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Clostridium perfringens str. 13 (cper0)
Gene : CPE1093
DDBJ      :CPE1093      conserved hypothetical protein
Swiss-Prot:MIAB_CLOPE   RecName: Full=(Dimethylallyl)adenosine tRNA methylthiotransferase miaB;         EC=2.-.-.-;AltName: Full=tRNA-i(6)A37 methylthiotransferase;

Homologs  Archaea  67/68 : Bacteria  791/915 : Eukaryota  106/199 : Viruses  0/175   --->[See Alignment]
b.40.4c.1.28c.92.1
:447 amino acids
:SECSTR
:PSIPRED
:DISOPRED
:BLT:PDB   171->413 2qgqC PDBj 4e-27 33.1 %
:RPS:PDB   202->435 2btvB PDBj 6e-47 11.3 %
:RPS:SCOP  155->396 1qgoA  c.92.1.2 * 1e-38 12.7 %
:RPS:SCOP  375->447 1yezA1  b.40.4.12 * 2e-10 23.5 %
:HMM:SCOP  105->377 1oltA_ c.1.28.2 * 6.4e-60 29.4 %
:RPS:PFM   16->111 PF00919 * UPF0004 8e-14 41.8 %
:RPS:PFM   159->323 PF04055 * Radical_SAM 1e-11 34.0 %
:RPS:PFM   388->445 PF01938 * TRAM 5e-06 46.4 %
:HMM:PFM   159->330 PF04055 * Radical_SAM 1.1e-37 34.2 158/166  
:HMM:PFM   13->112 PF00919 * UPF0004 4.4e-31 45.8 96/98  
:HMM:PFM   385->446 PF01938 * TRAM 4.9e-17 45.0 60/61  
:BLT:SWISS 1->447 MIAB_CLOPE 0.0 100.0 %
:PROS 159->179|PS01278|MTTASE_RADICAL
:SEG

SeqInfo AminoSeq See neighboring genes
Links GIB DAD Abbreviations Back to title page
GT:ID BAB80799.1 GT:GENE CPE1093 GT:PRODUCT conserved hypothetical protein GT:DATABASE GIB00075CH01 GT:ORG cper0 GB:ACCESSION GIB00075CH01 GB:LOCATION 1294599..1295942 GB:FROM 1294599 GB:TO 1295942 GB:DIRECTION + GB:GENE CPE1093 GB:PRODUCT conserved hypothetical protein GB:NOTE 447 aa, similar to sp:YMCB_BACSU HYPOTHETICAL 58.2 KDA PROTEIN IN KLB-COTE INTERGENIC REGION from Bacillus subtilis (509 aa); 50.3% identity in 439 aa overlap GB:PROTEIN_ID BAB80799.1 LENGTH 447 SQ:AASEQ MTLENNMDKKLFCISTYGCQMNEEDSEKLSGMLKSQGYERTENKEEASIIIFNTCCVRENAENKVFGNLGQLKQLKKKNPNLVIAICGCMMQQVGMADKVLKTFPYVDIIFGTHNAHKFPEYLHRVLQEGVQVKEILNKEEGIVEGLPIDRKSDVKAFVTIMYGCNNFCTYCIVPYVRGRERSRKSEDIIKEIEELVSQGYKEITLLGQNVNSYGKGLEEDIDFAGLLRKVNEVKGLERVRFMTSHPKDLSDDVIMAIKECDKLCEQVHLPVQSGSSRILKEMNRHYDREYYLDLVKKIKSEIPDVTLTTDIIIGFPGETEEDFLDTLSLCEEVGYDSAFTFIYSRRNHTPADKMENQIPDDIKHDRFNRLVEAINKKVVIKNKEYEGKVVEVLVEGPSKNDETKLTGRTRNGKLVNFAGDEKLVGELVNLKIVRAQPFSLIGEIVE GT:EXON 1|1-447:0| SW:ID MIAB_CLOPE SW:DE RecName: Full=(Dimethylallyl)adenosine tRNA methylthiotransferase miaB; EC=2.-.-.-;AltName: Full=tRNA-i(6)A37 methylthiotransferase; SW:GN Name=miaB; OrderedLocusNames=CPE1093; SW:KW 4Fe-4S; Complete proteome; Cytoplasm; Iron; Iron-sulfur;Metal-binding; S-adenosyl-L-methionine; Transferase; tRNA processing. SW:EXACT T SW:FUNC + BL:SWS:NREP 1 BL:SWS:REP 1->447|MIAB_CLOPE|0.0|100.0|447/447| GO:SWS:NREP 6 GO:SWS GO:0051539|"GO:4 iron, 4 sulfur cluster binding"|4Fe-4S| GO:SWS GO:0005737|"GO:cytoplasm"|Cytoplasm| GO:SWS GO:0051536|"GO:iron-sulfur cluster binding"|Iron-sulfur| GO:SWS GO:0046872|"GO:metal ion binding"|Metal-binding| GO:SWS GO:0016740|"GO:transferase activity"|Transferase| GO:SWS GO:0008033|"GO:tRNA processing"|tRNA processing| PROS 159->179|PS01278|MTTASE_RADICAL|PDOC00984| SEG 67->82|gnlgqlkqlkkknpnl| BL:PDB:NREP 1 BL:PDB:REP 171->413|2qgqC|4e-27|33.1|239/263| RP:PDB:NREP 1 RP:PDB:REP 202->435|2btvB|6e-47|11.3|212/885| RP:PFM:NREP 3 RP:PFM:REP 16->111|PF00919|8e-14|41.8|91/97|UPF0004| RP:PFM:REP 159->323|PF04055|1e-11|34.0|150/164|Radical_SAM| RP:PFM:REP 388->445|PF01938|5e-06|46.4|56/59|TRAM| HM:PFM:NREP 3 HM:PFM:REP 159->330|PF04055|1.1e-37|34.2|158/166|Radical_SAM| HM:PFM:REP 13->112|PF00919|4.4e-31|45.8|96/98|UPF0004| HM:PFM:REP 385->446|PF01938|4.9e-17|45.0|60/61|TRAM| GO:PFM:NREP 5 GO:PFM GO:0003824|"GO:catalytic activity"|PF00919|IPR013848| GO:PFM GO:0009451|"GO:RNA modification"|PF00919|IPR013848| GO:PFM GO:0051539|"GO:4 iron, 4 sulfur cluster binding"|PF00919|IPR013848| GO:PFM GO:0003824|"GO:catalytic activity"|PF04055|IPR007197| GO:PFM GO:0051536|"GO:iron-sulfur cluster binding"|PF04055|IPR007197| RP:SCP:NREP 2 RP:SCP:REP 155->396|1qgoA|1e-38|12.7|221/257|c.92.1.2| RP:SCP:REP 375->447|1yezA1|2e-10|23.5|68/68|b.40.4.12| HM:SCP:REP 105->377|1oltA_|6.4e-60|29.4|262/0|c.1.28.2|1/1|Radical SAM enzymes| OP:NHOMO 2065 OP:NHOMOORG 964 OP:PATTERN 1121111111111111111111111111-111112442323221122322212311111111111111 2331311111111111111-111121111111111111112222211211111111111122222222222111111133331333333333233322233333333313-------22222223333333333332222222222222222222222222222222222222212222212222233333222222222222222222222222222222221-------3222222222222222222221----------------------------------------------------------------------33333333333333343333333333222332333334334333333321233212223323333332233333333333323333-3333333323212222222222223333233333333332222222222222333111222222222222222222222222221333332222222222222222222222222222222222222222222222222222222222111111122222233333333333333333333333322222233333333333333333333333333322222222212112222211122221212211111121211111122222112222222222-2222222222222222222222221122222222222222222122122221-111111111111---31111122223121122222222112222222232222222222221222211112222211122121211112222211122222222222211111133333333--------32-------------------2------3433343344222 1111112-1-11111----------------------------------------------------------------------------------------111-1314822222222221232252AR3-6261221521222222221252323111523212222142523334T444442223-223352223 ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- STR:NPRED 446 STR:RPRED 99.8 SQ:SECSTR #ETTccHHHHcccGGccEEEEEEEccHHHHTTccHHHHHHHHHHHHTTHHHHHHccccTTccHHHHTcHHHHHHTccccHHHcEEEEHHHHTccEETTccccEEEEGGGcccccccccHHHHHHHHHHTTccEEEEEccccHHHHGGGGccHHHHHHHHHHHHHHccEEEEEEEGGGGTccTTcccHHHHHHHHHHHHHHHcTTTccccGccEEEEHHHHHHHHHHHHHHHHHHHTccccccccccHHHHHHHHHTTTTcHHccccccccccccEEEEEccccccEEEEEHHHHHHHTcccEEEcEEETTEEEEccccccHHHHHHHHHHHHHHHHHHccccccccEEEETTEEEEcHHGGGccEEcTTTGGGccHHHHHHHHHHHHHHHTTcccTTcTTccccEEEccccccHHHEHHHHHHHHHHHHHccHHHHHHHHTTcEEcc DISOP:02AL 1-9| PSIPRED ccccccccccEEEEEEccccccHHHHHHHHHHHHHcccccccccccccEEEEEcccEEcHHHHHHHHHHHHHHHHHHHcccEEEEEEcccccccccHHHHHHHccccEEEEccccHHHHHHHHHHHHHccccccccccccccccccccccccccEEEEEEEcccccccccEEEEcccccccccccHHHHHHHHHHHHHccccEEEEEEcccccccccccccccHHHHHHHHHHHccccEEEEEccccccccHHHHHHHHHcccEEcEEEEEEEcccHHHHHHHcccccHHHHHHHHHHHHHHccccEEEEEEEEEcccccHHHHHHHHHHHHHHcccEEEEEEEEccccccHHHccccccHHHHHHHHHHHHHHHHHHHHHHHHHHcccEEEEEEEEcccccccEEEEEccccEEEEEcccccccccEEEEEEEEEcccEEEEEEEc //