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Agrobacterium tumefaciens str. C58 (atum0)
Chromosome : circular
Gene : miaB
DDBJ      :miaB         miaB protein
Swiss-Prot:MIAB_AGRT5   RecName: Full=(Dimethylallyl)adenosine tRNA methylthiotransferase miaB;         EC=2.-.-.-;AltName: Full=tRNA-i(6)A37 methylthiotransferase;

Homologs  Archaea  67/68 : Bacteria  791/915 : Eukaryota  105/199 : Viruses  0/175   --->[See Alignment]
b.40.4c.1.28c.92.1
:455 amino acids
:SECSTR
:PSIPRED
:DISOPRED
:BLT:PDB   177->440 2qgqC PDBj 4e-17 35.8 %
:RPS:PDB   10->309 2d32D PDBj 3e-50 10.7 %
:RPS:PDB   280->371 3dcxA PDBj 4e-21 9.9 %
:RPS:SCOP  146->371 1qgoA  c.92.1.2 * 4e-32 9.2 %
:RPS:SCOP  390->452 1yezA1  b.40.4.12 * 3e-07 23.7 %
:HMM:SCOP  107->385 1oltA_ c.1.28.2 * 2e-59 30.2 %
:RPS:PFM   12->112 PF00919 * UPF0004 9e-19 46.8 %
:RPS:PFM   165->333 PF04055 * Radical_SAM 2e-14 43.0 %
:HMM:PFM   166->338 PF04055 * Radical_SAM 3.7e-35 34.8 158/166  
:HMM:PFM   11->114 PF00919 * UPF0004 1.8e-32 48.0 98/98  
:HMM:PFM   393->452 PF01938 * TRAM 1.5e-12 35.6 59/61  
:BLT:SWISS 1->455 MIAB_AGRT5 0.0 100.0 %
:PROS 165->185|PS01278|MTTASE_RADICAL
:SEG

SeqInfo AminoSeq See neighboring genes
Links GIB DAD Abbreviations Back to title page
GT:ID AAK86173.2 GT:GENE miaB GT:PRODUCT miaB protein GT:DATABASE GIB00072CH01 GT:ORG atum0 GB:ACCESSION GIB00072CH01 GB:CHROMOSOME circular GB:LOCATION 349679..351046 GB:FROM 349679 GB:TO 351046 GB:DIRECTION + GB:GENE miaB GB:PRODUCT miaB protein GB:PROTEIN_ID AAK86173.2 GB:DB_XREF GI:159139599 GB:GENE:GENE miaB LENGTH 455 SQ:AASEQ MIAREGSNSRKVFIKTYGCQMNVYDSVRMSDALAKDGYVQTEDMGEADLVLLNTCHIREKAAEKVYSALGRLRDMKKSREEQGREFMIGVAGCVAQAEGEEILRRAPAVDVVIGPQTYHRLPDALKRVRRGERVIETEYAVEDKFEHLPVAEKATLRSRGVTAFLTVQEGCDKFCTFCVVPYTRGSEVSRPVRQIVDEAMKLVDAGVREITLLGQNVNAWQGEGPKGEKWGLAELLYRLAEIPGLARLRYTTSHPRDMDDRLIGAHRDLRILMPYLHLPVQSGSDRILKAMNRRHTGEEYIQLIEKIRSARPDIAMSGDFIVGFPGETDRDFEDTMAMVETVKYAQAFSFKYSTRPGTPGADLTDQVAEDVKAERLERLQALLLRQQKEFAESLVGKTMDVLLEKPGRMPEQLIGRSPWLQSVNLDAKTLKIGDIVNVRITATGPNSLFAEVAGS GT:EXON 1|1-455:0| SW:ID MIAB_AGRT5 SW:DE RecName: Full=(Dimethylallyl)adenosine tRNA methylthiotransferase miaB; EC=2.-.-.-;AltName: Full=tRNA-i(6)A37 methylthiotransferase; SW:GN Name=miaB; OrderedLocusNames=Atu0356; ORFNames=AGR_C_623; SW:KW 4Fe-4S; Complete proteome; Cytoplasm; Iron; Iron-sulfur;Metal-binding; S-adenosyl-L-methionine; Transferase; tRNA processing. SW:EXACT T SW:FUNC + BL:SWS:NREP 1 BL:SWS:REP 1->455|MIAB_AGRT5|0.0|100.0|455/455| GO:SWS:NREP 6 GO:SWS GO:0051539|"GO:4 iron, 4 sulfur cluster binding"|4Fe-4S| GO:SWS GO:0005737|"GO:cytoplasm"|Cytoplasm| GO:SWS GO:0051536|"GO:iron-sulfur cluster binding"|Iron-sulfur| GO:SWS GO:0046872|"GO:metal ion binding"|Metal-binding| GO:SWS GO:0016740|"GO:transferase activity"|Transferase| GO:SWS GO:0008033|"GO:tRNA processing"|tRNA processing| PROS 165->185|PS01278|MTTASE_RADICAL|PDOC00984| SEG 372->389|kaerlerlqalllrqqke| BL:PDB:NREP 1 BL:PDB:REP 177->440|2qgqC|4e-17|35.8|243/263| RP:PDB:NREP 2 RP:PDB:REP 10->309|2d32D|3e-50|10.7|290/504| RP:PDB:REP 280->371|3dcxA|4e-21|9.9|91/114| RP:PFM:NREP 2 RP:PFM:REP 12->112|PF00919|9e-19|46.8|94/97|UPF0004| RP:PFM:REP 165->333|PF04055|2e-14|43.0|149/164|Radical_SAM| HM:PFM:NREP 3 HM:PFM:REP 166->338|PF04055|3.7e-35|34.8|158/166|Radical_SAM| HM:PFM:REP 11->114|PF00919|1.8e-32|48.0|98/98|UPF0004| HM:PFM:REP 393->452|PF01938|1.5e-12|35.6|59/61|TRAM| GO:PFM:NREP 5 GO:PFM GO:0003824|"GO:catalytic activity"|PF00919|IPR013848| GO:PFM GO:0009451|"GO:RNA modification"|PF00919|IPR013848| GO:PFM GO:0051539|"GO:4 iron, 4 sulfur cluster binding"|PF00919|IPR013848| GO:PFM GO:0003824|"GO:catalytic activity"|PF04055|IPR007197| GO:PFM GO:0051536|"GO:iron-sulfur cluster binding"|PF04055|IPR007197| RP:SCP:NREP 2 RP:SCP:REP 146->371|1qgoA|4e-32|9.2|206/257|c.92.1.2| RP:SCP:REP 390->452|1yezA1|3e-07|23.7|59/68|b.40.4.12| HM:SCP:REP 107->385|1oltA_|2e-59|30.2|262/0|c.1.28.2|1/1|Radical SAM enzymes| OP:NHOMO 2068 OP:NHOMOORG 963 OP:PATTERN 1112111111111111111111111111-111112322323121112322212222221111111111 2331311111111111111-111121111111111111112222211211111111111122222222222111111133331333333333233322233333333313-------22222223333333333332222222222222222222222222222222222222212222212222233443222222222222222222222222222222221-------3222222222222222222221----------------------------------------------------------------------33333333333333343333333333222332333334334333333321233212223323333332233333333333323333-3333333323212222222222223333233333333332222222222222333111222222222222222222222222221333332222222222222222222222222222222222222222222222222222222222111111122222233333333333333333333333322322233333333333333333333333333322222222212112222211122221211211111121211111122222112222222222-2222222222222222222222221122222222222222222122122221-111111111111---31111122223121122222222112222222232222222222221222211112222211122121211112222211122222222222211111133333333--------32-------------------2------4334443444222 1111112-1-11111----------------------------------------------------------------------------------------111-131472222-222221242252AR3-6262211622222212121252223111523212222142523334T444442223-253352223 ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- STR:NPRED 449 STR:RPRED 98.7 SQ:SECSTR EEEETTccHcTGGGGccEEEEEEEccHHHHTTccHHHHHHHHHHHHTTHHHHHHccccEEcGGGccccccEEcTTccEEcTTcccGGGcTTcccccGGGccccccHHHHHHHHcccHHHHTTccEETTEEcccccGGGccccEEEEccccTTccHHHHHHHHcccEEEEEEEccTTccccHHHHHcccccccHHHHHHTHHHHHHHHHHTTcTTcEEccTTccccEEHHHHHHHHHHHHHHHHHHHHHHHcccHHHHHEHHHHHHHGGHHHHHHHHHcGGGcHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEcEEETTEEEEEcTTcccccHHHHHHHHHHTTccccEEEcccGGGcccHHHHHHHHHHHHHHTTcTTccGGGccEEEEEETTTTEEEEccccEEEEEcTTEEEEEcTTHHHHHHHHHHHHHHHccHHHHHHHHHHHTT###### DISOP:02AL 1-12,455-456| PSIPRED ccccccccccEEEEEEccccccHHHHHHHHHHHHHccccccccHHHccEEEEEccccccHHHHHHHHHHHHHHHHHHHHHHHcccEEEEEccccccccHHHHHHHcccccEEEccccHHHHHHHHHHHHcccccEEccccccccccccccccccccccccEEEEEEEcccccccccEEEEEEEEccEEcccHHHHHHHHHHHHHccccEEEEEEEcccccccccccccHHHHHHHHHHHHHcccccEEEEEcccHHHccHHHHHHHHHccccccEEEEccccccHHHHHHHcccccHHHHHHHHHHHHHHccccEEEEEEEEEcccccHHHHHHHHHHHHHccccEEEEEEEEccccHHHHHccccccHHHHHHHHHHHHHHHHHHHHHHHHHHcccEEEEEEEEEcccccEEEEEcccccEEEEcccccccccEEEEEEEEEEccEEEEEEccc //