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Francisella tularensis subsp. mediasiatica FSC147 (ftul2)
Gene : miaB
DDBJ      :miaB         tRNA-methylthiotransferase MiaB protein
Swiss-Prot:MIAB_FRATW   RecName: Full=(Dimethylallyl)adenosine tRNA methylthiotransferase miaB;         EC=2.-.-.-;AltName: Full=tRNA-i(6)A37 methylthiotransferase;

Homologs  Archaea  67/68 : Bacteria  791/915 : Eukaryota  104/199 : Viruses  0/175   --->[See Alignment]
b.40.4c.1.15c.1.28
:442 amino acids
:SECSTR
:PSIPRED
:DISOPRED
:BLT:PDB   151->431 2qgqA PDBj 2e-20 40.8 %
:RPS:PDB   94->359 3cqiA PDBj 5e-54 13.7 %
:RPS:SCOP  96->353 1qtwA  c.1.15.1 * 2e-41 12.3 %
:RPS:SCOP  375->442 1yezA1  b.40.4.12 * 3e-10 14.3 %
:HMM:SCOP  99->373 1oltA_ c.1.28.2 * 1.7e-61 31.3 %
:RPS:PFM   7->105 PF00919 * UPF0004 1e-16 46.8 %
:RPS:PFM   155->324 PF04055 * Radical_SAM 4e-09 35.3 %
:RPS:PFM   381->441 PF01938 * TRAM 2e-08 50.8 %
:HMM:PFM   153->327 PF04055 * Radical_SAM 1.9e-31 33.1 160/166  
:HMM:PFM   6->106 PF00919 * UPF0004 1.9e-30 45.9 98/98  
:HMM:PFM   380->442 PF01938 * TRAM 8.1e-19 44.3 61/61  
:BLT:SWISS 1->442 MIAB_FRATW 0.0 100.0 %
:PROS 153->173|PS01278|MTTASE_RADICAL
:SEG

SeqInfo AminoSeq See neighboring genes
Links GIB DAD Abbreviations Back to title page
GT:ID ACD30672.1 GT:GENE miaB GT:PRODUCT tRNA-methylthiotransferase MiaB protein GT:DATABASE GIB00719CH01 GT:ORG ftul2 GB:ACCESSION GIB00719CH01 GB:LOCATION complement(757642..758970) GB:FROM 757642 GB:TO 758970 GB:DIRECTION - GB:GENE miaB GB:PRODUCT tRNA-methylthiotransferase MiaB protein GB:PROTEIN_ID ACD30672.1 GB:DB_XREF GI:187712375 GB:GENE:GENE miaB LENGTH 442 SQ:AASEQ MKEQKKVFIKTLGCQMNEYDSARMHEVLNEHFDTVKTDDYKDADIILINTCSIREKAQEKVFHELGRWKGLKKTNEDLIIGVGGCVASQEGENIIKRAPFVDLVFGPQTIHRLPEMIKQKQKTQQSQVDISFPEVEKFDYLPEPKAEGAKAYVSIMEGCDKYCSYCVVPYTRGPEVNRPFEDVLAECAILAEQGVKEITLLGQNVNHYLGPMENGQTADLALLIHFIAEIDGIERIRFTTSHPVEFSQNLIDAYATVPELANHLHLPVQHGSDRILINMKRNHTILEFKQKIRKLRAIRPDITISSDFIVGFPGETEEDFQKLLDLVKEINFDQSFSFIYSKRPGTPAADLPDDTPMEVKKDRLKRLQDLLNSNAQIISRQMVGTNQRILVDGTSKKDDNILSGRTENNRVVNFKGDKSLIGQFAMVKITESLPNSLRGELI GT:EXON 1|1-442:0| SW:ID MIAB_FRATW SW:DE RecName: Full=(Dimethylallyl)adenosine tRNA methylthiotransferase miaB; EC=2.-.-.-;AltName: Full=tRNA-i(6)A37 methylthiotransferase; SW:GN Name=miaB; OrderedLocusNames=FTW_1110; SW:KW 4Fe-4S; Complete proteome; Cytoplasm; Iron; Iron-sulfur;Metal-binding; S-adenosyl-L-methionine; Transferase; tRNA processing. SW:EXACT T SW:FUNC + BL:SWS:NREP 1 BL:SWS:REP 1->442|MIAB_FRATW|0.0|100.0|442/442| GO:SWS:NREP 6 GO:SWS GO:0051539|"GO:4 iron, 4 sulfur cluster binding"|4Fe-4S| GO:SWS GO:0005737|"GO:cytoplasm"|Cytoplasm| GO:SWS GO:0051536|"GO:iron-sulfur cluster binding"|Iron-sulfur| GO:SWS GO:0046872|"GO:metal ion binding"|Metal-binding| GO:SWS GO:0016740|"GO:transferase activity"|Transferase| GO:SWS GO:0008033|"GO:tRNA processing"|tRNA processing| PROS 153->173|PS01278|MTTASE_RADICAL|PDOC00984| SEG 360->371|kkdrlkrlqdll| BL:PDB:NREP 1 BL:PDB:REP 151->431|2qgqA|2e-20|40.8|245/266| RP:PDB:NREP 1 RP:PDB:REP 94->359|3cqiA|5e-54|13.7|263/277| RP:PFM:NREP 3 RP:PFM:REP 7->105|PF00919|1e-16|46.8|94/97|UPF0004| RP:PFM:REP 155->324|PF04055|4e-09|35.3|156/164|Radical_SAM| RP:PFM:REP 381->441|PF01938|2e-08|50.8|59/59|TRAM| HM:PFM:NREP 3 HM:PFM:REP 153->327|PF04055|1.9e-31|33.1|160/166|Radical_SAM| HM:PFM:REP 6->106|PF00919|1.9e-30|45.9|98/98|UPF0004| HM:PFM:REP 380->442|PF01938|8.1e-19|44.3|61/61|TRAM| GO:PFM:NREP 5 GO:PFM GO:0003824|"GO:catalytic activity"|PF00919|IPR013848| GO:PFM GO:0009451|"GO:RNA modification"|PF00919|IPR013848| GO:PFM GO:0051539|"GO:4 iron, 4 sulfur cluster binding"|PF00919|IPR013848| GO:PFM GO:0003824|"GO:catalytic activity"|PF04055|IPR007197| GO:PFM GO:0051536|"GO:iron-sulfur cluster binding"|PF04055|IPR007197| RP:SCP:NREP 2 RP:SCP:REP 96->353|1qtwA|2e-41|12.3|235/285|c.1.15.1| RP:SCP:REP 375->442|1yezA1|3e-10|14.3|63/68|b.40.4.12| HM:SCP:REP 99->373|1oltA_|1.7e-61|31.3|262/0|c.1.28.2|1/1|Radical SAM enzymes| OP:NHOMO 2060 OP:NHOMOORG 962 OP:PATTERN 1112111111111111111111111111-111112222222221122321112311111111111111 2331311111111111111-111121111111111111112222211211111111111122222222222111111133331333333333233322233333333313-------22222223333333333332222222222222222222222222222222222222212222212222233333222222222222222222222222222222221-------3222222222222222222221----------------------------------------------------------------------33333333333333343333333333222332333334334333333321233212223323333332233333333333323333-3333333323212222222222223333233333333332222222222222333111222222222222222222222222221333332222222222222222222222222222222222222222222222222222222222111111122222233333333333333333333333322222233333333333333333333333333322222222212112222211122221211211111121211111122222112222222222-2222222222222222222222221122222222222222222122122221-111111111111---31111122223121122222222112222222232222222222221222211112222211122121211112222211122222222222211111133333333--------32-------------------2------3334443444222 1111112-1-11111----------------------------------------------------------------------------------------111-131482222-222221232252AR3-6261221521222222221252222111523212222-42523334T444442223-293352223 ------------------------------------------------------------------------------------------------------------------------------------------------------------------------------- STR:NPRED 442 STR:RPRED 100.0 SQ:SECSTR HHHHccccccccEEccEEEEEccTTccGGGcccccHHHTTccHHHHHHHHHHHHTcEEEEEEEcccTTcccccccHHHHHHHHHTcTTccEEEcccEEEEGGGccccHHHccHHHHHHHHHHTTccEEEEEccccHHHHGGGGccHHHHHHHHHHHHHHccEEEEEEEGGGGTccTTcccHHHHHHHHHHHHHHHHHHHHHTccEEEEcccccccccHHHHHHHHHHHHHHHHHHHHTcEEEEEccccGGGccHHHHHHHHHHHccTEEEEccHHHHHcccccHHHHHHHHTTTcccEEEcEEETTEEEEEcTTcccccHHHHHHHHHHTTccccEEEcccGGGcccHHHHHHHHHHHHHHTTcTTccGGGccEEEEEETTTTEEEEEEccccEEEEETTEEEEEcTTccEEEEEccTTccccHcHHHHHHHGGGcEEcEEc DISOP:02AL 442-443| PSIPRED cccccEEEEEEccccccHHHHHHHHHHHHHcccccccccHHHccEEEEEccccccHHHHHHHHHHHHHHHHHHHccccEEEEEcccccccHHHHHHHccccEEEEccccHHHHHHHHHHHHcccccccccccccccccccccccccccEEEEEEEccccccccEEEEEEEEEcccccccHHHHHHHHHHHHHccccEEEEEEccccccccccccccHHHHHHHHHHHHccccccEEEEEccccccccHHHHHHHHHcccccccEEEccccccHHHHHHHcccccHHHHHHHHHHHHHHccccEEEEEEEEEcccccHHHHHHHHHHHHHccccEEEEEEEccccccHHHHccccccHHHHHHHHHHHHHHHHHHHHHHHHHHcccEEEEEEEccccccccEEEEEcccccEEEEcccccccccEEEEEEEEEEccEEEEEEc //