GR A+R
Sbjct: 25 TFGCQMNKADSERMAGTLEKMGYTRADDELNADLVLYNTCTIRDNAEHKVYSFLGRQAKR 84
Query: 66 QN--PEAIIAVVGCYAQI-APTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVKVG 122
++ P + V GC AQ + + + +D+V+G ++ + + K D G Q V
Sbjct: 85 KHKIPSLKLVVAGCLAQQEGESLLRRVPELDLVMGPQHVNNLENLLGKV-DLGNQ---VA 140
Query: 123 AVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDEIRS 182
A + + ED+ + ++ I GCN C+YC++P RG S+ P + EI+
Sbjct: 141 ATEETFISEDITNARRESSICGWVNIIYGCNERCSYCVVPSVRGKEQSRYPNAIKSEIQK 200
Query: 183 LAEHGFKEIILSGIHTASYGVDLD-------EKVTLVDLLEEIEKIDGIERVRIGSIDPT 235
LA+ FKEI L G + +YG DL ++ TL DLL I I+GI R+R + P
Sbjct: 201 LADDNFKEITLLGQNIDAYGRDLPGTTKEGRKENTLTDLLYYIHDINGISRIRFATSHPR 260
Query: 236 FFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSI 295
+F++ +++ L K+C HFH+ QSG D LK+M+R R + D SI
Sbjct: 261 YFSKRLIQACYELDKVCEHFHIPFQSGNDEILKQMSRGXXXXXXXXXXXXXRSLMPDASI 320
Query: 296 TTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERS 355
T D IV FPG + I + + YSPR T A NQ+ ++ +
Sbjct: 321 TADAIVAFPGETEQQYQDTLKLISDIGFDQVNTAAYSPRPNTPAAVWSNQL---SEEVKK 377
Query: 356 KKLIELNRINEKAFA---EKYIGEVIDVLFEEEVELGSGVYTGYTR----NYIKVNAKAD 408
+L E+N + EK ++YI + VL E S G TR ++K+
Sbjct: 378 TRLQEINALVEKTARNRNQRYINNIESVLIEGLNPKNSSQIMGRTRTNRLTFVKIPKNIQ 437
Query: 409 CNVS-GKILNVKINSFEGEVAEGEI 432
N S G ++V+IN GE+
Sbjct: 438 FNFSLGDEIDVRINEARPFSLTGEL 462
>MIAB_PELUB RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 446
Score = 182 bits (463), Expect = 4e-48
Identities = 124/439 (28%), Positives = 224/439 (51%), Gaps = 14/439 (3%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIG 61
K+ T GC++N Y+S + + + G+E + E A+ Y++NTC + + +K IG
Sbjct: 4 KIFIKTFGCQMNEYDSNRIFDTVKKIGFEKTEKYEDANCYLLNTCHIRDKAKEKVYHEIG 63
Query: 62 RAR---RQNPEAIIAVVGCYAQIAPTEVSGIEG-VDVVLGSRNKGEIVYYVNKARDEGKQ 117
R + R+ + I+ V GC AQ E+ E +D+V+G ++ +I +
Sbjct: 64 RVKKIFREKKKPIVVVAGCVAQAENQEMLKREPYIDIVIGPQSYHKINEAILNHLXXXXX 123
Query: 118 QVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVL 177
+K +I+ ++K +FL IQ+GC++FC +C++PYTRG S+ +++
Sbjct: 124 XXXXXFDTISKFNYLSQIKNKDSKVSSFLTIQEGCDKFCHFCVVPYTRGPEYSRPFDQII 183
Query: 178 DEIRSLAEHGFKEIILSGIHTASYGVDLD-EKVTLVDLLEEIEKIDGIERVRIGSIDPTF 236
+E + L + G KEIIL G + +Y D + +K L DLL +++ D +ER+R + P
Sbjct: 184 NEAKELVQSGAKEIILLGQNVNAYSYDEEGKKYRLSDLLIKLDSFDKLERIRYTTSHPKD 243
Query: 237 FTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSIT 296
T+D++ KL P HL +QSG + L MNR++T +EY + LR + +
Sbjct: 244 MTDDLINVYKTSSKLMPLVHLPVQSGSNKILNLMNRKHTIEEYLLVYEKLRKINPKIEFS 303
Query: 297 TDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSK 356
+D I+G+P ++++ ++ F +SPR GT A N+ VD KK ++
Sbjct: 304 SDFIIGYPEEDEQDFKMTMELIEKVKFINSYSFIFSPRPGTVAANL-TLVD-QKKSKQRL 361
Query: 357 KLIELNRINEKAFAEKYI-GEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSGKI 415
++I+ N + K + ++++VL E +++ G ++ G T V + GK+
Sbjct: 362 EIIQEKLFNNQIKKNKSLENKILNVLVENKMKDGIKLF-GRTEYMTSVIFDGNIENIGKL 420
Query: 416 LNVKI-----NSFEGEVAE 429
+ V+I NS G++ E
Sbjct: 421 VQVEIISSNQNSLFGKLTE 439
>MIAB_ERWT9 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 474
Score = 182 bits (463), Expect = 4e-48
Identities = 133/445 (29%), Positives = 214/445 (48%), Gaps = 20/445 (4%)
Query: 2 KVAFATLGCRVNTYESEAMTEKF-VREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S M + GY + + E ADV ++NTCS+ +K ++
Sbjct: 4 KLHIKTWGCQMNEYDSSKMADLLNSTHGYTLTEQAEDADVLLLNTCSIREKAQEKVFALL 63
Query: 61 GRARR---QNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR ++ NP+ II V GC A ++ VD+V G + + +N R
Sbjct: 64 GRWKKLKESNPDMIIGVGGCVASQEGAQIRQRASCVDIVFGPQTLHRLPEMINSVRGTRS 123
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
V V K D E + AF+ I +GCN++C +C++PYTRG S+ +
Sbjct: 124 PVVDVSFPEIEKF--DRMPEPRADGPTAFVSIMEGCNKYCTFCVVPYTRGEEVSRPSDDI 181
Query: 177 LDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKV-TLVDLLEEIEKIDGIERVRIGSIDP 234
L E+ LA G +E+ L G + +Y G D + + +LL + IDGI+R+R + P
Sbjct: 182 LFEVAQLAAQGVREVNLLGQNVNAYRGETFDGGICSFAELLRLVAAIDGIDRIRFTTSHP 241
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
F +D++ +L HL +QSG D L M R +TA EY+ I+ L ++
Sbjct: 242 IEFNDDIIDVYRDTPELVSFLHLPVQSGADRILTLMKRAHTALEYKAIIRKLLAARPNIQ 301
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
I++D I+GFPG + I ++ F YS R GT A ++ + V +DE+
Sbjct: 302 ISSDFIIGFPGETQADFEQTMKLIGEINFDISYSFIYSARPGTPAADLPDDV---SEDEK 358
Query: 355 SKKLIEL-NRINEK--AFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNV 411
++L L +RIN++ A++ + +G V +L E +G T VN +
Sbjct: 359 KQRLYILQDRINQQTTAWSRRKLGTVQRILVEGTSRKNVMELSGRTECNRVVNFEGSPEH 418
Query: 412 SGKILNVKI-----NSFEGEVAEGE 431
GK ++V+I NS G + GE
Sbjct: 419 IGKFVDVEITDVYANSLRGMLLRGE 443
>MIAB_COXBU RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 439
Score = 182 bits (463), Expect = 4e-48
Identities = 137/441 (31%), Positives = 210/441 (47%), Gaps = 15/441 (3%)
Query: 2 KVAFATLGCRVNTYESEAMTE--KFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQI 59
K+ T GC++N Y+S M + KF G E+ + +ADV+++NTCSV K
Sbjct: 3 KLYLKTHGCQMNEYDSAKMADVLKF-SHGLELTEDPAVADVFLLNTCSVREKAQTKVFSE 61
Query: 60 IGRAR---RQNPEAIIAVVGCYA-QIAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEG 115
+GR R + P +I V GC A Q T + VD+V G + + ++ +
Sbjct: 62 LGRWRPFKEKRPHVVIGVGGCVASQEGETILKQAPFVDIVFGPQTLHRLPDLLDSVIQKR 121
Query: 116 KQQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKK 175
K V + K F+ L + AF+ I +GC+++C +C++PYTRG S+
Sbjct: 122 KSVVDITFPEIEK-FDRLPQPRAEGPS-AFVSIMEGCSKYCTFCVVPYTRGEEISRPFDD 179
Query: 176 VLDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKVT-LVDLLEEIEKIDGIERVRIGSID 233
V+ E+ SL E G +EI L G + Y G+ D +V L L+ + +D IER+R +
Sbjct: 180 VIAEVASLCEQGVREITLLGQNVNDYRGLMHDGQVADLALLIHYLAAMDNIERIRFTTSH 239
Query: 234 PTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDV 293
P+ F+E+++ KL H HL +QSG D L M R YT EY+ + LR D+
Sbjct: 240 PSAFSENLIDAYAEEPKLANHLHLPVQSGSDRILAAMKRNYTVLEYKSKIRKLRAVRPDI 299
Query: 294 SITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDE 353
S+++D I+GFPG + + + F YSPR GT A + + V K E
Sbjct: 300 SLSSDFIIGFPGETDADFEATMNLIHDMGFDHSFSFIYSPRPGTPAAQLPDDVPMAVKKE 359
Query: 354 RSKKLIELNRINEKA--FAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNV 411
R I NRIN KA ++ +G +L + +G T N VN D +
Sbjct: 360 R--LAILQNRINAKAAEISQSMVGTQQRILVTGPSKKYPDQLSGRTENNRVVNFNGDTPL 417
Query: 412 SGKILNVKINSFEGEVAEGEI 432
G+++ +KI GEI
Sbjct: 418 IGQMVTIKIKEARPYSLWGEI 438
>MIAB_COXBR RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 439
Score = 182 bits (463), Expect = 4e-48
Identities = 137/441 (31%), Positives = 210/441 (47%), Gaps = 15/441 (3%)
Query: 2 KVAFATLGCRVNTYESEAMTE--KFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQI 59
K+ T GC++N Y+S M + KF G E+ + +ADV+++NTCSV K
Sbjct: 3 KLYLKTHGCQMNEYDSAKMADVLKF-SHGLELTEDPAVADVFLLNTCSVREKAQTKVFSE 61
Query: 60 IGRAR---RQNPEAIIAVVGCYA-QIAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEG 115
+GR R + P +I V GC A Q T + VD+V G + + ++ +
Sbjct: 62 LGRWRPFKEKRPHVVIGVGGCVASQEGETILKQAPFVDIVFGPQTLHRLPDLLDSVIQKR 121
Query: 116 KQQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKK 175
K V + K F+ L + AF+ I +GC+++C +C++PYTRG S+
Sbjct: 122 KSVVDITFPEIEK-FDRLPQPRAEGPS-AFVSIMEGCSKYCTFCVVPYTRGEEISRPFDD 179
Query: 176 VLDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKVT-LVDLLEEIEKIDGIERVRIGSID 233
V+ E+ SL E G +EI L G + Y G+ D +V L L+ + +D IER+R +
Sbjct: 180 VIAEVASLCEQGVREITLLGQNVNDYRGLMHDGQVADLALLIHYLAAMDNIERIRFTTSH 239
Query: 234 PTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDV 293
P+ F+E+++ KL H HL +QSG D L M R YT EY+ + LR D+
Sbjct: 240 PSAFSENLIDAYAEEPKLANHLHLPVQSGSDRILAAMKRNYTVLEYKSKIRKLRAVRPDI 299
Query: 294 SITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDE 353
S+++D I+GFPG + + + F YSPR GT A + + V K E
Sbjct: 300 SLSSDFIIGFPGETDADFEATMNLIHDMGFDHSFSFIYSPRPGTPAAQLPDDVPMAVKKE 359
Query: 354 RSKKLIELNRINEKA--FAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNV 411
R I NRIN KA ++ +G +L + +G T N VN D +
Sbjct: 360 R--LAILQNRINAKAAEISQSMVGTQQRILVTGPSKKYPDQLSGRTENNRVVNFNGDTPL 417
Query: 412 SGKILNVKINSFEGEVAEGEI 432
G+++ +KI GEI
Sbjct: 418 IGQMVTIKIKEARPYSLWGEI 438
>MIAB_COXB2 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 439
Score = 182 bits (463), Expect = 4e-48
Identities = 137/441 (31%), Positives = 210/441 (47%), Gaps = 15/441 (3%)
Query: 2 KVAFATLGCRVNTYESEAMTE--KFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQI 59
K+ T GC++N Y+S M + KF G E+ + +ADV+++NTCSV K
Sbjct: 3 KLYLKTHGCQMNEYDSAKMADVLKF-SHGLELTEDPAVADVFLLNTCSVREKAQTKVFSE 61
Query: 60 IGRAR---RQNPEAIIAVVGCYA-QIAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEG 115
+GR R + P +I V GC A Q T + VD+V G + + ++ +
Sbjct: 62 LGRWRPFKEKRPHVVIGVGGCVASQEGETILKQAPFVDIVFGPQTLHRLPDLLDSVIQKR 121
Query: 116 KQQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKK 175
K V + K F+ L + AF+ I +GC+++C +C++PYTRG S+
Sbjct: 122 KSVVDITFPEIEK-FDRLPQPRAEGPS-AFVSIMEGCSKYCTFCVVPYTRGEEISRPFDD 179
Query: 176 VLDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKVT-LVDLLEEIEKIDGIERVRIGSID 233
V+ E+ SL E G +EI L G + Y G+ D +V L L+ + +D IER+R +
Sbjct: 180 VIAEVASLCEQGVREITLLGQNVNDYRGLMHDGQVADLALLIHYLAAMDNIERIRFTTSH 239
Query: 234 PTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDV 293
P+ F+E+++ KL H HL +QSG D L M R YT EY+ + LR D+
Sbjct: 240 PSAFSENLIDAYAEEPKLANHLHLPVQSGSDRILAAMKRNYTVLEYKSKIRKLRAVRPDI 299
Query: 294 SITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDE 353
S+++D I+GFPG + + + F YSPR GT A + + V K E
Sbjct: 300 SLSSDFIIGFPGETDADFEATMNLIHDMGFDHSFSFIYSPRPGTPAAQLPDDVPMAVKKE 359
Query: 354 RSKKLIELNRINEKA--FAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNV 411
R I NRIN KA ++ +G +L + +G T N VN D +
Sbjct: 360 R--LAILQNRINAKAAEISQSMVGTQQRILVTGPSKKYPDQLSGRTENNRVVNFNGDTPL 417
Query: 412 SGKILNVKINSFEGEVAEGEI 432
G+++ +KI GEI
Sbjct: 418 IGQMVTIKIKEARPYSLWGEI 438
>MIAB_COXB1 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 439
Score = 182 bits (463), Expect = 4e-48
Identities = 137/441 (31%), Positives = 210/441 (47%), Gaps = 15/441 (3%)
Query: 2 KVAFATLGCRVNTYESEAMTE--KFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQI 59
K+ T GC++N Y+S M + KF G E+ + +ADV+++NTCSV K
Sbjct: 3 KLYLKTHGCQMNEYDSAKMADVLKF-SHGLELTEDPAVADVFLLNTCSVREKAQTKVFSE 61
Query: 60 IGRAR---RQNPEAIIAVVGCYA-QIAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEG 115
+GR R + P +I V GC A Q T + VD+V G + + ++ +
Sbjct: 62 LGRWRPFKEKRPHVVIGVGGCVASQEGETILKQAPFVDIVFGPQTLHRLPDLLDSVIQKR 121
Query: 116 KQQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKK 175
K V + K F+ L + AF+ I +GC+++C +C++PYTRG S+
Sbjct: 122 KSVVDITFPEIEK-FDRLPQPRAEGPS-AFVSIMEGCSKYCTFCVVPYTRGEEISRPFDD 179
Query: 176 VLDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKVT-LVDLLEEIEKIDGIERVRIGSID 233
V+ E+ SL E G +EI L G + Y G+ D +V L L+ + +D IER+R +
Sbjct: 180 VIAEVASLCEQGVREITLLGQNVNDYRGLMHDGQVADLALLIHYLAAMDNIERIRFTTSH 239
Query: 234 PTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDV 293
P+ F+E+++ KL H HL +QSG D L M R YT EY+ + LR D+
Sbjct: 240 PSAFSENLIDAYAEEPKLANHLHLPVQSGSDRILAAMKRNYTVLEYKSKIRKLRAVRPDI 299
Query: 294 SITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDE 353
S+++D I+GFPG + + + F YSPR GT A + + V K E
Sbjct: 300 SLSSDFIIGFPGETDADFEATMNLIHDMGFDHSFSFIYSPRPGTPAAQLPDDVPMAVKKE 359
Query: 354 RSKKLIELNRINEKA--FAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNV 411
R I NRIN KA ++ +G +L + +G T N VN D +
Sbjct: 360 R--LAILQNRINAKAAEISQSMVGTQQRILVTGPSKKYPDQLSGRTENNRVVNFNGDTPL 417
Query: 412 SGKILNVKINSFEGEVAEGEI 432
G+++ +KI GEI
Sbjct: 418 IGQMVTIKIKEARPYSLWGEI 438
>MIAB_AERHH RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 477
Score = 182 bits (463), Expect = 4e-48
Identities = 130/442 (29%), Positives = 207/442 (46%), Gaps = 14/442 (3%)
Query: 2 KVAFATLGCRVNTYESEAMTEKF-VREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S M + GY + + E ADV ++NTCS+ +K +
Sbjct: 4 KLHIKTWGCQMNEYDSSKMADLLDASNGYTLTEEPEEADVLLLNTCSIREKAQEKVFHQL 63
Query: 61 GRARR---QNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR ++ P +I V GC A + + VD+V G + + + + ++
Sbjct: 64 GRWKKLKANKPGLVIGVGGCVASQEGDNIRTRAPYVDIVFGPQTLHRLPTMIKQVQEGRG 123
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
QV V A + + F+ L T AF+ I +GC+++C++C++PYTRG S+ V
Sbjct: 124 AQVDV-AFPEIEKFDSLPEPRAEGAT-AFVSIMEGCSKYCSFCVVPYTRGEEVSRPLDDV 181
Query: 177 LDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKV-TLVDLLEEIEKIDGIERVRIGSIDP 234
L EI LA+ G +E+ L G + +Y G D + T +LL + IDGI+R+R + P
Sbjct: 182 LYEIAQLAQQGVREVNLLGQNVNAYRGPTFDGGICTFAELLRLVAAIDGIDRIRYTTSHP 241
Query: 235 TFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVS 294
FT+D++ ++ HL +QSG D L M R +T EY+ + LR D++
Sbjct: 242 IEFTDDIIEVYKDTPEVVSFLHLPVQSGSDRILTMMKRPHTVLEYKSKIRRLRAARPDIT 301
Query: 295 ITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDER 354
I++D IVGFP ++ I + F YSPR GT A ++ + VD K R
Sbjct: 302 ISSDFIVGFPNETDEDFEATMKLIEEINFDMSFSFIYSPRPGTPAADLPDDVDMEVKKAR 361
Query: 355 SKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSG- 413
+L + +G +L E +L G T N VN + + G
Sbjct: 362 LTRLQHVINNQSMQIGRAMLGSTQRILVEGPSKLDPMQLCGRTENNRVVNFEGPHTLIGG 421
Query: 414 ----KILNVKINSFEGEVAEGE 431
+I V+ NS G+ GE
Sbjct: 422 FADVEITEVRPNSLRGKFLRGE 443
>MIAB_PSEPF RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 442
Score = 182 bits (462), Expect = 5e-48
Identities = 130/445 (29%), Positives = 219/445 (49%), Gaps = 20/445 (4%)
Query: 2 KVAFATLGCRVNTYESEAMTEKF-VREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S M + + EV E ADV ++NTCS+ + +
Sbjct: 4 KLYIETHGCQMNEYDSSRMVDLLGEHQALEVTARAEDADVILLNTCSIRERAQDRVYSQL 63
Query: 61 GRARR---QNPEAIIAVVGCYAQIAPTEVSGIEG----VDVVLGSRNKGEIVYYVNKARD 113
GR R NP+ +IAV GC +A E + I VDVV G + + ++ AR
Sbjct: 64 GRWRELKLANPDMVIAVGGC---VASQEGAAIRDRAPYVDVVFGPQTLHRLPEMIDAARI 120
Query: 114 EGKQQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDP 173
QV V K F+ L + + A++ + +GC+++C +C++PYTRG S+
Sbjct: 121 SKLPQVDVSFPEIEK-FDHLPEPRIDGPS-AYVSVMEGCSKYCTFCVVPYTRGEEVSRPF 178
Query: 174 KKVLDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKVT-LVDLLEEIEKIDGIERVRIGS 231
V+ EI LAE+G +E+ L G + Y G+ D ++ L +L+ + +DGI+R+R +
Sbjct: 179 DDVIAEIIHLAENGVREVTLLGQNVNGYRGLTHDGRLADLAELIRVVAAVDGIDRIRYTT 238
Query: 232 IDPTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIE 291
P F++ +++ + +L H HL +QSG D L M R +TA EY+ + LR +
Sbjct: 239 SHPLEFSDSLIQAHAEVPELVKHLHLPVQSGSDRILAAMKRNHTALEYKSKLRKLRAAVP 298
Query: 292 DVSITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKK 351
+ I++D IVGFPG + + ++ F YS R GT A ++ + K
Sbjct: 299 GICISSDFIVGFPGETEKDFEQTMKLIADVGFDFSYSFVYSQRPGTPAADLADDTPEELK 358
Query: 352 DERSKKLIELNRINEKAF--AEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKAD- 408
ER L +R+N++ F + + +G V +L + + G G T N VN + D
Sbjct: 359 KERLNAL--QHRLNQQGFEISRQMVGSVQRILVTDYSKKDPGELQGRTENNRIVNFRCDN 416
Query: 409 CNVSGKILNVKINSFEGEVAEGEIV 433
+ G+ +V I++ + G ++
Sbjct: 417 PTLIGQFADVHIDAAQPHSLRGSLI 441
>MIAB_LEIXX RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 535
Score = 182 bits (462), Expect = 5e-48
Identities = 115/379 (30%), Positives = 181/379 (47%), Gaps = 7/379 (1%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIGR---A 63
T GC++N ++SE ++ GY + E AD+ V+NTC+V D K +G
Sbjct: 30 TFGCQMNVHDSERLSGSLEAAGYVPAN-GEEADIVVINTCAVRENADNKLYGNLGHLASV 88
Query: 64 RRQNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVKVG 122
+R++ IAV GC AQ + VDVV G+ N G + + +AR
Sbjct: 89 KRRHAGMQIAVGGCLAQKDKNVILEKAPWVDVVFGTHNMGALPRLLERARHN--DXXXXX 146
Query: 123 AVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDEIRS 182
F + ++ ++ I GCN C +C++P RG + P +L E+++
Sbjct: 147 XXXXXXTFPSTLPTKRDSSFSGWVSISVGCNNTCTFCIVPALRGKEKDRRPGDILAEVQA 206
Query: 183 LAEHGFKEIILSGIHTASYGVDLDEKVTLVDLLEEIEKIDGIERVRIGSIDPTFFTEDVV 242
L + G E+ L G + SYGV+ ++ LL +I+G+ER+R S P FTEDV+
Sbjct: 207 LVDEGAVEVTLLGQNVNSYGVEFGDRQAFSKLLRAAGQIEGLERIRFTSPHPAAFTEDVI 266
Query: 243 RRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITTDVIVG 302
+ + P H+ LQSG D L+ M R Y ++++ I++ +R K+ +I+TD+IVG
Sbjct: 267 DAMAETPNVMPQLHMPLQSGSDRILRSMRRSYRSEKFLGILDRVRAKLPGAAISTDIIVG 326
Query: 303 FPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSKKLIELN 362
FPG ++ + F+YS R GT A M QV ER +LI L
Sbjct: 327 FPGETEEDFLDTLRVVEAARFASAFTFQYSIRPGTPAATMAGQVPKEVVQERYDRLIALQ 386
Query: 363 RINEKAFAEKYIGEVIDVL 381
EK IG +++L
Sbjct: 387 ERISLEENEKLIGRDVELL 405
>MIAB_HELPG RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 437
Score = 182 bits (462), Expect = 5e-48
Identities = 132/434 (30%), Positives = 214/434 (49%), Gaps = 22/434 (5%)
Query: 1 MKVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
MKV T+GC +N+ +SE + + + Y+ + AD+ ++NTCSV ++K I
Sbjct: 1 MKVYIETMGCAMNSRDSEHLLSELSKLDYKETSDPKTADLILINTCSVREKPERKLFSEI 60
Query: 61 GR-ARRQNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQ 118
G+ A+ + P A I V GC A ++ V VLG+RN +I ++K ++
Sbjct: 61 GQFAKIKKPNAKIGVCGCTASHMGADILKKAPSVSFVLGARNVSKISQVIHK-----EKA 115
Query: 119 VKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLD 178
V+V + + E + R+ L I GC++ CAYC++P+TRG S +L
Sbjct: 116 VEVAIDYDESAYA-FEFFEKKAQIRSLLNISIGCDKKCAYCIVPHTRGKEISIPMDLILK 174
Query: 179 EIRSLAEHGFKEIILSGIHTASYGVDLDE---KVTLVDLLEEIEKIDGIERVRIGSIDPT 235
E LA +G KE++L G + +YG KV DLL+++ +I GIER+R S P
Sbjct: 175 EAEKLANNGTKELMLLGQNVNNYGARFSSEHAKVDFSDLLDKLSEISGIERIRFTSPHPL 234
Query: 236 FFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSI 295
+ + R K+C H+ LQSG A LK M R Y+ + + + V L+ + +V I
Sbjct: 235 HMNDGFLERFAKNPKVCKSIHMPLQSGSSAVLKMMRRGYSKEWFLNRVERLKALVPEVGI 294
Query: 296 TTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERS 355
+TD+IVGFP L+++ + F YSPR T A + +V R
Sbjct: 295 STDIIVGFPNESDKDFEDTMEVLEKVRFDTLYSFIYSPRPFTEAGAWKERVPLEVSSSRL 354
Query: 356 KKLIELNR--INEKAFAEKYIGEVIDVLFEEEVELGSGVYTGY-----TRNYIKVNAKAD 408
++L ++ + EKA E +G+ VL E E+ + + G+ T +I+V K
Sbjct: 355 ERLQNRHKEILEEKAKLE--VGKTHVVLVENRREINNQI-VGFEGRSDTGKFIEVACKEK 411
Query: 409 CNVSGKILNVKINS 422
N G+++ V+I S
Sbjct: 412 RN-PGELVEVEIIS 424
>MIAB_COXBN RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 439
Score = 182 bits (462), Expect = 5e-48
Identities = 137/441 (31%), Positives = 210/441 (47%), Gaps = 15/441 (3%)
Query: 2 KVAFATLGCRVNTYESEAMTE--KFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQI 59
K+ T GC++N Y+S M + KF G E+ + +ADV+++NTCSV K
Sbjct: 3 KLYLKTHGCQMNEYDSAKMADVLKF-SHGLELTEDPAVADVFLLNTCSVREKAQTKVFSE 61
Query: 60 IGRAR---RQNPEAIIAVVGCYA-QIAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEG 115
+GR R + P +I V GC A Q T + VD+V G + + ++ +
Sbjct: 62 LGRWRPFKEKRPHVVIGVGGCVASQEGETILKQAPFVDIVFGPQTLHRLPDLLDSVIQKR 121
Query: 116 KQQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKK 175
K V + K F+ L + AF+ I +GC+++C +C++PYTRG S+
Sbjct: 122 KSVVDITFPEIEK-FDRLPQPRAEGPS-AFVSIMEGCSKYCTFCVVPYTRGEEISRPFDD 179
Query: 176 VLDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKVT-LVDLLEEIEKIDGIERVRIGSID 233
V+ E+ SL E G +EI L G + Y G+ D +V L L+ + +D IER+R +
Sbjct: 180 VIAEVASLCEQGVREITLLGQNVNDYCGLMHDGQVADLALLIHYLAAMDNIERIRFTTSH 239
Query: 234 PTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDV 293
P+ F+E+++ KL H HL +QSG D L M R YT EY+ + LR D+
Sbjct: 240 PSAFSENLIDAYAEEPKLANHLHLPVQSGSDRILAAMKRNYTVLEYKSKIRKLRAVRPDI 299
Query: 294 SITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDE 353
S+++D I+GFPG + + + F YSPR GT A + + V K E
Sbjct: 300 SLSSDFIIGFPGETDADFEATMNLIHDMGFDHSFSFIYSPRPGTPAAQLPDDVPMAVKKE 359
Query: 354 RSKKLIELNRINEKA--FAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNV 411
R I NRIN KA ++ +G +L + +G T N VN D +
Sbjct: 360 R--LAILQNRINVKAAEISQSMVGTQQRILVTGPSKKYPDQLSGRTENNRVVNFNGDTPL 417
Query: 412 SGKILNVKINSFEGEVAEGEI 432
G+++ +KI GEI
Sbjct: 418 IGQMVTIKIKEARPYSLWGEI 438
>MIAB_BORPA RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 475
Score = 182 bits (461), Expect = 6e-48
Identities = 134/440 (30%), Positives = 202/440 (45%), Gaps = 15/440 (3%)
Query: 2 KVAFATLGCRVNTYESEAMTEKF-VREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S+ M + +G E+ D E ADV + NTCSV +K +
Sbjct: 28 KLYIRTFGCQMNEYDSDKMADVLRADQGLELTDNPEDADVILFNTCSVREKAQEKVFSDL 87
Query: 61 GRA---RRQNPEAIIAVVGCYA-QIAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR ++QNP +I V GC A Q V VDVV G + + + + R +G
Sbjct: 88 GRVQHLKKQNPNLVIGVGGCVASQEGEAIVKRAPYVDVVFGPQTLHRLPDLIKRRRAQGV 147
Query: 117 QQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKV 176
QV + K F+ L + T AF+ I +GC+++C++C++PYTRG S+ V
Sbjct: 148 SQVDISFPEIEK-FDALPPPRVDGAT-AFVSIMEGCSKYCSFCVVPYTRGEEVSRPFDDV 205
Query: 177 LDEIRSLAEHGFKEIILSGIHTASYGVDLDEKVTLVD---LLEEIEKIDGIERVRIGSID 233
L EI LA+ G KE+ L G + +Y + + + D LLE + +I GIER+R +
Sbjct: 206 LLEIADLADQGVKEVTLLGQNVNAYRGAMGDSGEIADFAMLLEYVHEIPGIERIRYTTSH 265
Query: 234 PTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDV 293
P T+ +V L KL HL +Q+G D L M R YTA E++ +V LR +
Sbjct: 266 PKEMTQRMVDAYARLPKLVSFLHLPVQAGSDRVLAAMKRGYTALEFKSVVRRLRAARPSL 325
Query: 294 SITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDE 353
++++D IVGFPG ++ + + F YS R GT A ++ + + K
Sbjct: 326 TLSSDFIVGFPGETEEDFQKTMKLIEDVGFDTSFSFVYSRRPGTPAADLHDDTPQDVKXX 385
Query: 354 RSKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSG 413
IG VL E G T N VN + G
Sbjct: 386 XXXXXXXXXXXXXXXXXXGMIGTRQRVLVEGPSRRDPNELMGRTENNRIVNFPGVPRLIG 445
Query: 414 KILNVKI-----NSFEGEVA 428
+++V + NS G VA
Sbjct: 446 HMVDVVVTHAHTNSLRGRVA 465
>MIAB_SYNS3 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 473
Score = 181 bits (460), Expect = 8e-48
Identities = 115/388 (29%), Positives = 182/388 (46%), Gaps = 15/388 (3%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIGRA--- 63
T GC++N +SE M GY+ + AD+ + NTC++ + ++K +GR
Sbjct: 33 TFGCQMNKADSERMAGILETMGYQEANAELDADLVLYNTCTIRDNAEQKVYSYLGRQAIR 92
Query: 64 RRQNPEAIIAVVGCYAQI-APTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVKVG 122
+R NP + V GC AQ + + + +D+V+G ++ +
Sbjct: 93 KRTNPNLTLVVAGCVAQQEGESLLRRVPELDLVMGPQHANRL----EXXXXXXXXXXXXX 148
Query: 123 AVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDEIRS 182
+ + EDL ++ T A++ + GCN C YC++P RG S+ P+ +L E+
Sbjct: 149 XXEDHHILEDLTTARRDSSTCAWVNVIYGCNERCTYCVVPSVRGKEQSRLPESILLEMEG 208
Query: 183 LAEHGFKEIILSGIHTASYGVDLD-------EKVTLVDLLEEIEKIDGIERVRIGSIDPT 235
LA GFKEI L G + +YG DL + TL DLL ++ ++GIER+R + P
Sbjct: 209 LAARGFKEITLLGQNIDAYGRDLPGITAEGRRQHTLTDLLHQVHDVEGIERLRFATSHPR 268
Query: 236 FFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSI 295
+FTE ++ L K+C HFH+ QSG + LK M R YT + + D +I
Sbjct: 269 YFTERLIDACADLPKVCEHFHIPFQSGDNELLKAMARGYTVEXXXXXXXXXXXXMPDAAI 328
Query: 296 TTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERS 355
+ DVIV FPG ++ I + + YSPR T A + NQ+ K R
Sbjct: 329 SADVIVAFPGETDAQYRRTLALIEEIGFDQVNTAAYSPRPNTPAADWNNQLSEEVKVARL 388
Query: 356 KKLIELNRINEKAFAEKYIGEVIDVLFE 383
+++ L K +Y G + VL E
Sbjct: 389 QEINALVESTAKERNARYAGRIEQVLAE 416
>MIAB_AZOSB RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 449
Score = 181 bits (460), Expect = 8e-48
Identities = 139/449 (30%), Positives = 210/449 (46%), Gaps = 23/449 (5%)
Query: 2 KVAFATLGCRVNTYESEAMTEKF-VREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+SE M + EG D E ADV + NTCSV +K +
Sbjct: 3 KLYIRTFGCQMNEYDSEKMADVLGAGEGIAKTDNPEEADVILFNTCSVREKAQEKVFHDL 62
Query: 61 GRAR---RQNPEAIIAVVGCYA-QIAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGK 116
GR + + P+ II V GC A Q V+ VDVV G + + + + G+
Sbjct: 63 GRVKHLKQLKPDLIIGVGGCVASQEGDAIVARAPYVDVVFGPQTLHRLPALIAARKQSGR 122
Query: 117 QQVKVG--AVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPK 174
QV + + K ++E + AF+ I +GC+++C +C++PYTRG S+ +
Sbjct: 123 SQVDISFPEIEKFDAMPPARVEGAS----AFVSIMEGCSKYCTFCVVPYTRGEEISRPLE 178
Query: 175 KVLDEIRSLAEHGFKEIILSGIHTASYGV----DLDEKVTLVDLLEEIEKIDGIERVRIG 230
VL EI LAE G KE+ L G + ++ D E+ LLE + +I GIER+R
Sbjct: 179 DVLAEIAGLAEQGVKEVTLLGQNVNAWRGEIVRDAGEEGDFAFLLECVAEIPGIERIRYT 238
Query: 231 SIDPTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKI 290
+ P T+ + + + KL HL +QSG D L M R Y+ E++ IV LR
Sbjct: 239 TSHPREMTQRLFDAYVKIPKLVSQLHLPVQSGSDRILAAMKRGYSVLEFKSIVRKLRAAR 298
Query: 291 EDVSITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNK 350
D+S+++D IVGFPG + + + F YS R GT A ++E+ V
Sbjct: 299 PDLSLSSDFIVGFPGETEEDFEKTMKLIDEVGFDGSFSFVYSARPGTPAADLEDPVPQET 358
Query: 351 KDERSKKLIELNRINEKAFA--EKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKAD 408
K +L RI+E+A A + +G +L E + G T N VN A
Sbjct: 359 KLAWLARL--QKRIDEQAQAVSQSMVGSAQRILVEGVSRKSAEELMGRTDNNRVVNFPAP 416
Query: 409 C----NVSGKILNVKINSFEGEVAEGEIV 433
+ G+ + V+I S GEI+
Sbjct: 417 SPHRDRLVGQFIEVRITSALPHSLRGEIL 445
>RIMO_CLOPE RecName: Full=Ribosomal protein S12
methylthiotransferase rimO; Short=S12
methylthiotransferase; Short=S12 MTTase;
Length = 445
Score = 181 bits (459), Expect = 1e-47
Identities = 114/387 (29%), Positives = 197/387 (50%), Gaps = 6/387 (1%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII- 60
KV +LGC N +SE M V+ YE+ + + AD+ +VNTC ++S I
Sbjct: 5 KVGMVSLGCDKNRVDSEIMLG-MVQNEYELTNNPKEADIIIVNTCGFIEKAKQESINTIL 63
Query: 61 --GRARRQNPEAIIAVVGCYAQIAPTEVSGI-EGVDVVLGSRNKGEIVYYVNKARDEGKQ 117
+ + + ++ GC Q E+ + +D++LG + +I + + +
Sbjct: 64 DMAKYKTSHNCKLLIATGCLTQRYGDELLELMPEIDIMLGVNDYAKINEAIMNFINGNNE 123
Query: 118 QVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVL 177
+VK + E L++ +K A+L+I +GC+ FC YC+IP RG S+ + ++
Sbjct: 124 KVKATNYSDVSINEGLRLIT-TDKATAYLRIAEGCDNFCTYCIIPKIRGKFRSRALESIV 182
Query: 178 DEIRSLAEHGFKEIILSGIHTASYGVDLDEKVTLVDLLEEIEKIDGIERVRIGSIDPTFF 237
+E + LAE+G KE+IL T +YG+D+ + L +L E+ KI+GIE +R+ P
Sbjct: 183 EEAKKLAENGVKELILIAQDTTNYGIDIYGEKKLHLVLRELAKIEGIEWIRVLYCYPEAI 242
Query: 238 TEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITT 297
+++++ I K+C + L +Q + LKRM R+ T +E +N LR + ++ + T
Sbjct: 243 YDELIKEISVNDKVCNYLDLPIQHISNNVLKRMGRKTTKEEIIGKINDLRKNVPNIVLRT 302
Query: 298 DVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSKK 357
+IVGFPG +K I L K +F YS +GT A ME+Q+D K R ++
Sbjct: 303 SLIVGFPGESCEDFNELKDFIKTIKLDKVGVFTYSREEGTPAAIMEDQIDEEVKKAREEE 362
Query: 358 LIELNRINEKAFAEKYIGEVIDVLFEE 384
++ L + + + +G DVL E+
Sbjct: 363 IMLLQKEVSEEINKNKVGREYDVLIEK 389
>RIMO_CLOP1 RecName: Full=Ribosomal protein S12
methylthiotransferase rimO; Short=S12
methylthiotransferase; Short=S12 MTTase;
Length = 445
Score = 181 bits (459), Expect = 1e-47
Identities = 114/387 (29%), Positives = 197/387 (50%), Gaps = 6/387 (1%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII- 60
KV +LGC N +SE M V+ YE+ + + AD+ +VNTC ++S I
Sbjct: 5 KVGMVSLGCDKNRVDSEIMLG-MVQNEYELTNNPKEADIIIVNTCGFIEKAKQESINTIL 63
Query: 61 --GRARRQNPEAIIAVVGCYAQIAPTEVSGI-EGVDVVLGSRNKGEIVYYVNKARDEGKQ 117
+ + + ++ GC Q E+ + +D++LG + +I + + +
Sbjct: 64 DMAKYKTSHNCKLLIATGCLTQRYGDELLELMPEIDIMLGVNDYAKINEAIMNFINGNNE 123
Query: 118 QVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVL 177
+VK + E L++ +K A+L+I +GC+ FC YC+IP RG S+ + ++
Sbjct: 124 KVKATNYSDVSINEGLRLIT-TDKATAYLRIAEGCDNFCTYCIIPKIRGKFRSRALESIV 182
Query: 178 DEIRSLAEHGFKEIILSGIHTASYGVDLDEKVTLVDLLEEIEKIDGIERVRIGSIDPTFF 237
+E + LAE+G KE+IL T +YG+D+ + L +L E+ KI+GIE +R+ P
Sbjct: 183 EEAKKLAENGVKELILIAQDTTNYGIDIYGEKKLHLVLRELAKIEGIEWIRVLYCYPEAI 242
Query: 238 TEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITT 297
+++++ I K+C + L +Q + LKRM R+ T +E +N LR + ++ + T
Sbjct: 243 YDELIKEISVNDKVCNYLDLPIQHISNNVLKRMGRKTTKEEIIGKINDLRKNVPNIVLRT 302
Query: 298 DVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSKK 357
+IVGFPG +K I L K +F YS +GT A ME+Q+D K R ++
Sbjct: 303 SLIVGFPGESCEDFNELKDFIKTIKLDKVGVFTYSREEGTPAAIMEDQIDEEVKKAREEE 362
Query: 358 LIELNRINEKAFAEKYIGEVIDVLFEE 384
++ L + + + +G DVL E+
Sbjct: 363 IMLLQKEVSEEINKNKVGREYDVLIEK 389
>MIAB_PSEF5 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 442
Score = 181 bits (459), Expect = 1e-47
Identities = 132/445 (29%), Positives = 219/445 (49%), Gaps = 20/445 (4%)
Query: 2 KVAFATLGCRVNTYESEAMTEKF-VREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQII 60
K+ T GC++N Y+S M + + EV E ADV ++NTCS+ + +
Sbjct: 4 KLYIETHGCQMNEYDSSRMVDLLGEHQALEVTARAEDADVILLNTCSIRERAQDRVYSQL 63
Query: 61 GRARR---QNPEAIIAVVGCYAQIAPTEVSGIEG----VDVVLGSRNKGEIVYYVNKARD 113
GR R NP+ +IAV GC +A E + I VDVV G + + ++ AR
Sbjct: 64 GRWRELKLANPDMVIAVGGC---VASQEGAAIRDRAPYVDVVFGPQTLHRLPEMIDVARI 120
Query: 114 EGKQQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDP 173
QV V K F+ L + + A++ + +GC+++C +C++PYTRG S+
Sbjct: 121 TKLPQVDVSFPEIEK-FDHLPEPRIDGPS-AYVSVMEGCSKYCTFCVVPYTRGEEVSRPF 178
Query: 174 KKVLDEIRSLAEHGFKEIILSGIHTASY-GVDLDEKVT-LVDLLEEIEKIDGIERVRIGS 231
V+ EI LAE+G +E+ L G + Y G D ++ L +L+ + IDGIER+R +
Sbjct: 179 DDVIAEIIHLAENGVREVTLLGQNVNGYRGQTHDGRMADLAELIRVVAAIDGIERIRYTT 238
Query: 232 IDPTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIE 291
P F++ +++ + +L H HL +QSG D L M R +TA EY+ + LR +
Sbjct: 239 SHPLEFSDSLIQAHAEVPELVKHLHLPVQSGSDRILAAMKRNHTALEYKSKLRKLRAAVP 298
Query: 292 DVSITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKK 351
+ I++D IVGFPG ++ + ++ F YS R GT A ++ ++ K
Sbjct: 299 GICISSDFIVGFPGETEKDFQQTMKLIEDVGFDFSYSFVYSQRPGTPAADLADETPEAVK 358
Query: 352 DERSKKLIELNRINEKAF--AEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKAD- 408
ER L +R+N++ F + + +G +L + + G G T N VN + D
Sbjct: 359 KERLNAL--QHRLNQQGFEISRQMVGSTQRILVTDYSKKDPGELQGRTENNRIVNFRCDN 416
Query: 409 CNVSGKILNVKINSFEGEVAEGEIV 433
+ G+ +V I+S + G ++
Sbjct: 417 PTLIGQFADVYIDSAQPHSLRGSLL 441
>MIAB_NITOC RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 447
Score = 181 bits (459), Expect = 1e-47
Identities = 136/443 (30%), Positives = 211/443 (47%), Gaps = 16/443 (3%)
Query: 2 KVAFATLGCRVNTYESEAMTEKFVRE--GYEVVDFNEMADVYVVNTCSVTNMGDKKSRQI 59
K+ T GC++N Y+S M + +RE G E+ E A+V ++NTCSV +K
Sbjct: 4 KLYIKTHGCQMNEYDSSRMAD-VLRESHGLELTSDPEQAEVLLLNTCSVREKAQEKVFSQ 62
Query: 60 IGRARR---QNPEAIIAVVGCYA-QIAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEG 115
+GR R+ PE +I V GC A Q T VD+V G + + + +A+
Sbjct: 63 LGRWRQLKQSRPELVIGVGGCVASQEGETIRVRAPYVDLVFGPQTIHRLPAMLTQAKASS 122
Query: 116 KQQVKVGAVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKK 175
+ V + K F+ L T AF+ + +GC+++C++C++PYTRG S+
Sbjct: 123 QPVVDLSFPEIEK-FDKLPEPRAEGPT-AFVSVMEGCSKYCSFCVVPYTRGEEISRPFDD 180
Query: 176 VLDEIRSLAEHGFKEIILSGIHTASYGVDLDEKVT--LVDLLEEIEKIDGIERVRIGSID 233
V+ EI LAE G +EI L G + +Y + E T L L+ + I+GIER+R +
Sbjct: 181 VIAEIVGLAEQGVREITLLGQNVNAYRGSIGEGDTADLALLIHYVASIEGIERIRFTTSH 240
Query: 234 PTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDV 293
P F++ +++ + L H HL +QSG D L M R +T EY+ + LR D+
Sbjct: 241 PVEFSDSLIQAFAEVPALVSHLHLPVQSGSDRMLSLMKRGHTVLEYKAKLRKLRQVRPDI 300
Query: 294 SITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDE 353
SI++D IVG PG + + + F YSPR GT A ++ + K E
Sbjct: 301 SISSDFIVGLPGETEADFQATLALVDEVGFDHSFSFIYSPRPGTPAASLPDCTPIALKKE 360
Query: 354 RSKKLIELNRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSG 413
R L R E A + + V VL E + + G T N VN A ++ G
Sbjct: 361 RLAILQGRLRALEIAISRGMVDTVQQVLVERPSKKDPTMLAGRTANNRVVNFSASQDMVG 420
Query: 414 KILNVKI-----NSFEGEVAEGE 431
+ +N++I NS G V + +
Sbjct: 421 QFVNIRITEALPNSLRGIVLDAK 443
>MIAB_MYCSS RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 525
Score = 181 bits (459), Expect = 1e-47
Identities = 122/396 (30%), Positives = 181/396 (45%), Gaps = 23/396 (5%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKK---SRQIIGRA 63
T GC++N ++SE + V V NTC+V D K + +
Sbjct: 20 TYGCQMNVHDSERLAGLLEXXXXXXXXXXXXXXVVVFNTCAVRENADNKLYGNLSHLAPR 79
Query: 64 RRQNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVKVG 122
+R P+ IAV GC AQ V VDVV G+ N G + + +AR QV++
Sbjct: 80 KRSEPQMQIAVGGCLAQKDRDAVLRRAPWVDVVFGTHNIGSLPTLLERARHNRAAQVEIA 139
Query: 123 AVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDEIRS 182
L+ F + ++ I GCN C +C++P RG + P VL EI++
Sbjct: 140 EALQE--FPSTLPAARESAYAGWVSISVGCNNTCTFCIVPSLRGKEVDRRPGDVLAEIQT 197
Query: 183 LAEHGFKEIILSGIHTASYGVDL--DEKV---------------TLVDLLEEIEKIDGIE 225
L + G E+ L G + +YGV DE++ +LL +IDG+E
Sbjct: 198 LVDQGVLEVTLLGQNVNAYGVSFAADERLREDPRMWQSVPRNRGAFAELLRACGRIDGLE 257
Query: 226 RVRIGSIDPTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNL 285
RVR S P FT+DV+ + +CP H+ LQSG D L+ M R Y A++Y I++
Sbjct: 258 RVRFTSPHPAEFTDDVIEAMAETPNVCPALHMPLQSGSDRILRAMRRSYRAEKYLGIIDR 317
Query: 286 LRDKIEDVSITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQ 345
+R I D +ITTD+IVGFPG + S F+YS R GT A +M Q
Sbjct: 318 VRAAIPDAAITTDLIVGFPGETEEDFQATLDVVAASRFSSAFTFQYSKRPGTPAADMPGQ 377
Query: 346 VDGNKKDERSKKLIELNRINEKAFAEKYIGEVIDVL 381
+ ER ++LIEL + +G +++L
Sbjct: 378 LPKAVVSERYQRLIELQERISLEENQAQVGRTLELL 413
>MIAB_MYCSK RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 529
Score = 181 bits (459), Expect = 1e-47
Identities = 122/396 (30%), Positives = 181/396 (45%), Gaps = 23/396 (5%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKK---SRQIIGRA 63
T GC++N ++SE + V V NTC+V D K + +
Sbjct: 24 TYGCQMNVHDSERLAGLLEXXXXXXXXXXXXXXVVVFNTCAVRENADNKLYGNLSHLAPR 83
Query: 64 RRQNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVKVG 122
+R P+ IAV GC AQ V VDVV G+ N G + + +AR QV++
Sbjct: 84 KRSEPQMQIAVGGCLAQKDRDAVLRRAPWVDVVFGTHNIGSLPTLLERARHNRAAQVEIA 143
Query: 123 AVLKNKVFEDLKIEEYNNKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDEIRS 182
L+ F + ++ I GCN C +C++P RG + P VL EI++
Sbjct: 144 EALQE--FPSTLPAARESAYAGWVSISVGCNNTCTFCIVPSLRGKEVDRRPGDVLAEIQT 201
Query: 183 LAEHGFKEIILSGIHTASYGVDL--DEKV---------------TLVDLLEEIEKIDGIE 225
L + G E+ L G + +YGV DE++ +LL +IDG+E
Sbjct: 202 LVDQGVLEVTLLGQNVNAYGVSFAADERLREDPRMWQSVPRNRGAFAELLRACGRIDGLE 261
Query: 226 RVRIGSIDPTFFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNL 285
RVR S P FT+DV+ + +CP H+ LQSG D L+ M R Y A++Y I++
Sbjct: 262 RVRFTSPHPAEFTDDVIEAMAETPNVCPALHMPLQSGSDRILRAMRRSYRAEKYLGIIDR 321
Query: 286 LRDKIEDVSITTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQ 345
+R I D +ITTD+IVGFPG + S F+YS R GT A +M Q
Sbjct: 322 VRAAIPDAAITTDLIVGFPGETEEDFQATLDVVAASRFSSAFTFQYSKRPGTPAADMPGQ 381
Query: 346 VDGNKKDERSKKLIELNRINEKAFAEKYIGEVIDVL 381
+ ER ++LIEL + +G +++L
Sbjct: 382 LPKAVVSERYQRLIELQERISLEENQAQVGRTLELL 417
>MIAB_HERA2 RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 438
Score = 181 bits (459), Expect = 1e-47
Identities = 129/429 (30%), Positives = 212/429 (49%), Gaps = 36/429 (8%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIG----- 61
T+GC++N +SE + GY + E AD V+N+CSV ++K IIG
Sbjct: 10 TVGCQMNVSDSERLESALQGVGYTPAEQAEDADFIVLNSCSVRANAEEK---IIGKITDI 66
Query: 62 -RARRQNPEAIIAVVGCYAQIAPTEVSGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVK 120
R +R+ P+ I + GC + P S + + + ++V+ + + +
Sbjct: 67 QRIKRERPDTKIVLWGC--MVGPNNQS--------IFKKKLPMVDHFVSPSAVD-----E 111
Query: 121 VGAVLKNKVFE----DLKIEEYN-NKTRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKK 175
V A+ N +++ L + ++ + I GCN CAYC+IP RG S+ ++
Sbjct: 112 VLALAPNPIYQLDEPALPVADWQVPPVNVHVPINYGCNMSCAYCVIPLRRGKERSRPMEE 171
Query: 176 VLDEIRSLAEHGFKEIILSGIHTASYGVDLDEKVTLVDLLEEIEKIDGIERVRIGSIDPT 235
+ +E+R + G KEI L G SYG DL + L DLLE + + G+ R+R + P
Sbjct: 172 IAEEVRRICARGAKEITLLGQIVDSYGHDLPGRPDLADLLEYLHETPGLVRLRFLTSHPA 231
Query: 236 FFTEDVVRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSI 295
F +E ++ I L K+ P +L +Q+G D LK M R YT +Y ++ +R+ I +VS+
Sbjct: 232 FMSEKLLHTIARLPKVMPDINLPIQAGDDQLLKVMKRGYTVAKYTKLIERIREIIPNVSL 291
Query: 296 TTDVIVGFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENME-NQVDGNKKDER 354
+TD+IVG PG ++ I K HI YS R GT+A +ME + + E+
Sbjct: 292 STDIIVGHPGETREMFERTLEMVENIRFDKVHIAAYSSRPGTKAADMELDPALAVEHGEK 351
Query: 355 SKKLIELNRINEKAFAEK---YIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNV 411
+ I L R+ E+ E+ +G ++VL E E G + G RN V +AD +
Sbjct: 352 QYRRIALERLQERIATERNEECLGHEVEVLVE---EFTKGKWRGRDRNNKLVFFEADGDW 408
Query: 412 SGKILNVKI 420
GK++N+ +
Sbjct: 409 YGKVVNIHV 417
>MIAB_GEOSL RecName: Full=(Dimethylallyl)adenosine tRNA
methylthiotransferase miaB; EC=2.-.-.-;AltName:
Full=tRNA-i(6)A37 methylthiotransferase;
Length = 446
Score = 181 bits (459), Expect = 1e-47
Identities = 126/432 (29%), Positives = 197/432 (45%), Gaps = 8/432 (1%)
Query: 7 TLGCRVNTYESEAMTEKFVREGYEVVDFNEMADVYVVNTCSVTNMGDKKSRQIIGR---A 63
T GC++N +SE + EGY D E AD+ ++NTCSV ++K +GR
Sbjct: 11 TFGCQMNVNDSEKIATLLKDEGYLPTDDPERADLVILNTCSVRAKAEQKVYGHLGRFKGV 70
Query: 64 RRQNPEAIIAVVGCYAQIAPTEV-SGIEGVDVVLGSRNKGEIVYYVNKARDEGKQQVKVG 122
R + ++ V GC AQ + + +D+V G+ N +
Sbjct: 71 RSRKKGFLLGVGGCVAQQEGERLLQKVPWLDLVFGTHNLHLLPEIXXXXXXXXXXXXXX- 129
Query: 123 AVLKNKVFEDLKIEEYNNK-TRAFLKIQDGCNRFCAYCLIPYTRGSVCSKDPKKVLDEIR 181
+ N+ DL E F+ + GC+ FC+YC++PY RG S+ ++DE+R
Sbjct: 130 -FIDNETRLDLFPETGGEGGVTRFVTVMQGCDNFCSYCIVPYVRGREISRRSSDIIDEVR 188
Query: 182 SLAEHGFKEIILSGIHTASYGVDLDEKVTLVDLLEEIEKIDGIERVRIGSIDPTFFTEDV 241
G KE+ L G + SYG+ + +++ L+ I +++G+ER+R + P + ++
Sbjct: 189 KSVAEGVKEVTLLGQNVNSYGLKTEGELSFAGLIRRIAEVEGLERIRFTTSHPKDISPEL 248
Query: 242 VRRILALKKLCPHFHLSLQSGCDATLKRMNRRYTAKEYEDIVNLLRDKIEDVSITTDVIV 301
+ + KLC H HL QSG DA L RMNR YT Y + V LR + T D+IV
Sbjct: 249 IACFAEVPKLCGHIHLPAQSGSDAVLARMNRGYTRAGYLEKVEALRAARPGIQFTGDMIV 308
Query: 302 GFPGXXXXXXXXXXXXLKRIALSKTHIFKYSPRKGTRAENMENQVDGNKKDERSKKLIEL 361
GFPG ++ + + F YSPR T A + + V +K R +L L
Sbjct: 309 GFPGETEEDFQATISLMEEVRYADLFSFIYSPRPETAAAGIRDTVSRKEKQSRLDRLQTL 368
Query: 362 NRINEKAFAEKYIGEVIDVLFEEEVELGSGVYTGYTRNYIKVNAKADCNVSGKILNV