RPS-BLAST 2.2.16 [Mar-25-2007]

Database: 95pdb09Oct16_0000; 95pdb09Oct16_0001; 95pdb09Oct16_0002;
95pdb09Oct16_0003 
           66,450 sequences; 15,983,541 total letters

Searching..................................................done

Query= ABE38048.1 rpal2 ABE38048.1 GIB00345CH01 "glutamate--cysteine
ligase"
         (456 letters)



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

2d32B [x.x.x] GLUTAMATE--CYSTEINE LIGASE                              213   1e-57
2d32D [x.x.x] GLUTAMATE--CYSTEINE LIGASE                              188   3e-50
2d33C [x.x.x] GLUTAMATE--CYSTEINE LIGASE                              178   4e-47
2d33B [x.x.x] GLUTAMATE--CYSTEINE LIGASE                              170   8e-45
2d32A [x.x.x] GLUTAMATE--CYSTEINE LIGASE                              146   1e-37

>2d32B [x.x.x] GLUTAMATE--CYSTEINE LIGASE
          Length = 499

 Score =  213 bits (542), Expect = 1e-57
 Identities = 54/449 (12%), Positives = 100/449 (22%), Gaps = 61/449 (13%)

Query: 43  TLEGHHPVPYAGARGIGALLEGMQLLLGWEPIMEGPHIIGLHDVTGGGAISLEPGGQFEL 102
            LE H        RG+      +    G       P  +G        +         + 
Sbjct: 11  WLEKHPQALKGIQRGLERETLRVNAD-GTLATTGHPEALG--------SALTHKWITTDF 61

Query: 103 SGAPLDNVHQTHAELMAHLAQ-------VREVATPLGIGFLGLGMTP----SWSREDIPV 151
           + A L+ +     ++   L                  +  L +                 
Sbjct: 62  AEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPLSMPSYIAEGQDIELAQYGT 121

Query: 152 MPKGRYKIMTNYMPKVGSYGLDMMYRTCTVQTNLDFSSE----------ADMVKKLRVSV 201
              GR+K +     K     L        V  N                A   + +R   
Sbjct: 122 SNTGRFKTLYREGLKNRYGALMQT--ISGVHYNFSLPMAFWQAKKEKISAGYFRVIRNYY 179

Query: 202 ALQPVATALFANSP-----FTEGKPNGFLSFRSEIWRDTDNARSGMIPWAFEDGM--GFE 254
               V   LF  SP     F    P               + R   + +  +  +   F 
Sbjct: 180 RFGWVIPYLFGASPAISSSFLTSLPFEKTESGMYYLPYATSLRLSDLGYTNQSNLGITFN 239

Query: 255 RWVDYALDVPMYFVKRGDHYIDVSGSSFRDFFDGKNEKLPGERPTLSDWANHLSTIFPEV 314
              +Y   +        + Y  +            +  L  +             +  E 
Sbjct: 240 DLYEYVAGLKQAIKTPSEEYAKIGIEKDGKRLQINSNVL--QIENELYAPIRPKRVTRES 297

Query: 315 RLKR-------YLEMRGADGVPWGRLPALPAFWVGLLYDDTSLDAAWEIVKGWDASERQA 367
                      Y+E+R  D  P+  +            D   +  A        +SE   
Sbjct: 298 PSDALLRGGIEYIEVRSLDINPFSPIGV--DEQQVRFLDLFMVWCALADAPEMSSSELAC 355

Query: 368 LRDDVPRLGFKARI-GNRFLFEIARECLVLAH------AGLRRRGRIDGFGHDETRHLAA 420
            R +  R+  + R  G             L          L+R  +     +    +   
Sbjct: 356 TRVNWNRVILEGRKPGLTLGIGCETAQFPLPQVGKDLFRDLKRVAQTLDSINGGEAYQKV 415

Query: 421 LDQILDA----GRTPAEEMLEKFDGAWRG 445
            D+++        T +  +L        G
Sbjct: 416 CDELVACFDNPDLTFSARILRSMIDTGIG 444


>2d32D [x.x.x] GLUTAMATE--CYSTEINE LIGASE
          Length = 504

 Score =  188 bits (477), Expect = 3e-50
 Identities = 61/450 (13%), Positives = 113/450 (25%), Gaps = 62/450 (13%)

Query: 44  LEGHHPVPYAGARGIGALLEGMQL-LLGWEPIMEGPHIIGLHDVTGGGAISLEPGGQFEL 102
           LE H        RG+    E +++   G       P  +G        +         + 
Sbjct: 12  LEKHPQALKGIQRGLE--RETLRVNADGTLATTGHPEALG--------SALTHKWITTDF 61

Query: 103 SGAPLD---NVHQTHAELMAHLAQVR-EVATPLGIGFLGLGMTP--SWSREDIPV----- 151
           + A L+    V      ++  +  +    A  +G   +     P      +DI +     
Sbjct: 62  AEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPLSMPSYIAEGQDIELAQYGT 121

Query: 152 MPKGRYKIMTNYMPKVGSYGLDMMYRTCTVQTNLDFSSEADMV-----------KKLRVS 200
              GR+K +     K       +M     V  N                     + +R  
Sbjct: 122 SNTGRFKTLYREGLKNR--YGALMQTISGVHYNFSLPMAFWQAKAKEKISAGYFRVIRNY 179

Query: 201 VALQPVATALFANSPFTE-----GKPNGFLSFRSEIWRDTDNARSGMIPWAFEDGMG--F 253
                V   LF  SP          P               + R   + +  +  +G  F
Sbjct: 180 YRFGWVIPYLFGASPAISSSFLTSLPFEKTESGMYYLPYATSLRLSDLGYTNQSNLGITF 239

Query: 254 ERWVDYALDVPMYFVKRGDHYIDVSGSSFRDFFDGKNEKLPGE-------RPTLSDWANH 306
               +Y   +        + Y  +            +  L  E       RP     +  
Sbjct: 240 NDLYEYVAGLKQAIKTPSEEYAKIGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGE 299

Query: 307 LSTIFPEVRLKRYLEMRGADGVPWGRLPALPAFWVGLLYDDTSLDAAWEIVKGWDASERQ 366
             +         Y+E+R  D  P+  +  +    V  L     +  A        +SE  
Sbjct: 300 SPSDALLRGGIEYIEVRSLDINPFSPI-GVDEQQVRFLD-LFMVWCALADAPEMSSSELA 357

Query: 367 ALRDDVPR-------LGFKARIGNRFLFEIARECLVLAHAGLRRRGRIDGFGHDETRHLA 419
             R +  R        G    IG         +        L+R  +     +    +  
Sbjct: 358 CTRVNWNRVILEGRKPGLTLGIGCETAQFPLPQVGKDLFRDLKRVAQTLDSINGGEAYQK 417

Query: 420 ALDQILDA----GRTPAEEMLEKFDGAWRG 445
             D+++        T +  +L        G
Sbjct: 418 VCDELVACFDNPDLTFSARILRSMIDTGIG 447


>2d33C [x.x.x] GLUTAMATE--CYSTEINE LIGASE
          Length = 504

 Score =  178 bits (451), Expect = 4e-47
 Identities = 55/482 (11%), Positives = 105/482 (21%), Gaps = 79/482 (16%)

Query: 8   MTPLNSRDELVGWIEAGVKPPSEFRIGTEHEKTPFTLEGHHPVPYAGARGIGALLEGMQL 67
           M P  S+   + W+E   +     + G E E      +G                     
Sbjct: 1   MIPDVSQ--ALAWLEKHPQALKGIQRGLERETLRVNADG--------------------- 37

Query: 68  LLGWEPIMEGPHIIGLHDVTGGGAISLE-PGGQFELSGAPLDNVHQTHAELMAHLAQVRE 126
                     P  +G         I+ +      E       ++      +         
Sbjct: 38  ---TLATTGHPEALGSAL--THKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTAR 92

Query: 127 VATPLGIGFLGLGMTPSWSREDIPVMPKGRYKIMTNYMPKVGSYGLDMMYR---TCTVQT 183
                 +  L +  +     +DI +   G                            V  
Sbjct: 93  NMGDERMWPLSMP-SYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHY 151

Query: 184 NLDFSSEA----------------DMVKKLRVSVALQPVATALFANSPFTEG-------- 219
           N                          + +R       V   LF  SP            
Sbjct: 152 NFSLPMAFWQAKISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAISSSFLSLPFE 211

Query: 220 KPNGFLSFRSEIWRDTDNARSGMIPWAFEDGMGFERWVDYALDVPMYFVKRGDHYIDVSG 279
           K   +L + + +        +  +   F D   +   +  A+  P     +     D   
Sbjct: 212 KTMYYLPYATSLRLSDLGYTNSNLGITFNDLYEYVAGLKQAIKTPSEEYAKIGIEKDGKR 271

Query: 280 SSFRD-FFDGKNEKLPGERPTLSDWANHLSTIFPEVRLKRYLEMRGADGVPWGRLPALPA 338
                     +NE     RP     +    +         Y+E+R  D  P+  +  +  
Sbjct: 272 LQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDINPFSPIG-VDE 330

Query: 339 FWVGLLYDDTSLDAAWEIVKGWDASERQALRDDVPR-------LGFKARIGNRF----LF 387
             V  L     +  A        +SE    R +  R        G    IG       L 
Sbjct: 331 QQVRFLD-LFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLGIGCETAQFPLP 389

Query: 388 EIARECLVLAHAGLRRRGRIDGFGHDETRHLAAL----DQILDAGRTPAEEMLEKFDGAW 443
           ++ ++         +         +    +            +   T +  +L       
Sbjct: 390 QVGKDLFRDLKRVAQTL----DSINGGEAYQKVCDELVACFDNPDLTFSARILRSMIDTG 445

Query: 444 RG 445
            G
Sbjct: 446 IG 447


>2d33B [x.x.x] GLUTAMATE--CYSTEINE LIGASE
          Length = 503

 Score =  170 bits (431), Expect = 8e-45
 Identities = 63/470 (13%), Positives = 111/470 (23%), Gaps = 76/470 (16%)

Query: 33  IGTEHEKTPFTLEGHHPVPYAGARGIGALLEGMQLLL-GWEPIMEGPHIIGLHDVTGGGA 91
           I    +   + LE H        RG+    E +++   G       P  +G        +
Sbjct: 2   IPDVSQALAW-LEKHPQALKGIQRGLE--RETLRVNADGTLATTGHPEALG--------S 50

Query: 92  ISLEPGGQFELSGAPLDNVHQTHAELMAHLAQVRE----VATPLGIGFLGLGMTPS--WS 145
                    + + A L+ +     ++   L  +R+     A  +G   +     PS    
Sbjct: 51  ALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPLSMPSYIAE 110

Query: 146 REDIPV---------MPKGRYKIMTNYMPKVGSYGLDMMYRTCTVQTNLDFSSEADMVKK 196
            +DI +           K  Y+           YG  M      V  N          K 
Sbjct: 111 GQDIELAQYGTSNTGRFKTLYREGLKNR-----YGALMQT-ISGVHYNFSLPMAFWQAKS 164

Query: 197 LRVSV------------ALQPVATALFANSPFTEGKPNGFLSFRSEIWRDTDNARSGMIP 244
            +  +                V   LF  SP      + FLS   E              
Sbjct: 165 AKEKISAGYFRVIRNYYRFGWVIPYLFGASPAIS---SSFLSLPFEKTESGMYYLPYATS 221

Query: 245 WAFED--------GMGFERWVDYALDVPMYFVKRGDHYIDVSGSSFRDFFDGKNEKLPGE 296
               D        G+ F    +Y   +        + Y  +            +  L  E
Sbjct: 222 LRLSDLGYTNSNLGITFNDLYEYVAGLKQAIKTPSEEYAKIGIEKDGKRLQINSNVLQIE 281

Query: 297 -------RPTLSDWANHLSTIFPEVRLKRYLEMRGADGVPWGRLPALPAFWVGLLYDDTS 349
                  RP     +    +         Y+E+R  D  P+  +            D   
Sbjct: 282 NELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDINPFSPIGVDEQQ--VRFLDLFM 339

Query: 350 LDAAWEIVKGWDASERQALRDDVPRLGFKARIGNRFLFEIARECLVLAHAGLRR------ 403
           +  A        +SE    R +  R+  + R     L               +       
Sbjct: 340 VWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLGIGCETAQFPLPQVGKDLFRDLK 399

Query: 404 -RGRIDGFGHDETRHLAALD----QILDAGRTPAEEMLEKFDGAWRGSVE 448
              +     +    +    D       +   T +  +L        G   
Sbjct: 400 RVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSARILRSMIDTGIGGTG 449


>2d32A [x.x.x] GLUTAMATE--CYSTEINE LIGASE
          Length = 510

 Score =  146 bits (369), Expect = 1e-37
 Identities = 57/440 (12%), Positives = 104/440 (23%), Gaps = 63/440 (14%)

Query: 64  GMQLLLGWEP----IMEGPHIIGLHDVTGGGAISLEPGGQFELSGAPLDNVHQTHAELMA 119
              +  G E     +     +         G+         + + A L+ +     ++  
Sbjct: 19  LKGIQRGLERETLRVNADGTLATTGHPEALGSALTHKWITTDFAEALLEFITPVDGDIEH 78

Query: 120 HLAQV----REVATPLGIGFLGLGMTPSWSREDIPV-------MPKGRYKIMTNYMPKVG 168
            L  +    R  A  +G   +     PS+  E   +          GR+K +     K  
Sbjct: 79  MLTFMRDLHRYTARNMGDERMWPLSMPSYIAEGQDIELAQYGTSNTGRFKTLYREGLKNR 138

Query: 169 SYGLDMMYRTCTVQTNLDFSSEADMV---------------KKLRVSVALQPVATALFAN 213
              L        V  N                         + +R       V   LF  
Sbjct: 139 YGALMQTI--SGVHYNFSLPMAFWQAKSGADAKEKISAGYFRVIRNYYRFGWVIPYLFGA 196

Query: 214 SPFTEGK----------PNGFL------SFRSEIWRDTDNARSGMIPWAFEDGMGFERWV 257
           SP                +G        S R      T+ ++S +    F D   +   +
Sbjct: 197 SPAISSSFLTSLPFEKTESGMYYLPYATSLRLSDLGYTNKSQSNL-GITFNDLYEYVAGL 255

Query: 258 DYALDVPMYFVKRGDHYIDVSGSSFRDF-FDGKNEKLPGERPTLSDWANHLSTIFPEVRL 316
             A+  P     +     D             +NE     RP     +    +       
Sbjct: 256 KQAIKTPSEEYAKIGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGG 315

Query: 317 KRYLEMRGADGVPWGRLPALPAFWVGLLYDDTSLDAAWEIVKGWDASERQALRDDVPRLG 376
             Y+E+R  D  P+  +            D   +  A        +SE    R +  R+ 
Sbjct: 316 IEYIEVRSLDINPFSPIGVDEQQ--VRFLDLFMVWCALADAPEMSSSELACTRVNWNRVI 373

Query: 377 FKARIGNRFLFEIARECLVLAH-------AGLRRRGRIDGFGHDETRHLAAL----DQIL 425
            + R     L                     L+R  +     +    +            
Sbjct: 374 LEGRKPGLTLGIGCETAQFPLPQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFD 433

Query: 426 DAGRTPAEEMLEKFDGAWRG 445
           +   T +  +L        G
Sbjct: 434 NPDLTFSARILRSMIDTGIG 453


  Database: 95pdb09Oct16_0000
    Posted date:  Oct 27, 2009  4:27 PM
  Number of letters in database: 15,983,541
  Number of sequences in database:  66,450
  
Lambda     K      H
   0.323    0.136    0.402 

Gapped
Lambda     K      H
   0.267   0.0499    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 66450
Number of Hits to DB: 27,589,258
Number of extensions: 1775321
Number of successful extensions: 19225
Number of sequences better than 1.0e-03: 10
Number of HSP's gapped: 8096
Number of HSP's successfully gapped: 626
Length of database: 100,000
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.5 bits)